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2024 Vol. 54, No. 3 Published: 2024-03-25   173 Wogonin protects hypoxia-induced pulmonary hypertension via regulating miR-451/PI3K/AKT/RXRA/Bcl-2 signaling pathway WU Peiliang, WANG Liangxing, HUANG Xiaoying. DOI: 10.3969/j.issn.2095-9400.2024.03.001 Objective: To evaluate the potential protective effect of wogonin on hypoxic pulmonary hypertension (HPH) and to elucidate the molecular mechanism underlying these advantageous outcomes.Methods: In vivo,18 C57BL/6 male wild-type mice were randomly divided as normoxia group (N), hypoxiagroup (H+Saline) and hypoxia+wogonin group (H+w). The mouse hemodynamics were detected using a pressuresensor system 21 days after modeling. The degree of pulmonary artery remodeling was calculated by wall area/total area and wall thickness/total thickness. Prediction and screening of potential targets and molecular signalingpathways of wogonin in hypoxic pulmonary hypertension were performed based on network pharmacology. In vitro, mouse pulmonary artery smooth muscle cells (MPASMCs) were cultured under the respective condition of normoxia (N), hypoxia (H+PBS), hypoxia+low dose wogonin (H+40 μmol/L w), hypoxia+high dose wogonin (H+80 μmol/L w), hypoxia+high dose wogonin (H+80 μmol/L w)+740Y-P (20 μg/mL). The N group was exposed to normoxic (5% CO2, 21% O2, 74% N2, 37 ℃) environment for 48 h. The H+PBS, H+40 μmol/L w, H+80 μmol/L w and H+80 μmol/L w+740Y-P groups were exposed to hypoxic (5% CO2, 3% O2, 92% N2, 37 ℃) environment for 48 h. Cell proliferation was assessed with the CCK-8 and EdU assay, respectively. The migration capacity of MPASMCs was determined by transwell and scratch wound healing assays. Protein expression profiles of the PI3K/AKT/RXRA/Bcl-2 signaling pathway (P-PI3K, PI3K, P-AKT, AKT, RXRA, Bcl-2) were analyzed using the Western blot method and the PI3K pathway activator 740Y-P was used for functional complementation experiments. The expression level of miR-451 was detected using RT-qPCR to assess the impact of wogonin.The expression of CAB39 and MIF, upstream regulatory factors of the PI3K/AKT/RXRA/Bcl-2 pathway, was examined by Western blot to detect the effect of wogonin on their regulation. Results: Comparative to the controls,hypoxic MPASMCs showed significantly increased proliferation and migration abilities (P<0.05). Moreover,the hypoxia group exhibited a significant increase in right ventricular systolic pressure (RVSP) and pulmonary vascular remodeling in comparison to the control group (P<0.05). After intervention with wogonin, the proliferation and migration activity of MPASMCs, RVSP and pulmonary vascular remodeling were rescued (P<0.05). The hypoxia group exhibited a significant increase in the expressions of p-PI3K, p-AKT, RXRA, and Bcl-2, which are proteins related to the PI3K/AKT/RXRA/Bcl-2 signaling pathway, when compared to the normoxia group.However, after wogonin intervention, the PI3K/AKT/RXRA/Bcl-2 signaling pathway was significantly inhibited(P<0.05). Functional restoration experiments confirmed that under hypoxic conditions, activation of the PI3K pathway can significantly weaken the inhibitory effect of wogonin on the proliferation of MPASMCs. In the context of the PI3K/AKT/RXRA/Bcl-2 pathway, it has been observed that wogonin exhibited a notable inhibitory effect on the upstream regulators CAB39 and MIF expression. Compared with to the normoxia group, the expression levels of CAB39 and MIF exhibited a significant up-regulation, while the expression of miR-451 demonstrated a significant downregulation in the hypoxia group (P<0.05). After wogonin intervention, the expression levels of CAB39, MIF and miR-451 were reversed. Further experimentation suggested that the intervention of a miR- 451 inhibitor demonstrated a partial reversal of the inhibitory impact exerted by wogonin on the proliferation and migration of MPASMCs (P<0.05). Conclusion: Wogonin has the ability to hinder the PI3K/AKT/RXRA/Bcl-2 signaling pathway through the up-regulation of miR-451 expression. This intervention leads to the amelioration of abnormal proliferation and migration in hypoxic MPASMCs, as well as the remodeling of pulmonary vascular structure in mice afflicted with HPH. Consequently, the administration of wogonin results in a reduction of pulmonary hypertension. 2024 Vol. 54 (3): 173-183 [Abstract] ( 247 ) HTML (1 KB)  PDF (3033 KB)  ( 543 ) 184 Preparation and identification of a polyclonal antibody against Pgp3 of Chlamydia trachomatis ZHOU Luqi, YANG Jia, LI Yang, ZHU Shanli. DOI: 10.3969/j.issn.2095-9400.2024.03.002 Objective: To prepare a rabbit polyclonal antibody against Pgp3 protein of Chlamydia trachomatis (Ct) and determine its biological function in vitro. Methods: Recombinant protein Pgp3 was obtained by prokaryotic expression system and purified by nickel ion affinity chromatography. After renaturing in Phosphate buffered solution, the renatured Pgp3 protein was injected subcutaneously into Japanese-white-rabbits at multiple sites to generate polyclonal antibody. The titer and specificity of the polyclonal antibody was assessed by ELISA and Western blot, respectively. The specific recognition ability of the polyclonal antibody to natural Pgp3 protein was tested by indirect immunofluorescence assay (IFA) with Ct-infected HeLa cell model and Western blot under non-denaturing conditions. Results: The Pgp3 protein was successfully obtained and mainly expressed in the form of inclusion body. After immunization, the rabbits produced a high level of polyclonal antibody with a titer reached 1:512 000. Western blot and IFA showed that the rabbit polyclonal antibody against Pgp3 specifically recognized both the recombinant and native Pgp3 protein. Conclusion: The study successfully generated a high level of polyclonal antibody against Pgp3, which exhibited specific recognition abilities for both recombinant and native Pgp3 protein. These results provide a foundation of further research on the biological function of Pgp3 and the development of Ct immunotherapy agents. 2024 Vol. 54 (3): 184-189 [Abstract] ( 182 ) HTML (1 KB)  PDF (1759 KB)  ( 614 ) 190 NLRP3 inflammasome inhibitor MCC950 ameliorates sepsis-associated organ damage via suppressing Th1/Th17 response and promoting Tregs response in mice YE Yumei, CHEN Longwang, ZHANG Wanli,WANG Xuetao. DOI: 10.3969/j.issn.2095-9400.2024.03.003 Objective: To investigate the effects of MCC950, the NLRP3 inflammasome inhibitor, on T cell differentiation and organ damage in septic mice. Methods: Eighteen C57BL/6 male mice were randomly divided into sham group, CLP group and MCC950 group, with six in each. In CLP group and MCC950 group, the sepsis model was operated by cecal ligation and puncture (CLP). MCC950 group was injected intraperitoneally with MCC950 (50 mg/kg) dissolved in 0.5 mL saline, and the sham group and CLP group were injected withan equal volume of saline. Mouse serum and issue were collected at 24 h after surgery. HE staining was used to observe the histopathological damage in lungs and kidneys. The survival rate was calculated in 7 d after operation; The level of splenic NLRP3 protein was detected by Western blot; IL-1β, IL-18, IL-1α, IL-6, and TNF-α protein level in the serum and lung was measured by ELISA; The proportion of helper T cells (Th)1, Th17, and regulatory T cells (Tregs) in splenic lymphocytes were detected by flow cytometry. Results: Compared with the sham group, the septic mice had inflammatory cell infiltration, oedema and alveolar wall thickening in lungs (P<0.05), and reduction of renal tubular brush border and epithelial cells, glomerular atrophy, diffusely dilatation of the renal tubules with many inflammatory cell infiltration (P<0.05). The expression of NLRP3 in the spleen and the levels of IL-1β, IL-18, IL-1α, IL-6, and TNF-α in the serum and lung in CLP group were higher than that in sham group (P<0.05). Furthermore, compared with sham group, the proportions of CD4+IFN-γ+ Th1 and CD4+IL-17+ Th17 in the spleen were elevated, accompanied by a slight increase in the proportion of CD4+CD25+Foxp3+ Tregs (P<0.05). The 7 d survival rate of septic mice was 30%. Meanwhile, the inflammatory cell infiltration and oedema in the lung in septic mice were attenuated by MCC950 treatment (P<0.05). The glomerular telangiectasia was not obvious, and the dilatation of the renal tubules was decreased in the MCC950 group (P<0.05). Compared with CLP group, the expression of NLRP3 in the spleen in CLP group was down-regulated, and the levels of IL- 1β, IL-18, IL-1α, IL-6, and TNF-α in the serum and lung were reduced (P<0.05). In addition, the proportion of CD4+IFN-γ+ Th1 and CD4+IL-17+ Th17 was decreased, and the proportion of CD4+CD25+Foxp3+ Tregs was more significantly increased than that in CLP group (P<0.05). The 7 d survival rate of septic mice was improved (P<0.05). Conclusion: Inhibition of NLRP3 activation by MCC950 ameliorated organ damage in septic mice, and its effect may depend on inhibiting Th1/Th17 response and promoting Tregs response. 2024 Vol. 54 (3): 190-198 [Abstract] ( 297 ) HTML (1 KB)  PDF (2443 KB)  ( 560 ) 199 The application value of serum amyloid A in the severity and prognosis of viral pneumonia XIONG Xuefang, ZHONG Ying, SUN Debin. DOI: 10.3969/j.issn.2095-9400.2024.03.004 Objective: To investigate the clinical application value of serum amyloid A (SAA) in grading the severity of viral pneumonia and evaluating the prognosis of patients with severe disease. Methods: A t tal of 250 ospitalized patients with viral pneumonia admitted to Lishui Central Hospital from December 2022 to June 2023 ere selected and divided into two groups: non-severe group and severe group. The differences in SAA, CRP,procalcitonin (PCT), total white blood cell (WBC) and neutrophil ratio between the two groups were compared.The ROC curve was used to evaluate the value of each indicator in identifying critically ill patients. According to he survival outcome, patients with severe disease were divided into survival group and non-survival group, and the difference in five indicators between the two groups was compared, and the prognostic value of the different indicators in severe patients was evaluated by ROC curve. Results: The levels of SAA, CRP, PCT, white blood cell count and neutrophil ratio in severe group were significantly higher than those in non-severe group, with statistical significance (P<0.05). The ROC curve analysis showed that the AUC of SAA was 0.869, the cutoff value 226.05 mg/L, and the sensitivity and specificity were 94.1% and 63.7%, respectively. SAA had the best identification efficiency for patients with severe viral pneumonia. Multivariate Logistic regression analysis showed that age ≥88.5 years old, SAA≥226.05 mg/L, central granulocyte ratio ≥88.75% were risk factors for severe viral pneumonia. The levels of SAA and PCT in non-survival group were significantly higher than those in survival group, with significant difference (P<0.05). ROC curve analysis showed that the AUC of SAA was 0.576,the cutoff value 639.35 mg/L, and the sensitivity and specificity were 82.1% and 43.7%, respectively. Conclusion: SAA level can be used as a sensitive index to evaluate the severity of viral pneumonia as it is more effective than CRP, PCT, total white blood cell counts and neutrophil ratio in differentiating non-severe and severe viral pneumonia. It is also has application value in evaluating the prognosis of severe patients. 2024 Vol. 54 (3): 199-204 [Abstract] ( 187 ) HTML (1 KB)  PDF (1519 KB)  ( 527 ) 205 The effect of buccal acupuncture therapy on postoperative analgesia and rehabilitation in patients undergoing rotator cuff repair HUANG Chaoqun, ZHANG Yu, LIN Jiayu, TU Yahui, ZHUANG Jinfei,ZHANG Xutong, LI Jun. DOI: 10.3969/j.issn.2095-9400.2024.03.005 Objective: To observe the effect of buccal acupuncture therapy on postoperative analgesia and rehabilitation in patients with rotator cuff repair. Methods: A total of 140 patients of both sexes, ASA rade IIII,who were scheduled for elective surgery due to rotator cuff injury in our hospital were selected. All patients underwent shoulder arthroscopic rotator cuff repair under unified general anesthesia combined with brachial plexus block. According to the postoperative analgesia protocol, the patients were randomly divided into control group (group A, patient-controlled intravenous analgesia pump used alone) and buccal acupuncture combined with PCIA group (group CA), with 70 cases in each group. The rest and exercise VAS scores at 6, 12, 24 and 48 h post-operation, analgesic consumption and quality of recovery scores (QoR-40 items, ADL score, Barthel index) within 48 h post-operation, ASES score, Constant-Murley shoulder function score and PSQI score three months after surgery were compared between the two groups. Results: Compared with group A, the rest and exercise VAS scores at 6, 12, 24 and 48 h post-operation were significantly decreased (P<0.05), the PCIA analgesia-related adverse reactions significantly decreased (21.0% vs. 40.3%, P<0.05), and QoR-40 items, Barthel index and degree of satisfaction at 48 h were significantly increased (P<0.05). However, there were no significant differences in analgesic dosage, ADL score and Barthel index within 48 hours post-operation, sleep quality (PSQI score), Constant-Murley shoulder function score and ASES score 3 months after surgery between the two groups (P>0.05). Conclusion: Buccal acupuncture can enhance the analgesic effect of PCIA, reduce the adverse reactions to analgesia, and significantly improve the patient’s recovery quality and satisfaction of 48 h after undergoing rotator cuff repair. 2024 Vol. 54 (3): 205-210，216 [Abstract] ( 212 ) HTML (1 KB)  PDF (770 KB)  ( 647 ) 211 Relationship between serum iron metabolism and single nucleotide polymorphism of IRS-2 gene in pregnant women with gestational diabetes mellitus WU Hao, WU Bo, LIN Xuan, XU Yunzhi, HU Su. DOI: 10.3969/j.issn.2095-9400.2024.03.006 Objective: To explore the relationship between single nucleotide polymorphism of IRS-2 gene and serum iron metabolism level in patients with gestational diabetes mellitus (GDM). Methods: 50 GDM patiets admitted to Wenzhou Central Hospital from January 2018 to March 2023 were selected in the observation group,and 50 normal pregnant women were selected in the control group. All of them had their first prenatal examination at 12 weeks of gestation, and the GDM group was diagnosed at 24-28 weeks. Weight, glucose metabolism indicators and iron metabolism indicators were measured in both groups, and single nucleotide polymorphism sequencing of the 1057 site of the IRS-2 gene was performed and its potential relationship with GDM explained. Results:The glucose metabolism and iron metabolism related indicators of the GDM group were significantly higher than those of the normal pregnant women group (P<0.01). Three genotypes were detected at the 1057 site of the IRS-2 gene in both groups, including pure wild (GG), heterozygous (GD), and pure mutant (DD). Compared with the individuals with GG genotype, the GDM risk of individuals with GD (OR=4.19, 95%CI=1.63-10.76, P=0.003)and DD genotypes (OR=10.67, 95%CI=2.96-38.40, P<0.001) were significantly increased. Meanwhile,individuals with GD and DD genotypes exhibited significantly higher iron metabolism levels compared with those with the GG genotype (P<0.05). Conclusion: In pregnant women, the nucleotide polymorphism in 1057 site of IRS-2 gene is significantly correlated with the occurrence of GDM and abnormal accumulation of serum iron metabolism. 2024 Vol. 54 (3): 211-216 [Abstract] ( 145 ) HTML (1 KB)  PDF (1569 KB)  ( 595 ) 217 Efficacy of perceived social support on therapentic outcomes of online Yalom interpersonal process grouppsychotherapy for social anxiety GUO Zeng DOI: 10.3969/j.issn.2095-9400.2024.03.007 Objective: To explore the relationship between perceived social support and social anxiety in the process of online Yalom interpersonal process group therapy and discuss the efficacy of perceived social support on the therapeutic outcomes for social anxiety. Methods: A total of 172 participants from 29 online Yalom interpersonal process groups were selected for 10 sessions of therapy. Pre- and post-treatment assessments were conducted using the interaction anxiousness scale (IAS) and the perceived social support scale (PSSS). Correlation analysis and ridge regression analysis were used to examine the relationship between these variables. Results: The change in therapeutic efficacy (efficacy difference) was significantly positively correlated with changes in PSSS scores and changes in non-family social support scores (P<0.05). Pre-treatment family support (P<0.05), pre-treatment non-family support (P<0.05), and changes in PSSS scores (P<0.01) positively predicted changes in therapeutic efficacy. Changes in family support negatively predicted changes in therapeutic efficacy (P<0.05). Conclusion: The positive correlation between perceived social support and social anxiety remains stable in the process of online Yalom interpersonal process group therapy. Group members with higher pre-treatment family support, higher pre-treatment non-family support, greater improvement in perceived social support, and bigger decrease in family support achieve greater therapeutic benefits on social anxiety. 2024 Vol. 54 (3): 217-221 [Abstract] ( 171 ) HTML (1 KB)  PDF (1456 KB)  ( 405 ) 222 Application of self management scheme based on empowerment theory in targeted combined immunotherapy for liver cancer patients LIN Haiqing, XIAO Yanling, XIE Shaohua, WANG Zhuangshuang, LIN Yiyin,CHEN Yanyan. DOI: 10.3969/j.issn.2095-9400.2024.03.008 Objective: To explore the clinical application effect of self management schemes based on empowerment theory in targeted combined immunotherapy for liver cancer patients. Methods: From April to July 2022, 60 patients with liver cancer who received targeted therapy combined with immunotherapy in the First Affiliated Hospital of Wenzhou Medical University were prospectively selected as the research subjects.According to the envelope method, they were divided as the control group and the observation group, with 30 cases in each. The control group received routine nursing care, while the observation group was supplemented by the self-management scheme based on the empowerment theory. The self-management efficacy and quality of life scores of the two groups were compared one month after discharge. Results: After intervention, the selfefficacy and scores of all dimensions in the observation group were significantly higher than those in the control group (P<0.05). The overall life quality, physical function, role function, cognitive function, emotional function,social function and symptoms like fatigue, nausea and vomiting, dyspnea, insomnia, diarrhea and loss of appetite were significantly improved in the observation group, compared with the control group (P<0.05). Conclusion:The self-management intervention scheme using the empowerment theory can effectively enhance the selfmanagement efficacy of targeted combination immunotherapy patients with liver cancer, improve their selfconfidence and self-management ability to overcome the disease, and improve their quality of life. 2024 Vol. 54 (3): 222-227，232 [Abstract] ( 175 ) HTML (1 KB)  PDF (1495 KB)  ( 415 ) 228 Detection and result analysis of antimicrobial susceptibility in pulmonary tuberculosis patients co-infected with non-fermentative bacteria SUN Haiqiong, PENG Lijun, CAI Long, FANG Tingting, DAI Lingshan, WEI Hui. DOI: 10.3969/j.issn.2095-9400.2024.03.009 Objective: To analyze the detection and drug susceptibility of non-fermenting bacterial infections in pulmonary tuberculosis patients, so as to provide evidence for clinical treatment. Methods: A totl of 394 hospitalized patients with pulmonary tuberculosis and concomitant non-fermenting bacterial infections treated at Hangzhou Red Cross Hospital from January 2018 to December 2022 were selected for the study. Following the standards set by the Clinical and Laboratory Standards Institute (CLI), the detection and drug susceptibility results of isolated non-fermenting bacteria were subjected to statistical analysis. Results: In 394 patients with tuberculosis co-infected with non-fermenting bacteria, a total of 401 strains of non-fermenting bacteria were isolated. The detection rate of non-fermenting bacteria in the Tuberculosis Department was around 30% from 2018 to 2022. The proportion of non-fermenting bacteria in the Tuberculosis Department/the overall hospital showed a yearly increase. Drug susceptibility results revealed that the resistance rate of Pseudomonas aeruginosa to imipenem and meropenem was 14.6% and 12.7% respectively; Acinetobacter baumannii to imipenem and meropenem exhibited the resistance rate of 51.3% and 60.0%, respectively. The resistance rate of Stenotrophomonas maltophilia to both minocycline and ticarcillin/clavulanate was below 10%. The resistance rate of Burkholderia cepacia to meropenem, cefotaxime, minocycline and trimethoprim-sulfamethoxazole ranged from 0% to 15.4%. Conclusion: The ratio of non-fermenting bacteria in the Tuberculosis Department/the overall hospital is increasing annually. Clinicians should pay attention to the detection and drug resistance monitoring of non-fermenting bacterial infections in pulmonary tuberculosis patients. Rational antibiotic use should be guided by drug susceptibility test results combined with clinical considerations. 2024 Vol. 54 (3): 228-232 [Abstract] ( 156 ) HTML (1 KB)  PDF (1448 KB)  ( 490 ) 233 An analysis of the A2B subtype caused by c.539G>C mutation in exon 7 of ABO gene HUANG Ying, HU Siqun, WEN Zhengjing, LING Jiajin. DOI: 10.3969/j.issn.2095-9400.2024.03.010 Objective: To investigate the results of the A2B subtype caused by ABO gene exon 7 c.539G>C mutation. Methods: ABO blood typing was analyzed by the tube agglutination method. Gene sequencing and analysis were performed usingthe Sanger sequencing method. Results: Four probands had A weak B phenotype,which were defined as A2B blood type according to serological characteristics. No anti-A1 antibodies were detected in the serum of probands 1, 3, and 4. Anti-A1 antibodies existed in the serum of proband 2. The genotype of the four probands was ABO*A2.08/ABO*B.01. Sequencing results showed that compared with the A102, A208 had mutations at c.467C>T and c.539G>C positions, resulting in the substitution of the peptide chains P156L and R180P. Conclusion: The c.539G>C mutation in the gene of α-1, 3-N-acetylgalactosamine transferase has produced the A208 phenotype. 2024 Vol. 54 (3): 233-235 [Abstract] ( 147 ) HTML (1 KB)  PDF (1500 KB)  ( 520 ) 244 Progress of Prevotella in bacterial vaginosis and persistent human papilloma virus infection ZHANG Qi,LI Xiushen, WU Xueqing. DOI: 10.3969/j.issn.2095-9400.2024.03.014 Microorganisms associated with bacterial vaginosis (BV) potentially contribute to human papilloma virus (HPV) infection and even persistent infection, while HPV infection can facilitate the progressi n of bacterial vaginosis. Recently, Prevotella has been identified to be intimately tied to persistent HPV infection. prevotella, as a common commensal in the vagina, is a specialized anaerobic bacterium that is difficult to culture and has received scant attention. With more sophisticated technology for genetic sequencing engineering. Prevotella has acquired prominence in gynecological diseases. It has been documented that Prevotella might be an early colonizing flora at the onset of inflammation in the genital tract, which facilitates the invasion of other bacteria and viruses into the genital tract. The authors summarize the research progress in recent years in the role of Prevotella in bacterial vaginosis and persistent HPV infection, with the aim to better understand Prevotella and to provide reference for the prevention and treatment of HPV infection and related diseases. 2024 Vol. 54 (3): 244-248，254 [Abstract] ( 235 ) HTML (1 KB)  PDF (1483 KB)  ( 719 ) 249 Online and offline blended teaching mode based on OBE concept: Taking the course International Trade Practice for example TAO Gang. DOI: 10.3969/j.issn.2095-9400.2024.03.015 Objective: To construct an online and offline blended teaching mode for the course “International Trade Practice” based on the OBE concept, and to explore its practical effectiveness. Methods: Totaly 287 English majors from 6 colleges in Zhejiang Province that offer the course International Trade Practice were selected as the experimental group and 272 as the control group. The experimental group adopted the mixed online and offline teaching mode, while the control group took the traditional teaching mode. The teaching outcome was evaluated by the final exam scores and questionnaire results on the teaching reform mode. Results: After implementing the new teaching mode, it is found that the test scores of the experimental group students were significantly improved compared with the control group (P<0.05), the satisfaction rate of the experimental group with the new teaching mode was significantly higher than that of the control group with the traditional teaching mode (P<0.05) and the students in the experimental group had good feedback on the application of teaching models, the cultivation of practical knowledge and abilities, and the cultivation of independent learning abilities (mean value>6). Conclusion: The new teaching mode based on the OBE concept has achieved better teaching application effect in that it helps to improve students’ autonomous learning ability and promote students to achieve expected learning outcomes. 2024 Vol. 54 (3): 249-254 [Abstract] ( 172 ) HTML (1 KB)  PDF (1435 KB)  ( 551 ) 255 Application of peer feedback in teaching College English Writing to medical students from the perspectiveof New Liberal Arts PAN Yu, DAI Jianchun DOI: 10.3969/j.issn.2095-9400.2024.03.016 Objective: To investigate the application and role of peer feedback within the context of medical student English writing instruction from the perspective of the New Liberal Arts. Methods: A cohort of dental students from Class 1 and 2 (n=60) and a psychiatric anesthesia class (n=56) from the 2020 academic year of Wenzhou Medical University Renji College were selected to form the experimental and control groups, respectively. The experimental group was exposed to a peer feedback approach, whereas the control group engaged in conventional teaching methodologies. An experimental design was implemented, utilizing pre-test and posttest evaluations to measure enhancements in writing proficiency across three dimensions: content accuracy, organizational coherence, and linguistic precision. Thematic analysis was applied to student reflective reports while data and textual analysis were conducted upon teacher questionnaires. Results: The experimental group showed significantly higher post-test writing scores compared to pre-test (t=4.882, P<0.001), and also outperformed the control grop’s post-test scores (t=3.432, P=0.001). Thematic analysis of students’ reflective reports indicated a gradual acknowledgment of peer feedback’s role in enhancing their writing competence, language skills, and medical humanities literacy. Questionnaire data supported the significant contribution of peer feedback to the improvement of students’ language abilities, writing proficiency, humanistic qualities, and autonomous learning capacity in writing. Pearson correlation analysis of the sub-dimensions revealed positive correlations between the effectiveness of peer feedback implementation and the factors of teacher and environment (r=0.528, P=0.006),teacher and assessment strategies (r=0.596, P=0.004), the environment and assessment strategies (r=0.712, P<0.001). Conclusion: Peer feedback functions as a facilitative mediator in the augmentation of linguistic competencies and the literacy of medical humanities among medical students. Teacher involvement, environmental factors, and assessment policies impose a positive impact on peer feedback mechanisms. 2024 Vol. 54 (3): 255-258,封三 [Abstract] ( 184 ) HTML (1 KB)  PDF (1431 KB)  ( 447 )

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