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2024 Vol. 54, No. 2 Published: 2024-02-25   87 The mechanism of nerve growth factor improving ischemic necrosis of random flap in diabetic rats WANG Xiaowu, ZHU Xiandong, WANG Yongqiang, WU Zhixuan, WEN Zhikai, WU Dazhou, CHEN Jicai DOI: 10.3969/j.issn.2095-9400.2024.02.001 Objective: To explore the molecular mechanism of nerve growth factor (NGF) in improving the ischemic necrosis of random skin flap in diabetic rats. Methods: A Wistar rat model of type 1 diabetes was established, who were randomly divided as the DM group (model group) and the DM+ NGF group (experimental group). A modified McFarlane flap measuring 9 cm×3 cm was designed and manufactured for skin flaptransplantation. The survival of the flaps in both groups was observed, and the survival area was measured. Ratflap tissues were collected to investigate the relationship between flap survival and angiogenesis using HE stainingand immunohistochemical staining. Western blot experiments were carried out to study the differences in CD31 and VEGF protein expression between the two groups and the changes in the AGE-RAGE/MAPK-ERK1/2 signaling pathway. Using the established high-glucose human umbilical vein endothelial cell (HUVEC) model, the cell experiment was divided into two groups: cell model group (DM group) and experimented cell group (DM+NGF group). The effects of NGF on cell proliferation, migration, and angiogenesis in HUVECs under high-glucose conditions were analyzed. This was done through CCK-8 proliferation assays, MCM2+ cell immunofluorescence assays, scratch assays, Transwell assays, and angiogenesis assays. The interaction and functional enrichment analysis of NGF with common angiogenesis-related factors such as VEGF, SDF1α, HIF-1α, PDGFA, and TGF-β1 was made using the STRING database. The effect of NGF on their expression was validated through RTqPCR and Western blot. The impact of NGF on HUVECs was further investigated through RNA-seq and Western blot experiments to explore the underlying mechanisms. Results: After successful animal modeling, it was observed that the DM+NGF group had a 10%-20% larger flap survival area compared with the DM group (P<0.01).Immunohistochemical staining of CD31 revealed a higher microvessel density in the DM+NGF group (284.2± 44.76 mm2) compared with the DM group (P<0.01), along with increased expression of CD31 and VEGF (P<0.05). Furthermore, in the flap tissue, the RAGE expression in the AGE-RAGE/MAPK-ERK1/2 signaling pathway decreased (P<0.05), while p-ERK1/2 expression increased in the DM+NGF group (P<0.05). In terms of cellular effects, a high-glucose model with 25 mmol/L was successfully established, and NGF at 100 ng/mL was found to promote HUVEC proliferation (P<0.01). Compared with the DM group, the DM+NGF group exhibited significantly enhanced cell migration and angiogenic capabilities (P<0.01). Moreover, the DM+NGF group showed higher relative expression levels of VEGF, PDGFA, HIF-1α, Arg-1 and bFGF (P<0.05). The KEGG and GO enrichment analysis results were mainly associated with angiogenesis and the AGE-RAGE/ MAPK-ERK1/2 signaling pathway. Conclusion: NGF may promote the angiogenesis of endothelial cells in diabetic rats by affecting the signal pathway of AGE-RAGE/MAPK-ERK1/2, thus improving the ischemic necrosis of the random skin flap. 2024 Vol. 54 (2): 87-98 [Abstract] ( 228 ) HTML (1 KB)  PDF (2851 KB)  ( 674 ) 99 Protein succinylation involved in the suppression of gastric cancer cell proliferation and migration bybutyric acid HUANG Yingpeng, ZHANG Ke, WU Fangquan, SHI Dibang, HAN Qiannian, WANG Fangyan. DOI: 10.3969/j.issn.2095-9400.2024.02.002 Objective: To study the effects of butyrate on the proliferation and metabolism of gastric cancer cells, and explore its potential mechanism. Methods: EdU and Transwell were used to detect the proliferation and migration of gastric cancer cells after 5 mmol/L sodium butyrate treatment for 48 h, and the cells were collected and metabolites were extracted for metabolomic analysis. Then we screened out the significantly enriched metabolic pathways and upregulated metabolites. Western blot and cellular immunofluorescence were used to detect the degree of protein succinylation in gastric cancer cells treated with sodium butyrate. Meanwhile, the mRNA level of SIRT5, SUCLG1, SUCLG2, and SUCLA2 was detected by RT-qPCR. Results: After 48 h of sodium butyrate treatment, the proliferation of gastric cancer cells decreased significantly. The metabolic pathways such as “TCA cycle” and “oxidative phosphorylation” were significantly enriched in the treatment group, and the TCA cycle metabolite L-Malic acid, succinyl-CoA and fumarate were significantly upregulated (all P<0.05).Western blot and immunofluorescence showed that the protein succinylation of gastric cancer cells was activated after sodium butyrate treatment (P<0.05). What’s more, RT-qPCR results confirmed that sodium butyrate treatment increased the mRNA expression of succinyl-CoA synthetase encoding gene, including SUCLG1,SUCLG2, and SUCLA2 (all P<0.05), but did not significantly affect the expression of SIRT5 (P<0.05).Conclusion: Butyrate inhibits the proliferation of gastric cancer cells by activating SUCLs expression and increasing the level of succinyl-CoA to further promote the protein succinylation of gastric cancer cells. 2024 Vol. 54 (2): 99-105 [Abstract] ( 251 ) HTML (1 KB)  PDF (2476 KB)  ( 555 ) 106 Intervention of (Pyr1) Apelin-13 on PI3K/Akt pathway in bupivacaine-induced neonatal rat cardiomyocytearrest LIN Tingting, CHEN Chaoxing, BAO Nana, SHI Kejian, DONG Jiaojiao, LIU Le. DOI: 10.3969/j.issn.2095-9400.2024.02.003 Objective: To explore the role and mechanism of Apelin/APJ system in myocardial toxicity of bupivacaine. Methods: Myocardial cells were extracted from neonatal mice for primary culture. The cells were randomly divided into four groups: blank medium group (DMSO group), bupivacaine 1 mmol/L group (Bup group), (Pyr1) Apelin-13 2 μmol/L group (Apl group) and bupivacaine 1 mmol/L+(Pyr1) Apelin-13 2 μmol/L group (BAp group). After the number of cell basal spontaneous beats were recorded in each group, the drug was administered according to the corresponding group for 6 h. The time after treatment was recorded as T0. The number of cell beats from T0 to T12 was recorded; the morphology of mitochondria in cardiomyocytes of T12 was observed by electron microscope; the content of LHD in cell culture medium by colorimetry; the concentration of Apelin-13 in cardiomyocytes of T12 was detected by ELISA. To detect the expression of APJ, PI3K, Akt, p-PI3K and p-Akt protein in cardiomyocytes of T12 was detected by Western blot. Results: All cardiomyocytes in Bup group stopped beating at T0. Compared with DMSO group, the number of cell beats was significantly decreased (P<0.05); mitochondria were swollen and vacuolated; the content of LDH in culture medium was significantly increased (P<0.05) and the expressions of Apelin-13, APJ, p-PI3K and p-Akt in cardiomyocytes were down-regulated (P<0.05) in Bup group. Compared with Bup group, the number of cell beats was significantly increased (P<0.05); the mitochondrial structure was obviously improved; the content of LDH in culture medium was significantly decreased (P<0.05) and the expression of Apelin-13, APJ, p-PI3K and p-Akt in cardiomyocyteswas up-regulated (P<0.05) in BAp group. Conclusion: (Pyr1) Apelin-13 can reverse bupivacaine-induced cardiomyocyte arrest, which may be related to the activation of PI3K/Akt protein phosphorylation 2024 Vol. 54 (2): 106-111 [Abstract] ( 160 ) HTML (1 KB)  PDF (2305 KB)  ( 534 ) 112 Clinical application of chromosomal microarray analysis in the diagnosis of fetal central nervous systemmalformations XU Chenyang, XIANG Yanbao, ZHOU Lili, LI Huanzheng, LIN Xiaoling, TANG Shaohua. DOI: 10.3969/j.issn.2095-9400.2024.02.004 Objective: To assess chromosomal variations in 232 fetuses diagnosed with central nervous system (CNS) malformations and explore the relationship between genotype and phenotype. Methods: By chromosomal microarray analysis (CMA) and karyotyping, 232 fetuses with CNS malformations diagnosed by prenatal imaging in Wenzhou Central Hospital from 2014 to 2022 were screened for chromosomal variations. The pathogenicity of the detected variations was evaluated and the detection rates across different CNS malformation types was analyzed was analyzed. Results: The results revealed that the detection rate for chromosomal karyotype abnormalities was 9.1% (19/209), while the overall chromosomal abnormality detection rate using CMA was 22.8% (53/232). These abnormalities included one case of polyploid(1/232, 0.4%), 19 cases of aneuploidy(19/232, 8.2%), and 33 other pathogenic or likely pathogenic copy number variations (CNVs) (33/232, 14.2%).The CNVs involved 12 types of microdeletion/microduplication syndromes, uniparental disomy of chromosome 14, and several dosage-sensitive genes, including DLL1, FOXC1, SHH, ZIC2, and CHAMP1. Furthermore, fetuses with non-isolated CNS malformations had a higher rate of pathogenic variations than those with isolated CNS anomalies, and the difference was statistically significant (47.4% vs. 14.9%, P<0.01). Conclusion: In conclusion,CMA is a rapid and reliable technique for detecting CNVs associated with CNS malformations. Research on the correlation between CNS phenotype and genotype is vital for the prevention and control of congenital disabilities and pathogenic mechanisms. 2024 Vol. 54 (2): 112-119 [Abstract] ( 154 ) HTML (1 KB)  PDF (1688 KB)  ( 621 ) 120 The mechanism of hsa_circ_0016600 promoting thyroid cancer cell migration via modulating the miR-149/PTK7 axis ZHANG Yi, LI Feilei, DONG Jianda, ZHU Shaojun, SUN Yihan. DOI: 10.3969/j.issn.2095-9400.2024.02.005 Objective: To investigate the impact of circular RNA hsa_circ_0016600 on the migration of thyroid cancer cells and its mechanisms. Methods: Cancer tissues and normal tissue specimens from 15 thyroid cancer patients treated at the Department of Pate Surgery, the Second Affiliated Hospital of Wenzhou Medical University. Utilize RT-qPCR to assess the expression levels of hsa_circ_0016600, miR-149, and PTK7 in both thyroid cancer tissues and thyroid cell lines. Thyroid cancer cell lines were cultured in vitro, and knockdown experiments were conducted for hsa_circ_0016600 and overexpression experiments for miR-149. Cell proliferation, migration, and invasion capabilities were assessed using CCK-8 assays, transwell assays, and scratch assays. A xenograft subcutaneous tumor model in mice was established to observe the tumorigenic effect of thyroid cancer cells with hsa_circ_0016600 knockdown in vivo. Fluorescence in situ hybridization experiments were conducted to assess the expression level of hsa_circ_0016600 in the xenograft tumor model in nude mice. RNA fluorescence in situ hybridization experiments were performed to determine the cellular localization of hsa_circ_0016600 and miR-149 in thyroid cancer cells. Dual-luciferase reporter gene assays were used to investigate the targeted regulatory relationships between hsa_circ_0016600 and miR-149, as well as iR-149 and PTK7. Results: In human thyroid cancer tissues and cell lines, the expression of hsa_circ_0016600 (P<0.05)and PTK7 (P<0.01) was upregulated, while the expression of miR-149 (P<0.05) downregulated. Knockdown of hsa_circ_0016600 expression inhibited the proliferation (P<0.01), invasion (P<0.01), and migration (P<0.01)capabilities of thyroid cancer cells. Hsa_circ_0016600 could promote the proliferation (P<0.01), migration(P<0.01), and angiogenesis of thyroid cancer cells by targeting miR-149 and upregulating the expression of PTK7 (P<0.01). Additionally, PTK7 was a target gene of miR-149. Conclusion: Hsa_circ_0016600 is highly expressed in thyroid cancer cells and promotes thyroid cancer cell migration by targeting regulation of the miR-149/PTK7 axis. 2024 Vol. 54 (2): 120-127，134 [Abstract] ( 182 ) HTML (1 KB)  PDF (2832 KB)  ( 510 ) 128 FOLR1-targeted deoxyribozyme enhanced the sensitivity of nasopharyngeal carcinoma xenografted tumor to paclitaxel ZHANG Jie, SU Xueping, YANG Zifei, WU Xianmin, LI Heqing DOI: 10.3969/j.issn.2095-9400.2024.02.006 Objective: To investigate whether taxol sensitivity of nasopharyngeal carcinoma (NPC) grafts in nude mice could be enhanced by FOLR1- targeted deoxyribozyme (DrzE). Methods: NPC parent cells—CNE-1 cells and paclitaxel-resistant CNE-1/taxol cells were used for tumor formation experiment in nude mice.Then, the inhibition difference of NPC xenografted tumors was compared after receiving FOLR1-targeted DrzE alone and the combination of DrzE and paclitaxel. RT-qPCR and Western blot were used to detect the expression of FOLR1 in tumors before and after treatment. Results: CNE-1 tumors grew faster and larger than CNE-1/taxol transplanted tumors (P<0.05). Paclitaxel alone could inhibit the growth of CNE-1 transplanted tumor, but had no effect on paclitaxel-resistant tumors. Both CNE-1 and CNE-1/taxol transplanted tumors were inhibited by deoxyribozyme DrzE alone, and the inhibitory effect on the paclitaxel-resistant tumors was more significant(P<0.05). The combination of paclitaxel and deoxyribozyme had more significant inhibitory effect on the transplanted tumors than either drug alone (P<0.05). The expression of FOLR1 in CNE-1/taxol transplanted tumor before treatment was significantly higher than that in CNE-1 (P<0.01). Meanwhile, the expression of FOLR1 in DrzE group and the DrzE-paclitaxel group was significantly lower after treatment than that in the control group(P<0.05). Conclusion: The transfection of deoxyribozyme DrzE targeting FOLR1 in the transplanted tumor of nude mice can reduce the proliferation rate of nasopharyngeal carcinoma and increase the sensitivity of drugresistant transplanted tumor to paclitaxel. 2024 Vol. 54 (2): 128-134 [Abstract] ( 155 ) HTML (1 KB)  PDF (2002 KB)  ( 459 ) 135 Status quo and influencing factors of malnutrition among primary and middle school students in Wenzhou WEN Dandan, GAO Sihai, CHEN Qingqing, CAI Yuanyuan, LIU Qianqian, CHEN Minhe, XIA Ziqi. DOI: 10.3969/j.issn.2095-9400.2024.02.007 Objective: To investigate the current status of malnutrition among primary and middle school students in Wenzhou and explore its influencing factors so as to provid reference for the prevention and control of malnutrition. Methods: A total of 27 449 primary and middle school students in Wenzhou were selected in 2022 using a stratified cluster random sampling method. Physical examination and questionnaire survey were conducted on the participants to assess their nutritional status and analyze the factors influencing malnutrition.Chi-square was performed to analyze malnutrition-related factors and logistic regression was used for multivariate analysis. Results: The prevalence of malnutrition among primary and middle school students in Wenzhou was 7.59%, of which emaciation was dominant, accounting for 96.31%. The prevalence of malnutrition was higher in boys (8.49%) compared with girls (6.51%) (P<0.001). The prevalence of malnutrition in junior middle school students was the lowest (5.82%), and the prevalence in vocational high school students was the highest(10.54%) (P<0.001). Multivariate regression analysis showed that female students (OR=0.657, 95%CI=0.581-0.742), non-anemia (OR=0.734, 95%CI=0.626-0.860), fresh fruit intake frequency at 1 times per day (OR=0.783,95%CI=0.624-0.983) were protective factors against malnutrition. Conclusion: The malnutrition of primary and middle school students in Wenzhou is mainly manifested as emaciation. Gender, school stage, anemia, fresh fruit intake frequency, are the influencing factors of malnutrition. Further attention should be paid to malnutrition in boys and vocational high school students. Such interventions as balanced diet and cultivation of reasonable dietary habits should be carried out. 2024 Vol. 54 (2): 135-140 [Abstract] ( 179 ) HTML (1 KB)  PDF (1449 KB)  ( 666 ) 141 Dynamic monitoring of air quality during cataract phacoemulsification surgery in the clean operating room LI Chunchun, ZHANG Peihua, ZHANG Jingjing, JIANG Ya, PAN Dongdong, CHEN Yanyan DOI: 10.3969/j.issn.2095-9400.2024.02.008 Objective: To study dynamic monitoring of air quality at differenttime during cataract phacoemulsification surgery in the clean operation room so as to provide references for the development of consecutive operation mode. Methods: Air quality detector and air microorganism sampler were used to dynamically evaluate the air quality during cataract phacoemulsification surgery. Results: The air cleanliness during the entire process of cataract phacoemulsification surgery from patient entry to incision closure was in an“N” shape, with the highest concentration of particulate matter in the air at the beginning of anesthesia, showing significant difference (P<0.01). The aerosol concentration in the surgical area of 12 consecutive cataract phacoemulsification operations showed a steady wave trend from the 1st to the 7th, while PM2.5 (0.17 μg/m3) and PM10 (0.27 μg/m3) showed an upward trend from the 8th, with a significant high value (PM2.5 0.3 μg/m3, PM10 0.4 μg/m3, particle number 21.67/L) during the 12th, when the detection of planktonic bacteria was 2 cfu/m3; the aerosol concentration in the surrounding area showed steady fluctuation, which met the dynamic standard.Conclusion: A clean operating room can continuously and effectively maintain the cleanliness of the air in the operating room and the consecutive operation mode is feasible. Considering the clinical manpower arrangement and practical work, it is recommended that 11 or 12 cataract phacoemulsification surgery (4 to 4.5 hours) be consecutively performed to ensure the management efficiency of the operating room. 2024 Vol. 54 (2): 141-145 [Abstract] ( 153 ) HTML (1 KB)  PDF (1712 KB)  ( 552 ) 146 Analysis on the clinical and imaging features of pulmonary embolism with reversed halo sign as the mainCT manifestation HUANG Shengjing, ZHOU Changsheng, ZHANG Lin, LU Lu, ZHANG Xiaoyu. DOI: 10.3969/j.issn.2095-9400.2024.02.009 Objective: To analyze the clinical and imaging features of pulmonary embolism (PE) with reverse halo sign (RHS) as the main CT manifestation so as to better understand the value of this imaging sign in the early diagnosis of PE. Methods: A total of 15 PE inpatients confirmed by CT pulmonary angiography (CTPA)with RHS as the main CT manifestation were collected from the Affiliated Cangnan Hospital of Wenzhou Medical University from January 2016 to April 2023, whose imaging and clinical data were retrospectively analyzed and summarized. Results: Among the 15 PE patients with RHS as the main CT manifestation, the main clinical symptoms were chest pain and dyspnea, but more than half of the patients were complicated by fever, cough and sputum with elevated white blood cells and CRP, in which 11 patients were misdiagnosed as pneumonia and given anti-infection treatment. The results of imaging analysis showed that there were 16 RHS lesions in 15 patients,of which 14 patients had single lesions and 1 patient had 2 RHS lesions. All RHS lesions were located under the pleura of the lower lung, with 10 in the right lower lung and 6 in the left lower lung. RHS lesions were mostly oval (12/16), wedge-shaped (2/16) and hump-like changes (2/16), the long axis of the lesions parallel to the pleura. The RHS center showed low-density with ground-glass shadow surrounded by a solid ring with a polished border; meanwhile,11 patients had a small amount of pleural effusion. After more than 3 months of standardized anticoagulation treatment, all RHS lesions were absorbed, a few changed to linear scar, and pleural effusiondisappeared. Conclusion: Most RHS lesions in PE patients are located under the pleura, especially common on the lower lung field, mainly single-lesion, oval in shape with the long axis parallel to the pleura and often at the same time combined with a small amount of pleural effusion. PE patients with RHS as the main CT manifestation are prone to the misdiagnosis of pneumonia. It should be vigilant for the possibility of PE in patients with the above imaging features. 2024 Vol. 54 (2): 146-150，155 [Abstract] ( 195 ) HTML (1 KB)  PDF (1683 KB)  ( 555 ) 151 The clinical analysis of nasal orbital complications caused by nasal diseases in children SHI Chenjie,CHEN Bobei. DOI: 10.3969/j.issn.2095-9400.2024.02.010 Objective: To explore the clinical characteristics, imaging, diagnosis and treatment of nasal intraorbital complications caused by nasal diseases in children. Methods: Clinical data of 7 children with nasogenic intraorbital complications admitted to the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from 2019 to 2023 were retrospectively analyzed. The clinical characteristics,diagnosis and treatment of the disease were investigated according to relevant medical history, physical examination and imaging data and the literature reviewed. Results: Of the 7 cases, 6 had orbital complications due to acute sinusitis, and 1 case had intraorbital and periorbital lesions due to nasal vestibulitis and nasal furuncle. The clinical manifestations of children in this group included redness and swelling around the eyes, pain, limited movement of eyeballs and eyelids, etc. Among them, 5 children with acute sinusitis had fever with the highest body temperature of 38-40 ℃, some accompanied by cough, headache, nasal congestion and runny nose. CT showed swelling of eyelid and periorbital soft tissue, and clear intraorbital structure in 3 cases and in 4 cases were found blurred edge of the orbital structure, disappearance of the normal structure, limited density or increased diffusion of the orbital space the varied degree of exophthalmosis and the thickened shadow of the internal rectus muscle. After admission, all the patients in this group were given symptomatic treatment such as antibiotics,glucocorticoid anti-inflammatory, proton pump inhibitor to preserve the stomach and cooling fluid, etc. Among them, 1 patient with rhinocorticoid and nasal furuncle was given the above drugs after the operation of nasal furuncle, and 1 patient with nasosinusitis and orbital cellulitis was given nasal endoscopy after conservative treatment failed. All the children were cured and discharged from hospital after 3-13 days of hospitalization.Conclusion: The nasal cavity and sinuses in the human body are adjacent to the orbital structures, and in children,whose bodies are still in the developmental stage, nasal diseases are more likely to invade orbital tissues.Unless diagnosed and treated promptly, it can lead to serious consequences. In principle, early anti-infection and anti-inflammatory therapy and unobstructed drainage should be initiated. If necessary, sinusotomy or orbital decompression surgery may be performed with the collaboration of ophthalmologists. For pediatric patients, the diagnosis and treatment should be focused on the prevention of disease progression, permanent visual loss and ntracranial complications. 2024 Vol. 54 (2): 151-155 [Abstract] ( 252 ) HTML (1 KB)  PDF (1903 KB)  ( 667 ) 158 The research progress of FAPI PET/CT in the diagnosis of gastrointestinal tumors JIN Jingguo, WANG Zhiqiang, SUN Weijun, XU Hui, ZHANG Wanle. DOI: 10.3969/j.issn.2095-9400.2024.02.012 Nuclear medicine radiopharmaceuticals is a type of compound labeled with radioisotopes as a tool for imaging and treatment of specific biology or organs. Isotope labeled fibroblast activation protein inhibitor (FAPI) is a new type of radiopharmaceutical with high sensitivity and specificity, capable of providing highresolution images, accurately detecting and locating tumors, evaluating their growth and metastasis, and detecting therapeutic effects. Widely studied and used in the diagnosis and treatment of various cancers, it can provide detailed information about tumor tissues and help to develop treatment plan for the individuals. In this review, we will introduce the characteristics of FAPI and its application in gastrointestinal tumors, and compare its advantages and disadvantages with 18F-FDG PET/CT in cancer diagnosis to further illustrate the clinical value of FAPI in gastrointestinal tumors. 2024 Vol. 54 (2): 158-164 [Abstract] ( 239 ) HTML (1 KB)  PDF (1473 KB)  ( 575 ) 165 Public values, multidimensional effects and deepening countermeasures: rectification and result applicationafter university political inspection GU Xiaorong. DOI: 10.3969/j.issn.2095-9400.2024.02.013 Strengthening the rectification and result application after university political inspection is required by the deployment of full and rigorous Party self-governance and policy implementation of political inspection work. Since the 18th CPC National Congress, significant results have been achieved in this aspect.However, there remain some problems, including the unsound rectification mechanism, the lack of standardization in handling problem clues and insufficient application of results. Therefore, we should guarantee the basic needs for all parties, enable ourselves with digital intelligence, improve rectification process and emphasize the full responsibility of all levels and parties involved, making joints efforts to do well in “the second half of the article”in the university political inspection. 2024 Vol. 54 (2): 165-168，封三 [Abstract] ( 205 ) HTML (1 KB)  PDF (1444 KB)  ( 605 ) 169 Mendel randomization analysis blended with problem-based learning in the undergraduate periodontologycourse: An exploration of the teaching mode JIA Ruiwei, ZHAO Zheyu, ZHU Xiaodan, CAO Jinfang, WANGHuining, SUN Xiaoyu. DOI: 10.3969/j.issn.2095-9400.2024.02.014 Objective: To explore the teaching mode with Mendel randomization (MR) analysis combined with Problem-Based Learning (PBL), aiming to help students better understand of the association between periodontal diseases and systemic diseases. Methods: Based mainly on the textbook Periodontology,supplemented by the MR Analysis methods, students were guided to explore the association between periodontitis and systemic diseases. Students from two classes of 2019 at Wenzhou Medical University were randomly divided into the experimental group (MR analysis combined with PBL teaching mode, cases=53) and the control group(classroom lectures combined with PBL teaching mode, cases=54). At the end of the course, the two teaching methods were assessed and evaluated by students’ classroom performance scores, examination results, andresearch & innovative thinking abilities. Results: After the teaching reform of “MR analysis combined with PBL teaching”, the theoretical performance of students in the experimental group was better than that of the control group. The average score of the test questions of students in the experimental group was 84.9±4.6, while the average score of the test questions of students in the control group was 79.2±4.3, with statistical difference(P<0.05). Meanwhile, the classroom performance score of students in the experimental group was 96.7±5.7;while the score of the control group was 91.2±4.8, and the difference was statistically significant (P<0.001). In addition, the students in the experimental group had better scientific research and innovative thinking abilitiesthan those in the control group, as evidenced by their participation in more scientific research projects and thesis writing and publication. Conclusion: The teaching method of “MR analysis and PBL teaching” for oral Periodontology deepened students’ understanding and mastery of the correlation between periodontitis and systemic diseases. Through the review of scientific literature, the students had a more in-depth understanding and exploration of the correlation between periodontitis and systemic diseases. Their scientific thinking and team collaboration abilities were greatly improved. 2024 Vol. 54 (2): 169-172，封三 [Abstract] ( 177 ) HTML (1 KB)  PDF (1493 KB)  ( 650 )

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