温州医科大学学报
 
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2023 Vol. 53, No. 7
Published: 2023-07-25
 
 
517 Edaravone Dexborneol facilitates peripheral nerve regeneration through suppressing oxidative damage and autophagy flow
CHEN Jinghao， LOU Chenghao, WEI Shengzhe, LU Yingfeng, YU
DOI: 10.3969/j.issn.2095-9400.2023.07.001
Objective: To investigate the role of Edaravone Dexborneol (C-EDA) in regulating oxidative stress and autophagy flow in peripheral nerve injury (PNI). Methods: PNI model was established by moderate crush injury to the sciatic nerve with a vascular clamp. The rats were randomly divided into Sham group, PNI group, C-EDA group (C-EDA+PNI), and 3MA group [PNI+C-EDA+3MA (3-methyladenine)]. At the 4th week after operation, gait imprinting, neuroelectrophysiological detection, wet weight measurement of gastnemius muscle, HE staining of muscle tissue and NF-200+MBP immunofluorescence staining of nerve tissue were performed in all rats. The levels of oxidative stress-related proteins Nrf2 and HO-1, autophagy related proteins LC3 II/LC3 I and p62 (autophagy substrate protein, reflecting whether autophagy flow was unobstructed), apoptosisrelated proteins Bcl-2 and Bax were detected by Western blot. Results: Compared with the PNI group, C-EDA+ PNI improved the posterior foot deformity, improved nerve conduction function and reduced gastrocnemius atrophy. 3MA reversed the effect of C-EDA on improving neurological function after injury. Compared with PNI group, C-EDA up-regulated the expressions of oxidative stress-related proteins Nrf2, HO-1 and anti-apoptotic protein Bcl-2, and down-regulated the expressions of autophagy substrate protein p62 and pro-apoptotic protein Bax (P<0.05). Conclusion: Our study confirms that C-EDA can unobstruct autophagy flow, playing a role in improving neural structure repair and functional recovery after PNI. Therefore, it is a potentially effective method for the treatment of PNI.
2023 Vol. 53 (7): 517-525 [Abstract] ( 307 ) HTML (1 KB)  PDF (2912 KB)  ( 576 )
526 Melatonin activates Nrf2 to inhibit ferroptosis and improves the survival of random skin flaps
LOUChenghao, LU Yingfeng, WANG Jian.
DOI: 10.3969/j.issn.2095-9400.2023.07.002
Objective: To investigated whether melatonin (MLT) can promote the survival of random flaps ，by reducing ferroptosis in random flaps. Methods: Twenty male C57BL/6 mice aged 6-8 weeks were randomly divided as the control group and MLT group. The flap survival and hemodynamics after 3 and 7 days in both groups were observed. The flap tissue was taken to evaluate edema, and the effect of melatonin on the survival of random flaps and the regulation of ferroptosis was investigated by pathological staining, transmission electron microscope and immunoblotting. In order to explore the role of nuclear factor erythroid 2-related factor 2 (Nrf2) in the inhibition of ferroptosis by melatonin, 45 male C57BL/6 mice aged 6-8 weeks were randomly divided into control group, MLT group, and MLT+ml385 group. Morphological changes were observed and changes in relevant biochemical indicators were detected. Results: Distal flap necrosis occurred in all groups on day 3 and day 7. Compared with the control group, MLT group had the increased flap survival area (P<0.05) and flap blood perfusion (P<0.05). HE staining showed that MLT group had more small blood vessels in the flap, and Masson staining showed that fibres in the dermis were regularly arranged in MLT group. Immunohistochemical stainingshowed that MLT group had more CD34-positive vessels (P<0.05), reduced apoptosis-related proteins (P<0.05) and increased content of antioxidant protein (P<0.05), compared with the control group. Characteristic changes of ferroptosis in the flap tissue were observed by transmission electron microscopy. Tissue lipid peroxide detection, tissue iron content detection, glutathione level and western blot results showed that MLT group up-regulated GSH (P<0.05), decreased tissue iron accumulation (P<0.01), and reduced lipid oxidation level (P<0.05). After the combined use of ml385, the results of western blot showed that compared with the MLT group, Nrf2 (P<0.05),the antioxidant protein HO-1 (P<0.05), the anti-lipid peroxidation protein GPX4 (P<0.05), and the iron-binding protein light chain FTL (P<0.05) were down-regulated in the MLT+ml385 group. Conclusion: This study demonstrates that MLT treatment favors the survival of random skin flaps in mice. Ferroptosis is one of the factors affecting the survival of random skin flaps, and inhibition of ferroptosis can promote the survival of random skin flaps; MLT’s inhibition of ferroptosis depends on the activation of Nrf2 to improve antioxidant capacity, reducelipid peroxidation and promote iron metabolism.
2023 Vol. 53 (7): 526-531，539 [Abstract] ( 343 ) HTML (1 KB)  PDF (2455 KB)  ( 564 )
532 Dynamic changes in the number of retinal ganglion cells and axons after optic nerve crush in mice
SHENBingyan, WANG Min, FANG Yenan, DAI Qin, XIE Qiqi, WU Wencan.
DOI: 10.3969/j.issn.2095-9400.2023.07.003
Objective: To investigate the dynamic changes in the number of retinal ganglion cells (RGCs) and axons at different time points after optic nerve crush in mice, and to further assess the degree of optic nerve injury. Methods: Twenty 1-month-old C57BL/6J mice were randomly divided into four groups: normal control group and experimental groups at 3, 7, and 14 days after optic nerve injury. Fundus photography and optical coherence tomography were performed with the small-animal microscopic imaging system, and the thickness of the total retina and ganglion cell complex (GCC) were measured. Immunofluorescence staining and electron microscopy were used to assess the dynamic changes of the number of RGCs and axons. Results: Three days after optic nerve injury, the thickness of the GCC layer and the number of RGCs in different retinal regions were not significantly different from those in the normal control group (P>0.05), but the axonal survival rate was reduced (P<0.05). The thickness of the GCC layer, the number of RGCs in the peripapillary region and the lateral region of the retina, and the axonal survival rate on day 7 post-injury were reduced, compared with normal control group (P<0.05). The thickness of the GCC layer, the number of RGCs in the per retinal region, and the axonal survival rate on day 14 post-injury decreased significantly compared with other groups (P<0.05). Besides, the thickness of the GCC layer, the number of RGCs, and the axonal survival rate were all positively correlated (P<0.001). Conclusion: During the early stage of optic nerve injury (3 days post-injury), the survival rate of RGCs and axons is high. However, the survival rate of RGCs and axons gradually decreases during the middle and late stages of optic nerve injury (7 to 14 days post-injury). Therefore, prompt intervention during the early stage of optic nerve injury may be helpful to prevent further deterioration of visual function.
2023 Vol. 53 (7): 532-539 [Abstract] ( 244 ) HTML (1 KB)  PDF (2112 KB)  ( 655 )
540 The effect and mechanism of carnosine on glioma cell proliferation under normoxic and hypoxic conditions
DUAN Rongqing, LI Liucen, TANG Qi, MEI Lei, SHEN Yao.
DOI: 10.3969/j.issn.2095-9400.2023.07.004
Objective: To study the effect of carnosine on the proliferation of glioma cells and to explore its mechanism. Methods: Cell samples were divided into normoxia group (21% O2), normoxia carnosine group,  hypoxia group (1% O2), hypoxia carnosine group. Under normal oxygen and hypoxia treatment, U251 and U87 cells were treated with 25 mmol/L and 50 mmol/L carnosine, and the changes of glioma cell proliferation were detected by MTT method. The clonal formation experiment was used to detect the change of clonal formation ability of glioma cells. Annexin V-FITC/PI apoptosis was detected by Annexin V-FITC/PI apoptosis assay kit and flow cytometry. Non-targeted metabolomics was used to analyze the metabolic changes of gliomas; LC-MS method was used to detect the change of methionine content in glioma cells. The Western blot method was used to detect the expression levels of c-Myc and LAT1 proteins. C-Myc and LAT1 mRNA levels were determined by RT-qPCR. Results: After carnosine treatment, carnosine can significantly inhibit the proliferation and clonal formation ability of glioma cells under normal oxygen and hypoxic conditions (P<0.05). Carnosine can promote apoptosis in glioma cells (P<0.05). Carnosine had an effect on the amino acid metabolism of U251 cells, which
reduced the content of lysine, methionine and arginine (P<0.05). After carnosine treatment of U251 cells, both c- Myc and LAT1 expression were downregulated (P<0.05). Conclusion: Under normoxic and hypoxic conditions, carnosine can inhibit the proliferation of glioma cells and induce their apoptosis, which may be achieved byinhibiting the c-Myc/LAT1 axis.
2023 Vol. 53 (7): 540-547 [Abstract] ( 233 ) HTML (1 KB)  PDF (2117 KB)  ( 693 )
548 Virtual screening model for fibroblast growth factor receptors kinase inhibitors based on machine learning
DING Juntao, LIU Bo, WU Jianzhang, LI Wulan.
DOI: 10.3969/j.issn.2095-9400.2023.07.005
Objective: To construct an efficient virtual screening model to filter fibroblast growth factor receptors (FGFR) kinase inhibitors based on machine learning. Methods: FGFR kinase inhibitors from the public dataset BindingDB were collected; RDkit was used to calculate molecular descriptors to characterize compound molecules for data input. Two machine learning algorithms (random forest and support vector machine) were used to establish a virtual screening model, and four indicators [accuracy, precision, recall and area under curve (AUC)] were used to evaluate the model. Preliminary screening of 13 million compounds using a random forest
model; Subsequently, Autodock Vina and Glide methods were used to further screen FGFR1 kinase inhibitors; Molecular dynamics simulation was used to analyze the compounds obtained through virtual screening. Results: The constructed random forest model and support vector machines model had good performance in terms of accuracy, precision, recall and AUC. The accuracy and AUC of the random forest model reached 0.878 and 0.952 respectively, which could be used as a virtual screening model for FGFR kinase inhibitors. When the random forest model was used in the high-throughput virtual screening to obtain highly active lead compound, the molecular docking and molecular dynamics analysis of the three selected optimal compounds with FGFR1 kinase showed that there was a high similarity with the positive drug AZD4547 in terms of hydrogen bonding, binding free energy,and hydrophobic effect. The LEU21, VAL29 and ALA49 residues of FGFR1 are important residues for small  molecule drugs to maintain stable binding. Conclusion: This study provides a virtual screening model for FGFR kinase inhibitors based on machine learning, which can be used for efficient screening of large-scale small
molecule compound libraries.
2023 Vol. 53 (7): 548-555，564 [Abstract] ( 346 ) HTML (1 KB)  PDF (2433 KB)  ( 654 )
556 CT-guided 125 I seeds implantation combined with chemotherapy in treatment of lung metastatic tumors from colorectal cancer: an analysis of clinical efficacy and prognostic tactors
LI Yanjun, CHEN Minjiang,TU Jianfei, PAN Yiying, WANG Junhe, JI Jiansong.
DOI: 10.3969/j.issn.2095-9400.2023.07.006
Objective: To investigate the feasibility of percutaneous 125I implantation guided by computed tomography combined with chemotherapy in the treatment of lung metastases of colorectal cancer, and to evaluate its clinical efficacy and prognostic factors. Methods: Retrospective analysis was performed on 97 patients with lung metastatic tumor of colorectal cancer admitted to the Fifth Affiliated Hospital of Wenzhou Medical University from January 1, 2016 to December 31, 2020. According to different treatment, they were divided into combination treatment group (n=43) and control group (n=54). The combination treatment group received percutaneous 125I seed implantation combined with chemotherapy, while the control group was treated with conventional chemotherapy alone. With general clinical data collected, we compared the efficacy and adverse reactions of the two groups. The last follow-up was January 1, 2022. Results: The target objective response rate (ORR) and the
disease control rate (DCR) of the combined treatment group was 72.1% and 93.0%, respectively, with statistical difference (P<0.05). The survival time analysis of the two groups showed the median PFS and OS of the combined treatment group were significantly better, up to 13 (7.0, 16.0) months and 20.0 (14.0, 40.0) months, with significant difference (P<0.05). After 3 months of treatment, CA19-9 in the combined treatment group waslower than the control group, with statistical significance (P<0.05). There was no significant difference in the rate of adverse reactions after chemotherapy between the two groups (P>0.05). All adverse reactions in both groups
were 1-2 mild adverse reactions. Cox proportional risk regression model analysis showed that after particle implantation (HR=0.35), the TNM stage of the primary tumor was stage I-II (HR=2.59), DFI>12 months (HR=1.71) and ECOG score 0-1 (HR=2.18) patients had longer PFS; Patients with primary TNM stage I-II (HR=2.08), no more than 3 metastases (HR=2.27), and metastatic sites in a single lung (HR=2.61, 3.30) had longer OS. Conclusion: CT-guided 125I seed implantation combined with chemotherapy can be used as the preferred treatment for its effectiveness in the treatment of colorectal cancer lung metastases. The TNM stage of the primary tumor is found to be an independent risk factor affecting PFS and OS, with the ECOG score and DFI of patients related to PFS, and the preoperative CA19-9 level, the number and site of metastases related to OS. Therefore, patients with primary colorectal cancer stage TNM I and II benefit the most from 125I seed implantation combined with chemotherapy.
2023 Vol. 53 (7): 556-564 [Abstract] ( 250 ) HTML (1 KB)  PDF (1452 KB)  ( 457 )
565 Preparation and characterization of an ultrasound-controlled polypeptide nanoparticle drug release system
ZHANG Yu, YIN Hao, WANG Qinyang.
DOI: 10.3969/j.issn.2095-9400.2023.07.007
Objective: To design and prepare an ultrasound-controlled polypeptide nanoparticle drug release system, and to characterize its drug inclusion rate and release rate after ultrasound. Methods: By adding 5K, 10K and 20K polyethylene glycol amine initiators and polypeptides with polymerization degree 20, polymerization degree 30 and polymerization degree 40, respectively, eight different types of polypeptide nanoparticle drug release systems were synthesized, including 5K initiator polymerization degree of 20[5K(1:20)], 5K(1:30), 5K(1:40), 10K(1:20), 10K(1:30), 10K(1:40), 20K(1:30) and 20K(1:40). Considering that the side chain of polypeptide can be modified, ultrasound sensitized agent was added, and the modification of ultrasound sensitized agent on polypeptide was characterized by means of UV-visible light. Firefly luciferase has a specific response to ATP, so it can characterize the ATP-coated polypeptide nanoparticle drug release system with ultrasound control, including the inclusion rate, particle size after inclusion, ultrasound particle size change and the release rate of ultrasound response. In addition, the effect of different ultrasound power and ultrasound time on ATP release rate were analyzed. Results: Of 8 kinds of ultrasound-controlled polypeptide nanoparticle drug release systems, the one with 5K(1:40) was insoluble, while the other 7 kinds confirmed the modification of ultrasound sensitized agent through UV-visible light, with good solubility, all of which could realize the inclusion of ATP. In addition, all of them were ultrasound responsive with varying degrees of particle size reduction and ATP release after ultrasound. Among them, the one with 10K initiator polymerization degree of 30 after ultrasound had the highest ATP release rate of 15%, showing statistical difference compared with the ones with 5K(1:20), 5K(1:30), 10K(1:20), 10K (1:40), 20K(1:30) and 20K(1:40). The polypeptide nanoparticle drug release system with 10K(1:30) had good drug loading stability. Without ultrasound treatment, there was no significant difference in ATP content and particle size within 24 hours compared with 0 hour. The polypeptide nanoparticle drug release system with 10K(1:30) was influenced by ultrasound power and ultrasound time. Under the same ultrasound time, the ATP release rate gradually increased with the ultrasound power. There was statistical difference between the 1.0 Wcm-2 group and the 0.5 Wcm-2 group, between the 1.5 Wcm-2 group and the 1.0 Wcm-2 group, between theb 2.5 Wcm-2 group and the 2.0 Wcm-2 group. However, cell viability gradually decreased with the ultrasound power, with statistical difference between the 1.5 Wcm-2 group and the 1.0 Wcm-2 group, between the 2.0 Wcm-2 group and the 1.5 Wcm-2 group, between the 2.5 Wcm-2 group and the 2.0 Wcm-2 group. Under the same ultrasound power, the ATP release rate of the polypeptide nanoparticle drug release system also increased gradually with the ultrasound time, showing statistical difference between the 10 min group and the 1 to 8 min group. Conclusion: The results of a series of screening experiments showed that the ultrasound-controlled polypeptide nanoparticle drug release system with 10K(1:30) has the best ultrasonic response, with 23% inclusion rate and a particle size of 129 nm. The particle size changes by 10 nm after ultrasound, and the ultrasound-controlled release efficiency can reach 15%. When the ultrasound power is 1.0 Wcm-2 and the ultrasound time is 10 min, it has good drug release efficiency and better safety with minimal cell damage.
2023 Vol. 53 (7): 565-573 [Abstract] ( 334 ) HTML (1 KB)  PDF (1741 KB)  ( 666 )
574 Aerobic exercise ameliorates pressure overload-induced cardiac remodeling by inhibiting TAK1-MAPK signaling pathway
CHEN Qiaoying, JIANG Yi’na, GUAN Xuan, ZENG Jingjing, WU Lianpin,LI Lei, HU Yunliang
DOI: 10.3969/j.issn.2095-9400.2023.07.008

Objective: To investigate the effect of aerobic exercise on pressure overload-induced myocardial remodeling and its mechanism. Methods: Male C57BL/6J mice aged 6-8 weeks were randomly allocated into sham+control (Sham+CON) group, sham+swimming (Sham+SWIM) group, TAC+control (TAC+CON) group, and TAC+swimming (TAC+SWIM) group. One week after operation, mice in the swimming groups commenced aerobic exercise training. After the aerobic training, echocardiography was employed to assess the cardiac function of the mice, while HE staining, Masson staining, and TUNEL staining were utilized to examine and analyze the cross-sectional area of myocardial cells, myocardial interstitial fibrosis, and apoptosis of myocardial cells. The protein expression levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), Collagen I and III,Bax, Bcl-2, phosphorylated transforming growth factor-β-activated kinase 1 (p-TAK1), phosphorylated c-Jun N-terminal kinase 1/2 (p-JNK1/2), phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), and phosphorylated P38 (p-P38) were investigated through western blot analysis. Results: Echocardiographic assessment revealed that the TAC+CON group had significantly lower left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF) (all P<0.05), compared with the Sham+CON group. Aerobic exercise could significantly improve the LVFS and LVEF of TAC mice. Hematoxylin and eosin (HE) staining, Masson’s trichrome staining, and TUNEL staining demonstrated that aerobic exercise notably diminished the cross-sectional area of cardiomyocytes, myocardial interstitial fibrosis, and cardiomyocyte apoptosis in TAC mice (all P<0.05). Western blot analysis indicated that compared with the Sham+CON group, protein expression levels of ANP, BNP, Bax, Collagen I and Collagen III were increased, Bcl-2 expression was decreased, and the  phosphorylation levels of TAK1, JNK1/2, ERK1/2, and P38 proteins were elevated in the TAC+CON group (all P<0.05). After aerobic exercise, protein expression levels of ANP, BNP, Bax, Collagen I and Collagen III were decreased, Bcl-2 expression was increased, and phosphorylation levels of TAK1, JNK1/2, ERK1/2, and P38 proteins were decreased (all P<0.05). Conclusion: Aerobic exercise can enhance cardiac function in TAC mice by suppressing the TAK1-MAPK signaling pathway, thus ameliorating myocardial interstitial fibrosis and mitigating pressure overload-induced cardiomyocyte apoptosis.
2023 Vol. 53 (7): 574-581 [Abstract] ( 240 ) HTML (1 KB)  PDF (2856 KB)  ( 625 )
582 The status quo and influencing factors of school adaptation of childhood leukemia survivors’ back to school
OU Yunxia1, YU Yahui, HU Jiqi, ZHENG Xiaofen, ZHANG Huihui, WANG Runping, ZHANG Chunmei.
DOI: 10.3969/j.issn.2095-9400.2023.07.009
Objective: To investigate the status quo and influencing factors of school adaptation in childhood leukemia survivors’ return to school. Methods: From March 2022 to September 2022, 102 cases of childhood leukemia survivors were selected using convenience sampling from the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University and the Children’s Hospital of Zhejiang University School of Medicine. The general data questionnaire and School Adaptation Scale were used for the investigation. Multivariate linear regression analysis was used to study the influencing factors of school adaptation in childhood leukemia survivors back to school. Results: The total score of school adaptation in childhood leukemia survivors returning to school was (106.18±14.53). The scores of the dimensions were learning adaptation (29.75±4.34), behavioral adaptation (27.67±4.85), relational adaptation (30.01±3.57), and emotional adaptation (18.75±5.58). The results of multiple linear regression analysis showed that age, length of school absence, physical decline, and change in appearance and image were the main factors affecting school adaptation of childhood leukemia survivors to return to school (P<0.05), which could explain 57% of the total variation. Conclusion: The school adaptation of childhood leukemia survivors is at low level, which needs active coordination among families, hospitals and schools. To promote their school adaptation, special attention should be paid to childhood leukemia survivors aged 13-16, who have suspended from school for 2-3 years and have experienced physical decline as well as image change.
2023 Vol. 53 (7): 582-587 [Abstract] ( 223 ) HTML (1 KB)  PDF (1223 KB)  ( 669 )
588 Establishment of machine learning model for predicting bone metastasis in patients with lung adenosquamous carcinoma
ZHU Yinghao, WANG Shiqi, ZHANG Wei, LIU Yu.
DOI: 10.3969/j.issn.2095-9400.2023.07.010
Objective: To explore the value of machine learning algorithm in the prediction model of lung adeno-squamous cell carcinoma bone metastasis. Methods: Data of patients with lung adeno-squamous cell carcinoma were obtained from the Surveillance, Epidemiology and End Results Database (SEER) and the First  Affiliated Hospital of Wenzhou Medical University between January 2017 and December 2021. In order to build the prediction model, six algorithms including random forest (RF), support vector machine (SVM), eXtreme Gradient Boosting (XGBoost), Gradient Boosting Machine (GBM), Multi-Layer Perceptron (MLP) and KNearest  Neighbor (kNN) were applied. ROC curve was used to evaluate the prediction capability of these models. Results: Medical records of 1,919 eligible patients were obtained from the SEER database, and 51 obtained from the First Affiliated Hospital of Wenzhou Medical University. Machine learning model results showed that lung adenosquamous metastasis to organs other than bone and lymph node metastasis were the most important predictors of bone metastasis. The attributes of machine learning model based on XGBoost algorithm were more accurate and efficient. Conclusion: Bone metastasis of lung adenosquamous cell carcinoma leads to poor prognosis. The predictive model based on machine learning can accurately predict the possibility of bone metastasis in patients with lung adenosquamous cell at early stage, which is of great significance in clinical decision-making.
2023 Vol. 53 (7): 588-594，封三 [Abstract] ( 359 ) HTML (1 KB)  PDF (1502 KB)  ( 682 )
595 Latent profile analysis of community residents’ risk perception in public health emergencies
ZHANG Yao,SUN Hongyu, TAO Jingrui, XU Huan, DONG Chaoqun.
DOI: 10.3969/j.issn.2095-9400.2023.07.011
Objective: To explore the potential categories of community residents’ risk perception in public health emergencies and analyze the influencing factors of different categories. Methods: A cross-sectional study was designed to recruit 7 844 residents from 30 provinces in China from March to May 2022 by convenient sampling. The general characteristics, COVID-19 Risk Perception Scale, Simplified Coping Style Questionnaire,and Psychological Questionnaires for Emergent Events of Public Health were collected. Latent profile analysis was used to explore the classification of the risk perception of community residents, and multinomial logistic regression analysis was used to compare the characteristics of each latent category and to investigate their influencing factors. Results: The risk perception of community residents could be divided into three categories: low risk-perception (23.9%), high risk-perception (29.3%), and high risk-perception with low-susceptibility in general (46.8%). The results of multinomial logistic regression showed that compared with the low risk-perception group, residents who were older (OR=1.021, P<0.001), female (OR=1.283, P=0.008), rural (OR=1.198, P=0.014), who had been isolated (OR=1.277, P=0.031), had≤1 vaccination (OR=1.614, P=0.036), and had higher scores of negative coping (OR=1.420, P<0.001), depression (OR=1.324, P=0.001), fear (OR=2.579, P<0.001), and suspicion of illness (OR=1.421, P<0.001) tended to be in the high risk-perception group, while residents with technical secondary or high school degrees (OR=0.733, P=0.045), who had higher scores of positive coping (OR=0.662, P<0.001), and obsessive-compulsive-anxiety (OR=0.786, P=0.043) were less likely to be in the high risk-perception group. Compared with the low risk- perception group, residents who were female (OR=1.382, P<0.001), living in the rural area (OR=1.297, P<0.001), who had higher scores of negative coping (OR=1.202, P<0.001), neurosis (OR=1.189, P=0.023), and fear (OR=2.458, P<0.001) tended to be in the group of lowsusceptibility high -perception, while residents who were married (OR=0.798, P=0.013), who had been engaged in epidemic-related activities (OR=0.862, P=0.015), had higher scores of positive coping (OR=0.750, P<0.001), and obsessive-compulsive-anxiety (OR=0.653, P<0.001) were less likely to be in the low-susceptibility highperception group. Conclusion: There is heterogeneity in the risk perception of community residents in public health emergencies, and the residents’ risk perception is related to coping style and mental health status. Community medical staff should carry out targeted interventions according to the characteristics of different categories.
2023 Vol. 53 (7): 595-602，封三 [Abstract] ( 282 ) HTML (1 KB)  PDF (1290 KB)  ( 631 )
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