温州医科大学学报
 
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2022 Vol. 52, No. 6
Published: 2022-06-25
 
 
431 Treadmill exercise inhibited WNK2 to improve cardiac remodeling in diabetic cardiomyopathy
WANG Ting, CAI Xueli, LI Haiyan, HUANG Zhouqing, WANG Yonghua
DOI: 10.3969/j.issn.2095-9400.2022.06.001
Objective: To investigate the effect of exercise training on preventing diabetic cardiomyopathy and to determine the role of WNK2 in high-glucose induced cardiomyocytes. Methods: Male db/db mice and C57BL/6 mice (as control) were fed a regular diet for 10 weeks, then began running exercise or remained sedentary
for 10 weeks. After that, cardiac function was assessed by echocardiography, and heart tissues were collected for further measurements including Hematoxylin-Eosin(HE) staining, Masson’s staining, wheat germ agglutinin (WGA) staining, real-time quantitative polymerase chain reaction (RT-qPCR) and Western blot. In addition, after
pretreatment with WNK463 (a WNK2 inhibitor) in high glucose-induced H9C2 cardiomyocytes, the indicators of hypertrophy, fibrosis of cells, collagen type I (COL-I) and transforming growth factor β (TGF-β) were tested.Results: Compared with the control, the heart of db/db mice showed cardiac hypertrophy and fibrosis, reduced
cardiac function and increased WNK2 levels, with statistical difference (P<0.05). After 10 weeks of treadmill exercise, cardiac hypertrophy and collagen deposition were reversed, cardiac systolic function was improved, and WNK2 expression was decreased, showing significant difference (P<0.05). Mechanistically, WNK2 inhibition
prevented the expression of myosin heavy chain (MyHC) and atrial natriuretic peptide(ANP), as well as fibrosis reflected by matrix metalloprotein-9 (MMP9), collagen-I (COL-I) and transforming growth factor-β (TGF-β) in high glucose-stimulated cardiomyocytes, indicating that the suppression of WNK2 could reduce hypertrophy and fibrosis of cells. Conclusion: Running exercise limits cardiac hypertrophy, fibrosis and reverses cardiac dysfunction in diabetic cardiomyopathy by inhibiting WNK2 upregulation.
2022 Vol. 52 (6): 431-439,445 [Abstract] ( 467 ) HTML (1 KB)  PDF (2798 KB)  ( 687 )
440 Regulator of G protein signaling 13 plays an inhibitory role in the progression of colorectal cancer via regulating β-catenin pathway
XIANG Youqun, FANG Guan, JIN Yixiang, YANG Kai, PAN Yifei
DOI: 10.3969/j.issn.2095-9400.2022.06.002
Objective: To explore the role of regulator of G protein signaling 13 (RGS13) in the progression of colorectal cancer (CRC). Methods: The expression of RGS13 in CRC tissues and cells was analyzed at the mRNA level using the TCGA database and real-time quantitative polymerase chain reaction (qPCR). Further analysis at the protein level was performed using immunohistochemistry (IHC) and western blot. The effect of RGS13 on the proliferation, migration and invasion of CRC cells was detected by ATP cell viability assay,soft agar colony formation assay and cell migration and invasion assay. The downstream molecular mechanism was explored by western blot, RT-qPCR and other experiments. Results: The expression of RGS13 was low in CRC tissues and cell lines (P<0.01). The lower the expression of RGS13, the shorter the disease-free survival of patients (P=0.017). Down-regulation of RGS13 could significantly promote the proliferation, migration and invasion of CRC cells (P<0.01), indicating that RGS13 played a tumor suppressor role in the progression of CRC.Mechanistically, RGS13 inhibited CRC by down-regulating the protein level of β-catenin in the Wnt/β-catenin signaling pathway, thereby reducing the expression levels of oncogenes c-Myc, MMP7 and CCND1 (P<0.01).Conclusion: RGS13 may play an important role in inhibiting CRC progression by down-regulating β-catenin.
2022 Vol. 52 (6): 440-445 [Abstract] ( 606 ) HTML (1 KB)  PDF (1908 KB)  ( 727 )
446 Lipoxin A4 regulates epithelial-mesenchymal transition in epithelial cell through inhibiting activation of ERK1/2 pathway
LUO Yacan, WU Zhenjie, JIN Minli, LOU Lejing, YANG Song, CAI Jihao, CAIChang
DOI: 10.3969/j.issn.2095-9400.2022.06.003
Objective: To investigate the effects of lipoxin A4 (LXA4) on transforming growth factor β1 (TGF-β1)-induced epithelial-mesenchymal transition (EMT) in BEAS-2B (human normal bronchial epithelial cell) and ovalbumin (OVA)-induced airway remodeling in asthmatic mice and its possible mechanism. Methods: The BALB/c mice were randomly divided into 4 groups: control group, OVA group, LXA4 group and OVA+ LXA4 group. The pathological changes of lung tissues were observed by HE and PAS staining. BEAS-2B cells were divided into 6 groups: control group, interleukin (IL)-4 group, IL-13 group, TGF-β1 group, IL-4/IL-13/TGF-β1 co-stimulation group and LXA4 pretreatment group. The mRNA expression of α-smooth muscle actin (α-SMA), E-cadherin were detected by quantitative real time PCR. α-SMA, E-cadherin, ERK1/2 and pERK1/2 protein expressions were measured by Western blot. Results: Compared with the control group, the lungs in the model group exhibited observable lung histopathologic changes, including alveolar wall thickening, interstitial edema, inflammatory infiltration, hemorrhage, and lung tissue destruction. In addition, mice in the OVA group showed strong AB/PAS-positive staining in their bronchial epithelial tissues, which indicated goblet cell hyperplasia and mucus production, but LXA4 significantly improved the above-mentioned pathological changes.In BEAS-2B, compared with control group, cells treated with TGF-β1 for 72 hours became spindle-shaped, loosely attached, and cell intercellular spaces-enlarged. Cells stimulated by a combination of TGF-β1, IL-4 and IL-13 exhibited increased morphological alterations, compared with the TGF-β1 group. Compared with controlgroup, the expression of α-SMA mRNA and protein and pERK protein increased, while expression of E-cadherinmRNA and protein decreased in TGF-β1 group. α-SMA and pERK increased while E-cadherin decreased in theTGF-β1/IL-4/IL-13 co-stimulation group more significantly than the TGF-β1 group. Meanwhile, LXA4 inhibitedthe increase of TGF-β1, IL-4, IL-13 co-stimulation-induced α-SMA and the decrease of E-cadherin, and reducedERK phosphorylation in BEAS 2B cells. Conclusion: LXA4 could inhibit OVA-induced airway remodeling inasthma mouse models. Th2 cytokines IL-4 and IL-13 are condusive to EMT in BEAS-2B cells. LXA4 inhibitsEMT in airway epithelial cells, which may depend on the blocking of ERK1/2 pathway phosphorylation.
2022 Vol. 52 (6): 446-452 [Abstract] ( 463 ) HTML (1 KB)  PDF (2176 KB)  ( 567 )
453 Effects of limb ischemic preconditioning / postconditioning on myocardial IL-6 and cardiac function in septic mice
CHEN Haili, GONG Jiahong, LI Xuqing, LI Shengcun
DOI: 10.3969/j.issn.2095-9400.2022.06.004
Objective: To investigate the effects of limb ischemic preconditioning/postconditioning on myocarditis and cardiac function in sepsis mice. Methods: Sepsis mouse model was induced by intraperitoneal injection of lipopolysaccharide (LPS) 6 mg/kg. Sepsis mice were subjected to single and bilateral leg ischemic preconditioning or post adaptation intervention, or combined with ischemic preconditioning and post adaptation intervention. Proinflammatory factor interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor (TNF-α), Apelin and Atrogin-1 mRNA expression were detected by qRT-PCR. The cardiac functions of mice,including systolic function index ejection fraction (EF), transient shortening rate (FS) and diastolic function index E/A ratio, were measured by small animal ultrasound. Results: Both preconditioning and postconditioning of unilateral leg ischemia could reduce the expression of IL-6 mRNA in myocardium of septic mice (P<0.05).The combination of preconditioning and postconditioning decreased the expression of IL-6 more than ischemic preconditioning/postconditioning alone (P<0.05), but compared with LPS group, the superposition of preconditioning at an interval of 2 h further increased the expression of IL-6 (P<0.05). LPS increased IL-1β,TNF-α and atrophic gene Atrogin-1 but decreased apelin gene, while ischemic preconditioning could enhance cardiac function and reduce IL-1 β and TNF-α in septic mice, but did not change the expression of Atrogin-1 and Apelin (P>0.05). Conclusion: Ischemic preconditioning and postconditioning can effectively alleviate the high expression of IL-6 mRNA in myocardium induced by LPS, but postconditioning has no cumulative effect. IL-6 may be an important molecule involved in ischemic preconditioning to improve cardiac function in septic mice.
2022 Vol. 52 (6): 453-458 [Abstract] ( 488 ) HTML (1 KB)  PDF (1692 KB)  ( 555 )
459 The role of dosage-sensitive sex reversal-adrenal hypoplasia congenita region on the X chromosome 1 in autophagy and proliferation on pituitary adenoma cells
CHEN Xianbin, HU Qiongshuang, LYU Fangfang, LU Jianglong, LI Qun
DOI: 10.3969/j.issn.2095-9400.2022.06.005

Objective: To investigate the role of the dosage-sensitive sex reversal-adrenal hypoplasia congenita region on the X chromosome 1 (DAX-1) in autophagy and proliferation of pituitary tumor cells. Methods: The differential gene group between pituitary tumors and normal pituitaries was screened by Microarray and the expression difference of DAX-1 between invasive pituitary tumor and non-invasive pituitary tumor was analyzed by Pubmed database. The expression of DAX-1 pair was knocked down by small interfering RNAs (siRNA), and the effect of DAX-1 on MMQ cells proliferation was detected by CCK-8 and colony formation assays. The expression of autophagy related genes ATG5, ATG7 and ATG12 was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Western blot and immunofluorescence experiments were performed to detect the effect of DAX-1 on the expression of LC3. Finally, xenograft experiments in nude mice was made to
observe the effect of DAX-1 knockdown on the growth and autophagy of pituitary tumor cells. Results: Compared with normal pituitary tumors, the DAX-1 gene expression was significantly down-regulated in pituitary tumors,and was lower in invasive and non-invasive pituitary tumors (P<0.05). Knockdown DAX-1 could promote MMQ cells proliferation, while overexpression of DAX-1 inhibited proliferation of MMQ cells and promoted drug sensitivity of cabergoline and bromocriptine (P<0.05). In addition, knocking-down DAX-1 could inhibit the autophagy level in MMQ cells. It was proved that knocking down DAX-1 promoted tumor growth in vivo (P<0.05). Conclusion: The DAX-1 expression is decreased in pituitary tumors, and the decreased DAX-1 promotes the development of pituitary tumors. DAX-1 may be a potential therapeutic target for pituitary tumors.
2022 Vol. 52 (6): 459-464,471 [Abstract] ( 604 ) HTML (1 KB)  PDF (2156 KB)  ( 678 )
465 The effect of integrated behavioral model-based health education on the caregivers of autistic children
LIU Yiwei, WU Yudan, WANG Chenjun, LI Xia, YE Haijing, CHEN Xiang, DONG Chaoqun
DOI: 10.3969/j.issn.2095-9400.2022.06.006
Objective: To explore the effect of integrated behavioral model-based health education on family function, autism caregivers’ parenting self-efficacy, and illness perception. Methods: A total of 60 caregivers of autistic children were selected using convenient sampling method from a tertiary hospital in Wenzhou,Zhejiang Province. They were randomly divided as the intervention group and control group, with 30 cases in each. The control group was given routine health education, and the intervention group was given integrated behavioral model-based health education. A family assessment device (FAD), a tool to measure parenting selfefficacy
(TOPSE), and a brief illness perception questionnaire were used to assess family function, parenting selfefficacy,and illness perception. Results: After 6 months of intervention, each dimension of the family function in the intervention group was significantly higher than that in the control group (P<0.001), and the scores of both
affective and emotional subscales and pressure subscale of TOPSE in the intervention group were significantly higher than those in the control group (P<0.05). There was no statistical difference in illness perception between the two groups. Conclusion: Integrated behavioral model-based health education can improve the family
functional and parenting self-efficacy of caregivers of autistic children, but the effect on illness perception needs to be further explored.
2022 Vol. 52 (6): 465-471 [Abstract] ( 751 ) HTML (1 KB)  PDF (1459 KB)  ( 630 )
472 Construction of an individualized nomogram model for predicting the risk of pharyngocutaneous fistula in postoperative patients with laryngeal carcinoma
CHEN Qingsi, YANG Yeqin, WENG Chengjie, LIN Liqin, DAI Chaohui, CHEN Chengshui
DOI: 10.3969/j.issn.2095-9400.2022.06.007
Objective: To construct an individualized nomogram model for predicting the risk of pharyngocutaneous fistula in postoperative patients with laryngeal carcinoma. Methods: Altogether 252 patients who underwent total laryngectomy or partial laryngectomy in the First Affiliated Hospital of Wenzhou Medical University from January 2018 to July 2021 were divided into pharyngocutaneous fistula group (n=49) and non-pharyngocutaneous fistula group (n=203) according to whether pharyngocutaneous fistula occurred after operation. The data were compared between the two groups. Univariate analysis and Logistic regression analysis were made of the influencing factors of pharyngocutaneous fistula in patients with laryngeal carcinoma after operation; the risk prediction model was established and the nomogram was drawn; C-index, calibration curve and Hosmer-Lemeshow test were used to evaluate the prediction value of the model. Results: Tumor stage, preoperative radiotherapy history, nutritional risk and intraoperative flap repair were independent risk factors for pharyngocutaneous fistula in patients with postoperative laryngeal carcinoma. The calculated C-index was 0.842 (95%CI=0.776-0.908). The calibration curve confirmed that the predicted results of the model were in good
agreement with the actual results and Hosmer-Lemeshow goodness of fit test (χ2=7.564, P=0.477). Conclusion:Tumor stage, preoperative radiotherapy history, nutritional risk and intraoperative flap repair can increase the incidence of pharyngocutaneous fistula in patients with postoperative laryngeal carcinoma. The nomogram prediction model can effectively predict the incidence of pharyngocutaneous fistula after laryngeal carcinoma surgery.
2022 Vol. 52 (6): 472-477 [Abstract] ( 513 ) HTML (1 KB)  PDF (1480 KB)  ( 753 )
478 Metabolic characteristics of skimmin in rats by UPLC-Q-TOF/MS
XIAO Qiming, YU Xixi, YU Xinwei, YE Xiaoxia.
DOI: 10.3969/j.issn.2095-9400.2022.06.008
Objective: To identify and analyze the metabolic pathways of skimmin and its metabolites in bile,plasma, urine and feces of rats after administration of UPLC-Q-TOF/MS. Methods: Biological samples of rats were analyzed by UPLC-Q-TOF/MS before and after administration by intragastric or intravenous administration, and the mass spectrometry data of biological samples were obtained in the electrospray positive and negative ion mode; MassLynx V4.1 software was performed to process UPLC-Q-TOF/MS data; The metabolites of skimmin in bile, plasma, urine, and feces samples were identified. Results: In addition to the prototype, a total of 10 metabolites were identified in rat bile, plasma, urine, and feces samples. Among them, 8 metabolites were metabolized by the renal pathway. Conclusion: Hydrolysis, glucuronide conjugation, desaturation and hydroxylation were considered as the main metabolic pathways of skimmin in vivo. Considering the extensive metabolism in urine, attention should be paid to the clinical medication guidance of skimmin via kidney.
2022 Vol. 52 (6): 478-485 [Abstract] ( 404 ) HTML (1 KB)  PDF (2324 KB)  ( 532 )
486 Application of raw image superposition processing technique in angiography of brain CT perfusion
GUO Xianzhong, SONG Haolan, HE Shenping, SUN Han, WANG Zhenzhang, CHEN Weijian.
DOI: 10.3969/j.issn.2095-9400.2022.06.009
Objective: To evaluate the application of raw image superposition technique in CT perfusion (CTP) angiography. Methods: A total of 253 patients with chronic ischemic stroke who underwent CTP examination in the First Affiliated Hospital of Wenzhou Medical University from September 2019 to June 2021 were selected. Continuous dynamic enhanced sequence images were obtained by CTP examination from 22 groups, of which two groups were randomly selected as group A and group B, and two groups were superposed to obtain a new group of images as group C. CT values of internal carotid artery and adjacent temporal lobe,Std values of the three groups of images before and after superposition were measured respectively. The above operations were repeated for images of 253 patients, and the contrast to noise ratio (CNR) of the three groups of images of each patient was obtained by calculation. Results: The subjective scores of group C were higher than those of group A and group B before superposition, with significant difference (P<0.05). Moreover, the vascular display of group C was smoother and clearer, and the terminal vascular branch could also be clearly displayed, meeting the requirements of clinical diagnosis. Among them, the subjective scores of group C images were similar to those of cerebral arteriography images in some patients, with no statistical significance (P>0.05). The CNR of group C was higher than that of group A and group B, showing statistical difference (P<0.01). Conclusion: A new set of CTA images superimposed by Add/Sub can improve the clarity of intracranial vascular display and the
image quality of CTA, achieving the image effect similar to that of cerebral arteriography, which can shorten the selecting time to observe the intracranial vessels and the possible misdiagnosis rate caused by human factors.
2022 Vol. 52 (6): 486-489,493 [Abstract] ( 416 ) HTML (1 KB)  PDF (1608 KB)  ( 638 )
490 Group psychotherapy for opiate addicts with methadone maintenance treatment and its intervention effect
YANG Bingsheng, DAI Chunyan, CAO Chengjian.
DOI: 10.3969/j.issn.2095-9400.2022.06.010
Objective: To explore the intervention effect of group psychotherapy for opiate addicts with methadone maintenance treatment (MMT). Methods: A total of 203 patients from MMT clinic from March 2018 to March 2019 were randomly assigned to either local routine MMT (control group, n=100) or routine MMT plus group psychotherapy (intervention group, n=103). The scores of SCL-90, opiate-positive urine test results and treatment retention were compared between the two groups before and after intervention. Results: No significant difference was found in the total and each factor score of SCL-90 between the two groups before intervention (all P>0.05). After intervention, except for psychosis factor score (P=0.42), the total and other factor scores of SCL-90 in the intervention group were significantly lower than those in the control group (all P<0.05). Compared to the control group, the intervention group had lower proportion of opiate-negative urine tests and treatment retention after the intervention (all P<0.05). Conclusion: Group psychotherapy could effectively improve MMT patients’mental health while reducing the relapse rate and retention rate, thus further improving the efficacy of methadone maintenance treatment.
2022 Vol. 52 (6): 490-493 [Abstract] ( 409 ) HTML (1 KB)  PDF (1485 KB)  ( 591 )
494 Identification and analysis of a rare clinical case with AB and para-Bombay phenotype
CHEN Bile,HONG Junying, LIU Mengzao, XIE Zuoting.
DOI: 10.3969/j.issn.2095-9400.2022.06.011
Objective: To explore serological identification of blood type and genetic basis of a proband with para-Bombay AB blood type. Methods: ABH and Lewis antigens on the red blood cell (RBC), RBC irregular antibodies in plasma and the ABH blood group substances in saliva were detected by standard serologic techniques.Exons 6 and 7 of the ABO gene and exons of FUT1 and FUT2 genes were subjected to direct sequencing, and PCR amplification products were compared with the gene reference sequence to analyze the mutation sites by blast. Results: Serologic reaction results revealed that A and Leb antigens were present on the surface of RBCs from the proband, but B, H and Lea antigens were absent. A, B and H antigens were present in saliva, and the serum contained anti-H IgM antibody. The proband was proven to be a *A1.02/*B.01-type ABO gene carrier,DNA sequencing revealed that she has harbored c.551_552delAG and c.881_882delTT, and c.357C>T compound
heterozygous variants of the FUT1 gene, and c.357C>T homozygous point mutation was revealed in FUT2 gene. Conclusion: Heterozygous deletion of FUT1 genes were responsible for the Para-Bombay phenotype found in secreted AB type-proband. The para-Bombay blood type ABmh phenotype of the proband may be attributed tothe FUT1 gene compound heterozygous variants.
2022 Vol. 52 (6): 494-497 [Abstract] ( 347 ) HTML (1 KB)  PDF (1510 KB)  ( 646 )
505 The current status of overseas spread of Traditional Chinese Medicine culture and its enlightenment: book data analysis based on Amazon
MIAO Liyi, LYU Ying
DOI: 10.3969/j.issn.2095-9400.2022.06.014
As Traditional Chinese Medicine (TCM) is one of the representative elements of Chinese culture,promoting it overseas is an important way to advance China’s fine traditional culture worldwide and build national “soft power” as well. In view of its low popularity outside China, it is necessary to make a comprehensive investigation of TCM oversea acceptance in order to develop more targeted communication strategies. Based on the data analysis of the TCM book sales on the official website of Amazon, this study discusses the main concerns,disseminators, timelines and other issues of TCM international communication. Reflections and suggestions are
made from the perspective of communication for the TCM popularization overseas.
2022 Vol. 52 (6): 505-509 [Abstract] ( 390 ) HTML (1 KB)  PDF (1403 KB)  ( 1161 )
510 Systematic remodeling path of cadre work in colleges and universities——an analysis of the system construction research based on Wenzhou Medical University
SONG Yuya.
DOI: 10.3969/j.issn.2095-9400.2022.06.015
In the process of building China into an important talent center and innovation highland in the world in the new era, colleges and universities undertake a major mission of the times. The systematic remodeling of cadre work in colleges and universities and the construction of a loyal, clean and responsible team of highquality professional cadres are related to the development of colleges and universities and the realization of their mission. The author takes the governor and personnel system of Wenzhou Medical University as the research object, explores the mechanism of leading the high-quality development of cadre work based on the construction of the governor and personnel system, and deepens the understanding of the systematic remodeling of cadre work in colleges and universities, which can provide practical reference for the selection and use of manpower in relevant colleges and universities.
2022 Vol. 52 (6): 510-513 [Abstract] ( 606 ) HTML (1 KB)  PDF (1382 KB)  ( 739 )
514 The three-dimensional route for the integration of spirit of combating COVID-19 epidemic into curriculumbased character eductation in medical colleges and universities
SU Fengqi.
DOI: 10.3969/j.issn.2095-9400.2022.06.016
Curriculum-based character education focuses on cultivating the core values college and university students, which is the key to the success of educational reform and innovation in the new era. The great spirit of combating the COVID-19 epidemic has its unique value implication for the construction goal, content design and resource expansion of curriculum-based character education in medical institutions. In view of current difficulties in the curriculum-based character education in medical universities, this paper explores the threedimensional route to integrate spirit of combating the COVID-19 epidemic into curricular goal, teaching content and resources, so as to improve the practical effectiveness of curriculum-based character education.
2022 Vol. 52 (6): 514-516,封三 [Abstract] ( 363 ) HTML (1 KB)  PDF (1385 KB)  ( 557 )
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