温州医科大学学报
 
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2021 Vol. 51, No. 12
Published: 2021-12-25
 
 
947 The effect of FGF21 on learning and memory impairment induced by lactate accumulation in mice and its mechanism
JIANG Haowei, YI Qingqing, SHEN Danjie, GAO Hongchang, ZHAO Liangcai
DOI: 10.3969/j.issn.2095-9400.2021.12.001
Objective: To study whether fibroblast growth factor 21 (FGF21) can improve learning and memory impairment induced by lactate accumulation in mice and its potential mechanism. Methods: Male mice were randomly divided into three groups: control group, lactate group and lactate+FGF21 group. Y maze, Morris water and real-time quantitative PCR was used respectively to explore the learning and memory ability in different groups of mice. The specific metabolic changes of hippocampus were investigated by nuclear magnetic resonance based metabonomics methods. Results: The lactate group showed lower spontaneous exchange rate of Y maze, longer escape latency and shorter crossing platform, compared with the controls. The level of synaptic plasticity genes, including early growth response-1 (EGR-1), proto-oncogene (c-Fos), presynaptic (SYP) and postsynaptic proteins (PSD) (including PSD-93, PSD-95) mRNA level were significantly decreased in lactate group mice (P<0.05). FGF21 could restore the decline trend of learning and memory caused by lactate treatment(P<0.05). Metabonomic data showed taurine, inosine, creatine, inositol, glutathione, γ-aminobutyric acid,succinate, N-acetylaspartate, adenosine monophosphate and inosine acid decreased significantly in hippocampal extracts from the lactate mice, while their contents increased significantly after FGF21 treatment (P<0.05).Conclusion: FGF21 might improve the learning and memory dysfunction of mice by promoting lactate utilization,through restoring ATP-dependent energy metabolism pathways and NADH-related oxidative stress pathways.
2021 Vol. 51 (12): 947-953 [Abstract] ( 1371 ) HTML (1 KB)  PDF (1996 KB)  ( 714 )
954 Clinical characteristics of anti-MDA5 antibody positive dermatomyositis complicated with interstitial lung disease
CHEN Yongxian, WANG Liangxing, SHI Yubo, CHEN Jiajing, QIAN Qinqing, YUAN Jiawei, HUANG Xiaoying
DOI: 10.3969/j.issn.2095-9400.2021.12.002
Objective: To explore the clinical and imaging features of anti-melanoma differentiationassociated Gene 5 (MDA5) antibody-associated dermatomyositis (DM) combined with interstitial lung disease (ILD) for the benefit of the diagnosis and prognosis of the disease. Methods: We made a retrospective analysis of 81 DM patients with ILD who had been admitted to the Department of Rheumatology and the Department of Aspiration and Critical Care Medicine in the First Affiliated Hospital of Wenzhou Medical University from January 2017 to July 2021. According to test results of anti-MDA5 antibody, the patients were divided into two groups: 26 positive cases (positive group) and 55 negative cases (negative group). General data, imaging features, laboratory examination results and prognosis were analyzed, and the cases of death within half a year were counted. Results: Fever, joint pain, Gottron sign, V sign and skin rash were significantly different between the positive group and the negative group (P<0.05). The single factor analysis of imaging features showed that pleural effusion was more common in the positive group than that in the negative Group, with significant difference(P<0.05). The expression level of carcinoembryonic antigen (CEA), Cytokeratin 19 fragment (Cyfra21-1) and serum ferritin (SF) were higher in the positive group than in the positive group (P<0.05). The results of multivariate analysis showed that there was significant difference in high CEA (≥3.45 μg/L), high Cyfra21-1 (≥11.20 ng/mL) and high SF (≥561.65 μg/L) between the two groups (P<0.05). The area under the ROC curve (AUC) and the sensitivity of the combined detection of CEA and SF in the diagnosis of anti-MDA5 antibody positive DM with ILD were higher than those of the single indicator or combined detection of another two indicators or all three indicators, with the AUC being 0.756 (P<0.001). The sensitivity and specificity of the combined detection of CEA and SF for anti-MDA5 antibody positive DM with ILD were 80.8% and 70.9%, respectively. The death cases of positive group and negative group were 16(61.54%) and 10(18.18%), respectively. The prognosis difference was statistically significant (P<0.05). Conclusion: The clinical and imaging features of patients with anti-MDA5 antibody positive DM combined with ILD were various. The combined detection of CEA and SF is helpful to improve the diagnostic ability of anti-MDA5 antibody positive DM with ILD. The dynamic monitoring of CEA,Cyfra21-1 and SF may be helpful to evaluate the prognosis of anti-MDA5 antibody positive DM combined with ILD.
2021 Vol. 51 (12): 954-959 [Abstract] ( 659 ) HTML (1 KB)  PDF (1386 KB)  ( 952 )
960 Kaempferide inhibits the proliferation and migration of lung cancer cell A549 by down-regulating circ-RPL15
ZHENG Chengchao, MAO Jiabin, HE Daqiang
DOI: 10.3969/j.issn.2095-9400.2021.12.003
Objective: To explore whether kaempferide can affect the biological behavior of lung cancer cell A549 through circ-RPL15. Methods: Kaempferide of 25, 50, and 75 μg/mL was used to treat lung cancer cells A549. The dual luciferase report experiment was used to analyze the targeted regulation of miR-489-3p by circ-RPL15. circ-RPL15 was silenced or overexpressed in cell A549, and treated with 75 μg/mL kaempferol. Cell counting kit-8 (CCK-8) was applied to measure changes in the viability of lung cancer cells A549 in each group;plate cloning was performed to detect cell clone formation; flow cytometry was used to monitor cell apoptosis;Western blot was employed to detect the expression of Cleaved-caspase3 protein; Transwell method was used to detect cell migration, and real-time quantitative polymerase chain reaction (RT-qPCR) to determine the expression of circ-RPL15 and miR-489-3p. The dual luciferase report experiment analyzed the targeted regulation of miR-489-3p by circ-RPL15. Results: Compared with the control group, after different concentrations (25, 50 and 75 μg/mL) of kaempferide acted on lung cancer cell A549, cell viability, clone formation number, migration number and circ-RPL15 expression decreased, while apoptosis rate, Cleaved-caspase3 protein and miR-489-3p expression increased (P<0.05), and all were concentration-dependent. Silencing circ-RPL15 enhanced the expression of miR-489-3p, apoptosis rate and Cleaved-caspase3 protein expression of lung cancer cell A549, and weakened cell viability, the number of clone formation, and the number of migration (P<0.05). circ-RPL15 targets miR-489-3p. Overexpression of circ-RPL15 reversed the proliferation, apoptosis and migration of lung cancer cells that were affected by kaempferide (P<0.05). Conclusion: Kaempferide inhibits the proliferation and migration of lung cancer cells and induces their apoptosis by down-regulating circ-RPL15 and targeting miR-489-3p.
2021 Vol. 51 (12): 960-967 [Abstract] ( 566 ) HTML (1 KB)  PDF (2681 KB)  ( 605 )
968 Clinical application of 18F-FDG-PET/CT combined with magnetic resonance imaging in predicting preoperative T and N staging of colorectal cancer
ZU Liang, WANG Zufei, CHEN Weiyue,WU Xulu, XIAO Yangrui, JI Jiansong
DOI: 10.3969/j.issn.2095-9400.2021.12.004
Objective: To explore the clinical application value of 18F-FDG-PET/CT combined with magnetic resonance imaging (MRI) in predicting preoperative T and N staging of colorectal cancer. Methods:Patients who had surgical resection for colorectal cancer in our hospital were retrospectively enrolled from January 2015 to October 2019. All patients underwent 18F-FDG-PET/CT and MRI scans within two weeks before the operation, with postoperative pathological results taken as the gold standard. The consistency test was performed between test results and pathology, and chi-square test was used to compare the accuracy, sensitivity,and specificity of combined and single examination. Results: A total of 92 patients were enrolled in the study,including 55 males and 37 females, with the average age of (64.7±10.6)years. The study showed that the T staging results of the combined examination had a strong consistency with the pathology (Kappa value was 0.888). The accuracy (93.48%) was higher than that of PET/CT alone (69.57%), which showed statistically significant difference (P<0.001), but had no obvious advantage compared with MRI alone (P=0.090). Meanwhile,the N staging results of the combined examination had a strong consistency with the pathology (Kappa=0.804),and the accuracy (88.04%) was higher than that of MRI (67.39%) and PET/CT (75.00%), with significant difference (P=0.001 and 0.023). Furthermore, the combined examination had better, the accuracy (93.48%)and specificity (93.75%) in the diagnosis of lymph node metastasis than MRI (P=0.001 and 0.002) and PET/CT alone (P=0.037 and 0.037). Conclusion: Preoperative 18F-FDG-PET/CT combined with MRI has high diagnostic efficiency for preoperative T and N staging of colorectal cancer, especially in the evaluation of N staging and lymph node metastasis, which is of strong clinical application value.
2021 Vol. 51 (12): 968-973 [Abstract] ( 578 ) HTML (1 KB)  PDF (1602 KB)  ( 661 )
974 Ferulic acid inhibits the invasion and migration of cervical cancer HeLa cells by regulating mitochondrial apoptosis in vitro
XIA Lu, HUANG Min, MEI Jie, YAN Linzhi
DOI: 10.3969/j.issn.2095-9400.2021.12.005
Objective: To study the effect of Ferulic Acid (FA) on cervical cancer (HeLa) cell lines and its possible mechanism. Methods: HeLa cells were cultured in vitro and divided into the control group and FA treatment group (1, 2 and 4 mmol/L). CCK-8 and scratch test detected cell proliferation and migration ability.Transwell test evaluated cell invasion ability. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of apoptosis-related genes Bcl-2, Bax and Cleaved-caspase-3 in HeLa cells after treatment with different concentrations of FA, and immunofluorescence was used to detect the expression and status of mitochondria in HeLa cells. Results: RT-qPCR results showed that the expression of Bcl-2 gene in HeLa cells gradually decreased, while the expression of Bax and Cleaved-caspase-3 genes gradually increased in a dose-dependent manner, with statistical difference (P<0.05), compared with the control group.Immunofluorescence showed that the content of mitochondria in HeLa cells gradually decreased and the function gradually weakened with statistical difference (P<0.05), compared with the control group. The results of Transwell and scratch experiments showed that different concentrations of FA reduced the number of invaded cells and migrated cells of HeLa cells in a dose-dependent manner with significant differences (P<0.05),compared with the control group. Conclusion: FA inhibits the proliferation, invasion and migration of HeLa cells through the Bcl-2 family protein pathway, and induces mitochondrial-mediated apoptosis.
2021 Vol. 51 (12): 974-979 [Abstract] ( 514 ) HTML (1 KB)  PDF (2105 KB)  ( 786 )
980 Differences in imaging evaluation of fetal lateral ventricle and (or) posterior cerebral fossa cistern enlargement with pathogenic copy number variation
ZHAO Shunbiao, HONG Weifeng, WANG Rongyue,XIA Jianli, XU Min, HUANG Deyi, ZHAO Yaping, XU Chongyong
DOI: 10.3969/j.issn.2095-9400.2021.12.006
Objective: To investigate the difference between the imaging evaluation of fetal lateral ventriculomegaly (VM) and/or posterior cerebral fossa cistern enlargement (PCFCE) and pathogenic copy number variants (CNVs). Methods: From January 2015 to October 2020, the fetuses diagnosed with VM and/or PCFCE were collected from the Second Affiliated Hospital of Wenzhou Medical University and Yuhuan People’s Hospital by prenatal ultrasound. A total of 1 114 cases, of which 468 fetuses underwent MRI and karyotype analysis (16-24 week, amniotic fluid detection in 317 cases; 24-38 week, cord blood test in 151 cases), and 440 fetuses with normal karyotype underwent whole-genome high-resolution chromosome microarray analysis (CMA) detection.Difference between fetal VM and/or PCFCE degree with malformations in all systems and CNVs was analyzed,pregnancy outcome was evaluated. Results: Among 468 fetuses, the incidence rate in abnormal chromosomal karyotypes was 5.98% (28/468), of which 11 were abnormal in number, 10 in structure, 7 in number+structure,and all 28 cases induced labor; Among 440 fetuses with normal karyotype, the incidence of pathogenic CNVs was 8.41% (37/440). Among 440 fetuses with normal karyotype, the incidence of pathogenic CNVs in severe VM and/or PCFCE group was higher than that in mild to moderate group (13.71% vs. 6.33%, P=0.012).The incidence of pathogenic CNVs among simple VM, simple PCFC and VM+PCFCE was VM+PCFCE >simple PCFCE > simple VM (12.14% vs. 11.69% vs. 4.93%, P=0.029). Among 37 cases of CNVs, 5 cases were complicated with central nervous system malformation, 4 cases were complicated with genitourinary system, 5 cases were complicated with cardiac macrovascular malformation, 2 cases were complicated with digestive system malformation. The incidence of fetal malformation in severe VM and/or PCFCE group was higher than that in mild to moderate group (7.26% vs. 2.22%, P=0.020). Among the 37 fetuses with pathogenic CNVs, 23 cases were induced labor and 14 cases were delivered. Conclusion: The incidence of pathogenic CNVs and fetal malformations was higher in severe VM and/or PCFCE group, and the pathogenic CNVs in VM+PCFCE group was higher than that in simple VM or PCFCE group. Accurate imaging evaluation is helpful for intrauterine outcome, clinical evaluation and genetic counseling of VM and/or PCFCE fetuses.
2021 Vol. 51 (12): 980-984 [Abstract] ( 668 ) HTML (1 KB)  PDF (2426 KB)  ( 661 )
985 Changes of vascular endothelial growth factor-A in patients with Crohn’s disease after treatment with infliximab and its clinical significance
CHENG Haiyan, SHEN Zhe
DOI: 10.3969/j.issn.2095-9400.2021.12.007
Objective: To investigate the changes and clinical significance of vascular endothelial growth factor-A (VEGF-A) in patients with Crohn’s disease after treatment with infliximab. Methods: From January 2016 to September 2020, 30 patients with severe CD and 30 healthy volunteers were selected. Patients with severe CD were treated with infliximab for 6 months. The serum and intestinal mucosa were collected from CD patients before and after treatment and from healthy volunteers as well. Enzyme linked immunosorbent assay (ELISA) and immunohistochemical staining were used to detect the expression of VEGF-A in serum and intestinal mucosa.C-reactive protein (CRP) was detected by scattered light turbidimetry and erythrocyte sedimentation rate (ESR) was detected by Windmanstatten method. Results: ELISA showed that VEGF-A expression in the serum of CD patients was significantly lower than that before treatment, but higher than healthy volunteers (P<0.05).After treatment, CD patients had significantly decreased CRP and ESR serum levels, compared with the healthy volunteers. The serum CRP level in the post-treatment group increased significantly (P<0.05), but there was no significant difference in ESR level (P=0.521). There were positive correlations between CRP, ESR and VEGF-A in CD patients before treatment (r=0.727, 0.450, P<0.05). Immunohistochemical staining showed that VEGF-A expression in the diseased intestinal tissue of CD patients was significantly lower than that before treatment, but higher than the healthy controls (P<0.05). The expression level of VEGF-A in serum was positively correlated with the expression intensity of VEGF-A in intestinal mucosa tissue before and after treatment (r=0.714, 0.926,P<0.05). Conclusion: The VEGF-A level in serum and intestinal mucosa is positively correlated with the CD disease activity, which has the potential to be used as an indicator to evaluate the treatment efficacy of CD.
2021 Vol. 51 (12): 985-989,994 [Abstract] ( 515 ) HTML (1 KB)  PDF (1580 KB)  ( 616 )
990 The protective effect of docosahexaenoic acid on traumatic brain injury and its underlying mechanism
JIN Can, ZHANG Renkan, ZHANG Kairui, CHEN Daqing
DOI: 10.3969/j.issn.2095-9400.2021.12.008
Objective: To investigate the protective effect of docosahexaenoic acid (DHA) on traumatic brain injury (TBI) and its potential mechanism. Methods: Controlled cortical impact was used to establish an animal model of TBI. Forty-five C57BL/6 mice, in accordance with the random number table, were divided as the Sham group, the TBI group, the TBI+DHA group, with fifteen mice in each. After operation, the TBI+DHA group was intraperitoneally injected with DHA at the dose of 10 mg/kg/day within 30 minutes for three days. The Sham group and the TBI group were injected with the same volume of 0.9% sodium chloride solution. Garcia scores was performed on 21 days after TBI to assess the recovery of motor sensory function of each group.Hematoxylin eosin (HE) staining was used to observe the lesion of cerebral cortex of mice. Western blot and realtime fluorescence quantitative polymerase chain reaction (RT-qPCR) were used to detect the inflammation-related proteins such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) expression and corresponding nuclear-related factors. Results: Compared with the Sham group, the Garcia score in the TBI group significantly decreased (P<0.05), while that of DHA group increased significantly (P<0.05). Compared with the Sham group, the TBI group had obvious cerebral cortex tissue loss. However, the DHA drug treatment could reduce the cerebral cortex loss and promote its repair. Western blot and RT-qPCR showed that the protein expression of TNF-α, IL-6 and its mRNA increased significantly, while the expression of IL-10 gene and its mRNA decreased significantly in the TBI group (P<0.01), compared with the Sham group; the expression of TNF-α and IL-6 and its mRNA significantly decreased, while the expression of IL-10 significantly increased after DHA treatment (P<0.01) compared with the TBI group. Conclusion: DHA can produce the protective effect on traumatic brain injury, probably through regulating TNF-α signal pathway.
2021 Vol. 51 (12): 990-994 [Abstract] ( 572 ) HTML (1 KB)  PDF (1584 KB)  ( 646 )
995 Linc00346 promotes proliferation of bladder cancer cells via PI3K/Akt signaling pathway through inhibiting PTEN
YE Tingyu, HUANG Hang, PAN Yue, CAI Bing
DOI: 10.3969/j.issn.2095-9400.2021.12.009
Objective: To study the molecular mechanism of linc00346 in promoting proliferation of bladder cancer cells. Methods: Real-time fluorescence quantitative PCR (RT-PCR) was used to detect the expression of linc00346 and PTEN in urothelial carcinoma tissues of bladder. The expression level of linc00346 and PTEN in bladder cancer cell lines T24 was detected by RT-PCR after knockdown linc00346. The expression of PTEN protein and Akt, p-Akt protein in PI3K/Akt signaling pathway was measured by Western blot; The cell proliferation ability was determined by CCK-8 method. Results: The expression of linc00346 was up-regulated, while the PTEN expression level was down-regulated in urothelial carcinoma tissues (P<0.05). After knockdown linc00346 in T24 cells, the expression level of linc00346 decreased, the level of PTEN mRNA increased (P<0.05), the expression of PTEN protein increased, and the expression of p-Akt reduced (P<0.05). When PTEN inhibitor SF1670 was given at the same time, the expression of PTEN protein was down-regulated, and that of p-Akt was up-regulated (P<0.05). The proliferation ability of T24 cells was inhibited obviously after silencing linc00346.Furthermore, SF1670 made the cell proliferation ability increased (P<0.05). Conclusion: Linc00346 can increase the proliferation ability of bladder urothelial carcinoma cells by inhibiting PTEN, which activates PI3K/Akt signaling pathway.
2021 Vol. 51 (12): 995-999 [Abstract] ( 616 ) HTML (1 KB)  PDF (1935 KB)  ( 895 )
1000  Evidence-based nursing practice of management of safe medication for nurses in operating room
XU Duo, HAN Yongqi, XU Xiaoqun
DOI: 10.3969/j.issn.2095-9400.2021.12.010
Guide:: 
 Objective: To explore the best Evidence-based nursing practice of management of safe medication for nurses in operating room and evaluate its clinical effects. Methods: To obtain evidence through evidencebased nursing, and was implemented in clinical practice after localization. We formulated the review standard
of intraoperative medication use for operating room nurses, and standardized the intraoperative medication use behavior of operating room nurses; Various training methods were adopted to train and assess the best evidence for the operating room nurses, the awareness rate of the operating room nurses on medical-related knowledge and the actual verification rate of safe medication use in the operating room before and after training were also compared. Results: After adapting the best evidence, the awareness rate of operating room nurses on medical verification process, medical partition placement, medical labeling application and other aspects was improved, the differences were statistically significant (P<0.05). And the implementation rate of the six verification items of safety medication use practice of operating room nurses also increased with statistical significance (P<0.05).
Conclusion: Medication error for nurses in operating room can be solved by using scientific nursing methods through implementation of best evidence, in order to nursing quality in operating room.
2021 Vol. 51 (12): 1000-1005 [Abstract] ( 648 ) HTML (1 KB)  PDF (1361 KB)  ( 1008 )
1006  Effect of cognitive behavioral intervention therapy on psychological emotion and pain in children with traumatic fracture complicated with PTSD
LIN Hainiao, JIA Man, LI Qun, ZHANG Xiuxia, ZHANG Chunmei
DOI: 10.3969/j.issn.2095-9400.2021.12.011
Guide:: 
 Objective: To discuss the effect of cognitive behavioral intervention therapy on psychological
emotion and pain in children with traumatic fracture combined with PTSD. Methods: From March 2019 to September 2019, 240 children with traumatic fracture and PTSD were treated in emergency and pediatric orthopaedic ward of our hospital. They were divided into two groups according to nursing intervention methods,120 cases in each group. The control group received general nursing measures, while cognitive behavioral intervention therapy was added to the interference group on the basis of the control group. The scores of posttraumatic stress disorder self-rating scale (PTSD-SS), somatization symptom checklist 90 (SSS), self-rating anxiety scale (SAS), visual analogue scale (VAS) and Peds QL4.0 were compared between the two groups. The quality of life of the two groups was compared, and the parents’ satisfaction with nursing was compared between the two groups. The influence of psychological emotion and pain in children was observed. Results: After intervention, the scores of PTSD-SS, SSS, VAS, SAS, Peds QL4.0 and satisfaction questionnaire questionnairein the interference group were lower than those before treatment (P<0.05), and were significantly lower than those in the control group (P<0.05). The nursing satisfaction of family members in the interference group was significantly higher than that in the control group (P<0.05). Conclusion: Cognitive behavioral interventiontherapy can effectively reduce PTSD incidence in children with traumatic fracture, relieve psychological anxiety and somatic symptoms, alleviate their pain feelings, promote their early rehabilitation and improve their quality
of life, thus their amily saltisfaction is high.
2021 Vol. 51 (12): 1006-1009，1013 [Abstract] ( 758 ) HTML (1 KB)  PDF (1434 KB)  ( 837 )
1010  
DOI: 10.3969/j.issn.2095-9400.2021.12.012
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2021 Vol. 51 (12): 1010-1013 [Abstract] ( 504 ) HTML (1 KB)  PDF (1489 KB)  ( 641 )
1014  
DOI: 10.3969/j.issn.2095-9400.2021.12.013
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2021 Vol. 51 (12): 1014-1016 [Abstract] ( 463 ) HTML (1 KB)  PDF (1509 KB)  ( 717 )
1017  
DOI: 10.3969/j.issn.2095-9400.2021.12.014
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2021 Vol. 51 (12): 1017-1019 [Abstract] ( 453 ) HTML (1 KB)  PDF (1374 KB)  ( 791 )
1020  
DOI: 10.3969/j.issn.2095-9400.2021.12.015
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2021 Vol. 51 (12): 1020-1024 [Abstract] ( 670 ) HTML (1 KB)  PDF (1388 KB)  ( 835 )
1025  Study on the knowledge, attitude and determinants of non-related peripheral blood stem cells in registered volunteers’ family members
DING Nani, JIN Xinqian, Chen Zewei, ZHANG Sha, ZHANG Guohu.
DOI: 10.3969/j.issn.2095-9400.2021.12.016
Guide:: 
 Objective: To investigate the knowledge, attitude and determinants of non-related peripheral
blood stem cells (PBSC) donation in registered PBSC volunteers’ family members. Methods: Modified standardstructure questionnaire was used to collect the information on the knowledge, attitude and the associated influentia factors of PBSC donation among family members of 60 PBSC donors and 240 registered volunteers. Results: As compared to those family members of registered BPSC volunteers, the score of the knowledge, attitude and some critical determinants of PBSC donors’ relatives were both significantly higher than those of their counterparts. In addition, the two evidently influential factors, such as knowing Chinese marrow donor program and the experience of unpaid blood donation, were detected as the major determinants on their practices. Conclusion: Our findings strongly suggest that appropriate strategy and measures on efficiently reducing the rate of regret donation should be paid on largely raising the knowledge, improving the attitude, and providing more information on the donationrelated
policy among those registered volunteers’ family members
2021 Vol. 51 (12): 1025-1029 [Abstract] ( 507 ) HTML (1 KB)  PDF (1348 KB)  ( 675 )
1030  
DOI: 10.3969/j.issn.2095-9400.2021.12.017
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2021 Vol. 51 (12): 1030-1032,封三 [Abstract] ( 1180 ) HTML (1 KB)  PDF (1266 KB)  ( 1329 )
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