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2021 Vol. 51, No. 4 Published: 2021-04-25   259 Construction of SARM1 conditional knockout mice in nervous system and its application XIANG Ludan, SUN Huankun, WU Qian, WANG Wei, HUANG Zhihui, YU Xin DOI: 10.3969/j.issn.2095-9400.2021.04.001 Objective: To construct neuronal-specific conditional SARM1 knockout mice and to provide a research tool for exploring the function of SARM1 in the nervous system. Methods: SARM1flox/flox transgenic mice were generated by using embryonic stem (ES) cell targeting technology. Next SARM1flox/flox transgenic mice were crossed with Nestin-Cre transgenic mouse to generate neuronal-specific conditional SARM1 knockout mice. After that the conditional SARM1 KO mice were genotyped by using polymerase chain reaction (PCR). Western blot was performed to confirm the loss of SARM1 protein in the brain tissues of these mice. Behavior tests such as open field and elevated plus maze test were performed to assess any emotional behavior and general anxiety level in these mice. Results: SARM1 protein was highly expressed in the central nervous system, which was mainly expressed in neurons. Genotyping results revealed that neuronal-specific conditional SARM1 knockout mice were successfully generated. Further more, compared with wild type mice, SARM1 protein level was significantly decreased in brain tissues of SARM1Nestin-CKO mice (P<0.05). Conditional knockout of SARM1 gene in central nervous system did not affect the growth and development of mice, or the general morphology of brain tissue and the number of neuronal cells. Meanwhile, these mice had no obvious anxiety disorder phenotype. Conclusion: We have successfully generated a mouse line that specifically knocks out the SARM1 gene in neuronal cells. The application of this mutant mouse line may provide a useful tool for exploring the role of SARM1 gene in neuropsychiatric diseases and neurodegenerative diseases. 2021 Vol. 51 (4): 259-265 [Abstract] ( 1200 ) HTML (1 KB)  PDF (2058 KB)  ( 790 ) 266 The effect of Quercetin on human nasopharyngeal cancer cell growth and AKT/mTOR expression ZHANG Jiafeng, ZHAO Hui, HE Ping, HAO Yalin, DONG Qiongna, JIN Xiaojie. DOI: 10.3969/j.issn.2095-9400.2021.04.002 Objective: To investigate the effect of quercetin on cell growth and AKT/mTOR pathway expression in human nasopharyngeal carcinoma 5-8F cells. Methods: 5-8F cells were treated with different concentrations of quercetin (20, 40 and 60 μmol/L) for 24 h, 48 h and 72 h before MTT assay was performed to detect the cell growth. Quantitative PCR and Western blot were used to detect the expression levels of AKT and mTOR. A nasopharyngeal carcinoma mouse model was established through subcutaneously inoculated with 5-8F cells in nude mice. The mouse model was randomly divided into the control group and  quercetin-treated group. Tumor volume was measured every 7 days. Western blot and immunohistochemistry were used for detection of AKT and mTOR protein levels in tissues. Results: 5-8F cells growth was significantly inhibited in the quercetin-treated group (P<0.05), compared with the control group. Quercetin reduced the expression levels of AKT and mTOR in 5-8F cells (P<0.05). In in vivo experiments, the tumor volume in nude mice in the quercetin-treated group was significantly inhibited (P<0.05), and the expression of AKT and mTOR in tumor tissues was also significantly decreased (P<0.05). Conclusion: Quercetin inhibits cell growth and AKT/mTOR pathway expression in nasopharyngeal carcinoma 5-8F cells 2021 Vol. 51 (4): 266-270 [Abstract] ( 652 ) HTML (1 KB)  PDF (1754 KB)  ( 784 ) 271 Expression profile of methylation regulated lncRNA and its significance in breast cancer HU Qian, LAN Xiaoshan, LIN Yingxin, CHEN Xiuyun, DING Linxiaoxiao, PANG Danmei DOI: 10.3969/j.issn.2095-9400.2021.04.003 Objective: To study the differential expression of lncRNA between demethylated breast cancer cells and their parental lines, and to screen lncRNA from differential expression profile, so as to explore the role of lncRNA regulated by DNA promoter methylation in the occurrence and development of breast cancer. Methods: After demethylation of breast cancer BT474 cell line, the expression profiles of lncRNA in demethylation group and parental group were detected by high-throughput lncRNA microarray technology, and the expression of lncRNA in breast cancer cell line BT474, MB231, BT549 and cancer tissue were detected by qRT-PCR technology. Meanwhile, the gene methylation sites were searched by bisulfite sequencing PCR. Results: LncRNA expression profiles of demethylated and parental cells have significantly changed. The results of quantitative RT-PCR and microarray were basically consistent. Quantitative RT-PCR showed that the expression of lncRNA uc003lxs and uc002btf in breast cancer tissues and breast cancer cell lines were higher than that in normal breast epithelial cell lines (P<0.01), and the expression of lncRNA uc003lxs and uc002btf in breast cancer tissues was higher than that in adjacent tissues (P<0.01). Compared with normal breast epithelial cell lines, the DNA methylation levels of lncRNA uc003lxs and uc002btf in breast cancer tissues and breast cancer cell lines were significantly decreased by BSP method (P<0.01); compared with adjacent tissues, the DNA methylation levels of lncRNA uc003lxs and uc002btf in breast cancer tissues were significantly decreased (P<0.01). Conclusion: The expression of lncRNA in breast cancer cell lines after demethylation treatment has been found to be significantly changed, and lncRNA uc003lxs and uc002btf have been screened out, which lays a preliminary research foundation for the study of methylation regulated lncRNA in the regulation of the occurrence and development of breast cancer. 2021 Vol. 51 (4): 271-275,281 [Abstract] ( 700 ) HTML (1 KB)  PDF (1661 KB)  ( 647 ) 276 The value of automatic cardiac motion quantification technique in evaluating left ventricular systolic function of frequent premature ventricular contractions patients with ejection fraction preserved XIAO Lili, SONG Jing, CHEN Xiaole, LIN Lingling, DONG Yanyan, WU Daozhu DOI: 10.3969/j.issn.2095-9400.2021.04.004 Objective: To explore the value of automated cardiac motion quantification (aCMQ) in evaluating left ventricular systolic function of frequent premature ventricular contractions (frequent PVCs) patients with ejection fraction preserved. Methods: From January 2018 to January 2019, 34 cases of frequent PVCs with ejection fraction preserved (LVEF>50%) were enrolled. At the same time, 27 healthy persons matched by sex were selected as the control group. The left atrial size (LAD), left ventricular end diastolic diameter (LVIDd), left ventricular end systolic diameter (LVIDs), left ventricular ejection fraction (LVEF) and diastolic function parameters (E/A and E/E’a, E’a referring to the average mitral annulus velocity of lateral and septal sides) were measured by conventional echocardiography. The main outcome assessments obtained by aCMQ technique were longitudinal strains of apex two-chamber view (AP2LS), apex three-chamber view (AP3LS), apex four-chamber view (AP4LS) and global longitudinal strain (LVGLS). Circumferential strains in three different levels of left ventricular in short axis, including basal level (SAXBCS), mid level (SAXMCS), apical level (SAXACS) and global circumferential strain (LVGCS) were also obtained. Results: There was no significant difference in age and body surface area between frequent PVCs group and control group (P>0.05). There was significant difference in LVIDd and LVIDs between the two groups (all P<0.05), but no significant difference in LAD, LVEF, E/A and E/E’a (all P>0.05). The absolute values of AP2LS, LVGLS and SAXMCS in frequent PVCs group were lower than those in the control group, showing significant differences (all P<0.05). ROC curve analysis showed AP2LS and LVGLS had a better predictive value for LV systolic dysfunction in frequent PVCs patients. Conclusion: ACMQ can detect the early subtle changes of cardiac function in frequent PVCs, and it can be used as a new method to detect the subtle LV dysfunction. 2021 Vol. 51 (4): 276-281 [Abstract] ( 596 ) HTML (1 KB)  PDF (1633 KB)  ( 709 ) 282 Effect of low-dose dobutamine on intraoperative cerebral oxygen saturation and postoperative delirium in elderly patients with one-lung ventilation FANG Ping, LOU Yingying, SUN Gangqiang, WANG Jingyu, XU Guodong DOI: 10.3969/j.issn.2095-9400.2021.04.005 Objective: To explore the effect of low-dose dobutamine on intraoperative rScO2 and POD in elderly patients undergoing one-lung ventilation. Methods: Sixty-four elderly patients undergoing OLV under general anesthesia were randomly divided as observation group (group O) and control group (Group C), with 32 cases in each. Dobutamine hydrochloride or normal saline was injected intravenously after OLV, with the speed of 1.5 μg/(kg·min) until the end of OLV. In addition to routine monitoring, the rScO2 was recorded before anesthesia (T1), 5 minutes before OLV (T2), 15 minutes after OLV (T3), 30 minutes after OLV (T4), 45 minutes after OLV (T5), and 15 minutes after OLV (T6). The agitation in recovery period, VAS and POD incidence of the two groups were observed on the 1st, 3rd and 7th day after operation (or before discharge). The change rate of rScO2 in both groups was calculated. The correlation between the change rate of rScO2 and CAM score, Riker sedation-Agitati score, POD were analyzed. The independent risk factors of POD were analyzed by Logistic regression. Results: During the period of OLV, the rScO2 of group C decreased gradually with time (P<0.05), while group O had significant rScO2 decrease at T5 (P<0.05). The HR and rScO2 at T5 in group O were higher than those in group C (P<0.05). The change rate of rScO2, agitation during recovery period, CAM score and incidence of POD in group O were lower than those in group C (P<0.05). The change rate of rScO2 was positively correlated with Riker sedation-Agitati score during recovery period and CAM score on 1st day after operation. Logistic regression analysis showed that the increase of rScO2 change rate, diabetes history and old age were independent risk factors for POD. Conclusion: Low-dose dobutamine can reduce the change rate of cerebral oxygen saturation and postoperative delirium in elderly patients with one-lung ventilation. 2021 Vol. 51 (4): 282-286 [Abstract] ( 640 ) HTML (1 KB)  PDF (1427 KB)  ( 596 ) 287 Clinical analysis of 54 cases of neonatal group B streptococcus septicemia LIU Jiahui, LIU Caixia, ZHANG Ying, YANG Jinhong, JIN Susu, LI Fangqu DOI: 10.3969/j.issn.2095-9400.2021.04.006 Objective: To explore the clinical characteristics, perinatal risk factors, drug resistance, treatment and regression of neonatal group B streptococcus (GBS) invasive infection. Methods: Clinical data of 54 neonates, who were admitted to the department of neonatology from January 2014 to June 2020, diagnosed with GBS invasive infection by sterile lacunar culture of blood and cerebrospinal fluid were collected and their perinatal risk factors, clinical manifestations, in vitro drug sensitivity test results, treatment and return were retrospectively analyzed. Results: Of 54 infants with GBS invasive infection, 28 had early-onset infection and 26 had late-onset infection. GBS was detected in both blood and cerebrospinal fluid samples in 13 cases, while GBS was detected only in blood in 36 cases and only in cerebrospinal fluid in 4 cases. Early-onset infections had cyanosis, groaning, shortness of breath and fever as the main symptoms, and were prone to pneumonia, and a small number of them have purulent meningitis. Late-onset infections had fever and poor feeding as the first symptoms, and were easily associated with purulent meningitis. The proportion of early-onset intrauterine infection was higher than late-onset, with significant difference (P<0.05). Three newborns who died had one or more perinatal risk factors. GBS drug susceptibility results showed 100% sensitivity to penicillin, ampicillin, vancomycin, and linezolid. After treatment, 47 cases were cured and discharged, 4 cases were discharged unhealed (due to family give-up), and 3 cases died (all early onset, with fatality rate of 5.5%). The average length of hospital stay was (25.5±15.6)d. Conclusion: For neonates with invasive GBS infection, laboratory pathogenic examinations should be performed according to the clinical characteristics as soon as possible to confirm the diagnosis. Penicillin should be the first choice, and combined with other antibiotics to fight against the infection according to the condition. Perinatal risk factors may be important for neonatal GBS infection. GBS screening and intrapartum antibiotic prevention (IAP) measures for pregnant women can effectively reduce the incidence of neonatal GBS infection. 2021 Vol. 51 (4): 287-291 [Abstract] ( 642 ) HTML (1 KB)  PDF (1405 KB)  ( 652 ) 292 Changes in characteristics and pregnancy outcomes of elderly puerpera in Wenzhou from 2015 to 2019 YE Aoshuang, LIN Ying, BAO Jiajia, YANG Xinjun DOI: 10.3969/j.issn.2095-9400.2021.04.007 Objective: To analyze the pregnancy characteristics and adverse pregnancy outcomes in elderly pregnant women, and to provide scientific basis for improving the working method and management mode of maternal and child health care to ensure the safety of mothers and infants. Methods:Retrospective analysis was made of the data of pregnant women aged ≥20 years who gave birth in Wenzhou midwifery institutions from 2015 to 2019, based on the related information collected from the Information Management Platform of Wenzhou Maternal and Child Health Center, and they were divided as the senior-age group (aged ≥35 years) and the right-age group (aged 20-34 years). Cochran-Mantel-Haenszel χ2 test and crosstabs χ2 test was used to analyze the linear trend in the incidence of adverse pregnancy outcomes and differences in demographics and pregnancy complications/comorbidity. Results: A total of 536 456 women gave birth in Wenzhou between 2015 and 2019, of whom 56 637 (10.56%) were elderly and 8 787 (15.51%) were aged ≥40 years old, with a maximum age of 55 years. Between 2015 and 2019, the proportion of elderly pregnant women in Wenzhou showed an upward trend (χ2trend=1 225.00, P<0.001), and the proportion of elderly pregnant women in parity ≥2 showed an upward trend (χ2trend=21.64, P<0.001). The proportion of non-first births, non-births, caesarean sections, education of junior high school and below were all higher in the senior-age group than those in the right-age group (P<0.001 in all comparisons). The incidence of gestational complications (GDM, pre-eclampsia) and complications (postpartum haemorrhage, premature fetal membrane breakage) in both groups showed an annually increasing trend (P<0.01), but the incidence of preterm birth, macrosomia and low birth weight in the senior-age pregnant women did not show an annual increasing or decreasing trend (P>0.05). The incidence of pregnancy complications and adverse pregnancy outcomes in the senior-age pregnant women was higher than the right-age group in 2015-2019 (P<0.001). Conclusion: The incidence of gestational diabetes and premature fetal membrane break-up among elderly pregnant women increased significantly from 2015 to 2019, and the incidence of adverse pregnancy outcomes in elderly pregnant women was higher than the right-age pregnant women. Therefore, obstetric doctors and health workers in the Maternal and Child Health Center need to pay more attention to the elderly pregnant women. 2021 Vol. 51 (4): 292-297 [Abstract] ( 569 ) HTML (1 KB)  PDF (1430 KB)  ( 728 ) 298 Effect of ultra-early intervention on nerve and motor development in infants at high risk of cerebral palsy WANG Chen, ZHANG Chao, PAN Shaohua DOI: 10.3969/j.issn.2095-9400.2021.04.008 Objective: To explore the effect of ultra-early intervention on the improvement of nerve and motor development in infants at high risk of cerebral palsy. Methods: From January 2017 to January 2018, 53 newborns, who were born in Xiasha District-Zhejiang Hospital of TCM and diagnosed as infants at high risk of cerebral palsy, were enrolled in the observation group, 46 infants at high risk of cerebral palsy (3-5 months old), who came to Zhejiang Rehabilitation Hospital in the same period, were enrolled in the control group, and 50 healthy newborns, who were born in Xiasha District-Zhejiang Hospital of TCM in the same period, were enrolled in the healthy group. The children in the observation group were intervened after diagnosis (within 7 days after birth), and the Neurodevelopmental therapy (NDT) was mainly used. The intervention measures and procedures used in the control group were the same as those of the observation group. The scores of development quotient (DQ), Gesell development scale (adaptability/the gross motor/fine motor/language/personal social contact) were observed among the three groups at each month, and were compared at 24 months of age between the observation group and the control group in the proportion of patients who reached normalization, progressed to cerebral palsy and lagged in overall development. Results: The scores of DQ in each functional area, total DQ, Gesell development scale (adaptability/the gross motor/fine motor/language/personal social contact) of the observation group were significantly lower than those of the healthy group (P<0.05) at baseline and 3 months old, and the scores of DQ in each functional area, total DQ, Gesell development scale (adaptability/the gross motor/fine motor/language/personal social contact) of the three groups were statistically significant (P<0.05) at 6, 12, 18 and 24 months old. The scores in the observation group were significantly higher than those in the control group (P<0.05). The rate of normalization in observation group was higher than in control group, and the incidence of cerebral palsy and general underdevelopment was lower than that of the control group, but there was no significant difference between the two groups (P>0.05). Conclusion: For infants at high risk of cerebral palsy, ultra-early diagnosis and intervention can improve the normalization rate of high-risk infants with cerebral palsy and reduce the incidence of cerebral palsy which is of significant clinical benefit. 2021 Vol. 51 (4): 298-302 [Abstract] ( 655 ) HTML (1 KB)  PDF (1426 KB)  ( 602 ) 303 Social anxiety and its influencing factors in children with acute lymphoblastic leukemia Zhou Honghua, Zhang Chunmei, Shen Xiaowen, Zheng Xiaofen, Wu Yamei, Ying Jinhong DOI: 10.3969/j.issn.2095-9400.2021.04.009 Objective: To investigate the social anxiety level and its influencing factors in children with acute lymphoblastic leukemia, and to supply theoretical basis for clinical nursing. Methods: Totally 104 children with ALL who were hospitalized in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University and the Affiliated Children’s Hospital of Zhejiang University School of Medicine from December 2018 to September 2019 were selected as research subjects by convenience sampling method. Social anxiety scale for children and pediatric quality of life inventory 4.0 generic core scale were used for the investigation. Data were analyzed by nonparametric test, multivariate linear regression and Spearman method. Results: The incidence of social anxiety in children was 48.08%, with the mean score of social anxiety being 7(2, 10). Multiple liner regression showed the major influencing factors (P<0.05) were induction remission period, 13-18 years of age, democratic parenting style and screen time. The social anxiety level was negatively correlated with quality of life (P<0.05). Conclusion: Children with acute lymphoblastic leukemia have a higher incidence of social anxiety, which affects their quality of life. We should pay more attention to the induction remission stage, encourage the teenagers to reduce screen time and advocate democratic parenting. 2021 Vol. 51 (4): 303-306 [Abstract] ( 890 ) HTML (1 KB)  PDF (1448 KB)  ( 978 ) 307 The correlation between cognitive dysfunction and MTHFR gene polymorphism in children with acute lymphoblastic leukemia HUANG Zhen, HU Linglong, WANG Yao, HUANG Tingting, CHEN Min, WANG Juxiang, ZHOU Haixia, LI Yuan. DOI: 10.3969/j.issn.2095-9400.2021.04.010 Objective: To investigate the relationship between methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and plasma homocysteine (Hcy) and cognitive function in children with acute lymphoblastic leukemia (ALL). Methods: 39 children with acute lymphoblastic leukemia who were hospitalized in the Department of Hematology of the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from January 2015 to December 2019 were selected. Polymerase chain reaction-restriction enzyme fragment and length polymorphism analysis (PCR-RFLP) was used to detect the MTHFR genotype. The Wechsler Children’s Intelligence Scale Fourth Edition (WISC-IV) was used to assess cognitive function. Hcy level was also measured to analyze MTHFR. The correlation of gene polymorphism with Hcy level and cognitive function was analyzed by Pearson correlation. Results: Three genotypes existed in the C677T locus of the MTHFR gene in children with acute lymphoblastic leukemia. Compared with each genotype, Hcy level in the TT genotype was higher than that in the CT and CC types (P<0.05). There was a significant negative correlation between serum Hcy level and cognitive function, especially the operational IQ (PIQ) in children with ALL. MTHFR 677 C→T gene mutation was negatively correlated with ALL cognitive function (FIQ: r=-0.806, VIQ: r=-0.735, PIQ: r=-0.802, P<0.001). Conclusion: The MTHFR C677 T gene mutation, which may be a genetic susceptibility factor for cognitive impairment, can increase plasma Hcy levels and then affect cognitive function. 2021 Vol. 51 (4): 307-310 [Abstract] ( 600 ) HTML (1 KB)  PDF (1458 KB)  ( 638 ) 311 The role of Schisandrin B in inhibiting proliferation of gefitinib-resistant lung cancer cells via down-regulation of IGFBP2 expression SUN Lei, LAN Xiu, LYU Zhuqing, LI Weiwen. DOI: 10.3969/j.issn.2095-9400.2021.04.011 Objective: To investigate the role of Schisandrin B (Sch B) in inhibiting proliferation of gefitinib (Gef) resistant lung cancer cells and its molecular mechanism. Methods: PC9/GR (Gefitinib resistant PC9 cells) resistant cells were domesticated with gradient concentration of Gef and maintained with 200 mmol/L Gef. The difference of IGFBP2 expression and the viability between PC9 and PC9/GR cells were analyzed. The viability, apoptosis, IGFBP2 expression and phosphorylation of AKT and mTORC1were measured with and without Sch B treatment. Lentivirus transfection to obtain IGFBP2 overexpression (OE) cells was applied to confirm the role of IGFBP2 on proliferation inhibition of Sch B in PC9/GR cells. Results: The expression of IGFBP2 in PC9/GR cells was higher than that in PC9 cells (P<0.05). After Sch B treatment, the survival rate, IGFBP2 expression and phosphorylation of AKT and mTORC1 decreased (P<0.05), whereas the apoptosis increased (P<0.05). The overexpression of IGFBP2 increased the phosphorylation of AKT and mTORC1 but alleviated the proliferation inhibition of Sch B in PC9/GR cells (P<0.05). Conclusion: Sch B induces proliferation inhibition via down-regulating expression of IGFBP2 in PC9/GR cells. 2021 Vol. 51 (4): 311-314,318 [Abstract] ( 624 ) HTML (1 KB)  PDF (1645 KB)  ( 579 ) 315 Promotion of romotes skeletal muscle function recovery in hysterectomy patients by Shenmai injection ZHU Meizhen, PAN Yafei, CHEN Lei, WANG Liangrong, LIN Li’na DOI: 10.3969/j.issn.2095-9400.2021.04.012 Objective: To investigate the effect of Shenmai injection pretreatment on the levels of cytokines in abdominal drainage and skeletal muscle function in patients undergoing laparoscopic hysterectomy. Methods: Sixty-six patients undergoing laparoscopic hysterectomy were randomly divided as the control group (C group) and the Shenmai group (SM group). The patients in SM group received intravenous administration of Shenmai injection 0.6 mL/kg before skin incision, while the same amount of saline was given instead in C Group. The grip strength, muscle endurance, and inflammatory cytokines levels in abdominal drainage fluid were measured before surgery as baselines and 4 h, 1, 2 days post-operation as well. The occurrence of nausea, vomiting and other adverse events were also documented. Results: Compared with C group, the declines in grip strength and muscle endurance in SM group were significantly attenuated in each time point (P<0.05). However, grip strength and muscle endurance were decreased in both groups, compared with baseline values (P<0.05). The levels of IL-6 and TNF-α in the abdominal drainage fluid of SM group at 4 hours post-operation were significantly lower than those of group C (P<0.05), and the level of IL-6 in the drainage fluid was negatively correlated with grip work (P<0.05). There was no significant difference in adverse events between the the groups (P>0.05). Conclusion: Pretreatment with Shenmai injection 0.6 mL/kg could reduce the production of inflammatory cytokines and thus promote the recovery of skeletal muscle function in patients undergoing laparoscopic hysterectomy. 2021 Vol. 51 (4): 315-318 [Abstract] ( 637 ) HTML (1 KB)  PDF (1416 KB)  ( 553 ) 319 The effectiveness of vitamin D on respiratory distress syndrome of premature infants HOU Qiuying, LIU Jue, ZHANG Xu, TENG Yiqun. DOI: 10.3969/j.issn.2095-9400.2021.04.013 Objective: To study the effectiveness of vitamin D on neonatal respiratory distress syndrome (NRDS). Methods: Totally 82 cases of premature infants (28 weeks < gestational age < 34 weeks) with NRDS from Jiaxing Second Hospital from March 2017 to March 2019 were selected as NRDS group for prospective study, which was further divided randomly as subgroup (routine treatment) and subgroup 2 (routine treatment plus oral vitamin D 1 000 IU/d). Another 84 preterm infants without NRDS in the same period were selected as the control group for routine treatment only. Vitamin D level was compared at birth and its effect on NRDS in two subgroups were evaluated. Results: Vitamin D level in NRDS group was significantly lower than that in the control group (P<0.05). The NRDS group had lower Downes respiratory distress score and PaCO2 level, shorter hospital stay and lower complication rate (P<0.05). Conclusion: Vitamin D is generally deficient in preterm infants, especially in NRDS. 1 000 IU/d vitamin D as an adjuvant treatment can significantly reduce the disease severity, oxygen use time, hospital stay and complications of NRDS in preterm infants. 2021 Vol. 51 (4): 319-322 [Abstract] ( 555 ) HTML (1 KB)  PDF (1396 KB)  ( 579 ) 323 The influence of original family cohesion and adaptability on the puerperant postpartum depression ZHANG Mingzhe, GONG Xinyue, LI Wenjia, CHEN Shenmin, YAN Qiaoyi, DONG Chaoqun. DOI: 10.3969/j.issn.2095-9400.2021.04.014 Objective: To investigate the puerperant postpartum depression (PPD) within 3 years after childbirth, and to explore the impact of original family cohesion and adaptability on PPD. Methods: From July to August 2020, a convenient sample of 211 puerperants within 0-3 years postpartum were investigated online using the Edinburgh postnatal depression scale and Family Adaption and Cohesion Evaluation Scale-Chinese Version. Results: PPD within 3 years after childbirth was found in 34.1% puerperants. Puerperants from families with loose family cohesion, rigid family adaptability and extreme family type had higher scores of postpartum depression. After controlling for the demographic variables, the multiple linear regression analysis revealed that original family cohesion could negatively predict the degree of postpartum depression (β=-0.175, P=0.013), while the extreme type of family could positively predict the degree of postpartum depression (β=0.178, P=0.033). Conclusion: The prevalence of postpartum depression is relatively high within 3 years after childbirth. Original family cohesion and family type are important influencing factors of postpartum depression. Clinical medical staff might as well carry out postpartum depression prevention and intervention program from the perspective of the original family. 2021 Vol. 51 (4): 323-326,329 [Abstract] ( 883 ) HTML (1 KB)  PDF (1516 KB)  ( 758 )

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