温州医科大学学报
 
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2020 Vol. 50, No. 10
Published: 2020-10-25
 
 
775 The expression and localization of DAXX in gastric cancer and the effect of its SUMO on the malignant phenotype of gastric cancer
CHEN Chenbin, HUANG Qunjia, XIE Wangkai, XU Jianfeng, SHEN Xian,Xue Xiangyang,Zhao Zhiguang
DOI: 10.3969/j.issn.2095-9400.2020.10.001
Objective: To explore the expression and localization of DAXX in gastric cancer and the effect of its SUMO-induced DAXX on the malignant phenotype of gastric cancer cells. Methods: The expression and prognosis of DAXX in gastric cancer from TCGA database were analyzed. Real-time quantitative PCR (RT-qPCR) was used to detect the mRNA level of DAXX in gastric cancer cell lines. Samples of gastric cancer and the adjacent tissue were collected from 20 patients after surgery between January and June 2017 in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University. The expression and localization of DAXX were detected by immunohistochemistry. CCK-8 and wound healing assays were used to examine the effect of DAXX on cell proliferation and migration, respectively. Western blot was used to detect the distribution of DAXX and its correlation with SUMO-2/3.Results: Bioinformatics analysis showed that the expression level of
DAXX in gastric cancer was higher than that in adjacent normal tissues, but had no effect on the survival prognosis of patients (P=0.52). The immunohistochemical results showed that the positive rate of DAXX expression in gastric cancer tissues (65.0%) was higher than that in cancer tissues (50%). And DAXX was mainly expressed in the nuclear. RT-qPCR confirmed that the mRNA expression level of DAXX in gastric cancer cell lines was significantly increased. The results of CCK-8 assay indicated that DAXX significantly inhibited cell proliferation(P<0.05), while the wound healing assay indicated that DAXX significantly promoted cell migration (P<0.01).Western blot showed differences in the distribution of DAXX and there was a significant SUMO-2/3 modification in DAXX. Conclusion: DAXX is highly expressed in gastric cancer and is mainly localized in the nucleus. DAXX may affect nuclear-plasma transport by inhibiting gastric cancer cell proliferation and promoting metastasis via interaction with SUMO2/3.
2020 Vol. 50 (10): 775-781 [Abstract] ( 637 ) HTML (1 KB)  PDF (2338 KB)  ( 742 )
782 The value of fluorescent imaging by c(RGDyk) modified liposome encapsulating quantum dots in the accurate delineation of glioma margin for the precise surgical resection of tumor
WU Zhaohang,DONG Weijie, XUE Pengpeng, ZUO Jiayi, WANG Lifen, YUAN Jiandong, XU Helin
DOI: 10.3969/j.issn.2095-9400.2020.10.002
Objective: To prepare c(RGDyk) cyclic peptide modified liposome encapsulating quantumdots (QDs) and evaluate its ability to delineate accurately glioma margin by fluorescent imaging for the accurate urgical resection of tumor. Methods: The mixed lipid materials including hydrogenated soybean phospholipid/distearoyl phosphoethanolamine-PEG2000/cholesterol were used to encapsulate ZnCdSe/ZnS quantum dots(QDs) to prepare QDs-liposome (QDs-Lip) through the reverse evaporation method. Afterwards, c(RGDyk) cyclic peptide as a targeting ligand was conjugated to the amino group on the surface of QDs-Lip by EDC/NHS
condensation reaction to obtain QDs-c(RGDyk)-Lip. Dynamic light scatting (DLS) and Transmission electron microscope (TEM) were used to characterize particle size and morphology of QDs-c(RGDyk)-Lip in vitro.Meanwhile, the stability of QDs-c(RGDyk)-Lip in physiologic relevant medium was also evaluated in terms of both fluorescent emission spectrum and particle size. In vitro CCK-8 assay was used to evaluate the toxic effect of QDs-c(RGDyk)-Lip on PC12 cells and C6 cells. Besides, the cellular uptake of QDs-c(RGDyk)-Lip by C6 cells was evaluated by laser scanning confocal microscopy in presence of free c(RGDyk) or not. Upon intravenous
injection of QDs-c(RGDyk)-Lip, fluorescent imaging of brain of glioma-bearing rats was collected to evaluate its ability to accurately delineate the margin between tumor tissue and normal tissue and the accurate surgical resection of tumor was confirmed by HE staining. Results: The successful encapsulation of QDs was confirmed
by TEM and DLS. The particle size of free QDs was about 35nm, but the particle size of QDs-c(RGDyk)-Lip increased to 175 nm with narrow particle distribution (PDI=0.12). Moreover, QDs-c(RGDyk)-Lip displayed the vesicle-like morphology with small aggregate of QDs in center. QDs-c(RGDyk)-Lip exhibited the maximum
peak at 610 nm by the fluorescent emission spectrum. The good stability of QDs-c(RGDyk)-Lip in in physiologic relevant medium was confirmed by both fluorescent emission spectrum and particle size. In vitro cytotoxicity study showed that QDs-c(RGDyk)-Lip had the weak toxicity toward PC12 or C6 cells only at high concentration.
QDs-c(RGDyk)-Lip was effectively up-taken by C6 cells, resulting in the strong fluorescence in their cytoplasm.Moreover, the fluorescence of QDs-c(RGDyk)-Lip in C6 cells was obviously inhibited by pre-incubating with free c(RGDyk), indicating that its specific targeting toward C6 cells. Animal experiments show that QDsc(
RGDyk)-Lip can effectively target the glioma-bearing rats after intravenous injection for the accurate surgical resection of tumor. Conclusion: c(RGDyk) cyclic peptide modified liposome in combination with quantum dots constructs QDs-c(RGDyk)-Lip as a safe and stable fluorescent probe, which can specifically target glioma tissue
and accurately guide surgical resection.
2020 Vol. 50 (10): 782-788 [Abstract] ( 703 ) HTML (1 KB)  PDF (1893 KB)  ( 761 )
789 Metabolic alterations in the contralateral cerebellum of rats with transient and permanent middle cerebral artery occlusion
XIA Nengzhi, HU Zilong, HUANG Qun, ZHENG Hong, GAO Hongchang, YANG Yunjun,LI Jiance.
DOI: 10.3969/j.issn.2095-9400.2020.10.003
Objective: To investigate metabolic alterations in contralateral cerebellum of the transient(tMCAO) and permanent middle cerebral artery occlusion (pMCAO) rats by 1H NMR-based metabolomics.Methods: The rats were divided into CON group (n=12), pMCAO group (n=29) and tMCAO group (n=23). Brain MRI scan was performed and the contralateral cerebellum was detected by 1H NMR with pattern recognition and quantitative analysis of metabolites. Results: Although MRI showed no obvious abnormalities, the metabolic patterns of contralateral cerebellum were significantly changed in three groups. Compared with CON group,alanine (Ala) and lactate (Lac) decreased significantly, while γ-aminobutyric acid (GABA), glutamine (Gln),myo-inositol (m-Ins), aspartate (Asp) and creatine (Cre) increased significantly for contralateral cerebellum in pMCAO group (P<0.05); Ala and Lac decreased significantly, while glutamate (Glu), m-Ins, Asp and Cre increased significantly for contralateral cerebellum in tMCAO group (P<0.05). Gln of contralateral cerebellum decreased significantly in tMCAO group compared with pMCAO group (P<0.05). Conclusion: The metabolism of the contralateral cerebellum was significantly altered in the transient and permanent cerebral ischemia, mainly involving energy metabolism, neurotransmitter metabolism and glial cell activity. Different ischemic time showed variant impact on Gln-Glu-GABA cycle of cerebellum.
2020 Vol. 50 (10): 789-793,799 [Abstract] ( 642 ) HTML (1 KB)  PDF (2663 KB)  ( 679 )
794 Changes in expression of circular RNA-42398 in liver fibrosis model and its biological function
LYU Xueyou, ZHOU Yuping, YANG Ping, ZHU Linwen.
DOI: 10.3969/j.issn.2095-9400.2020.10.004
Objective: To determine the changes of mmu_circ_42398 expression in the liver tissue from a murine model of liver fibrosis and cell culture models that reproduce liver disease, and make an initial exploration into its biological function. Methods: A mouse model of liver fibrosis was prepared and three murine cell culture models were reproduced: a hepatic stellate cell (JS1) activation model, a hepatocyte (AML12) oxidative stress injury model, and a macrophage inflammation (RAW264.7) model. A normal control group was also established.qPCR was used to measure the expression of mmu_circ_42398 in the liver tissue and in cells from the above cell culture models, and bioinformatic analysis was used to predict the circRNA target gene, to analyze its biological function, and to map its competing endogenous RNA (ceRNA) signaling pathway. The mmu_circ_42398 over expression vector was constructed and produced and then transfected into JS1 cells to examine the protein expression of a-SMA, ColI. Results: Compared with the control group, the expression of mmu_circ_42398was significantly decreased in liver tissue from the mouse liver fibrosis model (P<0.05), and its expression was significantly decreased in all three cell culture models. Bioinformatic analysis predicted that mmu_circ_42398could affect the activation of hepatic stellate cells by binding to the target genes miR-338-3p and miR-344-3p.The over expression of mmu_circ_42398 in JS1 cells resulted in decreased of protein expression of a-SMA, ColI.Conclusion: The expression of mmu_circ_42398 is significantly changed in the liver fibrosis model and may be
involved in the activation of hepatic stellate cells.
2020 Vol. 50 (10): 794-799 [Abstract] ( 605 ) HTML (1 KB)  PDF (2183 KB)  ( 605 )
800 The MRI features and pathology of intrahepatic mass-forming cholangiocarcinoma with rich arterial blood supply
XIAO Yangrui, MAO Weibo, LI Bingrong, WANG Zufei.
DOI: 10.3969/j.issn.2095-9400.2020.10.005
Objective: To investigate the MRI features and pathology of intrahepatic mass-forming cholangiocarcinoma (IMCC) with rich arterial blood supply. Methods: A retrospective analysis was made of the initial-diagnosis MR data of IMCC patients pathologically confirmed in Lishui Central Hospital from November 2008 to July 2019. Totally 14 lesions (in 14 IMCC patients) showing extensive enhancement of arterial phase were included in the study, and their imaging characteristics, including the general condition of the lesion, the signal on plain scan and DWI, the enhancement pattern and accompanying signs of surrounding tissues were analyzed. Results: The MICC with rich arterial blood supply mostly demonstrated as nodule or small mass, with regular morphology and clear boundary; the T1WI was relatively uniform low signal; while the T2WI and DWI were uniform or uneven high signal. There were three enhancement patterns: Type I, 9 cases (9/14), demonstrated whole or nearly whole enhancement in the arterial phase and continuously stable enhancement in the later period;Type II, 4 cases (4/14), demonstrated thick ring-enhancement in the arterial phase and continuously stable enhancement in the later period; Type III, 1 case (1/14), demonstrated overall enhancement in the arterial phase and quick washout in the portal venous phase. Otherwise, 7 cases (7/14) had liver fibrosis background; 6 cases(6/14) showed transient abnormal perfusion enhancement around the neoplasm in the arterial phase; 6 cases (6/14)showed pseudocapsule with delayed enhancement. Overall, no cases showed obvious steatosis, hemorrhage,bile duct dilatation or hepatic envelope depression. Dense distribution of tumor cells, abundant vascular fissures,yet loose distribution of fibrous stroma, rare coagulation necrosis and hemorrhage were the pathological fea-tures of IMCC with rich arterial blood supply. Conclusion: IMCC with rich arterial blood supply demonstrated specific MRI features of its own, which is of clinical value.
2020 Vol. 50 (10): 800-804 [Abstract] ( 671 ) HTML (1 KB)  PDF (4395 KB)  ( 828 )
805 Cryptotanshinone enhances the chemosensitivity of gemcitabine in cholangiocarcinoma
ZHOU Gongting,WANG Jisheng, Chen Yiming, HONG Zhong, Liao Yi.
DOI: 10.3969/j.issn.2095-9400.2020.10.006
Objective: To investigate the effect and mechanism of cryptotanshinone on the chemosensitivity of QBC939 cells to gemcitabine. Methods: The human cholangiocarcinoma QBC939 cells were divided into four groups: control group, cryptotanshinone group, gemcitabine group and cryptotanshinone+gemcitabine group.
CCK-8 method was used to detect the inhibition rate of cell growth. Apoptosis was detected by flow cytometry.Western blot was used to detect the expression of apoptosis related protein. Results: After 24 h treatment, when the final concentration of cryptotanshinone was 10, 30 and 50 μmol/L, the inhibition rate of cell growth was
22.30%±2.15%, 42.53%±3.62% and 51.85%±4.81% respectively, which was lower than that of the control group(99.22%±3.52%), with statistical difference (P<0.05). After 24 h treatment, the apoptosis rate of cryptotanshinone+gemcitabine combination group was 35.40%±1.57%, which was higher than the cryptotanshinone group
(19.80%±0.92%), the gemcitabine group (24.20%±0.56%) and the control group (8.84%±0.58%). The difference was statistically significant (P<0.05). Western blot showed that the expression of active caspase-3 and Bax protein in the combination of cryptotanshinone and gemcitabine group was higher than that in gemcitabine group,cryptotanshinone group and control group. Conclusion: Cryptotanshinone can increase the chemosensitivity of gemcitabine in cholangiocarcinoma cells.
2020 Vol. 50 (10): 805-809 [Abstract] ( 661 ) HTML (1 KB)  PDF (1921 KB)  ( 604 )
810 A study on the characteristics of attentional bias in anxiety disorders and its correlation with cognitive styles and personality traits
LIN Zijiang, CHEN Li, WANG Changlyu, YANG Chuang, XIE Linshan,ZHANG Jiaying.
DOI: 10.3969/j.issn.2095-9400.2020.10.007
Objective: To investigate the characteristics of the negative attentional bias in patients with anxiety disorder and explore the correlation among attention bias, cognitive styles and personality traits. Methods:Attentional bias, cognitive styles and the personality traits were assessed by the dot-probe paradigm and the psychology scales in 36 anxiety disorder patients and 36 normal people. Scores from the dot-probe paradigm and the psychology scales were compared between the anxiety disorder patients and the normal people in the attention bias, cognitive styles and personality traits. The scores from the dot-probe paradigm were also used to examine the differences in the attention bias of the men versus women, the social anxiety disorder versus the generalized anxiety disorder. The correlation among attention bias, cognitive styles and personality traits was analyzed.Results: There was significant difference in the negative attentional bias scores, the facilitated attention scores
between the anxiety and the control groups (P<0.05), but no significant difference in the accuracy scores or the disengaging attention scores between them (P>0.05). There was significant difference in the scores of automatic hinking, dysfunctional attitude and Eysenck personality Questionnaire’s N dimension between the anxiety andthe control groups (P<0.05). There was significant difference in disengaging attention scores between the male nd female (P<0.05). There was no significant difference in the negative attentional bias scores, the facilitated ttention scores, the disengaging attentional bias, the accuracy scores between the social anxiety disorder and eneralized anxiety disorder (P>0.05). There was significant correlation between the dysfunctional attitude cores, the negative attentional bias scores (r=0.383, P<0.05) and the facilitated attention scores (r=0.204,P<0.05). There was no significant correlation among the negative attentional bias scores, the facilitated attention cores, the automatic thinking and the N dimension scores of Eysenck Personality Questionnaire (P>0.05). There as no significant correlation among the accuracy scores, the disengaging attention scores, the automatic thinking uestionnaire scores, the dysfunctional attitude scores, and the Eysenck N dimension scores (P>0.05). Conclusion:Patients with anxiety disorder have attentional bias to negative stimulus, manifested by attention alert to negative timulus. The negative attentional bias has gender difference, which is related to cognitive styles.
2020 Vol. 50 (10): 810-814 [Abstract] ( 682 ) HTML (1 KB)  PDF (1372 KB)  ( 881 )
815 Preparation of acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold with sustained release of TGF-β3
HE Linjie, LIAO Xin, PAN Yinan, HUANG Yixing.
DOI: 10.3969/j.issn.2095-9400.2020.10.008
Objective: To explore the methods for preparation of acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold which is injectable and can release TGF-β3 sustainedly. Methods:Decellularized cartilage microfilaments with sustained release of TGF-β3 were prepared by the method of detergent-enzyme chemical digestion and layer-by-layer self-assembly technology. After being mixed with thermosensitive hydrogel, the acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold was prepared. The sustained release of TGF-β3 was detected by ELISA kit. Then the bone marrow mesenchymal stem cells (BMSCs) were co-cultured with the composite scaffold and observed under microscope. The expression of Col II, AGG and Sox9 were detected by the method of Western blot. Results: The composite scaffold was in sol state at low temperature, and changed to gel state when the temperature increased to 37 ℃. The TGF-β3 in the composite scaffold could be released slowly and effectively for about 25 d. BMSCs grew well on the composite scaffold, and the cells were similar to chondrocytes. And the expression of Col II, AGG and SOX9 increased significantly (P<0.05). Conclusion: The acellular cartilage microfilaments/thermosensitive hydrogel composite scaffold, injectable and able to release TGF-β3 sustainedly, was successfully prepared, which can induce chondrogenic differentiation of BMSCs.
2020 Vol. 50 (10): 815-819 [Abstract] ( 651 ) HTML (1 KB)  PDF (1642 KB)  ( 658 )
820 Diagnostic value and correlation analysis of parameters derived from two-compartment exchange model and IVIM and models for uterine cervical carcinoma
WANG Xue, LU Yi, SONG Jiao, LIN Wenxiao, YAN Zhihan
DOI: 10.3969/j.issn.2095-9400.2020.10.009
Objective: To analyze the diagnostic value of parameters as well as the correlation of perfusion parameters derived from CC (two-compartment exchange model) and IVIM (intravoxel incoherent motion)models for uterine cervical carcinoma. Methods: Thirty-two patients diagnosed with uterine cervical carcinoma pathologically and 14 healthy subjects were included in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from April 2016 to December 2018. MRI examinations including dynamic contrasted-enhanced MRI (DCE-MRI) and IVIM sequences were performed for all the subjects before treatment. Parameters derived from CC model were listed as follows: tissue blood flow (Fp), tissue blood volume (Vp), interstitial volume (Ve), vascular permeability (PS). Parameters derived from IVIM model were listed: true diffusion coefficient (D), pseudo diffusion coefficient (D*), fraction of fast apparent diffusion coefficient (f) and fD*. ROC test was used to compare the corresponding AUC (the area under the curve) value subsequent to the comparsion of the differences of those parameters from CC and IVIM models between cervix carcinoma and normal cervix tissue. Spearman’s correlation was applied to analyze the perfusion parameters between CC and IVIM models from cervical carcinoma and normal cervix tissue. Results: The differences between the parameters of CC model (Vp, Ve, PS) in cervical carcinoma mass and normal cervix tissue were statistically significant(P<0.05). The AUC of the parameters of CC model was 0.546-0.852 while the AUC of CC-PS and CC-Ve werere spectively >0.8 and >0.7. The differencess of IVIM parameters (D, D*, f, fD*) between cervical carcinoma mass and normal cervix tissue were statistically significant (P<0.05). The AUC of each parameter of IVIM was0.642-0.953, in which the AUC of IVIM-D, IVIM-f and IVIM-fD* were >0.8. In cervical carcinoma, IVIM-f and IVIM-fD* were slightly correlated with CC-Ve (0.3< r≤0.5). Conclusion: Evaluating and identifying cervical carcinoma with the quantitative parameters of CC and IVIM models were found to be valuable predictors. The perfusion parameters from IVIM model of cervical carcinoma have the potential to be used as a non-invasive method to evaluate the microcirculation status of the tumor.
2020 Vol. 50 (10): 820-824 [Abstract] ( 631 ) HTML (1 KB)  PDF (1435 KB)  ( 587 )
825 The efficacy of combined vitrectomy-lensectomy and non-combined surgery in the treatment of proliferative diabetic retinopathy
HU Zhixiang, LIN Haishuang, YE Lingying, XU Mingna, LIN Ke, CHEN Tianyu,WU Ronghan.
DOI: 10.3969/j.issn.2095-9400.2020.10.010
Objective: To analyze the effect of combined vitrectomy-lensectomy surgery and non-combined surgery assisted by wide-angle viewing system in the treatment of proliferative diabetic retinopathy (PDR) with cataract. Methods: A retrospective study was conducted in which patients with PDR who underwent vitrectomy assisted by wide-angle viewing system from January 2014 to February 2018. The patients were divided into combined operation group and non-combined operation group according to whether combined lensectomy was used in vitrectomy. The visual acuity changes, total operation time and complications were compared between the two groups. Results: A total of 76 patients (98 eyes) with PDR were enrolled in this study. 57 eyes underwent combined surgery and 41 eyes underwent non-combined surgery. When the preoperative visual acuity was better than finger count, the logMAR visual acuity of the combined operation group improved more than that of the
non-combined operation group (0.6 vs. 0.3, Z=-1.991, P=0.047). Among 39 patients (51 eyes) who were not filled with silicone oil during the initial operation, the total operation time of the combined operation group was shorter than that of the non-combined operation group (Z=-2.368, P=0.018). The common complications of
vitrectomy and silicone oil tamponade in PDR patients were high intraocular pressure and re-bleeding of fundus.Conclusion: For patients with preoperative visual acuity better than finger count, it is recommended to combine phacoemulsification with intraocular lens implantation in PDR vitrectomy to reduce operation time and obtain
better visual acuity.
2020 Vol. 50 (10): 825-829 [Abstract] ( 640 ) HTML (1 KB)  PDF (1376 KB)  ( 785 )
830 Expression of programmed-death ligand 1 in upper tract urothelial carcinoma and its prognostic significance
SUN Yaorong, CHEN Hui, YAN Huihui, Lin Li.
DOI: 10.3969/j.issn.2095-9400.2020.10.011

Objective: To analyze the relationship between the expression of programmed-death ligand1 (PD-L1) and the clinicopathological features and prognosis of upper tract urothelial carcinoma (UTUC).Postoperative follow-up was performed to evaluate the prognosis of the patients, and to analyze the correlation between the expression of PD-L1 and the prognosis of the patients. Methods: Immunohistochemistry (IHC) was used to detect the expression of PD-L1 in 151 patients with UTUC radical nephroureterectomy (RNU). Results:The median age of the patients was 52 years old (range: 36-74 years old). The median follow-up time was 63months (range: 1-100 months), and the expression of PD-L1 was negative in 50 (33%) of the samples. Negative PD-L1 expression was significantly associated with higher tumor pathologic stage (P=0.04), higher lesion grade(P=0.01), and lymphovascular invasion (P<0.01). Kaplan-Meier survival curve analysis showed that PD-L1 negative expression was associated with poor tumor-free survival (P=0.01) and poor cancer-specific survival(P=0.01). Multivariate Cox regression analysis showed that negative PD-L1 expression was significant associated with disease recurrence (HR=2.05, 95%CI=1.06-3.96, P=0.032) and cancer-specific mortality (HR=2.89, 95%CI=
1.22-6.82, P=0.024), after adjusting for tumor pathological stage, lesion grade, lymph node metastasis, and lymphovascular invasion. Conclusion: Negative PD-L1 expression is an independent predictor of cancer recurrence and tumor-specific survival after UTUC radical nephroureterectomy, indicating the necessity of
incorporating UTUC patients into immunotherapy clinical trials.
2020 Vol. 50 (10): 830-833,838 [Abstract] ( 542 ) HTML (1 KB)  PDF (1526 KB)  ( 643 )
834 The effect of hemolysis on 27 biochemical items tested in automatic biochem analyzer of ADVIA 2400 of SIEMENS and correcting analysis
NI Li, YE Yuedi, XU Xiaojie, GE Haifeng, CHEN Yunguo, ZHU Lidan.
DOI: 10.3969/j.issn.2095-9400.2020.10.012
Objective: To explore the effect of hemolysis on the results of important biochemical items by hemolysis index (H). Methods: Referring to the standard procedure of the EP7-A2 file of the evaluation program formulated by the CLSI, 27 biochemical items and H of 40 samples were detected at automatic biochem analyzer of ADVIA 2400 of SIEMENS. The 40 samples were averagely divided as 4 groups whose hemolysis were +, ++, +++ and ++++ respectively after their RBC were crushed by tallies. The 27 items and H of 40 samples were tested after hemolysis. The bias of the results of 27 items after hemolysis were compared with the corresponding TEa of tests of CLIA’88. △H was taken for X axis and the corresponding interference value of every test was taken for Y axis, and the interference curves were drawn. Results: Hemolysis had no obvious interference on the 21 biochemical items, including DBil, TBil, PA, TP, TG, UA, Alb, ALP, ALT, BUN, ChE, TC, Cr, GGT, HDL, LDL, Ca, P, Mg, Na+ and Cl-. There were obvious positive bias to AST, K+ and LDH when hemolysis was +, and there was obvious negative bias of Glu when hemolysis was +. There was obvious positive bias to CK when hemolysis was ++ and there existed obvious positive bias to Fe when hemolysis was +++. Conclusion: There are obvious bias to Glu, AST, K+ and LDH when hemolysis is +, which are tested at automatic biochem analyzer of ADVIA 2400 of SIEMENS. If new samples can not be collected, the results of the items which are interfered obviously should be corrected according to corresponding hemolysis indexes before report.
2020 Vol. 50 (10): 834-838 [Abstract] ( 539 ) HTML (1 KB)  PDF (1545 KB)  ( 664 )
839 Clinical features and peripheral hemogram analysis of 309 first-diagnosed acute leukemia patients
QU Xiaoxiao, ZHANG Yanhong, LI Mianmian, JIANG Weiyan, YU Lingling.
DOI: 10.3969/j.issn.2095-9400.2020.10.013
Objective: To provide a new basis for the diagnosis and treatment of acute leukemia (AL) by analyzing the clinical symptoms of first-diagnosed AL patients and the characteristics of peripheral blood changes.Methods: The clinical data, peripheral blood features and diagnostic classification on first-diagnosed AL patients were retrospectively analyzed, who were admitted and diagnosed by the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from January 2014 to December 2018. Results: Most patients with AL had one or more typical clinical symptoms, of which anemia (53.07%) and hemorrhage (42.39%) were the most common, and more and more patients with only one or non-typical clinical symptoms (37.86% and13.27% respectively). There was significant difference in the detection rate of blasts in blood smear microscopy between different white blood cell (WBC) levels (P<0.01). With the increase of WBC counts, the detection rate of blasts increased significantly. There were no significant differences in WBC counts and the detection rate of blasts between different clinical symptoms (P>0.05), but there was significant difference between hemoglobin(HGB) content and platelet (PLT) count (P<0.05). Conclusion: The non-specific clinical manifestations of AL are
increased. Patients with atypical clinical symptoms, whose changes in blood routine examination are not obvious,should be highly alert to the possibility of AL. As patients with normal or reduced WBC are easily ignored,peripheral blood morphological examination should be strengthened.
2020 Vol. 50 (10): 839-843 [Abstract] ( 586 ) HTML (1 KB)  PDF (1403 KB)  ( 902 )
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