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2020 Vol. 50, No. 8 Published: 2020-08-25   603 The study to the mouse models of hepatic non-alcoholic steatofibrosis ZHOU Yuping, WEN Jinfeng, ZHOU Fei, WANG Qingling, YANG Ping, LYU Xueyou DOI: 10.3969/j.issn.2095-9400.2020.08.001 Objective: To establish a hepatic non-alcoholic steatofibrosis (NASF) mouse model by the combination of methionine-choline-deficient diet (MCD) and repeated injection of carbon tetrachloride (CCl4). Methods: C57BL/6J mice were randomly divided into the control group and the model group. The model mice were fed with MCD, and intraperitoneally injected by 10% CCl4 once a week (2 mL/kg body weight), while the control group was fed with methionine-choline-supplemented diet (MCS) only. When all mice were killed at 4th and 6th weeksrespectively, the serum and the liver were collected. The histological analysis was conducted by HE staining, Oil Red O staining and Masson staining. The hepatic alpha smooth muscle ctin (α-SMA) protein expression was detected by immunohistochemistry. In addition, serum liver function and liver triglyceride levels were measured. Results: Compared with the control group, model group mice were significantly increased (P<0.01) in the activities of ALT, AST and TBIL in serum and the Triglyceride content in the liver. The liver tissue in model group mice showed obvious inflammation, steatosis and fibrosis. The α-SMA protein expression markedly increased, compared with the control group. Moreover, the pathological changes in the 6-week were more serious than those in the 4-week model mice. Conclusion: The NASF animal model can be established successfully by the combination of MCD and repeated injection of CCl4. 2020 Vol. 50 (8): 603-608 [Abstract] ( 998 ) HTML (1 KB)  PDF (1726 KB)  ( 965 ) 609 Effect of miR-19a on proliferation and migration of vascular smooth muscle cells and inflammatory infiltration in abdominal aortic aneurysm OUYANG Yong, LIU Yan, YE Yangbo DOI: 10.3969/j.issn.2095-9400.2020.08.002 Objective: To investigate the effect of miR-19a on proliferation and migration of vascular smooth muscle cells and inflammatory infiltration through targeting CDKN2B in abdominal aortic aneurysm (AAA) and its underlying mechanism. Methods: Serum samples were collected from 15 patients without any treatment after primary AAA, 15 healthy persons during the same period, and samples of AAA tissues and without AAA tissues were collected from 15 patients. RT-qPCR was used to detect the expression of miR-19a. CCK-8, Transwell, and flow cytometry were respectively performed to examine the proliferation, migration and apoptosis of vascular smooth muscle cells. Inflammatory cytokines secretion of THP-1 cells were evaluated using enzyme-linked immunosorbent assay (ELISA). The expression of protein was measured by western blot. Dual-luciferase reporter gene assay was applied to verify the interaction relationship between miR-19a and CDKN2B. Results: Compared with the control group, miR-19a was significantly overexpressed in the serum samples and tissues of AAA patients. Knockdown of miR-19a markedly suppressed cell proliferation, migration and induced apoptosis of vascular smooth muscle cells. Silencing of miR-19a significantly decreased the levels of inflammatory cytokines and matrix metalloproteinase. Dual-luciferase reporter gene assay results showed that miR-19a decreased the expression of CDKN2B by binding to its 3’-untranslated region (UTR). Upregulation of CDKN2B attenuated the inhibitory effect of miR-19a-elevated on the proliferation and migration of vascular smooth muscle cells and inflammatory response of THP-1 cells. Conclusion: Knockdown of miR-19a could alleviate the development of AAA by inhibiting the proliferation and migration of smooth muscle cells and down regulating inflammatory cell infiltration, which its mechanism is to target up-regulation of CDKN2B expression. 2020 Vol. 50 (8): 609-615，622 [Abstract] ( 798 ) HTML (1 KB)  PDF (2568 KB)  ( 760 ) 616 Efficacy and safety of CD19-CART in the treatment of relapsed refractory non-Hodgkin’s B-cell lymphoma HE Mingxia, LAI Jin, WENG Yiqin, MA Yongyong, LIN Fengyang, XING Chongyun, JIANG Songfu DOI: 10.3969/j.issn.2095-9400.2020.08.003 Objective: To investigate the therapeutic efficacy and safety of CD19 chimeric antigen receptor T cells (CART) for the relapsed/refractory B-cell non-Hodgkin’s lymphoma (B-NHL). Methods: After close follow-up, the short-term and long-term response and adverse effect (AEs) of 10 patients with B-NHL who received CD19-CART therapy from April 2017 to October 2018 were evaluated. Results: All patients were with relapsed/refractory lymphoma. After infusion of CD19-CART cells in 10 patients, the total complete response (CR) rate was 30%, the partial response (PR) rate was 40%, and the overall response rate was 70%. In vivo proliferation of CD19-CART cells was detected in peripheral blood of all patients, which decreased rapidly after about 3 months. The median follow-up was 9 (1-24) months. The median overall survival (OS) of all patients was (10.5±4.0) months, and the median progression-free survival (PFS) was (4.6±3.9) months. A total of 8 patients (8/10) had cytokine release syndrome (CRS) after infusion of CART cells, of which five were grade 1 CRS, two were grade 2 CRS and one was grade 4 CRS accompanying with grade 4 neurotoxicity. Two patients used Tocilizumab (IL-6 receptor monoclonal antibody), and one patient was treated combined with glucocorticoids. Except for one patient suffering from grade 4 CRS who died of grade 4 neurotoxicity on the 18th day after infusion, the other patients with CRS were effectively controlled. Conclusion: Since CART therapy for relapsed/refractory CD19+ B cell lymphoma has achieved certain effect in some ways and the AEs were controllable, it could be used as a salvage treatment for relapsed/refractory patients. Timely monitoring and management of AEs during the infusion period might help prevent the uncontrollable outcomes. 2020 Vol. 50 (8): 616-622 [Abstract] ( 991 ) HTML (1 KB)  PDF (1440 KB)  ( 1561 ) 623 Preparation of acryloyloxyethyl trimethyl ammonium chloride conductive hydrogel and its performance detection XUE Yujie, XU Lifei, ZHANG Qilu, WANG Jiaqian, XU Ke, LIN Luyang DOI: 10.3969/j.issn.2095-9400.2020.08.004 Objective: To screen out the optimal formulation of hydrogels with excellent mechanical properties and electrical conductivity. Methods: A composite conductive hydrogel was prepared by using acryloyloxyethyl trimethyl ammonium chloride (DAC) as the main matrix, N, N-methylenebisacrylamide (BisAAm) as the chemical cross-linking agent, and 1-hydroxycyclohexyl benzophenone as the catalyst, added with glycerol and hydroxyethyl cellulose (HEC). Its conductivity, viscosity, tensile properties and compression properties were measured. Results: After using DAC as the matrix and adding BisAAm, glycerin, and HEC in proportion, the gel had no odor or residue compared with 3M conductive hydrogel, with good long-term stability, without color change and bacterial growth. At the same time, after adding glycerin and HEC by proper proportions, its conductivity, viscosity, tensile properties and compression properties were better than traditional 3M conductive gels. It was found through mouse skin contact experiments that the hydrogels had good biological safety. Conclusion: The hydrogel DAC content is at its best when its content is 60% and the proportion of BisAAm, glycerol and HEC is 5%, 10% and 0.015% respectively. 2020 Vol. 50 (8): 623-627 [Abstract] ( 767 ) HTML (1 KB)  PDF (1668 KB)  ( 877 ) 628 Clinical significance of lncRNA RP11-86H7.1 in the diagnosis of Kawasaki disease PAN Lulu, LU Kongchang, CHU Maoping, ZENG Jingjing, RONG Xing DOI: 10.3969/j.issn.2095-9400.2020.08.005 Objective: To investigate the expression of lncRNA RP11-86H7.1 in serum of Kawasaki disease (KD) patients and its correlation with clinicopathological characteristics and prognosis. Methods: The circulating lncRNA specifically related to KD were screened and divided into four groups: children before KD treatment, children after KD treatment, children with common fever and healthy children group. The results of lncRNA microarray showed that excluding the influence of fever, the expression of lncRNA in plasma of kawasaki patients was higher than that in normal children and during recovery period. The relative expression of serum lncRNA RP11-86H7.1 in the normal children group and the group before KD treatment was detected by real-time fluorescence quantitative PCR. The correlation between the expression of serum lncRNA RP11-86H7.1 and the clinicopathological characteristics of kawasaki disease was analyzed; ROC curve was drawn to analyze the diagnostic effect of serum lncRNA RP11-86H7.1 expression level on kawasaki disease. Results: The relative expression of serum lncRNA RP11-86 H7.1 in the children’s group before KD treatment was higher than that in the control group (P<0.05). There was no significant difference in age and sex ratio between the low-expression group and the low-expression group (P>0.05). Real-time PCR showed that the expression level of lncrnarp11-86H7.1 in serum of KD patients in acute stage was significantly higher than that in KD recovery stage, normal control group and fever control group. The differences were statistically significant (P<0.05). Conclusion: Serum lncRNA RP11-86H7.1 was up-regulated in patients with KD, and it can be used as a potential biomarker for early diagnosis and prognosis evaluation of KD. 2020 Vol. 50 (8): 628-631，636 [Abstract] ( 656 ) HTML (1 KB)  PDF (1422 KB)  ( 920 ) 632 Clinical characteristics of Holmes tremor and its diagnosis and treatment LYU Xinhuang, HONG Qian, TONG Qiuling, LI Jing, ZHANG Yang, CHEN Weian DOI: 10.3969/j.issn.2095-9400.2020.08.006 Objective: To explore the clinical characteristics, etiology, imaging manifestations and treatment of Holmes tremor (HT). Methods: A retrospective analysis of the medical records and videos of 6 HT patients in the First Affiliated Hospital of Wenzhou Medical University from September 2015 to June 2018 was made. Results: A total of 2 females and 4 males were included, aged ranging 14-67 years at the moment of CNS insult. Etiology showed hemorrhage in 3 cases, infarction in 1 case, traumatic brain injury in 1 case, and optic neuromyelitis spectrum disease in 1 case. The median latency from lesion to tremor onset was 2 months (ranging from 14 days to 8 months). Symptoms/signs associated with HT included hemiplegia in 4 cases, ataxia in 3 cases, hypoesthesia in 3 cases, dystonia in 2 cases, cranial nerve involvement, dysarsaras and palatal clonus in 1 case. MRI showed lesions in midbrain 5 cases, thalamus 4 cases, and pontine in 1 case. In the short term, levodopa treatment significantly relieved 1 case and moderately relieved 2 cases: in 3 cases with combined treatment of clonazepam, 2 cases were significantly alleviated, but ineffective in 1 case. Three-year follow-up of 3 cases showed decline in effect. Conclusion: The most common causes of HT in our series were vascular lesions. The most common lesion topography was mesencephalic, thalamic, or both. Levodopa and clonazepam have a certain therapeutic effect in the short term, but the long-term therapeutic effect is decreased. 2020 Vol. 50 (8): 632-636 [Abstract] ( 910 ) HTML (1 KB)  PDF (1421 KB)  ( 853 ) 637 The association between functional liver volume and liver function evaluated by quantitative analysis of gadoxetic acid-enhanced magnetic resonance imaging CAI Jiale, WANG Zhaohong, CHEN Hui, TONG Hongfei DOI: 10.3969/j.issn.2095-9400.2020.08.007 Objective: To compare correlations between FLV and Child-Pugh classification of liver function. Methods: All 25 patients with liver cirrhosis performed enhanced CT and Gd-EOB-DTPA-enhanced MRI were recruited in this study. The liver function of all cases was evaluated by Child-Pugh classification. The contrast enhancement ratio (CER) was analyzed by SIlh-SIlu/SIlu, and the volume of each liver segment measured by MI-3DVS. Then correlations between anatomical liver volume, functional liver volume and Child-Pugh classification of liver function analyzed. Results: Analyses showed that Child-Pugh classification of liver function was negatively correlated to the ALV (r=-0.792, P<0.001), with determination coefficient of curve fitting (R2=0.63); Child-Pugh classification of liver function was also negatively correlated with FLV (r=-0.911, P<0.001), determination coefficient of curve fitting (R2=0.80). Compared between the two analyzing methods of liver volume, FLV had more significant negative correlation with Child-Pugh classification of liver function. Conclusion: The FLV calculated by CER of Gd-EOB-DTPA-enhanced MRI combined with ALV from MI-3DVS could better reflect the liver function than ALV. 2020 Vol. 50 (8): 637-641 [Abstract] ( 625 ) HTML (1 KB)  PDF (1869 KB)  ( 989 ) 642 The significance of serum retinol binding protein in patients with nonalcoholic fatty liver disease PAN Jie, GAO Min, JI Guofei DOI: 10.3969/j.issn.2095-9400.2020.08.008 Objective: To explore the possible association between serum retinol binding protein (RBP) and nonalcoholic fatty liver disease (NAFLD). Methods: From October 2017 to August 2018 in Huzhou Central Hospital, 167 patients with NAFLD diagnosed by abdominal ultrasonography and 141 healthy controls were enrolled in the study and their fasting serum RBP levels and other clinical and laboratory parameters were measured. Results: NAFLD patients had significantly higher serum RBP levels than controls (P<0.05). Serum RBP level was significantly and positively correlated with parameters of metabolic syndrome (BMI, fasting blood glucose), inflammation indexes (sialic acid, hypersensitive C-reactive protein), and markers of hepatocellular damage (alanine aminotransferase, alanine aminotransferase) (P<0.05). Subgroup (obesity, triglycerides, fasting blood glucose) analysis showed that serum RBP level was significantly increased in NAFLD subjects with individual components of obesity, hypertriglyceridaemia, and hyperglycaemia. Multivariate logistic regression analysis showed that the increase of RBP level (OR=2.580, 95%CI=1.718-3.356, P<0.001) was an independent risk factor for the occurrence and development of NAFLD. Conclusion: Serum RBP level is closely related to NAFLD. 2020 Vol. 50 (8): 642-646 [Abstract] ( 615 ) HTML (1 KB)  PDF (1340 KB)  ( 845 ) 647 Fat suppression T2WI with radiomics analysis in differential diagnosis of leiomyoma and adenomyoma CHEN Cheng, YE Miaomiao, CHEN Bo, ZHU Xueqiong DOI: 10.3969/j.issn.2095-9400.2020.08.009 Objective: To identify the value of preoperative fat suppression T2WI of pelvic magnetic resonance imaging (MRI) for differential diagnosis of leiomyoma and adenomyoma with radiomics analysis. Methods: This retrospective study included 120 cases of patients with leiomyoma or adenomyoma pathologically confirmed by surgery in the Second Affiliated Hospital of Wenzhou Medical University between April 2015 and September 2019. All patients underwent conventional MRI plain scan of pelvic before surgery. According to the ratio of 7:3, all the patients were randomly divided into training set (n=84) and verification set (n=36). Region of interest (ROI) was manually delineated on fat suppression T2WI, and subsequently the high-throughput data collection, radiomics features extraction and dimensionality reduction were performed. Multivariate logistic regression was used to build up the prediction model of the differential diagnosis of leiomyoma and adenomyoma. Moreover, the sensitivity, specificity, accuracy and the area under the receiver-operating characteristic curve (AUC) were used for evaluating the predictive performance of the model. Results: A total of 396 radiomics features were extracted and 6 radiomics features were included in the prediction model of the differential diagnosis of leiomyoma and adenomyoma. In the prediction model, AUC in the training set was 0.861, with sensitivity being 84.1% (37/44), specificity 82.5% (33/40) and accuracy 83.3% (70/84), while AUC in the verification set was 0.913, with sensitivity being 84.2% (16/19), specificity 94.1% (16/17) and accuracy 88.9% (32/36). Conclusion: The radiomics prediction model based on fat suppression T2WI can well differentiate the leiomyoma from adenomyoma, which potentially provides a favorable and non-invasive method for clinical differential diagnosis. 2020 Vol. 50 (8): 647-651 [Abstract] ( 788 ) HTML (1 KB)  PDF (1457 KB)  ( 968 ) 652 Analysis of risk factors and prognosis of anterior communicating artery aneurysm complicated with intraventricular hemorrhage in the elderly ZHOU Jiafeng, CHEN Yongchun, LIN Boli, CHEN Lifang, XIONG Ye, YANG Yunjun DOI: 10.3969/j.issn.2095-9400.2020.08.010 Objective: To investigate the risk factors and prognosis of intracerebral and/or intraventricular hematoma (ICH and/or IVH) after rupture of AComA (anterior communicating artery) aneurysms in elderly and young patients. Methods: From December 2007 to January 2016, a total of 285 consecutive patients suffering from ruptured AComA aneurysms with ICH and/or IVH that admitted to the department of neurosurgery in the First Affiliated Hospital of Wenzhou Medical University, had been enrolled into this retrospective study. Among them, 172 patients underwent clipping, 244 patients underwent Coiling, and 64 patients underwent medical treatment. Patients were divided into two groups based on age:patients of age≤60 years (n=168) and patients of age>60 years (n=117). All patients had been examined by CT angiography (CTA) before surgery and the morphological parameters of the aneurysm had been measured. Single and multiple variate logistic regression methods were used to analyze the risk factors and prognosis of ruptured AComA aneurysms with ICH and/or IVH in elderly and young. Results: The univariate analysis revealed that the two groups had statistically significant differences in the gender, hypertension history, smoking history, vessel angle, flow angle, treatment and GOS score (all P<0.05). The multivariate analysis revealed that hypertension history (OR=1.858, 95%CI=1.083-3.188, P=0.025), flow angle (OR=1.016, 95%CI=1.004-1.028, P=0.010) and treatment (coiling vs. medical treatment OR=0.209, 95%CI=0.088-0.497, P<0.001; clipping vs. medical treatment OR=0.233, 95%CI=0.101-0.541, P=0.001) were closely associated with elderly patients of ruptured AComA aneurysms with ICH and/or IVH. The prognosis analysis of different treatment methods in the elderly group had showed that the prognosis of patients receiving surgical treatment was relatively good (coiling vs. medical treatment OR=0.134, 95%CI=0.049-0.362, P<0.001; clipping vs. medical treatment OR=0.308, 95%CI=0.116-0.820, P=0.018). Conclusion: Hypertension and bigger flow angle are the risk factors of ICH and/or IVH induced by ruptured AComA aneurysms, which is more obvious in the elderly. The operation rate of the elderly group is significantly lower than that of the young group, but the elderly patients after surgical treatment still has a better prognosis. 2020 Vol. 50 (8): 652-656，661 [Abstract] ( 732 ) HTML (1 KB)  PDF (1342 KB)  ( 637 ) 657 Risk factors of abdominal Henoch-Schönlein purpura in children WENG Shengliang, ZHANG Youla DOI: 10.3969/j.issn.2095-9400.2020.08.011 Objective: To investigate the risk factors of children with abdominal Henoch-Schönlein purpura (HSP) and to provide the predictors for early clinical diagnosis and treatment. Methods: Ninety-eight children with HSP from the Fifth Hospital of Jinhua during January 2011 and December 2018 were collected, which included 50 abdominal HSP and 48 skin-type HSP. The clinical characteristics and laboratory indicators of those children between two groups were compared. The risk factors of HSP in children were analyzed by logistic regression analysis. Results: The incidence of upper respiratory tract infection in children with abdominal HSP was significantly higher than that in children with skin HSP (P<0.05). The laboratory indicators of white blood cell count, neutrophil count, C-reactive protein, D-dimer in children with abdominal HSP were significantly higher than those of skin-type HSP (P<0.05). There were no significant differences between the two groups (P>0.05) in other indicators such as gender, age, season of onset, hospitalization days, hemoglobin, platelet count, serum potassium, alanine aminotransferase, aspartate aminotransferase, serum albumin, ESR, complement C3, C4, IgA, prothrombin time and activated partial prothrombin kinase time were not significantly different between the two groups (P>0.05). Logistic regression analysis showed that upper respiratory tract infection (OR=3.54, 95%CI=1.29-9.75, P=0.014) and high D-dimer (OR=4.23, 95%CI=1.28-13.96, P=0.018) were independent risk factors for HSP in children. Conclusion: Children of HSP with risk factors such as upper respiratory tract infection and high D-dimer should be intervened early and treated properly to prevent complications. 2020 Vol. 50 (8): 657-661 [Abstract] ( 678 ) HTML (1 KB)  PDF (1361 KB)  ( 743 ) 662 Expression of a-1, 2 fucosyltransferase II in lung squamous cell carcinoma and its effect on proliferation of lung squamous cell carcinoma cells WANG Weijie, FAN Sairong, ZHANG Guanghui, SUN Haiting, SHI Guofu, MA Xuan, ZHU Chengchu DOI: 10.3969/j.issn.2095-9400.2020.08.012 Objective: To investigate the expression of a-1, 2 fucosyltransferase II (FUT2) in human lung squamous cell carcinoma (LUSC) and FUT2 effect on the cell proliferation of LUSC. Methods: UALCAN database was used to analyze the expression and clinical significance of FUT2 in lung squamous carcinoma; the shRNA plasmid of FUT2 was transferred into LUSC’s cell line (H226) by transient transfer technique to construct the knockdown expression of FUT2; CCK-8, colony formation and Western blot were used to investigate the effect of FUT2 on cell proliferation of LUSC-cell growth, monoclonal cell mass formation and the expression of proliferator PCNA protein. Results: The expression of FUT2 in LUSC’s tumor tissue was higher than that in normal control tissue, especially in the early and middle stages (P<0.05). But the expression of FUT2 had no relations with LUSC patients’ age and sex. In addition, the lower expression of FUT2 inhibited the growth and formation of monoclonal cell clusters as well as repressed the expression of PCNA protein in LUSC’s cell line, which all has statistical significance (P<0.05). Conclusion: FUT2 is highly expressed in LUSC and could promote the cell proliferation of LUSC, which might become a diagnostic index of LUSC. 2020 Vol. 50 (8): 662-665 [Abstract] ( 662 ) HTML (1 KB)  PDF (1449 KB)  ( 725 ) 666 Relationship between serum prolactin and cognitive function in patients with first episode schizophrenia LYU Wei, ZHU Cheng, ZHENG Ke, LIU Jiahong, LIU Linjing DOI: 10.3969/j.issn.2095-9400.2020.08.013 Objective: To compare the levels of serum prolactin and cognitive function between first episode schizophrenic patients and healthy controls, and further analyze the relationship between cognitive function and homocysteine in schizophrenic patients. Methods: Eighty first episode schizophrenic patients and eighty normal persons were selected as case group and control group from January to December 2018 in the Affiliated Kangning Hospital of Wenzhou Medical University. After entering the group, the plasma level of prolactin and the score of Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) were measured in schizophrenic patients and healthy controls. The collected data were analyzed by correlation analysis and stepwise regression analysis. Results: The serum level of prolactin in schizophrenic patients was significantly higher than that in healthy controls, while the total score of RBANS and its factor scores in schizophrenic patients were significantly lower than that in healthy controls (P<0.05). The serum prolactin level was negatively correlated with the total score of RBANS, the factor score of visual span and speech function (r=-0.423, -0.271, -0.352, P<0.05), but not with the factor score of attention, immediate memory and delayed memory (P>0.05). The serum prolactin level entered the regression equation with RBANS total score, visual span and speech function factors score as dependent variables, that could explain 25.70%, 14.70% and 19.60% of the changes of RBANS total score, visual span and speech function factors score respectively (P<0.05). Conclusion: Patients with schizophrenia have elevated serum prolactin levels and cognitive impairment. The abnormity of serum prolactin in patients with schizophrenia is associated with cognitive impairment. 2020 Vol. 50 (8): 666-669 [Abstract] ( 834 ) HTML (1 KB)  PDF (1378 KB)  ( 1151 ) 670 Cone beam CT study of root anatomy and root canal morphology in mandibular premolars WANG Min, DU Yu, LIU Guo, PAN Yihuai. DOI: 10.3969/j.issn.2095-9400.2020.08.014 Objective: To investigate the root anatomy and root canal morphology of mandibular premolars using Cone Beam CT and to determine their degree of bilateral symmetry. Methods: CBCT images of 413 patients were included (794 mandibular first premolars and 770 mandibular second premolars). The images were analyzed using NNTViewer software to check for the number of roots, the location of radicular grooves, the number of root canals, the canal configurations, and the bilateral symmetry of root canals. Results: The majority of the mandibular first premolars were single-rooted, only 13 teeth were double-rooted. The canal configuration of mandibular first premolars was type I: 87.66%, type II: 1.76%, type III: 2.52%, type IV: 2.14%, type V: 5.92%, and C-shaped canal: 12.21% (according to the cross-section), respectively. 118 mandibular first premolars had radicular grooves, which were all located on the mesio-lingual side. The bilateral symmetry rate of canal configuration was 93.70%. All the mandibular second premolars were single-rooted. 99.48% of root canals were type I and 0.52% were type V. Four mandibular second premolars had radicular grooves on the mesiolingual side. 99.48% of mandibular second premolars exhibited bilateral canal symmetry. Conclusion: The root anatomy and root canal morphology of mandibular premolars are complex but symmetrical. CBCT aids in characterizing the the configurations of the canal system, which can improve the success rate of root canal treatment. 2020 Vol. 50 (8): 670-673 [Abstract] ( 707 ) HTML (1 KB)  PDF (1374 KB)  ( 835 ) 674 The effect of delayed ICU transfer on the incidence of ventilator-associated pneumonia in patients with endotracheal intubation HUANG Ying, SU Chunxia DOI: 10.3969/j.issn.2095-9400.2020.08.015 Objective: To investigate the effect of delayed ICU transfer on the incidence of ventilator-associated pneumonia (VAP) in patients with endotracheal intubation. Methods: A total of 348 patients admitted to the ICU of Shanghai Pulmonary Hospital of Tongji University from June 2016 to June 2018 were enrolled in this study, who were divided into delayed group (n=87) and non-delayed group (n=261). The χ2 test was used to compare the incidence of VAP in the two groups. Logistic regression model was performed to study the effect of delayed ICU transfer on VAP. Results: The incidence of VAP in the delayed ICU group was significantly higher than that in the non-delayed group (χ2=21.426, P<0.001). Except for the delayed transfer to ICU, among the influencing factors of VAP, there were statistically significant differences between the groups in age, antibiotic history, enteral nutrition, and coma (P<0.05). After deducting the effect of gastrointestinal nutrition and conscious state, delayed ICU transfer was an independent risk factor for VAP compared with non-delayed ICU (OR=2.644, 95%CI=1.494-4.677, P=0.001). In addition, gastrointestinal nutrition (OR=3.419, 95%CI=1.980-5.905, P<0.001) and coma (OR=2.681, 95%CI=1.542-4.661, P<0.001) were also independent risk factors for VAP. Conclusion: Delayed ICU transfer could result in an increased incidence of VAP. Therefore, critically ill patients with endotracheal intubation should be provided with standardized VAP precautions as soon as possible before ICU transfer so as to reduce the occurrence of VAP. 2020 Vol. 50 (8): 674-676 [Abstract] ( 710 ) HTML (1 KB)  PDF (1327 KB)  ( 716 ) 677 The effect of narrative nursing intervention on job burnout of nurses WU Binbin, LEI Xuezhen, LUO Hong, FENG Yaping, SHAO Ganqin DOI: 10.3969/j.issn.2095-9400.2020.08.016 Objective: To explore the impact of narrative care on nurses in clinical care work. Methods: From January to June 2018, a group of 88 nurses from each ward of the Affiliated Hospital of Hangzhou Normal University underwent a six-month group narrative nursing intervention. Before and after the intervention, the Mars burnout scale was used to evaluate the nurses, and the differences in nurse burnout before and after the intervention were compared. Results: After the intervention, the scores of emotional exhaustion, depersonalization, low sense of achievement and positive proportion of nurses all decreased compared with those before the intervention (P<0.05). The proportion of mild, moderate and severe job burnout among nurses decreased after the intervention compared with that before the intervention (χ2=3.769, P=0.049). Conclusion: The application of narrative care in clinical nursing work can effectively reduce the occurrence of nurse burnout. 2020 Vol. 50 (8): 677-679 [Abstract] ( 723 ) HTML (1 KB)  PDF (1326 KB)  ( 833 )

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