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2020 Vol. 50, No. 7 Published: 2020-07-25   517 The role of RSK2 in promoting skin wound healing by regulating the proliferation and migration of human skin fibroblasts WANG Jia’nan, ZHU Zhongxin, CONG Weitao DOI: 10.3969/j.issn.2095-9400.2020.07.001 Objective: To investigate the effect of RSK2 on skin wound healing and its mechanism. Methods: Western blot and immunofluorescence staining were used to detect the expression of RSK2 under wound conditions. Scratch test, CCK-8 detection and Western blot were used to investigate the role of RSK2 inhibitor Bix 02565 on human primary dermal fibroblasts (HDF) at the cellular level. In vivo, HE staining, Masson staining and immunofluorescence staining were used to detect the effect of RSK2 inhibitor on skin wound repair. Results: Western blot and immunofluorescence staining showed that the skin trauma modal did not affect the total protein expression of RSK2 in fibroblasts, while the use of Bix 02565 to inhibit the activity of RSK2 in vitro could significantly inhibit the proliferation and migration of fibroblasts, and inhibit the expression of G1/S-specific cyclin-D1 and Fibronectin. In vivo, the inhibitor restrained proliferating cell nuclear antigen (PCNA) and collagen fibers production of fibroblasts, inhibited platelet-endothelial cell adhesion molecule (CD31), blocked vessels regeneration and delayed the repair rate of wound healing in mice. Conclusion: RSK2 facilitates skin wound healing mainly by promoting the proliferation and migration of fibroblasts and via angiogenesis. 2020 Vol. 50 (7): 517-524 [Abstract] ( 932 ) HTML (1 KB)  PDF (2883 KB)  ( 1232 ) 525 The effect of linc01635 on apoptosis of vascular endothelial cells in Kawasaki disease CHEN Ruiyao1, RONG Xing, WU Tingting, JIA Chang, WU Rongzhou, CHU Maoping, ZHANG Chunxiang DOI: 10.3969/j.issn.2095-9400.2020.07.002 Objective: To investigate the effect of linc01635 on apoptosis of vascular endothelial cells in Kawasaki disease (KD). Methods: Bioinformatics prediction and RNA FISH was employed to detect Subcellular localization of linc01635. Human umbilical vein endothelial cells (HUVECs) were cultured using KD plasma and healthy control plasma, qRT-PCR was used to detect the relative expression of linc01635 in HUVECs. Linc01635 expression in HUVECs was knocked-down by linc01635 siRNA and up-regulated by lentivirusor. Flow cytometry was used to analyze the apoptosis of HUVECs. Apoptosis was induced by KD plasma and the effect of linc01635 on KD-induced vascular endothelial cell injury was detected. Results: Linc01635 was distributed in both nuclei and cytoplasm of HUVECs. Compared with the healthy control group, KD plasma-treated HUVECs exhibited reduced expression of linc01635 (P<0.01). Overexpression of linc01635 inhibited HUVECs apoptosis (P<0.01), while knockdown of linc01635 increased HUVECs apoptosis (P<0.01). KD plasma could promote HUVECs apoptosis, and overexpression of linc01635 could inhibit KD plasma-induced apoptosis (P<0.01). Conclusion: KD plasma treatment can reduce the expression of linc01635 in vascular endothelial cells, which decreased linc01635 via KD plasma stimulation can induce vascular endothelial cells apoptosis. 2020 Vol. 50 (7): 525-529 [Abstract] ( 750 ) HTML (1 KB)  PDF (1652 KB)  ( 943 ) 530 The effect of long chain fat emulsion on the myocardial ischemia reperfusion injury in rats DONG Jiaojiao, XIA Fangfang, JIN Zhousheng, ZHANG Yujian, LIU Le DOI: 10.3969/j.issn.2095-9400.2020.07.003 Objective: To investigate the protective effect of long chain fat emulsion on myocardial ischemia/reperfusion (I/R) injury in rats. Methods: Twenty-one adult SD rats were randomly divided into three groups (n=7): sham operation group (Sham group), no drug treatment; model group (Model group), normal saline 2 mg·kg-1·d-1 injection for 5 days; long chain fat emulsion group (LE group), intralipid 2 mg·kg-1·d-1 injection for 5 days. Twenty-four hours after the last administration, the model of myocardial ischemia reperfusion (I/R) injury in rats was established by ligating the left anterior descending coronary. Hemodynamics was continuously monitored. The heart was taken for infarction area analysis, and the blood was collected for lactate dehydrogenase (LDH) detection. Results: Compared with the Model group, infarct size divided by area at risk (IR/AAR) and infarct size divided by left ventricle (IR/LV) were significantly reduced in LE group (all P<0.05), and LDH release was markedly lower than that in the Model group (P<0.05). The mean arterial pressure (MAP) and rate-pressure product (RPP) of rats in Model group were statistically lower than Sham group in ischemic period and reperfusion period (all P<0.05), while there was no significant difference between LE group and Sham group (P>0.05). Conclusion: Long chain fat emulsion pretreatment can reduce infarct area after myocardial I/R injury in rats, reduce LDH release and alleviate hemodynamic changes caused by I/R injury. 2020 Vol. 50 (7): 530-534 [Abstract] ( 803 ) HTML (1 KB)  PDF (1601 KB)  ( 830 ) 535 The protective effect of total flavonoids of cortex mori on airway inflammation in asthmatic mice GE Shuyu, LI Lan, SUN Pingping1 DOI: 10.3969/j.issn.2095-9400.2020.07.004 Objective: To investigate the protective effect of total flavonoid of cortex mori (TFCM) on airway inflammation in asthmatic mice and its mechanism. Methods: C57BL/6 mice were randomly divided into normal group, model group, groups of low, medium and high dose TFCM and dexamethasone positive control group. Abnormal groups were sensitized and induced with ovalbumin (OVA). TFCM (50, 100, 200 mg/kg) was administered to each dose group while the positive control group was given dexamethasone (0.5 mg/kg). The normal group and the model group were given the same amount of saline. The mice were placed in an animal lung function testing system to measure lung resistance and lung compliance. Bronchoalveolar lavage fluid (BALF) and blood samples of the mice were collected to determine IL-4, IL-5, IL-13, Eotaxin, IgE, mucin MUC5AC, MUC5B levels and total cell count, white blood cell differential counts. Airway inflammation was observed by HE and AB-PAS staining in lung tissues. Results: Compared with the model group, lung resistance was significantly increased in all dose groups of TFCM, and lung compliance was significantly decreased (P<0.05). The number of total cells, neutrophils, eosinophils and macrophages in BALF in the middle and high dose groups of TFCM were significantly lower than those in the model group (P<0.05). The levels of IL-4 and IgE in BALF in the middle and high dose groups were significantly lower than those in the model group (P<0.05). The IL-5 level in the high dose group was significantly lower than that in the model group (P<0.05). The levels of IL-13, Eotaxin, MUC5AC and MUC5B in each dose groups of TFCM were significantly lower than those in the model group (P<0.05). The HE and AB-PAS staining results showed that the airway inflammation of the lung tissue of the TFCM dose group was significantly reduced compared with the model group. Conclusion: TFCM can reduce the airway inflammation and have a significant effect on asthma in mice. 2020 Vol. 50 (7): 535-540 [Abstract] ( 727 ) HTML (1 KB)  PDF (1615 KB)  ( 871 ) 541 Detection and analysis of 3 Leber’s hereditary optic neuropathy families carrying tRNALeu(CUN) 12308A>G mutation WANG Xuan, LIANG Min, GUAN Minxin DOI: 10.3969/j.issn.2095-9400.2020.07.005 Objective: To explore whether the inferred tRNALeu(CUN) 12308A>G mutation affects the phenotypic expression of Leber’s hereditary optic neuropathy (LHON). Methods: Screening of 295 unrelated Han LHON patients and 316 normal controls were performed for mitochondrial mutation genes, and three screened families carrying m.12308A>G mutation and having typical LHON clinical manifestations were analyzed for mitochondrial complete sequence analysis, haplotype analysis and mitochondrial copy number measurement. Results: None of the three pedigrees carried the major primary locus, but a m.12308A>G homogeneous mutation occurred at 44 positions, which was highly conserved on tRNALeu(CUN). The haplotypes of the three probands belonged to East Asian haplotype H2, but the age of onset and the degree of visual impairment were different and no 12308A>G mutation was detected in normal controls. It was found that the mitochondrial copy number of the mutant cells was significantly reduced in the copy number measuremen. Conclusion: The m.12308A>G mutation may be a mutation site associated with LHON. However, the low penetrance of the mutant family indicates that the mutation itself is not sufficient to cause the phenotypic expression of LHON, and that other modification factors (nuclear gene modification, mitochondrial haplotype and environmental factors) have played a role in the pathogenesis of LHON. 2020 Vol. 50 (7): 541-546，552 [Abstract] ( 739 ) HTML (1 KB)  PDF (1958 KB)  ( 982 ) 547 Differential value of thin-slice CT features of lung mixed ground glass nodules between minimally invasive adenocarcinomas and invasive adenocarcinoma YE Shengli, XU Shunliang, ZHENG Xiaoping, HU Wenchao, JIANG Jiakang, YANG Li DOI: 10.3969/j.issn.2095-9400.2020.07.006 Objective: To explore the differential value between lung minimally invasive adenocarcinoma (MIA) and invasive adenocarcinoma (IAC) manifesting as mixed ground-glass nodule (MGGN) by analyzing the imaging features of thin section computed tomography (TSCT), so as to help thoracic surgeons to choose a reasonable surgical method for patients. Methods: There were 200 patients with 216 pathologically diagnosed MGGN (107 MIA and 109 IAC) from the First Affiliated Hospital of Zhejiang University School of Medicine included in this study. By comparing the imaging features of the two groups, the invasive risk factors were analyzed by multivariate logistic regression, and the diagnostic efficiency of the imaging features was analyzed by ROC curve. Results: There were statistical differences between MIA group and IAC group in clear border, lobulation sign, nodule diameter, the diameter of the solid component, the CT values of nodule and ground-glass components (P<0.05). The results of multivariate logistic regression analysis showed clear border (OR=0.063, 95%CI=0.008-0.507, P=0.009) and lobulation sign (OR=0.383, 95%CI=0.147-0.993, P=0.048) were the risk factors for their invasion. However, the greater the CT values of nodule (OR=1.007, 95%CI=1.001-1.014, P= 0.029), the CT values of ground-glass components (OR=1.007, 95%CI=1.001-1.013, P=0.030), the diameter of the solid component (OR=337.004, 95%CI=17.431-6 515.57, P<0.001) and the diameter of the nodule (OR= 3.528, 95%CI=1.146-10.862, P=0.028), the more likely it was for MGGN to be pathologically confirmed as IAC. The corresponding diagnostic sensitivity, specificity, areas under the ROC curve (AUC) of clear border were 9.35%, 97.85% and 0.536, respectively; lobulation sign was 58.88%, 59.63% and 0.593, respectively. The corresponding diagnostic cut-off value, sensitivity, specificity, AUC of CT values of nodule were -408 Hu, 77.57%, 84.40% and 0.881%, respectively; CT values of ground-glass components were: -496 Hu, 86.92%, 71.56% and 0.835, respectively; the solid component was 0.4 cm, 89.72%, 99.08% and 0.954% respectively in diameter; the nodule was 1.1 cm, 57.94%, 82.57% and 0.756 respectively in diameter. Conclusion: The MIA were significantly associated with less clear border, less lobulation sign, a smaller diameter of the solid component (≤4 mm)and nodule diameter (≤11 mm), and lower CT values of nodule (≤-408 Hu) and ground-glass components (≤-496 Hu) in the MGGN. Thin-slice CT imaging features can be helpful for the differential diagnosis of MIA and IAC manifesting as MGGN. 2020 Vol. 50 (7): 547-552 [Abstract] ( 1142 ) HTML (1 KB)  PDF (1563 KB)  ( 1637 ) 553 The regulatory role of RanBPM in renal tubular sodium chloride cotransporter and its effect on ERK1/2 signaling pathway ZHANG Yiqian, ZHUANG Zhizhi, SHEN Meng, ZHUANG Jieqiu, CAI Hui DOI: 10.3969/j.issn.2095-9400.2020.07.007 Objective: To observe the effect of Ran binding protein M (RanBPM) on the expression of NCC protein in renal tubules and the change of ERK1/2 signaling pathway protein. Methods: Immunocoprecipitation was used to detect whether there was direct interaction between RanBPM and WNK4 protein. The effect of transfection of RanBPM plasmid on ERK1/2 phosphorylation induced by EGF was observed. RanBPM gene was inhibited by overexpression of RanBPM protein or siRNA. The content of endogenous NCC protein and the level of ERK1/2 phosphorylation were detected by Western blot. The effect of RanBPM on WNK4 regulation of NCC by ERK1/2 phosphorylation was explored before and after transfection. Results: Immunocoprecipitation confirmed that there was a direct interaction between WNK4 and RanBPM. RanBPM could inhibit EGF induced ERK1/2 phosphorylation. Overexpression of RanBPM reduced ERK1/2 phosphorylation (1.000±0.074 vs. 0.275± 0.041, P<0.01) and increased NCC protein expression (1.000±0.115 vs. 1.470±0.105, P<0.01). After siRNA silenced RanBPM gene, ERK1/2 phosphorylation increased (1.000±0.194 vs. 2.301±0.220, P<0.01), resulting in decreased NCC protein expression (1.000±0.223 vs. 0.556±0.132, P<0.01). When RanBPM was transfected, WNK4 inhibited NCC protein expression and no longer affected ERK1/2 phosphorylation. Conclusion: RanBPM interacted with WNK4 affects the regulation of ERK1/2 signaling pathway in NCC protein expression. 2020 Vol. 50 (7): 553-556，562 [Abstract] ( 672 ) HTML (1 KB)  PDF (1671 KB)  ( 836 ) 557 The effects of L-mimosine on apoptosis and DNA damage-related protein expression in human pharyngeal squamous cell carcinoma FaDu cells XIANG Yinzhou, LI Xuejun, ZOU Yuhua, ZHOU Huajun DOI: 10.3969/j.issn.2095-9400.2020.07.008 Objective: To explore the effect of L-mimosine on FaDu cells apoptosis and DNA damage related protein expression in human pharyngeal squamous cell carcinoma. Methods: The growth of FaDu cells after treating with L-mimosine at different time was observed by an inverted microscope. Flow cytometry was used to detect the apoptosis effects after different concentrations of L-mimosine and ammonium ferric citrate treating FaDu cells. Cell growth proliferation activity was examined by CCK-8 method after different concentrations of L-mimosine and ammonium ferric citrate processing FaDu cells. The expressions of p-Histone-H2AX, p-ATR, p-ATM and p-mTOR proteins related to DNA damage in FaDu cells were detected by western-blot after different concentrations of L-mimosine treating FaDu cells. Results: Different concentrations of L-mimosine inhibited the growth of FaDu cells at different times. Ferric ammonium citrate of 100 µmol/L could inhibit the apoptosis of FaDu cells, while deficient or excessive iron could promote the apoptosis of tumor cells. Compared with the control group, the apoptosis rate of FaDu cells was found between L-mimosine group (200 µmol/L) was the most significant. No significant difference was found between L-mimosine (200 µmol/L)+ammonium ferric citrate (50 µmol/L) group and L-mimosine (200 µmol/L)+ammonium ferric citrate (100 µmol/L) group. Meanwhile the apoptosis rate of FaDu cells in the ferric ammonium citrate group (50, 100 µmol/L) was significantly reduced. The effect on the proliferation activity of FaDu cells, ranging from strong to weak, were L-mimosine (200 µmol/L) group, L-mimosine (200 µmol/L)+ammonium ferric citrate (50 µmol/L) group and L-mimosine (200 µmol/L)+ ammonium ferric citrate (100 µmol/L) group. Compared with the control group, the difference was significant (P<0.05). With the increase of L-mimosine concentration, the expression of p-ATR protein decreased, while p-Histone-H2AX, p-ATM, p-mTOR in FaDu cells increased. Conclusion: L-mimosine cause DNA double strand damage and lead to tumor cell apoptosis via interference with iron metabolism of FaDu cells to regulate Akt/mTOR and other signaling pathways. 2020 Vol. 50 (7): 557-562 [Abstract] ( 853 ) HTML (1 KB)  PDF (1749 KB)  ( 806 ) 563 Application value of multi-mode imaging examination based on CT and MRI in accurate diagnosis of multinodular and multicentric hepatocellular carcinoma before resection CHEN Chunmiao, CHEN Weiyue, YANG Weibin, LIN Guihan, YE Weichuan, LU Chenying, HU Xianghua, JI Jiansong DOI: 10.3969/j.issn.2095-9400.2020.07.009 Objective: To explore the application value of CT combined with MRI in accurate diagnosis of clinical multinodular and multicentric hepatocellular carcinoma before resection. Methods: A total of 42 patients with clinically diagnosed hepatocellular carcinoma in Lishui Central Hospital from May 2014 to April 2018 were collected and analyzed retrospectively. All patients underwent surgical resection and were confirmed as multinodular lesions by CT and/or MRI scan within one month before resection, and all nodules were pathologically diagnosed as hepatocellular carcinoma after resection. The diagnostic effects of CT, MRI and CT combined with MRI in the diagnosis of hepatic nodules were then compared. Results: Of 42 patients were included in the study 31 males and 11 females, with an average age of 56.0±10.4 years, 110 lesions were detected by pathological diagnosis, including 76 lesions of hepatocellular carcinoma. 89 lesions were detected by CT alone (80.90%) and 106 lesions by MRI alone (96.36%), of which 64 lesions (84.21%) and 75 lesions (98.68%)were hepatocellular carcinoma (84.21%, 98.68%). A total of 108 lesions were detected by combined examination (98.18%), and all 76 lesions of hepatocellular carcinoma were detected. According to the analysis, the detection rate of hepatocellular carcinoma lesions examined by combined examination was higher than by CT alone or MRI alone (P<0.001). The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of different examination methods were all correlated with the diameter of the tumor, the larger, the higher all the indicators. When the diameter was larger than 2 cm, the diagnostic accuracy of CT or MRI alone was consistent with that of pathological examination of liver cancer lesions. Conclusion: CT combined with MRI is better than single examination in the detection rate and diagnostic accuracy of hepatocellular carcinoma lesions and small lesions, and therefore has good clinical application value in the accurate diagnosis of multinodular and multicentric hepatocellular carcinoma before resection. 2020 Vol. 50 (7): 563-567 [Abstract] ( 840 ) HTML (1 KB)  PDF (1536 KB)  ( 867 ) 568 The role of TLR9 and CD40 signaling axis in regulation of IL10+ CD3- subsets from PBMC of children ITP patients ZHENG Mengmeng, SHI Jiandong, ZHOU Haixia DOI: 10.3969/j.issn.2095-9400.2020.07.010 Objective: To study the effects of TLR9 combined with CD40 signal on the CD3- population and IL-10 production in the PBMCs from children with ITP. Methods: The plasma IL-10 level in 8 ITP children and healthy controls were determined by using flow cytometry based LEGENplex panel technology. PBMCs from 8 ITP children and 7 healthy controls were isolated by using human lymphocytes separation buffer, and subjected to CpG plus CD40L stimulation invitro for 5 hours. Then cells were analyzed by flow cytometry after staining with the indicated markers. Results: Compared with controls (5.677±0.808), elevated plasma IL-10 level (8.063±0.892) was observed in ITP children. The percentage of CD3- cells in PBMCs was significantly increased in both CpG plus CD40L stimulation and unstimulated group. However, percentage of CD3+ cells was decreased after stimulation. Moreover, the IL-10 production was robustly enhanced in stimulated PBMCs from ITP children. In addition, compared with unstimulated control samples, the unstimulated PBMCs samples from ITP children showed reduction in cell viability. Conclusion: The TLR9 signal together with CD40 signal is crucial for triggering both IL-10 production and cell-death in CD3- cells from children with ITP. 2020 Vol. 50 (7): 568-572 [Abstract] ( 701 ) HTML (1 KB)  PDF (2321 KB)  ( 998 ) 573 Therapeutic effect of bevacizumab combined with paclitaxel on lung cancer in mice and its effect on S100A4 and MMP-9 in cancer tissue Mao Weibo, Zhu Yiling, Yan Liping, Zhou Jiahui, Huang Yuan, Chen Guorong DOI: 10.3969/j.issn.2095-9400.2020.07.011 Objective: To investigate the therapeutic effect of bevacizumab combined with paclitaxel on C57BL/6 lung cancer mice and the effect on cancer tissues S100A4 and MMP-9. Methods: Using rat-derived Leweis lung cancer tumor strains to prepare tumor fluid, SBF level healthy C57BL/6 mice were divided into normal group (uninoculated tumor fluid), model group (inoculated tumor fluid), bevacizumab group (inoculated tumor fluid), paclitaxel group (inoculated tumor fluid), and bevacizumab combined paclitaxel group (inoculated tumor fluid), with 8 cases in each group. The normal group and the model group were intraperitoneally injected with normal saline twice a week, while bevacizumab group with 15 mg/kg bevacizumab twice a week, the paclitaxel group with 10 mg/kg paclitaxel twice a week; The combination group was intraperitoneally injected with 15 mg/kg bevacizumab and 10 mg/kg paclitaxel twice a week. All mice were killed 2 weeks later, and the eyeballs were taken for blood and weighed. The tumor inhibition rate, IL-2, IL-6, TNF-α, VEGF, S100A4 and MMP-9 contents were detected by enzyme linked immunosorbent assay, T lymphocyte subsets by flow cytometry and VEGF levels by Western blot method. Results: After treatment, the tumor weight, the content of IL-2, IL-6 and TNF-α, VEGF, S100A4 and MMP-9 in bevacizumab group, paclitaxel group and combination group was significantly lower than those in model group, the tumor inhibition rate in combination group was higher than those in bevacizumab group and paclitaxel group, while the content of IL-2, IL-6 and TNF-α, VEGF, S100A4 and MMP-9 in bevacizumab group was significantly lower than those in bevacizumab group (P<0.05). The content of CD3+, CD4+, CD8+ and CD4/CD8 in bevacizumab group, paclitaxel group and combination group was significantly different from those in model group (P<0.05). Conclusion: Bevacizumab combined with paclitaxel can inhibit the growth of C57BL/6 lung cancer in mice, improve their immune function, and reduce the level of vascular endothelial growth factor, S100A4 and MMP-9. 2020 Vol. 50 (7): 573-577 [Abstract] ( 835 ) HTML (1 KB)  PDF (1403 KB)  ( 1031 ) 578 The clinical application of three-dimensional printing technique in Schazker VI tibial plateau fracture surgery ZHENG Junju, LIN Zhou, CHEN Jiaru, NI Yueping, MOU Zhefei, CAI Leyi DOI: 10.3969/j.issn.2095-9400.2020.07.012 Objective: To investigate the 3D printing technology for preoperative planning, simulation operation and operation practice of the Schazker VI tibial plateau fractures. Methods: Clinical data of fifty-seven patients with tibial plateau fractures from January 2017 to October 2019 were analyzed retrospectively. They were divided into the 3D model group and the traditional group. The operation plan for 3D model group was design according to the 3D printed models, while the traditional group underwent traditional operation. The assessment parameters included the operation time, blood loss, fluoroscopy times and HSS score. Results: The operations of both 3D model group and the traditional group were successful. All patients were followed up for 10-16 (12.8±1.9) months. In 3D model group, the operation time [(90.7±9.3)min vs. (101.3±10.9)min], blood loss [(70.7±30.0)mL vs. (96.7±37.4)mL], fluoroscopy times [(17.9±5.3) vs. (29.9±8.1)] were all less than those in traditional group (P<0.01). But HSS score of 3D model group [(85.7±5.1) vs. (81.1±5.2)] was higher than traditional group (P<0.01). Conclusion: The 3D printing technique has the advantages of less operation time, blood loss, fluoroscopy times, but higher HSS score than the traditional operation. 2020 Vol. 50 (7): 578-581,584 [Abstract] ( 661 ) HTML (1 KB)  PDF (1888 KB)  ( 757 ) 582 Team management of ECMO professional nurses in the treatment of patients with COVID-19 XIE Qiangli, WEN Qiubao, JIN Zhi, HU Yuanyang, DONG Weihua, SUN Caixia DOI: 10.3969/j.issn.2095-9400.2020.07.013 This article summed up managerial in the process of using extra corporeal membrane oxygenation (ECMO) by professional nurses to treat COVID-19 critically ill patients. A quick, effective and safe working mode was established in the treatment of patients with COVID-19 by the construction of ECMO care team, rational allocation of human resources, optimizing workflow, enhancing pre-service training to guarantee proper treatment and safety of medical staff. 2020 Vol. 50 (7): 582-584 [Abstract] ( 885 ) HTML (1 KB)  PDF (1377 KB)  ( 1217 ) 585 The establishment of emergency training system for nurses during COVID-19 epidemic XU Duo, LIANG Shiyao, YE Yuanyuan, ZHAN Lei, SUN Tiantian, XU Xiaoqun DOI: 10.3969/j.issn.2095-9400.2020.07.014 Objective: To explore an efficient nursing training system for COVID-19 emergency. Methods: From January 21. 2020 to February 21. 2020, COVID-19 emergency nursing training program was carried out for 2 290 nurses in the First Affiliated Hospital of Wenzhou Medical University. The questionnaires were distributed regarding the need for COVID-19 emergency protection and other related knowledge and skills. The standardized nursing emergency training system included homogeneous management, closed-loop management, hospital-to-department level training additional training for key personnel, stratified training and diversified training methods. All nurses were assessed for wearing and taking off protective clothing both in the hospital and departments. After intensive training, the isolation ward nurses were re-examined. Results: The questionnaires showed that 99.77% of the nurses thought it necessary to learn COVID-19 knowledge. All nurses achieved higher scores of hospital level training re-examination in the part of wearing protective clothing than in the department-level examination, which had statistically significant difference (P<0.01). The isolation ward nurses got higher scores than others, showing statistical difference (P<0.01). Conclusion: The COVID-19 emergency training can improve the protection ability of the nursing staff. 2020 Vol. 50 (7): 585-588 [Abstract] ( 757 ) HTML (1 KB)  PDF (1389 KB)  ( 904 ) 589 Perioperative infection prevention and control strategies for emergency operating room during the epidemic period of COVID-19 SHI Ruolin, ZHANG Liqing, XU Duo, YE Yuanyuan, ZHANG Wenmiao, WANG Xiaochu, TANG Xiaofen, LU Zhongqiu DOI: 10.3969/j.issn.2095-9400.2020.07.015 Due to the epidemic of COVID-19, the operating rooms of large general hospitals have played an important role in epidemic management and emergency treatment. Emergency surgery patients did not have time to undergo complete epidemiological history and clinical investigation for the COVID-19 before the operation, which was a challenge for the prevention and control of perioperative infection in the operating room. Starting from the current situation of emergency operation during the epidemic period, we discussed the prevention and control strategies in the following aspects: preoperative evaluation, operation of negative pressure operation room, admission procedures, personnel and special materials, intra- and post-operative prevention and control, and personnel training, serving as the reference for perioperative infection prevention and control strategies for emergency operating room. 2020 Vol. 50 (7): 589-592 [Abstract] ( 974 ) HTML (1 KB)  PDF (1389 KB)  ( 765 )

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