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2020 Vol. 50, No. 4 Published: 2020-04-25   259 Clinical characteristics of patients with mild COVID-19 DAI Wei, LENG Zhefeng, XU Xiaoting, CHEN Xinmiao, YU Wenwen, LIN Hui, FANG Wenxiong, ZHENG Zhe, ZHU Dan, QIU Zhangwei, YING Songmin, DAI Yuanrong DOI: 10.3969/j.issn.2095-9400.2020.04.001 Objective: To analyze the epidemiology and clinical characteristics of patients with mild COVID-19. Methods: Retrospective analysis was performed on 11 patients with mild COVID-19 and 117 non-mild patients from multiple hospitals in Zhejiang Province from January 21 to March 6, 2020. The characteristics of epidemiology, demography, clinical manifestations and laboratory examinations were analyzed. Results: The median incubation period was 10(7-14) days in 11 patients with mild COVID-19 and 6(3-9) days in non-mild covid-19, showing significant difference (Z=-4.520, P<0.001). There were more female patients (8 cases) than male patients (3 cases) in the mild group, which was contrary to the non-mild group. The median age of mild group (28 years old) was lower than that of non-mild group (48 years old) (Z=-4.070, P<0.001). One patient had coexisting disease (9.1%) in mild group while there were 40 patients (34.2%) in non-mild group. Patients with fever were fewer in mild group than in non-mild group (36.4% vs. 73.5%, χ2=4.984, P=0.026). The proportion of patients with elevated CRP in mild group was lower than that in non-mild group (9.1% vs. 59.0%, χ2=8.184, P=0.004). The proportion of leukopenia patients in the two groups was similar. The proportion of mild patients receiving oxygen inhalation treatment and glucocorticoid treatment was lower than that of non-mild patients (36.4% vs. 96.7%, 0% vs. 6%, P<0.001). By March 6, 2020, 10 cases (90.9%) of mild patients had been cured and discharged, which was significantly higher than that of non-mild patients (18 cases, 15.4%, P<0.001). There was no death or aggravation in mild patients. The median course of the two groups was similar, but the median course of the female patients in the mild group was shorter than that of the male for 4 days. Conclusion: Mild COVID-19 patients showed imperceptible, long incubation period, mild symptoms, high CRP normal rate, normal CT, which may cause missed diagnosis and increased risk of virus transmission. Though mild patients had good prognosis, but they still need comprehensive treatment and close observation. In addition, there may be more mild patients and better prognosis in women than in men. 2020 Vol. 50 (4): 259-263 [Abstract] ( 938 ) HTML (1 KB)  PDF (1447 KB)  ( 966 ) 264 CT findings and dynamic changes of COVID-19 LIN Yizi, CHEN Li, LI Yang, LUO Min, ZHENG Jun, CEN Xiuya, LI Yongchou, DAI Tingting, LIN Dou, HE Di, REN Huai, GAO Yuantong DOI: 10.3969/j.issn.2095-9400.2020.04.002 Objective: To study the CT findings and dynamic imaging changes of COVID-19. Methods: A total of 60 patients with COVID-19 from the Third Affiliated Hospital of Wenzhou Medical University from January 26, 2020 to March 15, 2020 were included. All patients underwent sequential CT examinations with an interval of 2-10 days. The CT features in the initial, progressive and dissipating stage were analyzed. Results: In the initial stage, 21 cases had small ground-glass opacities accounting for 75.0% and, 7 cases nodules accounting for 25.0%. In progressive stage, ground-glass opacities accompanied by consolidations were found in 49 cases (81.7%), prominent consolidations in 11 cases (18.3%). Multiple lesions were found in 52 cases (86.7%), 44 cases (73.3%) were found lesions in the middle and lower lobes of both lungs, and 55 case (91.7%) were mainly located under pleura. In dissipation stage, the number of lesions decreased in 49 cases (81.7%), the range decreased in 60 cases (100%) and the density decreased in 60 cases (100%). Ground-glass change were observed again in 36 cases (60.0%) and 24 cases (40.0%) had strip solid change. After absorption, 9 cases (15.0%) had residual subpleural arc shadow and interlobular septal thickening. Conclusion: CT is able to distinctly demonstrate the distribution, density, shape, and range of pulmonary lesions of COVID-19, and display pulmonary fibrosis as well. Therefore, CT can be used to display the early abnormalities and dynamic changes of COVID-19. 2020 Vol. 50 (4): 264-267，271 [Abstract] ( 1036 ) HTML (1 KB)  PDF (1648 KB)  ( 1053 ) 268 Reflection on the construction of public health talents in crisis of COVID-19 outbreak HUANG Hong, LIU Xiaodong DOI: 10.3969/j.issn.2095-9400.2020.04.003 In the prevention and control of coronavirus disease 2019 (COVID-19) epidemic, China's public health system plays an important role, but there exist at the same time the quantity and quality problems of public health professionals. In this study, the professional team of China’s public health in the current system was analyzed from the perspectives of education, employment, and keeping possession of talents. The authors put forward the ideas of reform in the following aspects: the scale of undergraduates should be expanded and the quality of secondary training improved; public health talents should be used properly; public health professionals should be retained by raising their salary and sense of achievement. 2020 Vol. 50 (4): 268-271 [Abstract] ( 909 ) HTML (1 KB)  PDF (1417 KB)  ( 899 ) 272 Survey of order-oriented general medical students’ participation in the prevention and control of COVID-19 epidemic and its impact on their professional self-identity ZHOU Peisen, YANG Chenbin, LI Zhangping, LI Xiaokun, ZHAO Wenjuan, XIN Chengyuan, LIN Jin, WU Huilei, ZHU Xuebo DOI: 10.3969/j.issn.2095-9400.2020.04.004 Objective: To investigate the order-oriented general medical students’ participation in the prevention and control of COVID-19 and its influence on their professional self-identity, which may serve as a support for the targeted development of professional identity in the education of future general medical practitioners. Methods: A questionnaire was developed according to the literature and guidelines, which included basic information of the respondents, acquisition of covid-19 epidemic information, participation in epidemic resistance, and the impact of the epidemic on professional self-identity. The questionnaire was conducted randomly students from Wenzhou Medical University, Jilin Medical University and Yunnan University of Chinese Medicine. Univariate analysis and multivariate analysis were used to analyze the factors in the COVID-19 epidemic that impact on their professional self-identity. Results: Totally 3 321 valid questionnaires were collected. Of all the respondents, 40.6% (1 347/3 321) were male and 59.4% (1 974/3 321) female; 6.0% (198/3 321) were CPC members and 94.0% (3 123/3 321) were not; 64.1% (2 129/3 321) were juniors and 35.9% (1 192/3 321) seniors; 70.6% learned about COVID-19 for over 30 minutes; 72.4% learned about COVID-19 voluntarily; 92.4% had read state-published COVID-19 guidelines; 59.0% showed willingness to sign up in first-line fight against the epidemic; 23.6% had participated in this epidemic prevention and control; the epidemic had a positive impact on 87.7% students’ professional self-identity. Univariate analysis and multivariate analysis showed that grade (OR=1.653, 95%CI=1.314-2.080, P<0.001), learning time (OR=0.633, 95%CI=0.500-0.803, P<0.001) and method (OR=1.750, 95%CI=1.380-2.220, P<0.001) about COVID-19, reading national guidelines for COVID-19 (OR=2.264, 95%CI=1.630-3.144, P<0.001), willingness to participate (OR=7.334, 95%CI=5.529-9.729, P<0.001), actual participation in this epidemic prevention (OR=1.543, 95%CI=1.101-2.12, P=0.012) were independent factors influencing their professional self-identity. Conclusion: In the COVID-19 epidemic, the majority of students can learn the information concerned voluntarily, participate in epidemic prevention and control to raise their sense of professional identity. It is suggested that the order-oriented general medical students, especially seniors, should be engaged themselves more in major public health events, take the initiative to learn relevant information from professional authorities, and promote their willingness and actual participation in the major public health events. 2020 Vol. 50 (4): 272-277，284 [Abstract] ( 963 ) HTML (1 KB)  PDF (1570 KB)  ( 1248 ) 278 Construction essentials of “makeshift specialized hospital for infectious diseases”: A discussion based on COVID-19 epidemic situation CHEN Yunyu, XU Duo, LIANG Shiyao, XU Xiaoqun, YE Yuanyuan DOI: 10.3969/j.issn.2095-9400.2020.04.005 The COVID-19 epidemic has attracted global attention due to the highly infection of the virus, which led to the rapid spread and large-scale outbreak. While dealing with such a sudden and rapid pandemic disease, it is very essential to quickly build a temporary specialized hospital for the emergency use, which should be highly safety to meet demands of the treatment of patients, the protection of medical staff and the control of the infectious disease. Combined with the construction experience of the three makeshift specialized hospitals (i.e. Xiao Tangshan in Beijing, Huoshenshan and Leishenshan in Wuhan) and the renovated emergency treatment center of infectious diseases in the First Affiliated Hospital of Wenzhou Medical University, this article clarifies the construction essentials including hospital site selection, planning and design, hvac system, water supply and drainage system, medical gas requirements and the medical waste disposal and elaborates the key points. 2020 Vol. 50 (4): 278-284 [Abstract] ( 1046 ) HTML (1 KB)  PDF (2081 KB)  ( 1214 ) 285 The effect of Hyp-PDT on K562 activity and autophagy pathway of leukemia cells cultured in vitro WANG Dexuan, WANG Wantie, ZHENG Lyuzhen, CHEN Weiwei, YANG Qing, ZHUANG Jieqiu, XU Yixiao DOI: 10.3969/j.issn.2095-9400.2020.04.006 Objective: To explore the effect of Hyp-PDT (Hypericin-mediated photodynamic therapy) on K562 activity and autophagy pathway of leukemia cells cultured in vitro. Methods: Human leukemia cell line K562 was cultured in vitro. Hyp-PDT was performed with 0.3 mW/cm2 light intensities and 4 min irradiation times. The CCK8 analysis was applied and the results were analyzed. Cell protein was collected instantly before irradiation, 4 h, 8 h and 16 h after irradiation respectively for Western blot determination of autophagy related protein. Morphological changes of K562 were observed under phase contrast microscope and electron microscopy. Results: Hyp-PDT with the light intensity of 0.3 mW/cm2 significantly decreased cell viabilities in K562 post irradiation at different time points (all P<0.01). After Hyp-PDT intervention, some cells in hypericin group showed swelling, degeneration and even dissolution after light irradiation. There were several cases of autophagy in K562 cells before irradiation, 8 h after irradiation, and autophagy increased in cells treated with DMSO but decreased obviously or even disappeared in Hyp group. The expression of LC3 protein decreased in Hyp group but increased slightly in DMSO group 8 h and 16 h after irradiation. The expression of Beclin-1 decreased 16 h after Hyp-PDT and a synchronous decrease of p-Akt protein was observed. Conclusion: Hyp-PDT of K562 in vitro can promote cell fatality by inhibiting autophagy in K562 cells. It may be related to the inhibition of Akt pathway. 2020 Vol. 50 (4): 285-289 [Abstract] ( 803 ) HTML (1 KB)  PDF (1719 KB)  ( 944 ) 290 Interaction between BMI and lipid profile in diabetic kidney disease in Chinese Han population with type 2 diabetes YU Weihui, HU Xiang, CHEN Xiong, ZHOU Qi, DENG Huihui, GU Xuejiang DOI: 10.3969/j.issn.2095-9400.2020.04.007 Objective: To explore the interaction between BMI and lipid profile in diabetic kidney disease (DKD) in Chinese Han population with type 2 diabetes. Methods: We recruited 647 unrelated Han Chinese participants in this study, including 283 DKD patients and 364 non-DKD patients. Binary logistic regression was applied to analyze the association between lipid profile and DKD, and the interaction between BMI and lipid profile. Results: TG (OR=1.446, 95%CI: 1.076-1.942, P=0.014), nonHDL-C (OR=1.961, 95%CI: 1.011-3.804, P=0.046), nonHDL-C/HDL-C (OR=1.804, 95%CI: 1.134-2.869, P=0.013), TC/HDL-C (OR=2.293, 95%CI: 1.234-4.260, P=0.009), TG/HDL-C (OR=1.345, 95%CI: 1.057-1.712, P=0.016) were all significantly associated with DKD, ORs were calculated for the logarithmically transformed indexes. Significant interactions between BMI and TG, nonHDL-C/HDL-C, TC/HDL-C, TG/HDL-C were detected (P values equal to 0.036, 0.035, 0.034, 0.016, respectively). Conclusion: It is indicated that TG, nonHDL-C, nonHDL-C/HDL-C, TC/HDL-C, TG/HDL-C are significant risk factors for DKD in Chinese patients with type 2 diabetes. BMI modulated the association between lipid profile and DKD in this population. 2020 Vol. 50 (4): 290-294，299 [Abstract] ( 864 ) HTML (1 KB)  PDF (1544 KB)  ( 856 ) 295 The effect of surgical sperm extraction timing on the clinical outcomes of intracytoplasmic sperm injection PAN Chengshuang, ZHANG Huan, XU Zhihui, JIN Jianyuan, FEI Qianjin, NI Wuhua DOI: 10.3969/j.issn.2095-9400.2020.04.008 Objective: To investigate the effect of surgical sperm extraction timing on the clinical outcomes of  intracytoplasmic sperm injection (ICSI). Methods: Totally 839 cases of surgically obtained sperm were analyzed for fresh cycle of ICSI treatment. According to the operation time, they were divided into ICSI group on the day of sperm extraction and ICSI group on the next day. The fertilization rate, cleavage rate, high quality embryo rate, implantation rate, clinical pregnancy rate, abortion rate and other results were compared between the two ICSI groups. Results: Embryo culture in vitro and clinical results showed that there was no significant difference in fertilization rate, maturation rate, cleavage rate, D3 quality embryo rate, implantation rate, clinical pregnancy rate and abortion rate between ICSI group on the day of surgical sperm extraction and ICSI group on the next day (P>0.05). Conclusion: The surgical sperm extraction time has no significant effect on the clinical outcomes of intracytoplasmic sperm injection. 2020 Vol. 50 (4): 295-299 [Abstract] ( 740 ) HTML (1 KB)  PDF (1423 KB)  ( 814 ) 300 The knockout of nuclear factor E2 related factor and its effect on myofibroblast activation and renal fibrosis CHENG Shuibing, GUO Yangyang, ZHU Hengyue, XIAO Yanyi, ZHENG Shizhang, QI Ruyi, ZHOU Junlei, YANG Mei, BAI Yongheng DOI: 10.3969/j.issn.2095-9400.2020.04.009 Objective: To investigate the knockout of nuclear factor E2 related factor (Nrf2) and its effect on the activation of renal myofibroblasts and interstitial fibrosis. Methods: Mice were randomly divided into four groups: Nrf2-wild-type sham group, Nrf2-knock-out sham group, Nrf2-wild-type unilateral ureteral obstruction (UUO) group and Nrf2-knock-out UUO group, with 6 mice in each group. The levels of serum creatinine and urea nitrogen were determined by biochemical analyzer; Periodic acid-Schiff staining (PAS) was used to observe the degree of renal damage; Masson staining was used to evaluate the accumulation of total collagen in renal interstitium; immunohistochemical staining was used to analyze the expression levels of myofibroblastic marker α-SMA, matrix component type III collagen and transforming growth factor β1 (TGF-β1); qRT-PCR was used to detect the mRNA expression of TGF-β1. Results: Compared with the Nrf2-wild-type sham group, the serum creatinine and urea nitrogen levels in the Nrf2-wild-type UUO group were significantly increased (P<0.05). There was no significant difference in serum creatinine and urea nitrogen levels between the Nrf2-wild-type UUO group and the Nrf2-knock-out UUO group (P>0.05). PAS staining showed that renal tissue damage was significantly aggravated in the Nrf2-knock-out UUO group (P<0.01), compared with the Nrf2-wild-type UUO group; Masson staining showed that total accumulation of renal interstitial collagen in the Nrf2-knock-out UUO group was significantly increased (P<0.01), compared with the Nrf2-wild-type UUO group; immunohistochemical staining showed that the expression of α-SMA and type III collagen in renal tissue of Nrf2-knock-out UUO group was significantly increased (P<0.05), compared with the Nrf2-wild-type UUO group, which was related to TGF-β1 mRNA and protein expression caused by Nrf2 knockout (P<0.05). Conclusion: Nrf2 knockout can aggravate the degree of renal injury and fibrosis caused by ureteral obstruction. Its mechanism may be related to Nrf2 knockout that increases TGF-β1 levels, resulting in excessive activation of myofibroblasts and collagen accumulation in renal interstitium. 2020 Vol. 50 (4): 300-305，311 [Abstract] ( 739 ) HTML (1 KB)  PDF (1991 KB)  ( 753 ) 306 Mitochondrial haplogroup analysis of 17 Leber’s hereditary optic neuropathy patients carrying MT-ND1 3460G>A mutation CHEN Jie, CI Xiaorui, ZHANG Juanjuan, GUAN Minxin DOI: 10.3969/j.issn.2095-9400.2020.04.010 Objective: To study the effect of mitochondrial haplotype on the penetrance of LHON among 17 probands with m.3460G>A mutation. Methods: LHON samples of 1 218 cases were screened for m.3460G>A mutation before the whole mitochondrial DNA and mitochondrial haplotype were analyzed. Results: We found 17 LHON pedigrees carrying the m.3460G>A mutation, accounting for 1.4% (17/1 218) in the Chinese LHON cohort. Meanwhile, 17 haplogroups were found to be mitochondrial subtype A, B5b, B5b2, C4a1, D4, D5b1, F1, F1a1, H2, M7b1, M7b2, M7c1, M8a2, M10a, M12, respectively. Three of all were haplogroup D5b1 and the others had only one proband. Eleven probands were haplogroup M and six haplogroup N. The former had more probands in number and the degree of visual loss was more severe than the latter. Conclusion: Haplogroup M is more prone to visual loss than haplogroup N. 2020 Vol. 50 (4): 306-311 [Abstract] ( 777 ) HTML (1 KB)  PDF (1690 KB)  ( 953 ) 312 A comparative study of amoxicillin sulbactam and cefuroxime in the prevention of infection during periop-erative period of permanent pacemaker NI Qiuming, Lin Miao, JI Xiaojun, Lin Bin, Wang Jun DOI: 10.3969/j.issn.2095-9400.2020.04.011 Objective: To investigate the effectiveress and safety of different antibiotics in the prevention of infection during perioperative period of permanent pacemaker, for the sake of reasonable selection of antibiotics in clinical practice. Methods: From August 2015 to August 2017, 120 patients with permanent pacemaker implantation in Wenzhou Central Hospital were randomly divided as experimental group and control group, each group consisting of 60 patients. Amoxicillin sulbactam (1.5 g intravenous drip) was used half an hour before operation in experimental group, and cefuroxime (1.5 g intravenous drip) was used in control group to prevent infection within 24 hours after operation. In both groups, body temperature, white blood cell (WBC), C-reactive protein (CRP) and procalcitonin (PCT) were measured before operation, 48 hours and 72 hours after operation and the indexes were compared between the two groups. The incidence of fever, pacemaker bag infection, bag hematoma, pulmonary infection, infective endocarditis, pathogens, adverse reactions of antibiotics and cost of antibiotic were compared within 7 days after operation. Results: There was no significant difference in body temperature, WBC, CRP and PCT between the two groups before operation (P>0.05). The body temperature, WBC, CRP and PCT of the experimental group at 48 and 72 hours after operation were significantly lower than those of the control group (P<0.05). The incidence of fever within 7 days in the experimental group was lower than that of the control group (1.67% vs. 11.67%, P<0.05). The incidence of pacemaker bag infection in the experimental group was lower than that in the control group (3.33% vs. 13.33%, P<0.05). The incidence of pocket hematoma, pulmonary infection and infectious endocarditis was not significantly different between the two groups (P>0.05). In the control group, 1 case of severe infection required removal of pacemaker and electrodes, but none in the experimental group. Staphylococcus aureus was the most common pathogen of pacemaker infection in the two groups, while Staphylococcus epidermidis, Escherichia coli and Klebsiella pneumoniae were cultured in the control group. No adverse reactions were found in the experimental group. There was no significant difference between the two groups (P>0.05). The cost of antibiotics in the experimental group was less than that in the control group (P<0.05). Conclusion: Amoxicillin sulbactam is superior to cefuroxime in the prevention of infection during perioperative period of permanent pacemaker implantation with lower incidence of infection, less cost and comparable adverse reactions to cefuroxime. 2020 Vol. 50 (4): 312-316 [Abstract] ( 827 ) HTML (1 KB)  PDF (1456 KB)  ( 906 ) 317 IL-27 regulates LPS-induced acute pneumonia in mice through STAT3 signaling pathway WU Xia, REN Yan, WU Huimin DOI: 10.3969/j.issn.2095-9400.2020.04.012 Objective: To investigate the role of IL-27 in lipopolysaccharide (LPS)-induced acute pneumonia in mice by regulating STAT3 signaling pathway. Methods: A total of 32 male mice were randomly divided into normal control group, LPS group, LPS+IL-27 group and LPS+IL-27 antibody group, with 8 mice in each group. After anaesthesia, the lungs of mice were taken and staining was used to observe the lung injury of mice in each group by HE. The mRNA expression levels of STAT3 and SOCS3 in lung tissue and serum of mice in each group were detected by qRT-PCR. Western blot method was used to detect the protein expression levels of STAT3, p-STAT3 and SOCS3 in lung tissue of mice in each group. The expression levels of TNF-α, IL-1β, IL-10 and TGF-β1 in lung homogenate and serum were detected by ELISA. Results: HE staining showed that in the normal control group, LPS group and LPS+IL-27 antibody group, the alveolar structure was destroyed, the alveolar wall was diffusely thickened, and there was obvious inflammatory cell infiltration. The pathological changes and inflammatory cell infiltration of LPS+IL-27 group were less than those of LPS group. Compared with the normal control group, the expression levels of STAT3 and p-STAT3 protein in lung tissue of mice in LPS group and LPS+IL-27 antibody group was significantly increased (P<0.05); the expression level of SOCS3 in LPS+IL-27 group was significantly higher than in LPS+IL-27 antibody group (P<0.05). The expression levels of TNF-α and IL-1β in lung tissue and serum of mice in each group were significantly higher than those in the normal control group (P<0.05). The expression levels of TNF-α and IL-1β in LPS+IL-27 group were significantly lower than those in LPS group and LPS+IL-27 antibody group (P<0.05). The level of IL-10 expression in LPS group and LPS+IL-27 antibody group was significantly higher than that in normal control group (P<0.05). The expression levels of IL-10 and TGF-β1 in LPS+IL-27 group was significantly higher than those in LPS+IL-27 antibody group (P<0.05). The results of immunohistochemistry showed that compared with the normal control group, TNF-α was mainly expressed in the cytoplasm of LPS group and LPS+IL-27 antibody group, while IL-1β was mainly expressed in the membrane and cytoplasm. Western blot showed that TNF-α and IL-1β were were highly expressed in lung tissue (P<0.05); the expression level of TNF-α and IL-1β in LPS+IL-27 group was significantly lower than that in LPS group and LPS+IL-27 antibody group (P<0.05). Conclusion: Exogenous IL-27 may improve LPS-induced acute pneumonia in mice by inhibiting the phosphorylation of STAT3 signaling pathway related proteins. 2020 Vol. 50 (4): 317-322,327 [Abstract] ( 882 ) HTML (1 KB)  PDF (2007 KB)  ( 939 ) 323 ROC curve-based study of the screening criteria for the unrelated hematopoietic stem cell donors TU Xiaolian, YANG Ting, PENG Fang, SUN Yudan, ZHANG Guohua, DING Na’ni DOI: 10.3969/j.issn.2095-9400.2020.04.013 Objective: To determine the criteria for screening the unrelated hematopoietic stem cell donors  based on the receiver operating characteristic curve (ROC curve). Methods: A questionnaire survey was conducted between 2007 and 2018 in Wenzhou City to collect information from 40 donors who donated hematopoietic stem cells and 166 potential donors in order to construct a ROC curve between donation cognition, donation willingness and donation attitude. Results: A joint screening method integrating the degree of donation cognition, donation willingness and donation attitude were used. The area under the ROC curve was 0.87 (95%CI: 0.81-0.93), with sensitivity being 70.00% and specificity 90.96%. The consistent rate was 86.89% and the Youden index was 0.61. Conclusion: To promote the possibility of donation and reduce the refusal rate, it is helpful to enhance the awareness of unrelated hematopoietic stem cell donors, while increasing their willingness and attitude towards hematopoietic stem cell donation. 2020 Vol. 50 (4): 323-327 [Abstract] ( 682 ) HTML (1 KB)  PDF (1497 KB)  ( 764 ) 328 A compositional analysis of adult nasal septal cartilage XU Haiting, HU Kepeng, XUE Jixin, SHI Li, SONG Yonghuan, LI Xiaoyang DOI: 10.3969/j.issn.2095-9400.2020.04.014 Objective: To localize quantitatively the major biochemical constituents of human adult nasal septal cartilage, which may provide an experimental basis and theoretical guidance for clinical nasal septal cartilage transplantation. Methods: Seven adult corpse nasal septal cartilage specimens were obtained, each divided into 5 regions, labeled as A, B, C, D, and E respectively. Biochemical analysis was used to determine the relative wet weight of the main components of the septal cartilage: chondrocytes, collagen and sulfated glycosaminoglycans (sGAG). Results: Each milligram of wet cartilage fragment contains an average of (22 900±2 900) cells, (60.3±6.4) μg of collagen, and the average content of sulfated glycosaminoglycans (sGAG) was (14.6±3.5) μg per mg of wet weight. The number of cells and collagen did not change significantly in each region. Compared with region A and region C, the sGAG content in B and D regions was significantly increased (P<0.05). Compared with region A and region C, the ratio of collagen to sGAG in B, D and E regions was significantly decreased (P<0.05). Conclusion: The content of sGAG shows specific regional changes in the septal cartilage of the nasal septum. The local morphology of the sGAG deposition is consistent with the predominantly retained L-pillar shape in rhinoplasty or nasal reconstruction. 2020 Vol. 50 (4): 328-330,333 [Abstract] ( 697 ) HTML (1 KB)  PDF (1498 KB)  ( 924 ) 334 The effect of dual-use nipple protection and milk feeder on the success rate of breast feeding in the parturient women with depressed nipple XU Huifang, LI Yao, ZHANG Liping DOI: 10.3969/j.issn.2095-9400.2020.04.016 Objective: To investigate the effect of dual-use nipple protection and milk feeder on the success rate of breast feeding in the parturient women with depressed nipple. Methods: Eighty parturient women with type II sunken nipple delivered in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University were randomly divided into control group (n=40) and observation group (n=40) from October 1st, 2017 to October 30th, 2018. The sunken nipple of parturient women in the control group was corrected by routine nursing method, while the sunken nipple of parturient women in the observation group was corrected by self-made dual-use nipple protection and milk feeder. The breast-feeding rate in the 3rd day after delivery, the scores of S-AI (state anxiety) and T-AI(trait anxiety) on the 3rd day after delivery were compared between two groups. Results: The breast feeding rate on the 3rd day postpartum in the parturient women of the observation group was significantly higher than that of the control group (P<0.05), and the levels of S-AI and T-AI on the 3rd day postpartum in the parturient women of the observation group were significantly lower than those of the control group (P<0.05). Conclusion: The dual-use nipple protection and milk feeder is proved to lower the score of S-AI and T-AI and increase the breast feeding rate, thus promoting the health of mother and infant. 2020 Vol. 50 (4): 334-336 [Abstract] ( 652 ) HTML (1 KB)  PDF (1463 KB)  ( 848 )

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