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2020 Vol. 50, No. 2 Published: 2020-02-25   87 Characteristics of transcripts and changes of UL111A gene during the productive phase of infection in HCMV Liu Xin, Xie Wangkai, Sun Xiangwei, Chen Zhiyuan, Shen Xian, Xue Xiangyang DOI: 10.3969/j.issn.2095-9400.2020.02.001 Objective: To investigate the characteristics of transcripts and changes of human cytomegalovirus (HCMV) UL111A gene at different time points during the productive phase of infection. Methods: HCMV AD169 strain was used to infect human foreskin fibroblast (HFF) at various times of 12 h, 24 h, 48 h, 3 d, 5 d, 7 d, 9 d. Then RNA was extracted, reverse transcribed into cDNA, and the characteristics of UL111A transcript were detected by PCR. The amplified products were verified by cloning and sequencing to characterize the changes of UL111A transcripts at different time points. Then eukaryotic expression vector pcDNA3.1 was used to construct the three transcripts coding region sequence vectors of UL111A which were pcDNA3.1-cmvIL-10, pcDNA3.1- LAcmvIL-10 and pcDNA3.1-unspliced; the efficient expression of the constructed coding region vectors in 293T cells was confirmed by Western blot. Results: CmvIL-10, LAcmvIL-10 and unspliced transcripts could be detected in HFF cells which infected with HCMV AD169 strain. The unspliced transcript accounted for the most, and the cmvIL-10 transcript accounted for the least. All three transcripts could be detected at 24 h and 9 d after infection, while cmvIL-10 transcript was not detected at other time points. Furthermore, the coding region sequences of the three transcripts could all be efficiently expressed in 293T cells. Conclusion: HCMV UL111A gene has three transcripts at different time points during the proliferative infection. 2020 Vol. 50 (2): 87-91 [Abstract] ( 863 ) HTML (1 KB)  PDF (1642 KB)  ( 924 ) 92 Dexmedetomidine alleviates proliferation of neural stem cells from corticosterone-induced injury and its mechanisms PAN Yizhao, MA Junmei, HUANG Jianjian, HU Zhiyan, MEI Hongxia, LIN Han DOI: 10.3969/j.issn.2095-9400.2020.02.002 Objective: To investigate the alleviating effect of dexmedetomidine (DEX) on the proliferation of neural stem cells (NSCs) from corticosterone (CORT)-induced injury and explore the mechanisms potentially involved. Methods: NSCs were harvested from E15 SD rat brain and cultured. Neuroepithelial stem cell protein (nestin and SOX2) staining was identified. Whether NSCs express mRNA of the α-2A, α-2B, α-2C adrenoceptor subtypes was detected by Reverse transcriptase-PCR. The NSCs were randomly divided as the Control, DMSO, CORT (0.01, 0.1, 1 μmol/L), Dexmedetomidine with CORT. The NSCs in the Dexmedetomidine with CORT group were pretreated with different concentrations of dexmedetomidine (0.01, 0.1, 1 μmol/L) for 1 h prior to incubation with 1 μmol/L CORT for 24 h. Cell cytotoxicity and Edu assays were used to figure out the optimal concentration of dexmedetomidine which can be used for the protein studies; Protein levels of GSK-3β, p-GSK-3β, β-catenin and Cyclin D1 were quantified with Western blot. Results: More than 98% of them were NESTIN/SOX2 positive. The three subtypes of α-2 receptor were expressed on NSCs. There was no significant difference between each group in the release of LDH. CORT significantly decreased NSCs proliferation, however, 1 μmol/L DEX increased NSCs proliferation. CORT inhibited p-GSK-3β, β-catenin and Cyclin D1 expression compared with DMSO group, but 1 μmol/L DEX pretreatment enhanced p-GSK-3β, β-catenin, Cyclin D1 expression, compared with CORT group. Total GSK-3β levels in each group did not change significantly. The protective effect of dexmedetomidine on CORT-exposed NSCs could be partly reversed by PI3K phosphorylation inhibitor LY294002. Conclusion: Dexmedetomidine may protect NSCs from corticosterone-induced injury via the GSK-3β/β-catenin signaling pathway. 2020 Vol. 50 (2): 92-97 [Abstract] ( 809 ) HTML (1 KB)  PDF (1836 KB)  ( 757 ) 98 Effect of curcumin in pulmonary hypertension rats by NLRP3/Caspase-1/IL-1β axis CAI Xueding, CHEN Mayun, LI Wenya, WU Peiliang, YAO Dan, HUANG Xiaoying DOI: 10.3969/j.issn.2095-9400.2020.02.003 Objective: To investigate the therapeutic effect and mechanism of curcumin in hypoxia-induced pulmonary hypertension (HPH). Methods: Pulmonary artery smooth muscle cells (PASMCs) and Sprague-Daeley (SD) rats were studied in vitro and in vivo respectively. The PASMCs and SD rats were divided into 3 groups: normoxia group (N), hypoxia group (H), hypoxia+curcumin group (HC). The activity of PASMCs cells in each group was measured by cck-8, and the expressions of NLRP3, Caspase-1 and il-1β protein in PASMCs were detected by Western blot. The average pulmonary artery pressure and the degree of right cardiac hypertrophy in each group of rats were measured by hemodynamics, while the degree of pulmonary vascular remodeling in each group of rats was observed by HE staining, and the expression difference of the above-mentioned proteins in the lung homogenate in each group of rats was detected by western blot. Results: Compared with the N group, the quantity of PASMCs in H group were significantly increased. The NLRP3/Caspase-1/IL-1β protein expression in H group was increased. The mPAP and right cardiac hypertrophy index in group H were significantly increased, and the pulmonary vascular remodeling was obvious. Compared with the H group, the quantity of PASMCs in HC group were significantly decreased, and the expression of NLRP3/Caspase-1/IL-1β protein in PASMCs and lung homogenate was decreased, mPAP and right cardiac hypertrophy index were significantly decreased in HC group, and pulmonary vascular remodeling was improved. Conclusion: Curcumin could inhibits pulmonary arterial hypertension, pulmonary vascular remodeling and right ventricle hypertrophy via suppressing NLRP3/Caspase-1/IL-1β axis mediated inflammatory reaction. 2020 Vol. 50 (2): 98-101，107 [Abstract] ( 982 ) HTML (1 KB)  PDF (1679 KB)  ( 899 ) 102 The effect of cerebral ischemia reperfusion in different time on autophagy of hippocampal neurons and PI3K/mTOR pathway in rats SHAO Jin’gen, YANG Jiang, CHEN Haohao, TAO Hongmiao, LI Xusheng DOI: 10.3969/j.issn.2095-9400.2020.02.004 Objective: To investigate the effect of cerebral ischemia reperfusion in different time on autophagy of hippocampal neurons and PI3K/mTOR pathway in rats. Methods: Totally 72 rats were randomly divided into sham operation group and cerebral ischemia reperfusion groups (reperfusion for 0 h, 6 h, 12 h, 24 h, 36 h, respectively), with 12 rats in each group. The rat model of global cerebral ischemia reperfusion injury was established by four-vessel occlusion. Modified neurological severity score was used to evaluate hippocampal neurological function in rats, HE staining was used to detect the damage of hippocampal neurons in rats, the autophagic bodies in hippocampal neurons were observed under transmission electron microscopy, Western blot was used to detect the expressions of Beclin-1, LC3 II, p62, p-PI3K, p-Akt, and p-mTOR proteins. Results: Compared with the sham operation group, the cerebral ischemia reperfusion rats showed an increase in the modified neurological severity score, cerebral infarction area, and the number of autophagosomes; the expressions of autophagy-related proteins Beclin-1 and LC3 II in hippocampus of rats were significantly up-regulated (P<0.05), the expression of p62 was significantly down-regulated (P<0.05), the expressions of PI3K/mTOR pathway related proteins p-PI3K, p-Akt, and p-mTOR were significantly down-regulated (P<0.05). With the prolongation of cerebral ischemia reperfusion time, the expressions of autophagy-related proteins Beclin-1 and LC3 II increased, the expression of p62 decreased and so did the PI3K/mTOR pathway related proteins p-PI3K, p-Akt, and p-mTOR. Conclusion: Cerebral ischemia reperfusion can enhance autophagy of hippocampal neurons and inhibit PI3K/mTOR signaling pathway in rats. 2020 Vol. 50 (2): 102-107 [Abstract] ( 877 ) HTML (1 KB)  PDF (1996 KB)  ( 757 ) 108 The sensitization effects of sub-toxic concentration of 5-hydroxy-4’-nitro-7-propionyloxy-genistein on radiotherapy for osteosarcoma cells and its possible mechanism ZHAO Xuezheng, Gao Wei, TANG Baoyong, ZHENG Sihua, LI Lijun DOI: 10.3969/j.issn.2095-9400.2020.02.005 Objective: To investigate the sensitization effect of sub-toxic concentration of 5-hydroxy- 4’-nitro-7-propionyloxy-genistein (HNPG) on radiotherapy for human osteosarcoma Saos-2 cells and its possible molecular mechanism. Methods: Saos-2 cells were cultured in vitro, and the inhibiting effect of sub-toxic concentration of HNPG and the sub-toxic dose of X-ray on Saos-2 cells were selected using MTT assay. The sensitization effect of HNPG on X-ray induced apoptosis were observed using flow cytometry with annexin V-FITC (AV) and propidium iodide (PI) staining. The sensitization effect of HNPG on X-ray regulating reactive oxygen species (ROS) were measured in flow cytometry with 2’, 7’‑dichlorodihydrofluorescein diacetate (DCFHDA) staining. The sensitization effect of HNPG on X ray regulating Mitochondrial Membrane Potential (MMP) were measured in flow cytometry with lipophilic cationic dye 2 (6 Amin 3 imino 3H xanthen 9 yl) benzoic acid methyl ester (Rh123) staining. The bioactivity of superoxide dismutase (SOD) and catalase (CAT) and the content of glutathione (GSH) and malondialdehyde (MDA) were detected by Enzyme-linked immunosorbent assay (Elisa). The related apoptotic proteins, such as bcl-2, bax, cyt-c and cleaved-caspase-3 were assessed using Western blot. Results: The sub-toxic concentration of HNPG could dramatically enhance the anti-tumor activity of the sub-toxic dose of X-ray against Saos-2 cells in vitro, inhibited the cells proliferation, induced the cells apoptosis, accompanied by the bioactivity of SOD, declined CAT, reduced content of GSH, and enhanced content of MDA. Simultaneously the content of ROS was increased and the level of MMP was cut down, accompanied by the up-regulated proteins of bax, cyt-c and cleaved-caspase-3 and down-regulated bcl-2 protein. Conclusion: The sub-toxic concentration of HNPG could sensitize the inhibiting proliferative effect of the sub-toxic dose of X ray in Saos-2 cells in vitro, which might be related to the decreased bioactivity of SOD and CAT, reduced content of GSH, accumulated ROS in cells, damaged integrity of mitochondrial membrane and induced cells apoptosis. 2020 Vol. 50 (2): 108-114 [Abstract] ( 787 ) HTML (1 KB)  PDF (1626 KB)  ( 921 ) 115 The value of acoustic radiation force impulse imaging in the quantitative evaluation of the elasticity of triceps surae with venous plexus dilatation DONG Yanyan, SUN Xielu, WANG Pengfei, XU Maosheng, ZOU Chunpeng DOI: 10.3969/j.issn.2095-9400.2020.02.006 Objective: To investigate the elastic changes of triceps surae with venous plexus dilatation by acoustic radiation force impulse imaging (ARFI). Methods: A total of 74 subjects who underwent double-limb venous ultrasonography at the Second Affiliated Hospital of Wenzhou Medical University from October 2017 to June 2018 were collected. Patients with triceps surae venous plexus dilatation were selected as the case group, in which a total of 48 patients (66 legs) were collected. According to the extent of venous plexus dilatation involvement, the case group was divided as Group One, in which patients had venous plexus dilatation in both medial gastrocnemius (MG) and soleus and Group Two, in which patients only had soleus venous plexus dilatation, with each group having 24 patients (33 legs). Twenty-six individuals (fifty two legs) were collected as the control group who didn’t have venous plexus dilatation in either gastrocnemius or soleus. All subjects underwent ARFI imaging in virtual touch tissues quantification (VTQ) model to obtain the value of shear wave velocity (SWV) of triceps surae which contains MG, lateral gastrocnemius (LG) and soleus (SOL) at three different positions including the natural position, the maximum metatarsal flexion position and the maximum dorsiflexion position of the ankle joint. A comparison was made between different groups at the same position in the value of SWV. Meanwhile, the test-retest reliability of SWV was also analyzed. Results: In natural position of the ankle joint, SWV value of MG obtained in Group One was higher than Group Two and the control group (P<0.05); SWV value of SOL obtained in Group One was higher than the control group (P<0.05). In maximum metatarsal flexion position of the ankle joint, there was no significant difference in SWV value of each muscle among the three groups (P>0.05). In maximum dorsiflexion position of the ankle joint, SWV value of MG obtained in Group Two was higher than the control group (P<0.05), while all other differences between the groups were not statistically significant (P>0.05). The SWV of MG and the SWV of LG both had excellent test-retest reliability as intraclass correlation coefficient (ICC) calculation of three different positions were all larger than 0.75. And ICC calculation of the SWV of SOL at three different positions were between 0.45-0.75. Conclusion: ARFI can be used to quantitatively evaluate the elastic changes of triceps surae with venous plexus dilatation, which may become an important technique to further study the intrinsic relationship between the muscle elasticity and the triceps surae venous plexus dilatation disease. 2020 Vol. 50 (2): 115-119 [Abstract] ( 731 ) HTML (1 KB)  PDF (1374 KB)  ( 724 ) 120 The influence of miR-144 on cell proliferation and cell cycle of papillary thyroid carcinoma cells YU Mingjun, ZHAO Na, WANG Haiming DOI: 10.3969/j.issn.2095-9400.2020.02.007 Objective: To evaluate the expression levels of miR-144 in papillary thyroid carcinoma (PTC) cell lines. To explore the influence of up-regulation/down-regulation of miR-144 on cell proliferation and cell cycle in PTC cells. Methods: The expression levels of miR-144 in PTC cells K1, TPC-1 and human thyroid cells Nthy-ori 3-1 were detected by qPCR. K1 cells were transiently transfected with miR-144 mimics, inhibitor, or the negative control (NC) by Lipo2000. After transfection, MTT assay, colony formation assay and flow cytometry were conducted to explore the impact of miR-144 on cell proliferation ability and cell cycle of K1 cells. Western blot was provided to detect the protein expression of Cyclin D1. Results: Compared with thyroid cells, the relative expression levels of miR-144 in PTC cells were significantly decreased (P<0.05). The expression of miR-144 in K1 cells was lowest. The overexpression of miR-144 suppressed K1 cell proliferation and colony formation, caused K1 cells arrested in G0/G1 phase, which relate to downregulation of Cyclin D1. Whereas, low expression of miR-144 promoted cell proliferation, colony formation and accelerate the process of cell cycle.Conclusion: miR-144 was down-regulated in PTC cells. miR-144 may act as a tumor suppressor in PTC by inhibiting cell proliferation and blocking cell cycle, suggesting its use as a potential therapeutic target.  2020 Vol. 50 (2): 120-124 [Abstract] ( 835 ) HTML (1 KB)  PDF (1529 KB)  ( 965 ) 125 Correlation between changes of plasma cortisol concentration and symptom improvement in patients with post schizophrenia depression Liu Qiaojin, Wen Na, Zhu Cheng, Lu Fanfan DOI: 10.3969/j.issn.2095-9400.2020.02.008 Objective: To explore the relationship between the changes of plasma cortisol levels and the improvement in patients with post schizophrenia depression. Methods: A total of 60 post schizophrenic depression patients from the Affiliated Kangning Hospital of Wenzhou Medical University between March 2018 and December 2018 were enrolled as case group, while 60 cases of schizophrenia after recovery were enrolled as the control group. Both the case group and the control group were only previously treated with risperidone. The plasma cortisol level of the patients was measured by ELISA after entering the group. Paroxetine was added to the case group. Four weeks before and after the treatment, the depressive symptoms of the case group were evaluated by the HAMD scale. After 4-week treatment, the plasma cortisol level of the case group was measured by ELISA. Results: The plasma cortisol level of cases group was significantly higher than that of the control group (t=7.206, P<0.001). After 4 weeks’ treatment, the HAMD total score and factor score of anxiety/somatization, body weight, cognitive disorder, day and night change, retardation, sleep disorder, hopelessness and the plasma cortisol level of the case group were significantly lower than those before treatment (P<0.001). Before and after treatment, the changes of plasma cortisol of the case group were positively related to the improvement of HAMD total scores and factor score of anxiety/somatization, cognitive and hopelessness (r=0.346, 0.367, 0.297, 0.246, P<0.05). The results of linear regression analysis showed the changes of cortisol in the case group could explain 22.5% of the HAMD total, 26.5% of the anxiety/somatization, 17.6% of the cognitive, 13.6% of the hopelessness respectively. Conclusion: The changes of plasma cortisol levels were significantly related to the improvements of symptoms in patients with post schizophrenia depression, so the abnormal plasma cortisol levels might be one of the factors influencing the incidence of post schizophrenia depression. 2020 Vol. 50 (2): 125-129 [Abstract] ( 1012 ) HTML (1 KB)  PDF (1378 KB)  ( 1069 ) 130 Genetic analysis of the pedigree with inherited coagulation factorXII deficiency caused by compound heterozygous mutations LI Shaoxi, LI Xiaolong, JIN Yanhui, YANG Lihong, ZHANG Haiyue, WANG Mingshan DOI: 10.3969/j.issn.2095-9400.2020.02.009 Objective: To detect the laboratory phenotype and genotype of a hereditary factorXII (FXII) deficient pedigree caused by compound heterozygous mutations, which will help us to investigate the causative gene and its molecular pathogenesis. Methods: Prothrombin time (PT), activated partial thromboplastin time (APTT), coagulation factor VIII activity (FVIII:C), coagulation factor IX activity (FIX:C), coagulation factor XI activity (FXI:C), FXII activity (FXII:C), and FXII antigen (FXII:Ag) were assayed in proband and his family members (five persons in three generations) to confirm the diagnosis. All exons and flanking sequences of the F12 gene of the proband were analyzed by direct PCR sequencing. The mutation sites were confirmed by reverse sequencing and the corresponding mutation site regions of the family members were detected. The conservation of amino acid mutation sites were analyzed using ClustalX-2.1-win software, the effect of mutations on protein functions were analyzed using four online bioinformatic tools (Mutation Taster, Poly-Phen-2, PROVEAN and SIFT); the mutation sites were analyzed by Swiss-Pdb Viewer 4.0.1 for molecular structure and protein interaction. Results: The APTT of the proband is 59.1 s, prolonged obviously, and the FXII:C decreased to 4%, the FXII:Ag decreased to 5%; the FXII:C and the FXII:Ag of his father, mother and daughter decreased to 32%-37%. Genetic analysis revealed that there were two compound heterozygous mutations in exon 13 of the F12 gene in the proband: one was the c.1561G>A point heterozygous missense mutation (p.Glu502Lys); another was the c.1637T>C point heterozygous missene mutation (p.Met527Thr). His father was carried the p.Met527Thr mutation, his mother and daughter were carried the p.Glu502Lys mutation. Conservative analysis showed that Glu502 and Met527 were highly conserved among homologous species. The four online bioinformatics sofwares predicted results for these mutations were consistent: all predicted that these mutations were likely to be deleterious mutations that could cause related diseases. Mutant protein model analysis showed that wild-type Glu502 forms a hydrogen bond with His365, the mutant Lys502 resulted in the disappearance of the hydrogen bond between Lys502-His365. Replacement of Met527 with Thr527 resulted in the addition of two hydrogen bonds between Thr527-Leu527. Conclusion: There were two compound heterozygous mutations p.Glu502Lys and p.Met527Thr in exon 13 of the F12 gene in the proband, which were speculated that the heterozygous mutations were inherited from his parents with the heterozygotes of p.Glu502Lys and p.Met527Thr respectively and were associated with the reduced blood FXII levels in the family. 2020 Vol. 50 (2): 130-134 [Abstract] ( 979 ) HTML (1 KB)  PDF (1570 KB)  ( 1148 ) 135 Clinical significance of muscle visceral ratio in patients with type 2 diabetes complicated with nonalcoholic fatty liver ZHANG Fan, SU Xiaoyou, XU Jing, ZHENG Chao DOI: 10.3969/j.issn.2095-9400.2020.02.010 Objective: To evaluate the muscle fat ratio in patients with type 2 diabetes mellitus complicated with non-alcoholic fatty liver disease (NAFLD). Methods: A total of 394 patients with type 2 diabetes mellitus from the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from April to August 2017 were enrolled in this study. The patients were divided into two groups: complicated NAFLD group (n=159) and uncomplicated NAFLD group (n=235). The clinical date such as skeletal muscle mass, visceral fat area, blood glucose, blood lipid were compared between the two groups. Pearson correlation analysis was for analyzing the relationship between muscle fat ratio and metabolic risk factors, multivariate logistic regression for the effect of muscle fat ratio on the development of NAFLD, and ROC curve for the predictive value of muscle fat ratio for NAFLD. Results: Compared with uncomplicated NAFLD group, body mass, body mass index, waist circumference, visceral fat area, body fat percentage, diastolic blood pressure, blood uric acid, glycated hemoglobin level, TC and TG were significantly higher in complicated NAFLD group, while muscle fat ratio and limb skeletal muscle mass lower (P<0.05). Pearson correlation analysis revealed a negative correlation between muscle fat ratio and body mass index, waist circumference, and systolic blood pressure in the male group (r= -0.365, -0.437, -0.117, P<0.01), and a negative correlation between muscle fat ratio and body mass index and waist circumference in the female group (r=-0.190, -0.212, P<0.01). Multivariate analysis revealed that muscle fat ratio was an independent risk factor for NAFLD in female with type 2 diabetes (OR=4.21, 95%CI=1.31-13.52, P=0.016). ROC curve analysis showed that the area under AUC curve of male group and female group was 0.66 and 0.63 respectively. Conclusion: The level of muscle fat ratio is closely related to the occurrence of NAFLD in type 2 diabetic patients, which can serve as an important reference index for clinical evaluation of type 2 diabetic patients complicated with NAFLD. 2020 Vol. 50 (2): 135-140 [Abstract] ( 754 ) HTML (1 KB)  PDF (1436 KB)  ( 870 ) 141 An imaging analysize of growing pain in children’s lower limbs LIN Xubo, FU Yao, WU Aiqin, YANG Yubing, ZHENG Wenlong DOI: 10.3969/j.issn.2095-9400.2020.02.011 Objective: To investigate the imaging manifestations of growing pain in the lower limbs of children and to better understand this disease. Methods: The imaging findings of 4 patients with growing pain in the lower limbs in children were retrospectively studied. Results: X-ray manifestations showed no abnormalities in both knees and middle-lower segments of tibiofibula. MRI manifestations of knee joint included symmetrical patchy, spotted T1WI low signal and FS-T2WI/PD high signal shadows were found in both sides of medullary cavity of femur, tibia metaphysis and epiphyseal. The cortex was intact and continuous, and no obvious abnormal signal was found in the soft tissue around the joint. The abnormal changes of both tibia and soft tissue of leg were symmetrical patches, dotted T1WI low signal and FS-T2WI high signal shadows with unclear boundary, which were found in the middle and lower tibial medullary cavity on both sides with intact cortex. The medial head of gastrocnemius and anteromedial fascia of tibia showed strip-like low signal on T1WI and high signal on FS-T2WI. The AV values of the transverse and sagittal positions from the two groups were statistically different (P=0.001). Conclusion: The imaging changes of growing pain in children’s lower limbs are characterized by normal X-ray film and symmetrical bilateral bone marrow edema on MR. Combined with clinical signs, certain imaging diagnosis can be made. 2020 Vol. 50 (2): 141-144 [Abstract] ( 940 ) HTML (1 KB)  PDF (1504 KB)  ( 1028 ) 145 The application of RNA simultaneous amplification and testing in the detection of male gonococcal urethritis DAI Xianning, TONG Yu, LIN Jianping, Shi Jianyou, Chen Lu, XU Kai DOI: 10.3969/j.issn.2095-9400.2020.02.012 Objective: To evaluate the application value of RNA simultaneous amplification and testing technology in the detection of male gonococcal urethritis. Methods: From June 2017 to August 2018, a total of 322 male patients with suspected gonococcal urethritis infection in Wenzhou People’s Hospital were detected by RNA simultaneous amplification and testing (RNA-SAT) and real-time fluorescence quantitative PCR (FQ-PCR), then the results were statistically analyzed. Results: The highest NG positive rate was 35.1% (113/322) by urine RNA-SAT method, and the lowest NG positive rate was 26.1% (84/322) by urine FQ-PCR method. The difference of positive rates in urine RNA-SAT, urine FQ-PCR and isolation culture was statistically significant (χ2=6.312, P<0.05). The difference of positive rates in swab RNA-SAT, swab FQ-PCR and isolation culture was not statistically significant (χ2=1.039, P>0.05). The highest sensitivity of urine RNA-SAT was 97.9% (93/95), and that of urine FQ-PCR method was 81.1% (77/95). According to ROC curve, swab RNA-SAT and urine RNA-SAT method have a relatively high diagnostic value (AUC>0.9). After 7 days of treatment, clinical symptoms in 81 patients disappeared and negative leukocyte were re-examined. The difference in negative rates in urine RNA-SAT, urine FQ-PCR and isolation culture was not statistically significant (χ2=2.531, P>0.05). The difference in negative rates of swab RNA-SAT, swab FQ-PCR and isolation culture was also not statistically significant (χ2=2.933, P>0.05). Conclusion: Both RNA-SAT and FQ-PCR can be used to diagnose NG infection; however, RNA-SAT has the advantages of convenient sampling, short time, little pollution and accurate results. So it can be used to detect NG in male gonococcal urethritis and to evaluate the effect and prognosis. 2020 Vol. 50 (2): 145-148，153 [Abstract] ( 961 ) HTML (1 KB)  PDF (1398 KB)  ( 1110 ) 149 Active and passive surveillance on post-marketing safety of enterovirus 71 inactivated vaccine CHEN Lingping, SUN Xiaoyu, WEI Jingjiao, ZHENG Xiaochun, LI Wancang, WANG Zhigang DOI: 10.3969/j.issn.2095-9400.2020.02.013 Objective: To evaluate post-marketing safety of enterovirus 71 inactivated vaccine (EV71 vaccine), and provide scientific basis for formulating immunization strategy. Methods: The adverse reactions of EV71 vaccine in Wenzhou city were collected by a combination of active and passive surveillance methods and analyzed statistically by SPSS23.0 software. Results: The incidence of adverse reactions of EV71 vaccine was 4.2/10 000 doses in passive surveillance, of which the incidence of abnormal reactions was 0.5/10 000 doses. The incidence of adverse reactions was 156.7/10 000 doses in active surveillance, of which the incidence of abnormal reactions was 12.0/10 000 doses. The incidence of adverse reactions and abnormal reactions in active surveillance were both higher than those in passive surveillance, with statistical difference (P<0.05). Active surveillance showed that adverse reactions mainly occurred within 30 min-3 d (62.4%) after immunization, and the main clinical symptom was fever. With the increase in age, the incidence of adverse reactions showed a downward trend (P<0.05). There was a significant difference in the rate of adverse reactions between different genders, i.e. higher in women than in men (P<0.05). Conclusion: The incidence of adverse reactions of EV71 vaccine was low, the symptoms were common and mild, and the overall safety was good. Active surveillance was more sensitive and accurate than passive surveillance. 2020 Vol. 50 (2): 149-153 [Abstract] ( 843 ) HTML (1 KB)  PDF (1361 KB)  ( 837 ) 154 CT findings of thymic cysts with different densities and their pathological basis QIU Liyan, JIN Zhonggao, JIA Xiupeng, HU Bibo, JIN Yinhua, ZHENG Jianjun DOI: 10.3969/j.issn.2095-9400.2020.02.014 Objective: To explore the CT manifestations of thymic cysts with different densities and their pathological basis. Methods: The CT findings and pathological changes of 45 cases of thymic cysts with solid density and 23 thymic cysts with liquid density confirmed by operation and pathology were retrospectively analyzed. Results: There was a correlation between the density of thymic cysts and the distribution of size (P<0.01). The shape of the solid density cysts was regular, while the liquid density cyst was irregularly shaped along the large vessel gap. There was a significant difference between different groups (P<0.01). However, tear droplets and triangles were found in both groups without significant difference in the distribution between the two groups (P>0.05). Compared with the liquid density cysts, the solid density cysts were mostly not herniated outside the thymus profile, and the difference was statistically significant (P<0.01). The pathologic features of solid density thymic cysts included pseudostratified ciliated columnar epithelium or ciliated columnar epithelium with secretory function, dense arrangement of the epithelial cells of the cyst wall, and turbid cystic fluid. The surface of the liquid density thymic cysts was composed mainly of non-secretory flattened or columnar epithelium, sparse or even absent, the weak base layer and usually clear cystic fluid. There was significant difference between cyst density and epithelial type (P<0.05). Conclusion: The imaging findings of the solid density thymic cysts were different from liquid density thymic cysts. Their pathologic basis was helpful to understand the different manifestations of thymic cysts. 2020 Vol. 50 (2): 154-157 [Abstract] ( 870 ) HTML (1 KB)  PDF (1425 KB)  ( 1059 ) 158 The effect of Ghrelin on gastric emptying and lower esophageal sphincter pressure in rats and its mechanism CAO Shuguang, WU Hao, LIN Limiao DOI: 10.3969/j.issn.2095-9400.2020.02.015 Objective: To investigate the effects of Ghrelin on gastric emptying and lower esophageal sphincter pressure (LESP), expressions of Motilin and Substance P (SP) in plasma, and to explore the mechanism of Ghrelin on gastric emptying and LESP. Methods: After Wistar rats were intraperitioneally injected with different doses of Ghrelin (0.5, 1.0, 1.5, 2.0, 2.5 nmol/kg), gastric emptying rate was measured; after intraperitoneal injecion of 1.5 nmol/kg, LESP was measured, and plasma Motilin and SP concentrations were determined by radioimmunoassay. Results: After intraperitoneal injection of Ghrelin, gastric emptying rate was significantly faster than controls [(38.24±7.15)% vs. (27.03±2.91)%], and the accelerated gastric emptying had a significant dose-response relationship with Ghrelin. After intraperitoneal injection of Ghrelin, LESP [(19.57±3.26) mmHg] in rats was significantly increased compared with the control group [(13.78±2.29)mmHg] (P<0.05), suggested that Ghrelin could increase the LESP in rats. After intraperitoneal injection of Ghrelin, plasma Motilin concentration [(217.28±25.38)ng/L] in rats was significantly increased compared with the control group [(190.02±18.18)ng/L] (P<0.05), suggesting that Motilin may be involved in the LESP regulation in rats. Conclusion: Intraperitoneal injection of Ghrelin can accelerate gastric emptying in rats, Ghrelin may increase the pressure of the lower esophageal sphincter in rats, and Motilin may be involved in the LESP regulation. 2020 Vol. 50 (2): 158-161 [Abstract] ( 634 ) HTML (1 KB)  PDF (1341 KB)  ( 838 ) 162 The effect of optimized diet intervention on secondary hyperparathyroidism in patients with maintenance hemodialysis WANG Yan, WANG Deguang, WANG Juxia DOI: 10.3969/j.issn.2095-9400.2020.02.016 Objective: To observe the effect of optimized dietary intervention on secondary hyperparathyroidism (SHPT) in patients with maintenance hemodialysis (MHD). Methods: Seventy patients undergoing MHD and SHPT were divided into two groups according to their admission time, with 35 cases in each group. The control group was treated with Alfacalcicol soft capsules, calcium carbonate tablets and other dietary treatments, while an optimized diet intervention was implemented in the intervention group, based on the routine treatment. The experiment lasted for three months. Serum calcium, phosphorus, and whole-parathyroid hormone (iPTH) levels were measured before and after intervention in both groups, and the calcium-phosphorus product was calculated. Meanwhile, every result was given a certain score respectively by using the SF-36 scale. The score refers to the score of all items added in the comprehensive questionnaire. Results: The blood calcium levels were increased after intervention in both groups (P<0.05). The levels of phosphorus, iPTH and calcium and phosphorus were lower (P<0.05), and the decline degree of serum phosphorus, iPTH and calcium and phosphorus product were higher in the intervention group than in the control group (P<0.05). The scores, factors and total scores of the two comprehensive measurement items of SF-36 in intervention group were significantly higher than those in the control group (P<0.01). Conclusion: Optimized dietary intervention can significantly improve serum calcium and phosphorus levels in patients with MHD and SHPT, and effectively improve the scores of SF-36. 2020 Vol. 50 (2): 162-165 [Abstract] ( 799 ) HTML (1 KB)  PDF (1368 KB)  ( 681 ) 166 The application of progressive focusing interview in the rehabilitation of schizophrenic patients LIN Chunyan, LIN Cuilu, ZHOU Xuechou, CHEN Yalin DOI: 10.3969/j.issn.2095-9400.2020.02.017 Objective: To explore the application effect of progressive focusing interview in the rehabilitation of schizophrenia patients. Methods: Eighty four cases of schizophrenic patients admitted to the Seventh People’s  Hospital of Wenzhou from October 2017 to October 2018 were selected and randomly divided into the observation group and the control group with 42 cases each. The control group received routine nursing, the observation group was intervened with progressive focusing interview. Mental symptom severity, social function and relapse were compared before and after the intervention. Results: After the intervention, the brief psychiatric rating scale (BPRS) score and social disability screening schedule (SDSS) score in the two groups were significantly lower than that before the intervention (P<0.05), and the observation group was significantly lower than the control group (P<0.05). Three months later, the recurrence rate of the control group was 35.71% (15/42) and that of the observation group was 11.90% (5/42). There was a significant difference in the recurrence rate of mental symptoms between the two groups (P<0.05). Conclusion: Well applied in patients with schizophrenia, the mode of progressive focused interview is can play a significant role in reducing the severity and recurrence rate of mental symptoms in schizophrenia patients and rehabiliating their social functions. 2020 Vol. 50 (2): 166-169 [Abstract] ( 810 ) HTML (1 KB)  PDF (1371 KB)  ( 991 )

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