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2020 Vol. 50, No. 1 Published: 2020-01-25   1 Differential analysis of gene expression profiles of nasopharyngeal carcinoma based on a combined analysis of multiple GEO chip databases WANG Xiaoqiong, JIN Qiaozhi, Chen Wubing, Cai Zhiyi DOI: 10.3969/j.issn.2095-9400.2020.01.001 Objective: To screen the key genes of nasopharyngeal carcinoma (NPC) and to explore the pathogenesis of nasopharyngeal carcinoma. Methods: The differentially expressed genes (DEGs) in NPC were identified by Gene Expression Omnibus (GEO) combined with R language. Gene ontology (GO) database and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used for enrichment and functional annotation of DEGs. A protein-protein interaction (PPI) network was constructed based on STRING database and the key gene clusters and network nodes of NPC were further analyzed by Cytoscape software. Results: A total of 3 sets of nasopharyngeal cancer gene chip data were included. 116 differentially expressed genes with intersection in this 3 data sets were selected, including 69 up-regulated genes and 47 down-regulated genes. GO enrichment analysis of differentially expressed genes revealed abnormal cell division and spindle assembly, changes in protein biding and in extracellular body function, which were associated with the occurrence and development of nasopharyngeal carcinoma. Some intracellular signaling pathways of nasopharyngeal carcinoma were significantly altered, including DNA repair, extracellular matrix receptor interaction, cell cycle small cell lung cancer, human T-cell leukemia virus 1 infection, etc. The protein interaction network suggests that there are three important gene clusters and four key genes. Conclusion: Bioinformatics can effectively screen target genes and signal pathways, which provides new ideas for the treatment of nasopharyngeal carcinoma. 2020 Vol. 50 (1): 1-6,11 [Abstract] ( 1032 ) HTML (1 KB)  PDF (2031 KB)  ( 952 ) 7 miRNA let-7a inhibits ACVR1B expression HONG Zipu, GUAN Kaiyu, WANG Shao, XU Shengnan, HAO Yanyan, CHEN Xiaoming DOI: 10.3969/j.issn.2095-9400.2020.01.002 Objective: To investigate the effect of miRNA let-7a on ACVR1B expression. Methods: Bioinformatics analysis predicted that activin receptor type 1B (ACVR1B) is a potential target gene for let-7a. By constructing ACVR1B 3’UTR wild-type and ACVR1B 3’UTR mutant dual-luciferase reporter vector (pmirGLO-ACVR1B 3’UTR-WT and pmirGLO-ACVR1B 3’UTR-MUT), let-7a mimic or let-7a negative control (let-7a NC) were co-transfected into HEK293T cells respectively and the luciferase activity of each group was detected by dual-luciferase reporter system after the cells were collected. qRT-PCR and Western blot were used respectively to detect the ACVR1B mRNA and protein levels after transfection of let-7a mimics, let-7a inhibitor and let-7a NC respectively. Results: The bioinformatics analysis revealed that the human ACVR1B mRNA 3’UTR region (position 77-83) contains perfect complementarity region with a seed sequence of let-7a. The luciferase activity of the let-7a mimic and pmirGLO-ACVR1B 3’UTR-WT co-transfected group was significantly lower than the let-7a NC and pmirGLO-ACVR1B 3’UTR-WT co-transfected group (P<0.05), while the luciferase activities of pmirGLO-ACVR1B 3’UTR-MUT transfected groups were no statistical significance (P>0.05). In HEK293T cells, mRNA and protein levels of ACVR1B in let-7a mimic transfected group were significantly lower than let-7a NC group and let-7a inhibitor group (P<0.05), and the expression levels decreased by 70% and 50% respectively. Conclusion: ACVR1B was a potential target gene directly regulated by let-7a and let-7a might suppress the expression of ACVR1B gene in HEK293T cells. 2020 Vol. 50 (1): 7-11 [Abstract] ( 820 ) HTML (1 KB)  PDF (1673 KB)  ( 903 ) 12 The underlying mechanism of miR-148b in regulating chemosensitivity of diffuse large B cell lymphoma LIN Xiaoji, FANG Weiyue, DONG Yuqing, SUN Ni, SU Tong, YANG Xiangchou, GUO Wenjian, HE Muqing, YAO Rongxin DOI: 10.3969/j.issn.2095-9400.2020.01.003 Objective: To explore the functional role of miR-148b in regulating chemosensitivity in diffuse large B-cell lymphoma (DLBCL) by targeting Ezrin protein. Methods: After the human DLBCL resistant cell line CRL2631/CHOP was induced, the expression of miR-148b in CRL2631 cells and CRL2631/CHOP cells was observed by real-time fluorescent quantitative PCR and the difference of Ezrin protein expression between the two groups was measured by Western blot. Double luciferase test was performed to detect the targeted binding between miR-148b and Ezrin. Sensitivity of CRL2631/CHOP cells and CRL2631 cells to CHOP was determined by microRNAs simulation/interference technique and CCK-8 method. Results: The expression level of miR-148b in CRL2631/CHOP cells was significantly lower than that in CRL2631 cells (P=0.001). The expression level of Ezrin protein in CRL2631/CHOP cells was obviously higher than that in CRL2631 cells (P<0.05). The results of the double luciferase assay indicated that Ezrin was a direct target gene of miR-148b. The expression of Ezrin and MDR-1 protein in CRL2631/CHOP cells transfected with miR-148b mimic were down regulated (P<0.05) and the sensitivity of transfected CRL2631/CHOP cells to CHOP was strengthened (P<0.05). Furthermore, the expression of Ezrin and MDR-1 protein transfected with miR-148b inhibitor in CRL2631 cells were up-regulated with the sensitivity to CHOP diminished (P<0.05). Conclusion: miR-148b regulates the chemosensitivity of DLBCL cells to CHOP by targeting Ezrin. 2020 Vol. 50 (1): 12-18 [Abstract] ( 819 ) HTML (1 KB)  PDF (2038 KB)  ( 773 ) 19 The expression of miR-144-5p, miR-668-3p and miR-134-5p in children with primary immune thrombocytopenia JIN Susu, LI Fanfan, YE Manli, SHU Kuangyi, Li Shanshan, LIU Jie, JIANG Minghua DOI: 10.3969/j.issn.2095-9400.2020.01.004 Objective: To investigate the changes of microRNAs (miR-144-5p, miR-668-3p and miR-134-5p) in peripheral blood mononuclear cells (PBMCs) in children with primary immune thrombocytopenia (ITP). Methods: Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) was used to detect the relative expression of the three miRNAs from 25 ITP patients and healthy control in PBMCs. Platelet count and immature platelet fraction (IPF%) were detected by Sysmex XE-5000 automatic blood analyzer. The correlations of the miRNAs expression with peripheral platelet count were analyzed and diagnostic value was measured by receiver operating characteristic curve. Results: Compared with the normal control group, the expressions of miR-144-5p and miR-668-3p of the ITP group in PBMCs were significantly up-regulated (P<0.05), and were negatively correlated with platelet count (r=-0.802, P<0.001; r=-0.753, P<0.001), while the expressions of miR-134-5p was down-regulated significantly (P<0.05). The expressions of miR-144-5p and miR-668-3p in the acute ITP group were higher than those in the chronic ITP group (P<0.05). In terms of the single indicator, the AUC of miR-144-5p combined with IPF% in the diagnosis of acute ITP was 0.960. The sensitivity and specificity were 93.3% and 100% respectively. The AUC of miR-134-5p combined with IPF% in the diagnosis of chronic ITP was 0.967. The sensitivity and specificity were 90.0% and 93.3% respectively. Conclusion: miR-144-5p and miR-668-3p in PBMCs were significantly up-regulated and miR-134-5p was down-regulated in children with ITP, which suggests they may play an important role in the incidence and development of ITP. 2020 Vol. 50 (1): 19-23 [Abstract] ( 719 ) HTML (1 KB)  PDF (1658 KB)  ( 655 ) 24 The protective effect of Celastrol on H9C2 cell injury induced by high glucose WU Zhang, LYU Wang, CHEN Xinguo, JIN Shuang, CHEN Linglong, LU Zhongqiu DOI: 10.3969/j.issn.2095-9400.2020.01.005 Objective: To simulate in vitro the cellular model of DCM by high glucose culture medium of rat cardiomyocytes H9C2 and to study the protective effect and mechanism of Celastrol on H9C2 cells. Methods: In the experiment, low glucose group (5.6 mmol/L), isoosmotic group (mannitol isoosmotic culture), high glucose treatment group (30 mmol/L) and Celastrol group (high glucose culture and intervention with 10 ng/mL and 100 ng/mL Celastrol) were established. MTT assays were used to analyze the viability of H9C2 cells in high glucose treatment group and Celastrol group. Reactive oxygen species cluster fluorescence probe staining and flow cytometry were performed to analyze the production of ROS in H9C2 cells induced by high glucose culture and intervened with Celastrol. The mRNA and protein expression level of NF-κB, TGF-1β and IL-1β were detected by RT-PCR and Western blot. Results: Significant inhibitory effects on the viability of H9C2 cells were observed in high glucose treatment group, with statistically significant difference compared with the low glucose group (P<0.05). The proliferation inhibitory effect was attenuated when intervened with different concentrations of Celastrol. The ROS levels in high glucose treatment group was significantly higher than the low glucose group (P<0.05), and the ROS levels were decreased after intervention of 100 ng/mL Celastrol. RT-PCR, Western blot results showed that the mRNA and protein expression levels of NF-κB, TGF-1β and IL-1β genes were up-regulated in high glucose treatment group, compared with low glucose and the isotonic group, the differences were statistically significant (P<0.05). The mRNA and protein expression levels of NF-κB, TGF-1β and IL-1β in 10 ng/mL and 100 ng/mL Celastrol group were down-regulated compared with high glucose treatment group, the differences being significant (P<0.05). Conclusion: Celastrol could inhibit production of ROS in H9C2 cells in high glucose culture and reduce the growth inhibition effect on cells, thus producting protective effects on cardiac myocytes. The mechanism may be related to the inhibited expression of inflammatory factor-related genes. 2020 Vol. 50 (1): 24-29,35 [Abstract] ( 861 ) HTML (1 KB)  PDF (2050 KB)  ( 790 ) 30 The association of CD4+, CD8+ T lymphocytes in colorectal cancer with postoperative prognosis FU Chao, MEI Tingting, WANG Hongtao, TAO Yong, WANG Dong, QIU Jianming, YANG Guangen DOI: 10.3969/j.issn.2095-9400.2020.01.006 Objective: To investigate the association of CD4+, CD8+ T lymphocytes in colorectal cancer with postoperative prognosis. Methods: Clinical data and resection samples of 109 colorectal cancer patients identified by pathology undergoing resection in our department from January 2012 to January 2014 were collected. The CD4+, CD8+ T lymphocytes in colorectal cancer were detected by immunohistochemical staining and grouped according to the high and low levels. Kaplan-Meier survival curve analysis was used to compare clinical features and prognosis. The Cox proportional hazard regression model was used to analyze the clinical factors that may affect the prognosis of patients with univariate and multivariate analysis. Results: Immunohistochemical staining showed that CD8+ T cell infiltration was high in 78 cases, low in 31 cases; CD4+ T cell infiltration was high in 56 cases, and low level in 53 cases. The levels of CD4+ and CD8+ T lymphocytes in colorectal cancer tissues were closely related to tumor stage and depth of invasion (all P<0.05). Follow-up analysis showed that colorectal cancer patients with high levels of CD8+ and their CD8+/CD4+ had a better prognosis for DFS and OS (P<0.05). And there was no significant difference between DFS, OS and low-level group in patients with high-level CD4+ colorectal cancer. Cox regression model analysis showed that tumor stage, lymph node metastasis, CD8+ and CD8+/CD4+ were independent risk factors for DFS and OS prognosis in patients with colorectal cancer (P<0.05). Conclusion: The level of CD8+ and CD8+/CD4+ could be a good reference value for the prognosis of colorectal cancer patients. 2020 Vol. 50 (1): 30-35 [Abstract] ( 1151 ) HTML (1 KB)  PDF (1663 KB)  ( 890 ) 36 The third-generation dual-source CT+flash mode scan combined with chest circumference index to reduce the radiation dose and iodine intake of coronary CTA WANG Hailin, CHEN Minjiang, WENG Qiaoyou, LU Chenying, CHENG Shufang, YE Miaoqing, LIN Guihan, JI Jiansong DOI: 10.3969/j.issn.2095-9400.2020.01.007 Objective: To explore the clinical application value of third-generation dual-source CT+flash mode scan combined with chest circumference index to reduce the radiation dose and iodine intake of coronary artery CT angiography (CTA). Methods: A total of 160 patients with clinically suspected coronary heart disease requiring coronary CTA examination were continuously selected and examined by trube-flash large pitch scan combined with iterative reconstruction technology. Tube current automatic adjustment technology care Dose 4D was operated. The control group included 60 cases, with tube voltage=120 kV, and the remaining 100 cases were in the low-dose group, which were sub-divided into groups A, B, and C according to the parameters of the chest circumference. Among them, the chest circumference of group A was≤85 cm, the tube voltage was 70 kV; group B was 85-90 cm, tube voltage=80 kV; group C was≥95 cm, tube voltage=90 kV. One-way analysis of variance was used to compare gender, age, BMI, chest circumference, tube voltage, tube current, right coronary CT value, SD value, SNR, CTD, DLP, radiation dose in four groups of patients; the comparison of subjective image quality score was performed by a nonparametric rank sum test. Results: The coronary image quality scores of the conventional group and the low-dose group (group A, group B, and group C) were 3.26±0.23, 3.18±0.19, 3.23±0.31, and 3.21±0.25, respectively. There was no significant difference between the groups (P>0.05), and the image quality met the requirements of clinical diagnosis. There were no significant differences in age, gender and heart rate between the 4 groups (P>0.05). The CT values of the four groups of right coronary artery were (425.94±69.59)Hu, (505.23±41.41) Hu, (463.83±59.68) Hu and (444.70±70.36) Hu, respectively, and there was statistical difference among the low-dose groups and the conventional group (P<0.01). The radiation doses were (1.03±0.15) mSv, (0.36±0.09) mSv, (0.57±0.05) mSv and (0.80±0.10) mSv (P>0.05); the iodine intakes were 18.50 g, 9.45 g, 12.80 g, and 14.40 g, respectively. The radiation dose and iodine intake of the low-dose groups were lower than the conventional group (P<0.01). Conclusion: The third-generation dual-source CT+flash mode scanning combined with chest circumference index can be applied to adjust the tube voltage and meanwhile the contrast agent with low iodine concentration can be taken to adjust the rate and total amount of contrast agent, thus effectively reducing the radiation dose and iodine intake while the image quality is ensured. 2020 Vol. 50 (1): 36-40 [Abstract] ( 790 ) HTML (1 KB)  PDF (1616 KB)  ( 757 ) 40 Anti-anxiety effects and mechanism of omega-3 fatty acids in ovariectomized anxiety-like mice HE Chujun, TU Junxue, HUANG Wanran, BAO Shihui DOI: 10.3969/j.issn.2095-9400.2020.01.009 Objective: To analyze the anti-anxiety effect of omega-3 polyunsaturated fatty acids (n-3 PUFA) in ovariectomized mice subject to unpredictable chronic stress and to reveal its mechanism. Methods: Chronic unpredictable stress was performed 1 week after bilateral ovarian ablation in mice to establish the model of menopausal anxiety. Using classical anxiety behavior tests (e marble-burying test and elevated plus maze test) to verify the anti-anxiety effect of chronic administration of n-3 PUFA (10, 20, 60 mg/kg) for 14 days. The mechanism of antianxiety of n-3 PUFA was investigated by HPLC method to detect the contents of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), noradrenaline (NE), dopamine (DA), dopac neurotransmitters and their metabolites. Results: Chronic administration of menopausal anxiety-like mice with n-3 PUFA (20, 60 mg/kg) effectively decreased the number of buried marbles and elevated the number and time to open arm at elevated plus maze test (P<0.05). HPLC results showed that chronic administration of menopausal anxiety-like mice with n-3 PUFA (20, 60 mg/kg) significantly affected 5-HT, 5-HIAA/5-HT, NE, DA in prefrontal cortex (P<0.05) and also significantly improved the concentration of 5-HT, 5-HIAA/5-HT, NE in hippocampus (P<0.05) and regulated the level of 5-HT, NE in hypothalamus (P<0.05). In summary, the possible mechanism of n-3 PUFA involves regulating neurotransmitters and its metabolite pathways in hippocampus, prefrontal cortex and hypothalamus. Conclusion: n-3 PUFA is an ideal drug for treating menopausal anxiety through regulating the neurotransmitters and its metabolites in hippocampus, prefrontal cortex and hypothalamic. 2020 Vol. 50 (1): 40-50,55 [Abstract] ( 801 ) HTML (1 KB)  PDF (1803 KB)  ( 815 ) 41 Expression of PD-L1 and its clinical significance in colorectal cancer DU Zhou, HUANG Ping, HUANG Guoyu, HAN Shaoliang, ZUO Zhigui DOI: 10.3969/j.issn.2095-9400.2020.01.008 Objective: To investigate the expression of PD-L1 in colorectal cancer and its relationship with clinicopathologic features, and meanwhile to discuss the clinical significance of PD-L1 expression. Methods:Colorectal cancer tissues and adjacent normal tissues were obtained from 50 patients with colorectal cancer. RTPCR and Western blot were conducted to detect the expression of PD-L1 in mRNA and protein levels. Results:The expression of PD-L1 in colorectal cancer tissues was elevated, compared with that in adjacent normal tissues (P<0.05). The elevated expression of PD-L1 in colorectal cancer tissues was correlated with cancer differentiation degree and lymphatic metastasis (P<0.05). Conclusion: PD-L1 was highly expressed in colorectal cancer patients, which was correlated with the clinicopathologic features. 2020 Vol. 50 (1): 41-44 [Abstract] ( 1846 ) HTML (1 KB)  PDF (1542 KB)  ( 818 ) 51 The effect of botulinum toxin A on the expression of inflammatory neurotransmitters in mice with postherpetic neuralgia LI Guosong, TANG Yanghua, XU Canda, SHEN Zhi’en, LIN Chengying DOI: 10.3969/j.issn.2095-9400.2020.01.010 Objective: To explore the mechanism of botulinum toxin A (BTX-A) on postherpetic neuralgia (PHN) by observing its effect on the expression of inflammatory neurotransmitter in mice with PNH. Methods: Thirty-six male C57 mice with PHN were randomly divided as group A, B and C, with 12 mice in each group. Herpes site in group A was injected with 0.9% saline, with BTX-A (2 U/kg) in group B, while BTX-A (2 U/kg) was injected into dorsal root ganglion (DRG) of the fifth lumber spine in group C. All mice were sacrificed after 7 d of continuous intervention, and DRG and corresponding segments of spinal cord were isolated from mice. The mRNA expression levels of TNF-α, IL-1β and neurokinin A in DRG and spinal cord was detected by RT-qPCR and ELISA. The expression of Na1.3 and Na1.8 channels in DRG and spinal cord was detected by Western blot. Results: RT-qPCR results showed that the mRNA expression levels of inflammatory neurotransmitters on TNF-α, IL-1β and neurokinin A in group B and group C were significantly lower than those in group A (P<0.05), but there was no significant difference between group B and group C. The results of ELISA showed that the protein contents of TNF-α, IL-1β and neurokinin A in group B and group C were significantly lower than those in group A (P<0.05), but there was no significant difference between group B and group C. Western blot results showed that the expression of Na1.3 and Na1.8 channel proteins in group B and group C decreased significantly compared with group A (P<0.05), but there was no statistical difference between the two groups. Conclusion: BTX-A can effectively inhibit PHN, and its mechanism may be by reducing the expression of Na1.3 and Na1.8 channel proteins and thus inhibiting the expression of inflammatory neurotransmitters TNF-α, IL-1β and neurokinin A. 2020 Vol. 50 (1): 51-55 [Abstract] ( 691 ) HTML (1 KB)  PDF (1615 KB)  ( 684 ) 56 The effect of ADMIRE-based low-tube voltage CT scanning on radiation dose and image quality of the upper abdomen CHEN Chunmiao, LIN Guihan, WU Xulu, LU Chenying, YANG Hongyuan, CHEN Xiao, JI Jiansong, HU Xianghua DOI: 10.3969/j.issn.2095-9400.2020.01.011 Objective: To explore the effect of low-tube voltage CT scanning on radiation dose and image quality of the upper abdomen based on advanced model iterative reconstruction algorithm (ADMIRE). Methods: Totally 200 patients who had undergone upper abdomen scanning in Lishui Central Hospital from November 2016 to September 2017 were enrolled and randomly assigned to the control group (n=50) and the experimental group (n=150), who were all treated with ADMIRE with the tube voltage of 120 kV in the control group. Meanwhile, the experimental group was divided as low-tube voltage A, B, and C according to the body mass index (BMI) with the tube voltage of 80 kV, 90 kV and 100 kV respectively. Subjective scores of images, background noise standard deviation (SD), liver signal-to-noise ratio (SNR), liver contrast-to-noise ratio (CNR) and effective radiation dose (ED) were compared between each group. Results: There were no significant differences between the control group and all the low-tube voltage groups (P>0.05) in age, gender or CT values of erector spinal and the image quality scores, with all images meeting the clinical diagnostic requirements, but there were significant differences (P<0.05) in BMI and liver CT. The BMI of the low-tube voltage groups A and B was significantly lower than that of the control group, while that of the low-tube voltage group C was significantly higher than the control group. Liver CT value of the low-tube voltage group A was significantly higher than the control group. The background noise SD of the images of the low-tube voltage groups A, B, and C were significantly lower than those of the control group, while the liver SNR and liver CNR were significantly higher than those of the control group. Meanwhile, the ED of the low tube voltages A, B and C was significantly lower than that of the control group (P<0.05). Conclusion: When applying the third-generation dual-source CT to the upper abdominal scanning, ADMIRE-based low-tube voltage scanning mode can significantly reduce the radiation dose and ensure the image quality. 2020 Vol. 50 (1): 56-59,64 [Abstract] ( 668 ) HTML (1 KB)  PDF (1614 KB)  ( 853 ) 60 Clinical effect of fire acupuncture combined with balanced acupuncture in the treatment of knee osteoarthritis LIAO Huiyu, QU Chongzheng DOI: 10.3969/j.issn.2095-9400.2020.01.012 Objective: To compare the clinical efficacy of fire needle combined with balance needle versus the conventional needle in the treatment of knee osteoarthritis. Methods: A retrospective study was made of totally 67 patients with knee osteoarthritis received treated from June 2016 to August 2017 in the Orthopedics and Traumatology Department of the Third Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine. They were randomly classified into single acupuncture group (34 patients) and compound acupuncture group (33 patients). The single acupuncture group received the conventional filiform needle therapy, while the compound acupuncture group received fire needle combined with balanced needle therapy. VAS, WOMAC, and the comprehensive clinical efficacy evaluation were used as indicators to test the effectiveness of two therapeutic methods. t-test and χ2-test were used to compare the differences in curative effect between pre-treatment and post-treatment, as well as those the inter-group treatment efficiency. Results: Similarities were observed in age, sex, disease duration, VAS and WOMAC score between the groups before treatment (P>0.05). Conventional filiform needle therapy and combined needle therapy effectively reduced VAS and WOMAC scores of patients with knee osteoarthritis, indicating that both methods effectively alleviated the condition of knee osteoarthritis patients (P<0.05). Compared with single filiform needle therapy, combined needle therapy was more effective in relieving pain, stiffness of bone and joint, and disturbance of movement (P<0.05). Besides, although the overall efficacy of the two methods were similar (P>0.05), the curative effect of combined-needle therapy on knee osteoarthritis was significantly higher than that of single needle method (P<0.05). Conclusion: The clinical effectiveness of fire needle combined with balanced needle was better than that of conventional needling method in the treatment of knee osteoarthritis. 2020 Vol. 50 (1): 60-64 [Abstract] ( 807 ) HTML (1 KB)  PDF (1526 KB)  ( 958 ) 65 A clinical analysis of patients with infective endocarditic in hemodialysis Zheng Yiyi, Liu Yi, Guo Jiaowei DOI: 10.3969/j.issn.2095-9400.2020.01.013 Objective: To analyze the clinical features and outcomes of hemodialysis (HD) patients complicated with infective endocarditis (IE). Methods: Clinical data of 460 ESRD patients with hemodialysis admitted to the First Affiliated Hospital of Wenzhou Medical University from January 2013 to December 2017 were collected. Results: There were 9 patients with IE, with an average age of 58.2±13.8 years old and five males. The vascular access was long-term central venous catheter with an average dialysis time of 20 months. Fever was the common symptom (100%). Complications occurred in 6 cases: mainly cerebral infarction, heart failure and cerebral hemorrhage. All patients underwent blood culture examination, 7 cases were positive and the main bacteria ware staphylococcus (71.4%). Thoracic echocardiography (TTE) was used in 7 patients, Tran Esophageal Echocardiography (TEE) in 1 patient, and both TTE and TEE examined in 1 patient. Vegetations were found in nine patients. The most common involvement was mitral valve (5/9), followed by tricuspid valve (2/9). Eight patients were treated conservatively. One underwent surgical treatment. Four patients were discharged after improvement and five patients were discharge automatically. Conclusion: Hemodialysis with CVC is a risk factor of IE. Hemodialysis patients, especially dialysating with CVC should be considered infectious endocarditis if they have fever. Echocardiography and blood culture are two main methods for clarifying the diagnosis. 2020 Vol. 50 (1): 65-67 [Abstract] ( 817 ) HTML (1 KB)  PDF (1509 KB)  ( 653 ) 68 Clinical diagnosis in the early stage of infection with paragonimiasis of chest and lung type XIE Yibing, HONG Jialin, HUANG Youquan, XU Jingxuan, CHEN Qi, CHEN Xiaofang, XU Chongyong DOI: 10.3969/j.issn.2095-9400.2020.01.014 Objective: To investigate the clinical diagnosis in the early stage of infection with paragonimiasis of chest and lung type. Methods: Totally 62 cases of paragonimiasis in the early infection stage in Yongjia County People’s Hospital were included in the study. The clinical, laboratory and imaging findings of paragonimiasis were analyzed retrospectively. Results: Out of 62 cases, 50 complained of cold-like symptoms in their first vist, and all 62 had positive paragonimus antibody, with the eosinophil count in peripheral blood (5.51±3.06)×109/L, the proportion of eosinophil 35.6%±19.0%, an increase to a certain extentin the percentage of eosinophilic. 42 cases complicated with pleural effusion, 4 cases of simple pleural effusion, and 16 cases of intra-pulmonary lesions only. Conclusion: The possibility of paragonimiasis infection should be taken into consideration in the first diagnosis of patients with cold-like symptoms, elevated eosinophils and pulmonary lesions. 2020 Vol. 50 (1): 68-70 [Abstract] ( 834 ) HTML (1 KB)  PDF (1687 KB)  ( 692 ) 73 The effect of temperature control on rapid recovery of patients with gastric cancer after laparoscopic surgery WANG Yanmin, YE Lingxiao, ZHANG Liping DOI: 10.3969/j.issn.2095-9400.2020.01.016 Objective: To investigate the effect of heat preservation on rapid recovery of patients with gastric cancer after laparoscopic surgery. Methods: A total of 66 patients, who undertook laparoscopic subtotal gastrectomy in Ningbo Medical Treatment Centre Lihuili Hospital from January 2015 to October 2018, were randomly divided into the control group and the experimental group. The patients in the control group adopted routine perioperative nursing and the experimental group patients were kept warm during postoperative period. The two groups were statistically analyzed in terms of blood pressure, heart rate, ear temperature during the study, and postoperative extubation time, eating and anus exhaust time and postoperative hospitalization time. Results: Compared with the control group, the experimental group patients had higher ear temperature, slower heart rate, earlier eating time, shorter postoperative hospitalization and lower postoperative gastroplegia rate, with statistical difference between the two groups (P<0.05). Conclusion: The postoperative temperature control for laparoscopic subtotal gastrectomy patients with gastric carcinoma could effectively shorten the time of hospitalization with sooner postoperative recovery. 2020 Vol. 50 (1): 73-75 [Abstract] ( 775 ) HTML (1 KB)  PDF (1512 KB)  ( 706 )

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