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2019 Vol. 49, No. 12 Published: 2019-12-25   867 The protective effect of salvianolic acid B on oxidative damage of osteoblasts RONG Caili, SANG Chunhui, CHEN Dong, TANG Qi DOI: 10.3969/j.issn.2095-9400.2019.12.003 Objective: To explore the protective effect of salvianolic acid B (Sal B) on oxidative damage of osteoblasts. Methods: The oxidative damage model of osteoblasts induced by tert-butyl hydroperoxide (TBHP) was constructed. Cell viability was detected by MTT assay. The level of reactive oxygen species (ROS)was detected by DCFH-DA fluorescence staining. Real-time PCR was used to assess the levels of osteogenic differentiation genes. Alizarin red staining was used to detect the extracellular matrix mineralization of osteoblasts. Results: The results of MTT showed that the inhibition of osteoblast viability by TBHP was in a time- and concentration-dependent manner. Treatment with 250 μmol/L TBHP for 6 h was the half inhibitory condition for osteoblasts. Sal B significantly restored cell viability up to 84.6%, the expression of differentiation genes and extracellular matrix mineralization level of osteoblasts (P<0.001) and meanwhile decreased the level of ROS (P<0.001). Conclusion: Sal B effectively inhibited the oxidative damage and restored osteoblasts’ viability and function. 2019 Vol. 49 (12): 867-871,877 [Abstract] ( 652 ) HTML (1 KB)  PDF (1671 KB)  ( 795 ) 872 The role of inflammatory pathways in the pathogenesis of ankylosing spondylitis based on transcriptomics studies LI Chenlu, FANG Jinxia, LUO Jing, ZHU Xiaochun, WANG Xiaobing DOI: 10.3969/j.issn.2095-9400.2019.12.004 Objective: To study the transcriptomics of ankylosing spondylitis (AS) to elucidate the key genes and pathways involved in the pathogenesis of the disease. Methods: Peripheral blood mononuclear cells from AS patients and healthy controls were collected. RNA was extracted and gene expression profiles were obtained by high-throughput RNA sequencing. Bioinformatics method was used to compare the gene expressions of AS patients and healthy controls to find differentially expressed genes (DEGs). GO function annotation was performed, and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment as well as protein interaction network (PPI) were analyzed. The levels of important cytokines involved in the pathway were further verified in the peripheral blood of AS patients and healthy controls by Enzyme-linked immunosorbent assay (ELISA). Results: We found 153 differentially expressed genes (DEGs) in AS patients and healthy controls, of which 149 genes were up-regulated and 4 genes down-regulated, and all of them were closely related to each other. The biological function of the up-regulated gene was found to be mainly related to inflammatory pathways such as TNF signaling pathway and chemokine signaling. In addition, it was confirmed in peripheral plasma that the expression levels of inflammatory cytokines IL-2, IL-6, IL-10, TNF-α and IFN-γ were significantly higher in AS patients than in healthy controls (P<0.05). Conclusion: This study reveals important inflammatory genes and signaling pathways that play a role in the pathogenesis of ankylosing spondylitis, which provides a reference for targeted anti-inflammatory therapy in AS patients. 2019 Vol. 49 (12): 872-877 [Abstract] ( 797 ) HTML (1 KB)  PDF (1849 KB)  ( 865 ) 878 The mechanism of curcumin in regulating glycolysis of endometrial carcinoma cells JIN Weiwei, LIN Yihe, ZHAO Xiaoying, ZHUANG Xiaoping, CAI Pingsheng DOI: 10.3969/j.issn.2095-9400.2019.12.005 Objective: To detect the energy metabolic pathway of curcumin combined with 5-Fluorouridine and its killing effect on endometrial cancer cells. Methods: The levels of glycolysis and oxidative phosphorylation in endometrial cancer cells were measured by Seahores and Lactate/Pyruvate assay kit to confirm the main metabolic pathway in the endometrial cancer cells. Metabolic genes in Ishikawa and curcumin treated Ishikawa cells were screened by real-time PCR to explore the candidate target gene of curcumin; the level of glycolysis and oxidative phosphorylation were detected by Seahorse and the lactate/pyruvate assay in PDK2-knockout cells to reveal how curcumin regulated the energy metabolic pathway in endometrial carcinoma cells via PDK2. We establish human endometrial cancer model in nude mice to detect the treatment effect of 5-Fluoracil combined with curcumin in vivo. Results: It was revealed that curcumin could inhibit the glycolysis in the endometrial cancer cells, but the OXPHOS level couldn’t be changed. It was also found that curcumin could down-regulate the expression of pyruvate dehydrogenase kinase-2 (PDK2), which regulated the process of glycolysis. The knock-downed expression of PDK2 by specific shRNA decreased the level of glycolysis and proliferation of endometrial cancer cells. Curcumin could inhibit the level of glycolysis in the endometrial cancer cells by down-regulating the expression of PDK2. Combined with 5-FU, curcumin could obviously increase the sensitivity of endometrial cancer cells to chemotherapeutics, and up-regulate the ability to inhibit the proliferation of cancer cells. Conclusion: Curcumin could inhibit the expression of PDK2 and the level of glycolysis in the endometrial cancer cells. As a result, curcumin could obviously increase the sensitivity to 5-FU, and inhibit the proliferation of endometrial cancer cells effectively. 2019 Vol. 49 (12): 878-884 [Abstract] ( 966 ) HTML (1 KB)  PDF (1684 KB)  ( 842 ) 885 The effect of RNA interference gene SALL4 on proliferation and apoptosis of testicular yolk sac tumor cells in children LYU Min, CAI Zuren, CHEN Xiaoming DOI: 10.3969/j.issn.2095-9400.2019.12.006 Objective: To study the effect of RNA interference gene SALL4 on the proliferation and apaptosis of testicular yolk sac tumor cells in children. Methods: The pGPU6/GFP/neo-sall4 shRNA was constructed as a vector to be transfected into pediatric testicular yolk sac tumor cells and interfere in SALL4. The interfering effect was verified by detecting mRNA and protein levels. CCK-8 and flow cytometry were employed to study the inhibition effect of interfering SALL4 in pediatric testicular yolk sac tumor cells. Results: After successfully transfecting the vector into pediatric testicular yolk sac tumor cells, the mRNA expression magnitudes of SALL4 interfered group was 24.0%±4.0%, as compared with Negative group (93.0%±43.0%), with lower levels of mRNA expression (P<0.05). And the protein expressions of SALL4 interfered group (0.39±0.10) had a lower protein expression than Negative group (1.02±0.09). Besides, the proliferation was significantly reduced, while the apoptosis rate was significantly increased in vitro (P<0.05). Conclusion: Targeting SALL4 RNA could inhibit the proliferation and induce apoptosis of pediatric testicular yolk sac tumor cells, suggesting that SALL4 may be a potential therapeutic target for pediatric testicular yolk sac tumor. 2019 Vol. 49 (12): 885-888,893 [Abstract] ( 628 ) HTML (1 KB)  PDF (1704 KB)  ( 707 ) 889 Preparation technology of the trauma swelling elimination ointment and determination of its active components ZHANG Hong, WANG Xiaofeng, HUANG Shuxian DOI: 10.3969/j.issn.2095-9400.2019.12.007 Objective: To optimize the preparation technology of trauma swelling elimination and painkiller ointment and to determine the content of its active components. Methods: An orthogonal experiment was designed to optimize the ethanol extraction process. The single factor method was used to optimize the preparation process. The content of emodin and polydatin was determined by HPLC. Results: The best alcohol extraction process was to extract 7 times the amount of 70% ethanol three times, 2 hours each. The viscosity of the paste was moderate when the extraction was 3 times the amount and the ratio of gelatin to Arabic gum was 1:4. The content of emodin and polydatin was 4.14 μg·g-1 and 12.71 μg·g-1. Conclusion: The optimized process is stable and feasible, and thus suitable for the preparation of the trauma swelling elimination and painkiller ointment. The index components of emodin and polydatin were stable and feasible, which can be used for quality control. 2019 Vol. 49 (12): 889-893 [Abstract] ( 557 ) HTML (1 KB)  PDF (1519 KB)  ( 754 ) 894 An analysis of neonatal diseases and causes of death in a neonatal department from 2010 to 2017 CHEN Si, XIAO Xiuman, LIU Yanli, YOU Yaya, JIN Linghuan, CHEN Shangqin DOI: 10.3969/j.issn.2095-9400.2019.12.008 Objective: To investigate the neonatal diseases and main causes for death in our neonatal department from 2010 to 2017. Methods: The clinical data of neonates who were admitted from January 2010 to December 2017 were retrospectively collected. The data were divided into two groups according to the year of admission (2010-2013 and 2014-2017). Incidence and mortality of major diseases were compared between the two groups. Results: A total of 25 632 neonates were included in the study. The data of the two groups (2010-2013 vs. 2014-2017) were compared. The mortality rate significantly decreased from 2.19% to 1.66% (P=0.001) and the mortality rate in neonates with GA<28 weeks (25.32% vs. 15.77%, P=0.022) and GA≥37 weeks (1.64% vs. 1.02%, P<0.001) decreased significantly. The incidences of the following complications in premature infants increased: NRDS (9.43% vs. 10.76%, P<0.001), BPD (2.05% vs 3.40%, P<0.001), ROP (1.51% vs. 2.82%, P<0.001), NEC (1.25% vs. 1.90%, P<0.001), PVL (0.77‰ vs. 2.57‰, P=0.001), and the following incidence of birth defects was on the rise: congenital heart disease (16.97% vs. 19.72%, P<0.001), digestive/abdominal malformation (4.19% vs. 5.13%, P=0.001). The incidence decreased in neonatal asphyxia (5.12% vs 3.33%, P<0.001), and neonatal hypoxic-ischemic brain damage (2.74% vs. 1.46%, P<0.001). On the other hand, the mortality of infection (0.89% vs. 0.21%, P<0.001), preterm NEC (30.95% vs. 17.26%, P=0.008) and sepsis (3.07% vs. 1.15%, P=0.014) decreased significantly. In addition, the mean gestational age (GA) of the test tube baby was lower than that of spontaneous pregnant baby (32.67±0.12 weeks vs. 36.99±0.02 weeks, P<0.001), with higher mortality rate (4.26% vs. 1.79%, P<0.001). Conclusion: There was a decreasing trend in the overall mortality and mortality rate for infectious diseases from 2010 to 2017. As the survival rate of premature infants was increasing, it is of vital importance to take preventive and therapeutic measures to combat the complications of premature infants. The mean GA of test tube baby was significantly lower than that of natural conception yet the mortality was much higher. With the increased incidence of birth defects, which has become one of the major causes of death, further multidisciplinary collaboration is needed to reduce the birth defect potential. 2019 Vol. 49 (12): 894-899,904 [Abstract] ( 695 ) HTML (1 KB)  PDF (1592 KB)  ( 935 ) 900 Risk factors associated with anemia in patients who received linezolid therapy DAI Ying, JIANG Shuying, YU Xuben, ZHANG Chunhong, HU Lufeng, LIN Guanyang DOI: 10.3969/j.issn.2095-9400.2019.12.009 Objective: To investigate the factors affecting linezolid-induced anemia in hospitalized patients.Methods: A retrospective study was performed to collect data in 161 adult inpatients who received linezolid between May 2012 to May 2017. The risk factors of linezolid-related anemia were confirmed by univariate and multivariate logistic regression. Results: Of all the patients enrolled, linezolid-induced anemia developed in 31 (19.2%) patients during therapy. The incidence of anemia occurred more frequently in patients who received linezolid at concentration≥7 mg·L-1 (OR=2.87, 95%CI: 1.11-6.93, P=0.024) and those with moderate-severe renal impairment [GFR<60 mL·(min·1.73 m2)-1] (OR=3.47, 95%CI: 1.49-8.13, P=0.004). Conclusion: The results of our study indicated that high plasma linezolid concentration and renal impairment significantly affected the development of linezolid-induced anemia. 2019 Vol. 49 (12): 900-904 [Abstract] ( 831 ) HTML (1 KB)  PDF (1534 KB)  ( 687 ) 905 The role of Calpain 1 in remifentanil-induced pain allergy in rats HUANG Yan, ZHOU Nan DOI: 10.3969/j.issn.2095-9400.2019.12.010 Objective: To investigate the role of calpain 1 (CANP1) and its related signaling pathway in hyperalgesia induced by remifentanil in rats with incisional pain. Methods: Incisional pain model was established in SD rats qualified for tail vein and sheath tube. The control group, remifentanil group, remifentanil+U0126 group and remifentanil+Calpeptin group were established. Remifentanil induced model incision pain. Pretreatment with MEK kinase inhibitor U0126 inhibited MEK activity in the spinal cord. Calmodulin (CANP)-specific inhibitor Calpeptin pretreatment intervened with CANP activity in the spinal cord. Mechanical contraction threshold (MWT) and heat-shrinking latency (TWL) were measured 24 h before intravenous infusion of saline or remifentanil, 2, 6, 24, and 48 h after intravenous administration (T0-T4). The mechanical contraction threshold (MWT) and heat-shrinking latency (TWL) were measured at 24 h before intravenous administration and 2, 6, 24, 48 h after intravenous administration (T0-T4). The protein expression levels of ERK, Calpain1 and GSK-3β were detected by Western blot. Results: There was no significant change in MWT and TWL in the control group at T0-T4. The MWT of the rats in the remifentanil group gradually decreased, and the TWL gradually shortened. The difference in the time points between the two groups was statistically significant (P<0.01). Compared with the remifentanil group, both the remifentanil+U0126 group and the remifentanil+calpeptin group had higher MWT and TWL at each time point (P<0.05 or P<0.01). Compared with the control group, the expression of p-ERK protein and CANP1 large subunit hydrolyzed protein (Cleaved-CANP1) was significantly up-regulated in the remifentanil group (P<0.01). Compared with the remifentanil group, the expression of p-ERK protein was significantly down-regulated in the remifentanil+U0126 group (P<0.01), and there was no significant change in the remifentanil+Calpeptin group. Compared with the control group, the relative protein expressions of GSK-3β and pGSK-3β/GSK-3β in the remifentanil group were significantly increased (P<0.01). Compared with the remifentanil group, the relative protein expression levels of GSK-3β and pGSK-3β/GSK-3β were significantly decreased in the remifentanil+U0126 group and remifentanil+Calpeptin group (P<0.01). Conclusion: The pain allergic effect induced by remifentanil may be related to the activation of ERK/CANP1/GSK-3β signaling pathway. 2019 Vol. 49 (12): 905-909 [Abstract] ( 699 ) HTML (1 KB)  PDF (1766 KB)  ( 624 ) 910 The significance of CA-125 level in atrial fibrillation CHEN Chen, HU Xiaokang, WANG Kaijing, HUANG Zhouqing DOI: 10.3969/j.issn.2095-9400.2019.12.011 Objective: To investigate the significance of CA-125 in patients with paroxysmal and persistent atrial fibrillation (AF). Methods: A retrospective study was made of 642 patients admitted to the First Affiliated Hospital of Wenzhou Medical University (China) from June 2017 to June 2018. All subjects enrolled were divided into two groups according to episode/persistent AF and they were compared in paroxysmal AF and persistent AF. Their clinical data (age, gender, BMI, duration of AF, left atrial diameter, ejection fraction, BNP, liver and kidney function, CHA2DS2-VASc score, CA-125, etc.) were analyzed to identify the risk factors of persistent AF by multivariate regression analysis. Results: Statistical regression results showed that patients with persistent AF had higher levels of CA-125 (P<0.001) compared with patients with paroxysmal AF. Logistic regression analyses showed that CA-125 levels appeared to be unaffected by age, LAD, ejection fraction, sex and so on. Conclusion: Patients with persistent AF have higher CA-125 level. 2019 Vol. 49 (12): 910-913 [Abstract] ( 631 ) HTML (1 KB)  PDF (1520 KB)  ( 932 ) 914 Clinical characteristics of group B streptococci infection in genital tract of puerperae YANG Xunjun, DING Hongxiang, NI Li DOI: 10.3969/j.issn.2095-9400.2019.12.012 Objective: To investigate the colonization of obstetric group B streptococcus (GBS) in pregnant women in the late pregnancy and its effect on the pregnancy outcome of perinatal pregnant women. Methods: A total of 3 619 pregnant women who underwent regular check-up in the Second Affiliated Hospital of Wenzhou Medical University from May 2018 to March 2019 were enrolled to be indentified for GBS infection. Query electronic medical records were set up to understand the high maternal risk factors to judge the pregnancy outcome according to clinical diagnosis and routine examination of leucorrhea. Data were analyzed by statistical methods. Results: A comparison between the two groups showed that the history of fungal infection or bacterial vaginitis in GBS-positive group was significantly higher than that in negative group. The results of vaginal secretion test showed that GBS positive maternal vaginal pH increased, cleanliness was worse, and the detection rate of fungi and clue cells was higher (P<0.05, respectively). History of fungal infection and history of bacterial vaginitis were risk factors for GBS infection (P<0.05). According to pregnancy outcome, the incidence of premature rupture of membranes, intrauterine infection and neonatal infection was significantly higher in GBS-positive group (P<0.05). Conclusion: The history of fungal infection or bacterial vaginitis is a high risk factor for GBS infection, which can have adverse effects on maternal and neonatal outcomes and can also have a negative impact on maternal vaginal microenvironment. 2019 Vol. 49 (12): 914-917 [Abstract] ( 628 ) HTML (1 KB)  PDF (1501 KB)  ( 665 ) 918 The application of digital orthopedics technology in the individualized treatment of thoracolumbar fractures YANG Yu, XU Wei, JIN Gang, LIU Dapeng, KAISAIERJIANG·Aihamati DOI: 10.3969/j.issn.2095-9400.2019.12.013 Objective: To explore the application value of digital orthopedics technology in the individualized treatment of thoracolumbar fractures. Methods: A total of 10 patients with thoracolumbar vertebral fracture were enrolled from December 2015 to June 2017, who had to be operated on internal fixation of thoracolumbar fractures. The DICOM data were obtained preoperatively by means of CT scan. Injured and surrounding vertebra were reconstructed in patients with the aid of digital orthopedics technology and related software (Mimics 17.0, Geomagic studio12.0, Unigraphics NX10.0, Rapid prototyping technology). According to the related vertebral sclerotin condition in three-dimensional space, the operative plan was made, the nail canal designed, the navigation template done and the operation procedure simulated. Intra-operative thoracolumbar vertebral fracture operations were implemented with pedicle navigation template. The postoperative effect of screw placement and its complications were recorded. Results: The thoracolumbar vertebral fracture operations in 10 cases were successfully completed. Totally 64 pedicle screws were placed. In accordance with the Gertzbein and Robbins rating standard: 53 cases of pedicle screws were Grade I, 9 were Grade II and 2 were Grade III, with an accuracy of screw placement being 96.9% (62/64). All patients received 12-24 months’ follow-up, average time 13.0±6.3 months, and no complications related to screw placement such as screw false placement, spinal cord injury, internal fixation failure and so on were found. Conclusion: Digital orthopedics technology provides a kind of new change and method for the individualized and lean treatment of thoracolumbar fractures. 2019 Vol. 49 (12): 918-921 [Abstract] ( 600 ) HTML (1 KB)  PDF (1646 KB)  ( 888 ) 922 Internal fixator with iliolumbar fixation in treating vertically unstable pelvic fracture LI Xiang, HE Long, LIANG Yimin, LI Yonghua, PAN Hansong, ZENG Li DOI: 10.3969/j.issn.2095-9400.2019.12.014 Objective: To evaluate the clinical outcomes of Internal fixator (INFIX) with iliolumbar fixation in treating vertically unstable pelvic fracture (Tile C). Methods: The clinical data of 5 patients with a vertically unstable pelvic fracture were treated with INFIX with iliolumbar fixation from May 2016 to April 2017 were retrospectively analyzed. There were 2 males and 3 females, aged from 29 to 65 years old with an average of (42.3±14.8) years. Among them, five fractures were classified as type C according to the classification of Tile. This fixation construct comprised a vertical lumbopelvic distraction component which fixed L4, 5 and the posterior superior iliac spine and percutaneous internal fixator using the vertebral pedical screwrod system which fixed anterior inferior iliac spine. Vertical displacement and functional outcome were evaluated by Matta method and Majeed score criteria respectively, and fracture healing time, nerve function, clinical function and complications were documented. Results: All the patients were followed up for 10 to 16 months. In the present clinical series, all fractures healed without significant loss of reduction. There were 3 excellent, 2 good according to the Matta criteria. There were 1 excellent, 3 good and 1 fair according to the Majeed score evaluation by the end of last follow-up visit. No iatrogenic complications of neurovascular injury occurred. Patients now walk unassisted without pain in the waist or legs, and with no shortening of lower limbs or claudication. Conclusion: INFIX with iliolumbar fixation that allows early mobilization and ambulation, with general applicability and definite safety, is an effective surgical technique for the treatment of vertically unstable pelvic fracture. 2019 Vol. 49 (12): 922-925,929 [Abstract] ( 560 ) HTML (1 KB)  PDF (1623 KB)  ( 777 ) 926 An evaluation of the quality of c-shaped root canal obturation using cone-beam CT and digital periapical radiography LYU Hanxiao, WANG Min, SUN Yu, DU Yu, PAN Yihuai DOI: 10.3969/j.issn.2095-9400.2019.12.015 Objective: To compare the quality of root canal obturation of C-shaped canal in mandibular second molar by using cone beam computerized tomography (CBCT) and digital periapical radiography (DPR) to assess the advantages and application value of CBCT in C-shaped root canal obturation. Methods: A total of 82 patients (86 mandibular second molars) received root canal therapy in endodontics department. After root canal obturation, the length and density of root filling were assessed with CBCT and DPR simultaneously by two experienced endodontists. Chi-square test was performed to compare the difference between CBCT and DPR in evaluating the quality of the root obturation. Results: With the same root canal preparation and obturation methods, the results varied significantly due to different postoperative evaluation methods. In terms of length and density of root obturation, CBCT was significantly better than of DPR (P<0.05). Conclusion: CBCT is more reliable than DPR in evaluating the obturation quality of C-shaped root canal in mandibular second molar, and has extensive application value in the evaluation of anatomic complex root canal system. 2019 Vol. 49 (12): 926-929 [Abstract] ( 648 ) HTML (1 KB)  PDF (1558 KB)  ( 906 ) 930 Correlation between cd42b, cd62p, Hcy, hs-CRP and the severity of coronary artery disease in patients with acute myocardial infarction FENG Yan, LI Yanmin, CHAI Liangting, LI Jing, ZHANG Yan DOI: 10.3969/j.issn.2095-9400.2019.12.016 Objective: To investigate the correlation between cd42b, cd62p, homocysteine (Hcy) and high-sensitivity C-reactive protein (hs-CRP) and severity of coronary artery disease in patients with acute myocardial infarction (AMI). Methods: Totally 154 patients with AMI were enrolled. According to the Gensini score, they were divided as L group (≤55 points), M group (56-84 points) and H group (85-172 points). The differences in levels of cd42b, cd62p, Hcy and hs-CRP were compared between the three groups. The differences in cd42b, cd62p, Hcy and hs-CRP levels were compared between patients with different coronary lesions. The correlation between cd42b, cd62p, Hcy and hs-CRP and the extent of coronary lesions was analyzed. Results: The levels of cd62p, Hcy and hs-CRP were significantly different between groups (P<0.05). The cd62p, Hcy and hs-CRP levels in group M were significantly higher than those in group L (P<0.05). The levels of cd62p, Hcy, hs-CRP and hs-CRP were significantly higher in group H than those of L and M groups (P<0.05). There was no significant difference in cd42b levels between groups. The levels of cd62p, Hcy and hs-CRP in different lesions were significantly different (P<0.05). The levels of cd62p, Hcy and hs-CRP in patients with 2 and 3 lesions were significantly higher than those with 1 lesion (P<0.05). The levels of cd62p, Hcy and hs-CRP in patients with 3 lesions were significantly higher than those with 2 lesions (P<0.05). With the increase of Gensini score, cd42b showed a downward trend, with group L the highest level of cd42b, and group H the lowest level of cd42b. There was a significant positive correlation between cd62p, Hcy and hs-CRP and Gensini score (P<0.05). Conclusion: cd62p, Hcy and hs-CRP are significantly positively correlated with the degree of coronary artery lesions in patients with AMI, suggesting the detection of cd62p, Hcy and hs-CRP levels could be used to understand the degree of coronary artery lesions in AMI patients. 2019 Vol. 49 (12): 930-933 [Abstract] ( 612 ) HTML (1 KB)  PDF (1505 KB)  ( 839 )

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