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2019 Vol. 49, No. 8 Published: 2019-08-25   547 Preparation and identification of human cytomegalovirus US31 protein specific antibody CHEN Bi, XIE Shangdan, XIANG Chaoli, SHI Xinyu, GUO Gangqiang, OUYANG Jingzhong, SHEN Xian DOI: 10.3969/j.issn.2095-9400.2019.08.001 Objective: To explore the preparation and identification of human cytomegalovirus (HCMV) US31 protein and its polyclonal antibody. Methods: After HCMV US31 gene was optimized by prokaryotic codon for whole gene synthesis and cloned to pET21a (+) prokaryotic expression vector, pET21a (+)/HCMV US31 recombinant plasmid was constructed, with identified sequence. Recombinant plasmid was transformed into Escherichia coli BL21 (DE3), target protein expression induced by IPTG, and US31 protein purified by Ni-NTA affinity chromatography. Immunized New Zealand rabbits were used to prepare polyclonal antibodies, and antibody titer and specificity was detected by ELISA, Western blot and immunofluorescence method. Results: High expression of HCMV US31 fusion protein was induced in Escherichia coli, and high purity target protein was obtained by nickel column affinity purification, and the specific IgG antibody was induced by immune New Zealand rabbits, with the valence being 1:49 600. ELISA, Western blot and immunofluorescence have confirmed that the prepared specific antibodies were able to identify US31 proteins expressed in cells. Conclusion: HCMV US31 protein and specific antibodies were successfully prepared, which laid foundations for further study of the biological function of US31 protein. 2019 Vol. 49 (8): 547-551 [Abstract] ( 834 ) HTML (1 KB)  PDF (1330 KB)  ( 768 ) 552 The optimization of coronary CT images by using the filter function 56 sharpness algorithm MAN Yi, ZHENG Kuikui, WANG Zhenzhang, LI Na,CHEN Weijian, CAO Guoquan DOI: 10.3969/j.issn.2095-9400.2019.08.002 Objective: To investigate the improvement of coronary artery CT angiography (CCTA) images by using the filter function 56 reconstruction technique under 320-row CT. Methods: We compared the spatial resolution between the filter function 56 sharpness algorithm reconstruction (FR) images and the routine reconstruction (RR) images by scanning the Catphan 500 phantom. 55 patients with coronary heart disease who underwent invasive coronary angiography (ICA) and CCTA in the First Affiliated Hospital of Wenzhou Medical University were included in the study. The differences of the evaluation for the degree of coronary artery stenosis between FR and RR images were retrospectively analyzed. Results: The result of the phantom CT scan suggested that the spatial resolution of FR could reach 9l p/cm, and that of RR was just 7l p/cm. In 55 patients, 709 segments of the coronary segments were included in our study. With ICA as the gold standard, 159 stenosis segments were found in our study. The coronary stenosis score on the FR image was more accurate than the RR image. There was no significant difference between the results of FR and ICA (P>0.05), and the results of RR were statistically different from ICA (P<0.05). The coronary stenosis score of the FR image was highly consistent with the ICA results (Kappa≥0.8). For the stenotic coronary segments with the diameter less than 1.5 mm, although there was no statistically significant difference between the FR images and the RR images, the FR image was still highly consistent with ICA (0.6≤Kappa<0.8). Conclusion: The FR technique under 320-row CT can effectively improve the spatial resolution of the images and improve the diagnostic accuracy of the CCTA. 2019 Vol. 49 (8): 552-556 [Abstract] ( 845 ) HTML (1 KB)  PDF (1933 KB)  ( 810 ) 557 The effect of Fasudil on degradation of chondrocyte matrix and its mechanism JI Encheng, Zhou Yeli, Chen Chengwei, Chen Chun, Li Jing, Gao Weiyang, Pan Zhe’er DOI: 10.3969/j.issn.2095-9400.2019.08.003 Objective: To study the effect of Fasudil on matrix degradation of humolan chondrocytes and to explore its possible mechanism. Methods: CCK-8 was used to determine whether Fasudil was toxic and to determine the appropriate drug concentration. The cells were grouped as control group, Fasudil group, IL-1β group and Fasudil+IL-1β group. NO levels were determined by nitrate reductase assay; Western blot was used to detect COX-2, iNOS, MMP-13, p-MYPT, ROCK1, ROCK2, p-Erk/Erk, p-JNK/JNK and p-p38/p38 levels; qRT-MMP-13 and collagen-II expressions were determined by PCR; cell immunofluorescence was used to detect the expressions of MMP-13. Results: Compared with the IL-1β group, ROCK1, ROCK2 and p-MYPT were inhibited in the Fasudil+IL-1β group; the expression of COX-2, NO, iNOS and MMP-13 was inhibited, so did the expression of p-Erk, p-JNK and p-p38; the phosphorylation levels of Erk, JNK and p38 were decreased, and the expression of collagen-II was up-regulated. The differences were statistically significant (P<0.05). Conclusion: Fasudil inhibited IL-1β-induced degradation of chondrocyte matrix, which is probably achieved by inhibiting MAPK signaling pathway. 2019 Vol. 49 (8): 557-562 [Abstract] ( 758 ) HTML (1 KB)  PDF (1943 KB)  ( 814 ) 563 The role of cannabinoid type 2 receptor in mediating osteogenic differentiation induced by hypoxia in rat bone mesenchymal stem cells HU Xuzhi, SHI Xinlian, DENG Hui DOI: 10.3969/j.issn.2095-9400.2019.08.004 Objective: To explore whether cannabinoid type 2 receptor (CB2) participates in the regulation of BMSCs osteogenic differentiation and how it is regulated by hypoxic microenvironment. Methods: The whole bone marrow cell adherence method was used to isolate and culture rBMSCs. Low-oxygen model was established by using different concentrations of chemical oxygen peroxide cobalt chloride (CoCl2). Real-time PCR and Western blotting were used to detect the expression of osteogenesis key proteins RUNX2 and OCN at the gene and protein levels. Further, combined with the CB2 inhibitor AM630, the role of CB2 in the osteogenic differentiation of rBMSCs in a hypoxic microenvironment was investigated. Results: Compared with the control group, the expression of RUNX2 and OCN mRNA and protein in rBMSCs increased significantly at 24, 48, 72, 96 h after hypoxia treatment (P<0.05); Oxygen treatment up-regulated mRNA and protein expression of CB2 (P<0.05). CB2 inhibitor AM630 inhibited the expression of RUNX2 and OCN induced by hypoxia (P<0.05). Conclusion: CB2 is involved in the regulation of osteogenic differentiation of rBMSCs promoted by hypoxia. 2019 Vol. 49 (8): 563-567 [Abstract] ( 732 ) HTML (1 KB)  PDF (1554 KB)  ( 742 ) 568 The way how miR-132 inhibits the migration, invasion and epithelial-mesenchymal transition of ostepsarcoma cells by targeting HMGA2 JIANG Zhen, ZHANG Shuijun, XU Jifeng, ZHAO Xiaobo, BAO Tao DOI: 10.3969/j.issn.2095-9400.2019.08.005 Objective: To investigate the effect of miR-132 on the migration, invasion and epithelial-mesenchymal transition (EMT) of osteosarcoma (OS) cells and explore the potential molecular mechanisms. Methods: The expression levels of miR-132 in human OS cells (U2OS and HOS) and human osteoblast hFOB1.19 were evaluated by quantitative real-time quantitative PCR (qRT-PCR). miR-132 mimic and miR-132 inhibitor were transfected into U2OS and HOS for the overexpression and knockdown of miR-132, respectively. qRT-PCR was employed to examine the efficacy of transfection. The effect of miR-132 on the migration, invasion and EMT of OS cells was detected by Transwell assay, qRT-PCR and Western blot. Bioinformatics website TargetScan was searched to identify the potential targets of miR-132 and dual luciferase reporter assay was performed to validate the interaction between miR-132 and HMGA2.Real-time quantitative PCR and Western blot were used to confirm the regulatory effect of miR-132 on HMGA2 expression. Results: The expression of miR-132 in OS cells was significantly decreased compared with that in hFOB1.19 cells (P<0.05). Transfection of miR-132 mimics significantly increased the expression level of miR-132 in U2OS cells (P<0.01). miR-132 overexpression led to decreased ability of cell migration and invasion, and increased E-cadherin level and decreased Vimentin level (P<0.05). Knockdown of miR-132 by transfection of miR-132 inhibitor resulted in enhanced metastatic ability, decreased E-cadherin expression and increased Vimentin expression (P<0.05). Data mining of bioinformatics website Targetscan suggested HMGA2 was a potential downstream target of miR-132. Dual luciferase reporter gene assay confirmed miR-132 could interact with HMGA2 3’-UTR. Overexpression of miR-132 significantly decreased HMGA2 expression while miR-132 knockdown led to increased expression of HMGA2 (P<0.05). Conclusion: The expression of miR-132 is decreased in OS cells, and miR-132 inhibits the migration, invasion and EMT of HCC cells by down-regulating the expression of HMGA2. 2019 Vol. 49 (8): 568-574 [Abstract] ( 687 ) HTML (1 KB)  PDF (1473 KB)  ( 951 ) 575 Preparation, characterization and in vitro observation of HER2 targeted polymer microbubbles as a contrast agent for molecular imaging CHENG Yueyue, YU Fangfang, YANG Yan, WU Senmin, LI Jiaping DOI: 10.3969/j.issn.2095-9400.2019.08.006 Objective: To prepare HER2 receptor targeted polymer microbubbles for contrast-ultrasound imaging and to evaluate their physicochemical properties, tumor targeting performance and ultrasonic imaging effect in vitro. Methods: HER2-targeted polymer microbubbles were synthesized by biotin-avidin method. The particle size, potential and stability were investigated. In vitro tumor targeting experiment, its targeting ability to HER2 (+) breast cancer cell BT-474 and HER2 (-) breast cancer cell MDA-MB-231 was investigated. The ultrasonic imaging ability was observed in vitro. Results: The average particle size of HER2-targeted polymer microbubbles was (1.78±0.37) μm, the average surface Zeta potential was (-37.40±5.74) mV. There was no statistical significant difference in the particle size of the targeted polymer microbubbles within 12 h (P>0.05). HER2-targeted polymer microbubbles exhibited the remarkable targeting ability to HER2 (+) tumor cells  in vitro experiment. The microbubbles presented fine and uniform point-like dense hyperecho in vitro ultrasound imaging. Conclusion: HER2-targeted polymer microbubbles have good stability, strong targeting ability and satisfactory effect in vitro imaging, which lays a good foundation for further study of tumor targeted ultrasound imagine in vivo. 2019 Vol. 49 (8): 575-580 [Abstract] ( 796 ) HTML (1 KB)  PDF (1552 KB)  ( 926 ) 581 Construction of composite scaffolds for cartilage repair WANG Yingying, LI Huaqiong, ZHAO Allan Zijian DOI: 10.3969/j.issn.2095-9400.2019.08.007 Objective: To construct decellularized cartilage/GelMA composite scaffolds for repairing the cartilage defects. Methods: The α-1, 3-galactosidase gene knockout (GTKO) porcine costal cartilage was decellularized to obtain low immunogenic decellularized cartilage matrix. DAPI staining and DNA content detection were used to evaluate the effect of decellularization. HE staining and Masson trichrome staining were used to evaluate the extracellular matrix structure and protein retention. The decellularized cartilage was frozen and ground into powder, the polymer gelatin methacryloyl (GelMA) solution and decellularized cartilage powder were uniformly mixed in different proportion to prepare an injectable biomaterial, then obtained biodegradable composite scaffolds by ultraviolet crosslinking. Finally, the scaffolds were characterized by swelling performance, storage modulus and cell proliferation. Results: The DNA content of natural cartilage was 161.2 ng/mg, and the decellularized cartilage was only 15.27 ng/mg, so this method had a good effect on decellularization. HE staining showed that the extracellular matrix structure was intact, and Masson trichromatic staining showed that most of the extracellular matrix components were retained in decellularized cartilage. Compared with pure GelMA, the decellularized cartilage/GelMA composite scaffolds had lower swelling ratio, stronger mechanical strength. Bone marrow mesenchymal stem cells could adhere to scaffolds and proliferate. Conclusion: The scaffolds prepared by injectable decellularized cartilage/GelMA composite have low immunogenicity, suitable storage modulus, good biocompatibility, and are suitable for repairing cartilage defects. 2019 Vol. 49 (8): 581-588 [Abstract] ( 727 ) HTML (1 KB)  PDF (1752 KB)  ( 1004 ) 589 The effect of dexmedetomidine on inflammatory reaction and expression of related cytokines in lung tissues of septic mice LU Guangtao, LIN Xuezheng, YAO Zhangquan, WANG Junlu DOI: 10.3969/j.issn.2095-9400.2019.08.008 Objective: To explore the effect of dexmedetomidine (DEX) on inflammatory reaction and expression of related factors in lung tissues of septic mice stimulated with lipopolysaccharide (LPS). Methods: Male adult C57BL/6 mice were randomly divided into four groups: Control group (group C), Sepsis group (group S), DEX group (group MD) and High dose DEX group (group HD). Group MD and HD were respectively injected DEX solution 25 and 50 μg/kg through tail vein 30 min before modeling, while group C and S were injected equal amount of physiological saline. Septic mice model were then induced by tail vein injection of LPS. Six hours after LPS injection, the protein level of inflammatory cytokines in serum of mice were measured by ELISA kits; Western blot was performed to detect the activity of NF-κB (p65 and IκBα) and AKT. Lung tissues were collected to detect the wet/dry weight ratio (W/D), total lung water content (TLW) and degree of lung tissue injury. Septic model were establised in the same way, mice were monitored every 24 hours and recorded the survival rate for 7 days. Results: Compared with group C, there was a significant elevation in the protein level of TNF-α, CXCL1, CXCL5 and CCL20 (P<0.01) in group S, while DEX can inhibit the level of above-mentioned cytokines. Compared with group S, the pretreatment with DEX can significantly reduce the phosphorylation level of p65, IκBα and AKT (P<0.05), W/D ratio and TLW (P<0.05), and the degree of lung tissue injury. In addition, survival studies indicated that DEX significantly prolonged the survival time of septic mice (P<0.05). Conclusion: Pretreatment with DEX may protect lung tissues in septic mice by reducing the expression of AKT and NF-κB, and inhibiting the expression of inflammatory cytokines. 2019 Vol. 49 (8): 589-593 [Abstract] ( 741 ) HTML (1 KB)  PDF (1525 KB)  ( 812 ) 594 Clinical efficacy of sorafenib on patients with BCLC B/C stage hepatocellular carcinoma treated with transcatheter arterial chemoembolization CHENG Shimiao, LUO Songmei, LI Li, LYU Xiuling, WU Xulu, WENG Qiaoyou, CHEN Minjiang, YANG Hongyuan, JI Jiansong DOI: 10.3969/j.issn.2095-9400.2019.08.009 Objective: To explore the synergistic effect of the sorafenib on the efficacy of BCLC B/C hepatocellular carcinoma (HCC) patients treated with transcatheter arterial chemoembolization (TACE). Methods: Eighty-four patients with BCLC stage B/C hepatocellular carcinoma who were treated with TACE in our hospital from January 2013 to December 2017 were retrospectively selected. All patients were confirmed as hepatocellular carcinoma by biopsy, and further divided into TACE group (45 patients) and the sorafenib combined with TACE group (combination group, 39 patients) according to the presence or absence of sorafenib. All enrolled patients were followed up for three years, and clinical data were recorded. The disease control and survival benefits between the two groups were compared, and the synergistic effect of sorafenib on TACE was determined. Results: The tumor response of the combination group (64.1%) was significantly better than that of the TACE group (37.21%) (P=0.008). The 6-month, 1-year, 2-year and 3-year survival rates of patients in the TACE group were 60%, 40%, 13.3% and 8.9%, respectively, and those in the combination group were 82.1%, 51.3%, 30.8% and 16.9%. The overall survival time of the TACE group and the combination group was 12.9 months and 19.3 months, respectively; the progression-free survival time was 6.044 months and 16.218 months, respectively. The overall survival time (P=0.032) and progression-free survival time (P=0.006) of the combination group were significantly better than that of TACE group. Conclusion: For BCLC B/C stage HCC patients treated with TACE, sorafenib could significantly benefit the overall survival of patients and improve the TACE efficacy of HCC. 2019 Vol. 49 (8): 594-598 [Abstract] ( 777 ) HTML (1 KB)  PDF (2027 KB)  ( 728 ) 599 Application value of Z-DOM technique combined with iDose4 in CT scan of the upper cervical vertebrae of children CHEN Jiuzun, WU Aiqin, ZHENG Wenlong DOI: 10.3969/j.issn.2095-9400.2019.08.010 Objective: To investigate the application value of Z-DOM technique combined with iDose4 in reducing radiation dose in CT scan of the upper cervical vertebrae in children. Methods: A total of 55 child patients experiencing upper cervical vertebrae CT scan were selected as the experimental group, in which Z-DOM technique combined with iDose4 was used for low-dose scanning. Meanwhile, another 42 child patients receiving routine-dose CT scanning of the same site were selected as control group (not using Z-DOM technique combined with iDose4, 250 mAs). During the scanning, Volume CT dose index (CTDIvol) and Dose length product (DLP) of the two groups automatically displayed in the machine were respectively recorded, and the effective dose (ED) were calculated. The minimum and maximum values of mAs on the scanned image of each patient in the experimental group were also recorded. The image signal intensity, image noise, and background noise of each case were measured, and the signal noise ratio (SNR) was calculated. Results: The minimum mAs value of the experimental group was (45±11), and the maximum was (61±14), while the mAs values in the control group were all 250.0. Compared with the control group, the mAs values in the experimental group decreased by 234 mAs at maximum, with an average decline of 205 to 189 (75.6% to 82.0%). The CTDIvol, DLP, and ED of the experimental group were (3.7±1.1) mGy, (28.3±9.0) mGy•cm, and (0.3±0.1) mSv, respectively, while those in the control group were 169.0 mGy, (126.6±17.4) mGy•cm, and (1.4±0.2) mSv, respectively. The minimum mAs, the maximum mAs, the CTDIvol, the DLP and the ED were statistically different between the two groups. The intensity of the image signal, image noise, and background noise in the experimental group was (53.9±8.8), (7.1±1.8), and (8.5±1.4), respectively, while those in the control group were (57.2±5.8), (4.1±1.1), and (3.9±0.7), respectively. The SNR of the experimental group was (8.1±2.1) and that of the control group was (14.4±3.7). The intensity of image signal, image noise, background noise and SNR were statistically different between the experimental group and the control group. The image signal intensity and SNR of the experimental group were lower than those of the control group, while the image noise and background noise were higher. The diagnostic results of 28 cases of CT reexamination, 18 cases of MRI scans and 1 surgical case were consistent with those of the scan of the experimental group. Conclusion: With Z-DOM technology combined with iDose4, CT examination of the upper cervical vertebrae in children is of promising clinical appilcation value, as it enables real-time online adjustment of mAs and reduces the noise effectively, which cut down on radiation dose and ensures image quality. 2019 Vol. 49 (8): 599-602 [Abstract] ( 775 ) HTML (1 KB)  PDF (1258 KB)  ( 723 ) 603 Anti-compression and anti-rotation ability of dynamic hip screw-blade fixation for femoral neck fracture WU Jun, YANG Chengwei, ZHU Liulong, CHEN Liang DOI: 10.3969/j.issn.2095-9400.2019.08.011 Objective: To compare the anti-compression and anti-rotation ability of dynamic hip screw-blade (DHS-B) fixation in the treatment of femoral neck fractures. Methods: 8 femoral neck fracture specimens from 4 adult cadavers were prepared and divided into 2 groups. The observation group was treated with DHS-B fixation, while the control group was treated with 3 cannulated compression screws (CCS) fixation. Axial compression test, horizontal torsion test and axial compression test were adopted to evaluate the anti-compression and anti-rotation ability of the 2 stationary schemes. Results: With 500N axial load, the axial and medial strain of the femoral head in the observation group was significantly lower than those in the control group (P<0.05), there was no significant difference between the 2 groupsin the displacement of the femoral head (P>0.05). With 6 degrees torsion angle, there was no significant difference in torque between the 2 groups (P>0.05). The maximum axis carrying capacity of the observation group was higher than that of the control group (P<0.05). Conclusion: DHS-B has reliable ability of anti-rotation and anti-compression. It is suitable for the treatment of femoral neck fracture. 2019 Vol. 49 (8): 603-605，611 [Abstract] ( 690 ) HTML (1 KB)  PDF (1214 KB)  ( 817 ) 606 Clinical analysis of β-ketothiolase deficiency caused by ACTA-I gene compound heterozygous mutations GAO Yufeng, SHAN Xiao’ou DOI: 10.3969/j.issn.2095-9400.2019.08.012 Objective: To discuss the clinical manifestation of β-ketothiolase deficiency for early diagnosis so as to guide clinical practice. Methods: A case of β-ketothiolase deficiency diagnosed by gene sequencing in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University was reviewed. The data of clinical manifestation, laboratory examination and treatment were collected and summarized. Literature review on the treatment of β-ketothiolase deficiency was done. Results: A 4-year-old boy was admitted to our hospital with a second severe ketoacidosis attack. The first attack occurred at the age of 1 year and 10 months. The blood gas analysis indicated severe metabolic acidosis with normal blood ammonia, lactic acid, lipid profiles and uric acid. The urinary ketone was determined to be (++++), the level of 2 methyl 3 hydroxyisobutyric acid was also increased. In the second attack, the patient presented hypoglycemia with decreased blood glucose level at 2.6 mmol/L, while blood olhydroxybutyric acid was significantly increased, and blood free fatty acid was at normal high value. Urine ketone was determined to be (++). Blood tandem mass spectrometry showed a slight increase of 3-hydroxybutyrate carnitine, and urine gas mass spectrometry showed an increase of 3-hydroxybutyric acid. The result of gene sequencing revealed a compound, heterozygous mutation in ACAT-I (c.370A>C, c. 473A>G) of this patient, thusβ-ketothiolase deficiency was ultimately confirmed. Conclusion: It is necessary to consider the possibility of β-ketothiolase deficiency in infants with severe inexplicable ketoacidosis that happens after fasting, respiratory tract infection or gastroenteritis. When there are no specific abnormalities in blood and urine tandem mass spectrometry, ACTA-I gene detection is needed for further confirmation. 2019 Vol. 49 (8): 606-611 [Abstract] ( 769 ) HTML (1 KB)  PDF (1418 KB)  ( 1006 ) 614 The influence of ESPCS intervention on chronic heart failure in cardiac rehabilitation YE Lingxiao, WANG Lili, WANG Yanmin, ZHANG Liping DOI: 10.3969/j.issn.2095-9400.2019.08.014 Objective: To explore the effect of ESPCS (explain-simulate-practice-communication-support) on the cardiac rehabilitation of patients with chronic heart failure (CHF) through the intervention of ESPCS model. Methods: There were totally 152 CHF selected from the hospital between Oct. 2017 and Nov. 2018, who were divided as the observation group and the control group with 76 cases in each. The control group received routine nursing guidance, while health education plan was customized for CHF in the observation group by establishing ESPCS model. The heart failure knowledge test (HFKT) and the self-care of heart failure index (SCHFI), as well as the situation of the left ventricular ejective fraction (LVEF), left ventricular diastolic diamein (LVEDd) and the rate of re-hospitalization were compared. Results: Low scores of HFKT and SCHIFI in patients with CHF were observed. Before the implementation, there was no significant difference between LVEF and LVEDd. After the implementation, the HFKT scores and SCHFI scores in two groups were investigated respectively twice in a month after discharge. The scores of the observation group were significantly higher than the control group (P<0.05). The number of CHF hospitalized for heart-related reasons was significantly lower in the observation group (1 patient) than that in the control group (10 patients). Meanwhile the LVEF and LVEDd was significantly improved compared with that in the control group one month after discharge. Conclusion: ESPCS model can support the health education of patients’ cardiac rehabilitation, so that patients with CHF better understand discharge guidance and achieve more effective self care by learning relevant knowledge. 2019 Vol. 49 (8): 614-618 [Abstract] ( 826 ) HTML (1 KB)  PDF (1206 KB)  ( 1145 ) 619 The effect of innovative group activity on the ipmrovement of novice nurses’ innovative behavior ZHU Xueyan, XU Ke, WANG Juan, HE Lu, XIA Shuqi, HUANG Peipei DOI: 10.3969/j.issn.2095-9400.2019.08.015 Objective: To establish a model of innovation group activities and examine its effect on improving innovation behavior of novice nurses. Methods: A total of one hundred novice nurses in Wenzhou People’s Hospital were recruited. They had eight class hours of intensive training on innovation-related topics and then were divided into ten innovation groups to attend eleven meetings with innovation brain storm. Innovation atmosphere of the hospital and innovation behavior of the nurses were compared before and one year after the implementation of the model and various indicator changes were analyzed. Results: One year after the model, the total score of innovative atmosphere in the organization increased from (71.86±5.93) to (88.50±7.64). The total score of innovative behavior of the nurses was (35.03±4.36), significantly higher than that before the training (26.34± 2.38). The score of support dimension and realization idea were increased from (8.85±1.17) and (7.52±1.24) to (11.38±1.52) and (10.02±1.61) respectively, with significant difference (P<0.01). Conclusion: The innovative group activity model is beneficial to improve the innovative atmosphere of new nurses and improve their innovative behaviors. 2019 Vol. 49 (8): 619-621 [Abstract] ( 867 ) HTML (1 KB)  PDF (1159 KB)  ( 755 )

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