Home  |  About Journal  |  Editorial Board  |  Instruction  |  Subscribe  |  Messages Board  |  Contact Us  |  中文

Forthcoming Articles Current Issue Archive Advanced Search Read Articles Download Articles Email Alert

2019 Vol. 49, No. 7 Published: 2019-07-25   469 The expression of cathelicidin antimicrobial peptide increased by Protectin DX via P38 MAPK pathway in sepsis-induced ARDS GU Lijun, PAN Jingyi, Mohamed Ali Abdullahi, JIN Shengwei Objective: To explore the effect of PDX on the expression of cathelicidin antimicrobial peptide in sepsis-induced ARDS and the possible signaling pathway. Methods: C57BL/6 mice underwent cecal ligation and puncture to establish the sepsis model. Mice were divided as: Sham group, CLP group, CLP+PDX group, CLP+PDX+SB203580 group, CLP+PDX+DMSO group and PDX group. HE staining was used to evaluate the lung injury situation, CFU was used to detect the colony forming situation. The expression of cathelicidin antimicrobial peptide gene in lung tissue was detected by qPCR. Western blot was used to detect the expression of CAMP and the activation of P38 MAPK and ERK MAPK pathway. Results: Compared with Sham group, ERK MAPK pathway showed no significant change in the CLP group, while P38 MAPK pathway was significantly inhibited (P<0.05), and CAMP expression increased distinctly (P<0.05); compared with the CLP group, mice in the CLP+PDX group had less hemorrhage, inflammtory cell infiltration in lung tissue, decreased obviously CFU while the activation of P38 MAPK and the expression of cathelicidin antimicrobial peptide increased significantly (P<0.05). Conclusion: PDX increased the expression of cathelicidin antimicrobial peptide via P38 MAPK pathway in sepsis-induced ARDS. 2019 Vol. 49 (7): 469-474 [Abstract] ( 589 ) HTML (1 KB)  PDF (1694 KB)  ( 623 ) 475 Phenotypic analysis on Leber hereditary optic neuropathy pedigrees of only female-onset LYU Yuanyuan, XU Man, ZHANG Juanjuan, GUAN Minxin Objective: Phenotypic performance of 11 Leber’s hereditary optic neuropathy (LHON) pedigrees carrying m.11778G>A mutations, who had only female-onset, was analyzed. Methods: In this study we systematically screened, the MT-ND4 gene of 1 362 patients of the Chinese Han nationality with LHON. Mitochondrial genome sequencing and mitochondrial haplotype analysis were performed on 11 pedigrees carrying MT-ND4 m.11778G>A mutation. Results: Screening revealed that 11 cases of LHON pedigrees carrying m.11778G>A mutation only affected female maternal members. The LHON penetrance rate of these pedigrees showed low level, with 16.7%, 18.8%, 15.0%, 6.7%, 6.7%, 33.3%, 20.0%, 16.7%, 7.7%, 8.3%, 25.0% respectively. The average penetrance rate of each pedigree was 15.90%±0.08%. The degree of visual impairment in patients with LHON varied from mild to severe. The age of onset ranged from 7 to 25 years old. The average age of onset was (12.4±4.8) years old, being the high-risk age of LHON. The mitochondrial haplotype of 11 proband were B5a, Z, N9, B4c1b, G2h, B5a, M7b, N9a10, C7b, M7b1, M10 respectively. Conclusion: In this article, there were significant differences in LHON penetrance and age of onset between 11 different pedigrees with the same m.11778G>A mutation and different maternal members in the same mitochondrial genetic background. This suggests that m.11778G>A mutation is the molecular basis of the pathogenesis of LHON, but the mutation itself is not sufficient to induce the phenotypic expression of LHON, suggesting that other modification factors (mitochondrial haplotypes, nuclear modifier genes and environmental factors) may play a role in the development of LHON. 2019 Vol. 49 (7): 475-481,496 [Abstract] ( 741 ) HTML (1 KB)  PDF (1477 KB)  ( 765 ) 482 The efficacy of intravitreal anti-VEGF injection for retinal neovascularization of diabetic retinopathy HUANG Ying, SUN Zuhua, ZHOU Rong, LI Yingzi, LIU Xiaoling, LIN Bing Objective: To investigate the efficacy of anti-vascular endothelial growth factor (anti-VEGF) intravitreal injection for retinal neovascularization in diabetic retinopathy (DR) patients. Methods: In this retrospective study, DR patients who took fundus fluorescein angiography (FFA) examination from January 1, 2016 to April 28, 2018 at the Affiliated Eye Hospital of Wenzhou Medical University were reviewed. Inclusion criteria were as follows: ①patients with retinal neovascularization based on baseline FFA; ②Patients who only accepted anti-VEGF treatment, at least once; ③Patients who took FFA examinations again after all anti-VEGF treatments within 3 months. For the sake of analysis, the interval between the first and last anti-VEGF treatment was recorded as the duration of anti-VEGF treatment (it was zero when only one anti-VEGF treatment was given). And ‘untreatment period’ was the time between the last anti-VEGF treatment and the following FFA. Results: 20 patients (10 males and 10 females), 24 eyes were studied, with the average age being (53.9±12.8) years old. The mean number of anti-VEGF treatments was 2.5(1, 3), and the median total duration of anti-VEGF treatments was 62.0 (0, 76.8) days. The median untreatment period was 48.5 (30.0, 64.8) days. Through FFA, 15 eyes (62.5%) recovered from retinal neovascularization completely after anti-VEGF treatment, whereas the other 9 eyes (37.5%) recovered from retinal neovascularization partly and without new retinal neovascularization. The prolongation of untreatment period was a risk factor for neovascularization (OR=1.089, 95%CI=1.018-1.166, P=0.014). Four eyes had the median prolonged un-treatment period of 177.0 (90.5, 234.3) days, and recurrence of neovascularization was observed. Conclusion: Intravitreal injection of anti-VEGF is effective in control of retinal neovascularization of DR. Retinal neovascularization may recur, and thus repeated treatment is required. 2019 Vol. 49 (7): 482-486 [Abstract] ( 614 ) HTML (1 KB)  PDF (1402 KB)  ( 726 ) 487 Risk factors of diabetic retinopathy LIN Sigeng, DENG Yuxuan, WEN Liang, LIANG Yuanbo, QU Jia Objective: To investigate the risk factors of diabetic retinopathy (DR). Methods: The current study was conducted in patients with type 2 diabetes mellitus (DM) by means of handy sampling from July to October 2018 in one community of Yueqing County, Wenzhou, China. Face-to-face interview, routine laboratory tests and eye examinations were applied by professional investigators strictly following by the specific standard operation procedures (SOP) of the present study. Multivariable logistic regression models were performed to screen the potential influential factors of DR. Results: Among 406 type 2 DM patients aged 40 years and over, 177 (43.6%) were diagnosed as DR (107 with mild DR, 57 with moderate DR, and 13 with severe DR). As compared with their counterparts, the incidence rate of DR in participants with the duration of DM from 5 to 9 years would like to be significantly increased by 8% [OR=1.08, 95%CI=0.69-1.67, P=0.008]. Meanwhile, patients whose glycosylated hemoglobin A1c (HbA1c) over 6.5% would have additional 16% chance to develop DR (OR=1.16, 95%CI=0.65-2.06, P=0.002) compared with those with HbA1c less than 6.5%. Conclusion: DR screening should be conducted in type 2 DM patients, especially in those with type 2 DM for more than 5 years and with abnormal HbA1c. 2019 Vol. 49 (7): 487-490 [Abstract] ( 746 ) HTML (1 KB)  PDF (1234 KB)  ( 789 ) 491 Repeatability analysis of different mathematical models of magnetic resonance diffusion weighted imaging in rat brain LU Yi, CHEN Tao, PENG Wenwen, SONG Jiao, WANG Xue, YAN Zhihan Objective: To evaluate the repeatability of different magnetic resonance diffusion weighted imaging (DWI) mathematical modelsin the brains of rats, such as the apparent diffusion coefficient (ADC) model, intravoxel incoherent motion (IVIM) model, diffusion kurtosis imaging (DKI) model and stretched exponential model. Methods: There were 14 male Sprague-Dawley rats experienced DWI scanning in 3.0T scanner twice, in 24-hours intervals. The data was analyzed with different diffusion mathematical models. The intra-subject coefficient of variation (intra-subject CV) and Bland-Altman analysis were used to evaluate the repeatability of the experiment. Results: In the ADC model, DKI model and stretched exponential model, the Cronbach’s alpha of these diffusion related parameters (ADC, Dapp and DDC) were less than 5%, intra-subject CV were less than 0.7, and LA were less than 0.15×10-3 mm2/s. Moreover, ADC had the smallest of LA, which was 0.086×10-3 mm2/s. Cronbach’s alpha of all the coefficients of the IVIM model were less than 0.5, intra-subject CV were more than 40%, and LA were greater than 0.4×10-3 mm2/s. Among different regions of Interest, minimum intra-subject CV and LA of ADC were in striatum, respectively: 2.00% and 0.034×10-3 mm2/s. And Corpus callosum had the highest intra-subject CV and LA of ADC, respectively 3.98% and 0.085×10-3 mm2/s. Conclusion: The repetitive analysis results of different diffusion mathematical models, which from the DWI of rat’s brain in a intervals of 24 hours, are significantly different. The repeatability of ADC model is the best, and IVIM model is the worst. In addition, the repeatability of ADC value in different brain ROIs of rats are also significantly different, which shows that striatum has a best repeatability, and corpus callosum is relatively poor. 2019 Vol. 49 (7): 491-496 [Abstract] ( 706 ) HTML (1 KB)  PDF (1500 KB)  ( 702 ) 497 Grey matter volume changes in patients of mild traumatic brain injury: a voxel-based morphometry study YANG Dengfa, JIN Hanghuang, ZHANG Sujuan, YANG Ting, WU Liang, WANG Linyou, SHEN Jianmin, YU Xinfeng Objective: To evaluate the volume changes of grey matter and its clinical significance in patients of mild traumatic brain injury with a voxel-based morphometry method. Methods: Whole brain structural images with FFE T1WI were acquired in 24 mTBI patients and 24 mTBI gender-and age-matched healthy controls, SPM8 was used to preprocess the collected data, and VBM software was used to analyze the whole brain structure. The difference of gray matter volume between the two groups was compared by double sample t test. Results: Decreased gray matter volumes were found in some regions including left cingulate gyrus, right cingulate gyrus, left shell nucleus, and right shell nucleusn (all P<0.05). Increased gray matter volumes were found in some regions including upper right frontal gyrus and middle left frontal gyrus. Clinical symptoms included neurologic symptoms, cognitive function and behavior disorders. There were headache and dizziness in 19 cases, memory decline in 12 cases, rage in 9 cases, inattention in 8 cases, unflexible body in 6 cases, two or more symptoms appeared in 15 cases at the same time. Conclusion: Changes of gray matter volume were demonstrated in mild traumatic brain injury patients with voxel-based morphometry method, which may be related to its clinical symptoms. 2019 Vol. 49 (7): 497-500 [Abstract] ( 678 ) HTML (1 KB)  PDF (1279 KB)  ( 692 ) 501 Supine and prone flexion MRI in the dynamic evaluation of the association between morphological changes of lumbar multifidus muscle and lumbar disc herniation SU Na, LIU Qi, YAN Chunli, LIU Miao, LU Hongjiang, CHEN Xingcan Objective: To evaluate the value of the difference in lumbar multifidus muscle (LMM) morphology for lumbar disc herniation (LDH) by dynamic evaluation with supine and prone flexion MRI. Methods: A total of 43 patients with LDH and 42 normal control (NC) subjects at 903 RD Hospital of PLA from March 2016 to April 2018 were enrolled in this study. All patients and healthy volunteers underwent supine and prone flexion paired lumbar MRI, including sagittal T1WI, T2WI and axial T2WI sequences. LMM morphological parameters were obtained using the ImageJ software, including the total cross-sectional area (TCSA), the functional cross-sectional area (FCSA), muscle cross-sectional area asymmetry (CSAasy), fatty infiltration ratio (FIR), and muscle morphological change between supine and prone flexion positions (S-P). The ROC curve was used to evaluate the differentiating ability of multifidus morphological parameters on LDH. Results: In both LDH and NC groups, TCSA of supine position was larger than that of prone flexion position in L3-S1 segments (P<0.001). At L3-4 segment, S-P was lower in LDH group than NC group (P<0.001); At L4-5 segment, TCSA and S-P were lower in LDH group than NC group (P<0.05), while CSAasy was higher in LDH group than NC group (P=0.015). At L5-S1 segment, TCSA, FCSA and S-P were lower in LDH group than NC group (P<0.05). The results of ROC curve showed that the classification performance of S-P (AUC=0.957) was better than other LMM morphological parameters (TCSA: AUC=0.723; FCSA: AUC=0.704; CSAasy: AUC=0.662; FIR: AUC=0.643). Conclusion: The supine and prone flexion MRI could be a useful tool to dynamically evaluate LMM morphological changes. Declined of LMM morphological change was demonstrated in LDH patients, of which contractive impairment of LMM may be an important factor. 2019 Vol. 49 (7): 501-506 [Abstract] ( 637 ) HTML (1 KB)  PDF (1342 KB)  ( 723 ) 507 The value of ultrasound assessment of fetal nasal bone dysplasia in the diagnosis of fetal chromosomal abnormalities during the second and the third trimesters of pregnancy Qian Xiaoting, Bao Lingyun, Huang Anqian, Xu Yanjun, Shi Junhua, Huang Yayuan, Kan Guangjuan, Wang Hongyang Objective: To investigate the value of ultrasound assessment of nasal bone development abnormalities in the diagnosis of chromosomal abnormalities during the second and the third trimester. Methods: A retrospective analysis was made of 63 cases of nasal dysplasia during the second and the third trimester of ultrasound from January 2013 to March 2018 in the First People’s Hospital of Hangzhou. The cases were divided as simple nasal dysplasia group (16 cases) and nasal dysplasia with other structural abnormalities (47 cases), depending on whether they were combined with other ultrasonic soft indexes or structural abnormalities. Ultrasound results were compared with chromosomal analysis results and (or) single nucleotide polymorphism microarray analysis results. All these cases were followed up to fetal induction or 3 months after birth. Results: Chromosomal analysis was performed in 44 out of 63 cases. A total of 20 chromosomal abnormalities were detected, including 14 cases (70%) of 21-trisomy, 1 case (5%) of 18-trisomy in, 2 cases (10%) of 13-trisomy, and 3 cases (15%) of other abnormalities. The incidence of chromosomal abnormalities in the group of nasal dysplasia with other soft ultrasound or structural abnormalities was found to be higher than that of simple nasal dysplasia group (62.1% vs. 13.3%) (χ2=9.47, P<0.05). Conclusion: The development of fetal nasal dysplasia during the second and the third trimester is closely related to chromosomal abnormalities. In particular, fetuses with other ultrasonic soft indexes or structural abnormalities need to further determine the karyotype and/or single nucleotide polymorphism microarray analysis. Ultrasound assessment of nasal development should serve as an important indicator. 2019 Vol. 49 (7): 507-511 [Abstract] ( 712 ) HTML (1 KB)  PDF (1330 KB)  ( 978 ) 512 The value of serum amyloid A in the early clinical diagnosis of Vogt-Koyanagi-Harada syndrome YANG Yuanyuan, LI Yali, WANG Daxuan, CHEN Pengfei, ZHENG Meiqin Objective: To investigate the clinical value of serum amyloid A(SAA), c-reactive protein (CRP) and white blood cell count (WBC) in early diagnosis of Vogt-Koyanagi-Harada syndrome. Methods: Ninety-two patients with Vogt-Koyanagi-Harada syndrome admitted to the Eye Hospital of Wenzhou Medical University from June 2016 to March 2018 were selected as the study subjects; 78 patients with dry eye disease were selected as the eye disease control group and 95 normal people with gender and age matching as the normal control group. SAA and CRP levels of the three groups were detected by colloidal gold immunochromatography and immunofluorescence dry quantitative method respectively; WBC was detected by Sysmex XT-1800i automatic blood analyzer. Variables were compared using Kruskal-wallis test and diagnostic value was measured by using ROC curve. Results: SAA, CRP and WBC levels were significantly increased in the Vogt-Koyanagi-Harada syndrome patients group compared with the dry eye group and the control group, respectively, with statistical difference (P<0.05). Compared with the control group, the differences were not statistically significant in the dry eye group (P>0.05). SAA had positive correlation with CRP and WBC in Vogt-Koyanagi-Harada syndrome patients (all P<0.05). The levels of SAA and WBC were significantly lower after treatment than before treatment (all P<0.05), the levels of CRP were not significantly different before and after the treatment (P>0.05). According to ROC curve analysis, the auC of SAA, CRP, WBC and combined detection for diagnosis of Vogt-Koyanagi-Harada syndrome were 0.905, 0.565, 0.888 and 0.952, respectively. The optimal cut-off values were 3.31 mg/L, 5.55 mg/L, and 8.175×109/L, respectively. The optimal cut-off value corresponding to the maximum value of Youden index was 0.667 by combined detection, with the sensitivity being 78.26% and the specificity 97.89%. Conclusion: SAA combined with CRP and WBC could improve the diagnostic efficacy of Vogt-Koyanagi-Harada syndrome. SAA can provide useful reference for the auxiliary diagnosis of Vogt-Koyanagi-Harada syndrome, which is worthy of wide clinical application. 2019 Vol. 49 (7): 512-516 [Abstract] ( 605 ) HTML (1 KB)  PDF (1287 KB)  ( 907 ) 517 Association between the expression level of placental plasma membrane fatty acid binding protein and non-gestational diabetes mellitus macrosomia HAN Ying, WANG Chenchen, WANG Yuhuan, YE Yan, YAN Hongtao, YANG Xinjun Objective: To investigate the association between the expression level of placental plasma membrane fatty acid binding protein (FABPpm) and non-gestational diabetes mellitus (non-GDM) macrosomia.Methods: A case-control study was performed. Forty macrosomia newborns (birth weight≥4 000 g) with normal pregnancy and full-term delivery were collected as case group from June 2015 to June 2016 continuously in the obstetric department of the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University. And 40 normal-birth-weight newborns (birth weight ranging from 2 500 to 3 999 g) were randomly selected as control group from those delivered within 3 days of macrosomia delivery. Self-designed questionnaires were used to collect maternal and fetal information, the placenta and cord blood samples were obtained. We detected the placental mRNA and protein expression levels of FABPpm by real-time quantitative PCR and western blotting, and determined the non-esterified fatty acids levels in umbilical cord blood by colorimetry. Logistic regression analysis was used to analyze the influencing factors of macrosomia. Results: The expression levels of FABPpm mRNA and protein in the placenta of macrosomia group were higher than those of control group (P<0.05). The FABPpm mRNA expression in placenta was positively correlated with birth weight in both groups (control group: rs=0.337, P=0.033; macrosomia group: rs=0.493, P=0.002). And FABPpm mRNA expression was positively correlated with NEFA level of umbilical cord blood in the control group (rs=0.334, P=0.046), but not in the macrosomia group. Multivariate logistic regression analysis showed that the higher FABPpm expression in placenta could increase the risk of macrosomia (OR=3.138, 95%CI=1.050-9.382, P=0.041), and the occurrence of macrosomia was also influenced by the fetal gender (OR=3.229, 95%CI=1.002-10.403, P=0.049) and gestational weight gain (OR=3.573, 95%CI=1.018-12.543, P=0.047). Conclusion: The up-regulation of FABPpm expression in placenta may increase the risk of non-GDM macrosomia, suggesting that FABPpm may affect the occurrence of macrosomia by affecting fatty acid transport. 2019 Vol. 49 (7): 517-522 [Abstract] ( 567 ) HTML (1 KB)  PDF (1369 KB)  ( 707 ) 523 The effect of vildagliptin on neural cell apotosis and locomotor recovery in a rat model of spinal cord injury XU Tianzhen, WANG Chenjian, TANG Chengxuan Objective: To investigate the effect of Vildagliptin (Vilda) on locomotor function recovery and neural cell apotosis in a rat model of spinal cord injury. Methods: Forty-eight SD rats were randomly divided into 3 groups (n=16): the Sham group, the SCI group and the SCI+Vilad group. Clip compression SCI model was established as previously described. The SCI+Vilad group was treated with Vilad at a dose of 10 mg·kg-1·d-1 by gavage for 7 days in a row, then once every 3 days; the SCI group was treated with the same dosage of normal saline. The Basso-Beattie-Bresnahan (BBB) score was obtained at 1, 3, 7, 14, 21, 28 d after operation. Immunofluorescence staining and Western blot were used to detect the expression of apoptotic related proteins expression (Bcl-2, Bax, Cleaved Caspase 3) at 3 d after operation. Nissl staining was performed at 7 d after operation to detect the surviving neurons. At 28 d, the, the footprint analysis was used to assess the locomotor recovery. Results: Compared with the Sham group, SCI group showed significantly lower BBB scores and inconsistent footprints; the number of VMNs was decreased significantly. Immunofluorescence staining and western blot results showed enhanced expression of apoptotic related proteins (Bax, Cleaved Caspase 3), but the expression of anti-apoptotic related protein (Bcl-2) was decreased (P<0.05). Compared with the SCI group, the rats in SCI+Vilda group got higher BBB scores, more consistent footprints; the number of VMNs was also increased. At the same time, the expression of apoptotic related proteins (Bax, Cleaved Caspase 3) was decreased significantly and the expression of anti-apoptotic related protein (Bcl-2) was increased obviously (P<0.05). Conclusion: All of these findings indicated that Vildagliptin shows neuroprotective effect after SCI in rats via inhibiting the VMNs apoptosis, and finally promotes the locomotor recovery. 2019 Vol. 49 (7): 523-528 [Abstract] ( 617 ) HTML (1 KB)  PDF (1525 KB)  ( 809 ) 529 BC200 inhibits the temozolomide-induced apoptosis by mediating the maintenance of stemness of glioma cells TONG Minfeng, YANG Fan, LIU Jihong, XU Hu Objective: To investigate the role of serum long-chain non-coding RNA BC200 (lncRNA BC200) in glioma stem cells (GSCs) and related mechanism. Methods: Glioma U87 MG cells were cultured in neural stem cell culture medium and extracted into spherical growth GSCs. Immunofluorescence was used to detect the marker molecules CD133 and nestin of GSCs stem cells. Small RNA (siRNA) transfection silenced BC200 expression in GSCs. The GSCs were treated with Temozolomide (TMZ, 200 μmol·L-1) for 24 h, and the effect of TMZ on the morphology of suspended cells in GSCs was observed under light microscope. The expression of CD133, Bax, Bcl-2 and cleaved caspase-3 was detected by Western blot. Results: The extracted GSCs were isolated for spheroid growth, and the cell surface CD133 and nestin were positively expressed. After TMZ treatment of GSCs for 24 h, the ability of GSCs to form spheres did not change significantly, and the Bax, cleaved caspase-3 and Bcl-2 did not change significantly at 24 h. The expression of CD133 in GSCs was significantly decreased after silencing BC200. After BC200 silencing, TMZ treatment of GSCs showed that its ability to form a ball was significantly weakened, and the cells were dispersed. At 24 h, cleaved caspase-3 and Bax were up-regulated (45.36±4.25)% and (63.23±5.12)% (P<0.01). Bcl-2 was down-regulated (31.22±3.80)% (P<0.01). Conclusion: BC200 is able to maintain the dryness of glioma U87 MG cells, which promotes cell resistance to TMZ-induced apoptosis. 2019 Vol. 49 (7): 529-532,537 [Abstract] ( 633 ) HTML (1 KB)  PDF (1583 KB)  ( 694 ) 533 Changes of related indicators in severe preeclampsia complicating acute left heart failure WU Liqun, XU Qun, YAO Rui, GAO Jie, CAI Pingsheng Objective: To analyze changes of related indicators in patients with severe preeclampsia (SPE) complicated with acute left heart failure. Methods: A total of 72 cases of SPE were selected as subjects. They were assigned into two groups: the observation group (22 cases) with acute left heart failure and the control group (50 cases) without acute left heart failure. Meaningful indicators were analyzed by logistic regression analysis. Meanwhile, ROC curve was drawn to evaluate their diagnostic value. Results: ①The findings showed that the heart rate, respiratory rate, BNP, NLR, CRP, creatinine and troponin I in the observation group were higher than those in the control group; The oxygen saturation and LVEF in the observation group were lower than those in the control group (P<0.05); ②Logistic regression analysis showed that elevated levels of BNP, NLR, CRP and troponin I were independent risk factors for SPE complicated with acute left heart failure; [OR(95%CI): 1.01(0.89-1.62), 1.61(0.97-2.68), 1.27(1.06-1.51) and 5.65(1.46-21.95), all P<0.05]. ③When levels of BNP (NLR) CRP and troponin I higher than the cut-off point, BNP in maternal blood was of the greatest value in the diagnosis of SPE complicated with acute left heart failure among all single markers, followed by NLR, CRP and troponin I in maternal blood (The AUC were 0.712, 0.683, 0.658 and 0.657, respectively). When four indexes (BNP, NLR, CRP and troponin I) were used in combination, AUC was 0.732. Conclusion: The elevated levels in maternal blood of BNP, NLR, CRP, troponin I were associated with SPE complicated with acute left heart failure. The diagnostic value of four indexes combined was superior to that of a single index. 2019 Vol. 49 (7): 533-537 [Abstract] ( 726 ) HTML (1 KB)  PDF (1263 KB)  ( 785 ) 538 Chinese version of Neonatal/Infant Braden Q Scale in the assessment of neonatal pressure injury risk in NICU WU Yujie, LU Ping, LYU Junying, SONG Fei, ZHANG Shuanghong, ZHANG Liping Objective: To introduce and translate the US Neonatal/Infant Braden Q Scale (N/I Braden Q scale) into Chinese version, and test the reliability and validity of the scale. Methods: The N/I Braden Q scale was translated according to the Brislin translation model and cross-cultural adaptation using the Delphi technique. Then, the scale was applied to the NICU hospitalized newborns for skin condition assessment in one Grade A hospital of Zhejiang Province, and the reliability and validity of the scale in the Chinese context were tested. Results: Inter-rater reliability coefficient of the scale was 0.908 (P<0.001). The overall consistency of the scale was 0.75, the mean S-CVI was 0.9583 and the item-level content validity index was 0.83~1. The predicting cut-off point of the scale is score 20, showing a sensitivity of 75.00%, specificity of 74.16%, positive predictive value of 28.13%, negative predictive value of 95.62%, Yoden index of 0.4916 and receiver operating characteristic curve was 0.815 (95%CI: 0.684-0.945) (P<0.001). Conclusion: The Chinese version of N/I Braden Q scale has good reliability and validity, and has an early warning effect on the occurrence of neonatal PI. It can be used as a structured guidance tool for clinical nurses to conduct skin assessment on children. 2019 Vol. 49 (7): 538-541,544 [Abstract] ( 691 ) HTML (1 KB)  PDF (1284 KB)  ( 997 )

Copyright © Editorial Board of JOURNAL OF WEZHOU MEDICAL UNIVERSITY Supported by:Beijing Magtech