Home  |  About Journal  |  Editorial Board  |  Instruction  |  Subscribe  |  Messages Board  |  Contact Us  |  中文

Forthcoming Articles Current Issue Archive Advanced Search Read Articles Download Articles Email Alert

2019 Vol. 49, No. 1 Published: 2019-01-25   1 Catechol estrogen mediates chronic intermittent hypoxia induced OSAHS enuretic model in female rats XU Li, SU Miaoshang, CAI Xiaohong, LI Changchong DOI: 10.3969/j.issn.2095-9400.2019.01.001 Objective: To establish obstructive sleep apnea and hypopnea syndrome (OSAHS) enuretic model induced by chronic intermittent hypoxia (CIH) exposure in female rat, and to explore the role of catechol estrogen (CE) in CIH induced OSAHS enuretic model. Methods: Sixteen Sprague-Dawley (SD) female rats (6 weeks old) were randomly divided into CIH group and the control group, with eight rats for each group. The CIH group was exposed to chronic intermittent hypoxia cabin for 4 weeks; meanwhile, the control group was exposed to normal air cabin. Water intake and urine output were recorded; the levels of CE, NA, Adr and DA in serum and the activity of COMT were measured, and threshold pressure was measured; bladder was taken for HE and Weigert staining, then the histological changes under light microscope were observed. Results: Compared with the control group, the water intake and urine output outside the cabin in CIH group had no significant difference. However, there was significant increase of urine output inside cabin in CIH group. Furthermore, the levels of CE, NA, Adr and DA in serum were significantly higher, whereas the activity of COMT and the threshold pressure were significantly decreased compared with that in the control group. HE staining showed the disordered arrangement of bladder detrusor muscle bundle and some breaking muscle bundles. Conclusion: CIH induces enuresis in female rats accompanying with increased urine output and CE, NA, Adr and DA levels in serum, decreased activity of COMT and threshold pressure, which are similar to the clinical characteristics in enuresis children. It suggests that it is feasible to use CIH to establish OSAHS enuretic female rat model. 2019 Vol. 49 (1): 1-5 [Abstract] ( 926 ) HTML (1 KB)  PDF (1395 KB)  ( 770 ) 6 The effect of different strategies to prevent ovarian hyperstimulation syndrome in high-risk patients undergoing ovarian stimulation protocol in IVF TENG Yili, YU Rong, XU Zhihui, PAN Jiexue, YANG Haiyan, HUANG Xuefeng DOI: 10.3969/j.issn.2095-9400.2019.01.002 Objective: To investigate the effectiveness of three strategies to prevent ovarian hyperstimulation syndrome (OHSS) in high risk patients undergoing IVF cycles. Methods: Patients in group A (n=95) received decreased dose of HCG trigger. Patients in group B (n=23) were subjected to transvaginal aspiration of small follicles. Patients in group C (n=60) were subjected to in-vitro maturation (IVM). Results: No significant differences were detected in the rate of high-quality embryos, clinical pregnancy rate per-transfer and live birth delivery rate per transfer among 3 groups (P>0.05). No severe OHSS was observed in all patients. The incidence of mild-moderate OHSS was significantly higher in group A (50.53%) and B (34.78%), while none was observed in group C (0%). Conclusion: Transformation into IVM resulted in completely absence of OHSS. Follicle aspiration and decreased HCG trigger could both avoid severe OHSS and remarkably reduce the occurrence of mild-moderate OHSS. OHSS high risk patients could have ideal pregnancy outcomes with the above-mentioned three strategies. 2019 Vol. 49 (1): 6-10 [Abstract] ( 835 ) HTML (1 KB)  PDF (1293 KB)  ( 970 ) 11 The inhibition of interfering SIRT7 in the proliferation, migration and invasion of thyroid cancer cell Chen Xiaoxi, Chen Xuxu, Lin Jiahao, Chen Jicai, Huang Guoyu DOI: 10.3969/j.issn.2095-9400.2019.01.003 Objective: To study the effect of interfering SIRT7 on the proliferation, migration and invasion of thyroid cancer cell BCPAP in vitro. Methods: The stable strain of BCPAP interferred with SIRT7 was constructed by lentivirus, and the interference effect was verified at mRNA and protein levels. The effects of SIRT7 on the proliferation, migration and invasion of thyroid cancer BCPAP in vitro were studied by cell migration and cell invasion assay. Results: The stable cell line of BCPAP interfered with SIRT7 was successfully constructed, and after interfering SIRT7, the proliferation activity of BCPAP, the ability of scar healing and the ability of cell migration and invasion were significantly decrease and showed statistical difference (P<0.05). Conclusion: SIRT7 might play a role as an oncogene in thyroid cancer and could be considered a potential therapeutic target against thyroid cancer. 2019 Vol. 49 (1): 11-14，23 [Abstract] ( 815 ) HTML (1 KB)  PDF (1418 KB)  ( 889 ) 15 Expression and significance of Th22 cells and IL-22 in the occurrence and development of primary hepatocellular carcinoma SHI Zhenjing, WU Daoyi, WU Lili, QIAO Binbin, ZHENG Bingru, LI Cheng, SHI Changsheng, YU Xixiang DOI: 10.3969/j.issn.2095-9400.2019.01.004 Objective: To study the expression and significance of Th22 cells and IL-22 in the development and progression of primary hepatocellular carcinoma. Methods: A total of 42 hospitalized patients with chronic hepatitis B cirrhosis (cirrhosis group), 38 hospitalized patients with hepatocellular carcinoma (hepatocellular carcinoma group), 30 healthy volunteers (healthy group) and 21 cases of chronic hepatitis B (CHB group) were randomly selected from the Third Affiliated Hospital of Wenzhou Medical University from January 2015 to December 2016. The differences in AST, ALT, TBIL and ALB values were analyzed. Blood was collected intravenously into 2 heparin anticoagulant tubes, one for testing IL-22 expression level and the other for determination of Th22 cell levels using flow cytometry. The statistics of Th22 and IL-22 levels were analyzed. Results: The AST, ALT, TBIL and ALB values in patients with cirrhosis and hepatocellular carcinoma were significantly higher than those in the CHB group and healthy group (P<0.01). The AST, ALT, TBIL and ALB values of patients in the hepatocellular carcinoma group were higher than those in cirrhosis group (P<0.01). There was no significant difference between CHB group and healthy group (P>0.05). In addition, the AST, ALT, TBIL and ALB gradually increased with the development of hepatocellular carcinoma (P<0.05). The levels of Th22 and IL-22 in liver cirrhosis group and hepatocellular carcinoma group were higher than those in healthy subjects (P<0.05). Th22 and IL-22 levels in hepatitis B liver cancer group were higher than those in hepatitis B cirrhosis group (P<0.05). The levels of Th22 and IL-22 were gradually increased with the development of hepatocellular carcinoma (P<0.05). Conclusion: Th22 and IL-22 may be related to the occurrence and development of HCC. 2019 Vol. 49 (1): 15-19 [Abstract] ( 868 ) HTML (1 KB)  PDF (1418 KB)  ( 804 ) 20 The preparation of hesperidin suppository and its quality evaluation JIN Hui, LIN Jiale, WANG Chenxiang, ZHOU Ziye DOI: 10.3969/j.issn.2095-9400.2019.01.005 Objective: To prepare hesperidin suppository and establish a quality control method. Methods: Hesperidin was used as an active ingredient. Polyethylene glycol 400, polyethylene glycol 6000 and glycerol were chosen as substrates. Fusion method was applied to prepare the hesperidin suppository. The content uniformity, appearance, melting time and weight variation were evaluated. Results: The hesperidin suppository showed brown appearance and desirable hardness at room temperature. The content uniformity, weight variation and melting time of hesperidin suppository prepared by this method met the requirements of quality control. Conclusion: The hesperidin suppository prepared by this method meet the quality requirements of suppository and may be developed as a new formulation of hesperidin. 2019 Vol. 49 (1): 20-23 [Abstract] ( 923 ) HTML (1 KB)  PDF (1308 KB)  ( 853 ) 24 The changes of leukoaraiosis and cognitive function after carotid stenosis and carotid artery stenting ZHOU Lin, SHEN Minhui, SUN Maojun, WANG Binda, TANG Weiguo DOI: 10.3969/j.issn.2095-9400.2019.01.006 Objective: To investigate the correlation between carotid artery stenosis and leukoaraiosis (LA) and cognitive impairment, and the changes of LA and cognitive function after carotid artery stenting (CAS). Methods: The study included 44 hospitalized patients with carotid artery stenosis greater than 70% which were confirmed by digital subtraction angiography from Zhoushan Hospital Affiliated to Wenzhou Medical University during August 2014 to August 2017. All the patients were divided into two groups, with 22 cases in both stenting group and the control group. The Cranial MRI examination was performed at the baseline and six-month follow-up. Before treatment, 1 month after treatment and 6 months after treatment, neuropsychological tests were performed, including Mini-Mental State Examination (MMSE) and Wechsler Adult Intelligence Scale-RC (WAIS-RC). The baseline characteristics and follow-up results were compared in each group. Results: After controlling other risk factors, the volume of LA lesions was still related to the degree of carotid artery stenosis (r=0.473, P<0.05), the score of MMSE was still related to the degree of carotid artery stenosis (r= -0.330, P<0.05). Compared with the control group, the volume of LA lesions and the score of MMSE were both significantly ameliorated in the stent implantation group after treatment (P<0.05). The score of WAIS-RC was significantly improved after operation compared with preoperative, mainly in the arithmetic, digit span, digit symbol, block design test. Conclusion: ①Carotid artery stenosis was related to LA and cognitive dysfunction. The more serious the stenosis is, the greater volume of LA lesions and the more severe of cognitive impairment. ②LA and cognitive function was ameliorated after CAS. 2019 Vol. 49 (1): 24-29 [Abstract] ( 770 ) HTML (1 KB)  PDF (1299 KB)  ( 908 ) 30 Genetic analysis of the pedigree with inherited coagulation factor XI deficiency caused by a homozygous Tyr503Cys mutation ZHOU Xingxing, LI Xiaolong, JIN Yanhui, YANG Lihong, PAN Jingye, SU Kankan, WANG Mingshan DOI: 10.3969/j.issn.2095-9400.2019.01.007 Objective: To detect the phenotype and gene of a hereditary factor XI (FXI)-deficient pedigree caused by a homozygous Tyr503Cys missense mutation in search for the causative gene and exploration of its molecular pathogenesis. Methods: Prothrombin time (PT), activated partial thromboplastin time (APTT), coagulation factor VIII activity (FVIII:C), coagulation factor IX activity (FIX:C), coagulation factor XI activity (FXI:C), coagulation factor XII activity (FXII:C), and FXI antigen (FXI:Ag) were assayed in probands and their family members (five persons in three generations) to confirm the diagnosis. All exons and flanking sequences of the F11 gene of the proband were analyzed by direct PCR sequencing. The mutation sites were confirmed by reverse sequencing and the corresponding mutation site regions of the familial members were detected. The conservation of amino acid mutation sites was analyzed using ClustalX-2.1-win software; the effect of mutations on protein function was analyzed using PolyPhen-2, PROVEAN, and Mutation Taster software; the mutation site was analyzed by Swiss-PdbViewer software for protein model and amino acid interactions. Results: The APTT of proband was 59.3 s, prolonged obviously, and the FIX:C decreased to 13%; the APTT of mother, daughter and son were all prolonged to varying degrees, the FIX:C fell to 37%-42%, and the family of five people’s FXI:Ag were in the reference range. Genetic analysis revealed that there was a c.1562A>G homozygous missense mutation in exon 13 of the F11 gene in the proband, leading to Tyr503Cys; heterozygotes of the Tyr503Cys mutation existed in the mother, daughter, and son. Conservative analysis showed that Tyr503 is highly conserved among homologous species. The three bioinformatics software’s predictive results for this mutation were consistent: both predicted that this mutation was likely to be a deleterious mutation that could cause related diseases. Mutant protein model analysis showed that wild-type Tyr503 forms two hydrogen bonds with Ile370 and Lys554; a hydrogen bond was added between mutant Cys503 and Lys554. Conclusion: There was a c.1562A>G homozygous missense mutation in exon 13 of the F11 gene in the proband, laeding to Tyr503Cys. It was speculated that the homozygous mutation was inherited from a parent with a heterozygous Tyr503Cys homozygote and that it was associated with reduced FXI levels in the family. 2019 Vol. 49 (1): 30-33，37 [Abstract] ( 957 ) HTML (1 KB)  PDF (1450 KB)  ( 1241 ) 34 The value of ultrasonic characteristics of the thyroid nodule calcification in the diagnosis of thyroid cancer XU Fangting, SUN Yang, LUO Jieli, HUANG Pintong DOI: 10.3969/j.issn.2095-9400.2019.01.008 Objective: To evaluate the diagnostic value of the calcification characteristics in the thyroid nodule including its size, location, quantity, and acoustic shadow. Methods: We reviewed sonographic findings of all calcified nodules which underwent ultrasound-guided fine needle aspiration (FNA) or surgery between July 2015 and April 2017. Surgical pathology or FNA cytology combined with follow-up result was regarded as the ‘Reference Test’. All nodules were firstly divided into three groups according to the size of calcification: ≤1 mm, 1~2 mm, >2 mm, and then the definition of macro-calcification (>1 mm or 2 mm) was determined by analysing the malignancy rate of each group. Secondly, sonographic characteristics of the macro-calcification including its location, quantity and acoustic shadow were analyzed. Results: Among suspiciously malignant nodules on ultrasound, the malignancy rate of nodule with calcification ≤1 mm was significantly higher than nodule with calcification 1~2 mm or >2 mm (P<0.05), and there was no significant difference between Group 1~2 mm and Group >2 mm (P>0.05). Among macro-calcified (>1 mm) thyroid nodules, calcification located in the center of the nodule was associated with malignancy while peripheral calcification was associated with benign nodule (P=0.003). However the ultrasonic features, including the quantity and acoustic shadow of the calcification had no significant effect on the diagnosis of thyroid cancer (P>0.05). Conclusion: Calcification ≤1 mm or macro-calcification located in the center of the nodule could be significant indications of thyroid cancer. 2019 Vol. 49 (1): 34-37 [Abstract] ( 951 ) HTML (1 KB)  PDF (1305 KB)  ( 1041 ) 38 Preliminary study on the protective effect of wogonin on endotoxin-induced acute lung injury mice and its mechanism GE Jinlin, YU Wenwen, ZENG Yufeng, JINChenci, DAI Yuanrong DOI: 10.3969/j.issn.2095-9400.2019.01.009 Objective: To explore the therapeutic effect of wogonin on mice with LPS-induced acute lung injury and its possible mechanism. Methods: The acute lung injury model was constructed by LPS (1 μg/kg) endotracheal instillation using C57/BL6 mice. The experiment was composed of 3 groups: control group, LPS-induced acute lung injury group (LPS group), and wogonin treatment group (WOG group). Flow cytometry and ELISA were used to detect the content of immune cells (neutrophils and macrophages) and the secretion of inflammatory cytokines in bronchoalveolar lavage fluid (BALF). Lung tissues of mice in each experimental group were also collected for hematoxylin-eosin (HE) staining. Finally, the molecular mechanism of the protective effect of wogonin on acute lung injury mice was investigated by analyzing the common downstream pathway of LPS, namely NF-κB signaling pathway. Results: Wogonin can significantly reduce the wet-to-dry (W/D) ratio of lung tissue (P<0.05), the infiltration of inflammatory cells in lung tissue, and the production of TNF-α, IL-1β, IL-6 and IL-10 inflammatory cytokine (P<0.01). The results of Western blot showed that wogonin can significantly inhibit the NF-κB signaling pathway. Conclusion: Wogonin possesses potential protection against LPS-induced acute lung injury through its anti-inflammatory property, and its potential mechanism is associated with NF-κB signaling pathways. 2019 Vol. 49 (1): 38-42 [Abstract] ( 909 ) HTML (1 KB)  PDF (1821 KB)  ( 941 ) 43 Synthesis of zinc hyaluronate and application in wound repair in mice ZHANG Haijuan, ZHANG Qilu, XU Ying, XU Lifei, JIN Liujun, XU Ke DOI: 10.3969/j.issn.2095-9400.2019.01.010 Objective: To react sodium hyaluronate with zinc chloride, then identify the physicochemical properties of the zinc hyaluronate, and observed its effect on wound healing in mice. Methods: The rubber ring was sewn with eight stitches onto the skin of 10 mice, half male and half female, to make a full-thickness wound model of the skin on the left and right sides of the mice. The left and right side were respectively coated with zinc hyaluronate solution and sodium hyaluronate solution, wrapped in sterile gauze. The medicine was changed once a day, and the wound healing of each group was observed after the operation. The sodium ion detector was used to detect the formation of zinc hyaluronate, and then the antibacterial effect of zinc hyaluronate was studied using spread plate technique. Elemental analysis was made to detect the sodium and zinc ions content of zinc hyaluronate. Results: Sodium hyaluronate reacted with zinc chloride to form zinc hyaluronate, in which zinc ions were covalently bonded with hyaluronic acid. Zinc hyaluronic acid solution also had antibacterial function (P<0.05). The results of whole skin resection in mice showed that zinc hyaluronate was more effective than sodium hyaluronate to promote wound repair (P<0.05). Conclusion: Hyaluronic acid and zinc can be combined by covalent bond, and zinc hyaluronate solid with high purity can be obtained by dialysis. Zinc hyaluronate can promote wound healing better than sodium hyaluronate. 2019 Vol. 49 (1): 43-46，51 [Abstract] ( 1115 ) HTML (1 KB)  PDF (1612 KB)  ( 1344 ) 47 The value of sonohysterography combined with quantitative analysis of three-dimensional power Doppler imaging in the diagnosis of intrauterine lesions HUANG Hu, YU Beibei, ZHAO Yaping, YANG Yan, JIN Huipei, ZHOU Xiuping DOI: 10.3969/j.issn.2095-9400.2019.01.011 Objective: To investigate the value of saline infusion sonohysterography (SIS) combined with quantitative analysis of three-dimensional power Doppler imaging (3D-PDI) in the differential diagnosis of intrauterine lesions. Methods: SIS and 3D-PDI were carried on 109 cases of married women with transvaginal ultrasonography (TVS) prompting intrauterine lesions. A quantitative analysis was made by VOCAL software and the pathological results were compared. Results: Of all the 109 patients, there were 40 cases of endometrial polyp, 48 cases of submucosal fibroid, 16 cases of intimal hyperplasia and 9 cases of blood clot. The accuracy rate, specificity and sensitivity of TVS combined with color Doppler flow imaging (CDFI) in diagnosis of endometrial polyp were 78%, 80%, 75% respectively, while those of SIS combined with 3D-PDI were 98%, 97%, 98% respectively. The differences in VI, FI and VFI between endometrial polyp group and submucosal fibroid group were significant (P<0.05). Conclusion: SIS can show clearly the intrauterine lesions while 3D-PDI can display the lesion types of perfusion and quantitative analysis is able to be performed. The combination of both procedures may significantly improve the sensitivity and specificity of intrauterine lesion diagnosis. 2019 Vol. 49 (1): 47-51 [Abstract] ( 708 ) HTML (1 KB)  PDF (1416 KB)  ( 877 ) 52 The implement of micro-scissor for hysteroscopic adhesiolysis in reproductive medicine center FU Xiaohua, JI Mengxia, SHU Jing DOI: 10.3969/j.issn.2095-9400.2019.01.012 Objective: To compare the effectiveness of micro-scissor with electric-loop in hysteroscopic adhesiolysis for moderate- to severe-intrauterine adhesion women with fertility intention. Methods: In this study, 82 women from Zhejiang Provincial People’s Hospital during September 2014 to December 2016 underwent hysteroscopic division procedure were allocated to micro-scissor group (n=39) and electric-loop group (n=43). Oral estrogen was administered to promote endometrial regeneration at operation period mense day 3, and an IUD was inserted after operation to prevent adhesion reformation. A second look hysteroscopic was performed in all cases, and the effect of hysteroscopic adhesiolysis was scored by the American Fertility Society Classification system (AFS). Pregnant outcome, including natural and assisted reproductive pregnancy, was also followed up for one year after operation. Results: At the second look, there was no significant difference in the post-operative recovery of uterine shape between two groups. However, the recovery of menstruation was significantly better in the micro-scissor group than the electric loop group. In addition, the pregnancy rate in the micro-scissor group was 43.6%, which was also higher than 37.2% in the electric loop group. Conclusion: Using micro-scissor or electric-loop in hysteroscopic adhesiolysis could improve uterine morphology and menstrual pattern to a certain extent. Treatment with micro-scissor may be a good try in women with fertility intention. 2019 Vol. 49 (1): 52-55 [Abstract] ( 791 ) HTML (1 KB)  PDF (1285 KB)  ( 1053 ) 56 Influencing factors and the reference frame for the ratio of residual volume to total lung capacity in the elderly PAN Jing, ZHENG Yongke, DAI Xinjian, YE Shaoming DOI: 10.3969/j.issn.2095-9400.2019.01.013 Objective: To explore the influencing factors and the rationality of the current criteria for judging the ratio of residual volume (RV) to total lung capacity (TLC) in the elderly. Methods: A total of 587 healthy elderly patients were enrolled in this research from January 2016 to January 2018 in Wenzhou Central Hospital. According to the standard, 190 cases including 105 males and 85 females were selected and tested by MasterScreen pulmonary function analyzer. The relevant parameters were recorded for statistical analysis. Results: Age, sex, height and body mass had a significant effect on RV/TLC (P<0.05); age increase had no effect on RV (P>0.05), but had significant effect on TLC (P<0.01). Pearson correlation analysis showed RV/TLC was positively correlated with age (r=0.297, P<0.001), negatively correlated with height (r=-0.329, P<0.001) and body mass (r=-0.246, P=0.001). Multiple stepwise linear regression showed that 3 variables, age, sex and height, entered the regression equation: RV/TLC=41.704+0.253×age+2.240×sex (males=1, females=2) -0.152×height (cm) (r=0.462, F=16.249, P<0.001). There were three different criteria for RV/TLC>35%, Shanghai’s old prediction formulas and new prediction formulas (the ratio of measured value to predicted value is>1.2), the positive rates were 68.95% (131/190), 5.26% (10/190) and 8.42%/(16/190) respectively, and there was statistical difference between each other (P<0.05). Conclusion: Age, sex, height and body mass all had effects on RV/TLC. It is probably unreasonable that RV/TLC>35% is used as a critical point for the elderly and it is suggested that a new prediction formula for the elderly should be established. 2019 Vol. 49 (1): 56-58，62 [Abstract] ( 832 ) HTML (1 KB)  PDF (1325 KB)  ( 891 ) 59 The relationship between high-risk human papillomavirus infection and vaginal microecology ZHANG Weiling, ZHAO Shanqiong DOI: 10.3969/j.issn.2095-9400.2019.01.014 Objective: To explore the relationship between HR-HPV infection and the microecological changes of vagina. Methods: From November 2016 to October 2017, sixty cases of HR-HPV infection positive in the Gynecology Department of Ningbo Hospital of TCM were selected as infection group, and sixty cases with HR-HPV infection negative were collected as control group. Two groups were examined for vaginal microecology, and the relationship between the results of vaginal microecological examination and HR-HPV infection was analyzed. The nine indicators including vaginal cleanliness, vaginal pH, hydrogen peroxide concentration, leukocyte esterase, bacterial vaginosis, aerobic vaginitis, trichomonal vaginitis, vulvovaginal candidiasis and mixed infection were analyzed by single factor. Taking the HR-HPV infection as the dependent variable to conduct logistic regression analysis. Results: There were significant differences in vaginal cleanliness, vaginal pH, hydrogen peroxide concentration, leukocyte esterase and BV between the two groups (P<0.05). It was showed that vaginal cleanliness, vaginal pH and BV were the risk factors of the HR-HPV infection (OR=8.389, 3.736, 4.222). Conclusion: The imbalance of vagina microecology is closely related to HR-HPV infection. Vaginal cleanliness degree III-IV, vaginal pH greater than 4.5 and BV are the risk factors of HR-HPV infection. 2019 Vol. 49 (1): 59-62 [Abstract] ( 935 ) HTML (1 KB)  PDF (1280 KB)  ( 969 )

Copyright © Editorial Board of JOURNAL OF WEZHOU MEDICAL UNIVERSITY Supported by:Beijing Magtech