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2018 Vol. 48, No. 12 Published: 2018-12-12   859 he clinical and genetic analysis of Leber hereditary optic neuropathy with mitochondrial ND1 gene 3497C>T mutation LIANG Min, HOU Lingling, JIANG Feng, GUAN Minxin Objective: To investigate the clinical and molecular characteristics of six Leber hereditary optic neuropathy (LHON) families with mitochondrial ND1 gene 3497C>T mutation. Methods: Clinical data were collected from six pedigrees, and detailed ophthalmology examination and mitochondrial genomes were conducted for the six probands. Then we measured the activities of respiratory complexes using the cybrid cell models from six probands and two controls. Results: Only one person in each of the six families exhibited the variable severity and age-at-onset in visual dysfunction. The affected matrilineal relatives consisted of 5 males and 1 female. All patients had pale disc and unclear boundary. Optical coherence tomography (OCT) revealed that the thickness of retinal nerve fibers (RNFL) in two patients were significantly thinner in nasal and temporal quadrant, while the other patients had no differences in all quadrants. The activity of complex I in the mutant cell lines carrying 3497C>T mutation were 87.6% relative to control cell lines. However, the activities of complex II, III and IV in mutant cell lines were comparable to those of two control cell lines. Conclusion: LHOH patients exeist different levels of fundus lesions. Mitochondrial 3497C>T mutation plays an important role in the occurrence of LHON, and this site can be added to the list of inherited factors for LHON screening. 2018 Vol. 48 (12): 859-863 [Abstract] ( 723 ) HTML (1 KB)  PDF (1833 KB)  ( 782 ) 866 Effects of NMDA receptor NR2A and NR2B subunits on pentetrazol induced chronic epilepsy rats XU Huiqin, DU Yanru, SHEN Jingzan, LIN Jiahe, DING Siqi, ZHENG Rongyuan, WANG Xinshi, WANG Li, YE Mengqian Objective: To explore the role and mechanism of N-methyl-D-aspartic acid (NMDA) receptor NR2A and NR2B subunits in pentetrazol (PTZ) induced chronic epileptic rats. Methods: Forty-eight male SD rats were randomly divided into control group, PTZ group, PTZ+MK-801 group, PTZ+NVP group, and PTZ+Ifenprodil group. Rats were daily kindled by intraperitoneal injection of 35 mg/(kg·d) PTZ for 28 days. Half an hour before PTZ kindle, rats were given intraperitoneal injection of 3.5 mL/(kg·d) saline, 0.1 mg/(kg·d) MK-801, 5.0 mg/(kg·d) NVP, and 10 mg/(kg·d) Ifenprodil, respectively. Seizure scores according to Racine standard were recorded for 28 consecutive days. All rats were sacrificed on the 29th day. Nissl’s staining was used to evaluate neuron loss of hippocampal CA1, CA3, and DG areas. Western blot was used to detect the expression of NR2A, NR2B subunits. Results: Compared with the PTZ group, MK-801 and NVP could reduce the intensity of convulsion attack in pentetrazol induced chronic epileptic rats, while Ifenprodil produced no such effect. Compared with the control group, hippocampal neurons in the PTZ group lost significantly; while compared with the PTZ group, hippocampal neuron losing in MK-801, NVP, and Ifenprodil groups were light, of which, MK-801 and NVP were the lightest. Compared with the control group, the levels of both NR2A and NR2B subunits in hippocampal decreased after convulsion attack and the decline was most pronounced in the PTZ group. Level of NR2A subunit in PTZ+NVP group was higher than PTZ group, while levels of NR2B subunit in PTZ+MK-801, PTZ+NVP and PTZ+Ifenprodil groups were higher than PTZ group. Conclusion: Non-selective inhibition of  NMDA receptor and selective inhibition of NR2A subunit could reduce the intensity of convulsion attack and improve hippocampal neuron losing in pentetrazol induced chronic epileptic rats, while selective inhibition of NR2B subunit could only improve hippocampal neuron losing. This study suggested that the convulsion attack could be related to NR2A subunit, and the damage of neurons could be related to both NR2A and NR2B subunit. 2018 Vol. 48 (12): 866-870 [Abstract] ( 734 ) HTML (1 KB)  PDF (1737 KB)  ( 1118 ) 871 Effects of acute and chronic hypoxia-hypercapnia on mitochondrial biogenesis in rat hippocampus and its relationship with cognitive impairment MIN Jingjing, GU Qun, CHEN Qi, WANG Xiaotong. Objective: To investigate the relationship between changes of mitochondrial biogenesis and learning and memory impairment in rats after acute or chronic exposure to hypoxia-hypercapnia. Methods: Forty eight SD male rats were randomly divided into three groups by the random number tables method as follows: the normal control group, the acute hypoxia-hypercapnia group, the chronic hypoxia-hypercapnia group. The cognitive ability was assessed by the Morris water maze. The mitochondrial structure was observed by the electron-microscopy. The protein expression of PGC-1α, NRF-1, TFAM were measured by the western blot. The copy number of mtDNA was detected by Real-Time PCR. Results: After exposure, the acute group performed almost similar to the normal control group in exploring the hidden platform. While the chronic group displayed worse performance during navigation test (P<0.05). In the probe trial on the last day, the acute group showed no difference with the normal control group, the crossing number of the chronic group was decreased (P<0.05). The electron-microscopy showed the mitochondrial structure of the acute group showed no obvious damage. The mitochondrial membrane of the chronic group was edema and obscure, partially destroyed, and the cristae was loosen and disorganized, partially disappeared. Western blot showed the protein expression of PGC-1α (P<0.01), NRF-1 (P<0.05), TFAM (P<0.05) were significantly increased in the acute group, but all markedly decreased in the chronic group. Real time PCR showed acute exposure induced an increase of the mtDNA copy number (P<0.01), while the expression in the chronic group significantly down-regulated (P<0.05). Conclusion: The downregulation of mitochondrial biogenesis may contribute to the cognitive function defects of long-term hypoxia-hypercapnia exposure rats. 2018 Vol. 48 (12): 871-875，,880 [Abstract] ( 621 ) HTML (1 KB)  PDF (1661 KB)  ( 766 ) 876 The effects of Astragalus polysaccharides on nitric oxide in human umbilical vein endothelial cells induced with palmitic acid HU Lanlan, CHEN Jiajun, CHEN Jundixia, PAN Xiaoqiong, HU Zhen. Objective: To explore and investigate the effects of Astragalus polysaccharides on nitric oxide in human umbilical vein endothelial cells induced with palmitic acid. Methods: Endothelial cell injury model was established by using human umbilical vein endothelial cells induced with palmitic acid. The experiment consisted of control group, model group, astragalus polysaccharide group (400 mg/L, 800 mg/L, 1 600 mg/L). The effects of Astragalus polysaccharides on cell viability was tested by MTT assay. Western blotting assay was used to detect the expressions of AMPK, p-AMPK, p-Akt, eNOS, p-eNOS, Nox4, and GAPDH. The Q-PCR assay was used to detect the expressions of Nox4 mRNA and eNOS mRNA. Nitrate reduction assay was used to detect intracellulr NO levels. Results: Astragalus polysaccharide could up-regulate the expressions of p-AMPK, p-Akt, p-eNOS and eNOS mRNA in human umbilical vein endothelial cells induced with palmitic acid, down-regulate the expressions of Nox4 and Nox4 mRNA, and increase the production of NO in cells. Conclusion: Astragalus polysaccharides can promote the production of NO in endothelial cells, which can prevent and treat the damage of vascular endothelial cells. Its regulation mechanism may be associated with its ability to activate intracellular AMPK, Akt, active eNOS, and decrease the expression of Nox4. 2018 Vol. 48 (12): 876-880 [Abstract] ( 576 ) HTML (1 KB)  PDF (1747 KB)  ( 764 ) 881 The role of type I histone deacetylase inhibitor in cartilage degradation GU Xiaopeng, CHAO Haichao, GU Yuequan. Objective: To investigate the protective effect and possible mechanism of I type HDAC inhibitor in cartilage degradation. Methods: An unstable dementabilization of the medial meniscus (DMM) model was used to assess the inhibition of HDAC by trichostatin A (TSA) in vivo. Cytokines, TSA, MS-275, valproic acid and siRNA were applied to human articular chondrocytes (HACs) and sw-1353 hondrosarcocma cells. And RT-PCR was used to confirm how they influence the expression of metalloproteinase. The activity of HDAC inhibitor was tested by western blot. The absorption of cartilage in vitro was examined by a pig’s nasal cartilage explant test. Results: Systemic administration of TSA in the developmental process (DMM) can protect cartilage. TSA, valproic acid, and MS-275 in human articular chondrocytes (HACs) inhibited the expression of MMP1 and MMP13 which was induced by cytokine, and after knocking out each type I HDAC, MMP13 expression which was induced by interleukin-1 decreased. Conclusion: MS-275 inhibition to type I HDAC (HDAC-1, HDAC-2, HDAC-3) and HDACs specificity consumption can inhibit the expression of metalloproteinase induced by cytokines in chondrocytes and PNC explants, thereby inhibiting cartilage absorption. These observations indicate that specific type I HDACs inhibitors can block cartilage degradation to some extent. 2018 Vol. 48 (12): 881-887,893 [Abstract] ( 632 ) HTML (1 KB)  PDF (2218 KB)  ( 861 ) 888 Effect of YC-1 on cognitive function and hippocampal neuronal apoptosis in aged mice after partial hepatectomy GE Yeying, WANG Xiuzhen, YUAN Na, YUAN Liyong, CHEN Jie Objective: To investigate the effect of 3-(5’-hydroxymethyl-2’-furyl)-1-benzylindazole (YC-1) on cognitive function and hippocampal neuronal apoptosis in aged mice after partial hepatectomy, and to discuss the role of hypoxia inducible factor-1α (HIF-1α) in the mechanism of post-operative cognitive dysfunction (POCD). Methods: Forty-eight healthy male type C56BL/6 mice, aged 18 months, weighing 25-35 g, were used in the study. The mice of either type were randomly divided into 3 groups (n=16), using a random number table: sham operation group (sham group), operation group (operation+NS group) and HIF-1α inhibitor YC-1 group (operation+YC-1 group). Operation models were established by partial hepatectomy and the YC-1 treatment was given to the operation group. In sham group, the mice were incised and given surgical suture on the same surgical site in partial hepatectomy. Then each group was divided 1 d and 3 d respectively (n=8). Hidden and cued platform Morris water maze training and testing was used to observe spatial memory abilities on 1st or 3rd day. After the Morris water maze test, mice in each group were sacrificed after anesthesia. The hippocampus was separated for detection of the expression of HIF-1α and Bax, Bcl-2, activated caspase-3 by Western blot. Results: Compared with sham group, the latency for mice to find the flat was obviously prolonged and the time taken to cross the flat were decreased in the Morris water maze test. The expression of HIF-1α, Bax, activated caspase-3 were significantly increased and Bcl-2 was decreased at 1 day and 3 days in the operation group (P<0.01). Compared with the operation+NS group, the latency to find the flat was obviously decreased and the times to cross the flat were increased; the expression of HIF-1α, Bax, activated caspase-3 were significantly decreased and Bcl-2 increased at 1 day and 3 days in the operation+YC-1 group. Conclusion: YC-1 can improve the cognitive function and ameliorate the hippocampal neuronal apoptosis after partial hepatectomy. It was induced by down regulation of HIF-1α protein. HIF-1α is partially involved in the mechanism of POCD induced hippocampal neuronal apoptosis in mice. 2018 Vol. 48 (12): 888-893 [Abstract] ( 676 ) HTML (1 KB)  PDF (1920 KB)  ( 846 ) 894 Over-expression of SIRT4 in inhibiting the proliferation of gastric cancer cells through G1 cell cycle arrest HUANG Guoyu, LIN Yao, LIN Jiahao, CHEN Xuxu, LI Junjian, ZHU Guanbao. Objective: To investigate the effect and mechanism of SIRT4 on the proliferation and cell cycle of gastric cancer cells. Methods: We constructed SIRT4-overexpressed gastric cancer cell-lines SGC-7901 and MNK45 by lentiviral-mediated transduction. On studying cell viability and proliferation activity, we noted the effects of overexpressed SIRT4 on the proliferation and clone forming ability of gastric cancer cells in vitro. In addition, we studied the cell cycle in an attempt to gain insights into the cellular mechanism. Results: Overexpression of SIRT4 significantly inhibited the proliferation and clone forming ability of the gastric cancer cells SGC-7901 and MNK45 in vitro. Furthermore, overexpression of SIRT4 induced G1 cell cycle arrest via downregulation of p-ERK, cyclin D, and cyclin E. Conclusion: SIRT4 may play a role as a tumor suppressor gene in gastric cancer. 2018 Vol. 48 (12): 894-897 [Abstract] ( 605 ) HTML (1 KB)  PDF (1645 KB)  ( 698 ) 898 An evaluation of chemotherapy in the treatment of advanced gastric cancer by dual-energy CT CHEN Lifang, FU Gangze, HUANG Dingpin, MAN Yi, JIN Yin, DONG Qiantong, HUANG Yingbao, CHEN Yongchun, WANG Hongqing Objective: To explore parameters for the objective evaluation and prediction of chemotherapy efficiency by characterizing the imaging parameters of dual-energy CT scanning before and after chemotherapy in patients with advanced gastric cancer. Methods: Fifty-two patients with advanced gastric cancer confirmed by gastroscopy and pathology were collected consecutively. Dual-energy enhanced CT scanning was performed before and after chemotherapy to obtain the morphological and functional parameters of the tumors, including maximum diameter, maximum thickness, maximum cross-sectional area, volume, standardized iodine value in both artery and venous phases and volume iodine content. The changes of each parameter were also calculated. According to RECIST1.1 criteria, patients were divided into chemotherapy effective group and chemotherapy ineffective group. Mann-whitney U was used to compare parameters. Results: After chemotherapy, all values of the parameters in the chemotherapy effective group were significantly decreased (P<0.05), with the change of volume iodine content in artery and venous phase being the most significant. Changes in maximum thickness, maximum cross-sectional area, volume and iodine content in artery and venous phases in chemotherapy effective group were significantly higher than those in the ineffective group (P<0.01). Before chemotherapy, the maximum thickness in the effective group was significantly higher than that in the ineffective group (P=0.036). Conclusion: The changes of volume iodine content in venous phase by dual-energy CT might be a new parameter for the evaluation of chemotherapy efficiency, and together with morphological parameters, it may provide an accurate prediction for the chemotherapy effect on the advanced gastric cancer. 2018 Vol. 48 (12): 898-902 [Abstract] ( 675 ) HTML (1 KB)  PDF (1567 KB)  ( 832 ) 903 Three-dimensional cephalometric analysis by CBCT on the craniofacial characteristics and airway volume of snoring in adolescents QIN Huaxiang, WANG Haiyan, WANG Xiaofei, CAO Zhensheng, HU Rongdang Objective: To estimate the craniofacial morphology and airway volume of young snoring patients by CBCT 3D cephalometry. Methods: A cluster sampling method was used to investigate the snoring status of 3 300 young students in Wenzhou Medical University. According to the degree of snoring, 102 subjects with severe snoring were selected to do Epworth sleepiness score, and 28 subjects scored higher than 9 did polysomnography examination (PSG). According to the PSG results, 20 subjects with snoring but not OSAHS were generalized as snoring group, and another 20 subjects, who were confirmed by himself and four of his roommates with no snoring, were defined as control group. CBCT was taken under the same conditions in both groups. Three orthodontists used Dolphin software to perform three-dimensional cephalometric and airway volume measurements on CBCT images. Each person was measured twice, one week apart. Intra-group correlation coefficient (ICC) was calculated to evaluate the reliability between six measurements, and t test was used to analyze the difference between snoring group and control group. Results: The BMI (P=0.028) and neck circumference (P=0.043) in the snoring group were larger than those of the control group. The N-S-Ar angle (P=0.042) and Nasal floor to FH angle (P<0.001) in the snoring group were larger than those in the control group, and the S-N (P=0.039), S-Ba (P=0.009) and N-ANS (P=0.022) in the snoring group were smaller than those in the control group. UP-PhW (P=0.001), UT-PhW (P=0.037), PP-SPU (P=0.006), PNS-SPU (P= 0.004), SAM-SPM (P=0.009), SAL-SPL (P=0.009), PNR (P=0.011) representing the airway space in the snoring group were significantly smaller than the control group. The airway volume of the snoring group was significantly smaller than the control group. Conclusion: The main craniofacial characteristics of young snoring people include small nasal cavity, narrow pharyngeal cavity, narrow airway, obesity and larger neck circumference. 2018 Vol. 48 (12): 903-908 [Abstract] ( 778 ) HTML (1 KB)  PDF (1594 KB)  ( 789 ) 909 Correlation of Fsp27 with hepatic fibrosis pathology and liver fibrosis index YU Xia, HUANG Kate, YU Fuxiang. Objective: To study the correlation of Fsp27 with liver fibrosis pathology and liver fibrosis index. Methods: In 45 cases of liver surgery from July 2015 to July 2017 in the First Affiliated Hospital of Wenzhou Medical University, HE staining, Fsp27 protein staining and pathological staging were performed. The expression of Fsp27 in liver was determined by immunohistochemical assay, Real-time PCR and Western blot respectively. Results: The positive expression of Fsp27 in liver tissue was mainly in primary hepatic stellate cells. Compared with normal control group, the expression of Fsp27 at mRNA and protein level was negatively correlated with the stage of hepatic fibrosis (P<0.05). There was also a negative correlation between the levels of Fsp27 and collagen III, hyaluronic acid and hydroxyproline expression (r=-0.521, -0.834, -0.667, P<0.05). Conclusion: The expression of Fsp27 is negatively correlated with the degree of hepatic fibrosis. 2018 Vol. 48 (12): 909-912 [Abstract] ( 673 ) HTML (1 KB)  PDF (1745 KB)  ( 712 ) 913 The application of Aptima HPV genotyping test in the triage of women with ASC-US WANG Kaiyi, CHEN Yingying, DONG Jie. Objective: To investigate the clinical value of Aptima high risk human papillomavirus (hr-hpv) E6/E7 mRNA (Aptima HPV) and its genotyping (GT) test in women with atypical squamous cells of undetermined significance (ASC-US) cytology for cervical cancer screening. Methods: Aptima HPV and its genotyping (Aptima HPV-GT) of the cervical exfoliated cells were detected in 770 ASC-US women. HC2-HPV test, colposcopy and cervical biopsy were performed at the same time. Results: ①In 770 women, 83.1% of high-grade cervical lesions and cervical cancer (HSIL+49/59) were detected Aptima HPV positive; ②The OR value of Aptima HPV 16 positive female exposure risk was the highest, which was significantly higher than the risk of HSIL+ in Aptima HPV 18/45+, GT- and Aptima HPV- (P<0.05). Compared with HPV GT-, the incidence of HSIL+ in GT+ women increased significantly (P<0.05); ③The specificity of Aptima HPV for detection of HSIL+ was higher than that of HC2-HPV (P<0.05), but no significant difference was found in sensitivity, positive predictive value (PPV) and negative predictive value (NPV). Conclusion: In ASC-US women, Aptima HPV and its GT detection is a reliable and effective method for cervical lesion screening and risk delamination, especially for Aptima HPV 16 positive. 2018 Vol. 48 (12): 913-916 [Abstract] ( 1077 ) HTML (1 KB)  PDF (1446 KB)  ( 1071 ) 917 The mechanism of Butein in promoting apoptosis and inhibiting metastasis of osteosarcoma cells LIN Zeng, PAN Tianlong, WU Dengying, KANG Xiaodiao, CAI Ningyu, PAN Jun. Objective: To study the effects of Butein on apoptosis and invasion and metastasis of U2OS osteosarcoma cells. Methods: U2OS osteosarcoma cells were treated with different doses of Butein for 24 h respectively. CCK-8 assay was used to detect the activity of U2OS cells. Western blot assay was used to detect the expression of  Bcl-2, Bax, MMP-2, MMP-9, and the PI3K-Akt signaling pathway related proteins. The TUNEL assay was used to detect the apoptosis of U2OS osteosarcoma cells. The above experiments were repeated three times to ensure the accuracy of the experimental results. Results: After U2OS osteosarcoma cells were treated with Butein, the cell activity of U2OS osteosarcoma was decreased significantly. The expression of Bax proteinwas increased, while the expression of Bcl-2, MMP-2 and MMP-9 was decreased. In addition, when U2OS osteosarcoma cells were treated with Butein, the expression of PI3K-Akt signaling pathway was decreased. Conclusion: Butein promotes the apoptosis of U2OS osteosarcoma and suppresses its invasion and metastasis. The results may suggest that Butein exhibits antitumor activities by suppressing the PI3K-Akt signaling pathway. 2018 Vol. 48 (12): 917-920 [Abstract] ( 654 ) HTML (1 KB)  PDF (1730 KB)  ( 658 ) 921 Prophylactic embolization in the treatment of congenital bronchial artery-pulmonary circulation fistulas HUANG Jing, ZHENG Yunyun, LI Ping, RONG Xing, ZHANG Songyue, XIA Tianhe, QIU Huixian, WU Rongzhou.  Objective: To evaluate the curative effect of the prophylactic embolization in the treatment of congenital bronchial artery-pulmonary circulation fistulas (CBPF). Methods: A total of 11 children (6 males and 5 females), who had CBPF from the Second Affiliated Hospital of Wenzhou Medical University from Jan. 2016 to Dec. 2017, were diagnosed with selective bronchial artery angiography in the procedure of interventional occlusion of patent ductus arteriosus (PDA). The age of all patients ranged from 11 months to 12 years old. In 10 cases, whose fistulas were larger in diameter than adjacent bronchial artery, interventional occlusion was performed by either interlock fibered coils or pfm coils. Regular follow-up was done in 1, 3, 6 and 12 months after operation and once a year thereafter. Results: All patients’ PDA were successfully occluded. After PDA occlusion, the descending aortic angiography was applied, which showed that all 10 patients’ PDA were completely closed immediately, but 1 patient was left untreated due to the tortuosity of bronchial artery. During the follow-up, no patient had hemoptysis or other complications. Conclusion: Prophylactic therapeutic embolization is an effective way to protect patients who have CBPF. However whether it can protect against major hemoptysis remains unknown and requires long-term follow-up. 2018 Vol. 48 (12): 921-924,928 [Abstract] ( 693 ) HTML (1 KB)  PDF (1597 KB)  ( 875 ) 925 The role of immature platelet fraction in the prediction of platelet transfusion in cancer patients after chemotherapy GUO Jingjing, SHEN Wencai, YE Xuanmei, PAN Suzhen, ZHOU Lianlian, JIANG Minghua. Objective: To investigate whether immature platelet fraction (IPF%) predicate imminent platelet recovery after chemotherapy for the cancer patients, so that unnecessary platelet transfusion can be avoided. Methods: A total of 186 patients were enrolled and divided into blood transfusion group and non-transfusion group. IPF% of anticoagulated blood with EDTA-K2 was evaluated by means of the IPF master program equipped with SYSMEX XE-5000. The differences between IPF% and PLT of blood transfusion group were analyzed dynamically. The ROC curve was performed to evaluate the effect of those indictors in predicting whether PLT transfusion was needed. Results: The IPF% in the blood transfusion group [5.10% (2.48%, 10.68%)] was higher than that in the non-transfusion group [1.85% (1.20%, 3.50%)], and the blood platelet count in the blood transfusion group [35.00×109/L (12.75×109/L, 123.75×109/L)] was lower than in the non-transfusion group [150.50×109/L(94.75×109/L, 209.00×109/L)] (P<0.05). There was an increase of IPF% during PLT nadir. The results of ROC analysis demonstrated that there was no statistical difference between the AUC of PLT and IPF%. Conclusion: Increased IPF% indicates PLT destruction in peripheral blood, but IPF% measurement lacks the predictive power regarding imminent PLT recovery in cancer patients. Therefore decisions regarding prophylactic PC transfusions should not be based on this parameter. 2018 Vol. 48 (12): 925-928 [Abstract] ( 628 ) HTML (1 KB)  PDF (1492 KB)  ( 756 ) 929 The current situation of kinesiophobia and its influencing factors in chronic pain patients WANG Songchao, ZHANG Chunme, WANG Rong, WANG Linyan Objective: To understand the current situation of kinesiophobia and its influencing factors in chronic pain patients, providing reference for the medical staff to take targeted intervention measures. Methods: A survey of 176 patients suffering from chronic painswas madein the out-patient departments of the three third-level grade A hospitals in Zhejiang Province. Convenient sampling methods ofthe general questionnaires, the Changhai pain rating scale and tampa scale of kinesiophobia (TSK) were used in the study. Results: The TSK score was (42.92±3.47) and the rate of movement fear was up to 42.61%. The single factor analysis showed that educational background, current physical pain and the previous maximum pain level had statistical significance (P<0.05). Logistic regression analysis showed that the previous maximum pain level was the major influencing factor for kinesiophobia (OR=1.468, 95%CI=1.225-1.760, P<0.01). Conclusion: The chronic pain patients are more likely to suffer from kinesiophobia, and there is a close connection between the previous most painful level and fear of moving. It is suggested that we should pay attention to the previous pain level of patients with chronic pain, change the patients’ cognition of movement fear and promote the rehabilitation of patients with chronic pain. 2018 Vol. 48 (12): 929-932 [Abstract] ( 690 ) HTML (1 KB)  PDF (1491 KB)  ( 1057 )

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