Home  |  About Journal  |  Editorial Board  |  Instruction  |  Subscribe  |  Messages Board  |  Contact Us  |  中文

Forthcoming Articles Current Issue Archive Advanced Search Read Articles Download Articles Email Alert

2018 Vol. 48, No. 11 Published: 2018-11-25   781 The experimental study of the relationship between cadmium exposure during pregnancy and pregnancy-related hypertensive disorders Wang Fan, Qiu Haifan, Liu Yi, Chen Lulu, Fan Fengyun, Wang Zongmin Objective: To explore the cadmium’s toxicological effect on development of pregnancy-related hypertensive disorders through cadmium exposure during pregnancy in rats. Methods: Pregnant SD rats were divided into three groups (0.25 mg/kg group, 0.5 mg/kg group and control group) given intraperitoneal injection of different dose of cadmium chilorde (0.25, 0.5 mg/kg) and saline every day from gestational day (GD) 4 to 19. Non-pregnant rats received an intraperitoneal injection of 0.5 mg/kg cadmium chilorde as non-pregnant controls. The effect of cadmium on systolic blood pressure was measured by BP-98A blood pressure meter. And the effect of cadmium on urinary albumin excretion was investigated by BCA protein assay kit. This study also investigated the morphological changes in placenta, kidneys, liver, brain by HE staining, and ultrastructure of placental trophoblast cells were observed with transmission electron microscopy in pregnant rat exposed to cadmium. Results: The systolic blood pressures of rats at 12 and 19 days of pregnancy was significantly elevated after injection of cadmium at dose of 0.5 mg/kg compared with that in control group and non-pregnant group at the same dose (P<0.05). The urinary albumin excretion of rats in 0.5 mg/kg group was significantly higher compared with that in other three groups at gestational day 19 (P<0.01). The morphological changes in placenta induced by cadmium included thickening of the media vessel walls, degeneration and excessive perivillous fibrin depositionin placental labyrinth. The swelling of the glomerular endothelia cells and thickening of the media renal vessel walls also were observed in kidney. Reduced placental trophoblast intracellular organelles and papilla were revealed by electron microscope. Conclusion: The key features of pregnancy-related hypertension can be observed in rat after injection with a relatively low concentration of cadmium. Through further evaluation of the reproductive toxicological characteristics of cadmium during pregnancy, it is found that cadmium induces pathological damage in the placenta, kidney, liver, and brain of rats, which is similar to pregnancy-related hypertension in human in pathophysiological aspects. 2018 Vol. 48 (11): 781-785 [Abstract] ( 901 ) HTML (1 KB)  PDF (1757 KB)  ( 937 ) 786 Effect of amiodarone on oxidative stress of fibroblasts and the intervention effect of panax notoginseng saponin R1 TU Mengyun, WANG Zhiyi, WAN Xinlong, WENG Jie, XIE Mengying, ZHENG Xiaoqun Objective: To study the effects of different concentrations of Notoginsenoside R1 on amiodarone-induced damage to human embryonic lung fibroblasts (HELFs). Methods: The HELFs were exposed to amiodarone in vitro. The experiment was divided into control group, amiodarone group, Notoginsenoside R1 intervention group and Notoginsenoside R1 control group. The morphological changes of cells were observed by inverted phase contrast microscope, CCK8 kit was used to observe cell proliferation activity, the level of reactive oxygen species (ROS) was measured by fluorescence microscope and fluorescence cytometry, enzyme bioassay was used to detect superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Results: When amiodarone was exposed to cells, the morphology of the cells was changed. After the intervention of Notoginsenoside R1, the cells showed no obvious morphological changes. Compared with the control group, amiodarone caused increased cell proliferation activity, increased intracellular ROS activity, decreased SOD activity and increased MDA content (P<0.05). Compared with amiodarone group, a certain concentration of Notoginsenoside R1 inhibited cell proliferation, reduced ROS activity and MDA levels and increased SOD activity (P<0.05). Conclusion: Amiodarone can induce cell proliferation and oxidative stress in HELFs, and Notoginsenoside R1 has a protective effect on it. 2018 Vol. 48 (11): 786-790 [Abstract] ( 649 ) HTML (1 KB)  PDF (1452 KB)  ( 1001 ) 791 The application of a fast and noninvasive method for testing the mutations associated with aminoglycoside-induced hearing loss in children ZHANG Qiongmin, LI Sisi, CHEN Jun, ZHU Yi Objective: To probe the application of a fast and noninvasive method for testing the mutations A1555G and C1494T associated with aminoglycoside-induced hearing loss in children. Methods: A total of 126 non-syndromic sensorineural deafness patients aged 2 to 15 were selected for collecting oral swabs and venous blood from the First Affiliated Hospital of Wenzhou Medical University during May 2015 to May 2017. Multiplex allele-specific PCR was used to test the mutations of A1555G and C1494T in DNA from oral swabs. Simultaneously, Sanger sequencing was used to detect the mutations of A1555G and C1494T in the DNA of vein blood to verify the reliability of the former by Kappa testing. Results: The detection rate of A1555G and C1494T mutations in DNA from oral swabs detected by multiplex allele-specific PCR was 6.35% (8/126), including 7 cases of A1555G mutations and 1 cases of C1494T mutation. The testing rate of Sanger sequencing was also 6.35% (8/126), and the two methods were in a good consistency checking (kappa=1, P<0.01). Conclusion: The testing of A1555G and C1494T mutations with multiplex allele-specific PCR in DNA from oral buccal swabs is a simple, rapid and non-invasive method to provide another option for the testing of aminoglycoside-induced deafness in infants and newborns. 2018 Vol. 48 (11): 791-795 [Abstract] ( 688 ) HTML (1 KB)  PDF (1389 KB)  ( 735 ) 796 Analysis of Nrf2 expression and oxidative stress in the placental, uterine and cardiac tissues of rats with gestational diabetes mellitus SUN Congcong, ZHENG Jiayong, WANG Wenhuan, HU Yanjun, ZHENG Jianqiong, ZHANG Hongping Objective: To analyze the varied expressions of Nrf2 and the level of oxidative stress in the placental, uterine and cardiac tissues from gestational diabetes mellitus (GDM) rats and gestational rats. Methods: Twenty pregnant SD rats were randomly divided into GDM group and normal group. GDM group rats were injected with streptozotocin intraperitoneally to induce GDM models, while rats in the control group were injected with the same amount of citric acid buffer intraperitoneally. All the rats were sacrificed at day 30 of pregnancy by anesthesia, and the placentae, uteruses and hearts were excised for protein extraction. Subsequently, the levels of insulin, SOD as well as GSH in different tissues were assayed by ELISA. The level of MDA was examined by the thiobarbital acid colorimetry. The expression level of Nrf2 was analyzed by Western blot. Results: The insulin and MDA from the placental, uterine tissues were higher in GDM group compared with the normal group (P<0.05), while GSH and SOD were lower (P<0.05). Also, the GDM rats exhibited higher Nrf2 protein level in the placental and uterine tissues compared with normal group (P<0.05). However, there was no significant difference in cardiac tissue between the two groups (P>0.05). Conclusion: GDM leads to insulin resistance, oxidative stress and the elevated Nrf2 expression in the placenta and uterine, which are unaffected in the hearts. Thus, it is speculated that GDM-induced oxidative stress and Nrf2 alteration differ among tissues. 2018 Vol. 48 (11): 796-800 [Abstract] ( 671 ) HTML (1 KB)  PDF (1628 KB)  ( 760 ) 801 The expression analysis of transmitter related vesicle transporters of hippocampal in CCI induced-vascular dementia rats CAO Yanjing, WU Jiali, LI Peng, LIN Ping, FENG Li, WANG Qin, REN Qian, XIE Xiaofeng. Objective: To investigate the expression of different vesicles transporter (different synapses) vGluTs, vAChT and vGAT in the hippocampal CA1-3 region in rats with chronic global cerebral ischemia. Methods: VaD animal model was established by BCCAO method, Western blot and immunofluorescence were used to detect the expression of vGluTs, vAChT and vGAT in hippocampal CA1-3. Results: In the Morris test, the escape latency of VaD rats was significantly extended compared with sham and control animals (P<0.05). The percentage of searching time in the VaD group was significantly reduced (P<0.05) and the searching distance in target quadrant was significantly decreased (P<0.01). In the T-maze test: the error rate of the delayed operation of VaD rats was significantly increased (P<0.01). The levels of vGluT1 and vGluT3 in the hippocampal CA1-3 region of VaD rats were significantly lower than those in the sham and control groups (P<0.01). Compared with the sham and control groups, the expression of vAChT in the hippocampal CA1-3 region of VaD rats was decreased (P<0.01). Compared with sham and control groups, the number of vGluT1 and vGluT3 positive neurons in VaD rats decreased in hippocampal CA1-3 region, and the number of vAChT positive neurons in hippocampal CA1-3 was also significantly decreased (P<0.01). Conclusion: Glutamate, cholinergic vesicle cargo function defects, glutamate, central cholinergic neurotransmitter imbalance, neuronal loss, CA1-CA3 hippocampus information loop destruction is the main cause of cognitive dysfunction in rats under the ischemic conditions. 2018 Vol. 48 (11): 801-806,812 [Abstract] ( 602 ) HTML (1 KB)  PDF (1798 KB)  ( 777 ) 807 Evaluation of left atrial minimum volume and left atrial maximum volume in assessing anthracycline-induced cardiotoxity: a repeated measurement analysis NIU Lin, ZHAO Min, ZHOU Fang, LIU Jian, BAO Lingyun Objective: To investigate the value of left atrial minimum volume and left atrial maximum volume by three-dimensional echocardiography (3DE) in evaluating anthracycline-induced cardiotoxity in breast cancer patients receiving chemotherapy. Methods: The study consisted of 53 patients who were diagnosed as breast cancer and then underwent doxorubicin chemotherapy after right breast mastectomy. Conventional echocardiographic parameters including left atrial diameters, ratio of early (E) by Doppler recordings from mitral inflow and peak early diastolic velocity (e’) of lateral mitral annulus by tissue Doppler imaging (E/e’) were performed at baseline and after every cycle of chemotherapy. Left atrial volume parameters including left atrial volume maximum index (LAVmax), left atrial minimum volume index (LAVmin), left atrial ejection volume (LASV), left atrial expansion index (LAEI) were obtained at the same time by 3DE. The above parameters were compared between baseline and after every cycles. Results: Compared with baseline, E/e’ had statistical difference after second to fourth cycle (P<0.05). There were no significant changes in E/e’ after the first chemotherapy (P>0.05). Compared with baseline, conventional echocardiography parameters had no statistically changes.  There were statistical differences in LAVmin after every cycle of chemotherapy and in LAVmax after T2, T3 and T4. There was no statistical difference in LASV, LAEI after every cycle of chemotherapy (P>0.05). There were significant correlations between LAVmin, LAVmax and E/e’(P<0.01), with correlation coefficients being 0.677 and 0.603 respectively. Conclusion: LAVmax and LAVmin are better parameters in monitoring anthracycline chemotherapy-associated cardiotoxicity and have significant correlations with diastolic function of the left ventricle. 2018 Vol. 48 (11): 807-812 [Abstract] ( 744 ) HTML (1 KB)  PDF (1370 KB)  ( 810 ) 813 The role of IGFBP-4 in breviscapine inhibited lung cancer A549 cells growth SUN Lei, LI Weiwen, LI Yuan, LAN Xiu, LYU Zhuqing Objective: To investigate the role of IGFBP-4 in breviscapine (BVP) inhibited lung cancer A549 cells growth. Methods: A549 cells were treated with BVP 0, 25, 50 and 100 μmol/L BVP for 48 h. Adv-IGFBP-4-OE™ DNA, EGFP and Adv-KD™ Easy RNAi EGFP were used to establish IGFBP-4 over- or down- expression A549 cells. MTT assay was used to detect cell survival rate, Trypan blue staining was applied to analyze cell death rate, Annexin V/Propidium iodide (PI) double staining was used to test cell apoptosis. Western blot, RT-qPCR and ELISA were used to detect the expression Bax, Bcl-2, the protein and mRNA expression and secretion of IGFBP-4. Results: The viability decreased, but apoptosis rate increased significantly in A549 cells with 25, 50 and 100 μmol/L BVP treatment. BVP also increased Bax but reduced Bcl-2 expression. The mRNA and protein expression of IGFBP-4 and the secretion of IGFBP-4 increased with BVP treatment for 48 h. The over-expression of IGFBP-4 decreased the survival rate of A549 cells, and enhanced the growth inhibition by 100 μmol/L BVP. The down-expression of IGFBP-4 increased the cell survival rate, and alleviated the growth inhibition by 100 μmol/L BVP. Conclusion: BVP has an inhibitory effect on A549 growth, which might be related to overexpression of IGFBP-4. 2018 Vol. 48 (11): 813-817 [Abstract] ( 746 ) HTML (1 KB)  PDF (1442 KB)  ( 827 ) 818 The role of E6 / E7 mRNA in the follow-up patients with high-risk human papillomavirus DNA positive and mild cytology WANG Lanying, SHI Yao, ZHANG Shengzhi, XU Ying, HE Yan, ZHU Xueqiong. Objective: To investigate the prognostic value of E6/E7 mRNA in the follow-up of patients with high-risk human papillomavirus (HPV) DNA positive and with atypical squamous cell of undetermined significance (ASCUS) or low-grade squamous intraepithelial lesions (LSIL) in cytologic test. Methods: Altogether 332 female patients with ASCUS or LSIL detected by Thin prep cytologic test (TCT) and high-risk HPV DNA positive from January 2015 to December 2015 were collected at the outpatient of Yuyao People’s Hospital. All patients underwent HPV E6/E7 mRNA detection, colposcopic biopsy and pathological examination. The patients whose pathology was CINII- were followed up for 24 months. Kaplan-Meier method was used to analyze the disease progression rate of HPV E6/E7 mRNA positive and negativegroups. Results: Of 332 patients, 277 (83.43%) were CINII- and 55 (16.57%) were CINII+. After 24-months follow-up of 257 patients with CINII-, the percentage of developing CINII+ in E6/E7 mRNA positive group (33/158, 20.89%) was significantly higher than that in negative group (7/99, 7.07%) , and the difference of cumulative disease progression rate in 24 months was statistically significant (χ2=9.966, P=0.002). HPV E6/E7 mRNA detection predicted the specificity of CINII+ to be 42.4 %, the positive predictive value was 22.4%, and the negative predictive value was 93.2%. Conclusion: HPV E6/E7 mRNA testing may be of value to select high-risk HPV DNA positive women with mild cytology in need of immediate referral for colposcopy. 2018 Vol. 48 (11): 818-821,827 [Abstract] ( 839 ) HTML (1 KB)  PDF (1348 KB)  ( 814 ) 822 Impact of sarcopenia on the postoperative function following internal fixation with femoral intertrochanteric fractures LANG Junzhe, JIN Jianfeng, WU Congcong, WU Peng, CHEN Lei. Objective: To determine the association of sarcopenia with short-term postoperative function after internal fixation for femoral intertrochanteric fractures. Methods: A total of 201 patients with intertrochanteric fractures who underwent intramedullary fixation in the First Affiliated Hospital of Wenzhou Medical University from May 2013 to January 2017 were analyzed retrospectively. The general conditions of the patients were collected before surgery, and the appendicular skeletal muscle index and grip strength were measured. The clinical prognosis was followed up, including postoperative complications, Barthel index (6 months after surgery), hospitalization expenses, hospitalization time, etc. Univariate andmultivariate logistic regression analysis was used. Draw a ROC curve and calculate the area under the curve. Results: Sarcopenia was present in 98 patients (48.8%), who had a higher risk of postoperative infection (χ2=4.430, P=0.035), cardiopulmonary complication (χ2=4.232, P=0.04), and lower Barthel Index (Z=-8.804, P<0.05) compared with non-sarcopenic patients. According to the comparison between 6 months postoperative and preoperative Barthel index, the patients were divided into the functional recovery group and the incomplete recovery group. The comparison between the groups indicated that (χ2=23.213, P<0.001), low vitamin D (t=-1.752, P=0.083), low bone density (t=2.774, P=0.006), advanced age (t=-4.678, P<0.001), lower extremity venous thrombosis (χ2=5.635, P=0.018) were possible factors related to incomplete functional recovery. Binary logistic regression analysis suggested sarcopenia (OR=2.918, 95%CI=1.300-6.553, P=0.09), advanced age (OR=1.041, 95%CI=1.009-1.074, P=0.012) and thrombosis (OR=5.177, 95%CI=1.090-24.59, P=0.039) were risk factors for incomplete recovery of postoperative function. ROC curve analysis indicated the area under the curve of sarcopenia and age were 0.320 (P<0.001) and 0.702 (P<0.001) separately. Conclusion: Patients with trochanteric fracture have a higher incidence of sarcopenia, which is an important risk factor for poor prognosis in patients with intertrochanteric fractures. 2018 Vol. 48 (11): 822-827 [Abstract] ( 684 ) HTML (1 KB)  PDF (1340 KB)  ( 709 ) 828 Bioinformatic analysis and functional prediction of liver cancer related genes CHEN Linbo, LI Xianpeng, JIANG Hao, ZENG Lili, ZHENG Jinglei, XU Feng. Objective: To explore the mechanism of liver cancer based on bioinformatics. Methods: Microarray data of Liver cancer related genes were obtained from Gene Expression Omnibus (GEO) database and DEGs were identified using R software. Functional annotations of DEGs were conducted by gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG). Then, a protein-protein interaction (PPI) network was constructed to screen hub genes. Finally, the hub genes were verified by GEPIA. Results: A total of 154 differentially expressed genes were identified in liver cancer. GO enrichment analysis revealed that DEGs were mainly involved in telomere organization, DNA replication and regulation of gene expression. KEGG pathway analysis was mainly related to mineral absorption, systemic lupus erythematosus and carbon metabolism in cancer. Ten hub genes including TOP2A, CENPF, ASPM, NEK2, CCNA2, PRC1, MELK, CCNB2, RACGAP1 and NUSAP1 were screened out by constructing PPI network. Subsequent validation in GEPIA database showed that all these 10 genes were up-regulated in liver cancer and associated with the prognosis of liver cancer patients. Conclusion: Bioinformatics can effectively screen and analyze liver cancer related DEGs, which may provide theoretical reference for further exploration of the liver cancer mechanism. 2018 Vol. 48 (11): 828-832 [Abstract] ( 919 ) HTML (1 KB)  PDF (1537 KB)  ( 1091 ) 833 Analysis of influencing factors of long-term efficacy and prognosis of central cord syndrome YE Yi, SONG Huanjun, YE Zhifang, JIANG Guoqiang. Objective: To evaluate the long-term efficacy of central cord syndrome (CCS) and analyze the influencing factors of long-term efficacy and prognosis. Methods: We retrospectively analyzed the data of 72 patients with CCS who were hospitalized at the Affiliated Hospital of Medical School of Ningbo University from January 2008 to December 2011. The neurological function of the patients was assessed by the JOA score method on admission and at the final follow-up. Univariate and multivariate analysis was performed using simple linear regression analysis and multiple linear regression analysis. Results: At the final follow-up visit, the mean JOA score increased from (10.13± 3.54) points to (14.14±2.53) points, and the JOA improvement rate was (59.49±31.80)%. The JOA improvement was significantly better than conservative treatment in the patients undergoing surgery. The difference was significant (P<0.05). After simple factor linear regression analysis and multivariate linear regression analysis of the various factors influencing the long-term efficacy and prognosis, it was shown that age and treatment were independent factors of the efficacy, and admission JOA score and hormone shock therapy were independent factors of the prognosis. Conclusion: It is less effective in the elder patients. Surgical treatment is superior to conservative treatment. Patients with severe initial neurological impairment and hormone shock treatment often have poorer prognosis. 2018 Vol. 48 (11): 833-836 [Abstract] ( 705 ) HTML (1 KB)  PDF (1308 KB)  ( 751 ) 837 Case control analysis of protracted bacterial bronchitis LI Changchang, DONG Lin, XIA Yongqiang, ZHANG Hailin, HU Xiaoguang, ZHENG Yangming. Objective: To analyze the clinical characteristics and etiology in children with protracted bacterial bronchitis (PBB) confirmed by bronchoscopy. Methods: The hospitalized children who had over 4 weeks cough from June 2014 to December 2016 were investigated retrospectively. The children were defined as PBB (n=50) who had a chronic wet cough without signs of an alternative specific cause and responding to 2 weeks of appropriate antibiotics, had positive bacterial culture and/or increased of neutrophils in bronchoalveolar lavage fluid (BALF). Those did not fulfill the above criteria were divided into the ‘no PBB group’ (n=48). Results: In the PBB group, there were 36 boys and 14 girls. The median (IQR) age was 3.2 (2.0~4.0) years and 58.0% cases were under 3 years. Thirty children were accompanied by wheezing. Airway malacia were found in five cases. The average of neutrophils in BALF was 0.63 (0.50, 0.82), which was significantly higher than that in the control group (P<0.01). 36 isolates were identified from BALF in children with PBB, the common pathogens were Streptococcus pneumoniae (n=19), Haemophilus influenzae (n=5) and Moraxella catarrhalis (n=4). All children received a 2~4 weeks course of antibiotics. The majority (n=48) received oral amoxicillin/clavulanic acid. Twenty-three cases (47.9%) had a recurrent PBB by 1 year follow-up. Conclusion: PBB occurs mainly in younger children and often co-exist with wheezing. Streptococcus pneumoniae is the leading pathogen in children with PBB. Amoxicillin/clavulanic acid is the major treatment for PBB. 2018 Vol. 48 (11): 837-841 [Abstract] ( 624 ) HTML (1 KB)  PDF (1316 KB)  ( 892 ) 842 Observation on effect and safety of memantine combined with speech training for post-stroke aphasia PAN Rongrong, HE Jincai Objective: To evaluate the efficacy and safety of speech training combined with memantine in the treatment of post-stroke aphasia (PSA). Methods: A total of 120 patients with PSA, including 60 cases in the control group, 60 cases in the observation group. Both groups were given speech training. The observation group was treated with memantine additionally. The patients in the two groups were evaluated by MMSE and CRRCAE before treatment and 1 and 3 months after treatment. The scores of the two groups were compared and the adverse reactions were observed at the same time. Results: After 3 months of treatment, the MMSE score in the observation group was significantly higher than before (P<0.05). After 1 month of treatment, the scores of listening comprehension and retelling in the observation group and the control group were significantly higher than those before treatment (P<0.05). There was no significant difference in the CRRCAE score between the observation group and the control group (P>0.05). After 3 months of treatment, the CRRCAE score in the observation group and the control group were significantly higher than those before treatment (P<0.05). The improvement of listening comprehension of nouns and sentences in the observation group was significantly better than that in the control group (P<0.05). No obvious adverse reactions were found in both groups. Conclusion: Speech training is effective in improving the language function of PSA patients, and maybe more effective when combined with memantine, especially in noun listening comprehension, sentence listening, and has a higher security. 2018 Vol. 48 (11): 842-845,849 [Abstract] ( 676 ) HTML (1 KB)  PDF (1338 KB)  ( 740 ) 846 A neuropsychological study of prospective memory impairment in postpartum women CHEN Kui, YUAN Liangjin, TANG Xiangyang, XIE Dandan, FANG Guozhen. Objective: To investigate the changes of prospective memory components (EBPM and TBPM ) in postpartum women, and explore the effect of pregnancy on prospective memory. Methods: A neuropsychological test for prospective memory and related background tests. PM, RM test and general cognitive assessment were performed on 30 healthy women with proper age, education and 30 cases of primary parturients one week after natural childbirth. Results: The score of EBPM, TBPM in the parturient group was significantly lower than that of the normal control group (6.00±0.83, 5.33±0.76 vs. 7.60±0.67, 5.93±0.25, P<0.01). There was no significant difference in RM score between two groups (5.80±1.63, 5.53±0.57 vs. 5.83±1.53, 5.63±0.56, P>0.05). There was statistical significance in the damage index of EBPM and TBPM [0.00 (-0.17, 0.00) vs. -0.25 (-0.38, -0.13), P<0.05]. Conclusion: Postpartum women suffer from impaired memory, particularly from prospective memory, and more obvious damage is found in EBPM. 2018 Vol. 48 (11): 846-849 [Abstract] ( 692 ) HTML (1 KB)  PDF (1277 KB)  ( 737 ) 850 Effect of high quality breast feeding health education program on postpartum breast syndrome and HAMA, HAMD score XU Huifang, NI Juanjuan, LI Yao, ZHANG Liping Objective: To explore the effect of high quality breast feeding health education program on postpartum breast syndrome and HAMA, HAMD score. Methods: A total of 200 pregnant women from January 2017 to August 2017 in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University were randomly divided into observation group (n=100) and control group (n=100). The patients of control group received routine nursing care while the patients of observation group received the high quality of breastfeeding health education program intervention. The postpartum breast syndrome on both group were investigated and compared. On the admission and at 12 weeks after delivery, the confidence evaluation scale, Hamilton Anxiety Scale (HAMA), Hamilton Depression Scale (HAMD) score were used to evaluate the psychological status of pregnant women, and to investigate the feeding mode of puerpera. Results: The incidence of maternal milk buldge and mastitis in the observation group were significantly lower than the control group (P<0.05). Twelve weeks postpartum, two groups of maternal confidence scale scores were significantly higher than on admission (P<0.05). Twelve weeks postpartum, confidence scale scores in the observation group were significantly higher than that in the control group (P<0.05). Twelve weeks postpartum, two group HAMA and HAMD scores were significantly lower than on admission (P<0.05). Twelve weeks postpartum, the HAMA and HAMD scores in the observation group were significantly lower than the control group (P<0.05). The maternal breastfeeding rate in the observation group was 93%, compared with 69% in the control group. The breastfeeding rate of the observation group was significantly higher than that of control group (P<0.05). Conclusion: The application of high quality breast feeding health education program can effectively reduce the incidence of postpartum breast syndrome, improve the HAMA and HAMD score, which rise the breastfeeding rate. 2018 Vol. 48 (11): 850-853 [Abstract] ( 669 ) HTML (1 KB)  PDF (1308 KB)  ( 767 )

Copyright © Editorial Board of JOURNAL OF WEZHOU MEDICAL UNIVERSITY Supported by:Beijing Magtech