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2018 Vol. 48, No. 3 Published: 2018-03-25   157 The effect of curcumin on learning-memory ability and inflammatory signaling pathway in hippocampus of APP/PS1 double transgenic mice HAN Yuan, HUANG Chenmiao, FANG Qianjuan, LIU Qixing, NAN Ke, XIANG Fangfang, CAO Hong, LI Jun Objective: To investigate the effect of feeding curcumin on learning and memory ability, amyloid-β (Aβ) and the protein expression of high mobility group box protein-1 (HMGB1), receptor for advanced glycation endproducts (RAGE), Toll-like receptors 4 (TLR4), nuclear factor kappa B (NF-κB) p65 in the hippocampus of amyloid precursor protein / presenilin 1 (APP/PS1) double transgenic mice. Methods: Male transgenic mice (n=20) were randomly divided into model group (M group, n=10) and treatment group (T group, n=10), the littermate wild-type male mice (n=10) were used as control (C group, n=10). The 4-month-old mice in T group were fed with curcumin diet (100 mg•kg-1•d-1), M group and C group were fed with normal diet. Morris water maze was applied to test the learning and memory ability for all 9-month-old mice. Aβ and the protein expression of HMGB1, RAGE, TLR4, NF-κB p65 in the hippocampus were determined by immunohistochemistry and Western blot respectively. Results: Compared with group C, the mice in group M had longer escape latencies (P<0.05) and the average number across platform was decreased (P<0.05) in Morris water maze test, the number of Aβ and the value of IOD was increased in CA1 region and DG region of hippocampus (P<0.05), while the protein expression of HMGB1, RAGE, TLR4, NF-κB p65 (P<0.05) was significantly increased. Compared with group M, the mice in group T had shorter escape latencies (P<0.05) and the average number across platform was increased (P<0.05), the number of Aβ and the value of IOD were decreased in CA1 region and DG region of hippocampus (P<0.05), the protein expression of HMGB1, RAGE, TLR4, NF-κB p65 was significantly increased (P<0.05). Conclusion: Oral curcumin for 5 months reverses the learning and memory impairment of APP/PS1 transgenic mice, and the mechanism is likely to inhibit HMGB1-RAGE/TLR4-NF-κB signaling pathway. 2018 Vol. 48 (3): 157-161 [Abstract] ( 811 ) HTML (1 KB)  PDF (1361 KB)  ( 1027 ) 162 The preparation for decellularized scaffolds in the rat uterine and its evaluation GUO Fang, YANG Jie, CHEN Jingjing, LIU Li, CHEN Yunzhi, ZHENG Feiyun Objective: To explore the feasible strategy for the preparation of natural uterine decellularized scaffolds and to evaluate it systematically. Methods: Female SD rats were used, detergents including TritonX-100 and sodium dodecyl sulfate were perfused into the uterus through the uterine artery after physical freezing and thawing and enzymatic hydrolysis. After decellularization, scaffolds were evaluated by general observation, methylene blue staining, HE staining, quantitative analysis of genomic DNA, cytokine content determination, transmission and scanning electron microscopy (TEM and SEM), collagen detection and identification. Results: After the perfusion, the transparent uterine scaffold was obtained, HE staining and TEM showed that there was no cell residual in it. DNA content was confirmed to be (45.6±7.3) ng/mg, less than 5% of the normal uterus (P<0.01). Methylene blue staining and SEM showed that vascular network and spatial structure were intact. Collagen content increased from (0.57±0.12) μg/mg in fresh uterine tissue to (0.88±0.10) μg/mg in scaffolds (P<0.05), combined with IHC study, indicating that extracellular matrix components of the scaffolds were completely preserved. In addition, ELISA results showed that the cytokines EGF, bFGF and TGF-β were still retained in the decellularized scaffolds (66%, 85% and 54%, respectively), indicating that they still had some biological activity. Conclusion: The decellularized uterine scaffold could be successfully prepared with its physical and chemical properties preserved, and it may be used as an alternative platform for uterine tissue engineering study. 2018 Vol. 48 (3): 162-167 [Abstract] ( 773 ) HTML (1 KB)  PDF (1590 KB)  ( 765 ) 168 The suppressive effect of siILK on proliferation, migration and invasion of human extravillous trophoblastic cells LIN Yanyan, LIAO Tingting, JIN Jiaxi, CHEN jiajia, HUANG Xianping, XIANG Huiqiu, DONG Ke, XU Zhangye. Objective: To investigate the effects of integrin linked kinase (ILK) on the proliferation, migration and invasion of human extravillous trophoblastic cells (TEV-1). Methods: ILK-interferenced RNA was constructed and transfected into human TEV-1 cells, which was divided into experimental group (KD) and negative control group (NC); qRT-PCR and Western blot were used to detect the expression of ILK mRNA and protein. MTT assay, Transwell assay and Scarification test were used to detect the cell proliferation, migration and invasion. Results: Silenced ILK gene significantly reduced the ILK mRNA and protein expression levels of the TEV-1 cell (P<0.05), indicating that the transfection was successful. Silenced ILK gene significantly inhibited the proliferation, invasion (P<0.01) and migration of TEV-1 cells (P<0.05). Silenced ILK gene significantly decreased the mRNA and protein levels of MMP-9 and p-GSK3β in TEV-1 cells (P<0.01). Conclusion: These findings indicate that silenced ILK gene perturbs proliferation, migration and invasion may through down-regulated GSK3β and MMP-9 in TEV-1 cells. 2018 Vol. 48 (3): 168-171,177 [Abstract] ( 694 ) HTML (1 KB)  PDF (2573 KB)  ( 884 ) 172 Gene analysis and misdiagnosis of thyroid receptor β gene mutation in the pedigree with  thyroid hormone resistance QIAN Yanying, DAI Zhijuan, JIN Jian, ZHU Cong, ZHOU Yingying, WU Chaoming. Objective: To investigate the molecular genetics in one patients with resistance to thyroid hormone (RTH) in the pedigree and to analyze her general signs and misdiagnosis. Methods: With written informed consents obtained, the clinical data of the female patient with RTH and her family members were collected. Genomic DNA was extracted from the peripheral blood samples of the patient and her ten family members. The exons 3-10 coding region of thyroid hormone receptor β (TRβ) genes were amplified by PCR, and then directly sequenced. Results: In this pedigree, there were three RTHβ patients. The analysis identified a heterozygous mutant at exon 9 of TRβ (c.1234 A>G), which led to missense mutation in the proband as well as in her son and her mother. The proband presented with palpitation and weight loss, while her son had the characteristics of hyperactivity and the mother of the proband showed mild symptoms. Unfortunately, the proband and her son had been misdiagnosed for hyperthyroidism and underwent radioiodine therapy in another hospital. They may have to take levothyroxine for lifelong time. The mother of the proband had not been misdiagnosed because of the mild clinical manifestation. Conclusion: The RTH in the pedigree was caused by the TRβ mutation (A317T). It is of importance for doctors to know this disease better so that hormone ablation such as radioiodine therapy or thyroid surgery could be prevented. 2018 Vol. 48 (3): 172-177 [Abstract] ( 724 ) HTML (1 KB)  PDF (1273 KB)  ( 1144 ) 178 The effect of resveratrol on the expression of brain-derived neurotrophic factor in rats with irritable bowel syndrome ZHANG Jianbo, ZHU Naping, CHEN Jie, LIU Kaiping, YE Han, XU Wen, PAN Jianchun. Objective: To investigate the effect of resveratrol on depression- and anxiety-like behaviors, intestinal motility and visceral sensitivity in rats with irritable bowel syndrome. Methods: Forty-two SD rats were randomly divided into normal control group, chronic-acute combined stress (CACS) group, resveratrol group with different doses (10, 20 and 40 mg/kg), fluoxetine group and diazepam group. Twenty-two day CACS was used to established CACS model. The depression- and anxiety-like behaviors were evaluated by forced swimming test and marble-burying task. Intestinal motility and visceral sensitivity were measured by intestinal motility test and AWR test. The levels of brain-derived neurotrophic factor (BDNF) in hippocampus, frontal cortex, ileum and colon were measured by Western blot. Results: Compared with the normal group, the duration of immobility time of forced swimming and the number of marble buried was significantly increased (P<0.01 and P<0.01, respectively); the number of fecal output (P<0.01) in the intestinal motility test and the abdominal withdrawal reflex (AWR) score were also increased in CACS group (20 mmHg: P<0.05; 40 mmHg: P<0.05; 60 mmHg: P<0.01; 80 mmHg: P<0.01). The expression of BDNF in hippocampus (P<0.01) and the frontal cortex (P<0.01) were decreased, while the expression of BDNF in colon (P<0.01) and ileum (P<0.05) were increased in the CACS group, as compared with the normal control group. After 22-day treatment with resveratrol, the above data have been improved. Conclusion: Resveratrol ameliorated the behavior of IBS rats, which is likely to be related to the regulation of BDNF expression in the brain and gut. 2018 Vol. 48 (3): 178-184 [Abstract] ( 665 ) HTML (1 KB)  PDF (1675 KB)  ( 1121 ) 185 The treatment of percutaneous transhepatic gallbladder drainage for acute cholecystitis in high-risk patients CHEN Hui, ZHANG Xuming, HONG Zhong, ZHENG Peng, XIE Junqing, WANG Jisheng, ZHOU Bin, TONG Hongfei Objective: To summarize the experience in the treatment of ultrasound-guided percutaneous transhepatic gallbladder drainage (PTGD) sequential laparoscopic cholecystectomy (LC) in the elderly and high-risk patients with acute cholecystitis. Methods: The clinical data of 54 patients who underwent PTGD sequential LC between July 2013 and June 2016 were analyzed retrospectively. All the patients accepted LC after PTGD 1-2 weeks or 2-3 months determined by their family factors and physical status. Results: PTGD was successfully performed in all patients. Selective laparoscopic cholecystectomy was performed in 1-2 weeks or after 2-3 months. There was no significant difference between two groups in operative time, hospital stay, conversion to laparotomy and complications. Conclusion: PTGD is a safe, simple and effective procedure for treatment of elderly and high-risk patients with acute cholecystitis. If the patient’s conditions permit, early LC treatment was safe and feasible. 2018 Vol. 48 (3): 185-188,193 [Abstract] ( 729 ) HTML (1 KB)  PDF (2368 KB)  ( 861 ) 189 Factors affecting the treatment of humeral head replacement CHEN Chunhui, ZHANG Chuanxu, CAI Leyi, ZHENG Wenhao, TAO Zhenyu, CHEN Hua Objective: To explore the influencing factors of humeral head arthroplasty surgery effect.  Methods: From January 2010 to January 2014, 52 cases of proximal humeral comminuted fractures with treatment of the humeral head arthroplasty were selected. The 8 indicators that may affect humeral head arthroplasty surgery effectiveness were recorded and analyzed by logistic regression analysis, including age, sex, cause of injury, type of fracture, time from injury to surgery, time to start exercising after surgery, whether the patient is regularly followed-up and whether the patient is accompanied by osteoporosis. ASES scoring was applied to assess the outcome of the treatment. Results: Univariate analysis showed that there’s statistical difference (P<0.05) in age, time from injury to surgery, time to start exercising after surgery, whether the patient is regularly followed-up and whether the patient is accompanied by osteoporosis. There’s no significant difference (P>0.05) in gender, cause of injury and type of fracture. Multivariate logistic regression analysis showed that age (P<0.001, OR=1.127), time from injury to surgery (P<0.001, OR=1.104), time to start exercising after surgery (P<0.001, OR=1.064), and regular follow-up (P<0.001, OR=0.088) were the influencing factors, but osteoporosis was not (P=0.573, OR=1.102). Conclusion: Age, time from injury to surgery, time to start exercising after surgery and regular follow-up may be the chief factors affecting humeral head arthroplasty surgery effect, which should be taken into consideration for individualized treatment. 2018 Vol. 48 (3): 189-193 [Abstract] ( 703 ) HTML (1 KB)  PDF (1407 KB)  ( 994 ) 194 The influence factors on CT value of carotid atherosclerotic plaque in contrast enhanced qrterial phase YE Huichuang, KE Wenting, FAN Hengxin, CHEN Zhenjing, FAN Shufeng Objective: To investigate the influence factors and measuring CT value of carotid atherosclerotic plaques in the contrast enhanced arterial phase. Methods: Forty successive clinical diagnostic cases of unilateral or bilateral carotid atherosclerotic plaques (the maximum thickness >2 mm) confirmed by CTA and clinical data were randomly collected. The region of interest on the maximum level of the plaque without calcification and obvious artifacts was measured, the difference (∆Hu) of the CT value after plain scan and enhanced scan on the same level was calculated and the influence factors were investigated. Results: Of 61 carotid atherosclerotic plaques enrolled from the 40 cases, the CT value of CTA-phase in 26 plaques (42.6%) rose >10 Hu, 8 plaques (13.1%) declined >10 Hu, while the rest 27 plaques (44.3%) between the rise and decline. The median, maximum and minimum CT values of ∆Hu were 4.8 (-4.4, 19.3) Hu, 49.6 Hu, -55.3 Hu respectively. Conclusion: The concentration of the contrast medium in the arterial phase could affect the CT value of CAS plaque. 2018 Vol. 48 (3): 194-196,201 [Abstract] ( 743 ) HTML (1 KB)  PDF (2346 KB)  ( 1162 ) 197 The serum level of inflammatory cytokines IL-6 and IL-17A and its clinical significance in patients with anal fistula FANG Kexin, LIN Xiangtao, HUA Guohua, WANG Yongjie, ZHU Wangyu Objective: To investigate the serum level of inflammatory cytokines IL-6 and IL-17A and its clinical significance in patients with anal fistula. Methods: The serum levels of IL-6 and IL-17A cytokines of 49 patients with anorectal fistula and 48 healthy controls were detected by using cytometric bead array technology (CBA). The relationship between serum levels of cytokines and clinical characteristics was also analyzed. The pathological sections of anal fistula were stained with HE, and the bacteria in anal fistula was cultured according to the National Clinical Laboratory Procedures. Results: The serum level of IL-6 in patients with anal fistula was significantly higher than that in healthy controls (P<0.05). Moreover, pathological analysis indicated that the mucous membrane of anal fistula presents large number of lymphocyte infiltration in patients with high serum level of IL-6 and IL-17A. Patients with pus or pus germiculture positive had obviously higher IL-6 level than that in patients without pus or germiculture negative (P=0.008, 0.001). Conclusion: Patients with anal fistula have higher serum levels of IL-6 and IL-17A, which is associated with local mucous lymphocytic infiltration. Moreover, IL-6 level is correlated to anal fistula pus. Dynamic monitoring of the serum levels of IL-6 and IL-17A may produce clinical significance to evaluate the condition of patients with anal fistula. 2018 Vol. 48 (3): 197-201 [Abstract] ( 648 ) HTML (1 KB)  PDF (1395 KB)  ( 1067 ) 202 Role of ferulic acid in inhibiting the expression of MMP-1 and TIMP-1 in myocardium of rats with chronic heart failure by regulating activation of STAT3 protein WU Xiushao, WU Jing, LIU Zixia, CHEN Xujiao. Objective: To investigate whether ferulic acid could regulate the expression of matrix metalloproteinase-1 (MMP-1) and matrix metalloproteinase specific inhibitor-1 (TIMP-1) in myocardium of rats with chronic heart failure by inhibiting the activation of STAT3 protein. Methods: Fifty SD rats were randomly divided into 5 groups, sham operation group, model group, 25 mg/kg ferulic acid group, 50 mg/kg ferulic acid group and 100 mg/kg ferulic acid group. A rat model of chronic heart failure was made by coarctation of aorta. After 8 weeks of surgery, echocardiographic assessment was performed to detect the changes of cardiac function; collagen changes with Masson staining were observated; the expression of p-STAT3 protein level was detected by Western blot in myocardial tissue; the left ventricular myocardial expression of MMP-1 and TIMP-1 mRNA and protein were detected by RT-PCR and Western blot. Results: Compared with sham operation group in the model group, left ventricular end systolic diameter and left ventricular end diastolic diameter increased (P<0.05), ejection fraction decreased (P<0.05), myocardial collagen deposition obviously, the expression of p-STAT3 protein increased (P<0.05), expression of MMP-1 mRNA and protein increased (P<0.05), TIMP-1 mRNA and protein decreased (P<0.05). Compared with the model group, in the ferulic acid groups, left ventricular end systolic diameter and left ventricular end diastolic diameter reduced (P<0.05), ejection fraction increased (P<0.05), collagen deposition in the myocardial tissue decreased (P<0.05), p-STAT3 protein expression decreased (P<0.05), MMP-1 mRNA and protein expression decreased (P<0.05), TIMP-1 mRNA and protein increased (P<0.05). Conclusion: Ferulic acid may delay the progression of chronic heart failure by regulating the expression of MMP-1 and TIMP-1 in cardiac tissue of rats with chronic heart failure by decreasing the activation of STAT3 protein. 2018 Vol. 48 (3): 202-206 [Abstract] ( 650 ) HTML (1 KB)  PDF (1484 KB)  ( 1005 ) 207 The clinical significance of hTERC gene detection by FISH technique in the diagnosis and treatment of cervical lesions CHEN Lulu, WANG Yuli, ZHOU Qing, ZHOU Kai, ZHU Hua, HU Yan. Objective: To investigate the clinical significance of human telomerase RNA gene component (hTERC) detection in exfoliated cervical cells by fluorescence in situ hybridization (FISH) technique. Methods: hTERC gene amplification was tested by FISH in 235 patients at the First Affiliated Hospital of Wenzhou Medical University from December 2014 to October 2016. Retrospective analysis was made to explore the relationship between hTERC gene expression and the severity of histological and cytological abnormality. The association between hTERC gene amplification and postoperative pathological upgrade was analyzed in 61 patients who chose cervical conization surgery after FISH detection. Results: With the severity of cytological abnormality, hTERC gene abnormal amplification rate increased significantly (P<0.05). With the severity of histological abnormality, hTERC gene amplification rate and abnormal amplification rate increased significantly (P<0.05). The pathological upgrade rate after cervical conization surgery in patients with abnormal hTERC gene amplification rate was significantly higher than those with normal amplification rate (P<0.05). Conclusion: With the severity of cervical lesions, the expression of hTERC gene amplification rate increased. The pathological upgrade rate in patients with abnormal hTERC gene amplification rate was more likely to be higher than those with normal amplification rate. These results suggest that the hTERC gene detection with FISH technique has predictive value in the progression of cervical lesions. 2018 Vol. 48 (3): 207-210 [Abstract] ( 784 ) HTML (1 KB)  PDF (1259 KB)  ( 1262 ) 211 Impact of comprehensive geriatric assessment on the treatment outcomes of chronic heart failure in elderly patients and its influential factors LIU Xiaofang, LIANG Jingzhi, ZHENG Keda. Objective: To investigate the impact of comprehensive geriatric assessment (CGA) on the treatment outcomes of chronic heart failure (CHF), and analyze the influential factors of clinical efficacy in elderly patients. Methods: Totally 280 hospitalized patients with CHF in Fenghua People’s Hospital from January 2015 to April 2017 were recruited as participants, who were randomly divided into CGA group and control group with 140 cases each. The control group was given routine drugs, and individualized treatment was given under the guidance of CGA in the CGA group. The clinical effects of two groups were observed, and the influential factors were analyzed by logistic regression model. Results: There was no significant difference between the two groups (P>0.05) in general characteristics, the features of heart function and primary diseases at these baseline data. The total effective rate in CGA group was significantly higher than that in the control group (80.71% vs. 69.29%, P<0.05). The multivariate logistic analysis showed that the higher grade of heart function classification, larger number of complications, poorer quality of daily life, higher fall-down risk, poorer cognitive dysfunction and nutritional status were all significantly associated with the efficacy of elderly CHF (P<0.05). Conclusion: The influential factors for the prognosis of elderly CHF are numerous and complicated. The application of CGA will improve the clinical efficacy and prognosis by means of assessment of influential factors and appropriate individualized treatment under the direction of CGA. 2018 Vol. 48 (3): 211-215 [Abstract] ( 758 ) HTML (1 KB)  PDF (1264 KB)  ( 882 ) 216 The effect of CXCL12 on RANKL-induced osteoclastogenesis in RAW264.7 cells Lai Xianliang, Su Jia, Chen Ou. Objective: To investigate the effect of CXCL12 on receptor activator of NF-κB ligand (RANKL) induced osteoclastogenesis in RAW264.7 cells. Methods: RAW264.7 cells were cultured in vitro and induced by RANKL. CXCL12 was used to stimulate cells at concentration of 0, 25, 50 and 100 ng/mL. The influence of CXCL12 on cell proliferation was assessed using cell counting kit-8 (CCK-8). RAW264.7 cells were induced by RANKL in control group, while 50 ng/mL CXCL12 was used in CXCL12 group and 650 nmol/L AMD3100 was used in AMD3100 group. Osteoclasts were determined with tartrate resistant acid phosphatase (TRAP) positive staining and their morphology was observed. The expression of MMP-9, c-src, Cathepsin K in mRNA was analyzed using RT-PCR. The protein expression of MMP-9, p-src, Cathepsin K was detected using Western blotting. Results: CXCL12 had no effect on the proliferation of RAW264.7 cells; TRAP staining positive number was significantly higher in the CXCL12 group (P<0.05) and lower in AMD3100 group (P<0.05); CXCL12 can promote the expression of mRNA in MMP-9, c-src, Cathepsin K and the protein of MMP-9, p-src, Cathepsin K in RANKL-induced cells (P<0.05). Conclusion: CXCL12 can promote the differentiation of RAW264.7 induced RANKL cells into osteoclasts and improve the bone resorption ability of the cells, which mean CXCL12 maybe a target for osteoporosis therapy. 2018 Vol. 48 (3): 216-219,224 [Abstract] ( 762 ) HTML (1 KB)  PDF (2484 KB)  ( 1132 ) 220 The exploration of CRP/albumin ratio in the prediction of mortality in infants with severe pneumonia SUN Hengqiu. Objective: To evaluate the role of CRP, albumin and CRP/albumin ratio in predicting the outcome of severe pneumonia in infants. Methods: Altogether 73 patients with severe pneumonia were enrolled in this study and divided into survival group and death group according to 28-day mortality, and further divided into non-risk group, risk group and high-risk group according to the PCIS score. Serum CRP, albumin and PCIS score within 24 hours after admission were recorded. The CRP, albumin, CRP/albumin ratio were compared between survival group and death group. Results: The 28-day mortality was 27.4%. There was significant difference between survival group and death group in CRP, albumin and CRP/albumin ratio. Receiver operating characteristic curves showed the area under the curve of CRP/albumin ratio, CRP and albumin for 28-day mortality were 0.877 (P<0.05), 0.840 (P<0.05) and 0.841 (P<0.05), respectively. When the cut-off of CRP/albumin ratio was 0.5, the sensitivity and specificity were 85.0% and 77.4% respectively; when the cut-off of CRP was 16.5 mg/L, the sensitivity and specificity were 80.0% and 73.6% respectively. When the cut-off of albumin was 33.0 g/L, the sensitivity and specificity were 77.4% and 75.0% respectively. Conclusion: The CRP/albumin ratio, CRP and albumin were independent predictors of 28-day mortality in infants with severe pneumonia. The CRP/albumin ratio was better than CRP and albumin alone. 2018 Vol. 48 (3): 220-224 [Abstract] ( 715 ) HTML (1 KB)  PDF (1281 KB)  ( 967 ) 225 Preventive effects of comprehensive nursing care in elderly patients with high risk of osteoporotic fracture Zhang Rongli, Zhu Lihua Objective: To discuss the preventive effects of comprehensive nursing care in high-risk fracture patients with osteoporosis. Methods: Totally 120 elderly patients with high risk of osteoporotic fracture were selected by convenient sampling and were equally divided into interventional and control group. The control group received routine community health education, interventional group received comprehensive nursing care. After experiment, two groups were followed up in 12th and 24th month. Results: After intervention, the score of osteoporosis knowledge of the interventional group has been improved, statistically different from that of the control group (P<0.05). The result of osteoporosis health belief Scale showed a significant difference (P<0.05) between the two groups in regards of munity, seriousness, benefit of calcium uptake and so on. The result of the high-risk behavior showed a significant difference (P<0.05) in respects of solarizing, uptake of bean products, vitamin D and calcium. The incidence rate of tumble and fracture showed a significant difference (P<0.05) between the two groups in the 24th month of follow-up after intervention. Conclusion: The comprehensive nursing care can enrich the knowledge, enhance the health belief, improve high-risk behavior and has better preventive effect of tumble and fracture in the elderly patients with osteoporosis. 2018 Vol. 48 (3): 225-229 [Abstract] ( 718 ) HTML (1 KB)  PDF (1269 KB)  ( 1152 )

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