温州医科大学学报
 
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2018 Vol. 48, No. 1
Published: 2018-01-25

 
 
1 Comprehensive mapping of immunodominant B-cell epitopes and T-cell epitopes enriched areas of sperm associated antigen 9
Mao Chenchen, Ye Sisi, Zhang Teming, Han Zheng, Xue Xiangyang, Shen Xian
Objective: To predict the B-cell epitopes and T-cell epitopes enriched areas of sperm associated antigen 9 (SPAG-9). Methods: On account of the full-length sequence of SPAG-9, using bioinformatics software including the hydrophilic plot technique, polarity parameters, surface probability, antigenic index, and the secondary structure, along with its flexible regions and transmembrane region analysis, the B-cell epitopes of the SPAG-9 protein were predicted. Antigenic index further calculated to determine the target epitopes. Meanwhile, SYFPEITHI, NetCTL and IEDB were used to predict HLA-A2 and HLA-A24 restricted CTL epitopes. Finally, B cell epitopes and CTL epitopes were combined to predict the SPAG-9 epitope enriched areas. Results: The predicted B-cell epitopes of the SPAG-9 might exist in N-terminal of amino acid sequence: 197-201, 236-243, 249-253, 291-299, 306-312, 324-327, 332-337, 348-352, 488-494, 539-542, 546-550, 565-573, 699-702, 802-807, 869-873, 875-880, 882-887, 1173-1178, 1195-1200. The HLA-A2 restricted CTL epitopes which scored high of all the three methods were peptides about 19-27, 49-57, 50-58, 56-64, 343-351, 381-389, 413-421, 447-455, 521-529, 551-559, 665-673, 733-741, 837-845, 926-934, 950-958, 964-972, 998-1006, 1016-1024, 1094-1102, 1140-1148, 1150-1158 and the HLA-A24 restricted CTL epitopes were peptides about 31-39, 137-145, 517-525, 943-951. Combine the above results, the peptides about 332-352 (SAENEEKSEVQAIIESTPELD), 551-573 (SIWQFFSRL FSSSSNTTKKPEPP) might be the epitopes enriched areas of SPAG-9. Conclusion: Bioinformatics prediction shows that the peptides about 332-352 and 551-573 might be the epitopes enriched areas of SPAG-9. 
2018 Vol. 48 (1): 1-6 [Abstract] ( 843 ) HTML (1 KB)  PDF (1365 KB)  ( 980 )
7 Analysis and predication of B cell epitopes of programmed cell death ligand 1
CHEN Xudong, CHENG Kai, LYU Kaiji, ZHANG Lifang, ZHU Guanbao
Objective: To predict and screen the B-cell epitopes of the programmed cell death ligand 1 (PD-L1), in the hope of being used in the research of antagonist to block the protein-protein interaction between the transmembrane protein programmed cell death protein 1 (PD-1) and its ligand. Methods: Base on the full-length amino acid sequence of human and mouse PD-L1, the B-cell epitopes of the PD-L1 were predicted by bioinformatics method, including the secondary structure, the hydrophilic plot technique, polarity parameters, surface probability, and the antigenic index. Along with molecular modeling and functional mapping, antigenic index calculation was further taken as a standard to determine target epitopes. The amino acid sequence alignment of the predicted B epitopes of PD-L1 was blasted with other four kinds of experimental animal PD-L1. Results: Human and mouse PD-L1 was a 33 kDa type I membrane protein consisting of 290 amino acids. Extracellular part of human and mouse PD-L1 was at 19-238 and 19-239 respectively. The predicted B-cell epitopes of human and mouse PD-L1’s which could be used in the research of antagonist to block the protein-protein interaction between PD-1 and PD-L1’s might exist in N-terminal of amino acid sequence: 41-46 (KFPVEK), 60-63 (EDKN), 71-75 (EEDLK) and 40-48 (RFPVERELDL), 58-63 (EKEDE), 72-88 (EDLKPQH) respectively. Homology analysis showed the amino acid sequence (41-50, 58-62, 71-80) which contains the predicted B-cell epitopes was highly conservative in different species. Conclusion: Bioinformatic prediction along with molecular modeling and functional mapping which concludes in three amino acid seuences each for human and mouse provides a theoretical basis for further study of antagonist to block the protein-protein interaction between PD-1 and PD-L1.
2018 Vol. 48 (1): 7-12,19 [Abstract] ( 735 ) HTML (1 KB)  PDF (1872 KB)  ( 1015 )
13 The role of JNK on regulating NLRP3 inflammasomes activation in human corneal epithelial cells
TAN Qiufan, CHEN Huaicheng, ZHU Yirui, ZHU Hanlei, ZHENG Qinxiang, CHEN Wei
Objective: To investigate the role of JNK (c-Jun N-terminal kinase) in hyperosmosis-induced NLRP3 (NLR family pyrin domain containing 3) up-regulation and IL-1β secretion in the human corneal epithelial cells. Methods: HCECs cultured in normal osmolar media (310 mOsm) were switched to 500 mOsm, with or without the JNK inhibitor SP600125 (20 μmol/L). The mRNA levels of JNK, NLRP3, ASC, caspase-1 and IL-1β were detected by RT-qPCR. Caspase-1 activity was tested by caspase-1 colorimetric assay kits. Cell apoptosis was tested by AOEB staining method. The protein expression of NLRP3 and IL-1β were detected by immunofluorescence staining. And the protein level of JNK, p-JNK, NLRP3, caspase-1, ASC and IL-1β were determined by Western blot. Results: Hyperosmosis-induced mRNA overexpressions of JNK1, JNK2, NLRP3, caspase-1, IL-1β, TNF-α, IL-8 and IL-6 were significantly inhibited by JNK inhibitor (P<0.05). Moreover, hyperosmosis-induced caspase-1 activity was decreased with JNK inhibitor treatment by caspase-1 colorimetric assay (P<0.001). JNK inhibitor was able to downregulate cell apoptosis by AOEB staining. The protein overexpressions of NLRP3 and IL-1β induced by hyperosmosis were reversed by JNK inhibitor with immunofluorescence staining assays. And the protein overexpressions of p-JNK, NLRP3, caspase-1 and IL-1β induced by hyperosmosis were also downregulated with the use of JNK inhibitor by Western blot (P<0.05). Conclusion: NLRP3 inflammasome activation and subsequent IL-1β secretion are key consecutive events in HCECs response to hyperosmotic stress, and JNK may play a pivotal role in the regulation of the inflammasomes activation and expression of inflammatory factors.
2018 Vol. 48 (1): 13-19 [Abstract] ( 846 ) HTML (1 KB)  PDF (1505 KB)  ( 1103 )
20 Over expression of SIAH1 induced apoptosis of breast cancer cells by up-regulating the level of Hrk
QIAN Liyong, YANG Zhiqiang, WEN Yuanyuan
Objective: To investigate SIAH1 and Hrk expression in breast cancer, analyze the relationship between expression and clinicopathological features, and explore the expression SIAH1 promoting apoptosis of breast cancer by up-regulating Hrk expression. Methods: In this study, the Hrk and SIAH1 protein expression were detected in 86 cases of breast invasive ductal carcinoma and 32 cases in normal tissue adjacent to breast cancer by immunohistochemical SP method. The expression of Hrk and SIAH1 were examined in MCF-10A (breast normal epithelial cell line) and MCF-7 (breast cancer cell line). In MCF-7, it was transfected with pcDNA3-myc-SIAH1 (SIAH1 overexpression plasmid) only, and co-transfected with Hrk siRNA and pcDNA3-myc-SIAH1 by liposome-mediated method, respectively. Then cell apoptosis was detected with Annexin V-FITC/PI reagent kit and Flow cytometry assay. Results: Immunohistochemical analysis showed that the expression of SIAH1 and Hrk were lower in breast invasive ductal carcinoma tissues in comparison with normal tissues adjacent to carcinoma. The expression level of SIAH1 and Hrk correlated with higher grade (χ2=8.403, 8.514, P<0.01) and lower TNM stage (χ2=6.731, 6.729, P<0.05) significantly in breast cancer, and both the expression was positively correlated (r=0.515, P<0.01). After overexpressing SIAH1, the mRNA and protein expression of SIAH1 and Hrk (P<0.05), and the number of breast cancer cells apoptosis increased (29.02%±3.31%, P<0.05). Co-transfection pcDNA3-myc-SIAH1 and Hrk siRNA (4.40%±0.61%), compared with separate transfection pcDNA3-myc-SIAH1 (29.02%±3.31%), breast cancer cells apoptosis rate dropped significantly (F=187.369, P<0.05). Conclusion: Overexpression of SIAH1 can induce apoptosis of breast cancer cells by up-regulating the level of Hrk.
2018 Vol. 48 (1): 20-25,33 [Abstract] ( 817 ) HTML (1 KB)  PDF (1622 KB)  ( 1115 )
26 Effects of DRD4 gene polymorphism on brain resting-state fMRI in Chinese children of attention deficit hyperactivity disorder
QIAN Andan, WANG Meihao, WANG Xin, LIU Huiru, LI Jiance, YANG Chuang
Objective: To investigate the influence of dopamine D4 receptor (DRD4) gene polymorphism on brain resting-state functional magnetic resonance imaging (rs-fMRI) in children with attention deficit hyperactivity disorder (ADHD). Methods: rs-fMRI data was acquired in carriers of the DRD4 4-repeat/4-repeat (4R/4R) allele (DRD4 4R/4R group, n=30) and carriers of a DRD4 2-repeat (2R) allele (DRD4 2R group, n=19) in children with ADHD. The imaging characteristics of rs-fMRI between two ADHD genotypes were compared by using the methods of regional homogeneity (ReHo) and functional connectivity. Results: Compared with ADHD with a DRD4 2R allele, ADHD with DRD4 4R/4R allele showed increased ReHo in the bilateral cerebellum posterior lobe, the bilateral lingual gyrus and the right temporal occipital lobe. The decreased ReHo regions were the left angular gyrus. DRD4 4R/4R group showed functional connectivity increased to the left angular gyrus in the right frontal lobe and the bilateral cerebellum lobe. The functional connectivity decreased regions included the bilateral occipital lobe and the left frontal lobe. Conclusion: ReHo values and functional connectivity magnitude of many brain regions are significantly different between two groups of children with ADHD with different DRD4 genotypes. These findings could advance the understanding of the effects of DRD4 gene variation on children with ADHD in resting state.
2018 Vol. 48 (1): 26-33 [Abstract] ( 776 ) HTML (1 KB)  PDF (1756 KB)  ( 1425 )
34 Clinical features and sleep-disordered breathing in the Pierre-Robin syndrome children
Lin Jing, Zhang Yichun, Ye Chuyuan, Cai Huilin, Yu Chenyi, Cai Xiaohong
Objective: To study the clinical features and sleep-disordered breathing (SDB) in children with Pierre-Robin syndrome (PRS) to provide accurate diagnosis and treatment of PRS. Methods: Fifteen infants with Pierre-Robin syndrome admitted to the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from December 2014 to June 2016 were recruited in this study. A summary of the clinical characteristics, complications, and investigation of the prognosis by out-patient review or telephone follow-up was performed. Seven PRS children finished pediatric sleep questionnaires at 3 months old and eight children did it at 12 months old. Polysomnography (PSG) was monitored in 1 child. Results: Fifteen cases had typical clinical features of PRS including micrognathia, high arch palate, glossoptosis, and 3 had cleft palate. Thirteen cases developed dyspnea, 8 developed lung infection or aspiration pneumonia, and 6 developed feeding difficulties. The results of questionnaire survey show that 8 cases were screened positive for SDB at 3 months old, while 5 at 12 months old. Overnight PSG was performed in 1 case at 3 months and showed evidence of moderate OSAHS. The child with moderate obstructive sleep apnea underwent mandibular distraction osteogenesis at 4 months old. Twelve cases chose conservative treatment. Two cases failed to follow-up. Among 13 follow-up children, 5 died. In the 12th month of follow-up, the average weight of 8 cases was (8.0±1.5) kg, the average height was (72.0±3.0) cm. Conclusion: The PRS in children is associated with high risk for sdb. Timely diagnosis and proper treatment in children with PRS may help prevent long-term adverse sequelae.
2018 Vol. 48 (1): 34-37 [Abstract] ( 735 ) HTML (1 KB)  PDF (1235 KB)  ( 1088 )
38 The effects of different alcohol consumptions on liver function and glucose metabolism in fatty liver patients
LU Dongdong, WU Jinming, Xie Min, Zheng Liyu
Objective: To compare the effects of different alcohol consumptions on liver function and glucose metabolism in fatty liver patients. Methods: The patients diagnosed with fatty liver from physical examination center, outpatient services and inpatient department, were divided into 3 groups: non-drinking group, modest-drinking group and heavy-drinking group according to the amount of alcohol consuming. Then, the details of patients’ information include general physical condition, liver function and glucometabolic index were compared. Results: A total of 75 non-drinking patients, 62 modest-drinking patients, 70 heavy-drinking patients were collected. The modest-drinking group had a significantly lower level of systolic blood pressure (SBP) compared with other two groups and a significantly lower level diastolic blood pressure (DBP) compared with heavy-drinking group (P<0.05). In the comparison of body mass index (BMI) and waist-hip ratio (WHR), the modest-drinking group was the smallest (P<0.05). The modest-drinking group had significantly lower alanine transaminase (ALT), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) and significantly higher high-density lipoprotein cholesterol (HDL-C) than those of other two groups (P<0.05). The modest-drinking group had significantly lower hemoglobin A1c (HbA1c) and homeostasis model assessment-insulin resistance (HOMA-IR), significantly higher insulin action index (IAI) than other two groups (P<0.05). Conclusion: Compared with the patients of non-drinking group and heavy-drinking group, the patients in modest-drinking group could 
benefit from blood pressure, BMI, WHR, liver function, cardiovascular system, insulin resistance and so on.
2018 Vol. 48 (1): 38-41,45 [Abstract] ( 733 ) HTML (1 KB)  PDF (1237 KB)  ( 1087 )
42 Circadian expression of connexin genes in mouse heart and its correlation with biological clock
CHAI Ying, ZHOU Ying, CHEN Xiaomin, TONG Maoqing
Objective: To examine the circadian expression of cardiac connexin genes in the mouse and its correlation with biological clock. Methods: After 2-week constant housing, the atrium and ventricle of wild and Clock mutant mice were sampled every 6 hours. The expressions of Clock genes (Per2 and Dbp), cardiac connexin genes (Cx40 and Cx43) were determined by the real-time quantitative PCR. Results: The mRNA levels of all examined genes showed substantial circadian rhythms in wild type mice. In clock mutant mice, Per2 and Dbp lost circadian rhythms in both atrium and ventricle, while Cx40 and Cx43 still had circadian rhythms but phase-shifted significantly, which were phase-advanced in atrium and phase-delayed in ventricle. The Cx40, Cx43 expressions were upregulated in ventricle. Conclusion: Cardiac Cx40 and Cx43 gene expressions shows clear circadian rhythm in mice and may be associated with the biological clock system.
2018 Vol. 48 (1): 42-45 [Abstract] ( 619 ) HTML (1 KB)  PDF (1268 KB)  ( 861 )
46 Expression of ABCG2 in ovarian mucinous tumors and its significance
ZHANG Qian, WANG Haiou, ZHANG Huxiang, ZHENG Feiyun
Objective: To investigate the expression of cancer stem cell marker ABCG2 in ovarian mucinous tumors and its clinical significance. Mehtods: The expression of ABCG2 was detected by immunohistochemical PV-9000 2-step method in 22 cases of ovarian mucinous cystadenoma, 19 cases of ovarian borderline mucinous tumors and 25 cases of ovarian mucinous adenocarcinoma. The relationship between clinicopathologic characteristics was analyzed. Results: The positive expression rate of ABCG2 in ovarian mucinous cystadenoma, ovarian borderline mucinous tumors and ovarian mucinous adenocarcinoma was 18.2%, 47.4% and 80.0%, respectively. The expression level and positive expression rate of ABCG2 in benign mucinous cystadenoma and borderline mucinous tumor, in benign mucinous cystadenoma and mucinous adenocarcinoma, in mucinous borderline tumor and mucinous adenocarcinoma had statistical difference (P<0.05). The expression of ABCG2 in mucinous adenocarcinoma was significantly higher in stage III-IV than that in stage I-II (P<0.05), and it was significantly higher in moderate and poorly differentiated cancer tissues than in well differentiated cancer tissues (P<0.05), but not correlated with patient age (P>0.05). Conclusion: Expression of ABCG2 may participate in ovarian mucinous tumors during the progression from benign to malignant. And the protein detection may have referential significance in the estimation of tumor malignant degree. 
2018 Vol. 48 (1): 46-48 [Abstract] ( 710 ) HTML (1 KB)  PDF (1246 KB)  ( 885 )
49 The effect of blood pressure parameters on the outcome of the patients with cerebral hemorrhage in the acute stage
FENG Liang, WANG Zhen, ZHANG Zheng, YE Zusen, NIU Xiaoting, HAN Zhao
Objective: To study the effect of blood pressure parameters on the outcome of the patients with cerebral hemorrhage in the acute stage. Methods: Patients admitted in the neurology emergency clinic of the First Affiliated Hospital of Wenzhou Medical University were collected within 6 hours after onset from December 2010 to January 2013. Their blood pressure was recorded, relevant information was collected and all the cases were followed up for 3 months after stroke. Non-conditional logistic regressions analysis was adopted as the statistical method. To study the factors which affected the outcome of the first 7 days and 3 months after stroke. Results: In total 66 cases were analyzed. Multiplicity showed that the patient’s age (P=0.01, OR=1.19, 95%CI=1.04-1.36), the volume of hematoma (P<0.001, OR=1.34, 95%CI=1.12-1.59), the highest systolic pressure in the first 24 hours (P=0.02, OR=1.10, 95%CI=1.02-1.20) and the average systolic pressure in the first 7 days (P=0.01, OR=0.79, 95%CI=0.66-0.94) were the four independent factors associated with the outcome on the seventh days. The ROC curve was used to screen the boundary value, suggesting that the range of blood pressure in 151.5-178.5 mmHg was beneficial to the patients in acute period. The factors of age and the basic NIHSS were the independent factors connected with outcome of 3 months. Conclusion: The highest systolic pressure within the first 24 h (P=0.03, OR=1.09, 95%CI=1.01-1.17) and the average systolic pressure in the first 7 days (P=0.01, OR=1.27, 95%CI=1.07-1.50) could be used as the independent predictors for the outcome of acute stage of patients with cerebral hemorrhage, and a suitable range of blood pressure was beneficial for the outcome of the disease. The parameters of blood pressure could not be used as independent predictors for the outcome of 3 months in patients with cerebral hemorrhage.
2018 Vol. 48 (1): 49-52 [Abstract] ( 721 ) HTML (1 KB)  PDF (1199 KB)  ( 829 )
53 Analgesic effect of resveratrol on peripheral pain in mice via regulating the concentration of Ca2+ and its mechanism
WANG Lin, PAN Jianchun
Objective: To explore whether Ca2+, especially L-voltage-gated Ca2+ channels and Ca2+/caffeine-sensitive pools are involved in a algesic, effects and mechanisms in resveratrol-induced antinociception. Methods: Tail flick test was used to assess allodynia in mice treated with resveratrol (10, 20, 40 mg/kg, p.o.) alone or the combination of resveratrol or MK801 (0.5 mg/kg, i.p.) or nimodipine (2.5, 5, 10 mg/kg, i.p.) or CaCl2 (25, 50, 100, 200 nmol per mouse, i.c.v.) or ryanodine (0.25, 0.5, 1, 2 nmol per mouse, i.c.v.) or EGTA (5, 15, 30 nmol per mouse, i.c.v.). Results: The results showed that resveratrol increased the tail flick latency in the tail flick test (P<0.01). The effect of resveratrol was strengthened by MK801 or nimodipine (P<0.05). Central administration of Ca2+, however, diminished the antinociceptive effect of resveratrol (P<0.05). In contrast EGTA or ryanodine administered centrally improved the antinociceptve effect (P<0.05). Conclusion: The effect of resveratrol was enhanced by MK801, nimodipine, EGTA and ryanodine, while central administration of Ca2+, reduced the antinociceptive effects of resveratrol. The findings suggest that resveratrol produces effects on antinociception through regulation of calcium channels and calcium/caffeine-sensitive pools.
2018 Vol. 48 (1): 53-56,62 [Abstract] ( 746 ) HTML (1 KB)  PDF (1387 KB)  ( 919 )
57 Modulation of TuRenQiaoWei decoction on activities and mRNA expressions of cytochrome P450 enzymes in rats
HUANG Aifang, WANG Chenxiang, ZHOU Xiaojie, JIN Hui, ZHOU Bin, YU Xuben, LIN Guanyang
Objective: To investigate the modulation of TuRenQiaoWei decoction on activities and mRNA expressions of cytochrome P450 (CYP450) enzymes in rats. Methods: Twenty SD rats were randomly divided into treatment group and control group. After treatment with TuRenQiaoWei decoction or water for 14 days, treatment group rats and control group rats were given the mixture of four probe substrates including phenacetin, bupropion, chlorzoxazone and midazolam. The plasma concentration of probes at a series of time-points were determined by liquid chromatography tandom mass spectrometry. The activities of CYP450s were evaluated according to the pharmacokinetic parameters of corresponding substrates. Real-time PCR was applied to assess the mRNA expression levels of CYP450s. Results: The pharmacokinetic parameters of phenacetin and midazolam from treatment group showed significant differences compared with control group, which indicated that TuRenQiaoWei decoction induced CYP1A2 and CYP3A1 activity. And no significant difference was found in pharmacokinetic parameters of bupropion or chlorzoxazone from treatment group and control group. In addition, treatment with TuRenQiaoWei decoction significantly up-regulated the mRNA expression of CYP1A2 and CYP3A1 whereas it had no impact on CYP2B1 and CYP2E1. Conclusion: TuRenQiaoWei decoction can up-regulate the activities and mRNA expressions of CYP1A2 and CYP3A1.
2018 Vol. 48 (1): 57-62 [Abstract] ( 647 ) HTML (1 KB)  PDF (1362 KB)  ( 990 )
63 The relationship between levels of vascular endothelial growth factors and effectiveness of neoadjuvant chemoradiotherapy on locally advanced rectal cancer
ZHU Shengyu, SHEN Lerong, YE Xianghua
Objective: To investigate the relationship between levels of serum vascular endothelial growth factors (sVEGF) and effectiveness of neoadjuvant chemoradiotherapy on locally advanced rectal cancer. Methods: Levels of sVEGF were measured by enzyme-linked immunosorbent assay (ELISA) before and after treatment in 58 patients with locally advanced rectal cancer. The relationship between levels of sVEGFs and response of neoadjuvant chemoradiotherapy was analysed. Results: Levels of sVEGF were significantly decreased after treatment (P<0.01). Levels of sVEGF were different among patients with different pathological grades (P<0.05). Conclusion: The change of sVEGF level is able to evaluate the effectiveness of neoadjuvant chemoradiotherapy on locally advanced rectal cancer.
2018 Vol. 48 (1): 63-66 [Abstract] ( 743 ) HTML (1 KB)  PDF (1197 KB)  ( 987 )
67 Effect of intraoperative insulation measures on hypothermia and surgical site infection in patients undergoing laparoscopic radical gastrectomy
HU Mei, HUANG Shubin, YU Huixi, LUO Junjun
Objective: To investigate the clinical effect of intraoperative measures on intraoperative hypothermia and postoperative surgical site infection in patients undergoing laparoscopic radical gastrectomy. Methods: A total of 194 patients who underwent laparoscopic radical gastrectomy at the Department of Gastrointestinal Surgery of the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from August 2015 to January 2017 were divided into two groups according to intraoperative insulation measures: the intraoperative insulation group and intraoperative non-incubation group. The body temperature changes at the beginning of the surgery 30, 60, 120, 180 minutes and the end of the surgery, the incidence of hypothermia and surgical site infection, the time of extubation and the postoperative hospital stay were evaluated between the two groups. Results: The intraoperative insulation group was (36.43±0.19) ℃, (36.35±0.26) ℃, (36.35±0.28) ℃ 
and (36.27±0.30) ℃ respectively at 60, 120 and 180 minutes and at the end of operation. The intraoperative non-incubation group was (36.29±0.28) ℃, (36.21±0.34) ℃, (36.27±0.30) ℃ and (36.14±0.28) ℃ respectively at 60, 120 and 180 minutes and at the end of operation, the difference was statistically significant (P<0.05). The incidence of intraoperative hypothermia in intraoperative insulation group (33.3%) was significantly less than the intraoperative non-insulation group (59.4%), showing statistical difference (P<0.05). The incidence of postoperative surgical site infection (4.3%) in the intraoperative insulation group was lower than that in the intraoperative non-incubation group (12.9%), with statistical significance (P=0.043). Conclusion: Intraoperative insulation for gastric cancer patients can reduce the incidence of intraoperative hypothermia and surgical site infection and promote postoperative recovery.
2018 Vol. 48 (1): 67-69 [Abstract] ( 761 ) HTML (1 KB)  PDF (1194 KB)  ( 880 )
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