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2017 Vol. 47, No. 11 Published: 2017-11-25   781 he role of A-G single nucleotide polymorphism in the L allele of the 5-HTTLPR gene of attention deficit hyperactivity disorder: based on resting-state cerebral function ZHANG Xiaoyan, LIANG Yan, DU Songmei, LIN Haixi, WANG Weiqian,CHEN Hong, LIU Huiru, WANG Meihao, ZHAO Yongzhong, YANG Chuang Objective: To study the differences of amplitude of low frequency fluctuation (ALFF) between genotypes of SLA and SLG with attention deficit hyeractivity disorder (ADHD) children using functional magnetic resonance imaging (fMRI) and the relationship between ALFF and behavioristic. Methods: Screened out the genotypes of SLA and SLG of ADHD children by PCR gene detection technology. And all of the ADHD children were scanned with fMRI scanner in the resting state, using ALFF to assess the resting state cerebral function. And the Conners children behavior questionnaire was used to assess the behavior status quo. Results: Compared with the SLG genotype, SLA genotype showed ALFF were increased in right superior frontal gyrus, left middle frontal gyrus, left insula, right limbic cingulate gyrus, and no brain region with decreased ALFF. The ALFF of left middle frontal gyrus was significantly negative correlative with impulse-hyperactivity ratings of conners children behavior questionnaire, and ALFF of right limbic cingulate gyrus was significantly negative correlative with impulse-hyperactivity ratings and conduct problem score. Conclusion: Compared with the SLG genotype, the SLA genotype shows more yauld cerebral functional activities, and tend to be more serious impulse-hyperactivity and conduct problem. 2017 Vol. 47 (11): 781-785 [Abstract] ( 821 ) HTML (1 KB)  PDF (1424 KB)  ( 917 ) 786 Effect of gossypol on hepatic fibrosis of nonalcoholic fatty liver disease in diabetic rats LI Yangyang, CHEN Hanbin, XU Feifei, WAN Li, CHEN Guorong, CHEN Lei, CHEN Sanmei, WANG Rongrong. Objective: To investigate the effect of gossypol on nonalcoholic fatty liver disease (NAFLD) and its mechanism. Methods: Thirty male Sprague-Dawley rats were divided randomly into 3 groups: normal control (NC) group, type 2 diabetes mellitus (T2DM) group, type 2 diabetes mellitus treated with gossypol (T2DMG) group. After fed with a high-fat diet, the rats were injected with low-dose streptozotocin (STZ) intraperitoneally to induce type 2 diabetes mellitus. The T2DMG group were given 15 mg•kg-1•d-1 gossypol by gavage for 4 weeks, followed by 15 mg•kg-1•d-1 dose of gossypol every week for 8 weeks. The morphology of the liver was observed under light and transmission electronmicroscopy. Masson staining were used to observe the fibrosis of liver tissue. The protein expression of α-SMA, 11β-HSD1, GR, NF-κB and TGFβ1 were assayed by immunohistochemistry. The mRNA expression of 11β-HSD1, GR, NF-κB and TGFβ1 from rats livers were assayed by semi-quantitative RT-PCR. Results: Compare with NC group, T2DM group hapatic fibrosis was obvious, α-SMA, 11β-HSD1, GR, NF-κB and TGFβ1 protein expression was significantly enhanced (P=0.001, 0.004, 0.001, 0.001, 0.002), GR and TGFβ1 mRNA expression was significantly increased (P=0.01, 0.001). Compare with T2DM group, T2DMG group liver fibrosis was significantly ameliorated, α-SMA, 11β-HSD1, GR, NF-κB and TGFβ1 expression was significantly decreased (P=0.009, 0.031, 0.002, 0.009, 0.027), 11β-HSD1, GR and TGFβ1 mRNA expression was significantly reduced (P=0.003, 0.038, 0.014). Conclusion: Gossypol exerts the capacity to relieve hepatic fibrosis from NAFLD, inhibiting the expression of 11β-HSD1, GR and NF-κB, TGFβ1 may be involved in it. 2017 Vol. 47 (11): 786-790 [Abstract] ( 832 ) HTML (1 KB)  PDF (1625 KB)  ( 788 ) 791 Detection of viral and atypical pathogens in children with lower respiratory tract infection by multiple PCR technique ZHANG Hailin, CHEN Xiaofang, LYU Fangfang, ZHONG Peipei, CHEN Bo, XU Zhi, DONG Lin. Objective: To understand the distribution of viruses and atypical pathogens in children’s lower respiratory tract infections (LRTI), and to investigate the clinical value of multiplex PCR technique. Methods: Nasopharyngeal secretions of 992 children under 5 years old who were hospitalized for LRTI in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University were collected, from January 2014 to December 2014. Direct immunofluorescence assay was used to detect respiratory syncytial virus (RSV), influenza virus A, influenza virus B, parainfluenza virus, adenovirus and Chlamydia antigen, and extract nucleic acid. Use multiple PCR method based on advanced fragment analysis to detect RSV, influenza A virus, influenza virus B, parainfluenza virus, adenovirus, human rhinovirus (HRV), bocavirus, human metapneumovirus, coronavirus, mycoplasma pneumonia and Chlamydia gene. Results: There were 662 males and 330 females, 587 were in <1 year old group, 287 were in 1-3 years old group, 118 were in 3-5 years old group, the median age was 10 month. By using multiple PCR method, nasopharyngeal secretions from 851 patients were detected at least one pathogen (85.8%), 224 detected two or more than two pathogens (22.6%). RSV and HRV accounted for the top two of all detected pathogens, and the positive rates of pathogens in different groups were 84.8%, 89.2% and 82.2%, there was no significant difference between the three groups (χ2=4.416, P=0.110). However, the mixed infection rates in <1 year old group and 1-3 year old group were significantly higher than 3-5 year old group (χ2=3.963 and 9.871, P=0.047 and 0.002). As for LRTI children, HRV can be detected of all ages, while RSV is mainly in infants. Compared with immunofluorescence, the consistency of the same pathogen detection is good (Kappa=0.615, P<0.01), but multiple PCR method was better for detecting other pathogens (58.37% vs. 41.53%, χ2=56.23, P<0.001). Conclusion: RSV and HRV are the first two of the major virus and atypical pathogens of LRTI in children under 5 years old in this region. Bocavirus, coronavirus, and human metapneumovirus can also be detected, mycoplasma was rare. Compared with the direct immunofluorescence method, multiple PCR is helpful in comprehensive understanding the etiology of LRTI in children. 2017 Vol. 47 (11): 791-795,800 [Abstract] ( 888 ) HTML (1 KB)  PDF (2565 KB)  ( 1473 ) 796 Effect of Xiangsha Liujunzi Pill on liver’s mitochondrial of rats with nonalcoholic fatty liver disease Lou Linjie1, You Yanting2, MA Ke2, Chen Zewei2, Sun Xiaomin2, Zhou Lin3. Objective: To observe the effect of Xiangsha Liujunzi Pill on liver’s mitochondrial in rats with nonalcoholic fatty liver disease. Methods: Under specific pathogen free (SPF) conditions, 24 male Sprague-Dawley (SD) rats were randomly divided into control group, high fat and high cholesterol diet group (HFD) and Xiangsha Liujunzi Pill (1.35 g•kg-1•d-1) treatment group. After 12 weeks, all rats were executed to get liver samples. Blood paramaters were analyzed by automatic biochemical. The copy number of mitochondrial DNA (mtDNA) and the activity of mitochondrial respiratory chain complex I, IV in liver samples was analysized with RT-PCR and UV spectrophotometer respectively. Results: Compared with control group, the hepatic wet weight and mass index (hepatic wet weight/body mass) increased significantly, levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in serum were significantly increased, and the copy number of mtDNA and the activity of complex I, IV expression were significantly decreased in HFD group. Compared with HFD group, the liver wet weight and quality index of rats were significantly reduced, levels of TC and LDL-C in serum were significantly increased, and the copy number of mtDNA and the activity of complex I, IV expression were significantly increased in Xiangsha Liujunzi Pill treatment group. The HE staining of liver showed that the livers sections of HFD group were abundant of macrovesicular fat droplets in hepatocytes and large quantities of ballooned hepatocytes in centrilobular parenchyma with infiltration of inflammatory cells and fibrosis. The livers sections of HFD group showed that the macrovesicular fat droplets decreased and the nucleus consolidation was reduced. Conclusion: Xiangsha Liujunzi Pill would has effect on nonalcoholic fatty liver disease. Xiangsha Liujunzi Pill can regulate the disordered mitochondrial respiratory chain complex in nonalcoholic fatty liver disease. 2017 Vol. 47 (11): 796-800 [Abstract] ( 860 ) HTML (1 KB)  PDF (1419 KB)  ( 1040 ) 801 Feasibility of using axillary reverse mapping technique in breast cancer surgery and oncological safety SHI Yong1,2, WEN Tao2, HUANG Kaiming2, WANG Ke2, ZHENG Jingyan2, LI Xiaokun1. Objective: To research the feasibility of axillary reverse mapping (ARM) in axillary lymph node dissection for breast cancer patients and the safety of the tumor. Methods: Thirty patients with modified radical mastectomy for breast cancer were treated with methylene blue solution for lymph node biopsy. The axillary lymph node dissection was performed and the lymph nodes were removed for histopathological examination. Results: The 30 patients with breast cancer, and 21 patients had successfully detected blue-stained lymph nodes, 9 cases had failed, and 25 had blue-stained lymph nodes. No obvious cancer metastasis was found. Excision of lymph nodes in 21 cases, no case of upper limb lymph node edema. Conclusion: It is feasible to carry out ARM using methylene blue solution injection method for lymph node tracing and localization, and the oncologically burden is safe. 2017 Vol. 47 (11): 801-804,809 [Abstract] ( 644 ) HTML (1 KB)  PDF (2541 KB)  ( 1000 ) 805 The role of ARK5 in carboplatin resistance in epithelial ovarian cancer cell PAN Jiexue, LIN Feng. Objective: To investigate the mechanisms of carboplatin resistance in epithelial ovarian cancer (EOC) cells, and provide preliminary exploration on improving the effect of chemotherapy in ovarian cancer patients. Methods: Cell counting kit-8 was used to evaluate the effect of carboplatin on the proliferation of SKOV3 and A2780 cells, meanwhile the expression of epithelial-mesenchymal transition related proteins E-cadherin and vimentin were detected by Western blot. Flow cytometry assay was used to detect the expression of CD133. In addition, knockdown of ARK5 was to evaluate the role of ARK5 in the carboplatin-induced epithelial-mesenchymal transition. Results: The IC50 of carboplatin in SKOV3 and A2780 was 2.824 µg/mL and 1.760 µg/mL, respectively. Furthermore, we also found that carboplatin could significantly induce  epithelial-mesenchymal transition in EOC cells. Carboplatin could increase the expression of ARK5, but not SNARK. Knockdown of ARK5 could attenuate the induction of epithelial-mesenchymal transition. Conclusion: ARK5 plays an important role in carboplatin resistance in EOC cells. Knockdown of ARK5 may reduce the carboplatin-induced epithelial-mesenchymal transition. 2017 Vol. 47 (11): 805-809 [Abstract] ( 679 ) HTML (1 KB)  PDF (1495 KB)  ( 772 ) 815 Diagnostic value of apparent diffusion coefficient in pelvic lymph nodes metastasis of cervical cancer DONG Ledan, XIAO Qinqin, XU Huazhi, YE Qiong. Objective: To study the diagnostic value of apparent diffusion coefficient in pelvic lymph nodes metastasis of cervical cancer. Methods: Thirty-six patients of cancer patients were selected from August 2015 to May 2017, who were examined with preoperative 3.0T MRI in the First Affiliated Hospital of Wenzhou Medical University. Total 169 cervical cancer pelvic lymph nodes were collected, of which 70 metastatic lymph nodes (metastatic group) and 99 non-metastatic lymph nodes (non-metastatic group). The difference of the ADC values, the longest diameter, the shortest diameter, the volume of lymph nodes and the short/long ratio of metastatic lymph nodes and non-metastatic lymph nodes were analyzed using Wilcoxon test. Results: There was no significant difference of ADC values of lymph node between metastatic group and non-metastatic group (P>0.05). The longest diameter, the shortest diameter, the volume of metastatic group were longer than non-metastatic group (Z=-7.94, -7.76, -6.82, P<0.001), and the short/long ratio of metastatic group was smaller than non-metastatic group (Z=-1.12, P<0.001). Conclusion: The ADC diagnostic value on the pelvic lymph nodes metastatic of cervical cancer is not significant. However, combined with DWI and T2WI images to measure the size of lymph nodes can improve the diagnostic accuracy of metastatic lymph nodes. 2017 Vol. 47 (11): 815-819 [Abstract] ( 645 ) HTML (1 KB)  PDF (1537 KB)  ( 833 ) 820 Analysis on macular pigment optical density in diabetic patients without retinopathy XU Lijuan, YU Rui, LYU Zhigang Objective: To compare macular pigment optical density (MPOD) between healthy Chinese people and type 2 diabetic patients without retinopathy using the one-wave length reflectometry and to evaluate the correlation of MPOD with serum lipid levels. Methods: Forty-one type 2 diabetes without retinopathy and 21 healthy controls were enrolled in this study. All subjects underwent blood tests which included glucose (GLU), glycated hemoglobin (HbA1c), cholesterol (CHOL), triglyceride (TG), low density lipoprotein (LDL), high density lipoprotein (HDL), creatinine (CR), blood urea nitrogen (BUN), retinol binding protein (RBP) and homocysteine (HCY). MPOD (including mean OD: mean macular pigment optical density and max OD: maximum macular pigment optical density) was measured with one-wavelength ophthalmoscope. Results: Mean OD of type 2 diabetes was lower than the normal controls [(0.15±0.03) du vs. (0.16±0.02) du, P<0.05]. Mean OD level was positively correlated with HDL (r=0.28, P<0.05). Conclusion: Mean OD in diabetic patients without retinopathy is lower than that of healthy controls, and MPOD may affected by HDL levels. 2017 Vol. 47 (11): 820-823 [Abstract] ( 650 ) HTML (1 KB)  PDF (1344 KB)  ( 870 ) 824 The establishment of biological reference intervals of prealbumin and cholinesterase in serum for children in Wenzhou LI Shanshan, NI Li, ZHU Lidan, XU Xiaoyuan, DING Hongxiang. Objective: To establish the biological reference interval of serum prealbumin and cholinesterase for children (infant to school-age children) in Wenzhou. Methods: Retrospective study was adopted. The data of healthy infant (3 months to 12 years old) who visited the Second Affiliated Hospital of Wenzhou Medical University during April 2016 to March 2017 was collected. The qualified persons were divided into 6 groups according to gender and age, namely 3 months-3 years old (male, female infant group), -6 years old (male, female preschool group), 12 years old (male, female school-age group). By eliminating outliers within each group, 1 441 cases get qualified. Statistical methods was applied in comparing PA and CHE levels among the groups, if there was no statistical significance (P>0.05), the two age groups were merged, otherwise they won’t be merged. Results: The children’s serum PA reference intervals were 114.9-231.3, 126.4-237.4 and 136.7-273.5 mg/L for 3 months-3 years old, 4-6 years old, 7-12 years old. The difference of serum PA between male and female with the same age groups had no statistical significance (P>0.05). PA level was positively correlated with age (r=0.497, P<0.01). The children’s serum CHE reference intervals were 7 014-14 900, 7 079-14 246, 6 709-13 163 U/L for 3 months-6 years old, 7-12 years old (male), 7-12 years old (female). In addition to the school age group, the difference of serum CHE between male and female with the same age groups had no statistical significance (P>0.05). CHE level was not correlated with age (r=0.017, P>0.05). Conclusion: According to the research, reference intervals of PA and CHE are established for children at different age groups in the laboratory and reference in assessing children’s nutritional status and liver function are provided for clinicians. 2017 Vol. 47 (11): 824-827 [Abstract] ( 656 ) HTML (1 KB)  PDF (1387 KB)  ( 975 ) 828 Clinical observation of different doses of astragalus compound on patients with spinal cord injury with Qi deficiency and blood stasis ZHENG Xiuxia, HUANG Baoying, LAI Zijian, ZHU Zuwei, ZENG Wei, LU Xuhui, CHEN Yuxin. Objective: To observe the clinical effect of different doses of astragalus membranaceus on the patients with spinal cord injury due to Qi deficiency and blood stasis in Buyang Huanwu Decoction, and to provide the basis for clinical medication. Methods: Ninty spinal cord injury with Qi deficiency and blood stasis syndrome cases from July 2015 to December 2016 in orthopedics department of the Affiliated Ningde Hospital of Fujian Medical University, according to the order of the patients entered the trial, and to the random number table, randomly divided into 3 groups. Each group based on basic treatment, in a week after injury for traditional Chinese medicine (TCM) treatment, last one month, each group use basic prescription Buyang Huanwu Decoction. Group A with astragalus 30 grams, group B with 60 grams and group C with 120 grams, evaluated TCM syndrome score, the degree of spinal cord injury, activities of daily living (ADL) ability before and after traditional Chinese medicine treatment, judging TCM main symptoms and treatment efficacy after traditional Chinese medicine. Results: Compared with the main symptoms of Qi deficiency syndrome, group C was better than that of group A and B, the difference was statistically significant (P<0.05), group B was better than group A, the difference was statistically significant (P<0.05). Compared with the main symptoms of blood stasis syndrome, group C was better than that of group A and B, the difference was statistically significant (P<0.05), group B was better than group A, the difference was statistically significant (P<0.05). There was no significant difference between group A and B in the degree of SCI classification; the difference between group C and B was statistically significant, and also group C and A (P<0.05). Compared with ADL, there was no significant difference between group A and B, but the difference between group C and B was statistically significant, and also group C and A (P<0.05). Conclusion: Astragalus membranaceus 120 grams group is better than 60 grams group and 30 grams group in the treatment of SCI patients with Qi deficiency and blood stasis syndrome in different doses of Buyang Huanwu Decoction, which has a certain degree of dependence on measurement. 2017 Vol. 47 (11): 828-831,835 [Abstract] ( 646 ) HTML (1 KB)  PDF (2499 KB)  ( 1008 ) 836 Clinical features of scrub typhus and early diagnosis of severe scrub typhus WANG Ruili, DAI Wei, CHENG Bihuan, CHEN Yongping, GONG Yuqiang. Objective: To explore the clinical characteristics of scrub typhus and analyze the risk factors of severe scrub typhus, which can provide reference for early diagnosis of severe scrub typhus. Methods: The clinical data of 81 cases of scrub typhus which came from the First Affiliated Hospitals of Wenzhou Medical University and the Second Affiliated Hospitals & Yuying Children’s Hospital of Wenzhou Medical University between January 2010 and August 2016 were retrospectively analyzed, then summarized the clinical characteristics of these patients. According to whether to stay in the ICU, all patients were dividived into two groups: severe scrub typhus group and non-severe scrub typhus group. Logistic regression was used to analyze the clinical features and laboratory tests of two groups, which can be used to identify factors associated with severe scrub typhus. Results: All patients showed different degree of fever and clinical symptoms of scrub typhus were various but lack of specificity; eighty-seven percent of the patients had eschar or ulcer; the reduction of eosinophil percentage and (or) count, elevation of alanine transaminase and aspertate aminotransferase laboratory tests were easeily to find. Logistic regression analysis found that there were significant differences, such as elevated white blood cell count (OR=1.401) and decreased platelet count (OR=0.087) between severe scrub typhus group and non-severe scrub typhus group. Conclusion: Eschar or ulcer was found in medical examination, the decreased of eosinophil percentage or count and increased of liver transaminase were found in laboratory tests in fever pantients are useful for the diagnosis of scrub typhus. Increased white blood cells and decreased platelets are the risk indicators related to severe scrub typhus. 2017 Vol. 47 (11): 836-839 [Abstract] ( 781 ) HTML (1 KB)  PDF (1424 KB)  ( 1074 ) 836 Imaging features of 67 cases of children’s congenital cystic lung lesions XIANG Qiangwei, PAN Zhihuan. Objective: To summarize the imaging features of 67 cases of children’s congenital cystic lung lesions, so as to diagnosis these disease timely. Methods: Sixty seven cases of congenital cystic lung lesions children were collected in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from January 2000 to January 2016. The clinical data and imaging findings were retrospectively analyzed. Results: For patients of bronchogenic cyst, 29 cases showed solitary cyst, 13 cases were found with multiple systs. For patients of congenital cystic adenomatoid malformation, 8 cases were found multiple air syst in unilateral single or multiple lung lobe, 4 cases were showed pulmonary high density shadow, 1 cases were found huge liquid-gas syst in left lower lobe. For patients of pulmonary sequestration, 3 cases were found of pulmonary high density shadow, 1 cases of CTA showed pulmonary arterio-venous fistula, 1 cases of CTA were showed honeycomb cystic in lower lobe of left lung. Conclusion: There are  no specific manifestations and signs in patient with congenital cystic lung lesions. If the imaging performance are solitary or multiple cavities, liquid, or gas-liquid cyst, the possibility of congenital cystic lung lesions may need to be considered. 2017 Vol. 47 (11): 836-839 [Abstract] ( 511 ) HTML (1 KB)  PDF (1424 KB)  ( 834 ) 840 Analysis of thalassemia gene mutation types in Wenzhou XU Qiyu, ZHOU Wu, QIAN Jingjing, ZHOU Cui. Objective: To investigate the gene mutation type and constituent ratio of thalassemia, so as to provide prenatal and postnatal care. Methods: Nine hundred and ninety one suspected thalassemia patients from the First Affiliated Hospital of Wenzhou Medical University and the Second Affiliated Hospital of Wenzhou Medical University were included. GAP-PCR method and reverse dot blot (RDB) method were adopted to detect 3 common gene deletions in α-gene mutation and 3 point mutation in α-gene mutation, 17 common mutation sites in β-gene mutation. Results: From 991 suspected thalassemia patients, 431 thalassemia cases were detected (43.49%), including 155 α-thalassemia mutations and 276 β-thalassemia mutations. Southeast Asia deletion--SEA/αα (accounted for 67.10%) was the major types of α-thalassemia mutations. For β-thalassemia patients, the hot spots of mutation was CD41-42 (accounted for 35.87%). α-thalassemia combined with β-thalassemia were detected in 7 patients. Conclusion: α and β-thalassemia in populations of Wenzhou are complex. In order to provide valuable information with genetic counseling and clinical therapy, it is important to strengthen genetic diagnosis. 2017 Vol. 47 (11): 840-843 [Abstract] ( 666 ) HTML (1 KB)  PDF (1544 KB)  ( 1210 ) 844 The diagnostic significance of positive thyroid autoantibody in the first trimester in intrahepatic cholestasis of pregnancy GAO Bo, LI Hongmei, SUN Jianli YANG Xuefang, SHEN Beibei.  Objective: To investigate the diagnostic significance of positive antithyroid antibody (ATAb) in the first trimester in intrahepatic cholestasis of pregnancy (ICP). Methods: Select 120 cases of ICP pregnant who delivery in Shaoxing Women and Children’s Hospital with complete data from January 2016 to December 2016 as observation group, randomly selecte 120 cases of healthy pregnant according to the same proportion and during the same period as control group. Free triiodothyronine (FT3), free thyroxine (FT4), thyroid stimulating hormone (TSH), thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TgAb) are respectively determined in both the first trimester and the time of delivery in our hospital. Then statistics the number of positive ATAb cases. Compare and analyze the data of the two groups. Results: The observation group are compared with the control group, TPOAb in the first trimester increased significantly (Z=-2.309, P=0.021), institutional delivery gestational age significantly earlier (t=-7.318, P<0.001), TSH and TPOAb increased significantly (t=3.558, Z= -1.991, P<0.05) Conclusion: There is a certain relationship between positive antithyroid antibody in the first trimester and the occurrence and development of ICP. It have a certain significance to monitor thyroid autoantibody in the first trimester for early detection of ICP. 2017 Vol. 47 (11): 844-847 [Abstract] ( 555 ) HTML (1 KB)  PDF (1369 KB)  ( 851 )

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