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2017 Vol. 47, No. 9 Published: 2017-09-25   625 Preparation and in vitro antitumor evaluation of curcumin-loaded micelles FAN Ziliang, JIN Binghui, XU Xiafang, Jiang Qiaoying, XU Helin. Objective: To prepare a novel polymer micelles encapsulating curcumin (CUR) and evaluate its anti-tumor effect on glioma in vitro. Methods: A novel polymer, undecenoic acid-grafted-ε-polylysine (ε-PLL-UNA), was synthesized, and its chemical structure was confirmed by 1H-NMR and FT-IR. The CAC value of ε-PLL-UNA polymer was also detected by pyrene fluorescence probe. CUR-loaded micelle was prepared by dialysis method using ε-PLL-UNA as materials, and its particle size and morphology were also studied under dynamic light scattering (DLS) and transmission electron microscope (TEM), respectively. Furthermore, in vitro drug release profiles from CUR-Micelles were explored by dynamic dialysis method. Finally, the cellular toxicity against C6 cells spheroids and the cellular uptake of CUR-Micelles were evaluated. Results: ε-PLL-UNA polymer was successfully synthesized and able to self-assemble into micelles above its CAC value of 0.19 g/L. CUR-loaded micelle had a mean diameter of (60.6±2.1)nm, and zeta potential of (28.2±5.6)mV, exhibiting the spherical shape determined by TEM. Drug loading content and drug loading efficiency for CUR-loaded micelle were high up to 12.22%±2.13% and 85.12%±3.64%, respectively. About 84% of CUR were released from the micelles in 48 hours. CUR-loaded micelle can promote the cellular uptake of its encapsulated CUR by C6 cells, displaying a significantly higher cytotoxicity against C6 cells. Besides, the growth of C6 cells spheroids was significantly inhibited by CUR-loaded micelle. Conclusion: CUR is efficiently encapsulated in ε-PLL-UNA micelles with a particle size of less than 100 nm, which improved the cellular uptake of C6 cells. The sustained-release of CUR from CUR-loaded micelle is also observed. More importantly, CUR-Micelles has superior growth inhibition against C6 cells spheroids. 2017 Vol. 47 (9): 625-630,636 [Abstract] ( 722 ) HTML (1 KB)  PDF (2611 KB)  ( 1229 ) 631 The feasibility of the best phase half-cycle reconstruction improve to image quality in 320-row bronchial artery CT angiography WANG Zhenzhang, GUO Xianzhong, SUN Houzhang, CAO Guoquan, MAO Dandan, ZHANG Zhao, ZHENG Xiangwu, CHEN Weijian Objective: To assess the feasibility of the best phase half-cycle reconstruction in improving detection capability of bronchial artery and bronchial-pulmonary arterial fistulas for unanticipated free- breathing patients using 320-detector row dynamic volume CT. Methods: Fifty-five patients with severe motion artifacts during bronchial artery CTA were included in the study, who were scanned with prospective ECG-gating 320-detector row dynamic volume CT. All images were reconstructed with the best phase technique using half-cycle reconstruction (HCR) and multi-cycle reconstruction (MCR) methods.Wilcoxon signed-rank test was used to compare the grade (1, 2, 3, 4) of bronchial artery quantitative and paired t-test was used to compare the average CT values, noise (N), single to noise ratio (SNR), and contrast to noise ratio (CNR). Results: The number of bronchial artery branches at all grades from 1 grade to 4 grade in HCR group were 164, 177, 170, 94, respectively. It’s significantly greater than that in MCR group which were 131, 114, 76, 39, respectively. The bronchial artery CT values and noise in HCR group were greater than in that MCR group (respectively 373.3±61.0 vs. 331.2±65.0, 17.3±2.4 vs. 14.0±2.3, P<0.001). The SNR was significantly lower with HCR (38.5±6.9) than that with MCR (48.4±10.6, P<0.001). The CNRs had no significantly difference in the main segment of bronchial artery between the two groups (P=0.322). Conclusion: The best phase half-cycle reconstruction can improve image quality of bronchial artery CTA in high heart rates with unanticipated free-breathin, increase the number of tiny bronchial artery and bronchial-pulmonary arterial fistula significantly. 2017 Vol. 47 (9): 631-636 [Abstract] ( 740 ) HTML (1 KB)  PDF (1411 KB)  ( 961 ) 637 Biological effect of pyruvate dehydrogenase complex E2 subunit on monocyte U937 by activating Toll-like receptor 4-NF-κB pathway YANG Zaixing, LIANG Yan, LIU Donghong, ZHANG Zhiyu, ZHONG Renqian. Objective: To explore whether pyruvate dehydrogenase complex E2 subunit (PDC-E2) may activate Toll-like receptor 4 (TLR4)-NF-κB pathway and induce secretion of tumor necrosis factor-α (TNF-α), interleukin-12 (IL-12) and TNF-related apoptosis-inducing ligand (sTRAIL). Methods: PDC-E2 was used to stimulate monocyte line U937, combined with HTA125 (an inhibitory antibody of TLR4) and PDTC (an inhibitor of NF-κB). The study groups included control group, PDC-E2 group, PDC-E2+HTA125 group and PDC-E2+PDTC group. TLR4 on U937 was detected by flow cytometry, activity of NF-κB was determined by EMSA, TNF-α, IL-12 and sTRAIL were measured by ELISA. Results: At 24 h after stimulation by 2, 10 and 50 μg/mL of PDC-E2, TLR4 expression on U937 cell was significantly increased. At 1 h after stimulation by 50 μg/mL of PDC-E2, NF-κB was markedly activated, while at 2 h, the activation of NF-κB was gradually decreased and at 4 h, the decrease was very significant. NF-κB activity was significantly decreased after HTA125 addition compared with that before. At 24 h after stimulation by 50 μg/mL of PDC-E2, supernant TNF-α and sTRAIL levels were significantly higher than that of control, while IL-12 level was of no significant difference. At 24 h and 48 h, the levels of TNF-α, IL-12 and sTRAIL were all significantly higher than that of control. The three cytokines were significantly lower after addition of HTA125 or PDTC than before. Conclusion: PDC-E2 can activate TLR4-NF-κB pathway, by which PDC-E2 stimulates the secretion of TNF-α, IL-12 and sTRAIL by monocyte. 2017 Vol. 47 (9): 637-641 [Abstract] ( 610 ) HTML (1 KB)  PDF (1350 KB)  ( 1015 ) 642 Analysis of clinicopathology and prognosis of childhood Henoch-Schönlein purpura nephritis with renal tubulointerstitial lesions HU Yunxia, HU Xiaohan, Zhang Yuhua, HE Xiaoqing, Chen Lihong, Yang Qing, Lin Yiyi, ZHUANG Jieqiu Objective: To investigate the clinicopathological characteristics and prognosis in childhood Henoch-Schönlein purpura nephritis (HSPN) with or without tubulointerstitial lesions. Methods: The clinical and pathological data from 93 children with biopsy-proved HSPN were collected retrospectively in the Second Affiliated Hospital & Yuying Children’s Hospital of Wenzhou Medical University from January 2005 to December 2013. The patients were grouped according to the degree of tubulointerstitial lesions, and relationship between their clinical, glomerular parameters, prognosis and tubulointerstitial lesions was analyzed. Results: Ninety-three cases were divided into 3 groups according to the degree of tubulointerstitial lesions, group 1 with no tubulointerstitial lesions (16 cases, 17.2%), group 2 with mild tubulointerstitial lesions (66 cases, 71.0%), and group 3 with severe tubulointerstitial lesions (11 cases, 11.8%). The serum creatinine level (P<0.05) and the mesangial matrix score (P<0.05) was significantly higher in group 3 than that in group 1, and creatinine clearance rates of group 3 was significantly lower than that in group 1 and group 2 (P<0.05). The balloon adhesion score, fibrous crescents score and glomerulosclerosis score of group 3 were significantly higher than that in group 1 and group 2 (P<0.05). Multivariate logistic regression analysis showed that the balloon adhesion was risk factor of tubulointerstitial lesions. In 86 cases with follow-up, 96.5% had clinical normal or mild abnormal urine, 3.5% developed to renal dysfunction, the prognosis of group 3 was significantly worse than that in group 2 (P<0.05). Conclusion: Some HSPN patients may present with tubulointerstitial lesions in pathology at early stage of disease, which is reliable indicator for patients to develop severe glomerular lesion, and the patients with poor prognosis in future. 2017 Vol. 47 (9): 642-647 [Abstract] ( 768 ) HTML (1 KB)  PDF (1268 KB)  ( 860 ) 648 Effect of Benazepril on expression of TGF-β1, α-SMA and NOX4 in renal tissues of rats with unilateral ureteral obstruction ZHAO Yanyun, XIANG Xielong, JIAN Yijuan, DONG Feixia Objective: To observe the effects of Benazepril on expression of TGF-β1, α-SMA, NOX4 in renal tissues of rats with unilateral ureteral obstruction (UUO) and investigate the mechanism of it in renal interstitial fibrosis. Methods: The male Sprague-Dawley (SD) rats (54 cases) were randomly divided into Sham-operated group, model group and Benazepril treated group, 18 rats in each group. The benazepril group was given 1.6 mg/kg intragastric administration 1 times a day, and the model group and the sham operation group were given 10 mL/kg 0.9% sodium chloride solution gavage 1 times a day, model group and the benazepril group were ligated left ureter to establish UUO models. At days 7, 14 and 21, six rats in each group were sacrificed. Their left surgery renal tissues were collected for pathological examination. The pathological changes of the obstruction renal tissue were examined by Masson staining. The protein and gene expression of TGF-β1, α-SMA, NOX4 was detected by immunohistochemical staining respectively and NOX4 was detected by RT-PCR. Results: Compared with the Sham-operation group, the renal interstitial fibrosis degree of rats in other groups was increased (P<0.05), and aggratated as the ureteral obstruction time prolonged. Compared with the model group, after the treatment, the renal interstitial fibrosis degree decreased significantly (P<0.05). Compared with the Sham-operation group, the protein expression of TGF-β1, α-SMA, NOX4 in other group increased (P<0.05). Compared with the model group, after the treatment by Benazepril, the protein expression TGF-β1, α-SMA, NOX4 was down-regulated in the renal tissue (P<0.05). Conclusion: Benazepril may effectively retard kidney interstitial fibrosis by reducing the expression of TGF-β1, α-SMA, NOX4 to inhibit inflammatory of kidney. 2017 Vol. 47 (9): 648-653 [Abstract] ( 627 ) HTML (1 KB)  PDF (2146 KB)  ( 871 ) 654 PARP-1 protein inhibitor attenuated the growth inhibition and apoptosis of human cardiomyocytes induced by TNF-α XIE Yuequn, WANG Lei, CHEN Linglong, XU Ying, YANG Zhitao, LU Zhongqiu. Objective: To study the effect of PARP-1 inhibitor (4-Aminonaphthalimide) on the apoptosis of human cardiomyocytes mediated by TNF-α. Methods: MTT assays were used to detect the IC50 concentration of TNF-α on human cardiomyocytes cell line HCM, this assays were also performed to analyze the effect of the treatment with IC50 concentration of TNF-α combined with different concentration of PARP-1 inhibitor on proliferation of the HCM cells. Flow cytometry was used to monitor the apoptosis of HCM cells treated with different concentrations of TNF-α with or without PARP-1 inhibitors; RT-PCR and Western blot were used to analyze the expression level of PARP-1 in HCM cells after treated with different concentration of TNF-α with or without PARP-1 inhibitor. Results: TNF-α could perform the inhibitory effect of proliferation and induced the apoptosis of HCM cells, TNF-α also induced the degradation of PARP-1 protein and up-regulated expression of PAPR-1 gene in HCM cells (P<0.05); The inhibit effect of proliferation and the apoptosis rate of HCM cells induced by TNF-α was decreased after the intervention of PARP-1 inhibitors (P<0.05). The mRNA expression level of PARP-1 gene was down-regulated and the difference between the intervention group and the control group was not significantly in protein level. Conclusion: The effect of cells growth inhibition and apoptosis induced by TNF-α on HCM can be attenuated by blocking the activity of PARP-1 protein and gene transcription. 2017 Vol. 47 (9): 654-659 [Abstract] ( 729 ) HTML (1 KB)  PDF (2144 KB)  ( 1126 ) 660 Establishment of logistic regression diagnosis model predicting malignant solitary pulmonary nodules YU Wei, YE Bo, XU Liyun, Wang Zhaoyu, Wang Shanjun, Cao Hanbo, Chen Zhijun, ZHANG Yongkui. Objective: To establish a logistic regression model for predicting the probability of malignancy in solitary pulmonary nodules (SPNs) and provide guidance for the diagnosis. Methods: The clinical data and computed tomography (CT) images of 212 patients with a clear pathological diagnosis of SPNs were retrospectively analyzed from Zhoushan Hospital Affiliated to Wenzhou Medical University were analyzed retrospectively, among which, benign SPNs were collected from January 2012 to December 2015, and malignant SPNs were collected from January 2013 to December 2013. To estimate the independent predictors of malignancy of SPNs, multivariate analysis was used. A logistic regression prediction model was subsequently created. Data from an additional 242 patients with pathologic diagnosis of SPNs were used to validate this logistic regression prediction model. Results: Fifty-eight percent of the nodules from 212 SPNs patients were malignant and 42% were benign. Logistic regression analysis showed that there were significant differences in nodule type, clear border, lobulation, spiculaion, pleural retraction sign between subgroups with benign and malignant SPNs (P<0.05). These factors were identified as independent predictors of malignancy in SPNs. In our model, sensitivity was 81.8%, specificity was 85.7%, positive predictive value was 88.2%, and negative predictive value was 78.3%. Conclusion: The prediction model established in this study can be used to assess the probability of malignancy in SPNs, thereby providing help for the diagnosis of SPNs. 2017 Vol. 47 (9): 660-665 [Abstract] ( 721 ) HTML (1 KB)  PDF (1235 KB)  ( 906 ) 666 Association analysis of the SOX10 polymorphism and Hirschsprung LUO Chunfen, LI Shengmiao, PAN Zhiwen, XU Jiachuan, LIU Zhengji, YU Linjun Objective: To determine the association between the variations of SOX10 gene and sporadic HD in Zhejiang province. Methods: Blood from 95 sporadic HD cases and 80 normal cases were collected. Direct sequencing and polymerase chain reaction amplification were used to analyze 4 exons of the SOX10 gene for mutations and polymorphisms. Results: In this study, 4 single nucleotide polymorphisms (SNPs) were identified. No SOX10 gene mutations was found with sporadic HD. Conclusion: There is no association between these 4 SNPs of the SOX10 gene and HD. The SOX10 gene may not be the susceptibility gene of HD. 2017 Vol. 47 (9): 666-669 [Abstract] ( 710 ) HTML (1 KB)  PDF (1396 KB)  ( 722 ) 670 Effects of single short-term sevoflurane general anesthesia on early postoperative intelligence development in children aged 0-3 years HUANG Mengmeng, NAN Ke, HAN Yuan, SHAO Lingxiao, HU Mengjie, NAN Yang, JI Bin, LIN Han, LI Jun Objective: To explore the effect of single short-term sevoflurane general anesthesia on early postoperative intelligence development in children aged 0-3 years. Methods: Gesell development scale was used to evaluate the neurological development (DQ) of 200 children aged 0 to 3 years old 1 day preoperatively and 3 months after operation. Another 200 normal children in the same age range were selected respectively as control group I and control group II which compared and contrasted with those in anesthesia group. Results: Compared with the DQ scores preoperative 1 day, the developmental age (motor, adaptive, verbal, social) of the four energy regions of children increased to varying degrees after 3 months of anesthesia (P<0.001). The difference of DQ scores of four energy regions 1 day preoperatively and 3 months after operation was not statistically significant (P>0.05). There was no significant difference in DQ scores of four energy regions between anesthesia group and control group (P>0.05). Conclusion: A single short-term sevoflurane general anesthesia has no significant effect on the postoperative DQ of Gesell development scales in children aged 0-3 years, and all energy regions are normal development with age. 2017 Vol. 47 (9): 670-673 [Abstract] ( 692 ) HTML (1 KB)  PDF (1281 KB)  ( 844 ) 674 The protective effect of salvianolic acid A against apoptosis in middle cerebral artery ischemia reperfusion model and its mechanism PENG Xiao, Fang Shiji, Zheng Liyun, Qiu Weiwen Objective: To investigate the protective effect of salvianolic acid A (Sal A) against apoptosis in cerebral ischemia-reperfusion model and its mechanism. Methods: SD rats were randomly divided into sham operation group (sham group), middle cerebral artery ischemia reperfusion model (MCA-IR) group (MCA-IR group), Sal A pretreatment and MCA-IR group (Sal A+MCA-IR group). MCA-IR was established in MCA-IR group and Sal A+MCA-IR group in which rats were pre-treated with 1.0, 2.5, 5.0 mg/kg Sal A via intraperitoneal injection daily for 1 week. The artery were reperfused for 24 h and rats were sacrificed. TUNEL was used to assayed the cell apoptosis, immunohistochemistry was implied to test the expression of cPLA2 and Western blot was undergone to analyzed p-Akt and total Akt. Immunohistochemistry was used to analyze the expression of cPLA2. Results: The apoptosis rate and MDA level in Sal A+MCA-IR group was lower, but SOD was higher than that in MCA-IR group (P<0.05). The expression of p-Akt in Hippocampus of MCA-IR model decreased, while the expression of cPLA2 increased significantly compared with control group. 1.0, 2.5, 5.0 mg/kg Sal A pretreatment reversed the decrease of p-Akt and increase of cPLA2. Conclusion: Sal A inhibits the apoptosis through decreasing the expression of cPLA2 and activating Akt pathway signaling in MCA-IR model. 2017 Vol. 47 (9): 674-676 [Abstract] ( 688 ) HTML (1 KB)  PDF (1340 KB)  ( 827 ) 678 Mechanisms and effects of transcription factor SOX7 on the biological activity of the prostate cancer PC-3 cells ZHENG Bin, WU Qiquan, ZHOU Kewen, WANG Gang, REN Yu, QI Honggang, ZHU Weizhi, WENG Guobin Objective: To investigate the effects and mechanisms of transcription factor SOX7 on PC-3 cells proliferation, cell cycle, cell migration and invasion. Methods: PC-3 cells were transfected with high-expressed SOX7 recombinant plasmid (pCDNA3.1-SOX7) and further detected the changes of cell proliferation, cell cycle, migration, invasion, and related protein expressions. Results: After transfection with pCDNA3.1-SOX7, significant inhibition effects was shown in the PC-3 cell proliferation and G1/S cell cycle arrest, which were accompanied with decreased expressions of cyclin D1 and E. Furthermore, transfection of pCDNA3.1-SOX7 markedly decreased the cell migration distances and invasion capacity, with a reduction of the mesenchymal markers (MMP-2, MMP-9 and N-cadherin), however, the epithelial marker E-cadherin was increased. Conclusion: SOX7 inhibits PC-3 cell proliferation, cell cycle, migration and invasion through regulation of its related protein expressions. 2017 Vol. 47 (9): 678-682 [Abstract] ( 653 ) HTML (1 KB)  PDF (1667 KB)  ( 828 ) 683 The relationship between coagulation function and prognosis in acute pancreatitis A Yu, YE Suzhen  Objective: To explore the relationship among coagulation function, D-dimer and prognosis in acute pancreatitis (AP). Methods: Two hundred and eight patients with AP and fifty healthy people (control group) in the First Affliated Hospital of Wenzhou Medical University from January 2016 to December 2016 were enrolled. AP patients were divided into mild group (MAP group) and severe group (SAP group). Prothrombin time (PT), part of the activation of prothrombin time (APTT), concentration of fibrinogen (FIB), D-dimer and platelet functions were compared. The relevance to acute physiology and chronic health evaluation (APACHE II) and Ca2+ were analyzed. Results: The PT and FIB in AP patients were significantly higher than that in the control group (P<0.01). The PT, FIB, D-dimer and APACHE II score in SAP group were significantly higher than that in MAP group (P<0.01). Pearson correlation analysis showed that PT, FIB, CRP and D-dimer were negative correlation with Ca2+ (r=-0.317, -0.249, -0.392, -0.362, P<0.01), PT and D-dimer were significantly positive correlation with the APACHE II score (r=0.270, 0.203, P<0.01). Conclusion: There is coagulation dysfunction in AP patients, mainly exogenous coagulation way, it’ s closely associated with the severity and prognosis of diseases. PT plus D-dimer is a better predictive value for AP. 2017 Vol. 47 (9): 683-686 [Abstract] ( 693 ) HTML (1 KB)  PDF (1231 KB)  ( 1272 ) 687 Diagnostic value of 3.0T high-resolution MRI in paramedian pontine infarction LU Yefen, QIU Weiwen, ZENG Tingting, CHEN Weijin, CHEN Jianjun, RAO Jie. Objective: To apply 3.0T high-resolution MRI (HRMRI) in the diagnosis of basilar atherosclerotic in paramedian pontine infarction (PPI). Methods: Consecutive 53 patients with PPI were performed MRA and HRMRI, compared the differences in assessing the basal artery stenosis between the two methods. The distribution, composition features and remodeling index of basilar artery atherosclerosis were observed using HRMRI, the correlation of basilar artery atherosclerosis and cerebral infarction were analyzed. Results: Nineteen cases of basal artery stenosis deteeted by MRA (35.84%) compared with 34 cases detected by HRMRI (64.15%), there were significant statistically differences (P<0.05). Forty-four cases of basilar artery atherosclerosis were detected by HRMRI (83.01%). The basilar artery atherosclerosis were mainly distributed in the front and left sites, the percentage of eccentric plaques was 90.91% and unstable plaque was 59.09%. Negative remodeling was found in 15 patients, intermediate remodeling in 12 patients, and positive remodeling in 17 patients. The basilar artery plaques at or near the origin of the penetrating artery in 13 cases. Conclusion: HRMRI is more sensitive to detect basilar artery stenosis compared with MRA. The basilar artery atherosclerosis mainly distributes in the front and left sites in PPI patients, and plaque instability is high incidence. Morphological analysis of basal artery plaque using HRMRI has high value in patiens of PPI. 2017 Vol. 47 (9): 687-689 [Abstract] ( 635 ) HTML (1 KB)  PDF (1264 KB)  ( 685 ) 690 Diagnostic value of susceptibility weighted imaging in cerebral vascular malformations of children LIN Xubo, WU Aiqin, ZHENG Wenlong, YANG Zhongfu, DUAN Weimin, YANG Yubing, CHEN Tongjing. Objective: To evaluate the diagnostic value of susceptibility weighted imaging (SWI) in cerebral vascular malformations of children. Methods: Thirty eight cases of cerebral vascular malformations of children underwent routine magnetic resonance imaging (MRI) and SWI examination, which included 11 cases of arterio-venous malformations (AVM), 8 cases of cerebral venous malformations (CVM), 14 cases of cavernous hemangiomas (9 cases of solitary and 5 cases of multiple) and 5 cases of intracerebral capillary telangiectasia (ICT). Seven cases performed routine gadopentetate dimeglumine (Gd-DTPA) enhanced MRI scan. The difference of SWI and conventional MRI sequences on detection of cerebral vascular malformations was compared. Results: Thirty eight cases of cerebral vascular malformations were well-displayed on SWI. The detection rate of conventional MRI in AVM, cavernous hemangiomas, CVM and ICT were 100.0%, 78.6%, 37.5% and 20.0% respectively. While the detection rate of SWI in them were all 100.0%. SWI was obviously superior to conventional MRI on demonstrating microbleed foci, draining veins and medullary veins of cerebral vascular malformations. Conclusion: Comparing with conventional MRI, SWI can clearly show more details and detect more small lesions of cerebral vascular malformations, and avoid the missed diagnosis. It has an important value for the diagnosis and differentiation diagnosis of cerebral vascular malformation in children. 2017 Vol. 47 (9): 690-692,694 [Abstract] ( 648 ) HTML (1 KB)  PDF (2392 KB)  ( 901 )

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