Home  |  About Journal  |  Editorial Board  |  Instruction  |  Subscribe  |  Messages Board  |  Contact Us  |  中文

Forthcoming Articles Current Issue Archive Advanced Search Read Articles Download Articles Email Alert

2016 Vol. 46, No. 12 Published: 2016-12-25   859 The influence of two vascular smooth muscle cell primary culture methods on cellular contractile phenotype ZHOU Changzuan, GUO Hangyuan, MENG Liping, JI Zheng Objective: To explore the difference between collagenase digestion method and explant-culture method to obtain vascular smooth muscle cells (VSMCs) primary culture on maintaining contractile phenotype. Methods: The 1st, 2nd, 4th, 8th, 12th generation of primary VSMCs were obtained via type II collagenase digestion and explant-culture method. VSMCs contractile phenotype protein markers, calopnin and smooth muscle actin-α (SMA-α), as well assecreted protein osteopontin (OPN), were detected by western blot between different generations. The migration and proliferation ability of VSMCs from two methods were detected by wound-healing assay and MTT assay, and the phenotype protein markers expression by immunofluorescence and western blot. Results: We found VSMCs obtained from enzyme digestion initially exhibited stronger SMA-α and calponin expression, lower OPN expression and suppressed cellular proliferation and migration compared with that from explant-culture method. However, we observed a positive correlation between cell generation and VSMCs dedifferentiation degree, and a stronger dedifferentiation trend in enzyme digestion cells after 8th generation. Conclusion: Type II collagenase digestion method shows advantages in acquiring primary cells rapidly and maintaining VSMCs contractile phenotype within 4th generation compared with explant-culture method. 2016 Vol. 46 (12): 859-864 [Abstract] ( 736 ) HTML (1 KB)  PDF (2768 KB)  ( 1021 ) 865 Propofol relapse induced by drug-related environmental cues WANG Benfu, YANG Xiaowei, LAI Miaojun, ZHOU Wenhua, LIAN Qingquan. Objective: To induce rat establishing propofol relapse behavior, conditioned cues was utilized. Methods: Rats received propofol self-administration 3 h session per day for a total of 14 days under FR=1 (fixed- ratio=1) schedule. Twenty-four rats well trained for propofol self-administration were divided into 3 groups (n=8): Relapse 1 day (R1), Relapse 14 days (R14) and Relapse 30 days (R30). After withdrawal, those rats reintroduce to training-chambers for consecutive 1 hour relapse testing induced by contextual and contingent discreet conditioned cues respectively. Results: Stable propofol self-administration was established after 14 days training sessions. Compared to inactive nose-poke response, the number of active nose-poke response increased significantly (P<0.01). Compared to active nose-poke response of R1, the number of active nose-poke response of R14 increased significantly after 14 withdrawal days (P<0.01). And the number of active nose-poke response of R30 also increased after withdrawal 30 days, but there was no statistic difference compared with that of R1 (P=0.057). The number of active nose-poke response of contingent discreet conditioned cues was strongly higher than that of contextual (P<0.01). Conclusion: Propofol relapse can be induced by drug-related environmental cues, and propofol has obvious characteristics of relapse. 2016 Vol. 46 (12): 865-868 [Abstract] ( 622 ) HTML (1 KB)  PDF (1566 KB)  ( 760 ) 869 Investigation of the adjusting effect of WNK1 kinase on Maxi K channel SHI Zhen, ZHOU Lina, ZHANG Yiqian, WANG Dexuan, ZHUANG Jieqiu. Objective: To investigate the adjusting effect of WNK1 kinase on Maxi K channel in HEK-293T cells. Methods: The DNA plasmid carrying WNK1 and Maxi K gene was transfected into the HEK-293T cells, to observe the distribution and total protein expression of Maxi K in the HEK-293T cells via immunoflu-orescence microscopy and Western blot. Results: Immunofluorescence microscopic study showed that Maxi K expression was markedly increased in experimental group compared with the control group. Western blotting analysis showed that total Maxi K protein level was markedly increased in the presence of experimental group 1 and experimental group 2 compared with the control group (P<0.01). The total Maxi K protein level was markedly increased in experimental group 2 compared with the experimental group 1 (P<0.05). Conclusion: WNK1 can upregulate total protein expression of Maxi K, and the effect is enhanced with the increasing amount of WNK1 DNA, showing that the effect was dose-dependent. 2016 Vol. 46 (12): 869-872 [Abstract] ( 659 ) HTML (1 KB)  PDF (1586 KB)  ( 725 ) 873 Effect of HSP27 gene silencing on apoptosis of SW480 cells induced by 5-FU and its mechanism HUANG Chongjie, LIU Changbao, HU Wanle, CAI Mao. Objective: To explore the effect and mechanism of 5-FU-induced apoptosis of colon cancer SW480 cells with HSP27 silenced by siRNA interference. Methods: Six groups of cells were used in this part of research, including Normal control group (Normal group), negative siRNA group (NC group), HSP27 siRNA group (siRNA group), 5-FU group, 5-FU with negative siRNA group (NC+5-FU group), 5-FU with HSP27 siRNA group (siRNA+5-FU group). HSP27-siRNA was transfected into SW480 cells by lipofectamine 2000. CCK-8 method was used to detect the siRNA targeted on HSP27 and 5-FU’s inhibition effect on SW480 cell proliferation. Early apoptotic rate was measured with flow cytometry technique, and Western blot analysis was applied to detect the expression of apoptosis-related protein of Active-casepase-3, Active-caspase-9 and Cytc. Results: Result detected by CCK-8 showed that the cell proliferation inhibition rate of siRNA+5-FU group was obviously higher than that of 5-FU group, which indicated that combined use of 5-FU and siRNA could significantly improve the inhibition rate (P<0.05). Result detected with flow cytometry (FCM) showed that both siRNA group and 5-FU group could induce SW480 cell apoptosis, and the combined use of 5-FU and siRNA could greatly improve the early apoptotic rate. Comparing the siRNA and 5-FU group with Normal and NC group, the difference had statistically significant (P<0.05). Western blot analysis showed that the expression level of Active-casepase-3, Active- caspase-9 and Cytc in combination group were higher than that of siRNA group and 5-FU group, the difference had statistically significant (P<0.05). Conclusion: HSP27-siRNA can significantly inhibit HSP27 expression in SW480 cells, and improve the expression of protein Active-casepase-3, Active-caspase-9 and Cytc, then enhance the apoptosis-inducing effect of 5-FU on SW480 cells, and reverse the drug resistance of SW480 cells to 5-FU. 2016 Vol. 46 (12): 873-877 [Abstract] ( 764 ) HTML (1 KB)  PDF (1755 KB)  ( 795 ) 878 Variability of distal femoral valgus resection angle in patients undergone total knee arthroplasty: the incidence of femoral bowing and its influence  WU Peng, LANG Junzhe, LIU Yangbo, CHEN Lei. Objective: To measure distal femoral valgus resection and determine influential factors in patients undergone total knee arthroplasty. Methods: Two hundred and twenty two patients planned to undergo primary TKA from February first 2012 to February first 2014 in the First Affiliated Hospital of Wenzhou Medical University were included. The clinical data of age, gender, height, weight, body mass index and the radiographic data of distal femoral valgus resection, femoral length, femoral neck length, femoral shaft-neck angle, femoral bowing angle and mechanical lateral distal femoral angle were recorded and analyzed. Results: The mean distal femoral valgus angle was 6.66°±2.06° (range: 2.78°-12.84°). The femoral bowing angle was the factor that showed the strongest correlation with this angle (r= 0.845, P<0.01). The mechanical lateral distal femoral angle and age showed only a weak correlation (r=0.377, 0.446, P<0.01), and other parameters showed no correlation (P>0.05). Apparent femoral bowing angle (>3° of bowing) was found in 84 (37.8%) cases. Cases with apparent femoral bowing had statistically significant higher potential cutting error than cases with non-femoral bowing when a 5° or 6° distal femoral valgus angle was chosen (P<0.05). Conclusion: Chinese races’ distal femoral valgus angles are mainly influenced by femoral bowing among femoral deformities in the coronal plane. Therefore, it is necessary to measure the distal femoral valgus angle preoperatively from coronal radiographs covering the whole femur. 2016 Vol. 46 (12): 878-882 [Abstract] ( 751 ) HTML (1 KB)  PDF (1641 KB)  ( 814 ) 883 The effects of alpha lipoic acid on the stability of the atherosclerotic vulnerable plaques Wang Ying, Wang En, Wang Feng, Luo Huarong, Li Weiling, Li Cai. Objective: To discuss the effects of alpha lipoic acid on the stability of the vulnerable plaques.Methods: Atherosclerotic vulnerable plaques model were set up by liquid nitrogen frostbite in 28 health male Japanese big ear rabbits. The rabbits were randomly divided into 2 groups: Alpha lipoic acid (α-LA) group and the control group. The 2 groups were given equal amounts of α-LA and normal saline injection 1 time every 2 days for 4 weeks immediately after establishment of atherosclerotic vulnerable plaques model. Four weeks after treatment, the AS plaque was observed under the light microscope and electron microscope. The expression of vascular cell adhesion molecule 1 (VCAM-1), intercellular adhesion molecule 1 (ICAM-1) and monocyte chemotactic protein 1 (MCP-1) were detected with immunohistochemical method before and after treatment. The serum malondialdehyde (MDA) was determined with the glucosinolates barbituric acid method, the serum superoxide dismutase (SOD) activity was determined with hydroxylamine method, and the expression of serum matrix metalloproteinases 9 (MMP-9), hypersensitive c-reactive protein (hsCRP) and oxidized low density lipoprotein (ox-LDL) were determined with enzyme linked immunosorbent assay (ELISA) before and after treatment. Results: There was expression of ICAM-1, VCAM-1 and MCP-1 protein on atherosclerotic vulnerable plaque. The positive cells number and staining intensity in α-LA group were significantly reduced than that in the control group. The serum SOD was obviously higher and MDA significantly lower in α-LA group 4 weeks after treatment (P<0.01). The expression of MMP-9, hsCRP and ox-LDL was significantly lower than that at the beginning of treatment in two groups (P<0.01), but the α-LA group decreased significantly compared with the control group (P<0.05). Conclusion: α-LA has the function of the resistance to oxidative stress and inflammation, thus it can protect vascular intima, and it can stabilize the atherosclerotic vulnerable plaques. 2016 Vol. 46 (12): 883-886 [Abstract] ( 858 ) HTML (1 KB)  PDF (1917 KB)  ( 1001 ) 887 Expression and significance of tumor necrosis factor-alpha and interleukin-6 in juvenile nasopharyngeal angiofibroma WU Xianmin, YE Fan, WU Peng, CHEN Xiaoyun, LIN Chang, LIAO Zhisu. Objective: To detect the expression of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) in nasopharyngeal angiofibroma (JNA) and to explore the role of TNF-α and IL-6 in the mechnism of the bone destruction in JNA. Methods: The expressions of TNF-α and IL-6 in JNA tissue of 20 patients and normal inferior turbinate tissues in 10 cases of persons were determined by SP method of immunohistochemistry. Results: The positive expression rates of TNF-α and IL-6 proteins in JNA both were 90.0%, significantly higher than those in inferior turbinate tissues which both were 20.0% (P<0.01). The expressions of TNF-α and IL-6 protein were no correlation with the clinical stage of JNA (P>0.05). Conclusion: The higher expressions of TNF-α and IL-6 in JNA tissues may be the influence factors involved in bone destruction of the JNA. 2016 Vol. 46 (12): 887-890 [Abstract] ( 645 ) HTML (1 KB)  PDF (1572 KB)  ( 730 ) 891 Correlative research on meteorological factors and incidence of Kawasaki disease in Wenzhou ZHOU Aihua, YUE Yayan, JIN Zengyou, PAN Lulu, ZHANG Yingying, WANG Dan, QIAN Yan, ZHANG Yuanhai, WU Rongzhou, CHU Maoping. Objective: To explore the correlation between meteorological factors and incidence of Kawasaki disease (KD) in Wenzhou. Methods: The medical records of 1 956 KD patients and meteorological factors in Wenzhou from January 2005 to December 2015 were retrieved. The correlation between the number of KD patients and the meteorological factors was studied. Results: The incidence rate of KD was high in spring and summer but low in winter. Amount of rainfall was a positive correlation factor on the incidence of KD (r=0.217, P<0.05), while atmospheric pressure and wind speed were negative correlation factors (r=-0.209, -0.652, P<0.05). Temperature and sunshine duration had no significant correlation with KD incidence (P>0.05). Conclusion: The results suggest that meteorological factors are correlated with the pathogenesis of KD. 2016 Vol. 46 (12): 891-895,900 [Abstract] ( 629 ) HTML (1 KB)  PDF (2690 KB)  ( 854 ) 896 Dynamic changes of TNF-α and gelatinase on murine viral myocarditis model RUAN Miaohua, WANG Dan, WANG Kai, ZHOU Aihua, CHU Maoping, CHEN Qi, QIAN Yan. Objective: To explore the dynarnic changes and its meaning of TNF-α and gelatinase on the murine viral myocarditis model. Methods: Six-week-old inbred male mice were randomly assigned to control (n=35) and myocarditis group (n=60). The myocarditis and the control groups were inoculated intraperitoneally with 0.1 mL 10-5.69 TCID50/mL CVB3 or vehicle (PBS) alone respectively. Eight mice were sacrificed at 4th day, 10th day, 21th day and 30th day after injection. The serum content of TNF-α was measured by ELISA. The myocardial levels of MMP-2 and MMP-9 were determined by Western blot, MMP-2 mRNA and MMP-9 mRNA were measured by RT-PCR. Results: Compared with the control group, the protein and mRNA expression of MMP-2 and MMP-9 in the myocardium and the serum contents of TNF-α were significantly increased in myocarditis group (P<0.05). Conclusion: The expression of TNF-α and its downstream production MMP-2 and MMP-9 are increased in mice with acute viral myocarditis, and it may be involved in the pathogenesis of viral myocarditis. 2016 Vol. 46 (12): 896-900 [Abstract] ( 605 ) HTML (1 KB)  PDF (1493 KB)  ( 802 ) 901 Effect of mitochondrial aldehyde dehydrogenase 2 genotype on cardio-protection in patients with coronary artery bypass grafting ZHANG Yujian, DAI Yiru, XIA Fangfang, LIU Le, XU Xuzhong. Objective: To investigate the clinical effect of ALDH2*1 allele on cardio-protection of patients with coronary artery bypass grafting (CABG). Methods: Forty eight patients undergone simple CABG were divided into 2 group: ALDH2*1 (n=29) and ALDH2*2 (n=19), according to the results of ALDH2 gene SNP504 detection. 24 hours after operation, blood sample were taken for the measurement of cTnI, Mb, CK-MB and MDA. Postoperative mechanical ventilation time, ICU length of stay and postoperative 24 h cardiac drug use score were recorded. Results: There was no difference in the patient’s data among two groups, including age, body weight, ejection fractions and operation time. ALDH2*1 carriers showed lower cTnI, Mb, CK-MB and MDA, and shorter postoperative mechanical ventilation time, ICU length of stay and postoperative 24 h cardiac drug use score. Conclusion: ALDH2*1 allele protects against myocardium injury during open heart surgery cardiopulmonary bypass. The mechanism may be related to ALDH2 enzymes metabolism of toxic aldehydes. 2016 Vol. 46 (12): 901-904 [Abstract] ( 679 ) HTML (1 KB)  PDF (1461 KB)  ( 787 ) 905 Application of compound sodium aescinate gel in extraction of impacted mandibular third molars YU Yueyuan, SUN Renyi Objective: To evaluate the effects of compound sodium aescinate gel on postoperative complications after impacted mandibular third molar extraction. Methods: Thirty-five healthy patients with bilaterally symmetric, horizontally impacted mandibular third molars were included in this randomized split-mouth study. After extraction, the patients were treated with compound sodium aescinate gel on one side (test group) and without it on the other side (control group). Postoperative pain, edema, and maximal mouth opening measurements were compared, and a paired t test was done with SPSS17.0. Results: Postoperative pain, edema and maximal mouth opening measurements presented statistical differences between the two groups (P<0.05) at 24 hours and 48 hours after surgery. The test group presented with milder postoperative complications than the control group. Conclusion: The postoperative application of compound sodium aescinate gel can reduce the severity of postoperative complications after the extraction of impacted mandibular third molars. 2016 Vol. 46 (12): 905-908 [Abstract] ( 689 ) HTML (1 KB)  PDF (1563 KB)  ( 1181 ) 909 The diagnostic value of resistance index for benign and malignant parotid tumors HE Fenyi, SITU Mingzhu, Objective: To study the diagnostic value of resistance index (RI) in detecting benign and malignant parotid tumors. Methods: Preoperative ultrasound blood flow signal characteristics was analyze and the RI in primary blood supply arteries of surgical pathological confirmed parotid benign pleomorphic adenoma (22 cases), adenolymphoma (14 cases) and malignant lymphoma (17 cases) was measured. Then, the RI threshold value of malignant parotid tumors using the receiver-operating characteristic curve (ROC curve) was detarmined, the qualitative diagnositic sensitivity and specificity for malignant paroted tumors were calculated. Results: ①90% (20/22) of benign pleomorphic adenoma tumor blood flow signals are between I-II level, 79% (11/14) of gland lymphoma tumor blood flow signals was between II-III level, 76% (13/17) of malignancy tumor blood flow signals was between III-IV level. And blood flow signal differences among three groups had statistical significance (P<0.05). ②with RI acuity 0.69 as a diagnostic standard, the diagnostic sensitivity for malignant parotid tumors was 82%, while the specificity was 70%. Conclusion: Benign and malignant parotid tumors blood flow signals are related to its pathological basis. By determining the RI of threshold value it is advantageous in differentiation between benign and malignant parotid tumors, especially for glandular lymphoma and high-grade malignant tumors which have relatively abundant blood supplies, and also for identifying low-grade malignant tumors and benign pleomorphic adenoma. 2016 Vol. 46 (12): 909-911,915 [Abstract] ( 651 ) HTML (1 KB)  PDF (2854 KB)  ( 753 ) 912 Effect of specific immunotherapy on serum levels of  indoleamine 2, 3-dioxygenase in children with allergic rhinitis ZHENG BO, WANG Miao, NI Liyan, ZENG Yi, LIU Fanli. Objective: To explore the effect of subcutaneous specific immunotherapy on levels of the serum indoleamine 2, 3 dioxygenase in children with allergic rhinitis. Methods: Fifty-one children with persistent moderate to severe degree allergic rhinitis were divided into SIT group and drug group according to the wishes of their families, of whom thirty-one children received SIT (SIT group) while twenty children treated with drug therapy (drug group). Serum IDO concentration of the two groups were tested before treatment and one year after treatment. Nose total nasal symptom scores (TNSS) and medication scores were recorded at each time point. Results: The serum IDO concentration of the SIT group before SIT and one year after SIT was (70.43±8.80) ng/mL and (78.78±6.28) ng/mL, with statistically significant difference (t=5.72, P<0.01), the serum IDO concentration of the drug group before treatment and one year after treatment was (70.17±8.32) ng/mL and (69.27±6.69)ng/mL, there was no difference within this two groups (t=1.05, P>0.05). After 1 year of SIT, the TNSS and  medication scores of the SIT group were reduced,the difference was significant (7.52±2.16, 4.84±1.21 vs. 4.10±1.96, 1.06±1.00, both P<0.01). After 1 year of drug treatment, the TNSS and medication scores of the drug group were also reduced (7.35±2.08, 4.60±1.76 vs. 4.35±1.23, 2.60±0.99, both P<0.01). There was no correlation (all P>0.05) between the serum IDO concentration and TNSS of two groups at each time point. Conclusion: SIT can obviously control the allergic rhinitis symptoms, resulting in reducing the use of drugs. The serum IDO levels of children with allergic rhinitis are increasing during SIT progress. IDO may play an important role in the process of immune tolerance mediated from SIT. 2016 Vol. 46 (12): 912-915 [Abstract] ( 597 ) HTML (1 KB)  PDF (1461 KB)  ( 712 ) 916 Minimal invasive treatment of coronal plane fracture of the capitellum humeri of teenagers with the help of C-arm X-ray machine LI Yu’an, ZHANG Jingdong, YI Xianhong, MAO Chenghuang. Objective: To evaluate the clinical outcome of teenagers’ coronal plane fracture of the capitellum humeri treated with minimal invasive treatment with the help of C-arm X-ray machine. Methods: Twenty-eight  teenagers with coronal plane fracture of the capitellum humeri were collected from January 2003 to June 2013, among them 15 were male and 13 were female, age ranged from 8 to 14, average age was 10.3. All patients underwent open reduction and internal fixation using compressive hollow screws inserted in a posterior-to-anterior direction with the help of C-arm X-ray machine. Elbow pain, range of motion, stability and function were evaluated using the Mayo Elbow Performance Index after surgery. Results: The mean follow-up period was 24.2 months (ranged 7-36 months). The mean Mayo Elbow Performance Index score was 92.5 (range 80-100) and the scores were excellent in 20 cases and general in 8 cases. All patients achieved anatomic bone union and no patient developed avascular necrosis or heterotrophic ossification. Conclusion: Teenagers’ coronal plane fracture of the capitellum humeri treated with minimal invasive treatment with the help of C-arm X-ray machine have the advantages of minimal invasion, firm fixation, easy to operate and fewer complications, which can achieve excellent clinical outcome. 2016 Vol. 46 (12): 916-918 [Abstract] ( 680 ) HTML (1 KB)  PDF (1506 KB)  ( 732 ) 919 A clinical analysis of otorhinolaryngologic complications induced by fish bone YE Fan, CHEN Jianfu, WU Xianmin, CHEN Huanqi, LIN Renyu. Objective: To investigate the clinical features, diagnosis and treatment of otorhinolaryngologic complications caused by fish bone. Methods: The diagnosis and treatment of 10 cases of otorhinolaryngologic complications caused by fish bone were retrospectively analyzed from December 2009 to December 2015 in the First Affiliated Hospital of Wenzhou Medical University. Results: Six cases were neck abscess (including 3 cases of diabetes mellitus and serious condition, 1 case of positive pus culture), 1 case was laryngeal obstruction, 1 case was vocal cord granuloma, 2 cases were painless neck mass. Nine patients were diagnosed by CT of the neck and (or) B ultrasound, another by electronic laryngoscopy. Ten patients were treated by surgery, including 9 cases of foreign body removal and the prognosis is good, 1 case of failure of the first operation did not remove the foreign body, the second relapse after successful surgery; all patients were followed up for at least 6 months, no recurrence. Conclusion: Otorhinolaryngologic complications caused by fish bone are relatively rare, preoperative imaging and ultrasound can help to confirm the diagnosis, active surgical treatment can be able to effectively solution resolve complications, so that patients get good prognosis. 2016 Vol. 46 (12): 919-922,926 [Abstract] ( 590 ) HTML (1 KB)  PDF (2877 KB)  ( 1012 ) 923 Characteristic analysis of clear cell renal cell carcinoma by color Doppler ultrasonography and contrast-enhanced ultrasonography WANG Xiaohua, PAN Yin, LUO Hongxia, LIN Yiyi. Objective: To explore the imaging features between color Doppler flow imaging (CDFI) and contrast-enhanced ultrasonography (CEUS) in clear cell renal cell carcinoma, and evaluate the performance characteristics. Methods: The features of CDFI and CEUS of 34 CCRCC confirmed by operation and pathology were retrospectively analyzed, the characteristics of CDFI and the enhancement and wash out in different phases of CEUS were analyzed. Results: ①The imaging features of CDFI in CCRCC could be classified as two types, 10 cases of the CCRCC were rich in blood and the blood flow were not rich and in 24 cases. ②Enhancement patterns of renal clear cell carcinoma could be performed differently, and they were divided into four models. Twenty-six cases of the CCRCC indicated contrast enhancement patterns of fast in and fast out; six cases indicated that fast in and slow out; one case indicated that slow in and slow out; one case indicated that slow in and fast out. The degree of enhancement was divided into three types. Twenty-five cases of the CCRCC performed as hyper-enhancement; five cases performed as iso-enhancement; four cases performed as hypo-enhancement. The enhancement shape were mainly performed as two types, fourteen cases as rim enhancement and twenty cases as nonhomegeneous enhancement. The performance characteristics of CCRCC in the imaging of CEUS were fast in and fast out, the peak intensity, rim enhancement, nonhomegeneous enhancement. The diagnostic accuracy of CCRCC was 52.94% by CDFI and was 82.35% by CEUS, where significant difference was observed (P<0.05). Conclusion: CEUS shows some characteristic enhancement features, can display tumor vascularity and blood perfusion feature more sensitively than CDFI, and the confidence can be improved by the application of CEUS combined with CDFI. 2016 Vol. 46 (12): 923-926 [Abstract] ( 724 ) HTML (1 KB)  PDF (1564 KB)  ( 1263 )

Copyright © Editorial Board of JOURNAL OF WEZHOU MEDICAL UNIVERSITY Supported by:Beijing Magtech