温州医科大学学报
 
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2016 Vol. 46, No. 3
Published: 2016-03-25
 
 
157 Effect of low frequency electrical stimulation on soleus muscle fiber type of hypoxia-hypercapnia rats
HUANG Shiyuan, JIANG Xianxun, SHEN Jie, WANG Xiaotong.
Objective: To explore the effect of low frequency electrical stimulation on transition of rats’ soleus muscle fibre type after the long term chronic hypoxia-hypercapnia exposure. Methods: Forty male Sprague Dawley rats were randomly divided into four groups: the Normal Control (NC), Hypoxia-hypercapnia (HH), Hypoxia-hypercapnia+Tie-up (HT) and Low-frequency electrical stimulation (LFES). The HH, HT and LFES were placed in a chamber with hypoxia-hypercapnia for 8 h/d, 4 weeks in all. After 2 weeks, 30 min, 30 Hz, 1:1 cycle electrical stimulation was applied in the LEFS, this procedure lasted 2 weeks. The HT were only tied up and placed with electrode plates. Hematoxylin and Eeosin staining was used to observe pathological change of soleus muscle. Soleus fiber composition was visualized with immunochemistry, qRT-PCR was used to detect mRNA levels of MHC-I, MHC-IIa and IIx. Proteins expression of MHC-I, ERRγ, PPARβ was tested by Western Blot. Results: Increased inflammation cells and MHC-IIa/IIx mRNA, decreased cross section area, MHC-I, PPARβ protein and MHC-I mRNA were found in the HH and HT group compared with the NC. Compared with the HH and HT respectively, inflammation cells and MHC-IIa/IIx mRNA level were decreased, while mRNA level of MHC-I and protein expression of MHC-I, ERRγ and PPARβ were significantly increased after LFES. Conclusion: Hypoxia-hypercapnia leads to soleus muscle transition of MHC-I into MHC-IIa/IIx. However, LFES may reverse the muscle fiber transition induced by HH. PPARβ and ERRγ may have participates in this process.
2016 Vol. 46 (3): 157-162 [Abstract] ( 908 ) HTML (1 KB)  PDF (2018 KB)  ( 918 )
163 Transplantation of microencapsulated islets combined with renal arterial reconstruction to alleviate the early stage renal pathology in diabetic nephropathy rats
XU Ziqiang, HE Yunqiang, FU Hongxing, XU Yanyan, CAI Yong, XIA Peng
Objective: To establish rat’s model of transplantation of microencapsulated islets combined with renal arterial reconstruction and observe the curative effect of this model on the renal pathology in the early stage for the diabetic nephropathy in rats. Methods: Rat model of diabetes established by injection of a single dose of streptotocin were randomized into diabetic nephropathy (DN) group, microencapsulated islets group and combination group. After 4 weeks, 24 h proteinuria and random blood glucose level of all rats were examined, and pathological changes of the kidney and renal artery were observed under optical microscope. Results: Compared with DN group, blood glucose level and 24 h proteinuria in combination group was significantly lower. Microencapsulated islets group and combination group significantly alleviated glomerulosclerosis compared to DN group. However, there was no significant difference in the glomerulosclerosis between microencapsulated islets group and combination group. In addition, the intimal thickness of renal artery between micro-encapsulated islets group and combination group was not obvious. Conclusion: Microencapsulated islets combined with renal artery reconstruction can improve early stage renal pathology in DN rats.
2016 Vol. 46 (3): 163-167 [Abstract] ( 676 ) HTML (1 KB)  PDF (1746 KB)  ( 942 )
168 Detection of the plasma miR-23a expression in systemic lupus erythematosus and its clinical significance
YE Lulu, FAN Chaofan, HUANG Xixi, GUO Gangqiang, ZHANG Huidi, ZHANG Lifang, LIN Qiaoai, XUE Xiangyang.
Objective: To explore miR-23a expression and its clinical significance in the plasma of systemic lupus erythematosus (SLE) patients. Methods: Plasma samples from 54 SLE patients, 16 rheumatoid arthritis (RA) patients and 20 healthy controls were collected. The small RNAs in these plasma samples were isolated and reversely transcribed. Using cel-miR-39 as the external reference, the levels of miR-23a expression were detected with real-time polymerase chain reaction (PCR) method. The correlation between the levels of miR-23a expression and the clinical pathological features of SLE and biological significance of miR-23a expression in SLE were further analyzed with statistical methods. Results: Our data indicated that the levels of miR-23a expression in the plasma of SLE patients, RA patients and healthy controls were significantly different (x2=39.199, P<0.001). The level of miR-23a in the plasma of SLE patients was statistically lower than that in RA patients and healthy controls (P<0.05). The area under the ROC (receiver-operating characteristic) curve (AUC) was 0.931 for discriminating between SLE patients and normal subjects and 0.884 for discriminating between SLE and RA patients and patients. The levels of miR-23a expression were set the cutoff values of 19.22 for healthy control and 30.98 for RA patients, the diagnostic sensitivity and specificity were 74.5%, 88.9%, and 73.3%, 86.3%, respectively. The analysis of the correlation between miR-23a expression and the clinical pathological features of SLE shown that the levels of plasma miR-23a expression had positively correlated with anti-ANA titers and negatively correlated with white blood cell count (P<0.05). Conclusion: Down-regulated of miR-23a expression in plasma of SLE may be involved in the SLE disease occurrence or development and can be used as a novel potential diagnostic biomarker for SLE.
2016 Vol. 46 (3): 168-173 [Abstract] ( 652 ) HTML (1 KB)  PDF (1592 KB)  ( 1012 )
174 Expression and clinical significance of ultra early stage biomarker blood β-actin in patient with acute cerebral infarction 
ZHANG Yaping, WANG Binda, LIU Chunyan, YE Zhinan, TANG Weiguo, WU Zhouna, HE Songbin.

Objective: To explore the expression level of blood β-actin and its clinical significance in ultra early stage of acute cerebral infarction. Methods: Eighty patients with acute cerebral infarction (ACI) were selected as cerebral infarction group, and they were divided into five subgroups by different periods (2 hours, 4 hours, 6 hours, 24 hours and 72 hours), 16 patients in each subgroup. Other 48 cases without cerebral infarction were selected as control group, including 16 cases of transient ischemic attack, 16 cases of cerebral hemorrhage and 16 cases of healthy contrast group. The content of blood β-actin was detected by enzyme-linked immunosorbent method and analyzed combined with clinical condition. Results: ①The content of blood β-actin in the cerebral infarction group was significantly higher than those in the control group (P<0.05). ②The diagnostic kit for ACI was determined at 2.10 ng/mL by ROC curve. It had high sensitivity and specificity. ③The level of blood β-actin in patients with acute cerebral infarction has certain correlation with the area of cerebra1 infarction as well as NIHSS scores. Conclusion: The elevated level of blood β-actin may be a practical biomarker of acute cerebral infarction in ultra early stage.
2016 Vol. 46 (3): 174-177 [Abstract] ( 802 ) HTML (1 KB)  PDF (1348 KB)  ( 1278 )
178 Correlation study of single nucleotide polymorphisms in macrophage migration inhibitory factor gene with the risk of abdominal wall endometriosis after cesarean section
LIN Rongrong, GU Hangzhi, ZHOU Xinxin, ZHENG YUNFEI
Objective: To investigate whether polymorphisms of macrophage migration inhibitory factor (MIF) in promoter region (rs2012133G/C, rs4822443A/G and rs4822446G/A) are associated with hereditary susceptibility of abdominal wall endometriosis after cesarean section. Methods: Sixty patients with abdominal wall endometriosis after cesarean section were selected as case group and 109 healthy volunteers were collected as control group. The genotypes were detected by polymorphism chain reaction and restriction fragment length polymorphism analysis (PCR-RFLP). Results: There were statistical differences in genotype frequencies and allele frequencies between the case group and the control group (P=0.032; P=0.031) in rs2012133G/C site, GG genotype may markedly increase the risk of abdominal wall endometriosis after cesarean section (OR=2.284, 95%CI: 1.200～4.345). While no significant statistical differences could be found in rs4822443A/G, rs4822446G/A sites (P>0.05). Conclusion: The results suggest that MIF rs2012133G/C polymorphism is associated with abdominal wall endometriosis after cesarean section, GG genotype is the risk genotype.
2016 Vol. 46 (3): 178-182 [Abstract] ( 683 ) HTML (1 KB)  PDF (1419 KB)  ( 818 )
183 The protection of VGX-1027 on myocardial ischemia-reperfusion injury in mice cardiac transplantation
DAI Juji, ZHENG Xiangtao, FU Hongxing, ZHOU Mengtao.
Objective: To explore the effect of VGX-1027 on myocardial ischemia-reperfusion (MI/R) injury in mice cardiac transplantation. Methods: Forty mice were randomly divided into 2 groups: cardiac transplantation group and cardiac transplantation+VGX-1027 group (70 mg/kg). The grafts were collected at 24 h after cardiac transplantation. Histopathology was observed with haematoxylin-eosin staining. CD3+T cell, CD4+T cell and CD8+T cell infiltrated into cardiac grafts were detected by immunohistochemistry. Expression of caspase-3, Bax and Bcl-2 were determined with Western blotting, and cytokines expression of TNF-α, IL-1β and IL-6 were measured with ELISA. Results: Compared with cardiac transplantation group, mice treated with VGX-1027 exhibited significantly lower degree of leukocyte and myocyte necrosis. The infiltration of CD3+T cell, CD4+T cell and CD8+T cell in grafts were reduced with the treatmet of VGX-1027. The expression of caspase-3 and Bax was inhibited in cardiac transplantation+VGX-1027 group while the expression of Bcl-2 was increased. Furthermore, VGX-1027 pretreatment resulted in a decrease in serum cytokines of TNF-α, IL-1β and IL-6. Conclusion: VGX-1027 can significantly reduce ischemia-reperfusion injury in mice cervical cardiac transplantation model.
2016 Vol. 46 (3): 183-186 [Abstract] ( 839 ) HTML (1 KB)  PDF (1669 KB)  ( 872 )
187 Protective effect of ambroxol on experimental atelectasis
ZHANG Yili, TU Junwei, ZHOU Xiuyun
Objective: To investigate the effect of ambroxol on experimental atelectasis. Methods: Eighteen male China experimental miniature pigs (CEMP) were randomly divided into three groups: contral group (C group), atelectasis group (M group) and ambroxol treatment group (T group), respectively (n=6). Ni-Ti alloys occluders were placed in the lower right main bronchus of M and T group animals with a bronchofiberscope after anesthesia. CEMP of T group were treated with high dose of ambroxol hydrochloride injection (30 mg/kg, q 8 h, respectively) after the occluders procedure was finished. The development of atelectasis was observed by computerized tomography scanning every three days after occluders were placed in. On the third day of atelectasis, bronchoalveolar lavage was carried out. The animals were killed at the end of experiment, dipalmitoylphosphatidylchol (DPPC), large aggregates (LA) and small aggregates (SA) from the bronchoalveolar lavage was measured using high performance liquid chromatography. SP-A from the bronchoalveolar lavage was measured using a sandwich ELISA technique. Histopathological changes of atelectasis were determined under a light microscope. Results: Typical pathology of atelectasis and computerized tomography imaging were observed in group M. Concentrations of DPPC, SP-A and rate of LA/SA in M group were significantly lower than that in C group, while concentrations of DPPC, SP-A and rate of LA/SA in T group were markly higer than those in M group. The area of atelectasis was shruken in T group compared to those in M group. Conclusion: These findings suggest that ambroxol has beneficial effect on experimental atelectasis. This may be the result that ambroxol can contribute to enhances pulmonary surfactant synthesis and reduce the conversion of LA to SA.
2016 Vol. 46 (3): 187-189 [Abstract] ( 651 ) HTML (1 KB)  PDF (1433 KB)  ( 881 )
190 Study on the expression of ADAM10 in colorectal cancer tissues and serum
ZHENG Jia, SHENG Xiancang.
Objective: To detect the expression of ADAM10 in colorectal cancer tissue and serum, analyze the relationship between colorectal cancer occurrence, development, and clinical pathological characteristics. Methods: Samples of 59 colorectal cancer tissues, 30 colorectal polyps tissues and 30 health human of normal intestinal mucosa tissue were collected to detect the expressions of ADAM10 with immunohistochemistry SP. ADAM10 expression in serum of the above three groups was detected with double antibody sandwich ELISA method. Results: The expression level of ADAM10 in serum and tissue in the colorectal cancer patients was significantly higher than that in colorectal polyps groups and normal control groups (P<0.001). ADAM10 expression levels were elevated in serum and tissue of the colorectal cancer patients with the increase of tumor Duke stage. The expression level of ADAM10 in serum and tissue of the colorectal cancer patients was no relationship with the sex, age, the location of the tumor, tumor size, histological type (P>0.05), and was correlated with the tumor differentiation, lymphoid node diversion, distant metastasize, Duke stage (P<0.01). Conclusion: Colorectal cancer patients has higher expression of ADAM10 in cancer tissue and serum, and related with clinical pathological characteristics of colorectal cancer. It has a certain clinical value in the diagnosis of colorectal cancer.
2016 Vol. 46 (3): 190-193,198 [Abstract] ( 846 ) HTML (1 KB)  PDF (2484 KB)  ( 940 )
194 Epidemiologic investigation on pelvic floor dysfunction among women in Wenzhou
CHEN Cong, LU Yi, PENG Jiwen, HU Yanjun, LIN Xiaohua, WU Xueqing
Objective: To investigate the epidemiology of pelvic floor dysfunction (PFD) of the women in Wenzhou, analyze the relative risk factors resulted in PFD and study the differences of the prevalence of PFD between rural areas and urban areas. Methods: A total of 986 women recruited randomly were performed questionnaire survey and gynecological examination. The data were analyzed by Univariate Chi-square test and the logistic regression. Results: The prevalence of pelvic organ prolapsed (POP), stress incontinence (SUI), fecal incontinence (FI) of the women in Wenzhou was 17%, 24.7% and 0.7% respectively. The multivariate logistic regress analysis of the risk factors of POP showed that vaginal delivery (OR 3.5), age>40 (OR 2.1) and diabetes mellitus (OR 3.4) contributed to the development of POP; The multivariate logistic regress analysis of the risk factors of SUI showed that chronic cough (OR 3.6), hypertension (OR 1.7), vaginal delivery (OR 2.6), BMI≥25 kg/m2 (OR 1.7), education level below junior middle school (OR 1.4) and age>40 (OR 1.4) contributed to the development of SUI. Conclusion: The prevalence rate of PFD shows a general trend of increase with age. The independent risk factors of PFD are vaginal delivery, age>40, diabetes mellitus, chronic cough, hypertension, BMI≥25 kg/m2, education level below junior middle school. It is necessary to enhance the health education about PFD for women in Wenzhou,so that to prevent PFD effectively and make progress of life quality of the women with PFD.
2016 Vol. 46 (3): 194-198 [Abstract] ( 702 ) HTML (1 KB)  PDF (1366 KB)  ( 1055 )
199 Clinical and pathological features of IgA nephropathy combined with hypertension in 88 cases 
CHEN Qianqian, HUANG Chaoxing
Objective: To study the clinical and pathological features of primary IgA nephropathy (IgAN) combined with primary hypertension in 88 cases. Methods: A retrospective case series of 88 patients who were diagnosed as IgAN by renal biopsy and diagnosed with primary hypertension by clinical in our hospital between January 1997 to June 2013. Results: The mean age of the 88 cases of IgAN with hypertension was (44.7±11.27) years. Their hypertension history (median 36 months) was longer than IgAN’s (2 months). Forty-eight cases had family hypertension history, accounting for 54.5%. Sixty-six cases (75.0%) showed asymptomatic urine abnormalities, followed by 10.2% nephrotic syndrome and 11.4% chronic nephritis. Among the 29 cases (33.0%) of renal dysfunction (eGFR<90 mL/min), there were 6 cases (6/9) with the low-gravity urine and 21 cases (21/29) with the high NAG enzyme urine. Among the 88 cases, 52 cases (52/87) had the left ventricular wall thickening and (or) left atrial enlargement change, and 76 cases (76/80) had hypertension retinopathy. The renal biopsy showed Haas 1～3 grade level together accounted for 87.5% of lesions, severe glomerular sclerosis accounted for 19.3%, cases of moderate to severe renal interstitial disease accounted for 34.1%, and renal small artery disease accounted for 78.4%. Conclusion: The clinical and pathological manifestations of IgAN combined with hypertension patients are mostly mild; the cases of low eGFR mostly complicated with renal tubular dysfunction; the renal biopsy shows most glomerular lesion is mild, whereas the renal tubulo-interstitial and renal artery lesion is severe.Some cases show that tubulointerstitial lesions and glomerular lesions do not parallel, and the renal small artery lesions are exist. In IgAN patients with hypertension, the blood pressure may be the main factor to affect the renal prognosis.
2016 Vol. 46 (3): 199-202 [Abstract] ( 667 ) HTML (1 KB)  PDF (1306 KB)  ( 1011 )
202 A clinical analysis of 20 cases of pediatric  special types airway foreign body 
FENG Bohai, CHEN Bobei, GAO Jinjian, HUANG Saiyu, XIANG Haijie, SHI Yunbin, CHEN Si, CHEN Ruru, WU Jueting.
Objective: To explore the effective treatment for pediatric special types airway foreign bodies. Methods: The treatment of 20 cases of pediatric special types of airway foreign bodies (such as: plastic cap, internal parts of the ball-point pen, milk teeth, pins, straws, whistle, glass marbles, metal pieces, plastic pieces) were retrospectively analyzed from January 2012 to May 2015 in the Second Affiliated Hospital of Wenzhou Medical University. Results: Twenty cases of pediatric special types airway foreign bodies were removed through bronchoscopy, no death occurred. Conclusion: Ideal surgical methods and appropriate foreign body forceps should be chosen for the treatment of pediatric special types of airway foreign bodies according to different situations, strain capacity and operation skills can reduce the complications and reduce mortality.
2016 Vol. 46 (3): 202-204 [Abstract] ( 771 ) HTML (1 KB)  PDF (1269 KB)  ( 901 )
205 Comparison of two different surgical approaches for the treatment of lateral malleolus fractures 
LYU Yangxun, YANG Lei
Objective: To compare the outcome of two fixed methods for the treatment of anklebone fractures (stage IV). Methods: The clinical data of 80 patients with anklebone fractures were collected between Jan 2010 to Jan 2014. Forty-eight patients were treated with lateral approaches. Thirty-two patients were treated with posterolateral approaches. The indexs of perioperation, pain score at 3 days postoperative, bone union time, the clinical outcomes and postoperative complications were statistically compared. Results: There were no statistical significance on operation time, perioperative blood loss, bone union time and pain score at 3 days postoperative (P>0.05). The function of ankle score in the postoperative group was significantly higher than that in the lateral group [(44.3±1.5) vs (41.2±1.0)], meanwhile, the incidence of abnormal gait was lower. There were 3 cases of postoperative complications in the postoperative group, it was significantly less than the 6 cases of the lateral group. Conclusion: The lateral approach shows superiority in treatment of anklebone fractures, however, the posterolateral approach has the advantages in stage IV.
2016 Vol. 46 (3): 205-208 [Abstract] ( 983 ) HTML (1 KB)  PDF (1506 KB)  ( 1612 )
208 Clinical analysis of decompressive craniectomy in children with posttraumatic massive cerebral infarction
YU Lisheng, LIN Jian, SHENG Hansong, ZHANG Nu.
Objective: To explore the clinical effect of decompressive craniectomy in children with posttraumatic massive cerebral infarction. Methods: Twenty-three children with posttraumatic massive cerebral infarction undergone decompressive craniectomy from January 2012 to April 2015 were retrospectively reviewed. Results: Therapeutic effect was analyzed after postoperative follow-up of 3 to 12 months. The outcome was good recovery in 7 cases (30.4%), moderate to severe disability in 7 cases (30.4%), vegetative state in 4 cases (17.5%) and death in 5 cases (21.7%). Conclusion: Poor prognoses always occur in children with posttraumatic massive cerebral infarction. The outcome may be better when early and sufficient decompressive craniectomy is applied.
2016 Vol. 46 (3): 208-210 [Abstract] ( 714 ) HTML (1 KB)  PDF (1293 KB)  ( 902 )
211 Distribution and drug resistance of pathogenic bacteria isolated from blood culture in outpatients
WU Qing, CHEN Lijiang, XU Chunquan, ZHOU Cui, ZHANG Xueqing, YU Fangyou, ZHOU Tieli.
Objective: To investigate distribution and drug resistance of pathogenic bacteria isolated from blood culture in outpatients, to provide reference for the clinical rational use of antibiotics. Methods: Blood samples were cultured by BacT/Alert 3D automated system, the identification of strains and the drug susceptibility testing were performed by VITEK 2 COMPACT system, the data were analyzed with Whonet 5.6 software. Results: Totally 1 221 strains were isolated from 7 774 blood culture specimens in outpatients, with the positive rate of 15.7%, there were 569 positive cultures after the repeated strains were get rid of. Among the bacterial strains ,the percentages of the gram-negative bacteria, gram-positive bacteria and fungi were 57.1% (325 strains), 41.7% (237 strains) and 1.2% (7 strains), respectively. Pathogenic bacteria resistance rate was relatively low, the positive rate of ESBLs in Klebsiella pneumoniae was 11.1%, resistance of methicillin-resistant Staphylococcus aureus isolation rate was 28.6%; separation of Enterobacteriaceae to carbapenem antibacterial drug resistance<1.0%, vancomycin resistant Staphylococcus aureus did not be found. Conclusion: The main pathogens of outpatient blood culture are Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae, and these bacteria are better sensitivity to commonly used antibiotics in clinical practice.
2016 Vol. 46 (3): 211-214 [Abstract] ( 715 ) HTML (1 KB)  PDF (1290 KB)  ( 847 )
215 Randomized trial of oxycodone hydrochloride prolonged-release tablets with or without amitriptyline for severe cancer pain patients with depression
WANG Jiangang
Objective: To investigate the clinical efficacy of oxycodone hydrochloride prolonged-release tablets with or without amitriptyline for severe cancer pain patients with depression. Methods: Thirty-four patients with severe cancer pain and depression were randomly divided into two groups. Oxycodone hydrochloride sustained-release tablets combined with amitriptyline were applied for 17 patients in treating group. Oxycodone hydrochloride sustained-release tablets without amitriptyline for cancer pain treatment as control group. All patients were observed  for a period of four weeks. The facial pain rating scale (FPS-R) and Hamilton rating scale for depression (HAMD17) were used to assess the clinical efficacy. The dose and adverse drug reactions of Oxycodone were recorded. Results: Pain relieved in each groups of the patients after treatment (P<0.01). It was more effective that depression improved in treating group, HAMD17 scores were significantly lower than that of pretreatment and the control group (P<0.01). The average daily dosage of oxycodone hydrochloride prolonged-release tablets for treating group was significantly lower than that for the control group during the fourth week (P<0.01) and the adverse reactions between two groups have no significant difference. Conclusion: Oxycodone hydrochloride prolonged-release tablets combined amitriptyline can effectively control severe cancer pain, improve depressive symptoms and quality of life for advanced cancer patients.
2016 Vol. 46 (3): 215-217 [Abstract] ( 738 ) HTML (1 KB)  PDF (1299 KB)  ( 1072 )
218 Modified urethral dilatation under direct vision of endoscope to treat the urethral stricture in 20 cases
YANG Yu, HUANG Hang, DENG Zhexian, XIE Hui, ZHANG Yan
Objective: To explore the method of the urethral under cystoscopy to treat the urethrostenosis and to evaluate the curative effect of this method. Methods: The therapeutic data of the urethrostenosis in 20 cases admitted to our hospital from September 2010 to September 2012 were retrospectively analyzed. Of them, 11 cases had the history of lower abdominal open operation and all of them were admitted to hospital because of acute urine retention and failure to indwell catheter and urethral dilatation. All patients were further dilated with cystoscopy under direct vision of 19.5 F cystoscopy after the ureteral catheter dilatation. 16 F and 18 F catheters were indwelled for 1-2 weeks after operation, then the conventional regular dilatation was performed. Results: Operations were successfully performed and catheter was indwelled in 20 cases. Of them, the conventional urethral dilatation could be smoothly performed under the blindness and urine could swimmingly urinate after catheter was removed postoperatively 1 week. Postoperative follow-up for 3 months-2 years. Five cases of swimmingly urination needn’t treat again, the rest of patients could smoothly undergo regular urethral dilatation. Conclusion: The endoscope dilatation technique for treatment of urethral stricture has better effect, and can avoid urethral false passage, especially for patients with acute urinary retention with lower abdominal open operation history and can avoid risks of bladder puncture fistulation and the intestinal perforation.
2016 Vol. 46 (3): 218-220 [Abstract] ( 686 ) HTML (1 KB)  PDF (1366 KB)  ( 1250 )
220 Application of phloroglucinol in the treatment of acute biliary colic
LI Ying, YU Fuxiang, YE Yuanyuan, LIN Weihong, ZHANG Ruodan, SUN Hongwei
Objective: To analyze the value of phloroglucinol in the treatment of acute biliary colic. Methods: Three hundred and sixty-eight patients with acute biliary colic were randomly divided into two groups, 236 as the treatment group and 132 as the control group. 120 mg phloroglucinol was added in 5% glucose solution through intravenous drip in the treatment group, while 10 mg anisodamine through the same way in the control. Both two groups used associated antibiotics. The anticolic efficacy was analyzed as well as the side effects. Results: One hour after treatment, the excellence rate of the treatment group was 69.1%, while total efficacy rate was 91.1% and the excellence rate of the control group was 53.0%, while total efficacy rate was 74.2%. There was significant difference between the treatment group and the control group (P<0.05). The incidence rate of the side effects in the treatment group was 0.9%, while 95.4% in the control. There was significant difference between these two groups (P<0.05). Conclusion: As phloroglucinol has better curative effect and fewer side effects in the treatment of acute biliary colic, it is worth of promotion and utilization in the clinical medical treatment.
2016 Vol. 46 (3): 220-222 [Abstract] ( 682 ) HTML (1 KB)  PDF (1294 KB)  ( 1257 )
223 Mikulicz’s disease: a clinicopathological analysis of  51 cases
ZHAI Xiaoli, LOU Shanxian, SHI Hongqi, HU Bin, ZHANG Yanfei.
Objective: To explore the pathological features, differential diagnosis and prognosis of Mikulicz’s disease, and provide methods of treatment and prevention for clinicians. Methods: The clinical and pathological features of Mikulicz disease were analyzed in 51 patients and the expression of LCA, CD 20, CD 3, CD 4, CD 45RA, CD 45RO, EMA, CEA, S-100, CK, lysozyme, EBV, IgG4, HBsAg and HBcAg in Mikulicz disease were detected immunohistochemically. Results: Microscopically, the prominent features of Mikulicz disease were acinar atrophy and lymphoid tissue hyperplasia. Immunohistochemical staining showed that the lymphoid tissue was composed of a mixed population of B and T lymphocytes and about 80% of patients were IgG4 positive. Conclusion: Mikulicz disease is an autoimmune disease and mostly occurred in postmenopausal women. Postoperative recurrence may happen. Immunosuppressive agents may be helpful in treatment.
2016 Vol. 46 (3): 223-225 [Abstract] ( 665 ) HTML (1 KB)  PDF (1459 KB)  ( 989 )
226 The characteristics of pathogens and ana1ysis of curative effects in 66 periana1 abscess of infants under 3 months 
ZHU Yimin, XU Fuying.
Objective: Pathogen identification, antibiotics susceptibi1ity test and curative effects ana1ysis were conducted in perianal abscess of infants under 3 months to guide clinical treatment for such cases. Methods: Bacterial culture results, antibiotics susceptibi1ity test and curative effects of incision and drainage of abscess were retrospectively analyzed in 66 cases of periana1 abscess of infants under 3 months. Results: Forty-eight cases were Klebsiella pneumonia, 7 cases Staphylococcus, 6 cases Escherichia coli and 5 cases of proteus in the pathogen culture results. Klebsiella pneumonia-the common pathogenic bacteria was susceptible to the majority of antibiotics, especially to imipenem, cefoperazone-sulbactam and amikacin with low drug resistance rate. However, high drug resistance rates were found in ampicillin and nitrofurantion. After incision and drinage of abscess 6.6% complication of anal fistula was happened in infants under 3 months and 60.3% in the control adult group. There was significant difference P<0.01. Conclusion: Klebsiella pneumonia is the most common pathogen in periana1 abscess of infants under 3 months and was commonly resistant to ampicillin and nitrofurantion. Since it recovered well, periana1 abscess of infants under 3 months should be done with simple surgery.
2016 Vol. 46 (3): 226-228 [Abstract] ( 670 ) HTML (1 KB)  PDF (1291 KB)  ( 976 )
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