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2015 Vol. 45, No. 9 Published: 2015-09-25   625 Molecular regulatory mechanism of two-component regulatory system SaeRS on important virulence genes in Staphylococcus aureus XU Yuanyuan1, DING Yu2, LIU Yunling3, LI Dan2, WANG Liangxing3, YU Fangyou2. Objective: To investigate the molecular regulatory mechanism of two-component regulatory system SaeRS on important virulence genes in Staphylococcus aureus. Methods: The 687-bp saeR gene was PCR amplified and cloned into pET28a, resulting in the plasmid pET28a-saeR, then tested by restriction enzyme analysis, PCR and gene sequencing. The recombinant protein His SaeR was induced by IPTG, purified with Ni-NTA agarose and  verified by Western blot. The expression levels of luk-PV, hla, coa, fnbB, sak, psmβ genes were detected by real-time PCR. The binding abilities between SaeR and the promoter regions of virulence genes were tested by electrophoretic mobility shift assay. Results: Restriction enzyme digestion, PCR amplification and gene sequencing showed that the recombinant plasmid pET28a-saeR was successfully constructed. The recombinant protein His SaeR was efficiently expressed in soluble in E.coli BL21 (DE3) induced by 0.4 mmol/L IPTG at 25 ℃ for 12 hours. Compared to wild type strain SA75, the 1evels of luk-PV, hla, coa, fnbB mRNA of SA75ΔsaeRS mutant strain decreased to 19.7%, 0.3%, 31.6% and 3.5% respectively whereas the 1evels of psmβ and sak mRNA had no difference between wild type and mutant strain. The electrophoretic mobility shift assay showed that phosphorylated purified His SaeR could bind to the promoter regions of luk-PV, hla, coa, fnbB and P1 promoter. Conclusion: Two-component regulatory system SaeRS directly up-regulates the luk-PV, hla, coa, fnbB genes which is realized via response regulator protein SaeR combined with their promoter regions. 2015 Vol. 45 (9): 625- [Abstract] ( 731 ) HTML (1 KB)  PDF (1863 KB)  ( 1341 ) 631 Inhibition of Naringenin on mucous hypersecretion of A549 cell induced by A2 strain of respiratory syncytial virus MENG Shanshan1,2, LV Fangfang1, HU Xiaoguang1, ZHANG Hailin1, LI Changchong1 Objective: To investigate the expression levels of MUC5AC protein and mRNAin mucous hypersecretion model induced by respiratory syncytial virus (RSV), and explore the effect of Naringenin (Nar) on mucous hypersecretion induced by RSV. Methods: Human lung adenocarcinoma epithelial cells (A549) were subcultured, infected with RSV and treated with Nar 30-100 μmol/L. After it was treated for 24 hrs, the expression of MUC5AC at mRNA and protein level in the groups were determined by real-time quantitative PCR and enzyme-linked immunosorbent assay (ELISA). The morphological changes of MUC5AC protein were observed with immunofluorescence technology. Results: The expressions of MUC5AC protein and mRNA in all RSV infected groups were significantly higher than that in group C in a dose-dependent manners (all P<0.05). Nar of 30 and 100 μmol/Lsignificantly and dose-dependently decreased RSV-induced secretion of MUC5AC protein in cell supernatant and expression of MUC5AC mRNA (P<0.05), but still higher than that in group C (P<0.05). We also observed that RSV increased MUC5AC protein expression in the cytoplasm of A549 by immunofluorescence technology, and the protein expression decreased after pretreatment with Nar. Conclusion: Naringenin can inhibite the RSV-induced mucous hypersecretion of A549 cell. 2015 Vol. 45 (9): 631- [Abstract] ( 592 ) HTML (1 KB)  PDF (1732 KB)  ( 967 ) 636 Morphological study on upper airway structure in snoring children with multislice spiral CT CAI Xiaohong1, MEI Hongfang1, CAO Hongchao1, LIANG Dongshi2, YU Chenyi1, XU Chongyong3, NI Liyan4. Objective: To explore the structural features of upper airway morphological in snoring children with multislice spiral CT scan and post-processing techniques and to reveal the relationship between structural abnormalities and the severity of snoring. Methods: Children with snoring were enrolled and divided into primary snorer (PS) group (n=45) and obstructive sleep apnea hypopnea syndrome (OSAHS) group (n=73) based on polysomnography (PSG). Another 40 cases were enrolled as the control group (n=40). Multislice spiral CT scan covering upper airway was performed and measurements of soft tissue, bone structure and spatial structure of the upper airway were obtained from a 3-dimensional segmentation using image analysis workstation of phillips Corp. Results: The area, length, thickness and volume of adenoid, the area and the volume of the soft palate, the cross-sectional area of the tonsils in snoring children groups were significantly higher than those in the control group (P<0.05). The cross-sectional area of the the velopharyngeal, the anterroposterior diameter of the velopharyngeal, the nasopharynx volumes and the velopharyngeal volumes, the paranasal sinus volumes in snoring children groups were significantly reduced than that in the control group (P<0.05). The angle (α) was smaller and adenoid-nasopharynx ratio increased in snoring children groups than those in the control group (P<0.01). Conclusion: The snoring children have airway obstruction of various degrees at the upper airway because of the adenoid and tonsillar hypertrophy and the enlarged soft palate. Sinusitis is often complicatd and lower hyoid bone position moves down leading to increased upper airway resistance. 2015 Vol. 45 (9): 636- [Abstract] ( 694 ) HTML (1 KB)  PDF (2460 KB)  ( 979 ) 641 Correlative analysis between executive function of attention-deficit hyperactivity disorder in Han nationality people and the activity of monoamine oxidase A gene WANG Weiqian, DU Songmei, YANG Chuang, CHEN Hong, ZHAO Yongzhong, LIANG Yan, LIN Haixi, ZHANG Xiaoyan, WANG Changlv, WANG Meihao, HE Jincai. Objective: To explore the relationship between polymorphism of MAOA gene activity and the executive function of attention-deficit hyperactivity disorder (ADHD). Methods: Choose 37 patients who was diagnosed as ADHD and 30 normal volunteers as controls. The executive function was evaluated using wisconsin card sorting test (WCST) and Stroop test. Taking peripheral blood and restriction fragment length polymorphism (RFLP) techniques were performed to detect MAOA 30 bp tandem repeats multiple of variable number, according to this repeats multiple, the case and control groups were divided into high and low activity group, respectively. Results: Group factor had significant effects on conceptual level, the number of completely classifications, the number of wrong answers, the number of continually mistakes, the number of continually answers in WCST; Group factor also had statistical significant differences on the complete time between A and C card in Stroop test (P<0.001), the time of interference effects also had statistically significant (P<0.05); MAOA gene activity factor had significant influence on conceptual level, the number of continually mistakes and the number of wrong answers had statistically significant on the difference of complete time in STROOP A card and C card (P<0.05), the time of interference effects also had statistically significant (P<0.05). There was no statistically significant in allele distribution of MAOA gene between the ADHD cases group and the control group (P>0.05). Conclusion: Our study find no relationship between MAOA-uVNTR allele distribution and ADHD, However, there maybe have relationship between MAOA polymorphism and part of the executive function. 2015 Vol. 45 (9): 641- [Abstract] ( 623 ) HTML (1 KB)  PDF (1333 KB)  ( 1258 ) 646 Clinicopathological characteristics of KRAS and BRAF mutation in 138 patients with rectosigmoid cancer YANG Fan1, CAI Yefeng1, JIN Langping1, ZHANG Xiaohua2, QU Jinmiao2. Objective: To identify the mutation rate and mutation feature of KRAS and BRAF gene in patients with  rectosigmoid carcinoma, to analyse the association between KRAS/BRAF mutations and patients’ clinicopathological characteristics, and discuss the clinical significance of KRAS/BRAF mutations. Methods: 138 patients with rectosigmoid carcinoma were collected. DNA sequencing was used to detect mutations in KRAS (codons12, 13 of exon2 and codons 61 of exon3) and BRAF (exon15). Statistically analyzing the relationships between gene mutations and clinicopathological characteristic. Results: In 138 cases, the mutation rate of KRAS and BRAF was 34.05% (47/138) and 5.07% (7/138). 9 mutation types were found in KRAS, including a new mutation type. BRAF mutation was all belong to V600E mutation. In patients with rectosigmoid carcinoma, KRAS mutation rate was higher in male (P=0.041), distant metastasis (P=0.002) and high clinical stages (P=0.047). BRAF mutation rate was higher in multiple (P=0.003) and poorly differentiate (P=0.001) carcinoma. No significant associations were observed between KRAS/BRAF mutations and age, tumor size, pathomorphism, pathological type, depth of invasion, lymph node metastasis, vessel infiltration and nervous infiltration (P>0.05). Conclusion: Rectosigmoid carcinoma is unique. KRAS mutation rate is higher in male, distant metastasis and high clinical stages carcinoma. BRAF mutation rate is higher in multiple and poorly differentiate carcinoma.KRAS and BRAF mutations are variant in different location of colorectal cancer. 2015 Vol. 45 (9): 646- [Abstract] ( 728 ) HTML (1 KB)  PDF (2599 KB)  ( 1005 ) 652 The correlative analysis of eNOS rs1799983 single-nucleotide polymorphisms and cerebral infarction MAO Xinlei, HAN Liya, HUANG Xiangdong, GUAN Chaohong Objective: To investigate the relationship of eNOS rs1799983 single-nucleotide polymorphisms (SNP) and cerebral infarction. Methods: Data was prospectively collected from consecutive ischemic stroke inpatients in neurology department of our hospital from may 2014 to september 2014. Eighty-three large-artery atherosclerosis (LAA) patients and small-artery occlusion (SAO) patients in 100 patients were enrolled, according to the TOAST criteria. The control group was 91 subjects from the Medical Examination Center at the same period. Taqman probe technique was used for the detection of eNOS rs1799983 SNP. The clinical characteristics and eNOS rs1799983 SNP were compared between three groups. Results: As compared with the controls, there were statistically significant differences in percentage of HBP, smoking, drinking, high-density lipoprotein and cholesterol concentrations in LAA and SAO groups (P<0.01). The GG genotype was main genotype in three groups. Only 1 case TT genotype was observed in LAA group. The frequency of allele and genotype was not significantly difference in these three groups. Conclusion: It is suggested that there are similar risk factors in LAA and SAO patients. The eNOS rs1799983 SNP maybe unrelated to cerebral infarction. 2015 Vol. 45 (9): 652- [Abstract] ( 693 ) HTML (1 KB)  PDF (1306 KB)  ( 848 ) 656 Biocompatibility experiment of small intestinal submucosa with olfactory ensheathing cells LI Yu’an1, LI Jia2, XU Huazi1, LIN Damu1, WU Zhipeng1. Objective: To explore the biocompatibility of small intestinal submucosa as a biological scaffold on the proliferation of olfactory ensheathing cells. Methods: The porcine small intestinal submucosa (SIS) was made according to Abraham’s methods. seeding the OECs on the porcine SIS for experimental group (SIS+OECs), OECs cultivating alone as control group. Then OECs’ growth, adhesion and proliferation of OECs on SIS was examined by HE stained and scanning electron microscope at different time during the cultivation, and the proliferation of OECs was examined with CCK8 as well. Results: OECs could attach to SIS within 24 hours after seeded on the SIS. After being cultured 3 to 5 days, OECs were intensive on the edge of SIS, well adhered to it and the cells were most fusiform and tripolar in shape. The combination of SIS and OECs was very excellently examinated by scanning electron microscope and Haematoxylin and eosin stained. And CCK8 measurement also revealed that, the quantity of OECs reached a stable level at 7 to 9 day and this level would not decreased until 11 d. Compared with these in contrast group, the proliferation of OECs on SIS had obvious difference during the former 5 days (P<0.05) but there was no significant difference after 7 day (P>0.05). Conclusion: Porcine SIS has good biocompatibility with rat OECs, although there is no evidence showing SIS promoting OECs’ proliferation. SIS can expectedly be used in repair of spinal cord injury as a scaffold where OECs may grow and adhere. 2015 Vol. 45 (9): 656- [Abstract] ( 588 ) HTML (1 KB)  PDF (1406 KB)  ( 733 ) 660 Preparation of Synphilin-1 transgenic mouse model and study on the expression of Parkinson’s disease-related protein JIN Hongguo, TANG Weiguo, JIN Meiyu, HE Songbin, ZHOU Min, Kou Xuelian Objective: To explore the pathogenesis correlafion of Synphilin-1 with Parkinson’s disease. Methods: Generated transgenic mice expressing human Synphilin-1 under the prion protein prom-oter. The genotype of offspring mice was determined by a PCR assay of DNA obtained from tail biopsies.The human Synphilin-1 gene was injected into the fertilized ovum using prion promoter vector to generate the transgenic mice, then PD-related protein immunoblot analysis, the brain tissue slice analysis with immunohistochemistry, double labeling immunofluorescence staining and the phenotypic and athletic analysis of transgenic mice were performed. Results: Synphilin-1 was widely expressed in neurons in the brain, including the substantianigra, but massive loss of dopamine neurons was not observed. In the transgenic mouse brain, Synphilin-1 protein was polyubiquitinated and partially insoluble. Although modified-SHIRPA revealed no significant difference in behavior and morphology, the reduced rotarod performance and step length were observed in transgenic mice as compared with non-transgenic littermates. Conclusion: Synphilin-1 may be involved in motor function, and its accumulation in the central nervous system can cause motor impairments. Synphilin-1 may be involved in regulation of motor function, but had no significant effect of the expression of Parkinson’s disease related proteins nor dopamine neurons in the brain tissue. 2015 Vol. 45 (9): 660- [Abstract] ( 628 ) HTML (1 KB)  PDF (2937 KB)  ( 1154 ) 665 Analysis on the related factors of the negative pathology results of cervical loop electrosurgical excision procedure in patients with high-grade cervical squamous intraepithelial lesions LV Jiamin1,2, ZHU Objective: To investigate the related factors of the negative pathological results of loop electrosurgical excision procedure (LEEP) in patients with high-grade cervical squamous intraepithelial lesions (CIN2-3). Methods: Totally 263 patients diagnosed as CIN2-3 by colposcopy-assisted biopsy and treated with LEEP in Jiaxing Maternity and Child Care Hospital from March 2011 to December 2012 were selected, and the relation of the negative pathological results of LEEP with age, cervical cytology, HPV load and pathological grading was analyzed. Results: Univariate analysis showed that low HPV load (<400 RLU/CO), low-grade of TCT, biopsy-confirmed CIN2 were associated with the prediction of the negative pathology of LEEP specimens (P=0.008, P=0.012, P=0.02), however, age was not related to the negative result of LEEP (P=0.172). Multivariate logistic analysis showed that low HPV load was the independent factor of the negative pathological results of LEEP (P= 0.019, OR=3.752, 95%CI 1.247～11.293). The area under ROC curve was 0.727 for three factors (HPV load, TCT and biopsy pathology diagnosis) predicting negative pathological result of LEEP. Conclusion: Low HPV load is related to the negative pathological results of LEEP. Combining low HPV load with low-grade of TCT and biopsy-confirmed CIN2 has a high predictive effect on negative pathological results of LEEP. 2015 Vol. 45 (9): 665- [Abstract] ( 603 ) HTML (1 KB)  PDF (1348 KB)  ( 984 ) 671 The influence of different loading doses of Urapidil injection on blood pressure and the door-to-needle time in acute ischemic stroke TAO Xiaoxiao, WANG Peng, JIN Yunlong, XIN Huaping, XIAO Yu, ZHENG Xian, HU Meiling, HUANG Yangping, YE Jiamei, PENG Shuangshuang, WANG Jun, WANG Zhimin Objective: To observe the effect of different doses of Urapidil injection before thrombolysis in acute ischemic stroke on the blood pressure or door-to-needle time (DNT). Methods: Thirty-six cases in our hospital who were treated with Urapidil injection before intravenous t-PA treatment were retrospectively analyzed and were divided into 3 groups A, B, C according to the loading dose. Group A was with a loading dose of 5 mg Urapidil injection, Group B was with a loading dose of 12.5 mg, and Group C was with a loading dose of 25 mg. At last, shrinkage pressure (SBP), diastolic blood pressure (DBP), DNT and 3-month MRS score among the three groups were compared. Results: Compared to group A in which SBP was (173.27±6.92) mmHg and DBP (81.73±7.71) mmHg, the blood pressure of group B, with SBP (165.80±7.76) mmHg and DBP (80.93±6.95) mmHg, and group C, with SBP (154.80±10.69) mmHg and DBP (73.60±5.13) mmHg, decreased significantly (P<0.001). Compared to group A with (89.00±25.76) min, the DNT of group B with (76.73±20.20) min and group C with (73.10±14.15) min the door-to-needle time (DNT) significantly reduced (P<0.001). In addition, the percentage of MRS scores (0-1 points scale) after three months in group B (60.0%) was the highest among the three groups (P<0.001). Conclusion: Urapidil injection with the first load of 12.5 mg can effectively decrease blood pressure to standard as soon as possible with a better outcome. 2015 Vol. 45 (9): 671- [Abstract] ( 685 ) HTML (1 KB)  PDF (1318 KB)  ( 998 ) 674 Efficacy of ziprasidone and olanzapine in treating patients with schizophrenia and their influences on blood glucose and glycosylated hemoglobin at early stage of course of treatment ZHOU Yong1,2, HUANG Hanjin2, TANG Jianliang3. Objective: To explore the efficacy of ziprasidone and olanzapine in treating patients with schizophrenia and their influences on blood glucose and glycosylated hemoglobin at early stage of course of treatment. Methods: 197 cases of schizophrenia patients were randomly divided into experimantal group (99 cases administered only with ziprasidone) and control group (98 cases administered with ziprasidone and olanzapin). All the patients were selected from 7 different psychiatric hospital who were diagnosed from 2013.1-2014.1. Both groups were administered with olanzapin and olanzapine at the first 5 weeks. Then olanzapine was abandoned gradually in experimental group, while the control group went on its original treatment both groups were treated for 12 weeks. Scores of PANSS, level of blood glucose and glycosylated hemoglobin were compared between the two groups before treatment and the 1st, 5st, 7st, 9st, 12st weekend after treatment. Results: Both groups showed significant decrease after the treatment (P<0.05), but there’s no statistical difference at every time point (P>0.05). The level of limosis blood glucose in control group was higher than that in experimental group at the 9th and 12th weekend (P<0.05). There’s no statistical difference at the level of glycosylated hemoglobin in both groups by every time point (P>0.05). Conclusion: The efficacy of single ziprasidone and combination treatment is similar, but single ziprasidone at later stage shows more benefit for blood glucose control and meanwhile shows relatively reliable efficacy. 2015 Vol. 45 (9): 674- [Abstract] ( 884 ) HTML (1 KB)  PDF (2060 KB)  ( 1370 ) 679 Correlative analysis between the quality of life and social support in patients with recurrent ovarian cancer#br# SHAO Zhuyan, WEN Qiang, LI Dan, ZHANG Ping. Objective: To evaluate the quality of life (QOL) and the social support in patients with recurrent ovarian cancer, and to analyze the relationship between them. Methods: 122 patients with recurrent ovarian cancer were interviewed with two questionnaires: Quality of Life Questionnaire C30 (QLQ-C30) and Perceived Social Support Scale (PSSS). Results: QOL declined in recurrent ovarian cancer, with poor global health score (56.48±22.02). Among positive ratings in five functional domains, social functioning score (64.48±25.70) was the lowest. And among reverse ratings, financial difficulties (49.45±34.61) were the most main factors reducing QOL. The social support in patients with recurrent ovarian cancer was good. The mean score of total social support was 5.64±0.94, but the mean score of family support inside (6.14±0.84) was higher than that of family support outside (5.39±1.16). The difference was statistically significant (P<0.01). It showed a negative correlation between social support and financial difficulties. Family support inside had positive correlation with score of cognitive functioning and social functioning, and negative correlation with score of fatigue and pain (P<0.05). Conclusion: Patients with recurrent ovarian cancer have poor QOL. The social support of them closely related with QOL, and we should use the social support sufficiently to improve QOL of patients. 2015 Vol. 45 (9): 679- [Abstract] ( 694 ) HTML (1 KB)  PDF (1314 KB)  ( 1066 ) 683 Imaging diagnosis of struma ovarii ZHANG Yu, MEI Haibing. Objective: To analyze the MR and ultrasound (US) features of struma ovarii (SO). Methods: MR and US images of 8 patients with pathologically proved SO were retrospectively analyzed. Imaging features were compared with pathological results. Results: All tumors were unilateral, well-defined, mixed solid and cystic. The maximum sizes of the tumors ranged from 5.1 cm to 19.3 cm. MR appearances: The solid components were intermediate intensities on T2-weighted images and high intensities on diffusion-weighted images. The signal intensities of the cystic components were various. The areas of prominent low intensity on T2-weighted images were recognized in all cases, which were low intensities on diffusion-weighted images. Cyst walls, septa and solid components were well enhanced. Ultrasonographic appearances: The solid portion of tumors showed parenchymal echogenicity with “struma pearls” in 7 cases. Conclusion: Struma ovarii appeared as a smooth marginated mass with the areas of prominent low intensity on T2-weighted images on MR, and “struma pearls” on US images. The MR and US characteristic appearances of SO may be of great value for the diagnosis. 2015 Vol. 45 (9): 683- [Abstract] ( 728 ) HTML (1 KB)  PDF (1475 KB)  ( 1126 ) 687 Effect of dexmedetomidine on hepatic function in patients undergone laparoscopic cholecytectomy with CO2 pneumoperitoneum CHEN Li1,2, LU Bin2, WU Ansheng2, LI Jun1. Objective: To evaluate the effect of dexmedetomidine on hepatic function in patients undergone laparoscopic cholecystectomy with CO2 pneumoperitoneum. Methods: Sixty patients aged 18–60 years, whose pneumoperitoneum duration varied from 0.5 to 1 h and matched ASA I-II, were randomly divided into two groups: control group (group C, n=30) and dexmedetomidine group (group D, n=30). In group D, dexmedetomidine was infused intravenously at a rate of 1 μg•kg-1 for 10 min, followed by 0.3 μg•kg-1•h-1 until the end of the operation, while in group C equal volume of 0.9% sodium chloride solution was infused after trachea intubation. Peripheral vein blood samples were collected immediately before anesthesia (T0, baseline), 1 h (T1) and 24 h (T2) after pneumoperitoneum released for measurement of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), plasma IL-6, TNF-α, malondialdehyde (MDA) and superoxide dismutase (SOD) levels. Results: Compared with T0 point, the serum ALT, AST activities were significantly increased at T2 in both groups. Compared with group C, the serum ALT, AST, plasma TNF-α and IL-6 were significantly descreased at T2 and the serum MDA was significantly descreased at T1, T2 and the levels of plasma SOD at T1 was significantly increased in group D (P<0.05). Conclusion: Hepatic injury in patients undergone laparoscopic cholecystectomy with CO2 pneumoperitoneum can be attenuated by dexmedetomidine via inhibiting inflammatory responses and lipid peroxidation. 2015 Vol. 45 (9): 687- [Abstract] ( 600 ) HTML (1 KB)  PDF (1298 KB)  ( 916 ) 690 Application of ultraosonography combined with fine-needle aspiration cytopathology on evaluating thyroid nodules GONG Wei1, FANG Weijian1, CHEN Guorong2, FENG Yaqin1 Objective: To explore the application of ultrasonography combined with fine-needle aspiration (FNA) cytopathology on evaluating thyroid nodules. Methods: A total of 2 491 cases of thyroid surgery, of whom 249 cases were malignant from 2010 February to 2014 July were analyzed retrospectively, including the characteristics of ultrasonography and FNA cytopatholgoy. The former consisted of thyroid’s volume, number, size, hypoechogenecity, blur margin, microcalcification and taller-than-wide shape of thyroid nodule, the later included nuclear grooves, pseudoinclusions, ground glass and papillary architecture. Results: The single marker in thyroid ultrasonography related with malignancy was nodular microcalcification, single nodule larger than 2 cm and taller-than-wide nodule (all P<0.05), but all of which couldn’t tell the nature of thyroid nodules. The diagnostic sensitivity and specificity of FNA cytopathology on thyroid cancer was 88% and 77%, and the combination of ultrasonography and FNA cytopathology was 71% and 96%. Conclusion: In this regard of distinguishing the difference of benign and malignant nodule of thyroid, ultrasonography combined with FNA cytopathology is the first line and cost-effective evaluation method. 2015 Vol. 45 (9): 690- [Abstract] ( 620 ) HTML (1 KB)  PDF (1319 KB)  ( 899 ) 694 The distinguishing meaning of CT perfusion on the blood perfusion of the area of cranio-cerebral trauma focus DAI Junxia, SUN Jun, CHEN Maohua Objective: To explore the distinguishing meaning of CT perfusion (CTP) on the blood perfusion condition of the area of cranio-cerebral trauma focus. Methods: CT scanning was used in the patients with different cranio-cerebral trauma. Furthermore, CTP was used to examine those lesions with low-density shadow surrounding, while an analysis of the parameters of mean transit time (MTT), regional cerebral blood flow (rCBF) and regional cerebral blood volume (rCBV), and a comparison of patient prognoses were also conducted. Results: CT scan showed low-density focus of the brain parenchyma around the site of the trauma in 79 cases; CT perfusion parameters showed hypo-perfusion of the brain tissue around the trauma in 42 cases (54.4%), high perfusion in 35 cases (44.3%), while there were no obvious brain tissue perfusion abnormalities in 2 cases. Prognosis of low perfusion was poorer. Conclusion: CT examination should apply further CTP in the case of those patients who have been found to display low-density shadow around their cranio-cerebral trauma, in order to help clinicians to better understand the local haemodynamic state of the brain tissue. This has great significance on the diagnosis of focus microcirculation disturbance of cranio-cerebral trauma. 2015 Vol. 45 (9): 694- [Abstract] ( 694 ) HTML (1 KB)  PDF (1582 KB)  ( 981 ) 697 The diagnostic value of female paraurethral cyst by transperineal and transvaginal sonography HUANG Junying1, ZONG Fang1, HONG Jie1, HUANG Pintong2. Objective: To explore the diagnostic value of female paraurethral cyst by transperineal sonography (TPS) and transvaginal sonography (TVS). Methods: Twenty-one patients of female paraurethral cyst were examined with transabdominal sonography (TAS) and TPS or TVS, images were analyzed by contrast, and compared with surgical pathological findings. Results: All lesions were shown more clearly by TPS and TVS than TAS, especially the former could clearly show the relationship between the lesions and the wall of urethra and vaginal. TPS and TVS could also display internal echoes and the distribution of color doppler flow imaging (CDFI) of the lesions more clearly, TPS and TVS did not influence by poor filling of the bladder, obesity and other factors. Conclusion: TPS and TVS have higher accuracy than TAS in the diagnosis of female paraurethral cysts. 2015 Vol. 45 (9): 697- [Abstract] ( 594 ) HTML (1 KB)  PDF (2440 KB)  ( 1173 )

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