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2015 Vol. 45, No. 8 Published: 2015-08-25   547 Experimental study on the development and targeting effect of nanoparticles of double-antibody complex and adriamycin loaded ZHENG Yijing, ZHANG Xiaolei, LIN Yan,WU Yaosen, ZHANG Qiong Objective: To synthesize double antibody complex-glycol chitosan doxorubicin nanoparticles (Abc-GC-Dox) and study its targeting effect of osteosarcoma in mice. Methods: Abc-GC-Dox and GC-Dox were prepared by emulsion polymerization method, in vitro immunofluorescence was used to study the intake test of HER2+carried MG-63 cells in Abc-GC-Dox and GC-Dox. Fourteen mice with osteosarcoma were randomly divided into 2 groups. After corresponding treatment, different drugs (Abc-GC-Dox and GC-Dox) were given via tailvein, and the targeting effect was observed. Results: Stable Abc-GC-Dox and the quantity of drug • loading and entrapment were gotten. Compare to GC-Dox, there was a better drug concentration in osteosarcoma in mice, which showed a well targeting effect in vivo. Conclusion: Abc-GC-Dox is successfully developed and the nanoparticles demonstrated targeting aggregating distribution. In future, Abc-GC-Dox can be a potential drug for the clinical treatment of tumors. 2015 Vol. 45 (8): 547- [Abstract] ( 713 ) HTML (0 KB)  PDF (1821 KB)  ( 1132 ) 552 Antifeeding and toxic activity of ethyl acetate extract from barks of Melia azedarach L against Spodoptera litura larvae  GE Junjun1, CHEN Pian1, HUANG Yilu1, FENG Bo2, YE Xiaoxia1. Objective: In order to find new compounds with strong antifeeding activities, the crude extract and fractions from barks of Melia azedarach L. were selected for their antifeeding and toxic activity against Spodoptera litura larvae. Methods: The 95% ethanol extract of the barks of Melia azedarach L. was concentrated, and the aqueous solution was partitioned with ethyl acetate. The ethyl acetate extract was subjected to silica gel CC eluted with ether-ethyl acetate to give 15 fractions (K1～K15). The fractions were investigated against Spodoptera litura larvae by leaf disc method to test the leaf area and the mortality. Results: Ethyl acetate extract from barks of Melia showed significant antifeeding activity against Spodoptera litura larvae, which was improved with increased dosages. Fractions 5, 6, 10 displayed strong toxic effects, and Fraction 11 also indicated toxic effects. Fractions 5, 6 revealed strong antifeeding activity even at the low concentration. Conclusion: Fractions 5, 6 demonstrate obvious antifeeding and toxic activity against Spodoptera litura larvae than Fractions 10, 11. The fractions which reflect stronger antifeeding activity will be isolated and purifies in hoping of achieving the new active compounds. 2015 Vol. 45 (8): 552- [Abstract] ( 1328 ) HTML (0 KB)  PDF (1326 KB)  ( 1228 ) 556 Levosimendan improves survival rate in lipid-epinephrine based resuscitation in a rat model of bupivacaine-induced cardiac arrest NAN Fubei1, WU Bingjing1, DONG Jiaojiao1, XIA Yun2, Thomas J.Papadimos2, XU Xuzhong1. Objective: To observe the effect of levosimendan on resuscitation using lipid combined with epinephrine in a rat model of bupivacaine-induced cardiac arrest. Methods: Twenty-four adult SD male rats were randomly divided into two groups: lipid in combination with both epinephrine and levosimendan (LLE, n=12)or lipid in combination with epinephrine (LE, n=12). Bupivacaine was injected intravenously to establish a rat model of cardiac arrest. Then regular external chest compression was started, and lipid emulsion combined with a small dose of epinephrine resuscitation was used for resuscitation. Additionally, the experimental group was treated with levosimendan, when the control group was given 0.9% sodium chloride. As our primary outcome was the survival rate at 45 mins, cases of successful resuscitation and rats survived to 45 mins were recorded. Besides, the time to the first heart beat and restoration of spontaneous circulation (ROSC), the amount of epinephrine, the wet to dry ratio of the lung, and the arterial blood gas data at the end of the balance period and at 45 min were also calculated. Results: The survival rate at 45 min in the LLE group was higher than that in the LE group (P<0.05). The time to the first heart beat and ROSC in the LLE group was shorter than that in the LE group (P<0.05). The amount of epinephine in LLE group was less than that in the LE group (P<0.05). The arterial blood gas data such as PH, BE, PaCO2 and PaO2 in the LLE group were all better than those in the LE group at 45 mins (P<0.05). Conclusion: In the rat model of lipid-epinephrine based resuscitation from bupivacaine-induced cardiac arrest, the combination with levosimendan improves the survival rate and accelerates the recovery. 2015 Vol. 45 (8): 556- [Abstract] ( 599 ) HTML (0 KB)  PDF (1429 KB)  ( 848 ) 560 Neruoprotective effect of bFGF on ischemic-reperfusion injury in SD rats via intranasal administration JIANG Yina1, TIAN Furong1, LIN Qian1, YAO Rui2, ZHAO Yingzheng1. Objective: To investigate the neuroprotective effects of bFGF (basic fibroblast growth factor) via intranasal administration for ischemic neurons of brain. Methods: The transient focal cerebral ischemia-reperfusion model of adult male SD rats was established with a method of line-plugging to the external carotid artery. Twenty-four hours after the reperfusion, behavioral test (neurologic deficit scoring) was carried out to evaluate the neurological deficiency. The neuroprotective effects of bFGF via intranasal administration to the model rats were evaluated with neurologic deficit score, infarct volume, histology and immunohistochemistry staining. Results: The transient focal cerebral ischemia-reperfusion model were established successfully. Comparing with the ischemia-reperfusion group, the level of infarction volume was significantly decreased (P<0.05), the number of nerve cells were increased and the expression of p-Akt positive cells were significantly up-regulated via intranasal administration of bFGF group. Conclusion: By the new pathway of nasal administration, bFGF can decrease the infarction volume and protect neurons from cerebral ischemic injury. 2015 Vol. 45 (8): 560- [Abstract] ( 639 ) HTML (0 KB)  PDF (1804 KB)  ( 1088 ) 566 Comparison of biological properties of enteric neural stem cells in two ways of culturing in vitro LIU Miaoqing, WANG Yongbiao, HUANG Xiaozhong, ZHU Libin, CHEN Xiaoming, LI Zhongrong. Objective: To find a suitable proliferation way of culturing enteric neural stem cells in vitro, and to compare the biological characteristics of stem cells which were cultured in dishes coated and uncoated with fibronectin (FN). Methods: Enteric neural stem cells were isloated from gut of 15 days rat embryos, the neurospheres of fifth days were extracted from two groups. Growth status, proliferation and differentiation capacity of cells were compared through morphological observation, immunofluorescence cytochemistry and flow cytometer analysis between the two groups. Results: Compared with the FN coated group, the neurospheres of fifth days were the state of semi-adherent growth, the neurospheral morphology was regular, and were beginning to get dark in the center of them, and the spheres did not form a large amount of nerve fiber. Under fluorescence microscope, the typical neurospheres which were shown positive to BrdU in two groups were found. Radial nerve fiber of the neurospheres were positive to Tuj-1 (neuronal axons). After culturing for 5 days, compared with treatment with FN group (12.67±2.16)%, flow cytometer analysis showed positive to Nestin of the FN uncoated group had inacresed significantly (20.69±2.15)% (P<0.05); BrdU which mark proliferative cell increased significantly (35.82±2.11)% vs FN group (12.69±1.13)% (P<0.05); The percentage of Tuj-1 positive to neuron decreased significantly (10.67±1.14)% vs FN group (21.77±2.53)% (P<0.05). Conclusion: The way of uncoating culture reduces the time of forming neurospheres which were regular shape; maintains stem cell self-proliferation, reduce enteric neural stem cell differentiation and is suitable for stem cell self-proliferation in vitro. 2015 Vol. 45 (8): 566- [Abstract] ( 578 ) HTML (0 KB)  PDF (1494 KB)  ( 838 ) 570 Impact of calcified plaque on the diagnostic accuracy in detecting coronary stenosis using 320-slice MDCT XIE Furong, XU Huazhi, JIANG Yiyi, CAO Guoquan, WU Enfu Objective: To investigate the diagnostic accuracy of 320-slice dynamic volume coronary CT angiography (320-CCTA) in detecting coronary stenosis and to analyze the impact of coronary calcium in different calcification burden groups. Methods: The retrospective analysis enrolled 161 cases undergoing 320-CCTA and thereafter invasive coronary angiography (CAG) within 4 weeks for suspected coronary artery disease. Compared to CAG, the pretest probability of coronary stenosis was analyzed; the receiver operation characteristic (ROC) curve and area under curve of ROC (AUC) were used for statistical analyses. Results: Of 161 patients, 121 cases (75.2%) with optimal imaging quality (scale 4), 40 patients (24.8%) with adequate quality (scale 3). No any stair-step artifact or motion artifact in all cases. A total of 182 (7.9%) segments had some degree of calcification, of all segments, 136 (5.9%) had lightly calcification, 39 (1.7%) had moderate calcification, 7 (0.3%) had severely calcification. The sensitivity and specificity of 320-CCTA for detecting coronary stenosis were at least 50%: noncalcified plaque group (NCP) 98.3%, 81.3%, mixture plaque group (MP) 100%, 86.2%, lightly calcified plaque group (LCP) 78.6%, 99.2%, moderately calcified plaque group (MCP) 90%, 86.2%, severely calcified plaque group (SCP) 100%, 66.7%, the AUC was 0.90, 0.93, 0.89, 0.88, 0.83, respectively. Conclusion: Generally 320-CCTA provides high diagnostic accuracy; but the accuracy will be decreased due to severely calcification. 2015 Vol. 45 (8): 570- [Abstract] ( 642 ) HTML (0 KB)  PDF (1473 KB)  ( 822 ) 574 The renal pathologic types of adult primary glomerular diseases in Wenzhou: an analysis of evolution in 20 years YE Teng, LV Yinqiu, CHEN Bo, LI Fanfan, CHEN Xiaoqian, HUANG Zhaoxing. Objective: To investigate the renal pathologic types of adults’ primary glomerular diseases (PGD) including primary nephrotic syndrome (PNS), and the changing spectrum in 20 years in Wenzhou. Methods: Totally 3 392 adult (≥18 years of age) cases of biopsy-proven primary glomerular diseases diagnosed in the period of 2000 to 2013 in the Department of Nephrology of our hospital were retrospectively investigated, the renal pathologic types of all cases were analyzed and another 553 PGD cases diagnosed in the period of 1993 to 1999 in the same department were analyzed as control. Results: ①All of the 3 392 cases of PGD patients, the median age was 37 (29, 48)y, male:female ratio=1:1. The leading PGD was IgA nephropathy (IgAN) (accounts for 59.5%), followed by membranous nephropathy (MN) (accounts for 16.5%) and minimal change disease (MCD) (accounts for 12.4%). MN was the most frequent finding in early stage of the elderly and elderly patients (accounts for 46.6% totally), whereas in young and middle-aged adults, IgAN was the commonest (accounts for 47.6% and 41.2%, respectively). ②Compared with those diagnosed in the period of 1993-1999, the incidence rate of MN was two-fold increased in 2000-2013 (accounts for 16.5% and 7.2%, respectively). Among 1 067 cases of PNS diagnosed in 2000-2013, the dominant renal histopathological finding was MN (accounts for 38.7%), followed by MCD (accounts for 38.3%), IgAN (accounts for 13.0%) and focal segmental glomerular sclerosis (FSGS, accounts for 6.2%). Conclusion: IgAN remains the commonest renal pathologic type of PGD which is correspondence with our previous study. MN become predominant among PNS and increases significantly in recent years. Without the change of indications for renal biopsy, the evolution of renal pathologic types in PGD over the past 20 years is probably related to the actual change of the kidney diseases in Wenzhou. 2015 Vol. 45 (8): 574- [Abstract] ( 614 ) HTML (0 KB)  PDF (1341 KB)  ( 999 ) 579 The value of application of diurnal peak expiratory flow variability and asthma control test in monitoring patients with bronchial asthma JIN Lingling, LIN Hui, DAI Yuanrong. Objective: To explore the value of application of diurnal peak expiratory flow variability and asthma control test (ACT) in monitoring patients with bronchial asthma. Methods: Sixty-four asthmatic patients were collected from section for outpatients of the Second Affiliated Hospital of Wenzhou Medical University dating from July 2013 to December 2013. Their peak expiratory flow readings were determined with four times a day (6:00, 12:00, 18:00, 24:00). The diurnal peak expiratory flow variability and the average individually were calculated, at the same time the ACT and the first breath rate of expected percentage (FEV1%) were recorded. Results: Diurnal peak expiratory flow variability showed no relationship with FEV1% and ACT scores, but ACT had positive correlation with FEV1% (r=0.658, P<0.05), the linear regression relationship was Y=49.79+1.48X. The area under the curve in receiver operator characteristic curve of diurnal peak expiratory flow variaility was 0.976 (cutoff point 0.2, sensitivity 100% and specificity 85.4%). The area under the curve in receiver operator characteristic curve of ACT was 0.771 (cutoff point 20, sensitivity 65.2% and specificity 64.6%). The area under the curve in receiver operator characteristic curve of diurnal peak expiratory flow variability together with ACT was 0.767 (sensitivity 45%, specificity 100%). ACT of patients with poorly controlled showed no correlation with diurnal peak expiratory flow variability, but in patients with well controlled, the ACT scores and diurnal peak expiratory flow variability had negative correlation (r=-0.602, P<0.01). Conclusion: ACT score can be used to speculate asthma patients’ FEV1% lung function index. Diurnal peak expiratory flow variability and ACT can be used in monitoring asthmaseparatly,so can they used together. In patients with asthma better controlled, doctoers can separate peak expiratory flowvariation rate by ACT score. 2015 Vol. 45 (8): 579- [Abstract] ( 760 ) HTML (0 KB)  PDF (1534 KB)  ( 1010 ) 583 The diagnostic value of human papilloma virus E6/E7 mRNA detection in CINII+ HUANG Lingxiao1, PAN Qionghui1, ZHENG Jianqiong1, ZHU Xueyan1, ZHU Xueqiong2. Objective: To evaluate the application value of detecting human papilloma virus (HPV) E6/E7 mRNA as a diagnose test for CINII+. Methods: From January to October in 2014, a total of 426 gynecological outpatients, whose TCT≥ASCUS or/and high-risk HPV DNA positive, were found by cervical lesions screening in Wenzhou People’s Hospital. All those outpatients performed HPV E6/E7 mRNA detection and histopathological examination. According to different histological results, samples were divided into 5 groups, the correlation between the expression of HPV E6/E7 mRNA and the severity of cervical lesions were analyzed. The ROC curve was used to assess the predictive value of HPV E6/E7 mRNA for CINII+ of ASCUS. Results: The cervical cancer group had the highest level of HPV E6/E7 mRNA while the chronic cervical inflammation group had the lowest. Spearman rank analysis demonstrated that the severity of cervical lesions was proportional to the expression of HPV E6/E7 mRNA (r=0.549, P<0.001). For CINII+, there was no significant difference between HPV E6/E7 mRNA and high-risk HPV DNA in positive rate (x2=0.197, P>0.05). The ROC curve showed that HPV E6/E7 mRNA>147.965 were superior to any other standards to scan CINII+. The sensitivity and negative predictive value of HPV E6/E7 mRNA and HR-HPV DNA in predicting CINII+ of patients with ASCUS showed no statistically significant difference (P>0.05), while the specificity and positive predictive value showed statistically significant difference (P<0.05). Conclusion: The copy number of HPV E6/E7 mRNA transcripts is correlated with severe degree of epithelial cervical dysplasia. The specificity and positive predictive value of HPV E6/E7 mRNA detection are higher than those of HPV DNA for diagnosis of CINII+ in patients with ASCUS, therefore HPV E6/E7 mRNA detection may be a valuable tool for triage and patient follow-up. 2015 Vol. 45 (8): 583- [Abstract] ( 993 ) HTML (0 KB)  PDF (1438 KB)  ( 1507 ) 588 The study of bone conduction auditory brainstem responses in infants with normal hearing  CHEN Jun, WANG Jingbo, PENG Jianhua, FANG Xiaobi, JIN Lanlan, CHEN Xiaoyun. Objective: To study the characteristics of wave forms of the bone-conduction click-evoked auditory brainstem responses in 0～2 years old infants with normal hearing and to evaluate initially the value of its application in clinic. Methods: Air-and bone-conduction click-evoked ABRS in 24 cases of 0-2 years old infants (0-1 years, 1-2 years every 12 cases, 36 ears) with normal hearing were performed. The data were statistically analyzed with SPSS17.0. Results: The wave forms of bone-conduction ABR were almost the same as those of air-conduction at the thresholds or above. The detection rates of wave I, III and V were 29.2%, 46.7% and 100%, respectively at 35 dBnHL. The thresholds to bone-conducted ABR were (15.00±5.48) dBnHL, with the latencies of wave V were (8.32±0.54) ms. There were significant differences between ages for the thresholds and the wave V latencies (P<0.05), for aged 0-1 years infants, the thresholds to bone-conducted ABR were higher than that aged 1-2 years infants, and the wave V latencies were prolonged than aged 1-2 years groups. There were no significant differences between male and female for the thresholds and the wave V latencies. The bone-conducted wave V latency-intensity functions exhibited increased latencies as intensity decreased. Conclusion: The bone-conduction ABR is a valuable and effective method in children’s hearing test with special values in newborn hearing screening, more information on ascertaining the presence and magnitude of a conductive hearing loss can be obtained when both air and bone-conducted ABR wave V latency-intensity functions are administered. 2015 Vol. 45 (8): 588- [Abstract] ( 926 ) HTML (0 KB)  PDF (1548 KB)  ( 2553 ) 593 An improvement in primary culture of rat vascular smooth muscle cells and its identification MENG Liping1,2, JIANG Chengjian1, ZHAO Fei1,2, GUO Yan3, CHI Jufang1, GUO Hangyuan1,2. Objective: To explore a new effective way on primary culture of rat vascular smooth muscle cells (VSMCs), which could shorten the cultivate time and enhance the VSMCs’ purity. Methods: The primary culture of rat VSMCs was conducted by a new way reformed from the traditional modified tissue explant technique, in which the medial layer was isolated and 10 μg/L PDGF-B was added to the high glucose DMEM. The VSMCs were identified by cell morphology and immunocytochemistry. Results: About six day later, VSMCs could be found around some tissue blocks, and passage of the VSMCs can be done about 12 days later. Under the phase contrast microscope, the VSMCs looks like spindle and the specific “hill and valley” phenomenon could be seen. Immunocytochemistry showed that there was a large number of smooth muscle actin (SMA) and the VSMCs’ purity was much higher than the cells got by the old way. Conclusion: Adding PDGF-B could shorten the cultivate time of primary VSMCs and isolation of the medial layer could improve the VSMCs’purity. 2015 Vol. 45 (8): 593- [Abstract] ( 740 ) HTML (0 KB)  PDF (1499 KB)  ( 1087 ) 597 Clinical studies of pregnant outcome of early-onset intrahepatic cholestasis of pregnancy CHEN Cong1, CHEN Lingxiang1, WU Xueqing2. Objective: To analyze the clinical characteristics and perinatal outcome of early-onset intrahepatic cholestasis of pregnancy (ICP). Methods: A total of 226 ICP cases were collected in the Third Clinical College of Wenzhou Medical University between Jan 2010 and Dec 2013. According to the onset time of ICP, patients were divided into early-onset ICP group (onset time<28 gestational weeks) and late-onset ICP group (onset time≥28 gestational weeks), 200 normal pregnant women were randomly enrolled as controls. The levels of maternal serum total bile acid (TBA), total bilirubin (TBIL), alanine aminotransferase (ALT) and serum total bile acid (TBA) were evaluated. The perinatal outcome were assessed. Results: ①The maternal serum concentrations of TBA in early-onset ICP group were higher than that in late-onset ICP group before and after treatment, and the difference between the two groups was statistically significant (P<0.05). ②The early-onset ICP group had significant higher (P<0.05) incidence of admission to the neonatal unit compared to the late-onset ICP group and the control group. There was no significant difference (P<0.05) in incidence of premature delivery, fetal distress, fetal growth retardation, newborn asphyxia, cesarean section and average birth weight between the early-onset ICP group and the late-onset ICP group. ③The ICP group had significant higher (P<0.05) incidence of premature delivery, fetal distress, cesarean section and admission to the neonatal unit when compared to the control group. The average birth weight in the ICP group was lower than that in the control group. Conclusion: The ICP patients presents worse clinical manifestations than the controls. And ICP is much more likely to lead to fetal distress. The level of serum TBA is markedly elevated in the early-onset ICT patients. The incidence of admission to the neonatal unit is significantly higher compared to the controls. We can’t ignore the long-term management of patients with early-onset ICP. 2015 Vol. 45 (8): 597- [Abstract] ( 677 ) HTML (0 KB)  PDF (1313 KB)  ( 775 ) 601 Effects of different treatment opportunities of patients with gestational diabetes mellitus on maternal and neonatal outcomes ZHENG Zhi1, LV Jieqiang2. Objective: To explore the effects on maternal and infant outcomes of different treatment opportunities of patients with gestational diabetes mellitus and provide references for clinical treatments. Methods: From August 2013 to August 2014, 276 cases of patients with gestational diabetes mellitus in Wenzhou People’s Hospital were analyzed retrospectively. Patients diagnosed and treated before 32 pregnancy weeks were set as group A, while the rest patients confirmed and treated at 32 pregnancy weeks and later were enrolled into group B. The patients’ pregnancy outcomes were compared between two groups. Results: The occurrences of patients’ and perinatals’ complications in group A were much lower than group B (P<0.05); the neonatal body masses and gestational ages in group A were significantly better than those in group B, the differences are statistically significant (P<0.05). Conclusion: For patients with gestational diabetics mellitus, maternal and neonatal outcomes are related to the treatment opportunities, and detection before 32 pregnancy weeks and standardized treatment can effectively control and reduce the occurrences of adverse maternal and infant pregnancy outcomes. 2015 Vol. 45 (8): 601- [Abstract] ( 641 ) HTML (0 KB)  PDF (1339 KB)  ( 1114 ) 604 The clinical value of arginine stimulation test for testing the function of islet β-cell LIN Dini, GU Xuemei, SHEN Feixia, SHI Mengte, ZHU Qihan, CHEN Xiong. Objective: To observe and assess the value of arginine stimulation test for testing the function of islet β-cell of patients with T2DM. Methods: The function of islet β-cell of 20 hospitalized patients with type 2 diabetes was evaluated using arginine stimulation test and standard bread meal. Assess the safety and efficiency of arginine stimulation test and estimate its clinical value for patients with T2DM. Results: There were no significant differences of blood glucose between each time point of arginine stimulation test (P=0.918); And the plasma glucose levels of 60 min and 120 min after the standard bread meal test were significantly higher (P=0.000). Insulin area under the curve, mean value, peak value of arginine stimulation test were positive correlation with insulin area under the curve of the standard bread meal test. Conclusion: Arginine stimulation test showed a superiority to assess islet function of patient with T2DM, with the advantages of less impact on blood glucose, simpler operation, and more safety. The last but not the least, the results of arginine stimulation test are consistent with the standard meal test’s, particularly for the first phase insulin secretion. 2015 Vol. 45 (8): 604- [Abstract] ( 1013 ) HTML (0 KB)  PDF (1425 KB)  ( 1779 ) 608 Significance of homocysteine, folic acid and uric acid in diagnosis of cognitive dysfunction WU Tianying, ZHANG Xiong, ZHENG Yongke, WANG Yidong, PAN Jing, CHEN Yan. Objective: To explore the significance of homocysteine, folic acid and uric acid in diagnosis of cognitive dysfunction. Methods: We selected 63 patients with Alzheimer’s disease (AD), 56 patients with vascular dementia (VaD), 40 patients with mild cognitive impairment (MIC), and 45 healthy individuals at same period as control. The levels of homocysteine, folic acid and uric acid were detected in each group. Results: ①The levels of homocysteine in AD group and VaD group were higher than those in MCI group and control group; ②The levels of folic acid in AD group, VaD group, MCI group were lower than those in control group; ③The levels of uric acid in AD group were lower than those in VaD group, MCI group and control group. Conclusion: High levels of homocysteine may be related with the pathogenesis of cognitive dysfunction. Lower levels of folic acid are related with the cognitive dysfunction. The AD patients has lower levels of uric acid. Detecting homocysteine, folic acid and combinated with uric acid is helpful for the diagnosis of cognitive dysfunction. 2015 Vol. 45 (8): 608- [Abstract] ( 787 ) HTML (0 KB)  PDF (1340 KB)  ( 961 ) 611 The applies value of MR susceptibility weighted imaging in the diagnosis of premature infants with intracranial hemorrhage LIN Yizi1, GAO Yuantong1, YAN Zhihan2. Objective: To investigate the value of susceptibility weighted imaging (SWI) in the diagnosis of premature infants with intracranial hemorrhage (ICH). Methods: Sixty cases (26 male, 34 female) of premature infants with suspected ICH were consecutively collected in this study. All the patients were examined by 1.5T MR scanner with conventional MR (T1WI and T2WI), DWI and SWI sequences. SWI sequence was respectively compared with conventional MRI sequences and DWI in detecting intracranial hemorrhage, including the cerebral bleeding, intraventricular hemorrhage, subarachnoid hemorrhage (SAH) and subdural hemorrhage. Results: In sixty premature infants, thirty-nine cases with ICH were detected on SWI, twenty-eight on T1WI plus T2WI, thirty-three on DWI. Intraperenchymal hemorrhagic lesion was detected on SWI in 189 cases, on T1WI-T2WI and DWI in 92 and 111 cases, respectively. Twenty-five intracranial extracerebral (SAH and subclural) and intrarentricular hemorrhagic lesions were detected on SWI, 27 and 25 lesions were detected on T1WI-T2WI and DWI, respectively. For cerebral hemorrhage, there were significant difference between SWI and T1WI plus T2WI, as well as SWI and DWI (P<0.05). For extraxial hemorrhage and intraventricular hemorrhage, no statistical significance was found beween between SWI and T1WI plus T2WI, as well as SWI and DWI (P>0.05). Conclusion: SWI can detect more intracranial hemorrhage than conventional MRI sequence and DWI sequence, which is helpful for diagnosis of intracranial hemorrhage in premature infants. 2015 Vol. 45 (8): 611- [Abstract] ( 620 ) HTML (0 KB)  PDF (1458 KB)  ( 982 ) 615 Association between black tooth stain and primary dentition caries of preschool children in Wenzhou WANG Xiping, CHEN Wenbo, MEI Liqin Objective: To investigate the prevalence of black stain among 5-year-old children in Wenzhou and to assess a possible association between black stain and early childhood caries (ECC). Methods: This cross-sectional study was carried out in six public kindergartens in Wenzhou, China. The study consisted of 718 5-year-old children. Caries statuses of dmft and dmfs were assessed by one calibrated dentist using WHO criteria and black stain was evaluated by the same dentist. x2 test was used to compare the difference of the prevalence of caries between the children with black stain or not. Mann-Whitney U test was used to compare the difference of dmft and dmfs between the children with black stain or not. Results: Black stain was observed in 17.6% of this population. The prevalence, dmft and dmfs of children with black stain were 53.2%, 2.02 and 3.79, fewer than children without black stain (73.1%, 4.45 and 10.14, P<0.05). Conclusion: There is a negative correlation between black stain and caries. 2015 Vol. 45 (8): 615- [Abstract] ( 645 ) HTML (0 KB)  PDF (1328 KB)  ( 970 )

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