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2015 Vol. 45, No. 6 Published: 2015-06-25   391 Histone methylation modification of topoisomerase IIα promoter regulation factors in patients with chronic benzene poisoning SHI Yifen1, ZHENG Zhouyi2, CHEN Jingjing2, QIAN Shanhu2, LI Jiaqi2, YU Kang1. Objective: To investigate histone methylation modification of topoisomerase IIα (TOPO IIα) promoter regulation factors in patients with chronic benzene poisoning, to explore the possible regulatory mechanism of TOPO IIα involved in toxicity of chronic benzene poisoning. Methods: The bone marrow samples were cellected from 25 chronic benzene poisoning cases and 25 normal controls. The Chromatin Immunoprecipitation (ChIP) assay was carried out to study the possible mechanism of TOPO IIα promoter regulation factors expression changes.TOPO IIα promoter regulation factors mRNA were detected by RT-PCR technique. Results: Compared with the normal controls, the histone H3K4 methylation level of TOPO IIα promoter regulation factors NF-M, C-JUN in chronic benzene poisoning patients decreased (P<0.05), while the histone H3K4 methylation level of SP1, ATF-2, SP3, NF-YA, P53, C-MYB, ICBP90 without obvious change (P>0.05). The histone H3K9 methylation level of SP1, NF-M increased (P<0.05 or P<0.01), while the histone H3K9 methylation level of ATF-2, SP3, NF-YA, P53, C-MYB, C-JUN, ICBP90 was no significant change (P>0.05). The mRNA expression of TOPO IIα promoter regulation factors SP1, NF-YA, C-MYB, NF-M and C-JUN were significantly lower than that in the control (P<0.05 or P<0.01), while the expression of SP3, P53 mRNA increased (P<0.05 or P<0.01), ATF-2, ICBP90 mRNA wasn’t changed (P>0.05). Conclusion: In chronic benzene poisoning patients, TOPO IIα promoter regulation factors histone modification changes accompanied with mRNA level changed. Histone methylation modification of topoisomerase enzyme IIα promoter regulation factors play an important role in the benzene’s hematopoietic toxicities. 2015 Vol. 45 (6): 391- [Abstract] ( 717 ) HTML (1 KB)  PDF (1835 KB)  ( 926 ) 397 Effects of extract of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2 in myocardial cells induced by high glucose and its underlying mechanism LI Jianmin, WU Ling, ZHANG Di, ZHENG Jingyu, DAI Shuang, YUAN Fengju, WU Huan, PENG Tianqing. Objective: To investigate the effects of Ginkgo biloba on apoptosis and the expression of Drp-1, Mfn-2, Bax, Bcl-2 and Caspase-3 in mitochondrial apoptotic pathway in neonatal rat myocardial cells induced by high glucose and underlying mechanism. Methods: Left ventricule myocardial cells of neonatal SD rats were isolated and cultured with low-glucose culture media (LG group), low-glucose culture media added EGB (LG +EGB group), high-glucose culture media (HG group) and high-glucose culture media added EGB (HG+EGB group) in vitro respectively. The ratio of apoptosis of myocardial cells was analyzed by flow cytometry. The expression of Drp-1, Mfn-2, Bax, Bcl-2 and Caspase-3 were detected with western blot and/or RT-PCR respectively. Results: Compared to the LG group, the rate of apoptosis, the expression of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2 in ventricule myocardial cells were increased in HG group significantly (P<0.05), while the expression of Mfn-2 and Bcl-2 were decreased significantly (P<0.05). Compared to HG group, the rate of apoptosis, the expression of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2 in HG+EGB group were decreased significantly (P<0.05), while the expression of Bcl-2 was increased significantly (P<0.05). But the expression of Mfn-2 had no significant difference (P>0.05). Conclusion: EGB plays an important role in attenuation cardiomyocyte apoptosis induced by  high glucose via its upregulation the expression of Bcl-2 and down regulation of Drp-1 and Bax, Caspase-3 and the ratio of Bax/Bcl-2. It may be one of the underlying mechanisms for EGB to treat diabetic cardiomyopathy. 2015 Vol. 45 (6): 397- [Abstract] ( 653 ) HTML (1 KB)  PDF (1680 KB)  ( 946 ) 402 Establishment and preliminary analysis on mtDNA-depleted cells and transmitochondrial cybrids WEN Chaowei, YE Wei, ZHOU Huaibin, LV Jianxin, LI Wei. Objective: To generate mtDNA-depleted Hela S3 cells and the transmitochondrial cybrids and investigate mtDNA replication and mitochondrial structure. Methods: Hela S3 cells were incubated with 100 ng/mL ethidium bromide (EtBr). Polymerase chain reaction (PCR) and quantitative real-time PCR (qPCR) were performed in ρ0 Hela S3 cells. Platelet-mediated transmitochondrial cybrids were established on the basis of ρ0 Hela S3 cells and identified by PCR, qPCR and transmission electron microscopy (TEM). Results: ρ0 Hela S3 cells had a low level of mtDNA replication and distorted cristae of mitochondria, whereas transmotichondrial cybrids had a normal mtDNA replication and regular arranged cristae of mitochondria. Conlusion: The generation of transmitochondrial cybrids provides a viable method for the further research of the effect of mtDNA mutations on mitochondrial respiratory function associated with diseases. 2015 Vol. 45 (6): 402- [Abstract] ( 678 ) HTML (1 KB)  PDF (2611 KB)  ( 1371 ) 406 Construction and immune response of recombinant whole-cell yeast expressing HCMV-UL138 CTL epitope ZHANG Yuejin1, LIU Xianyun2, CHEN Xiangmin3, TIAN Xiaojuan4, LI Wenshu4, XUE Xiangyang4 Objective: To construct the recombinant yeast expressing HCMV-UL138 CTL epitope based on the carrier of hepatitis B virus surface antigen (HBsAg), and further explore its immune response. Methods: The complete amino acid sequence of HCMV UL138 was retrieved from the SwissProt Database. The CTL epitopes-riched peptide screened by the methods of SYFPEITHI, Net-CTL and HLA-Bind were inserted into the C-terminal of PreS21-9 sequence, then linked to the N-terminal of HBsAg sequence. The aim sequence optimized according to the yeast cell codon preference was synthesized and cloned into pPIC3.5K vector of yeast expression. The constructed pPIC3.5K/PreS2-HBsAg-UL13815-27 recombinant plasmid was linearized by Bgl II restriction enzyme and electricity transformed into GS115 strain to construct PreS2-HBsAg-UL13815-27 recombinant yeast. Identified recombinant strains were then induced by methanol. The expression of aim protein was identified by Western Blot and ELISA methods using HBsAg antibodies. The identified recombinant yeast cells were inactivated by heat and vaccinated mice by subcutaneous injection. Specific antibodies to HBsAg were detected by ELISA, and cytotoxic T lymphocyte (CTL) response were detected by investigating the interferon-γ (IFN-γ) expression by RT-PCR when immune spleen cells of mice were stimulated by UL13815-27 CTL epitope peptide (VMLVLIVAILCYL). Results: Data of PCR detection, restriction enzyme digestion and sequencing analysis showed that pPIC3.5K/PreS2-HBsAg-UL13815-27 recombinant plasmid was successfully constructed. After induced by the methanol, HBsAg-specific antibodies confirmed the expression of PreS2-HBsAg-UL13815-27 with molecular weight about 33 kD in the lysate of the recombinant yeast. The immunization of inactivated recombinant whole-cell yeast expressing PreS2-HBsAg-UL13815-27 could induce significantly anti-HBsAg-specific IgG antibodies. When the immune spleen cells of mice were stimulated by UL13815-27 CTL epitope peptide, analysis of cellular immune revealed that the production of IFN-γ significantly increased in the group of inactivated recombinant whole-cell yeast expressing PreS2-HBsAg-UL13815-27 than that in the control groups. Conclusion: The recombinant Pichia pastoris yeast expressing PreS2-HBsAg-UL13815-27 protein is successfully constructed. The specific UL138-specific cellular immune response can be induced in the immunized mice with recombinant whole yeast cells. 2015 Vol. 45 (6): 406- [Abstract] ( 602 ) HTML (1 KB)  PDF (1689 KB)  ( 1016 ) 413 Application of vaginal 17β-estradioltion administration in endometrial preparation for patients undergoing frozen-thawed blastocyst transfer CHI Haihong, ZHANG Huina, LU Xiaosheng, XI Haitao, LV Jieqiang Objective: To investigate the relationship between pregnancy and serum estradiol (E2) levels, endometrial thickness, endometrial pattern in patients using vaginal administration of 17β-estradiol in frozen-thawed embryo transfer (FET). And to analyze the effect of using the different dose of 17β-estradiol on serum (E2) levels, endometrial thickness, endometrial pattern and pregnancy rate. Methods: A total of 90 patients of anovulia induced barrenness were undergone frozen embryo transfer in the Reproduction Medical Center of the Second Affiliated Hospital of Wenzhou Medical University from June 2013 to May 2014. The relationship between serum estradiol (E2) levels, endometrial thickness and endometrial pattern was investigated in two groups including pregnant group (n=49) and non-pregnant group (n=41) according to clinical outcome. All the patients were divided into two groups: group A (vaginal estrogen 1 mg) and group B (vaginal estrogen 2 mg). The difenrences of their sesum E2 levels, endometrial thickness, endometrial pattern and pregnancy rate were compared between the two groups. Results: The clinical pregnancy rate was 54.00% (49/90), the implantation rate was 34.71% (59/170). There were no satistical diffefence in patients’ age, infertile years, endometrial thickness of uterus, number of embryos transferred, E2 and P levels on the day injection of progesterone and on transplant day between pregnant group and non-pregnant group. The proportion of type A endometrium in pregnant group were significantly higher than that in non-pregnant group (P<0.05). E2 levels on injection of progesterone, E2 levels on transplant day were significantly lower in group A compared with group B (P<0.05). No significant difference were shown in the mean of endometrial thickness of uterus, endometrium patterns, P levels ontransplant day, the clinical pregnancy rate and the implantation rate. Conclusion: Our study shows that it is suitable for patients with anovuliain frozen embryo transfer cycle through hormone supplyment therapy with vaginal administration of 17β-estradiol, and the serum E2 levels, endometrial thickness and clinical pregnancy rates could achieve satisfactory results.estrodiol and dydroesterone of femoston in FET cycle. 17β-estrodial 1mg can reach to 2 mg of endometral thickness and pregnancy rate. The protocol is simple and feasibility. The endometrium pattern is one of the important factorsaffect pregnancy rates.   2015 Vol. 45 (6): 413- [Abstract] ( 660 ) HTML (1 KB)  PDF (1346 KB)  ( 887 ) 417 Phenotype change and correlative analysis for a combined inherited factor VII and factor X deficiency due to accidental ingestion of a diphacinone rodenticide XIE Yaosheng, JIN Yanhui, XIE Haixiao, YANG Lihong, ZHU Liqing, WANG Mingshan. Objective: To explore the characteristics of laboratory phenotype and correlative analysis for a patient with combined inherited FVII and FX deficiency after he had ate a diphacinone rodenticide accidentally.  Methods: The coagulant parameter screening tests and coagulation factors activities were detected for many times in the patient due to accidental ingestion of a diphacinone rodenticide. After the patient was treated for more than one year, gene analysis of correlated coagulation factors was analyzed in the patient and other family members with DNA direct sequencing. Select 106 persons of health examination as control. Results: After the patient was admitted to hospital, routine coagulation screening tests revealed the prolonged prothrombin time (PT) and activated partial thromboplastin time (APTT) and low levels of vitamin K-dependent coagulation factors (FII, FVII, FIX, FX) activity, which was 102.4 s, 88.5 s, 7%, 3%, 8% and 2%, respectively. During more than one year of treatment, the value of PT and APTT still showed significantly prolonged, FVII and FX activity levels were about 5%. While FII and FIX activity levels were in the normal range after 12 weeks of treatment. Two homozygous mutations, g.11267C>T of F7 gene resulting in the substitution Arg277Cys and g.28139G>T of F10 gene leading to the substitution Val384Phe, were identified in the patient. The patient’s parents and sister were heterozygous for Arg277Cys and Val384Phe mutations. FVII and FX antigen levels in the patient were 7% and 30%, respectively. Conclusion: There are many similarities in the characteristics of laboratory examination between combined inherited FVII and FX deficiency and acquired vitamin K deficiency. The best way to identify them is gene analysis. 2015 Vol. 45 (6): 417- [Abstract] ( 641 ) HTML (1 KB)  PDF (1501 KB)  ( 1002 ) 421 Effects and mechanisms of novel chalcone analogue-A59 on inducing colon cancer cells’ apoptosis SUN Yusheng1, ZHU Guanbao2, JIN Jinji2, TAO Lijun1, ZHU Weixing1, FANG Jun1. Objective: To further explore its anti-tumor activity and mechanisms through investigation of the signal path of novel chalcone analogue-A59. Methods: Synthesize the chalcone analogues and further test their cytotoxicity on colon cancer cell line (HCT-116) through MTT method, and pick out the one own better biological activity named A59. The ability of A59 on inducing HCT-116’s apoptosis and inhibiting its cell cycle were assayed using FCM method. The effect of A59 on ATF4 and CHOP expression in HCT-116 were assayed with Western blot. After knockdown CHOP by siRNA technology, the influence of A59 on cell’s apoptosis through immunofluorescence (IF) and FCM was further investigated. Results: A59 showed inhibitory effects on the proliferation of HCT-116 (IC50=7.6 μm). This novel chalcone analogue exerted its biological activity to induce HCT-116’s apoptosis and inhibit its cell cycle through FCM testing, and the Western blot assay indicated that A59 showed a dose-dependent activation of key protein CHOP of endocytoplasmic reticulum stress (ERS) signal path. and the siRNA technology further confirmed A59 induce apoptosis by stimulating the CHOP over-expression. Conclusion: Novel chalcone analogue-A59 shows inhibitory effects on the proliferation of HCT-116 by activating the ERS signal path’s key protein CHOP. Clarifies its mechanisms and shows the significance on the new anti-colon cancer drug research and development. 2015 Vol. 45 (6): 421- [Abstract] ( 685 ) HTML (1 KB)  PDF (2921 KB)  ( 1021 ) 426 The clinical application of percutaneous transluminal balloon angioplasty for hemodialysis patients with stenosis of arteriovenous fistula ZHENG Chenfei1, SUN Wenxue1, JIA Xiufen2, MIAO Kekai3, PAN Huijun3, LIU Yi1, HUANG Chaoxing1. Objective: To report the technical success and long-term clinical outcomes of arteriovenous fistula (AVF) stenosis after percutaneous transluminal balloon angioplasty (PTA). Methods: A total of 30 patients with stenosis of AVF were enrolled. There were 16 men and 14 women, and the median age was 64.8±13.2 years. Mean time between fistula creation and intervention was 16.5±13.8 months. The mean duration of hemodialysis time was 31.5±30.2 months. They were examined by digital subtraction angiography (DSA) when they underwent PTA. After the treatment of PTA, all of the patients were examined by 64-multislice spiral computed tomography angiography every 3 months. Results: The technical success rate was 90.0% in the hemodialysis patients (17 native AVFs and 10 arteriovenous grafts). Primary patency at first, second, third, fourth and fifth three months was 100%, 91.3%, 79.5%, 72.6% and 62.2% respectively. Univariate analysis indicated that there was no significant correlation between the clinical factors (including age, diabetes mellitus, location, length, grade, and number of stenosis) and primary patency (P>0.05). Conclusion: PTA is an effective treatment method for arteriovenous stenosis, which has high rates of technical success and less complication. 2015 Vol. 45 (6): 426- [Abstract] ( 656 ) HTML (1 KB)  PDF (1548 KB)  ( 1130 ) 430 The epigenetic alteration and function of PLAGL1 in colorectal cancer XIANG Zhenfei, LU Miaozhen, ZHANG Huanle, ZHANG Sandian. Objective: Epigenetic alterations have played an important role in colorectal carcinogenesis. PLAGL1 (pleiomorphic adenoma gene-like 1, Also known asZAC; LOT1; ZAC1) is a gene associated with the development of many types of cancers. Our aims are to explore the expression and the function of PLAGL1 in colorectal cancer. Methods: RT-PCR (semi-quantitative reverse-transcription PCR) was used in this study to detect the expression level of PLAGL1 in colorectal cancer cell lines. The methylation of PLAGL1 promoter regain was detected with MSP (methylation specific PCR) in colorectal cancer cell lines and colorectal cancer tissues. MTT assay, colony formation and cell cycle analysis were used to explore the function of PLAGL1 in colorectal carcinogenesis. Results: The expression of PLAGL1 in colorectal cancer cell lines was regulated by promoter regain methylation. And PLAGL1 was frequently methylated in 64.4% (67/104) colorectal cancer tissue. Restoration of PLAGL1 expression in HCT116 inhibited cell proliferation and colony formation, and cell cycle of HCT116 was arrested at G1 phase. Conclusion: The expression of PLAGL1 in colorectal cancer is regulatied by DNA methylation.The restoration of PLAGL1 can suppressed the proliferation of colorectal cancer cell HCT116, and also arrest the cell cycle of HCT116 in G1 phase. PLAGL1 acts as a tumor suppressor in colorectal carcinogenesis. 2015 Vol. 45 (6): 430- [Abstract] ( 681 ) HTML (1 KB)  PDF (2642 KB)  ( 896 ) 434 Correlative study on the rapid-eye-movement sleep behavior disorder and cognitive impairment of Parkinson’s disease ZENG Tingting, WU Zhiping, ZENG Min, QIU Weiwen. Objective: To investigate the correlation between rapid-eye-movement sleep behavior disorder (RBD) and the cognitive impairment in patients with Pakinson disease (PD). Methods: Fifty-four patients with Parkinson disease were enrolled from Neurology Department of Lishui Central Hospital from 2012 to 2013 (25 PD-RBD cases and 29 PD-NRBD cases), with 21 healthy participants as the control group. Full-night video- polysomnography (vPSG) for two nights, Unified Parkinson Disease Rating Scale (UPDRS-III) Questionaire, Hoehn-Yahr stage criteria, Montreal Cognitive Assessment (MoCA) and The Epworth Sleepiness Scale (ESS) were performed on all study participants. Results: The score for attention and calculation, visual-spatial and its congitive function, delayed memory in PD-RBD group were all significantly lower than that in PD-NRBD group and control group (P<0.05). Sleep efficiency in both PD-RBD and PD-NRBD group were lower than that in control group, and often accompanied by obstructive sleep apnea-hypopnea syndrome and restless leg syndrome (P<0.05). There was no statistical significant difference between the two PD groups (P>0.05). Conclusion: Parkinson disease patients combined with RBD are correlated with cognitive dysfunction. RBD can be used as a predictor for cognition impairment in Parkinson disease. 2015 Vol. 45 (6): 434- [Abstract] ( 706 ) HTML (1 KB)  PDF (1307 KB)  ( 946 ) 437 Monitor of air borne hospital environment by Illumina high-throughput sequence LI Yanming1,6, TONG Xunliang2, XU Hongtao3, TIAN Geng4, LANG Jidong4, XIAO Fei5, CAI Meng6. Objective: To monitor microorganisms in air borne environment in hospital and to analysis the distribution characters of microorganisms in different departments in hospital. Methods: Air samplers were utilized in four typical departments of hospital for airborne microorganisms’ collection. Microorganisms were identified by DNA sequencing analysis by Illumina MiSeq and HiSeq 2000 sequencing systems. Results: The airborne microorganisms from four departments were analyzed for microbiome sequencing and classified as fungi,species, bacteria species and viruses species. The ratio of pathogenic fungi in fungi was different in four departments and the ratio in two intensive care units were higher than its in outpatient department and emergency department. At the species level of fungi, ratio of yeast, mycotic fungus and dimorphic fungi in different department was shown. The relative ratio of aspergillus in mycotic fungus and the relative ratio of cryptococcus and candida in yeast was shown,respectively. Conclusion: Novel method was utilized in monitoring the air borne microorganisms in hospital environment and the relative ratio of common opportunistic pathogenwas satisfied. It is provided experiment based information in improving the methodology in monitoring hospital infection. 2015 Vol. 45 (6): 437- [Abstract] ( 743 ) HTML (1 KB)  PDF (2418 KB)  ( 1294 ) 440 Clinical efficacy of exenatide with insulin detemir treatment in obese patients with type 2 diabetes XIA Fang, YE Liying, A Rong, HU Yongmei Objective: To investigate the clinical efficacy of exenatide combined with insulin detemir in newly diagnosed obesity patients with type 2 diabetes. Methods: 22 cases of newly diagnosed obesity patients with type 2 diabetes were choosed, who had been treated with exenatide and metformin for 4 weeks and their fasting blood glucose (FBG) were higher than the control target. They were treated with exenatide, metformin and insulin detemir for 8 weeks to observe the changes of blood glucose and weight. Results: After combined insulin detemir for 8 weeks, the fasting blood glucose was controlled well and the 2 hours postprandial blood glucose and weight were decreased continuously. Conclusion: The therapy with exenatide and  insulin detemir can be used by obese patients with type 2 diabetes. 2015 Vol. 45 (6): 440- [Abstract] ( 991 ) HTML (1 KB)  PDF (1334 KB)  ( 829 ) 443 Related risk factors of acute pancreatitis followed by adult congenital biliary dilatation GUAN Huaqin1, XIE Saili2, SUN Xuecheng1, CHEN Renpin1, CHEN Chao1, WU Jianshen1. Objective: To identify the potential risk factors of congenital biliary dilatation (CBD) complicated with acute pancreatitis (AP) in adults. Methods: A total number of 96 patients that diagnosed as CBD in our hospital from February 2002 to April 2014 were retrospectively reviewed, which were divided into two groups, CBD with AP (AP group) and CBD without AP (NAP group). Univariate and multivariate Logistic analysis were conducted to evaluate risk factors complicated with AP. Results: In univariate analysis, significant differences were found between these two groups by biliary calculi, ALT, AST (P<0.05). In logistic regression analysis, only biliary calculi was recognized as the significative factor (P<0.05). CBD combined bile duct stones of group A (Merge large stones A1 group, Merge small stones A2 group); Simple biliary calculi as control group B (large stones B1 group, small stone B2 group). Incidence of acute pancreatitis in A2 group is highest and is significantly higher than that of B2 group and A1 group (P<0.05). Conclusion: Biliary small stones with adult CBD in patients is a risk factor of AP. 2015 Vol. 45 (6): 443- [Abstract] ( 602 ) HTML (1 KB)  PDF (1337 KB)  ( 844 ) 446 Observation on the curative effect of crown lengthening surgery with piezosurgery BU Zhongqi1, GU Haijia1, YAO Siqi2. Objective: To evaluate the effects of piezousurgery and high-speed burr in crown lengthening surgery. Methods: Sixty seven teeth in 60 examples with subgingival tooth defect but less than 4 mm, that got the value to preserve were selected. The examples are divided into two groups randomly. One group of 34 teeth in 30 examples used piezosurgery. The other group of 33 teeth in 30 examples used the high-speed burr. The defect of the lowest probing depth (PD), sulcular bleeding index (SBI), tooth mobility (TM) were recorded before operation, at one week after operation, 8 weeks after operation and 6 months after operation. Results: The after-operation PD and SBI of both group had significant changes. The difference was statistically significance (P<0.05), while comparison between the two groups, there’s no statistical significance in the difference of PD and SBI one week after operations (P>0.05), and there lies significant difference in PD 8 weeks after operations (P<0.05), but SBI had no marked difference, and all indexes after 6 months were the same as these after 8 weeks. Conclusion: These two kinds of operations both have good effect on patients who need crown lengthening surgery to recover the subgingival tooth defects. At the same time, piezosurgery causes less bone loss, smaller periodontal wound and the better recovery. 2015 Vol. 45 (6): 446- [Abstract] ( 610 ) HTML (1 KB)  PDF (1335 KB)  ( 1009 ) 449 The value of computed tomography perfusion imaging in differential diagnosis for inflammatory nodule and lung cancer  FU Gangze, CHEN Yongchun, XIE Furong. Objective: To evaluate the value of computed tomography perfusion imaging in distinguishing inflammatory and malignant nodules. Methods: CT perfusion scans were performed in patients with lung nodule received CT perfusion between September 2010 and February 2013. Patients with lung cancer and/or inflammatory nodule were confirmed by operation and proved by pathology. The parameters of CT perfusion, including blood volume (BV), blood flow (BF), permeability-surface area product (PS), mean transit time (MTT) were produced by perfusion software, and peak high (PH), time to peak (TTP), PHSPN/PHAA were measured. CTP parameters between above subgroups were analyzed using Mann-Whitney test. Results: Thirty-two cases were effective, including 26 cases of lung cancer and 6 cases of inflammatory nodule. Inflammatory nodules had higher BF, PH, PHSPN/PHAA than lung cancers (P<0.05). SPNS with BF larger than 78 mL • min-1 • 100 g-1 or PHSPN/PHAA larger than 8.5% were more likely to be inflammatory (sensitivity of 88.5%, specificity of 100%, PPV of 100%, NPV of 66.7% and accuracy of 90.6% for BF; sensitivity of 80.8%, specificity of 83.3%, PPV of 95.5%, NPV of 50.0% and accuracy of 81.3% for PHSPN/PHAA). Conclusion: The CT pulmonary perfusion imaging is of some differential role in distinguishing the inflammatory nodule and malignant nodule. 2015 Vol. 45 (6): 449- [Abstract] ( 670 ) HTML (1 KB)  PDF (1425 KB)  ( 919 )

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