温州医科大学学报
 
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2015 Vol. 45, No. 4
Published: 2015-04-25
 
 
235 Investigation of ST6Gal I on the ability of proliferation and migration in SGC7901 gastric cancer cell
ZHU Menglu, LIU Tingting, PENG Xiaodan, LIN Shaoqiang
Objective: To explore the effect of α2, 6 sialytransferase (ST6Gal I) on the abilities of proliferation and migration in SGC7901 cells. Methods: Gastric cancer cell lines SGC7901 were constructed to ST6Gal I high expression (PS7) and ST6Gal I lower expression (X61) with lipofectamine 2000 and empty vector (Vector) was used as a control group. The expression of ST6Gal I was examined with RT-PCR. Cell proliferation was evaluated with the CCK-8 kit, and migration was evaluated with Transwell chamber assay. Results: The 3 stable transfected cell lines were constructed successfully. The most effective clone cells were chosed as our experimental cells, and named as Vector, X61-4 and PS7. In contrasted with Vector, the expression of ST6GAL I in X61-4 was statistically significant lower (P<0.05), and PS7 was statistically significant higher (P<0.05). Cell growth curve showed that cell proliferation of X61-4
was obviously higher than that of PS7 cells (P<0.05). The transwell chamber assay results showed that the migration of PS7 was significantly higher than that of the X61-4 (P<0.01) and Vector (P<0.01), X61-4 was lower than that of the Vector (P<0.05). Conclusion: Cell experiment results show that lower ST6Gal I can effectively promote the proliferation ability of SGC7901 gastric cancer cells, but high ST6Gal I can enhance gastric cancer cell migration ability. Therefore, ST6Gal I is related to the tumor proliferation and metastasis, and it is also a potential tumor molecular therapeutic targets.
2015 Vol. 45 (4): 235- [Abstract] ( 617 ) HTML (1 KB)  PDF (5125 KB)  ( 1072 )
240 Cloning of three newly-identified alternatively splicing isoforms of mouse Pax-8 and their spatial and temporal expression patterns
SONG Lijuan1, NI Yunjie1, LIN Zhenhao1, HOU Lianglei1, WU Yihao1, HU Huanhuan1,
Objective: Pax-8 gene plays a pivotal role in embryonic development and tumorgenesis. This study aims to identify and clone different alternatively splicing isoforms of mouse Pax-8, and to detect their spatial and temporal expression patterns. Methods: Hearts from wild-type prenatal and postnatal mice of C57BL/6 background were carefully excised and total RNA was extracted using Trizol reagent. The cDNA fragment of Pax-8 was reverse transcribed and amplified by Q5 Taq polymerase and cloned into pcDNA3.1(-) vector. The recombinant vectors were validated by enzyme digestion and sequencing. Results: In comparison to their cognate full-length Pax-8a, three alternatively splicing isoforms of mouse Pax-8, designated Pax-8b (exon4 deleted), Pax-8c (exon 4 and 11 deleted) and Pad-8d (exon 4 and 9 deleted), were identified in different periods of embryogenesis and successfully cloned. Conclusion: Mouse heart has four alternatively splicing isoforms of mouse Pax-8 that express in different spatial and temporal expression patterns. Such findings open a bright avenue for the further functional study.
2015 Vol. 45 (4): 240- [Abstract] ( 611 ) HTML (1 KB)  PDF (7508 KB)  ( 955 )
243 Effect of Sedum sarmentosum Bunge Extract on TGF-β1-induced renal epithelial-to-mesenchymal transition and matrix accumulation
YANG Rongrong1, LU Hong1, WU Lianfeng1, WANG Silu2, LIN Chengcheng2,
Objective: To investigate the protective effects of Sedum sarmentosum Bunge Extract (SSBE) on TGF-β1-induced epithelial-to-mesenchymal transition (EMT) and matrix accumulation in renal tubular epithelial cells (NRK-52E). Methods: NRK-52E cells were divided randomly into: control group treated with only solvent, TGF-β1 group treated with TGF-β1 at the concentrations with 5 µg/L and SSBE group treated with TGF-β1 plus SSBE at the concentrations with 10 and 100 mg/L. The morphology of the NRK-52E cells was observed under inverted/phase contrast microscope. Immunofluorescent analysis was performed to detect the expression of epithelial marker α-smooth muscle actin (α-SMA), mesenchymal marker E-cadherin, and matrix component type III collagen. Gene expression of α-SMA, bone morphogenic protein-7 (BMP-7), tight junction protein-1 (Tjp1), Col1a1, Col3a1, MMP-2, and TIMP-2 were also quantified by real-time RT-PCR. Results: In TGF-β-treated NRK-52E cells, fibrosis-like phonotype was obviously increased. TGF-β1 increased the expression of α-SMA, and decreased the expression of E-cadherin and Tjp1. Also, TGF-β1 enhanced the expression of type I and III collagens. Treatment with SSBE at the concentrations with 10 and 100 mg/L inhibited TGF-β1-induced fibrosis-like phenotype of NRK-52E cells, accompanied with down-regulated expression of α-SMA, Col1a1 and Col1a1 and up-regulated expression of E-cadherin and Tip1. In addition, SSBE increased the expression of BMP-7 and the ratio between MMP-2 and TIMP-2. Conclusion: SSBE treatment reduce TGF-β1-induced fibrosis-like reaction in renal tubular epithelial cells through inhibiting EMT and matrix accumulation.
2015 Vol. 45 (4): 243- [Abstract] ( 621 ) HTML (1 KB)  PDF (18820 KB)  ( 620 )
248 Analysis of factors affecting lymph node metastasis in FIGO IB to IIB uterine cervical cancer
CHEN Huijun1, HUANG Yibo1, LIN Rongrong1, ZHENG Feiyun1.

Objective: To analysis the factors affecting lymph node metastasis in stage IB1 to IIB uterine cervical cancer. Methods: A total of 158 patients with stage IB to IIB cervical carcinomas treated with radical hysterectomy and systematic pelvic lymphadenectomy were retrospectively analyzed. The logistic multivariate analysis was used to select independent high-risk factors. Results: Thirty-six (22.8%) patients had pelvic lymph node metastasis. Of 36 lymph node metastases, 22 were in the obturator, 15 in the internal iliac, 10 in the external iliac, 7 in the common iliac, 4 in the deep inguinal, 1 in the parametrial iliac and 1 in the para aortic. Univariate analysis revealed that tumor size, preoperative SCC-Ag level and parametrial invasion were related to pelvic lymph node metastasis. Multivariate analysis revealed that tumor size greater than 4 cm and parametrial invasion were independently associated with nodal metastasis. Conclusion: The obturator lymph nodes are most frequently involved in stage IB～IIB uterine cervical cancer. Tumor size greater than 4 cm and parametrial invasion are high-risk factors of pelvic lymph node metastasis.
2015 Vol. 45 (4): 248- [Abstract] ( 705 ) HTML (1 KB)  PDF (1281 KB)  ( 1065 )
252 The effect and the mechanism of Roxithromycin on apoptosis of airway smooth muscle cells from asthmatic rats in vitro
WU Haiya1, DAI Yuanrong2, YING Binyu1.
Objective: To observe the effects of Roxithromycin on apoptosis of airway smooth muscle cells (ASMCs) in vitro and the role of mitochondrial pathway in apoptosis of ASMCs. Methods: ASMCs from asthmatic rats were cultured in vitro and intervened with various concentrations of Roxithromycin for 48 hours. Early apoptotic cells were detected through flow cytometry (FCM) after stained with Annexin V/PI; Alteration of mitochondrial membrane potential (ΔΨm) was also examined by FCM after stained with JC-1; Cytochrome C (Cyt-c)
both in mitochondria and cytoplasm was analyzed by Western Blot. Results: Roxithromycin could induce the apoptotic death of ASMCs in vitro: Early apoptotic rate in experimental group (R10, R25, R50, R100) after 48 h intervention was (4.6±1.9)%, (5.8±2.9)%, (12.0±5.6)% and (26.9±11.1)%, respectively, compared to control (2.9±1.7)%. And there was a significant difference (x2=13.30, P<0.01) between the group R100 and the control.RXM induced loss of ΔΨm: The proportion of low ΔΨm cells of the experimental group (R10, R25, R50, R100) was (27.88±13.10)%, (40.35±9.19)%, (48.40±14.15)% and (52.90±15.88)%, respectively, compared to the control group (19.78±9.85)%. The indexes of group R100 and R50 were significantly lower than that of group R0 and R10 (P<0.05 or 0.01). The difference between group R25 and R0 was also statistically significance (P<0.05).But there was no statistical difference between group R10 and R0. Roxithromycin could induce Cyt-c release from mitochondria into the cytosol: The content of cytosolic Cyt-c in group R10, R25, R50, R100 was respectively (0.87±0.19), (0.97±0.17), (1.01±0.12), (1.14±0.07), higher than that in the control group (0.67±0.12). There was a significant difference between the group R100 and R0 (x2=9.075, P<0.05). Conclusion: Roxithromycin can induce apoptosis of ASMCs in vitro via activating mitochondrial pathway.
2015 Vol. 45 (4): 252- [Abstract] ( 596 ) HTML (1 KB)  PDF (7974 KB)  ( 745 )
256 The role of microRNA-101a and cycloxygenase-2 in kidney damage induced by hyperuricemia
YE Hanyang1, JIN Jian2, LI Zhanyuan1, CHEN Yan1, ZHENG Yu1, JIN Lingwei1, ZHOU Zhihong1.
Objective: To investigate the effects of miR-101a and cycloxygenase-2 (COX-2) on rats model of urate nephropathy. Methods: Twenty four rats models of urate nephropathy through fed with yeast and adenine for 21 days were divided into three groups, normal control groups, model groups and celecoxib groups. Serum creatinine (Cr), blood urea nitrogen (BUN) and blood uric acid (UA) was tested after feeding with yeast and adenine for 21 days. The real time PCR was preformed to analyze the expression of miR-101a and Western Blot was preformed to analyze the expression of COX-2 and caspase-3 in renal cortex. Results: Cr, BUN and UA of model group were significantly higher than that of normal control groups (P<0.05). The expression of miR-101a in renal cortex from model groups was lower than that of normal control groups (P<0.05), while higher than that of celecoxib groups (P<0.05). The expression of COX2 and caspase-3 in renal cortex from model groups were higher than that of normal control group (P<0.05), while lower than that of celecoxib groups (P<0.05). Conclusion: Decreasing miR-101a and increasing COX2 may involve in kidney damage induced by hyperuricemia, celecoxib as a specific inhibitor protects kidney from this damage.
2015 Vol. 45 (4): 256- [Abstract] ( 680 ) HTML (1 KB)  PDF (2704 KB)  ( 947 )
260 The antidepressant effect of procyanidins extract from Zanthoxylum bungeanum Maxim on ovariectomized model mice
CHEN Songfang, LI Lifeng, CHEN Jing, WANG Xiaotong, HUANG Hanjin.?
Objective: To explore the antidepressant effect of chronic pre-administration with procyanidins extract from Zanthoxylum bungeanum Maxim (ZPPC) on ovariectomized model mice and its possible mechanism. Methods: The animals behavioral despair models of depression, including the tail suspension and forced swimming tests, were used to explore the antidepressant effects of ZPPC. The locomotor activity test was used to detect the change of locomotor activity. Meanwhile, the monoamine oxidase activity in the mouse brain was also determined. Results: The results showed that ZPPC (50, 100, 200 mg/kg) significantly reduced the duration of immobility in both tail suspension and forced swimming tests. The effective doses did not alter locomotot activity,
resembling the selectivity of the positive control drug fluoxetine (10 mg/kg) in relieving depression. Moreover, ZPPC was found to inhibit monoamine oxidase activity in the mouse brain. Conclusion: These findings suggest that ZPPC exerts antidepressant effect in animals behavioral despair tests and the underlying mechanism may involve the central monoaminergic systems.
2015 Vol. 45 (4): 260- [Abstract] ( 583 ) HTML (1 KB)  PDF (1759 KB)  ( 900 )
265 Application of quantitative analysis of thoracic CT in the diagnosis of severe acute pancreatitis complicated with lung injury
ZHENG Yongfei, WEI Peiying, KONG Jianguo, ZHANG Hua, WU Enfu.
Objective: To explore the early diagnostic value of quantitative index of thoracic CT in severe acute pancreatitis complicated with ALI/ARDS. Methods: Sixty-nine cases of severe acute pancreatitis were collected, 34 cases complicated with ALI/ARDS. The thickness of bilateral lung consolidation, pleural fluid and thoracic cavity were measured and the ratio of them on CT mediastinal window was calculated, respectively, and then evaluate their diagnostic value for ALI/ARDS. Results: There were statistical significance of difference between SAP complicated with ALI/ARDS and SAP upon every quantitative index (the thickness ratio of left lung consolidation to thoracic cavity, the thickness ratio of left lung consolidation to pleural fluid, the thickness ratio of left pleural fluid to thoracic cavity, the thickness ratio of right lung consolidation to thoracic cavity, the thickness ratio of right lung consolidation to pleural fluid and the thickness ratio of right pleural fluid to thoracic cavity). ROC curve showed that three indexes (the thickness ratio of left lung consolidation to thoracic cavity, the thickness ratio of left lung consolidation to pleural fluid and the thickness ratio of consolidation of right lung to thoracic cavity, with positive likelihood ratio of 5.158, 3.970 and 3.530) had greater diagnostical value for SAP complicated with ALI/ARDS than others. With these three indexes, the sensitivity was 0.994 by parallel test. Conclusion: Quantitative indexes of thoracic CT have important reference for early diagnosis of SAP complicated with ALI/ARDS and can provide proof for early therapy.
2015 Vol. 45 (4): 265- [Abstract] ( 633 ) HTML (1 KB)  PDF (5142 KB)  ( 846 )
269 The biological impaction of morphine on lung cancer A549 cells induced by cisplatin
WANG Qiandong1, WU Shujuan2, ZHU Guowen1.
Objective: To analyze the effect and mechanism of the proliferation inhibition and apoptosis in human lung carcinoma after using morphine combined with cisplatin chemotherapy. Methods: Human lung adenocarcinoma cell line A549 was cultured in conventional 1 640 medium. Cell proliferation activity for cisplatin alone or in combination with morphine after treatment for 6 h, 12 h, 24 h and 48 h were detected by CCK8. In addition, Western Blot electrophoresis was adopted to detect cisplatin alone or in combination with the optimal concentration of morphine after 48 h. Then changes of apoptosis related proteins Bax, Bcl-2 Caspase-3, survivin expression level were analyzed. Results: After 24 h, or 48 h, the concentration of 500 μg/mL morphine in combination with cisplatin group, compared to morphine and cisplatin alone group, could significantly increase cancer cell proliferation inhibitory effect (P<0.05). While combined with low-dose concentrations of morphine-treated group, compared with cisplatin alone, the difference was not statistically significant. By WB experiments, we found that the combination of drug treatment could inhibit Bcl-2, survivin protein expression induced by cisplatin, while enhancing the pro-apoptosis death proteins Bax, Caspase-3 expression. Conclusion: The best choice for morphine dosage can significantly enhance proliferation and apoptosis function of cisplatin on lung cancer cell. This may inhibit the proliferation of lung cancer cells by promoting key apoptotic protein Bax and Caspase-3 expression pathway.
2015 Vol. 45 (4): 269- [Abstract] ( 658 ) HTML (1 KB)  PDF (1995 KB)  ( 960 )
273 Effect of aspirin plus clopidogrel on platelet function and prognosis in patients with acute cerebral infaction
CHI Wanzhang1, ZHOU Qiang1, LIN Jing1, YI Xingyang2.
Objective: To evaluate the effects of clopidogrel combined with aspirin compared to aspirin alone on platelet function and short-term outcome in patients with acute large-artery atherosclerosis stroke. Methods: Altogether 570 patients with acute (≤72 h) atherosclerotic cerebral infarction were randomly assigned to receive combined clopidogrel with aspirin and aspirin alone. Platelet aggregation and platelet-leukocyte aggregation were detected at day 1 and day 30. Primary outcome was neurological deterioration, second outcomes including recurrent ischemic stroke, periphery vascular events, myocardial infarction and all cause deaths. Safety endpoints were hemorrhagic episodes and death in 30 days. Results: Patients who received the combination therapy had significantly fewer ischemic stroke recurrence events and neurological deterioration than those given monotherapy. At day 30 of treatment, the platelet aggregations and platelet-leukocyte aggregation were lower than those patients given aspirin alone (P<0.01). Compared to patients who did not suffer neurological deterioration or recurrent stroke, the platelet aggregation induced by arachidonic acid or ADP, and platelet-leukocyte aggregation, at day 1 were higher than those patients who did experience these afflictions (P<0.01). Conclusion: Treatment with combined clopidogrel and aspirin provides significantly greater inhibition of platelet activity than did aspirin alone, and appears to be safer and more effective in reducing ischemic stroke recurrence and neurological deterioration.
2015 Vol. 45 (4): 273- [Abstract] ( 656 ) HTML (1 KB)  PDF (1264 KB)  ( 1389 )
278 Preparation and in vitro quality evaluation of a small type of compound rapamycin suppositories
ZHOU Weizhong1, FU Hongxing2, WU Lanlan1, LIU Chengyang2, LIAO Li2, HUANG Binjiong2.
Objective: To develop a small type of suppository of immunosuppressive agents to suit for large animals after abdominal organ transplantation, and carry out it’s quality evaluation. Methods: Rapamycin and tacrolimusas were chosed as active pharmaceutical ingredients, and polyethylene glycol 2000, polyethylene glycol 4000 were used as substrate. Melting followed with a quantitative injection molding process was used to prepare the small type of compound suppository. The appearance, weight difference, melting time and drug content of the suppository were evaluated. Results: The compound rapamycin suppository was translucent, white, and had good hardness at room temperature. It’s short, and the weight variation, melting time and drug content were conformed to the requirements of the suppository. Conclusion: The compound rapamycin suppository prepared by this method conforms to the quality of suppository, which makes a good foundation for the development of the new drug.
2015 Vol. 45 (4): 278- [Abstract] ( 561 ) HTML (1 KB)  PDF (2376 KB)  ( 939 )
281 Serum secretory Klotho is associated with coronary atherosclerosis in patients on maintenance hemodialysis
ZHENG Shubei, JIN Lingwei, ZHOU Zhihong
Objective: To determine the relationship between serum secretory Klotho and coronary atherosclerosis in maintenance hemodialysis (MHD) patients. Methods: One hundred and fifty-two cases of MHD patients, who were underwent coronary artery angiography, were collected prospectively. Serum secretory Klotho was detected by ELISA. Coronary artery lesions involving the count and Syntax score were recorded by the result of coronary angiography examination. Logistic regression analysis was used to determine the risk factor of coronary atherosclerosis in MHD patients. The ROC analysis was applied to evaluate the diagnostic value of secretory Klotho in coronary atherosclerosis. Results: Eigty-one patients had coronary atherosclerosis. Serum secretory Klotho concentration was negatively correlated with coronary atherosclerosis (r=-0.260, P=0.001). Secretory Klotho and counts of coronary artery lesion showed a negative correlation (r=-0.268, P=0.001). Secretory Klotho was also negatively correlated with Syntax score (r=-0.223, P=0.046). Logistic regression analysis showed that lower secretory Klotho level was an independent risk factor for coronary atherosclerosis (OR=3.125, 95%CI 1.298～7.522). ROC-AUC of serum secretory Klotho for coronary atherosclerosis was 0.650 (cut off 373.825 pg/mL, accuracy 81.7%, specificity 44.4%). Conclusion: Serum secretory Klotho of MHD patients is associated with coronary atherosclerosis, and is negatively related to the severity of coronary atherosclerosis, also is an independent risk factor in MHD patients with coronary atherosclerosis. Serum secretory Klotho may have diagnostic value for coronary atherosclerosis in MHD patients, and can judge the severity of coronary artery lesions.
2015 Vol. 45 (4): 281- [Abstract] ( 608 ) HTML (1 KB)  PDF (1510 KB)  ( 942 )
286 Analysis of characteristics of non-Helicobacter pylori and non-NSAID peptic ulcer disease
ZHENG Endian1, NI Shuangshuang2, LIN Xizhou1, LIN Xiaoxiao1, YE Cici1, CHEN Yan1, YU Yingcong1, ZHENG Liang1
Objective: To expplore the characteristics of non-Helicobacter pylori (Hp) and non-NSAID peptic ulcer disease. Methods: A total of 473 cases with peptic ulcer disease were recruited in the study between 2011 and 2013. Hp status was assessed by histology and/or 13C-urea breath test. These cases were divided into group of non-Hp and non-NSAID peptic ulcer disease (group A), group of Hp positive only (group B), group of NSAID only (group C) and group of Hp positive and NSAID (group D). The proportion of non-Hp and non-NSAID peptic ulcer disease (group A), Gender and ages of these patients were collected. The characteristics of group A, group B, group C and group D were analyzed and compared. Results: Fifty six patients with non-Hp and non-NSAID peptic ulcer disease (group A) were diagnosed and the proportion was 11.8% (56/473). There was no significant difference between group A and other groups in distribution of gender (P>0.05). There was significant difference between group A and group B in distribution of age (P<0.05). There was no significant difference between group A and other groups in clinical symptoms, such as abdominal pain, abdominal distension and so on (P>0.05). There was significant difference between group A and group D in gastrointestinal bleeding (P<0.05).There was significant difference among group A,group B, group C and group D in abdominal tenderness (P<0.05). The rate of smoker in group A was significantly higher than that of the other groups (all P<0.05). The proportions of ulcer at stomach and single ulcer of group A were significant higher than that of group B (P<0.05).There were no significant difference among the four groups in sizes of ulcer (P>0.05). Conclusion: Non-Hp and non-NSAID peptic ulcer disease has an important proportion among peptic ulcer disease, the patients are old and the tenderness was common. The proportions of fudus of stomach and single ulcer are significant higher than that of the group of Hp positive only. Smoking is one of the possible causes of non-Hp and non-NSAID peptic ulcer disease.
2015 Vol. 45 (4): 286- [Abstract] ( 684 ) HTML (1 KB)  PDF (1281 KB)  ( 892 )
290 The clinicopathological and diagnostic analystic in 49 cases cervical adenocarcinom
JIN Xinhong1, WENG Ke2, WANG Shanshan3, HUANG Bifei4.
Objective: To investigate the clinical and pathological features of cervical adenocarcinoma and analyze the relevant factors which leading to misdiagnosis and missed diagnosis, aimed at early diagnosis and affirm the histological type. Methods: Clinical and pathological data of 49 cases of cervical adenocarcinoma patients, comprehensive combing the treatment and diagnosis process of all cases were retrespefively analyzed. Results: The cervical adenocarcinoma percentage of cervical cancer was 20.47% and 10.11% in 2011～2013 and 2005～2007. The main clinical symptoms of cervical adenocarcinoma were vaginal bleeding, fluid flow and increased vaginal discharge. Liquid-based cytology false negative rate was 28.57% (18/49). High-risk HPV positive rate was 93.88% (46/49). The first biopsy-confirmed cases were only 21 cases (46.94%). The pathology grossly type was mainly about nodular polypoid or endophyte, but that there were 11 cases (22.45%) was cervicitis or cervical smooth performance. The mucinous adenocarcinoma was the most to see of histology. Immunohistochemical CEA, P16 and P53 were positive. Conclusion: The meyority of clinicd syrptoms of the cervical adenocarcinama was non-specific. Utilization ofliquid-based cytology, high-risk HPV testing, colposcopy, cervical cone biopsy and immunohistochemical examination and other means to effectively master a variety of diagnostic genotyping points is the key to reducing misdiagnosis and miss diagnosis. Suggested that cervical conization as a diagnostic method for the micro-deviation adenocarcinoma, fluff tubular adenocarcinoma, cervical adenocarcinoma and early invasive adenocarcinoma.
2015 Vol. 45 (4): 290- [Abstract] ( 896 ) HTML (1 KB)  PDF (4090 KB)  ( 1911 )
295 The relationship between basal ganglia calcification and serum homocysteine, uric acid and calcium in cerebral infarction patients
HU Yazhuo, LI Ning, ZHAO Qian, WANG Li, HAN Cuijuan, SANG Chunni.
Objective: To investigate the relationship between basal ganglia calcification and serum homocysteine, uric acid and serum total calcium in cerebral infarction patients. Methods: Nigty-nine patients with cerebral infarction were divided into basal ganglia calcification group and without-basal ganglia calcification group, levels of serum homocysteine and uric acid between 2 groups were compared, and numerical values of serum total calcium and alkaline phosphatase between 2 groups were compared. Results: Serum homocysteine level and uric acid level in basal ganglia calcification group were higher than that in without-basal ganglia calcification group in patients with cerebral infarction, 2 groups were statistically significant difference (P<0.05). There was no significant difference between 2 groups of total serum calcium values (P>0.05), but the level of alkaline phosphatase was lower in basal ganglia calcification group than that in without-basal ganglia calcification group, the difference was statistically significant (P<0.05).Conclusion: Blood homocysteine, uric acid are two risk factors for disease associated with basal ganglia calcification, alkalin phosphatase is involved in the formation of basal ganglia calcification.
2015 Vol. 45 (4): 295- [Abstract] ( 620 ) HTML (1 KB)  PDF (12735 KB)  ( 614 )
298 Analysis of the hysteroscopic investigation in 341 cases with recurrent miscarriage
CHEN Zhiwen, SHI Zhengzheng, ZENG Xiaobo, LIN Ya, WU Xueqing.
Objective: To discuss the appropriate time-window for diagnostic hysteroscopy in patients with recurrent miscarriage. Methods: A retrospective study on the clinical and hysteroscopic investigation of 341 cases with recurrent miscarriage was conducted. According to the number of miscarriages (2, 3, and 4 or more consecutive miscarriages), patients were divided into three groups, and all received diagnostic hysteroscopy for uterine anomalies. Congenital (septate uterus/subseptum, arcuate uterus, unicornuate uterus, uterine hypoplasia) and acquired uterine abnormalities (intrauterine adhesions, polyp and submucous myoma) were recorded. The hysteroscopic results were compared between the groups. Results: No anomalies, congenital and acquired abnormalities in group 1, 2 and 3 were: 101 (62.7%), 42 (26.1%), 18 (11.2%); 61 (51.7%), 35 (29.7%), 22 (18.6%); 29 (46.8%), 18 (29%), 15 (24.2%). No significant differences were found in the three groups (P=0.161). Conclusion:
Diagnostic hysteroscopy should be carried out after 2 consecutive miscarriages, in order to determine congenital and acquired uterine anomalies, finally improve pregnancy outcome. Especially in those older, anxious population who cannot venture to lose another pregnancy.
2015 Vol. 45 (4): 298- [Abstract] ( 569 ) HTML (1 KB)  PDF (2279 KB)  ( 966 )
304 Different optional time leading to complication and conversion to laparotomy by laparoscopic cholecystecto my in acute calculous cholecystitis: a Meta-analysis
WU Xiaoying, WU Yuewu, WU Qi, ZHAN Weihua, SHI Jianfeng, CHEN Yiqi.
Objective: To evaluate the effects of different optional time leading to complication and conversion to laparotomy by laparoscopic cholecystectomy in acute calculous cholecystitis. Methods: Search all of domestic and international research literatures on different optional time by laparoscopic cholecystectomy about the Chinese from 1991 to 2013. The random effects model was selected for conducting the meta-analysis, to calculate the OR and 95%CI of different optional time leading to complication and conversion to laparotomy. Results: Overall 23 articles were eligible and were included into the meta-analysis, There was significant statistical difference in complication (OR=0.47, 95%CI=0.35~0.62, P<0.001) and conversion to laparotomy (OR=0.27, 95%CI=0.20~0.37, P<0.001) between the <72 h group and >72 h group. Conclusion: Meta-analysis shows that in the laparoscopic cholecystectomy progress, the surgical outcome is best within 72 h, which can reduce the complication and decline the conversion.
2015 Vol. 45 (4): 304- [Abstract] ( 613 ) HTML (1 KB)  PDF (6409 KB)  ( 959 )
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