温州医科大学学报
 
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2015 Vol. 45, No. 3
Published: 2015-03-25
 
 
157 LncRNAs expression profiles of lung squamous cell carcinoma revealed by microarray and validation
PAN Qinshi, CHEN Jian, XU Gang, DING Hongyan, HUANG Kate, WANG Yumin.
Objective: To analyze lncRNA expression profiles in lung squamous cell carcinoma and prelimiary validate. Methods: LncRNAs and mRNAs expression profiles in lung squamous cell carcinoma samples compared with NT samples were studied by high-throughput microarray. The interesting candidate lncRNAs were verified by real-time quantitative PCR. Results: Using abundant and varied probes, 30 586 lncRNAs and 26 109 coding transcripts were able to assess in our microarray. From the data we found there were thousands of lncRNAs and mRNAs that differentially expressed ($2 fold-change) and 1 741 lncRNAs and 1 716 mRNAs differentially expressed in lung squamous cell carcinoma samples compared with NT. P53, cell cycle, streptococcal infection, cell adhesion molecules signaling pathways may be involved in the pathogenesis of the lung squamous cell carcinoma. We also found, via quantitative PCR, that 10 lncRNAs were aberrantly expressed in lung squamous cell carcinoma compared with matched histologically normal lung tissues. Among these, KRT16P2 and BC016831 were the most aberrantly expressed lncRNAs, as estimated by quantitative PCR in 40 pairs of lung squamous cell carcinoma and NT samples. Conclusion: The results reveal lncRNAs expression patterns in lung squamous cell carcinoma by microarray. These lncRNAs and some pathways may play a key role in tumor development and occurrence.
2015 Vol. 45 (3): 157- [Abstract] ( 715 ) HTML (1 KB)  PDF (8722 KB)  ( 681 )
162 Adjusting and controlling effect of microRNA-494 on extracellular matrix of secretion cells in renal interstitial fibroblast
LIN Haixia, SU Zhen, SHU Danhua, HE Limei.
Objective: To explore the adjusting and controlling effect of microRNA-494 (miR-494) on extracellular matrix of the secretion cells in renal interstitial fibroblast (NRK-49F) cells after stimulation of TGF-β1. Methods: NRK-49F cells were cultured in vitro and divided into: TGF-β1 (3 ng/mL) group, miR-494 mimics (50 ng/mL) group, miR-494 inhibitor (50 ng/mL) group, co-treated with miR-494 mimics (50 ng/mL) and TGF-β1 (3 ng/mL) group, co-treated with miR-494 inhibitor (50 ng/mL) and TGF-β1 (3 ng/mL) group, blank control group. The expression of miR-494, collagen I (Col I), fibronectin (FN) mRNAs were analyzed by stem-loop real-time quantitative PCR (RT-qPCR) techniques. The expression of Col I protein was analyzed by Western Blot. Results: Compare with blank control group, the expression of miR-494 (P<0.01), Col I (P<0.05) and FN (P<0.01) mRNA were significantly increased in TGF-β1 group, the expression of Col I and FN mRNA were significantly increased in miR-494 mimics group (P<0.05), the expression of Col I (P<0.05) and FN (P<0.01) mRNAs were significantly decreased in miR-494 inhibitor group. Compared with TGF-β1 group, the expression of Col I and FN mRNAs were significantly increased in co-treated with miR-494 mimics and TGF-β1 group (P<0.05), the expression of Col I and FN mRNA were significantly decreased in co-treated with miR-494 inhibitor and TGF-β1 group (P<0.05), the expression of Col I protein was consistent with Col I mRNA (P<0.05). Conclusion: miR-494 can promote the TGF-β1 inducing secretion and deposition of extracellular matrix.
2015 Vol. 45 (3): 162- [Abstract] ( 603 ) HTML (1 KB)  PDF (6840 KB)  ( 764 )
166 Effects of electroacupuncture and constraint-induced movement therapy on the expression of GAP-43 and GFAP after middle cerebral artery occlusion in rats
FAN Liubo1, LIU Baohua2, ZHANG Xia1, LU Zhan1, TIAN Ying1, WANG Lingzhi1, MA Lizhong1.
Objective: To observe the change of electroacupuncture and CIMT on the neural function and the expression of GAP-43 and GFAP after middle cerebral artery occlusion in rats and investigate the effect of electroacupuncture and CIMT on the neural function. Methods: MCAO was established by nylon monofilament. These rats were randomly divided into 4 group: model group, CIMT group, electroacupuncture group, and electroacupuncture with CIMT group. Then each group was randomly divided into 5 subgroups by 7 d, 14 d, 21 d, 28 d,
and 35 d. Meanwhile to design a sham-operation group. The corresponding treatment was performed respectively. The evaluations included the behavior scores and Morris water maze experiment, both was to compare the recovery of neural physiological function at different time points abovementioned. Immuno-histochemical technique was used to observe the positive cells of GAP-43 and GFAP in cortical ischaemic lesions of the rats in each group at different time points. The protein expression of GAP-43 and GFAP were detected by Western blot at day after ischemia. Results: The expression of GAP-43 in gray matter of rats were obviously increased in electroacupuncture with CIMT group compared with he model group, CIMT group, and acupuncture group after 14 d, 21 d,
28 d (P<0.05), The expression of GFAP in gray matter of rats were obviously decreased in electroacupuncture with CIMT group compared with CIMT group, electroacupuncture group after 14 d, 21 d, 28 d (P<0.05), but no significant difference was found between CIMT group and acupuncture therapy group after 35 d (P>0.05). Conclusion:
Electroacupuncture combined with CIMT can improve neurological function of focal cerebral ischemia rats, which may be associated with the increase of GAP-43 and descrease of GFAP in peri-infarct regions.
2015 Vol. 45 (3): 166- [Abstract] ( 643 ) HTML (1 KB)  PDF (6139 KB)  ( 715 )
171 Effect of hepatitis B e antigen on the cytokine secretions and PI3K-Akt signaling pathway of mouse bone marrow-derived dendritic cells
WU Lecan1, WU Jinming1, LAN Songsong1, LIN Xianfan1, WANG Xiuyan1, ZHANG Teng2, HUANG Zhiming1, WU Jiansheng1.
Objective: To investigate the effect of HBeAg on cytokine secretions and PI3K-Akt signaling pathway of mouse bone marrow-derived dendritic cells. Methods: The murine bone marrow cells were cultured and induced in vitro into DCs. Then the DCs were purified by murine CD11c microbeads and were matured overnight with LPS (100 ng/mL) to generate mature DCs. All the DCs were classified into three groups randomly, the control group, the OVA group and the HBeAg group. The secretions of IL-12p70 and IL-10 in DCs of all groups and the ability of three groups to stimulate allogenic T cell proliferation were detected by the enzyme-linked immunosorbent assay and the Mixed Lymphocyte Reaction, respectively. Then the western blot was used to detect the phosphorylation of Akt in three DCs groups. Results: After the HBeAg intervention, the expression of IL-12p70 in DCs and the stimulating T cell proliferation ability of DCs were significantly decreased compared to the other two groups (P<0.05), whereas the secretion of IL-10 and the phosphorylation of Akt in DCs were significantly increased (P<0.05). Conclusion: HBeAg may exploit PI3K-Akt signaling pathway as a strategy to suppress the immune response of DCs by inhibiting the secretion of IL-12 and increasing the secretion of IL-10.
2015 Vol. 45 (3): 171- [Abstract] ( 630 ) HTML (1 KB)  PDF (8055 KB)  ( 665 )
176 The therapeutic effect of curcumin derivatives L6H4 on rats with abnormalities of glucose and lipid metabolism and its mechanism
CHEN Simei1, ZHANG Senkai2, LIU Wangwang3, XU Mengfei3, CHEN Sanmei4, CHEN Guorong3, CHEN Xiaofei1.

Objective: To observe the effect of curcumin derivatives L6H4 on rats with abnormalities of glucose and lipid metabolism, and preliminarily study the mechanism of it. Methods: Twenty-four male SD rats were randomly allocated into three groups: normal control group (NC group, n=8), high fatty group (HF group, n=8), and fatty treatment group (FT group, n=8). To fed the rats in HF group and FT group with high fat and surgar diet for 12 weeks, from the 5th week with drug treatment for 8 weeks, at the end of 12th week, the following indexes were measured respectively in each group: serum biochemical indexes, liver and skeletal muscle triglycerides, and related indicators of sugar and lipid matabolism. Observing the liver pathological changes at the same time. Results: ①Compared HF group with NC group: weight gain (P<0.05), liver weight, fasting blood glucose (FBG), fasting insulin (FINS), insulin resistance index (HOME-IR), serum triglycerides, liver triglyceride, skeletal muscle triglycerides, serum ALT, FFA, high-sensitivity c-reactive protein (hs-CRP) and interleukin 6 increased (P<0.01), adiponectin and leptin decreased (P<0.01, P<0.05, respectively). ②Compared the FT group with HF group: weight and ALT loss (P<0.01), liver weight and liver index had no obvious difference, FBG decreased (P<0.01), FINS (P>0.05), the HOME-IR decreased (P<0.01), serum triglycerides, liver triglycerides and skeletal muscle triglyceride were reduced (P<0.01, P<0.01, and P<0.05, respectively), FFA and hs-CRP reduced (P<0.01), fibroblast growth factor 21 (FGF-21) increased (P<0.01), adiponectin, interleukin 6 increased with P<0.01 and P<0.05, respectively. ③Inflammatory injury in hepatic tissue of HF groups was slight, while fatty degeneration in hepatic tissue and skeletal muscle were obvious, the condition was significantly reduced in the FT group. Conclusion: Curcumin derivatives L6H4 has the effect of controlling weight, protecting the liver function and improving the condition of insulin resistance on high fat and sugar-fed rats.
2015 Vol. 45 (3): 176- [Abstract] ( 603 ) HTML (1 KB)  PDF (11717 KB)  ( 637 )
180 Mechanism of risk factors alert and systematic inflammation response in children with obstructive sleep apnea and hypopnea syndrome
SU Miaoshang1, YU Chenyi1, ZHANG Yuanbo2, ZHOU Yangyang2, XU Li2, CAI Xiaohong1.

Objective: To investigate the risk factors alert mechanism in children with obstructive sleep apnea and hypopnea syndrome (OSAHS), and to determine the correlation of arousal and systematic inflammation response. Methods: Two hundred and eighty-five cases of snoring children aged 6-11 years old were included, sleep questionnaires were completed by their parents. All the children performed the whole night polysommongraphy (PSG), the total sleep time (TST), sleep efficiency, sleep structure, oxygen desaturation, and various respiratory disorder index were assessed by physician. Thirty children at high risk and low risk for obstructive sleep apnea syndrome (OSAHS) were randomly selected to detect the blood routine, C reactive protein (CRP) and interleukin-1β (IL-1β) levels. Results: Fifty-six cases (19.6%) of snoring children were diagnosed OSAHS by PSG. Logistic regression analysis showed that tonsil and adenoid hypertrophy, habitual snoring, obesity, mouth breathing, enuresis, hyperactivity and irascibility were the main risk factors. PSG study showed that TST, sleep efficiency, deep sleep (stage III and IV) and REM sleep time decreased, light sleep (stage I and II), arousal index (AI), OAI, AHI and RDI increased in OSAHS children. IL-1β and CRP levels increased in OSAHS children. Pearson correlation analysis showed there was positive correlation between AI and RDI (r=7.56, P<0.01). Furthermore, AI correlated with the level of IL-1β (r=6.85, P<0.01). Conclusion: Tonsil and adenoid hypertrophy, habitual snoring, obesity, mouth breathing, enuresis, hyperactivity and irascibility are the main risk factors of OSAHS children. AI increases in accord with RDI and this phenomenon may correlate with systematic mild inflamation reponse.
2015 Vol. 45 (3): 180- [Abstract] ( 715 ) HTML (1 KB)  PDF (5852 KB)  ( 698 )
185 Effects of lycopene on bone mineral density and RANKL/OPG in chronic hypoxia modal mice
ZHU Zaisheng1, DAI Shuang2, ZHENG Jingyu2, HUANG Kate2, WU Ling2, DU Xiaohong1, LI Jianmin2.
Objective: To investigate the effects of lycopene on bone mineral density (BMD) and RANKL/OPG in chronic hypoxia (CH) modal mice. Methods: Twenty-four C57BL/6 male mice aged 8 weeks were randomly divided into three groups: Control group (C group, n=8) and CH group (CH group, n=8) and lycopene group (LCP group, n=8). Mice in CH and LCP were exposed in hypoxic environment, 8 h/day, 6 d/week while the C group lived in normal environment. At the end of 8 weeks, the serum levels of OC and TRACP-5b were measured by ELISA. The content of malondiadehyde (MDA) and the activity of superoxide dismutase (SOD) in shin-bone were analyzed using hydroxylamine assay and TBA colorimetry. The BMD of right femurs were detected by Hologic QDR-4500 bone densitometer. Histomorphology of bone tissue was observed with H.E stainin under light microscopy. The expressions of OPG and RANKL in bone tissue were detected with immunohistochemical technique and RT-qPCR respectively. Results: Firstly, Histologically, the volume of bones trabecular were decreased and the bones trabecular distribution were sparse, thinner and fracture in bone marrow in CH group. In comparison with the C group mice, BMD and serum OC level and the activities of SOD in bone (P<0.01) were decreased significantly in CH group mice, while the serum TRACP-5b level, MDA level in bone, the expression of RANKL protein and RANKL mRNA in bone and RANKL mRNA/OPG mRNA (P<0.01) were increased significantly. Secondly, LCP could partially improve the histomorphometric parameters in mice with CH. The serum TRACP-5b level, MDA level in bone, the expression of RANKL protein and mRNA in bone and RANKL mRNA/OPG mRNA in LCP group mice were lower significantly than the levels in HC group mice, while BMD and serum OC level and the activities of SOD in bone were higher (P<0.01 or P<0.05). Conclusion: Lycopene can reduce oxidative stress, regulate RANKL mRNA/OPG mRNA, decrease bone resorption and improve BMD in CH mice.
2015 Vol. 45 (3): 185- [Abstract] ( 572 ) HTML (1 KB)  PDF (18304 KB)  ( 717 )
190 Change of serum adiponectin, inflammation and oxidative stress in end-stage renal disease patients, influence of dialysis and its mechanisms
CHEN Huile1, XU Changlong2, XU Xiaojie3, ZHOU Zhihong1.
Objective: To explore change of the serum adiponectin (ADPN) levels, inflammation factor and oxidative stress in patients with end-stage renal disease (ESRD), influence of maintenance dialysis treatment and its mechanisms. Methods: Seventy-eight cases of patients with ESRD were selected and divided into non dialysis group (26 cases), hemodialysis group (27 cases) and peritoneal dialysis group (25 cases), another health check-up 30 cases were chosen as control group. By the methods of enzyme-linked immunosorbent assay (ELISA) serum adiponectin (ADPN), high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were detectd, the xanthine oxidase (XO) and myeloperoxidase (MPO) activity were detected with colorimetric method, superoxide dismutase (SOD) activity was detected with xanthine oxidase method, melondialdehyde (MDA) content was detected with glucosinolates barbituric acid method. Results: Three groups of patients with ESRD ADPN, hs-CRP, TNF-α, IL-6, XO, MPO and MDA were significantly higher than that of control group, and SOD were significantly lower than that of the control group, the difference was statistically significant (all P<0.01). Two dialysis groups, respectively, compared with non dialysis group, ADPN had no statistical difference (all P>0.05), and their hs-CRP, TNF-α, IL-6, XO, MPO and MDA were significantly higher than that of non dialysis group (P<0.05 or P<0.01), SOD were significantly lower than that of the non dialysis group (all P<0.01). Correlation analysis showed that ADPN with hs-CRP, TNF-α, IL-6, XO, MPO and MDA was significantly positive correlation (r=0.584, r=0.517, r=0.461, r=0.477, r=0.563, r=0.492, respectively, all P<0.01), and SOD was significantly negative correlation (r=-0.508, P<0.01). Conclusion: A significant rise in ESRD patients serum ADPN level, the main reason may be a decline in kidney function, inflammation and oxidative stress state mutual induction, synergy. Hemodialysis and peritoneal dialysis can not reduce the ADPN, while inflammation and oxidative stress aggravate.
2015 Vol. 45 (3): 190- [Abstract] ( 650 ) HTML (1 KB)  PDF (5023 KB)  ( 736 )
194 The effect of diabetes on inflammatory response and pulmonary function in patients undergoing limb ischemia-reperfusion
WANG Liangrong, JIN Lida, LIN Lina, CHEN Lei, XIONG Xiangqing.
Objective: To investigate the effect of type 2 diabetes on inflammatory response and pulmonary function in patients undergoing limb ischemia-reperfusion induced by tourniquet, and to study its relationships with blood glucose control level and disease duration. Methods: Eight-five patients scheduled for unilateral lower extremity surgery were enrolled and divided into Diabetes (n=55) and Control (n=30) Group, Diabetes Group was allocated into several sub-groups according to blood glucose control level and disease duration, respectively. The patients received epidural anesthesia and radial arteries catheterization, and the tourniquet duration lasted for 1-1.5 h. The arterial blood gases and serum Tumor Necrosis Factor-α (TNF-α) and interleukine-6 (IL-6) were measured immediately before tourniquet inflation, 2 h, 6 h and 24 h after tourniquet deflation. Results: Serum levels of TNF-α and IL-6 were significantly elevated at 2 h, 6 h and 24 h after tourniquet deflation, and the values of alveolar-arterial oxygen tension difference (PA-aDO2) and respiratory index (RI) were increased were increased at 2 h and 6 h after tourniquet deflation in both two groups (P<0.05). As compared to Control Group, the serum levels of TNF-α and IL-6 were higher, and PA-aDO2 and RI values were larger in Diabetes Group at 6h after tourniquet deflation (P<0.05), which were closely related to blood glucose control level and disease duration.
Conclusion: Type 2 diabetes aggravates the inflammatory mediators release and pulmonary gas exchange function, which relates to blood glucose control level and disease duration.
2015 Vol. 45 (3): 194- [Abstract] ( 592 ) HTML (1 KB)  PDF (3799 KB)  ( 775 )
197 The correlation between endometriosis and placenta previa in assisted reproductive technology
FU Xiujuan, ZHOU Yong, JIN Hangmei.
Objective: To investigate placenta previa associated with endometriosis in assisted reproductive technology. Methods: Consecutive 2 060 pregnancies conceived by assisted reproductive technology were recruited between 2008 and 2012 in Women’s Hospital, School of Medicine, Zhejiang University. The risk of placenta previa after assisted fertilization was analyzed. Relation of nine variables was examined, i.e. maternal age, BMI, gravidity, parity, previous abortion, previous cesarean delivery, endometriosis, ovulatory disorder, and male infertility, to placenta previa by logistic regression analysis. The correlation between endometriosis severity with placenta previa was analyzed by the nonparametric test of Spearman correlation analysis. Results: In 110 women with placenta previa, there were 69 cases complicated with endometriosis. We found that endometriosis (odds ratio=19.7; 95% CI=0.5-300.6) were significantly associated with placenta previa. Endometriosis was more serious more prone to placenta previa (the Kendall correlation coefficient is 0.274, Spearman correlation coefficient is 0.281, all P<0.001). Conclusion: Our findings suggest that endometriosis is associated with an increased risk of placenta previa in assisted reproductive technology. The higher degree of endometriosis, the more possible of placenta previa.
2015 Vol. 45 (3): 197- [Abstract] ( 659 ) HTML (1 KB)  PDF (4679 KB)  ( 737 )
201 The study on intelligence structure of attention-deficit/hyperactivity disorder in children
CHEN Yili, ZHANG Ying, ZHOU Yonghai, LIU Peining
Objective: To study the intelligence level, intelligence structure and its balance of different attention-deficit/hyperactivity disorder in children, offer advice for clinical diagnosis and treatment. Methods: From January 2008 to Septmber 2011, 215 inattentive or hyperactivity children considered by their parents or teachers were compared. All children received DSM-IV diagnosis. Determine the intelligence in 80 ADHD group and in 30 normal grout using Wechsler Intelligence Scale for Children (WISC-CR) and make a comparison of the results between two groups. Results: The IQ of children with ADHD was normal or sub-normal. Intelligence level, full intelligence quotient (FIQ), verbal intelligence quotient (VIQ) and of ADHD children were lower than those of the normal children. The difference was statistical significance (P<0.01). But there were no significant differences in performance intelligence quotient (PIQ) between the two groups. In the subtests, the four subtests scores of information, similarities, arithmetic and digit apan had statistical significance (P<0.05). The  un-balance between VIQ and PIQ incidence rate of ADHD was higher than normal (P<0.05). It suggested that the development of intelligence structure of ADHD children was imbalanced. The girls’ subscale scores of the information, picture arrangement and block design were much lower than boys’ (P<0.05). PIQ and FIQ of girls with ADHD were lower than that of boys’ (P<0.05). Conclusion: Children with ADHD having intelligence deficit are an impersonal fact. The ADHD girls’IQ is significantly lower than ADHD boys’.
2015 Vol. 45 (3): 201- [Abstract] ( 671 ) HTML (1 KB)  PDF (4605 KB)  ( 822 )
205 The applied value of combined ditection of serum YKL-40 and SCCag in cervical cancer
DONG Xiaoxia1, HUANG Lingxiao1, LV Jieqiang2, FANG Shenhui1, LIN Xiaohua1.
Objective: To explore the applied value of combined ditection of serum YKL-40 and SCCag levels in cervical cancer. Methods: Enzyme-linked Immunosorbent Assay (ELISA) technique was applied to detect serum YKL-40 and chemiluminescence method was applied to detect serum SCCag levels in patients with cervical cancer (n=53), cervical intraepithelial neoplasias (CIN, n=35) and healthy women (n=35). Results: The 90th percentile of YKL-40 values for normal control group was 93.33 ng/mL; The serum YKL-40 and SCCag levels in cervical cancer group were significantly higher than that in each of the two control groups; the serum SCCag level differed significantly between the two control groups; However, the serum YKL-40 level did not differ significantly between the two control groups. The serum SCCag level showed a significant correlation with FIGO stage, nodal status and tumer size while serum YKL-40 showed a significant correlation with FIGO stage, but did not have a significant correlation with nodal status and tumer size. With detection of serum YKL-40 and SCCag, the sensitivity was 79.20% and 77.38%, respectively; while with combined detection of serum YKL-40 and SCCag, the sensitivity could reach 94.34%. Conclusion: SermYKL-40 may be a new tumor marker in cervical cancer diagnosis, and combined detection of YKL-40 and SCCag can improve the sensitivity of early cervical cancer diagnosis, so it has important value in detection of malignant degree, evaluation for clinical stage and surgical treatment and evaluation curative effect of cervical cancer.
2015 Vol. 45 (3): 205- [Abstract] ( 664 ) HTML (1 KB)  PDF (5275 KB)  ( 751 )
208 The evaluation on clinical effect of Chaishao Liujun Decoction in the treatment of chronic kidney disease stage 3-4
SUN Yun, DONG Feixia, CHENG Jinguo.
Objective: To evaluate the clinical effect of Chaishao Liujun Decoction therapy in treating Chronic Kidney Disease (CKD) stage 3 to 4, which is based on tonifying kidney and spleen, elevating Yang and defusing Qi. Methods: Seventy-two qualified cases were collected from CKD stage outpatients or hospitalized patients of Wenzhou hospital of traditional Chinese medicine, and the cases were divided randomly into two groups and each group was composed of 36 cases. On the basis of routine treatment, the patients in treatment group were treated with Chaishao Liujun Decoction. The treatment period was 3 months. The changes of clinical symptoms,
renal function and hemoglobin level of both groups were observed. Results: There wasn’t statistical significant difference between two groups in clinical syndromes of Traditional Chinese Medicine (TCM) and renal function level before treatment. After a treatment period, the clinical symptoms of TCM in both groups could be improved and the efficacy in the treatment group was much better, the comparison had a statistical significance (P<0.05). The efficacy of improving renal function and hemoglobin level in the treatment group was better than that of the control group’s, the comparison had a statistical significance (P<0.05). The clinical therapeutic effect in the treatment group was better than that of the control group, the comparison had a statistical significance (P<0.01); Both two groups were no adverse reaction. Conclusion: The Chaishao Liujun Decoction can improve clinical symptoms, renal function and hemoglobin of patients with CKD stage 3 to 4, and delay the progression of CKD.
2015 Vol. 45 (3): 208- [Abstract] ( 671 ) HTML (1 KB)  PDF (4836 KB)  ( 838 )
212 Comparative study on the screening methods of cervical cancer
LIN Ying1, LI Wei2, ZHU Yangli2, RUAN Haibo1, LI Danjun1, ZHENG Meiyun1.
Objective: To investigate the most effective opportunistic screening method of the precancerous lesion and early cervical cancer. Methods: Pap smear, liquid-based cytologic test (LBC), human papillomavirus DNA with a second-generation hybridization assay (HC2) were performed in 856 women in First People’s Hospital of Wenling from March 2011 to May 2013. The results were evaluated by the colposcopic biopsy which was considered as gold standard. Negative colposcopy was accepted as a negative outcome. Results: The sensitivity of Pap smear, LBC, HC2 was 55.3%, 80.9%, and 87.3%. The specificity was 94.1%, 95.8% and 83.6%. The negative predictive value was 93.5%, 97.1% and 97.8%. The positive predictive value was 58.1%, 74.2% and 44%. Conclusion: LBC is an effective screening method of primary opportunistic cervical cancer screening.
2015 Vol. 45 (3): 212- [Abstract] ( 660 ) HTML (1 KB)  PDF (5126 KB)  ( 748 )
215 Comparison of therapeutic efficacy of nasogastric tube and nasointestinal tube in elderly critically ill patients
XU Weiyi.
Objective: To compare the effectiveness of use of nasogastric and nasointestinal tube in elderly critically ill patients. Methods: Eighty elderly critically ill patients required enteral nutrition were randomly divided into nasogastric tube group and nasointestinal tube group. The nutriture in each patient were observed every 5 days, and complications and 30-day mortality during stay in ICU were also compared between two groups. Results: Serum albumin in nasointestinal tube group was significantly increased compared with that in nasogastric tube group (P<0.05). However, the other nutritive indicators were not significantly different between two groups (P>0.05). The incidence of reflux and aspiration pneumonia and 30-day mortality during stay in ICU  were significantly lower than that in nasointestinal tube group (P<0.05). Conclusion: Enteral nutrition through nasointestinal tube is effective for elderly critically ill patients with high safety, and the incidence of reflux and aspiration pneumonia can be reduced to some extent.
2015 Vol. 45 (3): 215- [Abstract] ( 766 ) HTML (1 KB)  PDF (3719 KB)  ( 758 )
218 IL-10, IFN-γ for the study of the significance of detecting for children with hemophagocytic syndrome
XU Zhisheng, ZHENG Shuangli, LI Yuan, ZENG Weiwei, CHEN Min, QIAN Jiangchao.
Objective: To evaluate serum IL-6, IL-10, IL-18, IFN-γ for the value of early diagnosis and prognosis of children with hemophagocytic syndrome. Methods: By using the method of prospective study of 20 children with a diagnosis of HPS into diagnosis before treatment and after treatment, while at the same time, with 20 cases normal physical examination children as normal control group, then choose the remission of acute lymphoblastic leukemia (ALL), 18 cases and sepsis were 22 cases of serum were chosen as positive control group. IL-6, IL-10, IL-18, IFN-γ levels were determined with flow cytometry instrument flow ball technique. Four kinds of cytokines serum levels before and after treatment between different groups were compared. Results: Four kinds of cytokines levels obriously varied with HPS before and after treatment in children, 4 kinds of cytokines during the diagnosis level was significantly higher than that of the normal control group after treatment (P<0.05), children with HPS of IL-10 and level of IFN-γ obviously elevated compared with normal controls, children with acute leukemia and the cases of sepsis was significant difference (P<0.05). Conclusion: Cytokines IL-10 and IFN-γ levels in children with HPS before treatment is significantly elevated compared with the control group, then markedly reduced for treatment, so it can be used as a children’s early diagnosis and prognosis prediction of HPS clinical indicator.
2015 Vol. 45 (3): 218- [Abstract] ( 635 ) HTML (1 KB)  PDF (4453 KB)  ( 712 )
222 A curative observation of surgical crown lengthening in split teeth restoration
GAO Huiying
Objective: To explore the clinical effect of surgical crown lengthening on restoration of broken teeth. Methods: Sixty-eight broken teeth in 62 patients were selected, and were randomly divided into group A and B, 31 patients per group, 36 broken teeth in group A and 32 broken teeth in group B. Patients in group A were given gingivectomy, and patients in group B were given crown lengthening surgery. Broken end and gingival margin of broken teeth before and after surgery were compared, including plaque index (PLI), sulcus bleeding index (SBI), periodontal probing depth (PD), gomphiasis and repair effect. Results: All patients were successfully performed surgery according to plan, periodontal index after surgery were improved (P<0.05), yet there were no statistical differences on periodontal index between two group (P>0.05). After operation, teeth mobility rate in group A was 25.00%, yet there was no tooth mobility in group B, and group A’s teeth mobility rate was higher than that in group B (P<0.05). Two groups’ effective rate of teeth restoration were both 100%, and satisfaction rate were 63.89% and 81.25% respectively in group A and group B. Conclusion: It has good effect of broken teeth restoration by crown lengthening surgery, the procedure can improve and maintain a healthy repaired result. If the indication right, can control the periodontal inflammation.
2015 Vol. 45 (3): 222- [Abstract] ( 531 ) HTML (1 KB)  PDF (3917 KB)  ( 783 )
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