温州医科大学学报
 
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2014 Vol. 44, No. 9
Published: 2014-09-25
 
 
625 Theoretical study on the conformation disintegration of ionic Glycine
JIN Qin, SUN Xiaoling, LIU Siyi, LIN Jingran, CHEN Yuanyuan, WANG Yanni, WANG Chaojie.
Objective: To study the conformation disintegration of glycine in cationic, anionic, and zwitterionic states in gas phase and solvent environments with cyclohexane and water. Methods: The geometric and electronic structures, vibrational frequencies, thermodynamic functions of ionic glycine were calculated fully at the CAM-B3LYP and B3LYP theory levels using 6-311++G (2d, p) basis set. The solvent effects were dealt with the polarizable continuum model (PCM). Results: To the cationic state, there were 3, 3, and 5 conformers, and to the anionic state, only 1, 1, and 3 conformers existed in three environments, but there was only 1 zwitterionic conformer in the water environment and its energies were higher slightly than the most stable neutral conformer. Two methods got the similar properties values but the former optimized bonds distances were shorter slightly and the latter obtained energy values were a little lower. Conclusion: The conformation disintegration of ionic glycine in different environments is different. The polarity of solvent increases, then more polar conformers may be presented.
2014 Vol. 44 (9): 625- [Abstract] ( 638 ) HTML (1 KB)  PDF  (0 KB)  ( 365 )
631 The effects of inflammatory mediator and oxidative stress on bone metabolism in high fat diet-induced obesity mice and the underlying mechanism
ZHU Zaisheng1, DAI Shuang2, WU Ling2, ZHENG Jingyu2, HUANG Kate2, LI Jianmin2.
Objective: To investigate the effects of inflammatory mediator and oxidative stress on bone metabolism in high fat diet-induced obesity mice and the underlying mechanism. Methods: Sixteen C57BL/6 mice aged 5 weeks were randomly divided into two groups. High fat diet group (HFD) was fed with high fat diet while normal fat diet group (NFD) was fed with normal fat diet. At the end of 16 weeks, the visceral fat was weighed and the visceral fat/body mass ratio was assessed. The levels of TRACP-5b, OC, IL-6 and TNF-α in serum were measured by ELISA. The content of malondiadehyde (MDA) and the activity of superoxide dismutase (SOD) in shin-bone were analyzed by using hydroxylamine assay and TBA colorimetry. The bone mineral density (BMD) of right femurs were detected by Hologic QDR-4500 Bone Densitometer. The expressions of OPG and RANKL in bone tissue were detected by immunohistochemical technique and RT-qPCR respectively. Histological changes in the left femurs were studied with HE stained under light microscopy. Results: Compared to the NFD group mice, the body weight,the ratio of visceral fat/body mass and the RANKL/OPG in bone, the serum levels of TRACP5b, IL-6, TNF-α, the expressions of RANKL and the content of MDA in bone were increased in HFD group mice significantly (P<0.05). But the serum level of OC, the expressions of OPG, the activity of SOD in bone and the BMD were decreased in HFD group mice significantly (P<0.05). Histologically, the volume of bones trabecular were decreased and the bones trabecular distribution were sparse, thinner and fracture and more adipocytes infiltrated in bone marrow in HFD group. The results of correlation analysis shows that BMD was positively correlated with SOD of bone (r=0.627, P<0.01) and negatively correlated with the ratio of visceral fat/body mass (r=-0.858, P<0.01), MDA level of bone (r=-0.538, P<0.01); SOD was positively correlated with OPG mRNA (r=0.637, P<0.01) and negatively correlated with TNF-α (r=-0.673, P<0.01), IL-6 (r=-0.874, P<0.01), RANKL mRNA (r=-0.760, P<0.01) and the ratio of the RANKL/OPG in bone(r=-0.768, P<0.01), but MDA shows no correlation with TNF-α, IL-6, OPG mRNA, RANKL mRNA and the ratio of the RANKL/OPG in bone (P>0.05). Conclusion: High fat diet induced obesity can lead to the increase of bone resorption and  the pathological histology changes of osteoporosis and the BMD decline. The underlying mechanism may be associated with its upreguating of inflammatory mediator and enhancing oxidative stress in bone.
2014 Vol. 44 (9): 631- [Abstract] ( 820 ) HTML (1 KB)  PDF  (0 KB)  ( 479 )
637 Study on the effect and mechanism of ginsenoside Rg1 on survival and differentiation of implanted BMSCs in dementia model rats
WU Wei1, CHEN Xiaohong2, YANG Jingquan3, WANG Xiaotong1
Objective: To explore the effect and mechanism of ginsenoside Rg1 on survival and differentiation of implanted BMSCs in dementia model rats. Methods: Forty five male SD rats were randomly divided into the FF transected model group (model group: ambi-hippocampal fimbria-fornix transected), the bone marrow mesenchymal stem cells (BMSCs treatment group): Two weeks after the model-made, every rat received transplantation of BMSCs (10 µL, 1×106 cells) into hippocampus on both sides by Hamilton microinjector with stereotaxis, combining group (ginsenoside Rg1 combining BMSCs treatment group) received both transplantation of BMSCs and peritoneal infusion of ginsenoside Rg1 (Two weeks after the model-made, every rat received peritoneal infusion of ginsenoside Rg1 (5 mg/kg), one day a time, until a month). Survival and migration of implanted BMSCs were observed by immunohistochemistry staining. Differentiation of BMSCs was observed using immunohistochemistry double staining. In order to explore the mechanism of ginsenoside Rg1 increased the number of implanted BMSCs, RT-PCR technique was used to detect the change of NGF mRNA expression in hippocampus. Results: Six weeks after the model-made, The number of BrdU positive cells in hippocampus of combining treatment group (34.2±5.4) was more than those of simple BMSCs treatment group (10.3±4.3), difference was significant (P<0.05). The level of NGFmRNA expression in hippocampus of combining treatment group (1.13±0.21) was higher than that of BMSCs treatment group (0.75±0.12), difference was significant. Conclusion: ginsenoside Rg1 is benefit to the survival of implanted BMSCs. Ginsenoside Rg1 may facilitate the survival of implanted BMSCs by up-regulating content of NGF mRNA in hippocampus
2014 Vol. 44 (9): 637- [Abstract] ( 564 ) HTML (1 KB)  PDF  (0 KB)  ( 317 )
641 Preparation and biocompatibility of kidney acellular matrix in rats as tissue engineering scaffold
DAI Kaiyu1, YI Xudong2, ZHAO Lina1, YU Yaling1, ZHANG Jianse1,3, MEI Jin1,3
Objective: To prepare the kidney decellularized matrix bio-derived scaffold with lower antigenicity, complete protein components and good biological properties in rats and evaluate its biocompatibility in vivo. Methods: By the perfusion with chemical detergents through the abdominal aorta, the acellular scaffolds of kidney were prepared in SD rats. Then the decellularized scaffolds and native kidneys were analyzed through genomic DNA content analysis, transmission electron microscopy (TEM), HE, PAS, Massons and immunofluorescence. Acellular scaffolds were transplanted in situ to evaluate the biocompatibility. Results: After being treated by a variety of reagents, cells within kidney tissue had been largely removed. The acellular scaffold had forms of three-dimensional network structure with integrated vascular network. DNA contents of the acellular renal ECM in the experimental group were 3%, less than that in the normal group. Immunohistochemistry showed a lot of collagen fibers and no significant residual cell debris within scaffold was found. The gross and histological observation of subdermal implantation showed the inflammatory reactions were severe onset. There was an increased collagen fiber density and angiogenesis, and the transplanted acellular matrix was gradually degraded and absorbed. Conclusion: The renal scaffolds prepared in rats are acellular but have the structure of three-dimensional network. The reticular structure of the extracellular matrix is well preserved, which has good biocompatibility in vivo as tissue engineering scaffold for renal reconstruction and regeneration
2014 Vol. 44 (9): 641- [Abstract] ( 619 ) HTML (1 KB)  PDF  (0 KB)  ( 420 )
646 Expression and localization of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc
CHEN Hongliang1, KE Xiurong2, LOU Chao1, LI Youyu2, HU Chunfei2, GUO Caiyun2, CHEN Sisi2, XU Huazi1
Objective: To investigate the expression of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc. Methods: Seven nucleus pulposus specimens were obtained from 7 patients during surgery. Cultured disc cells were used for immunocytochemical localization of ERβ. mRNA was isolated from cultured human disc cells, and RT-PCR amplification of ERβ was employed to document molecular expression of this receptor. Results: Expression and localization of estrogen receptor-β were detected in all 7 specimens. A goldview-stained agarose gel electrophoresis showing gene expression of ERβ as a single band at the predicted 323 bp size. Localization was seen in the cytoplasm in all cultured disc cells with the immunocytochemistry method. Conclusion: This study demonstrates the expression of estrogen receptor-β in nucleus pulposus cells of the human intervertebral disc
2014 Vol. 44 (9): 646- [Abstract] ( 652 ) HTML (1 KB)  PDF  (0 KB)  ( 522 )
649 Efficacy and mechanism of thalidomide combined with chemotherapy in the treatment of patients with acute leukemia
ZHU Baoling1, HE Muqing1, LIN Xiaoji1, LI Baoqing2, GUO Wenjian1, PAN Xiahui1, LIN Ying1, YAO Rongxin1.
Objective: To observe the clinical effect of thalidomide combined with chemotherapy in the treatment of acute leukemia patients and its’ influence on plasma concentration of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), bone marrow microvessel density (MVD) level. Methods: Forty cases of acute leukemia patients, randomly divided into trial group and conventional group with 20 cases in each group. The other 20 healthy peoples were selected underwent physical examination as control group. The trial group and conventional group were treated with conventional chemotherapy, in the trial group additional thalidomide 100 mg/day were taken orally. Before treatment and 8 weeks after treatment, plasma were collected for the detection of VEGF, bFGF and bone marrow tissue for MVD. Results: The effective rate of the trial group and the conventional group respectively were 85% and 70%, the effective rate of the trial group was higher than that of the conventional group, but the difference wasn’t statistically significant (P>0.05). Before treatment,the level of VEGF, bFGF in plasma of the trial group and the conventional group was significantly higher than that in the healthy group (P<0.05), and there’s no significant difference between the trial group and the conventional group. After treatment, the levels of VEGF and bFGF of the trial group and the conventional group were still higher than those of the healthy group (P<0.05), but there’s no significant differences in bFGF levels before and after treatment (P>0.05), the levels of VEGF in the trial group was lower than those in the conventional group after treatment (P<0.05). Linear regression analysis indicated that the VEGF of plasma and the MVD level was negatively correlated with clinical effects (β=-0.065, t=-2.215, P<0.05; β=-0.078, t=-2.451, P<0.05), and there’s no correlation between bFGF and clinical effects. Conclusion: Thalidomide combined with chemotherapy can improve the remission rate of acute leukemia patients, the mechanism of anti-leukemia may contribute to inhibiting the plasma levels of VEGF and decreasing MVD, and antiangiogenic activity.
2014 Vol. 44 (9): 649- [Abstract] ( 811 ) HTML (1 KB)  PDF  (0 KB)  ( 538 )
653 Study on the normal perfusion of the left ventricular myocardium at resting state using 320-slice volume CT
Kong Jianguo, Xu Huazhi, Wu Enfu, Hu Hao, Jiang Xin, Wei Peiying, Zhang Hua, Zheng Yongfei
Objective: To analyze the myocardial perfusion differences among each segment of the left ventricle using 320-slice volume CT in normal adults at resting state. Methods: One hundred and thirteen cases (68 males, 45 females; mean age 54±10 years) with no stenosis in coronary arteries based on coronary CT angiography (CTA) were included in the study. The data of CTA were used for the myocardial perfusion analysis with 16-segment standard model (excluding apex). The attenuation density (AD) and transmural perfusion ratio (TPR) of each segment were respectively recorded, and were compared in the same slice or among different slices. Results: In basal, middle and apical slices, the AD and TPR of the ventricular septum were (88.09±16.67) Hu and 1.21±0.20, (89.61±15.36) Hu and 1.13±0.14, (85.34±17.09) Hu and 1.24±0.17, respectively, which were higher compared with those of the non-septal walls (P<0.001). In basal and middle slices, AD of the anteroseptal myocardium (2, 8) were (92.28±16.71) Hu, (93.69±15.30) Hu, respectively, higher than those of each remaining segment (P<0.001). The apical slice (77.59±18.14) Hu showed lower AD than basal and middle slices (P<0.001). Conclusions: In adults with normal coronary arteries, the myocardial perfusion among each segment of left ventricle is different. The septum shows significantly higher AD and TPR than the anterior, lateral and inferior walls. The apical attenuation shows relatively lower value
2014 Vol. 44 (9): 653- [Abstract] ( 628 ) HTML (1 KB)  PDF  (0 KB)  ( 507 )
657 Observation on effects of the small follicles puncture using  Gn 7-9 days in vitro fertilization cycles
CHI Hai hong, HUANG Zhaoxia, CHENG Jing, CHEN Hua, GE Hongshan, LV Jieqiang
Objective: To observe clinical outcome of the patients with excessive follicular development in controlling ovulation process treating with the small follicles puncture. Methods: Sixty-four infertile women in our center accepted IVF treatment during the period from April 2011 to April 2012 were analyzed retrospectively. All of them had excessive follicular development and had the tendency of ovarian hyperstimulation syndrome (OHSS). They were divided into two groups based on the their wills. The follicle puncture group (n=36) and control group (n=32). The general index, the laboratory and the clinical outcome of the two groups were compared. Results: There were no statistically significant differences in age, time of infertility, basal and the day of follicles puncture hormones level (P>0.05). The day of hCG serum level of estradiol (estrogen, E2) and the number of oocyte retrieved in follicle puncture group was obviously lower than that in control group (P<0.05). The rates of fertilization, cleavage, and clinical pregnancy in follicle puncture group was obviously higher than that in control group (P<0.05). The follicle puncture group had no severe ovarian hyperstimulation, but the severe OHSS occurred in 13 patients (13/32, 40.6%) of control group. Conclusion: The small follicle puncture can effectively reduce the incidence of OHSS and improve the outcome of IVF-ET cycles.
2014 Vol. 44 (9): 657- [Abstract] ( 650 ) HTML (1 KB)  PDF  (0 KB)  ( 430 )
660 Anti-flammatory and analgesic effects of ethyl acetate extract from Curcuma wenyujin
QIU Guanguan, CAI Yuan, FANG Lianglian, LIN Weiwei, DONG Jianyong.
Objective: To investigate the analgesic and anti-flammatory effects of the ethyl acetate extract of Curcuma wenyujin and explore the anti-flammatory mechanism of this effective fraction. Methods: By using hot plate test, xylene-induced mouse ear edema test and chemical irritation-induced writhing test to study the effect of analgesic and anti-flammatory. The levels of TNF-α, IL-1β, IL-6 in ear homogenate were measured by ELISA. Results: The results showed that the ethyl acetate extract of Curcuma wenyujin had the significant analgesic
effects by physical and chemical stimulated. It had obviously inhibited inflammatory edema and celiac capillary permeability, the analgesic effects and anti-flammatory was improved with increased dosages. It could also reduce the level of TNF-α. Conclusion: The ethyl acetate extract of Curcuma wenyujin have the significant anti-flammatory and analgesic effects, the mechanisms of anti-flammatory is concerned with inhibiting the level of TNF-α
2014 Vol. 44 (9): 660- [Abstract] ( 723 ) HTML (1 KB)  PDF  (0 KB)  ( 506 )
664 Effects of homocysteine on expression of IL-1β, IL-6 in gitter cells
MAO Ruiyang, WANG Chuanyi, DU Xiaohong.
Objective: To investigate the effect of homocysteine on the expression of interleukin-1β (IL-1β), interleukin-6 (IL-6) in gitter cells. Methods: The BV-2 cells were cultured in the cell culture fluid for 24 hours. HCY/DL-CY or the control solvent were added, and a cell counting kit (cck8) was used to observe the proliferation activity. Enzyme linked immunosorbent assay (ELISA) was used to evaluate the concentration of IL-1β and IL-6 in the cell culture supernate. Well, mRNA levels of IL-1β and IL-6 were analysed by Real-time Quantitative PCR Detecting System (QPCR). Results: The proliferation activity in each group were not significantly different, and the mRNA expression and protein expression of IL-1β and IL-6 in the supernatant homocysteine group was significantly changed. Conclusion: Homocysteine increases the  expression of IL-1β, IL-6 in gitter cells.
2014 Vol. 44 (9): 664- [Abstract] ( 623 ) HTML (1 KB)  PDF  (0 KB)  ( 487 )
667 The relationship between EGFR gene mutation and serum tumor markers in non-small cell lung cancer patients
PU Guimei1,3, YE Junru1, LU Yao1, CHEN Bicheng2, OUYANG Jinsheng1, LI Yuping1
Objective: To analyze the relationship between EGFR gene mutation and serum tumor markers in non-small cell lung cancer (NSCLC) patients and investigate the tumor markers that can predict the EGFR gene mutation. Methods: Retrospectively analyze of 188 NSCLC patients’ clinical characteristic in our hospital, who were detected EGFR gene mutation from October 2010 to August 2013. All the patients tested the serum tumor markers before treatment. Statistical methods were used to analyze the relationship between EGFR gene mutation and basic clinical characteristics and serum tumor markers. Results: 188 NSCLC patients were enrolled, and mutation rate was 44.7 percent. EGFR gene mutation was mainly occurred in women, non-smoking and adenocarcinoma patients (P<0.05). When the serum CEA>20 μg/L, or the serum SCCAg≤1.5 μg/L, EGFR mutation rate was significantly increased. Most importantly, EGFR mutation rate was highest when SCCAg≤1.5 μg/L and CEA>20 μg/L group, and the difference was statistically significant (P<0.05). While there was no correlation between CYFRA21-1, NSE, CA125, CA199 and EGFR gene mutation (P>0.05). Conclusion: For the patients whose EGFR gene mutation can not be tested, we can chose those whose serum SCCAg≤1.5 μg/L and CEA>20 μg/L, and according to the patients’ clinical characteristic, with EGFR-TKIs treatment.
2014 Vol. 44 (9): 667- [Abstract] ( 743 ) HTML (1 KB)  PDF  (0 KB)  ( 538 )
672 Comparison of curative effect of fiber post associated with casting pure titanium ring and casting pure titanium post on reparation of subgingival residual root
HUANG Lei, WANG Shu.
Objective: To explore the curative effect of the fiber post associated with casting pure titanium ring and casting pure titanium post on the reparation of subgingival residual root. Methods: Fifty teeth were selected by the reparation of subgingival residual root after root canal therapy in our department during 2008-2011. Twenty-five teeth were received the reparation by casting pure titanium ring and fiber post and all-ceramic crown. Other 25 teeth were received the reparation by casting pure titanium post and all-ceramic crown. Then observed for 2 years. The effect was estimated from 3 aspects; the stability of the core; root fracture or not; and the gum around the prostheis. Results: Within the 25 cases treated with casting pure titanium post core crown, one happens root fracture, four cores shed from root, and zero happen gingival discolor. But other 25 cases work well and have no adverse sign. There was significant difference in the root fracture and shed from root (P<0.05). Conclusion: Using pure titanium ring and fiber post have obvious advantages than using pure titanium post in combining with the root tissue and stress distribution of root and the best visual impression
2014 Vol. 44 (9): 672- [Abstract] ( 593 ) HTML (1 KB)  PDF  (0 KB)  ( 410 )
675 Prevention of contrast-induced nephropathy with trimetazidine in patients with renal insufficiency
CHEN Yan, HU Yadi, YE Xiaorong.
Objective: To evaluate the efficacy of trimetazidine (TMZ) in the prevention of contrast-induced nephropathy (CIN) in patients with renal insufficiency. Methods: We conducted a prospective, randomized, controlled trial, 124 patients undergoing coronary angiography/angioplasty were involved, whose stable serum creatinine levels were at least 103 μoml/L. They were divided into TMZ group (n=64) and control group (n=60) randomly, TMZ group received TMZ (20 mg thrice daily) orally for 7 d starting 48 h before the procedure. All patients were given routine hydration treatment before and after the procedure. Serum creatinine (Scr) levels were measured before the procedure, 24 h, 48 h and 7 d after the procedure. Results: ①Scr levels had no statistical significance in the two groups 24 h after the procedure (P>0.05). ②Scr levels significantly decreased after 48 h and 7 d in the TMZ group (P<0.05). ③CIN developed in 3.1% of patients in the TMZ group and in 16.7% of patients in the control group (P<0.05). Conclusion: TMZ along with isotonic saline hydration is more effective than isotonic saline alone in reducing the risk of CIN in patients with renal insufficiency
2014 Vol. 44 (9): 675- [Abstract] ( 686 ) HTML (1 KB)  PDF  (0 KB)  ( 396 )
678 Clinical research of measurement of anatomical proximal femor using X ray in equalizing limb length in total hip arthroplasty
CHEN Chengwang, PAN Xiaoyun, XUE Enxing, WEN Hong
Objective: To explore the clinical meaning of measurement of anatomical proximal femor in equalizing limb length in total hip arthroplasty. Methods: From Feburary 2011 to December 2012, 120 patients including 65 males and 55 females underwent unilateral THA. The clinical measurement and radiographic examination were accomplished. The discrepancy of limb length was measured using PACS system in computer, and at the meantime, from the other hip, the angle of the anatomical axis of femur and the line linked the center of femoral head and the top of great trochant was measured. Based on these results, the surgical protocols were designed, the type of hip prosthesis was chosen, and the neck length of femoral prothesis, and the position of osteotomy were estimated. By the proper wearing of the acetabula, the best rotation point was found out. According to the results of radiographic and other examination, the neck length was readjusted after the insertion of the prosthesis so as to achieve intended leg length equalization. The discrepancy of the leg length was measured and evaluated after operation. Results: The incision healed by first intention in all patients. The leg length equalization was got in 108 patients (<0.5 cm, 68 cases; 0.5~1.0 cm, 40 cases), and only 12 limbs had been lengthened more than 1.0 cm. One hundred and two patients were followed up for 15.1 months on average (5~27 months). Setting the results against those before surgery (46.2±7.2), the mean Harris score was 86.5±6.9 points after surgery. Conclusion: With the measurement of the anatomical proximal femor using X ray accompanying other methods before and during operation, the problem of unequal leg length can be well solved in total hip arthroplasty.
2014 Vol. 44 (9): 678- [Abstract] ( 801 ) HTML (1 KB)  PDF  (0 KB)  ( 529 )
682 Effects of maternal fever caused by infection during pregnancy on the prognosis of perineonate
WU Liqun, GAO Jie, CAI Pingsheng, QI Huijiao

Objective: To investigate the correlation between maternal fever caused by infection during pregnancy and the prognosis of perineonate. Methods: The clinical data of 92 cases in our hospital with maternal fever caused by infection during pregnancy were analyzed retrospectively. Results: The chief causes of maternal fever caused by infection during pregnancy were upper respiratory tract infection and premature rupture of membranes. The 92 cases were divided into group I, including 41 cases of highest body temperature exceed 38.5 ℃
and group II, including 51 cases of highest body temperature lower than 38.5 ℃. Meanwhile, 52 cases with the time of termination of pregnancy less than 12 h and 40 cases with the time of termination of pregnancy longer than 12 h were assigned into group A and group B respectively. The findings showed that the incidences of intra-uterine asphyxia, neonatal asphyxia, neonatal hypoxic, ischemic encephalopathy and neonatal septicemia in group I were significantly higher than those in group II (P<0.05). The incidences of intra-uterine asphyxia, neonatal asphyxia, neonatal pneumonia, neonatal hypoxic, ischemic encephalopathy and neonatal septicemia in group A were significantly lower than those in group B (P<0.05). The plasma concentrations of tumor necrosis factor-α in two patients were raised apparently, of whom neonatal septicemia appeared. Conclusion: Major causes of maternal fever caused by infection during pregnancy are upper respiratory tract infection and premature rupture of membranes. The severe level of fever and the occasion of termination of pregnancy have considerable influence to prognosis of perineonate.
2014 Vol. 44 (9): 682- [Abstract] ( 630 ) HTML (1 KB)  PDF  (0 KB)  ( 394 )
685 A clinical analysis of 108 cases of type A influenza virus pneumonia in children in Wenzhou area
ZHANG Lele, ZHANG Hailin, CHEN Xiaofang, SU Miaoshang, HU Xiaoguang, LUO Yunchun
Objective: To investigate the clinical features of Wenzhou area children hospitalized for influenza A virus associated pneumonia. Methods: Retrospective analysis 108 cases virus antigen detection in our hospital by directed immunofluorescence confirmed influenza A virus related clinical data of children with pneumonia. Results: Under 3 years old to see more, accounted for 70.3%. The 2010 influenza A virus associated pneumonia infection peak for the autumn, 2011 for the winter, spring was in 2012. Twenty four cases of children in the coexistence of basic diseases, the most common congenital heart disease. Clinical with fever and respiratory symptoms as the main performance, combined with other pathogenic infections in 26 cases, the merger mycoplasma pneumoniae and streptococcus pneumoniae infection. After the treatment, 96 cases were cured, 11 cases improved and discharged, 1 cases of death, the average hospital stay was 8.5 days. Conclusion: Influenza A virus associated pneumonia in children under 3 years old for high-risk groups, infection of peak time and season is not fixed, with fever and respiratory symptoms as the main performance, easily combined with other pathogenic infections, with basis diseases complicated with severe complications.
2014 Vol. 44 (9): 685-688 [Abstract] ( 707 ) HTML (1 KB)  PDF  (0 KB)  ( 540 )
704 The role of dual time point PET/CT imaging in diagnosis of solitary pulmonary nodule with low metabolism
LIN Jie, TANG Kun, YIN Weiwei, ZHENG Xiangwu, CHEN Yangzong, LIN Xinshi, DAI Yunfei, BAO Chunchun, ZHANG Xiuxing, WANG Zhiqiang, ZHAO Liang.
Objective: To evaluate the role of dual time point 18F-FDG PET/CT imaging in diagnosis of solitary pulmonary nodule (SPN) with low metabolism. Methods: During August 2011 to June 2013, forty-five patients confirmed with SPN and SUVmax≤2.5 at early PET/CT imaging were retrospectively analysed (male 27, female 18, mean age 59.71±11.42 years). All patients were performed with early and delayed PET/CT imaging at 60 min and 120 min after intravenous injection respectively. The eraly and delayed maximum standardized uptake value (SUVmax) of PET/CT imaging were measured and the change of SUVmax (△SUVmax) was calculated. The sensitivity, specificity and accuracy were calculated using the △SUVmax≥0 as diagnostic positive standard, and the receiver operating characteristic (ROC) curve was used to evaluate the diagnostic vale of △SUVmax for low metabolic SPN. Results: The differences in average SUVmax of early and delayed imaging between benign and malignant nodules had no statisticly significance (t=0.193, P=0.848, t=1.452, P=0.154). There was significant difference in mean △SUVmax between malignant and benign lesions (t=2.738, P=0.009). The sensitivity, specificity, accuracy, positive predictive value and negative predictive value were 87.88%, 66.67%, 82.22%, 87.88% and 66.67% respectively with △SUVmax≥0 as diagnostic positive standard. The area under ROC curve was 0.861 according to the △SUVmax. Conclusion: The dual time point PET/CT imaging is useful for differentiating solitary pulmonary nodules with early SUVmax≤2.5 and without specific morphological characteristics.
2014 Vol. 44 (9): 704- [Abstract] ( 708 ) HTML (1 KB)  PDF  (0 KB)  ( 508 )
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