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2014 Vol. 44, No. 4 Published: 2014-04-25   235 Influencing factors of good pathological tumor regression grades after neoadjuvant therapy in locally advanced rectal cancer ZUO Zhigui1, WANG Hao2, GAO Xianhua2, YU Zhiqi2, DOU Weilong2, FU Chuangang2, SONG Huayu1, LI Ji1. Objective: To analyze the influencing factors of good pathological tumor regression grades after neoadjuvant chemoradiotherapy (CRT) in locally advanced rectal cancer (LARC) and evaluate the value of standardized concurrent preoperative CRT in improving the clinical efficacy in LARC. Methods: A total of 122 patients with LARC receiving neoadjuvant CRT between January 2002 and December 2009 were analyized retrospectively. The tumor regression grades (TRG) were detected by HE staining. Logistic regression analysis was used to evaluate the influencing factors associated with clinical efficacy after neoadjuvant CRT in LARC. Results: Fifty-two patients reached good TRG, including 11 patients with pathological complete response, 70 patients were poorly responsive to radiation. The good response rate after long-course radiation therapy (RT) was significantly higher than that of patients who underwent short/medium course RT (60% vs 30.56%, P=0.005), the good response rate with concurrent preoperative CRT was significantly higher than that of patients with preoperative RT alone (54.05% vs 25%, P=0.002), the good response rate after RT dose>4 000 cGy was significantly higher than that of patients underwent RT dose≤4 000 cGy (60.42% vs 31.08%, P=0.001). Logistic regression analysis showed the concurrent preoperaticve CRT and long-course RT were independently associated with the clinical efficacy in LARC after neoadjuvant CRT. The rate of patients received concurrent preoperative CRT, long-course RT, RT dose>4 000 cGy and the interval between neoadjuvant CRT and surgery≥6 weeks between January 2006 and December 2009 was significantly higher than that of patients between January 2002 and  December 2005 (71.43% vs 51.51%, 57.14% vs 27.27%, 48.21% vs 30.30%, 50.0% vs 28.79%). Conclusion:Concurrent preoperative CRT and long-course RT provide higher good response rate, standardized preoperative concurrent CRT may improve the clinical efficacy in patients with LACR.  2014 Vol. 44 (4): 235-240 [Abstract] ( 754 ) HTML (1 KB)  PDF (895 KB)  ( 687 ) 241 Effect of siRNA on proliferation of glioma cell line U87 by targeting fibroblast activation protein alpha CHEN Yuan, WANG Wei, TU Ming, WU Zhebao, ZHENG Weiming. Objective: To explore the effect of small interfering RNA (siRNA) -mediated fibroblast activation protein alpha knock-down on proliferation of glioma cell line U87. Methods: The experiment is divided into Blank, NC and siRNA. Negative control or siRNA were transfected into U87cells by Lipofectamine 2000. After transfection, protein level of target gene FAP-α, Bcl-2 and caspase-3 were detected by Western blot, mRNA level of PCNA and FAP-αwere detected by Real-time PCR. The proliferation ability of NC group and siRNA group of cells were analyzed by CCK8 assay. Results: ①Forty-eighth after transfection, mRNA and protein expressions of FAP-αin siRNA group were remarkably reduced as compared with those in NC and Blank group (P<0.05); Compared with NC group, the expression of caspase-3 was higher (P<0.05), while that of Bcl-2 was lower (P<0.01). The expression of PCNA mRNA was lower than NC group (P<0.01). ②The OD450 value of siRNA group was significantly lower than that in the NC group 24, 48, 72 and 96 h after transfection (P<0.05). Conclusion: siRNA-mediated fibroblast activation protein alpha knock-down can inhibit the proliferation of U87 glioma cells and promote apoptosis of glioma cells. 2014 Vol. 44 (4): 241-244 [Abstract] ( 728 ) HTML (1 KB)  PDF (825 KB)  ( 809 ) 245 Preparation and identification of polyclonal antibodies against M13 phage TAN Hui1, WANG Bingbing2, LU Xulian2, HOU Bolong2, LIN Xiaoyun2, LIU Qiaoqiong2, ZHANG Lifang2, CHEN Xiaofei1. Objective: To prepare and characterize rabbit-anti M13K07 helper phage polyclonal antibodies.  Methods: The helper phage was amplified and used to immunize rabbits to obtain polyclonal antibodies. Begin from the first week, the same immunization schedule of subcutaneous multipoint injection was repeated three times at 2 weeks intervals. Begin from 0 week, take blood serum collection from ear vein every other week. The immunogenicity of the M13K07 helper phage was detected with Dot Immunobinding Assay and indirect ELISA was used to demonstrate the titer of antiserum in the immunized rabbit. Results: It showed that the specific rabbit antibody against M13K07 helper phage could combined with M13K07 with Dot Immunobinding Assay. Indirect ELISA analysis showed that the helper page can stimulate high levels specific IgG antibody. Along with the increase of immunization times, antibody levels keep rising, reached the peak at the 6th week. The titer of the polyclonal antibody was approximately1:3 200. Conclusion: In this study, polyclonal antibodies against this M13K07 helper phage is successfully generated and such helper phage polyclonal antibodies is important reagent for functional analysis for phage. 2014 Vol. 44 (4): 245-247 [Abstract] ( 829 ) HTML (1 KB)  PDF (802 KB)  ( 1017 ) 248 Effects of HBV S-ecdCD40L on phenotype and function of dendritic cells from hepatitis B virus genome transgenic mouse  WU Wenzhi, XU Ying, WU Jinming, ZHANG Huan Objective: To examine the influence of expression of HBV S-ecdCD40L on the maturity differentiation and function of the dendritic cells (DC) from hepatitis B virus (HBV) genome transgenic mouse models, and to explain the possible mechanism. Methods: HBV transgenic mice were injected with pcDNA3.1-S-ecdCD40L, pcDNA3.1-S, pcDNA3.1 and PBS respectively, (each 100μg/25 g). Vaccination was performed on, day 15 and day 30. Six weeks after first injection sera were collected. Isolate DC and examine the phenotypic and functional changes, and the HBV DNA copies in HBV transgenic mice serum were detected by real-time PCR. Results: Compared with other three control groups, DC from injection with pcDNA3.1-S-ecdCD40L mice enhanced expression of costimulatory molecules (CD80, CD86, MHC II), Tollrike receptor 4 (TLR4), proinflammatory cytokine (IL-12p70) and also the capacity to induce allogeneic lymphocytes proliferation. And the average copies of serum HBV DNA were lower than those of the other three control groups. Conclution: The study in vivo supports the concept that the recombinant plasmids pcDNA 3.1-S-ecdCD40L-mediated HBV S-ecdCD40L expression may be a useful strategy for activating DC抯 maturity and enhancing its functions. 2014 Vol. 44 (4): 248- [Abstract] ( 646 ) HTML (1 KB)  PDF (857 KB)  ( 731 ) 252 Comparative study between guinea pig ovarian fragments and isolated preantral follicles cryopreservation with vitrification ZHANG Yue1, CHEN Wenming2, XIE Chichi2, XIE Shuangshuang2, SUN Junhui2, XU Zhihui2, LIU Jun3, ZHOU Ying2. Objective: To compare the follicle morphology, survival rate and in vitro growth potential of guinea pig ovarian fragments and isolated preantral follicles after vitrification. Methods: Five- weeks aged female guinea pigs were used. Two vitrification solutions (solution A and B) were applied to vitrify guinea pig ovarian fragments and isolated preantral follicles. The toxicity and cryopreservation efficiency of the two solutions were tested on both fragments and isolated preantral follicles. The ovaries were observed using hematoxylin and eosin staining; the viability of the follicles was evaluated by trypan blue. The isolated preantral follicle was cultured in vitro to evaluated the follicle development potential. Results: Based on morphological analyze, the majority follicles were normal in fragments cryopreservation, but the abnormal follicle were higher than fresh group, P<0.05. The recover rates of follicle after being thawed using trypan blue were 66.1% in solution A fragment vitrification group, significantly lower than that in solution B follicle vitrification group (78.5%) and fresh group (87.5%), P<0.05. No significance was found among other groups. During in vitro culture, the vitrified fragments showed poorer develop potential than follicle vitrification groups, typically solution A fragment group, and the follicle survival rate by day 6 was lower than (20.0%), significantly lower than fresh group (60.0%) and solution B follicle vitrification group (50.0%), P<0.05. Conclusion: The results show that the vitrification can preserve guinea pig ovarian fragments and isolated preantral follicles, the latter one achieved better results. Solution B was more efficient in preserving guinea pig follicles. The follicles show growth potential during in vitro culture after being thawed. 2014 Vol. 44 (4): 252-257 [Abstract] ( 591 ) HTML (1 KB)  PDF (980 KB)  ( 658 ) 258 The mechanism of elemene and PD98059 inducing apoptosis of human gastric cancer cell line SCG- 7901 YU Yaojun, SHENG Weiwei, YE Haibo, TU Yangyang, LIU Shuai, SUN Weijian, YOU Tao, WANG Feihai, ZHENG Zhiqiang. Objective: To explore the effect of elemene and PD98059 on the apoptosis of human gastric cancer cell line SGC-790l and the relationship of ERK1/2, P38MAPK signal pathway. Methods: SGC-790l cells were treated with elemene and PD98059. MTT assay was used to detect the proliferation of SGC-790l cells. The expression of ERK1/2, phosphorylation REK1/2 (p-ERK1/2) and phosphorylated P38 (p-P38) was detected by western-blot.The bcl-2 mRNA and bax mRNA was detected with RT-PCR. The gastric cancer cell apoptosis index was detected by TUNEL method. Results: Elemene and PD98059 alone effectively inhibited the proliferation of gastric cancer cells SGC-7901, and the former was in a dose-dependent and time-dependent manner, the latter was in a time-dependence but no in dose-dependent manner, and the combined effects of the two drugs on gastric cancer cell proliferation inhibition was significantly greater than that of single agent (P<0.05). The expression of p-ERK1/2 protein was increased (P<0.05), and the total ERK1/2 was no significant change with the concentration of elemene increased. The expression of p-P38 of elemene group, PD98059 group and the combined group were significantly higher than that of the control  group (P<0.05), and the combined group was the highest (P<0.05). The mRNA of bcl-2 expression was down-regulated while the expression of bax mRNA was up-regulated and both changes had good dose-dependent tendency, and the combined group was most significantly. Cell in experimental groups apoptosis rate was higher than that in the control group (P<0.05), and in a dose-dependent manner with elemene (P<0.05).The apoptosis rate of elemene+PD98059 group was significantly higher than that of the single-action group (P<0.05). Conclusion: Elemene and PD98059 can inhibite the proliferation of gastric cancer cells SGC-7901, and the former was in a dose-dependent and time-dependent manner. Elemene and PD98059 can also promote the apoptosis of gastric cancer cells. Elemene promotes apoptosis and inhibites the proliferation of gastric cancer cells by up-regulating the expression of p-ERK1/2 signaling pathway and p-P38MAPK signaling pathway. PD98059 inhibites the ERK1/2 phosphorylation, but can promote the expression of p-P38 MAPK signaling pathway, then inhibites the proliferation and promotes gastric cancer cell apoptosis. 2014 Vol. 44 (4): 258-263 [Abstract] ( 631 ) HTML (1 KB)  PDF (1110 KB)  ( 782 ) 264 Effect of dihydromyricetin against H2O2 induced injury in human umbilical vein endothelial cell LIU Yunxiao, PAN Xiaoqiong, HU Zhen. Objective: To investigate the effects of pretreatment with DMY on H2O2 induced damage in human umbilical vein endothelial cell (HUVECs) and to explore the related mechanism. Methods: Groups were set as below: normal control group, H2O2 group, groups for low, medium and high dosage of dihydromyricetin (DMY) respectively.Different concentrations of DMY (5μg·mL-1, 20μg·mL-1, 80μg·mL-1) precultured HUVECs for 12 hours,then 0.5 mmol·L-1 H2O2 was used to invervene for 12 hours, using MTT to detect cell viability, Hoechst33258 for apoptotic nuclear staining, the supernatant was taken to determine SOD activity and MDA content. Flow cytometry was used to determine intracellular reactive oxygen species (ROS) and calcium levels. Results: DMY could significantly protect H2O2 (0.5 mmol·L-1) after H2O2-induced injury in HUVECs (P<0.05). We found SOD activity in the supernatant of increased while MDA content and intracellular ROS expression including calcium concentration all decreased of which results became obvious when in 5μg·mL-1 solution of DMY. Conclusion: DMY can reduce intracellular ROS expression through intracellular Ca2+ pathway, DMY concentration of 5μg·mL-1 has the most obvious effect. 2014 Vol. 44 (4): 264-267 [Abstract] ( 611 ) HTML (1 KB)  PDF  (0 KB)  ( 349 ) 268 Correlative study on retinal vascular lesions on systemic lupus erythematosus and disease activity XIA Xiaoru1, XU Hong2, QIN Wei1, LIU Yinhe2, ZHU Xiaochun Objective: To observe the early characteristics of systemic lupus erythematosus (SLE) patients with retinal vascular lesions and to investigate correlation between the degree of retinal vascular lesions and disease activity. Methods: The SLE patients were divided into 2 groups: experimental group (patient with retinal vasculopathy) and control group. All the patients were checked of vision, slit lamp, visualfield, OCT, fundus photograph, fundus fluorescence angiography (FFA) and then rated by the BenEzra scoring system, evaluating the relationship between ocular manifestation and lupus activity. Results: The early stage SLE patients showed different levels of retinal vascular lesion, including cotton wool spots, blood vessels expand circuity, retinal hemorrhage which may lead to proliferative retinopathy. Between two groups, patients with retinopathy have higher positive rate in Raynaud抯 phenomenon and ds-DNA (P<0.05). There was significant difference in the SLEDAI scoring (Z=-2.695, P=0.007). There was positive correlation bewteen the BenEzra score and SLEDAI scores (r= 0.836, P<0.001). Conclusion: Retinal vascular lesions can happen in early SLE, particularly with Raynaud抯 phenomenon; BenEzra plays important role in early judgment of desease activities and vasculitis on SLE. 2014 Vol. 44 (4): 268-271 [Abstract] ( 653 ) HTML (1 KB)  PDF (773 KB)  ( 758 ) 272 Expression of CD44V6, p-Src and Ki67 and their correlation in gastric cancer WANG Yonghui1, MAO Weibo2, LOU Jian1, ZHOU Yuefen1. Objective: To study the expression of the cancer stem cell marker CD44V6, non-receptor tyrosine kinase p-Src and Ki67 proliferation index in gastric cancer and paracancer tissues, analyze their relationship with clinicopathological parameters and prognosis of gastric cancer, and further explore the possible link between the three. Methods: Immunohistochemical staining (EnvisionTM) was used to detect CD44V6, p-Src (Y419) and Ki67 in 85 cases of gastric carcinoma and 41 cases of corresponding paracancer tissues. Differences in the expression of the three and the association with the clinicopathological parameters were analyzed. The possible links that exist between the three were explored. Results: The expression rate of CD44V6, p-Src (Y419) and Ki67 in the gastric cancer tissues was significant higher than that in the adjacent non-tumorous tissues. The expression of CD44V6 was significantly related with tumor size, depth of invasion and pTNM classification (P<0.05); The  high expression of p-Src (Y419) was significantly related with tumor size, tumor differentiation and depth of invasion (P<0.05). The high expression of Ki67 was significantly related with tumor size, Lauren classification (P<0.05). Univariate survival analysis revealed a significant association between lower 2-years survival time and CD44V6 expression, p-Src (Y419) and Ki67 high expression. Multivariate analysis revealed that CD44V6 expression and elevated p-Src (Y419) and Ki67 expression were not an independent poorly prognostic parameter for 2-years survival time. The expression of p-Src and Ki67 was higher in the CD44V6 positive expression gastric cancer tissues than that in CD44V6 negative expression gastric cancer tissues. There was positive correlation among CD44V6, p-Src and Ki-67 expression (P<0.05). Conclusion: The expression of CD44V6, p-Src and Ki67 maybe a biological marker for poor prognosis for gastric cancer. 2014 Vol. 44 (4): 272-277 [Abstract] ( 676 ) HTML (1 KB)  PDF (945 KB)  ( 793 ) 278 Analysis of risk factor and establishment of scoring system for predicting tachyarrhythmia after thoracic surgery  LIN Yueyue1, YOU Jie2, LIN Xiaoming1, LI Xueping1, PAN Danhong1, PAN Xiaoyun Objective: To analysis the risk factor of tachyarrhythmia after general t horacic surgery (GTS), establish the scoring system that predicts the occurrence rate of tachyarrhythmia. Methods: Retrospective study was performed on 412 patients who underwent GTS in our department. Results: Occurrence rate was 35.6%, the risk factor of tachyarrhythmia after GTS included preoperative lung function (P<0.01), preoperative cardiac function (P<0.01), surgery (P<0.01), surgery time (P<0.01). Calculated the possible function of tachyarrhythmia occurrence rate and established the scoring system with abnormal preoperative lung function (yes 10, no 0), abnormal preoperative cardiac function (yes 8, no 0), pulmonectomy or oesophagectomy (yes 26, no 0), surgery time (≥4 h 12, <4 h 0). 26 predicted the tachyarrhythmia, with sensitivity 0.924, specificity 0.514. Occurrence rate blow 26 was 14.6%, above 26 was 52.9% (P<0.01). Conclusion: The scoring system that predicts tachyarrhythmia occurrence rate is simple, convenient and applicable. 2014 Vol. 44 (4): 278-281 [Abstract] ( 612 ) HTML (1 KB)  PDF (700 KB)  ( 645 ) 282 Single-port laparoscopic-assisted percutaneous extraperitoneal closure for indirect inguinal hernia: a prospective clinical controlled study ZHONG Hong ji, WANG Furan, CHEN Yi, ZHAO Junfeng. Objective: To introduce a technique of single-port laparoscopic-assisted percutaneous extraperitoneal closure (SLPEC) using a common epidural needle, and compared with the double-port laparoscopic-assisted percutaneous extraperitoneal closure (DLPEC) prospectively to evaluate the effectiveness and safety for indirect inguinal hernia in children. Methods: Between September 2012 and February 2013, there were 30 consecutive children with indirect inguinal hernia treated with the SLPEC and DLPEC techniques, respectively. Sliding hernia, direct hernia or femoral hernia found during operation was excluded. Data was collected prospectively and analyzed using the SPSS 17.0 software. Results: Two children with sliding hernia were excluded, and there were 28 and 30 children finally included in the SLPEC and DLPEC group, respectively. There was no significant difference of the baseline characteristics between the two groups. The SLPEC group had a slightly longer operative time and a slightly higher conversion rate than that the DLPEC group, although only the difference of the bilateral operative time was significant. There was no significant difference of the time to resume feeding and the time to resume full activity, no need for acetaminophen, and no postoperative complication. The score of incision scars was significantly lower for the SLPEC group than those for the DLPEC group. Conclusion: SLPEC is proved to be a safe and effective surgical procedure for indirect inguinal hernia in children. Compared to DLPEC, it provided a better cosmesis of incision, while the operation diffculty increased.  2014 Vol. 44 (4): 282-285 [Abstract] ( 674 ) HTML (1 KB)  PDF (796 KB)  ( 831 ) 286 Factor XII deficiency in two women with recurrent in vitro fertilization-embryo transfer failure XIE Haixiao, WANG Yingyu, ZHOU Wu, WANG Xiuxiu, WANG Mingshan. Objective: To identify the possible correlation of FXII deficiency with recurrent IVF-ET failure. Methods: Prothrombin time (PT), activated partial thromboplastin time (APTT), FXII procoagulant activity (FXII:C), Plasminogen activity, D-Dimer and other coagulant parameters were detected. Exons 1-14, boundary introns including the splice junctions of the FXII gene were amplified with Polymerase chain reaction (PCR). The PCR products were purified and sequenced directly. If a gene mutation was found, then reverse sequence to confirm it. Fifty heathy persons as normal controls. Results: Both APTT in the two patients were significantly prolonged. And the FXII:C values of the patients were low. Patient 1 was found heterozygous deletion mutation g.6753delACA in exon 9 and 46T/T genetype in the promoter region of FXII gene. Patient 2 was found heterozygous insert mutation g.7142 insertC in exon 10 and 46C/T genetype. The mutation of FXII g.6753delACA was a new mutation. Conclusion: FXII deficiency may contribute to impairment of fibrinolytic activity. Decreased FXII activity may be one of the important reasons of recurrent IVF-ET failure. 2014 Vol. 44 (4): 286-289 [Abstract] ( 681 ) HTML (1 KB)  PDF (852 KB)  ( 653 ) 290 The application of late gadolinium enhancement in coronary artery disease XU Huazhi, LI Jiance, WANG Meihao, WANG Suyuan, CHEN Yongchun, WEN Caiyun, CHEN Weijian. Objective: To explore the application in coronary artery disease with the use of late gadolinium enhancement on cardiovascular magnetic resonance (LGE-CMR) images. Methods: A total of 19 cases diagnosed as coronary artery disease (CAD) with LGE-CMR images in our hospital between January 2010 to March 2013. were accepted (15 men, 4 women, ages range from 36 to 79 years, median age 57 years), including 5 cases of coronary atherosclerosis, 14 cases of coronary atherosclerotic heart disease. There were 5 patients with acute myocardial infarction and 5 patients with chronic myocardial infarction in the group of coronary atherosclerotic heart disease. 2 radiologists evaluated whether LGE or not, LGE localizations (subendocardial, transmural, mid-wall) and LGE morphology (spot/spots, linear, patchy) independently and blindly, Kappa test for consistency. Results: LGE present/absent, LGE localization and morphology had a good consistency (Kappa=0.908, 0.733, 0.724, P all <0.05) between 2 radiologists. LGE signs were observed in 84.2% (16/19) CAD cases, and 81.25%(13/16) located in subendocardial (7/16) or transmural (6/16). 43.75% (7/16) patchy, 31.25% (5/16) lines and 25.00% (4/16) spot/spots enhancement were seen. Conclusion: LGE-CMR is helpful to detect cardiac lesion and its range on CAD patients and also useful to identify the extent of myocardial lesions. 2014 Vol. 44 (4): 290-293 [Abstract] ( 694 ) HTML (1 KB)  PDF (841 KB)  ( 841 ) 294 Prosthetic effect of recombinant human basic fibroblast growth factor combined with silvadene cream on the healing of chronic and refractory wound PAN Debiao, YE Guanxiong, WU Chengjun, XU Shengqian, WANG Shi, QIN Yong. Objective: To explore and discuss the therapeutic effect of chronic and refractory wound by recombinant human basic fibroblast growth factor combined with silvadene cream. Methods: Eighty cases with chronic and refractory wound were selected from Lishui People抯 Hospital between July 2008 and March 2013. These patients were randomly divided into 4 groups: group A receiving regular therapy, group B receiving recombinant human basic fibroblast growth factor, group C receiving silvadene cream, group D receiving recombinant human basic fibroblast growth factor combined with silvadene cream, 20 patients in each group. The wound healing period and the wound healing rates in the 4 groups were recorded. Results: As compared with group A, B, and C, the wound healing period was significantly decreased (P<0.05), the wound healing rate in day 7, 11, 14, 17, and 21 was obviously inereased (P<0.05). Conclusion: Recombinant human basic fibroblast growth factor combined with silvadene cream can promote  the healing of chronic and refractory wound. 2014 Vol. 44 (4): 294-296 [Abstract] ( 766 ) HTML (1 KB)  PDF  (0 KB)  ( 532 ) 296 Analysis of death causes in patients with maintenance hemodialysis in Wenzhou areas WANG Yanhui, LIU Yi, ZHENG Chenfei, ZHOU Qiongxiu, CAI Xiaoqing, XU Yulan. Objective: To analyze the death causes and the characteristics of the maintenance hemodialysis (MHD) patients died in 18 hospitals of Wenzhou areas of China from January 2010 to December 2012. Methods: The causes of death in patients with MHD for more than 3 months were retrospectively analyzed. These patients, were divided into 3 groups abased on age: young group (20 to 44 years old), middle age group (45 to 64 years old), elderly group (≥65 years old). Dialysis age, end stage renal disease (ESRD) causes and the biochemistry indexes at the beginning and within one year prior to death were analyzed among the three groups. Results: Of the 269 death cases, 166 cases were male and 103 female, with the mean age of 66.9±13.7 years old (21 to 93 years old). 20 cases were in young group, 80 in middle age group and 169 in elderly group. Diabetes was major ESRD cause in all cases. Cerebral vascular diseases and cardiovascular diseases were main death causes among three groups, and there was no significant difference among them. Systemic failure was the secondary death cause in elderly group. Serum creatinine (Cr) and blood urea nitrogen (BUN) at the beginning and within one year prior to death in elderly group were lower than that in the other two groups, the serum albumin was the same as sCr and BUN within one year prior to death. Conclusions: Most of the death cases with MHD in this study are older than 65 years of age in Wenzhou area. Diabetes is a major ESRD cause in all cases. Cerebral vascular diseases are the first death causes and cardiovascular diseases are the second among three groups, Systemic failure as death cause in elderly group is more common. Nutritional status in elderly patients is worse than that in young and middle age patients. 2014 Vol. 44 (4): 296-299 [Abstract] ( 661 ) HTML (1 KB)  PDF (744 KB)  ( 748 ) 300 Comparison of curative effect of duloxetine and paroxetine in treatment of generalized anxiety disorder with pain ZHAO Yongzhong, YANG Chuang, WANG Weiqian, CHEN Hong Objective: To evaluate the efficacy and safety of duloxetine and paroxetine in the treatment of generalized anxiety disorder with pain. Methods: Eighty seven patients were randomized into two groups. Forty four patients in duloxetine group were administered with duloxetine 60 mg/day and forty three patients in paroxetine group were treated with paroxetine 20 mg/day for 6 weeks. Efficacy were assessed with Visual Analogue Scale(VAS) for pain, Hamilton Anxiety Scale (HAMA) for anxiety, and Treatment Emergent Symptom Scale (TESS) for side effect. Results: As the duration of treatment, the VAS scores in the both groups were reduced significantly than that before treatment. At the end of 2, 4, 6 weeks after treatment, the VAS scores in duloxetine group were lower significantly than that in paroxetine group (P<0.05). The scores of HAMA and HAMA-somatic in duloxetine group were reduced significantly at the end of 2, 4 weeks after treatment than that in paloxetine group (P<0.05). The effective rate of treating the anxiety were 60.98% and 58.54% (P>0.05), and the recovery rate were 34.15% and 26.83% (P>0.05) in duloxetine group and paroxetine group, respectively. Conclusion: Duloxetine is an effective and safe antidepresent as paroxetine in treatment of generalized anxiety disorder with pain. Duloxetine is more effective against pain, and work faster than paroxetine in aspect of anxiety and pain. 2014 Vol. 44 (4): 300-302 [Abstract] ( 1106 ) HTML (1 KB)  PDF (621 KB)  ( 774 )

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