温州医科大学学报
 
        Home  |  About Journal  |  Editorial Board  |  Instruction  |  Subscribe  |  Messages Board  |  Contact Us  |  中文
温州医科大学学报
 
 
 
 
Forthcoming Articles
Current Issue
Archive
Advanced Search
Read Articles
Download Articles
Email Alert
2014 Vol. 44, No. 3
Published: 2014-03-25
 
 
157 Long-term programming effects of obesity on the sympathoadrenal system in female rats
WU Xueqing1, YANG Simeng1, LI Yuehua1, ZHOU Qiangyong1, CHEN Haiyan1, LI Haiying2
Objective: To investigate the long-term programming effects of obesity on the sympathoadrenal system in female rats. Methods: The litter size was adjusted to 4 pups per litter for the treatment group and 12 neonates per dam in the controls at postnatal day 0. Body weights were measured weekly. Vaginal opening and vaginal cytology smears were monitored from postnatal day 28. At the 10 weeks of age, the sympathetic nerve activity in adrenals and ovaries were detected by immunohistochemistry and the expression of TH mRNA in the locus coeruleus were investigated by in situ hybridization. Results: As compared to normal litters, the small litter rats were obesity throughout life. The puberty onsets were obviously advanced (35.5±0.8 d in treatment groups vs.37.6±0.3 d) in controls. The immuno-staining of TH were significantly enhanced in both the ovaries and adrenals in small litters. The level of TH mRNA in the locus coeruleus was significantly increased in small litters. All the differences were statistically significant (P<0.05). Conclusion: Obesity programmed the reproductive function of female rats by up-regulation of the sympathoadrenal system.
2014 Vol. 44 (3): 157-160 [Abstract] ( 599 ) HTML (1 KB)  PDF (799 KB)  ( 880 )
161 The inhibition effect of relaxin for TGF-βinduced endothelial to mesenchymal transition in vitro
CHEN Xiao1, ZHOU Hao1, ZHOU Xi1, CAI Jiejie1, CHEN Lingzhi2, ZHENG Gaoshu1, HUANG Weijian1, ZHANG huaiqin1.
Objective: To investgate the effect of relaxin which inducing endothelial to mesenchymal transition in vitro. Methods: Endothelial cells were isolated from human umbilical vein endothelial cells (HUVECs). TGF-β10 ng/mL was added to medium for inducing endothelial to mesenchymal transition. Pretreatment relaxin 100 ng/mL and 200 ng/mL was added to medium, then co-cultured with TGF-β10 ng/mL for 48 h. The changes of morphology were observed under an inverted phase contrast microscope. CCK-8 assay was used to evaluate the cell proliferation as well as Transwell tested cell migration. The expression of CD31, vimentin, ve-cadherin andα-SMA were determined by immunofluoresence staining and western blot analysis. The co-expression of CD31 with vimentin and VWF withα-SMA were also determined by immunofluoresence. Results: A paving stone-like growth was observed in control group while TGF-βinduced more spreading and migrating cells which was called EMT. However, this transition could be prevented by relaxin. Compared with the control group, the proliferation and the mobility were increased by TGF-β(P<0.05), but declined when combining with relaxin compared to adding TGF-βonly (P<0.05). Compared with the control group, the specific protein of endothelial CD31 (0.36adding TGF-β, but the specific protein of mesenchymal vimentin (0.72±0.102 VS 0.21±0.081) and α-SMA(0.88±0.084 VS 0.24±0.046) were significantly increased. However, relaxin could inhibit the phenotype switch. Compared with the TGF-βinducing group, CD31 (0.67±0.09, 0.59±0.12 VS 0.36±0.076) and ve-cadherin(0.85±0.09, 0.72±0.064 VS 0.54±0.046) were significantly increased, but vimentin (0.56±0.011, 0.48±0.06 VS 0.72±0.102) andα-SMA (0.65±0.081, 0.54±0.032 VS 0.88±0.084) were significantly decreased when combining with relaxin. Besides, it exhibited dose-dependent. Last but not least, the number of double positive cells was largely increased in TGF-βinducing group compared to the control group. And compared with the TGF-β group, double positive cells performing mesenchymal are declined while performing endothelial ones are increased in the combined group. Conclusion: Relaxin exhibits the inhibition effect for TGF-βinduced endothelial to mesenchymal transition in vitro.
2014 Vol. 44 (3): 161-165 [Abstract] ( 714 ) HTML (1 KB)  PDF (922 KB)  ( 880 )
166 Evaluation of left ventricular diastolic synchronism in patients with hypertrophic cardiomyopathy by two dimensional speckle tracking imaging
LIU Min1, LIU Liwen2, CAO Hongyan3, TUO Shengjun4, ZUO Lei2, YANG Liping2, TIAN Xinqiao
Objective: To evaluate diastole synchrony of the different segments of left ventricular in patients with hypertrophic cardiomyopathy by two dimensional speckle tracking imaging. Methods: Forty-seven patients with HCM and thirty-three healthy volunteers were studied by two dimensional speckle tracking imaging.The peak time of early and late diastolic longitudinal, radial, circumferential strain rate were measured from the start point of QRS wave of electrocardiogram. The left ventricular segmental standard deviation (TESRL-SD, TASRL-SD, TESRR-SD, TASRR-SD, TESRC-SD, TASRC-SD) were calculated, which were served as diastolic synchronism indexes in assessing LV diastolic aspect. Results: TESRL-SD, TESRR-SD, TESRC-SD and TASRL-SD were significantly higher in patients with HCM than those in normal controls. TASRR-SD and TASRC-SD were higher in patients with HCM than those in normal controls, but the difference had no statistical significance. Conclusion: Left ventricular diastole dyssynchrony can be evaluated accurately in patients with hypertrophic cardiomyopathy by two dimensional speckle tracking imaging.
2014 Vol. 44 (3): 166-168 [Abstract] ( 629 ) HTML (1 KB)  PDF (711 KB)  ( 708 )
169 Correlative analysis of asthenospermia and the mutation site of 12338, 12358 and 12406 in mtND5
LUO Huiying1, LI Chuanlian2, LOU Zhefeng1, ZHENG Jiujia3, ZHANG Liya3, JIN Longjin1.
Objective: To investigate the correlation between asthenospermia and the mutation site of 12338, 12358 and 12406 in mtND5. Methods: Fifty-five semen samples from patients with asthenospermia and 33 from healthy donors based on WHO criteria were collected and analyzed, mutation frequency of 12338, 12358 and 12406 in mtND5 was examined by polymerase chain reaction (PCR) and sequencing. Results: The mutation frequency (m.12338 T>C 10.9%, m.12358 A>G 9.09%, m.12406G>A 12.7%, total 32.73%) in asthenospermia group was higher than the frequency in control group (9.09%, 3.03%, 3.03% and 15.15%), but there was no significant difference between them (P>0.05). However, the percentages of grade a (20.63±13.63) and grade (a+b) (29.66±17.08) sperm in mutation samples (with m.12338 T>C or m.12358 A>G or m.12406 G>A) were significantly lower than that in non-mutation samples (30.61±18.87 and 41.44±22.47) (P<0.05). Conclusion: These mutations of m.12338 T>C, m.12358 A>G and m.12406 G>A in human sperm may have some correlation with sperm motility, but may not be the specific mutation site of asthenospermia.
2014 Vol. 44 (3): 169-172 [Abstract] ( 624 ) HTML (1 KB)  PDF (853 KB)  ( 867 )
173 Contamination levels of environmental estrogens in foods and risk assessment in Wenzhou
ZHAO Nana1, YING Li2, SUN Fangyun1, LONG Jia3, HE Dongliang3, YING Chenjiang3, YE Xiaolei1.
Objective: To determine the levels of environmental estrogens (ESs) in foods in Wenzhou, and perform an assessment of the health risk of ESs. Methods: Randomly selected items from categories of meat, fish/shellfish products, vegetables and cereals of the city and the concentration of ESs, such as nonylphenol (NP), bisphenol A (BPA), ethinylestradiol (EE2) and estradiol (E2), were determined by LC-MS/MS. Daily dietary exposure and health risk index of each estrogen were  calculated considering the dietary pattern of the local people. Results: ESs were detected in all samples, the highest detection rate was of NP (100%) while the lowest was of EE2 (49.3%). Four ESs were simultaneously detected in about 1/4 of the samples and two or more ESs were detected in 96.6% of foods. The average dietary exposure risk index of NP, EE2, and BPA were 1.09×10-2, 0.41, and 1.84×10-4 and the maximum dietary exposure risk index of NP, EE2, and BPA were 2.88×10-2, 1.20, and 8.85×10-4, respectively. Conclusion: Four ESs are detected in almost every food samples; The health risk index of NP, E2 and BPA is less than 1 in food samples in Wenzhou. But the exposure level of E2 may have risk to local residents.
2014 Vol. 44 (3): 173-176 [Abstract] ( 618 ) HTML (1 KB)  PDF (821 KB)  ( 821 )
177 Associative analysis of CYP1B1 genetic polymorphisms with laryngeal cancer susceptibility among northern Han Chinese
LIN Faming1, JIN Jianhua2, XUE Jing3, BAO Qiyu4, LIU Chaobing5, NI Liyan1.
Objective: To explore the association between functional polymorphisms and combined effect in the CYP1B1 (CYP1B1*2 142 C/G, CYP1B1*2 355 G/T, CYP1B1*3 4326 C/G) enzyme genes and the susceptibility of laryngeal cancer in northern Han Chinese. Methods: A case-control study was performed for 200 northern Han Chinese patients diagnosed with laryngeal cancer and 200 healthy control individuals. The genetic polymorphisms of CYP1B1*2 142 C/G, CYP1B1*2 355 G/T, CYP1B1*3 4326 C/G were examined for the individuals by multi-PCR and Matrix Assisted Laser Desorption Ionization-Time of Flight (MALDI-TOF MS) approach. The association of these genetic polymorphisms, smoking, drinking, and the combinations of these factors with the susceptibility of laryngeal cancer was statistically evaluated. Results: The frequencies of CYP1B1*2 355 G/T, CYP1B1*3 4326 C/G gene polymorphisms in the patient cohort were significantly different with those in the control cohort (P<0.001, P=0.007 respectively). Compared to the control cohort, a higher risk laryngeal cancer was observed for the carriers of variant genotypes of CYP1B1*2 355T(adjusted OR=2.281,95%CI: 1.142-3.516, P<0.001) and the mutation allele CYP1B1*3 4326G carriers had lower laryngeal cancer risk (adjusted OR=0.571, 95%CI: 0.370-0.882, P=0.011). Polymorphisms at these three loci presented a joint effect. The haplotype C142T355C4326 was associated with a significantly higher laryngeal cancer risk (adjusted OR=3.180, 95% CI:1.760-5.746, P<0.001) compared to the haplotype C142G355C4326. The adjusted ORs for the individuals with GT/TT genotype at CYP1B1*2 355 G/T were 2.080 (95% CI: 0.742-5.830, P<0.001) and 6.322 (95% CI: 2.541-15.725, P<0.001) respectively for the non-smokers and smokers. Conclusion: Genetic polymorphism of CYP1B1 is associated with risk of laryngeal cancer. CYP1B1*2 355T increase the laryngeal cancer risk and CYP1B1*3 4326G reduces the risk. The association between CYP1B1*2 142C/G polymorphism with the risk of laryngeal cancer dose not found. These polymorphisms showed a joint effect on the susceptibility to laryngeal cancer, presenting as higher risk with more risky factors included. Environmental factors, smoking and drinking, showed a non-collaborative effect with two risky genetic factors.
2014 Vol. 44 (3): 177-182 [Abstract] ( 587 ) HTML (1 KB)  PDF (876 KB)  ( 724 )
183 Effects of antisense oligonucleotide targeting microRNA-155 on breast carcinoma cell line MDA-MB-157 and the implanted tumor in nude mice
ZHENG Shurong, GUO Guilong, HUANG Qidi, HUANG Duping, YOU Jie, HUANG Guanli.
Objective: To explore the effects of antisense oligonucleotide targeting miR-155 on proliferation and apoptosis in MDA-MB-157 cells and the growth of implanted tumor in nude mice. Methods: 2扥me modified antisense oligodeoxynucleotide targeting miR-155 was synthesized and then transfected into MDA-MB-157 cells by LipofectamineTM 2000. Transfection efficiency was detected by laser confocal microscope. Real-time RT-PCR method was used to detect the expression of miR-155. Cell counting Kit-8 (CCK-8) assay was used to detect the proliferation of MDA-MB-157 cells and cell apoptosis rate was measured by flow cytometry. The effects of miR-155 Antisense oligonucleotide (miR-155 ASO) on the transplanted tumor were assessed in nude mice. Results: Transfection efficiency detected by laser confocal microscope was higher than 80%. The level of miR-155 expression was significantly decreased (P<0.05) in the cells transfected with miR-155 ASO, compared with that in cells transfected with negative control. After being transfected with miR-155 ASO, the viability of MDA-MB-157 cells reduced greatly (P<0.05) and the number of apoptotic cells increased significantly. Additionally, miR-155 ASO inhibited the growth of transplanted tumor in vivo (inhibition ratio=52.98%) and significantly increased the expression of caspase-3. Conclusion: miR-155 ASO can effectively down-regulate the expression of miR-155, induce cell apoptosis and inhibit cell proliferation and tumor growth by increasing the expression of caspase-3. These findings provide the experimental evidence for using miR-155 as a therapeutic target of breast carcinoma.
2014 Vol. 44 (3): 183-188 [Abstract] ( 663 ) HTML (1 KB)  PDF (975 KB)  ( 769 )
189 Effect of 3-methyladenine on autophagy and cell proliferation in 5-Fu-resistant colon cancer cell line SW480
HU Wanle, WANG Lingqin, CHEN Suping, CHEN Rong.
Objective: To investigate the effects of 3-methyladenine on chemotherapy sensitivity of 5-Furesistant colon cancer cell line SW480 and reversing the 5-Furesistant in colon cancer. Methods: Stepwise 5-Fu selection was used to establish the 5-Fu resistant colon cancer cells (100μg/L). The cells were dealed with 3-methyladenine (10μmol/L) fistly and 5-Fu (200μg/L) for 24 hours. Cell proliferation inhibition rate was measured with CCK-8. Apoptosis rate was detected with flow cytometer. Autophagic vacuoles in cells were observed by means of transmission electron microscopy and monodansylcadaverine (MDC). Results: The inhibition rate of 3-methyladenine in the 5-Fu resistant cell autophagy activity was 89.7%, cell proliferation was 9.6%, but apoptosis rate was just 5.7%, were obviously higher than that in 5-Fu resistant cells (P<0.05). After dealing with 3-methyladenine(10μmol/L) fistly and 5-Fu (200μg/L) for 24 hours, the level of cell proliferation inhibition rate was 71.6%±2.9% and the apoptosis rate was 36.6%±2.9%, while dealing with 5-Fu (200μg/L) for 24 hours, the level of cell proliferation inhibition rate was 23.6%±2.9% and the apoptosis rate was 10.3%±2.5%. After dealing with 3-methyladenine, the cell proliferation inhibition rate and apoptosis rate were markedly higher than that of 5-Fu only. Conclusion: The anticancer capacity of 3-methyladenine in colon cancer is weak, but it can reverse the 5-Fu-resistant in colon cancer partly, 5-Fu-induced apoptosis in 5-Fu resistant colon cancer cells may be enhanced by the inhibitor of autophagy.
2014 Vol. 44 (3): 189-192 [Abstract] ( 825 ) HTML (1 KB)  PDF (841 KB)  ( 842 )
193 Analysis of related risk factors in cardiovascular implantable electronic device infection
FANG Danhong, WU Gaojun, LI Haiying, LIN Jie, HUANG Weijian.
Objective: To determine the the incidence of infection in cardiovascular implantable electronic device (CIED) in our heart centre, and analyse the risk factors associated with infection. Methods: Thirteen years (2000-2012) of cases of CIED infection were analyzed retrospectively. The clinical presentation and base characteristics of these patients were analyzed, risk factors associated with infection were assessed. Results: 1 817 cases (male:female=1.4:1) were implanted cardiac electronic device [permanent pacemakers, cardiac resynchronization therapy (CRT) and implantable cardioverter defibrillator (ICD)]. 16 patients (male:female=3:1) occurred CIED infection, the incidence was 0.9%. Fifteen (93.8%) cases were identified as pacemaker pocket infection. Microorganisms were detected in 68.8% cases, Of which 90.9% was coagulase-negative staphylococci. Significant risk factors included implantation of ICD/CRT-D (P=0.000), diabetes mellitus (P=0.009) and thyroid hypofunction (P=0.000), odds ratio was 9.12, 4.18 and 37.55, respectively. The procedure of pacemaker replacement did not increase the risk of CIED infection (P>0.05). The body mass index (BMI) of male CIED infection patients, particularly of non-diabetic CIED infection men, was significantly lower than that of non-CIED infection men (P<0.05). Conclusion: The CIED infection rate is 0.9%, the vast majority showed pocket infection and coagulase-negative staphylococci are common pathogens. Implatation of ICD/CRT-D, diabetes mellitus and thyroid hypofunction are related risk factors of CIED infection; lower BMI men are more susceptible to CIED infection.
2014 Vol. 44 (3): 193-196 [Abstract] ( 649 ) HTML (1 KB)  PDF (824 KB)  ( 730 )
197 Testing of activated plasma clotting time to assess the bleeding diathesis of  immune  thrombocytopenia
HE Muqing1, HE Muqun2, LIN Xiaoji1.
Objective: To investigate the value of activated plasma clotting time (APCT) for assessing the bleeding diathesis of immune thrombocytopenia. Methods: There were one hundred and twenty-eight patients of diopathic immune thrombocytopenia. Taking the patient抯 venous blood, the patient抯 blood platelet count and activated plasma clotting time were determined. The patients were devided into two groups by platelet count, one group抯 platelet count was lower than 30×109/L, and the other group抯 platelet count was 30×109/L～79×109/L.Acorrding to the test, we could get APCT. By analyzing APCT and Khellaf bleeding score, we could receive that APCT and Khellaf bleeding score results had linear correlation. Results: We set  APCT critical point was high than ninety seconds. The sensibility to assess the ITP patient抯 bleeding was 100%, the specificity is 91.3% and the false positive rate 8.69%. In the group抯 platelet count was lower than <30×109/L, we could receive that APCT and Khellaf bleeding score results had linear positive correlation, P=0.013, r=0.968. In the group抯 platelet count was about 30×109/L～79×109/L, we could receive that APCT and Khellaf bleeding score results had correlation,P=0.01, r=0.764. Conclusion: APCT has a good sensibility and specificity. It is a good predictive index to assessing the bleeding of ITP.q
2014 Vol. 44 (3): 197-200 [Abstract] ( 687 ) HTML (1 KB)  PDF (747 KB)  ( 803 )
201 Establishment of FAK stable silencing SW620 cells
HONG Weiwen1, SU Guoqiang2, YING Hong'an3, LIN Feng1, LIANG Weidong1.
Objective: To establish the FAK stable silencing SW620 cells. Methods: For production of lentiviruses, 293FT cells were transfected by Turbo transfect, using pLL3.7 FAK and negative pLL3.7, lentivirus-containing media were harvested and used to infect SW620 cells as experimental group and control group, SW620 cells didn抰 infect any lentiviruses as unsettled group. Infection efficiency was obtained by the expression of GFP, and maintained as a pool population. Expression of FAK was detected by RT-PCR and Western blot. Results: Lentiviruses were successfully producted with over 90% infecting rate; cells stable expressing GFP. Expression of FAK were significantly reduced by RT-PCR and Western blot. Conclusion: FAK stable silencing SW620 cells are established.
2014 Vol. 44 (3): 201-204 [Abstract] ( 662 ) HTML (1 KB)  PDF (792 KB)  ( 636 )
205 The correlation between combined detection of CD56, TPO, p63 and CK19 and clinicopathologic parameter in papillary thyroid carcinoma
LI Xiufang, LV Yanting, ZHANG Haiyong, ZHAO Feixing, HU Mengjun.

Objective: To explore the correlation between combined detection of CD56, TPO, p63 and CK19 and clinicopathologic parameter in papillary thyroid carcinoma. Methods: one hundred patients with PTC were detected by immunohistochemistry for the expression of the CD56, TPO, p63 and CK19, and the Chi-square test was utilized to statistical analyze the relation between the above markers and clinicopathologic parameter (gender, age, number, size, location, capsule invasion, lymph node metastasis, histologic subtype of lesion). Results: The expression positive rate of CD56, TPO, p63 and CK19 was 11%, 5%, 17%, 87%  respectively in 100 cases of PTC. No correlation between four markers and the clinicopathologic parameters (gender, age, number, size, location, lymph node metastasis, histologic subtype of lesion), there was no statistically significant difference (P>0.05), but the expression of CD56 in cases with capsule invasion was significantly lower than without capsule invasion, the difference was statistically significant (P<0.05), there was no correlation between expression of the other three markers and capsule invasion, the difference was not statistically significant (P>0.05). Conclusion: There is correlation between the expression of CD56 and capsule invasion, the expression of CD56 in cases with capsule invasion was significantly lower than without capsule invasion, and the low expression can predict the poor prognosis in PTC.
2014 Vol. 44 (3): 205-208 [Abstract] ( 790 ) HTML (1 KB)  PDF (797 KB)  ( 737 )
209 Diagnosis and pathology of CT and MRI and analysis of ovarian endometrioid carcinoma
LI Weida, CHEN Yan, WANG Yuxuan, XIONG Bing, HU Aimei, LUO Weixian, WEI Zhaoxia.
Objective: To study the CT and MRI imaging features and pathology of ovarian endometrioid carcinoma (OEC). Method: CT and MRI imaging features of 8 patients confirmed by surgery and pathology were analyzed retrospectively, and compare with the results of surgery and pathology. Results: Five tumors situated in the left and the 3 tumors others in the right ovary, the tumor diameter ranged from 4.5 to 17.5 cm, the average was 9.2 cm. All cases were cystic-solid lesions, irregular and lobulated contour were found in 6 cases, 2 cases were round or oval contour. Seven cases boundary was partial fuzzy, another one was clear. All the cases showed solid component抯 CT value were 37～46 HU in CT plain scan, the average was 41 HU, cystic component抯 CT value were 14～40 HU, the average was 25 HU. On enhanced CT, solid component was moderately enhancement with the CT value about 61～77 HU, the average was 66 HU in the arterial phase. Parenchyma stage value of CT was 65～78 HU, with an average of 71 HU.Underwent MRI, solid components were equisignal and cystic components showed low signal on T1WI. On T2WI imging, solid components showed slightly high signal and cystic component revealed high signal. Enhanced scanning showed moderately enhancement. In 8 cases of OEC complicated with uterine body membrane carcinoma in 2 cases, of which 1 case underwent MRI examination, manifested as irregular thickening of the uterine body membrane, showed T1WI signal, T2WI is slightly high signal intensity, enhancement after enhanced moderately, one case complicated chocolate cyst of ovary in the same side. Conclusion: CT and MRI can show the OEC pathology feature better. Also they can show the boundary of the tumor and block construction and the encroachment of circa-construction clearly, they possess the important qualitation value and provide reasonable treatment plan for clinic.
2014 Vol. 44 (3): 209-212 [Abstract] ( 840 ) HTML (1 KB)  PDF (834 KB)  ( 690 )
213 Management of failure and malfunction hemodialysis access with interventional radiology
AI Peng, DENG Chaopin, WANG Hua, CHEN Cong.
Objective: To summarized the treating experiences of CDT and PTA in 8 cases with failure and malfunction hemodialysis access. Methods: Eight cases with failure and malfunction hemodialysis access were examined by angiography and treated by interventional management subsequently. Two cases of thrombosis occlusilon were treated with thrombolytic therapy and 6 cases of malfunctioning hemodialysis access were done with PTA.Results: The initial angiogram showed 6 patients with simple stenosis while 2 patients with thrombosis occlusion,with concurrent stenosis in 1 patients. The interventional therapy was successful in 7 cases, 1 of the 7 cases concurrent with stenosis and 6 cases with simple stenosis while the other one confronted failure. Follow up was made in 8 patients concurrent with stenosis without further management and 1 patient failed to conduct  PTA was recanalized afterward. Conclusion: Angiography and subsequent interventional management play a critical role in the diagnosis and treatment of failure and malfunction hemodialysis access.
2014 Vol. 44 (3): 213-215 [Abstract] ( 883 ) HTML (1 KB)  PDF (656 KB)  ( 790 )
216 Treatment of interstitial cystitis with bladder hydrodistention and heparin with alkalinized lidocaine irrigation
CHENG Bin, SHAO Feng, WU Haimei, LIU Yao, CAI Jiangyi, FENG Sike.
Objective: To evaluate the efficacy and security of bladder hydrodistention and heparin with alkalinized lidocaine to treat interstitial cystitis (IC). Methods: Twenty patients with interstitial cystitis received bladder hydrodistention under anesthesia, then bladder irrigation with 2.5×105 U heparin+5 mL of 5% sodium bicarbonate+10 mL of 2% lidocaine+15 mL of 0.9% NaCl injection. The treatment was given 2 times a week, after 4 weeks, the treatment was given 1 time a week, 16 times in all. The interstitial cystitis symptom index (ICSI) and interstitial cystitis problem index (ICPI) were evaluated respectively before the treatment and after the treatment. The treatment effect was evaluated comprehensively. Results: After 6~24 months?follow-up (an average of ten months) on 20 patients, 10 cases showed significant curative effect, 6 cases showed curative effect, while 4 cases showed no effect. The total effective rate was 80%. The ICSI score before treatment was from 10 to 17 (mean, 14.80±1.94), and the ICPI score was from 7 to 15 (13.30±2.11). The ICSI score was decreased to 10.60±3.05 in 4 weeks, 8.55±3.73 in 12 weeks; and the ICPI score was decreased to 10.00±2.20 in 4 weeks, 7.75±2.99 in 12 weeks after treatment, which was significantly distinct before and after the therapy (P<0.05). Conclusion: The treatment of interstitial cystitis with bladder hydrodistention and heparin with alkalinized lidocaine irrigation is effective, and can significantly relieve clinical symptoms and improve the life quality of patients with IC.
2014 Vol. 44 (3): 216-218 [Abstract] ( 766 ) HTML (1 KB)  PDF (675 KB)  ( 833 )
219 Clinical analysis of microscopic polyangiitis with pulmonary involvement in 20 cases
JIN Xiaosheng1, WU Lifeng1, QIAN Xiaoying1, WANG Wantie2
Objective: To analyze the clinical characters of pulmonary involvement in the patients with microscopic polyangiitis. Methods: Twenty cases of MPA with pulmonary involvement were analyzed retrospectively, including clinical manifestation, laboratory test results, chest high-resolution computed tomography, pulmonary function test and pathology. Results: The common symptoms were cough (85%), dyspnea (30%), expectoration (25%) and haemoptysis (20%). Erythrocyte sedimentation rate was elevated in 95% of MPA patients. C-reactive protein was elevated in 55%. The prevalence of positive MPO-ANCA antibodies in MPA patients was 90%, while PR3-ANCA was 5%. Chest HRCT manifestations consisted of ground-glass opacity, interstitial fibrosis, patchy infiltration, pleural effusion and bronchiectasis. Conclusions: The pulmonary is frequently involved in MPA. Pulmonary involvement is the initial manifestation. The chest radiographic and clinical manifestations are nonspecific. Serum ANCA and histopathologic biopsy can help to understand and diagnose MPA with pulmonary involvement early.
2014 Vol. 44 (3): 219-221 [Abstract] ( 581 ) HTML (1 KB)  PDF (722 KB)  ( 682 )
222 Clinical diagnosis and treatment of blunt abdominal trauma combined with intraperitoneal fluid
CAO Haibo, HU Ping.
Objective: To investigate diagnosis and treatment of blunt abdominal trauma combined with intraperitoneal fluid. Methods: One hundred and thirty-five cases of blunt abdominal trauma combined with intraperitoneal fluid patients in our hospital from January 2005 to December 2012 were analyzed retrospectively. Results: Surgery was performed in 104 cases, conservative treatment in 31 cases, death in 37 cases, various complications in 58 cases. Conclusion: The key to increase the rate of cure is happened to pay attention to dynamic observation of post-traumatic symptoms and signs to judge the state of illness was correctly through intraperitoneal indwelling catheter dainage and laparoscopic detection and grasp the surgery indication timely.
2014 Vol. 44 (3): 222-224 [Abstract] ( 580 ) HTML (1 KB)  PDF (706 KB)  ( 719 )
Copyright © Editorial Board of JOURNAL OF WEZHOU MEDICAL UNIVERSITY
Supported by:Beijing Magtech