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2014 Vol. 44, No. 1 Published: 2014-01-25   1 Analysis of serum miR-146a/b by real-time fluorescent quantitative PCR and its preliminarily evaluation as biomarker  LI Chunmei1, ZHENG Chao2, QIAO Minmin1, LV Jianxin1, LI Wei1. Objective: To establish method for the quantitative fluorescence detection of serum miRNAs, and to preliminarily evaluate the potential value of miR-146a/b as serum inflammatory biomarkers. Using serum miR-146a/b, specifically regulating the Toll-like receptor signalling pathway, as the targets. Methods: Serum samples were collected from 20 cases of normal weight children (control group), 20 cases of overweight children (overweight), 20 cases of obese children (obese group) and 50 cases of healthy adults (healthy adult group). Serum total RNAs were extracted by phenol-chloroform extracting methods, copy numbers of serum miR-146a/b were analyzed by SYBR Green real-time fluorescent quantitative PCR techniques, followed by statistical analysis using Graphpad Prism5.0 software and graphics. Results: Copy numbers of serum miR-146a in control group were significantly lower than that in overweight (P=0.0061) and obese groups (P=0.0262), whereas no significant difference was found between overweight group and obesity group (P=0.0656). Area under the ROC curves (AUC) of miR-146a expression in different groups showed as follows: control group vs overweight group AUC=0.8475, P=0.0002; control group vs obese group AUC=0.6050, P=0.2560; overweight group vs obese group AUC=0.5475, P=0.6073. Expression of miR-146b and miR-146a exhibited different trends, copy numbers of serum miR-146b in overweight children were significantly higher than that of normal weight children (P=0.0090) and obese children (P=0.0023); even though the copy numbers of serum miR-146b in obese children were slightly lower than that in control group, the difference had no statistically significant (P=0.1556). The area under the ROC curves (AUC) of miR-146b expression were analyzed as shown in following data: normal group vs overweight group AUC=0.7425, P=0.0087; normal group vs obese group AUC=0.6325, P=0.1517; overweight group vs obese group AUC=0.7825, P=0.0023. The coefficient of variation (CV) values of miR-146a/b copy numbers was largely diverse: CV values of miR-146a copy numbers in control, overweight and obese children were 80.94%, 110.94% and 175.88%, respectively; and that of miR-146b copy numbers were 164.11%, 189.72% and 152.00%, respectively. In healthy adults, the expression patterns of serum miR-146a/b were similar; CV values of miR-146a and miR-146b were 37.86% and 74.82%. Conlusion: The different expression levels of miR-146a in control, overweight and obese groups indicate that miR-146a is correlated with childhood obesity and positively correlated with levels of inflammation in the body. However, the reference range of miR-146a could not be determined on account of its widely varied serum copy numbers and no clear dividing line among different groups. Therefore, the difference of serum miR-146a/b copy number is insufficient to be used in clinical diagnosis, the values of serum miR-146a/b as biomarkers of inflammation still need to be explored further. 2014 Vol. 44 (1): 1-6 [Abstract] ( 789 ) HTML (1 KB)  PDF (2967 KB)  ( 706 ) 7 Tissue extracts from myocardium of rats protects bone marrow stromal cells against hypoxia and serum deprivation-induced apoptosis  LU Di, MAO Chenxi, JIN Peifeng, WANG Jue, SUN Chengchao. Objective: To explore tissue extracts from myocardium of rats protects bone marrow stromal cells (BMSC) against hypoxia/serum deprivation (SD)-induced apoptosis, and investigate its potential mechanism. Methods: The BMSC were isolated from female SD rat and cultured in vitro. The ischemic microenvironment was simulated by hypoxia/SD and tissue extracts from myocardium of rats were extracted by ultracentrifugation. Cell counting kit was used after 24 h hypoxia/SD treatment to observe the cell number of BMSC and to select the proper concentration. The inverted phase contrast microscope observation and heochst 33258 nucleus staining were used to detect the morphological changes of the cells. Western blot was used to detect the expression of caspase-3. Results: The result of cell counting kit showed that with the extracts concentration increasing, the cell number increased. The proper concentration of myocardium tissue extracts was 100μg/mL. Compared with the apoptotic model (AM) group, there were less apoptotic cells in myocardium tissue extracts group and control group. The expression level of cleaved caspase-3 detected was higher in AM group than that in the NMTE and control group. Conclusion: Tissue extracts from myocardium of rats protect BMSC against hypoxia/SD-induced apoptosis. The protective effect is significant when the extracts concentration reached 100μg/mL. The protective effect is related to caspase-3 signal pathway. 2014 Vol. 44 (1): 7-11 [Abstract] ( 614 ) HTML (1 KB)  PDF (6270 KB)  ( 739 ) 12 The applied value of the BCR technique in diagnosis for diabetic neurogenic bladder and in the evaluation of the efficacy of drugs NIU Xiaoting, SHAO Bei, WANG Xun, ZHANG Yunyun, NI Peiqi, HUANG Huanjie  Objective: To explore the diagnostic value of bulbocavernosus reflex (BCR) in diabetic neurogenic bladder. Methods: Neuroelectrophysiological technique was adopted to determine BCR in 95 diabetes patients with or without sphincter dysfunction (Case Group) and 80 healthy adult (Control Group). All the cases took methylcobalamin tablets for 3 months, then reassed the clinical symptoms, residual urine, blood sugar checks and BCR. Results: BCR latencies in case group were remarkably prolonged than that in control group (P<0.05). BCR amplitudes in case group were remarkably decreased than that in control group (P<0.05). There is no statistical difference of BCR latencies and amplitudes between the two groups before and after methylcobalamin treatment (P>0.05). Conclusion: The patients with diabetic neurogenic bladder have pudendal nerve diseases. BCR is valuable in the topical diagnosis of patients with diabetic neurogenic bladder. Diabetic neurogenic bladder pathological changes are mainly in axonal lesions. The recovery is slow or irreversible after drug treatment. 2014 Vol. 44 (1): 12-14 [Abstract] ( 669 ) HTML (1 KB)  PDF (653 KB)  ( 689 ) 15 The blocker of chloride channels attenuated hypoxic hypercapnia pulmonary vasoconstriction through inhibiting MAPK signal pathway in rats HUANG Linjing1, WANG Shujun2, HE Jinbo1, MA Yingchun1, YING Lei1, CHEN Xiwen3, WANG Wantie1 Objective: To investigate the effect of chloride channels and MAPK signal pathway in the pathological process of hypoxia hypercapnia-induced pulmonary vasoconstriction in rats. Methods: The model of hypoxia hypercapnia-induced pulmonary vasoconstriction rats was used, and the second branch pulmonary artery rings were randomly divided into: control group (N group), hypoxia hypercapnia group (H group), DMSO incubation group (HD group), niflumic acid incubation group (NFA group), niflumic acid+SB203580 incubation group(NFA+SB group), niflumic acid+U0126 incubation group (NFA+U group),  the values of rings’ tension change were recorded via the method of hypoxia hypercapnia conditions reactivity. Results: The second pulmonary artery rings incubated by NFA+SB and NFA+U group who’s phase II persistent vasoconstrictive peak were significantly attenuated and then turn to vasodilatation compared with the HD group (P<0.05 and P<0.01) but the Phase I acute vasoconstriction and the Phase I vasodilation had no changes compared with HD (P>0.05); The second pulmonary artery rings incubated by NFA+SB group who’s vasoconstrictive peak was significantly attenuated compared with the NFA group (P<0.05), but the NFA+U group didn’t change (P>0.05). Conclusion: Chloride channel blocker can inhibit MAPK signaling pathway, reduces the second branch pulmonary artery rings’ phase II sustained contraction, and reversed diastolic state, so as to play the role of HHPV antagonists. 2014 Vol. 44 (1): 15-19 [Abstract] ( 634 ) HTML (1 KB)  PDF (12473 KB)  ( 681 ) 20 Research about upper airway structure of snoring children with lateral X ray film XUAN Miaoyan1, WANG Hongxia1, LI Ting1, PAN Liping1, BAI Guanghui2, CAI Xiaohong1 Objective: To investigate upper airway structure of snoring children through measuring upper airway and surrounding structures of the primary snoring (PS) and obstructive sleep apnea-hypopnea syndrome (OSAHS) children on the lateral X ray film. Methods: The children who came to this hospital because of snoring from October 2008 to December 2010 were be selected. According to the results of polysommography, the children were divided into PS group (53 cases), mild OSAHS group (51 cases), moderate and severe OSAHS group (57 cases). 50 cases unsnoring children were be selected as control group. The four groups were examined with lateral X ray film, and the craniofacial hard tissues, soft tissues, upper airway and space structure were measured. Results: Bone sex structures measured: the hyoid-mandible plane distance (AH-MP), hyoid-frankfort horizontal plane distance (AH-FH), hyoid-precervical vertebra plane distance (AH-CVP)of the three snoring groups obviously increased as compared with the normal group (P<0.05). There was no statistical differences among three snoring groups. Soft tissues measured: the adenoid area (AA) of snoring groups obviously increased as compared with the normal group (P<0.05). The moderate and severe OSAHS group was the maximum, and there was nostatistical differences between PS group and mild OSAHS group. The adenoid thickness (AT), soft palate length (SPL), soft palate thickness (SPT), and soft palate area (SPA) of snoring groups obviously increased as compared with the normal group (P<0.05). There was no statistical differences among snoring groups. Upper airway measured: the posterior airway interstitial (PAS), nasopharyngeal airway area (NA), and nasopharyngeal airway anteroposterior diameter (PNS- UPW) of snoring groups obviously decreased as compared with the normal group (P<0.05). The moderate and severe OSAHS group were the least, and there was no statistical differences between PS group and mild OSAHS group. The palatopharyngeal airway area (PA) and posterior soft palate- posterior soft palate pharynxwall distance (namely palatopharyngeal airway anteroposterior diameter, SPP-SPPW) of snoring groups obviously decreased as compared with the normal group (P<0.05). There was no statistical differences among snoring groups. Space structures measured: the adenoidal - nasopharyngeal ratio (A/N) and adenoidal area-nasopharyngeal area ratio (AA/NTA) of snoring groups obviously increased as compared with the normal group (P<0.05). The moderate and severe OSAHS group was the maximum, and there was no statistical differences between PS group and mild OSAHS group. The PAS-trachea diameter ratio (PAS/TD)of snoring groups obviously decreased as compared with the normal group (P<0.05). The moderate and severe OSAHS group were the least, and there was no statistical differences between PS group and mild OSAHS group. The result of the correlation analysis showed that the correlations between AT, AA, SPA, A/N, AA/NTA and AHI were all positive and significant (P<0.05). The correlations between NA, PNS-UPW, PA, SPP-SPPW, PAS, PAS/TD and AHI were all negative and significant (P<0.05). Conclusion: Adenoidal hypertrophy obstruct the nasopharyngeal airway, the hypertrophy of tonsils and soft palate make the palatopharyngeal and glossopharyngeal airway more serious. Low order and forward hyoid is one of the reasons lead to OSAHS. Lateral X ray film is used to understand upper airway and surrounding structure, and it has important value in research of upper airway structure in snoring children. 2014 Vol. 44 (1): 20-26 [Abstract] ( 837 ) HTML (1 KB)  PDF (8226 KB)  ( 626 ) 27 Analysis on reliability and validity of the Chinese version of the Pediatric Quality of Life InventoryTM Family Impact Module: Supplemental psychometric data in asthma   CHEN Piaopiao1, JIA Xiaohui2, HU Jiao1, ZHANG Liping3, LI Yingchun2. Objective: To test the validity and reliability of C-PedsQLTM FIM in asthmatic children抯 parents, and then provide an effective tool to measure the astnma抯 influence in parents  quality of life and family functioning.Methods: Investigation of the scale was conducted in 165 caregivers of asthmatic children, C-CACT was used to test patients  asthma control condition, and the data were used to evaluate the scale抯 reliability and validity. Results: Seven factors was extracted from C-PedsQLTM FIM by the method of varimax orthogonal rotation of principal component analysis. The contribution rate of each factor was 13.237% to 6.231% and the cumulative contribution rate was 70.603%. The correlation coefficient between C-PedsQLTM FIM and C-CACT was 0.425 (P<0.01), which for each factor was 0.208～0.477 (P<0.01). The coefficient of Cronbach抯αin C-PedsQLTM FIM was 0.960, which for each factor was ranged from 0.830 to 0.932. Conclusion: The reliability and validity of C-PedsQLTM FIM are good enough for meeting psychometric standard. It抯 suitable to assess the asthma抯 influence in caregivers and their family in China. 2014 Vol. 44 (1): 27-30 [Abstract] ( 717 ) HTML (1 KB)  PDF (684 KB)  ( 807 ) 31 Diagnosis of Hirschsprung disease with modified NADPH-d and AchE enzyme histochemistry ZHU Libin, ZHANG Hua, LIN Jinhan, DAI Lina, HUANG Huang, LI Zhongrong Objective: To assess the superiorities of modified NADPH-diaphorase (NADPH-d) and acetylcholinesterase (AchE) enzyme histochemistry staining technique to diagnose Hirschsprung disease (HD). To increase the identification of ganglion cells in the boundary of the bowel in HD and decrease the risks of reoperation. Methods: Full-thickness colon samples of 18 children with HD (girl 7 cases, boy 11 cases ), with average age (8.6±3) months, were examined. Frozen sections at three levels (narrow segment, transitional segment and dilated segment) were performed and investigated to determine the extent of aganglionosis by modified NADPH-d and AchE staining respectively, which were compared by traditional HE staining. TUJ-1 (β-III Tubulin) and PGP9.5(Protein Gene Product 9.5), markers of enteric neural cells, were used for identification of positive myenteric plexus and ganglion cells by immunofluorescence or immunohistochemistry. Results: NADPH-d staining could rapidly confirm whether the normal ganglion cells existed at the proximal end of the resected bowel. AchE technique clearly stained submucosal hypertrophic nerve trucks in aganglion segments. The specifica identifications of HD, NADPH-d staining was 94.4% (17/18), and AchE staining was 88.9% (16/18), respectively (P>0.05). Time comsuming was (7.0±1.7) min in NADPH-d, and (30.9±2.2) min in AchE, respectively (P<0.01). The results of two modified methods mentioned above were in keeping with TUG-landPGP 9.5. Conclusion: Modified NADPH-d staining can rapidly confirmed whether the ganglion cells distribute at proximal end of resected bowel and it is suitable for rapidly intraoperative diagnosis of HD. AchE staining is the best technique to demonstrate hypertrophic nerve trunks in the submucosa and is suitable for diagnosing samples of suction rectal biopsy. 2014 Vol. 44 (1): 31-34 [Abstract] ( 692 ) HTML (1 KB)  PDF (6781 KB)  ( 892 ) 35 Change of serum adiponectin, its influence factors and significance in chronic kidney disease patients  CHEN Huile1, XU Changlong2, XU Xiaojie3, ZHOU Zhihong1 Objective: To explore the changes of the level of serum adiponectin (ADPN) in chronic kidney disease (CKD) patients in different periods, its influence factors and significance. Methods: One hundred and twelve patients with CKD were divided into five groups: 1, 2, 3, 4, 5 stages of CKD groups and 30 normal healthy persons as control group . To investigate serum creatinine (Scr), glomerular filtration rate (GFR). Serum ADPN, high-sensitivity C-reactive protein (hs-CRP), tumor necrosis factor-α(TNF-α), interleukin-6 (IL-6) were measured by Enzyme-linked immunoabsorbent assay (ELISA) and methyl dialdehyde (MDA) was examined with sulfo-barbituric acid. Results: Serum ADPN, hs-CRP, TNF-α, IL-6 and MDA in patients from CKD1 stage to CKD5 stage were significant higher than that in control group (P<0.05 or P<0.01), moreover, all index were increased step by step, existed significant difference between border upon groups (P<0.05 or P<0.01). There were significant positively correlations between the ADPN and hs-CRP (r=0.506), TNF-α(r=0.522), IL-6 (r=0.384), and MDA (r=0.437) (all P<0.01). Conclusion: With the progress of state of an illness of CKD patients, serum ADPN level remarkably gradually elevates. Among micro-inflammation, oxidative stress and decline of renal function induce each other, cooperation may be main causes of change of serum adiponectin. 2014 Vol. 44 (1): 35-38 [Abstract] ( 676 ) HTML (1 KB)  PDF (721 KB)  ( 1035 ) 39 Meta-analysis of the efficacy and safety of mirtazapine versus paroxetine for depression in Chinese patients CHEN Keyang, HUANG Hanjin, WANG Xiaotong.  Objective: To assess the efficacy and safety of mirtazapine and paroxetine on depression in Chinese patients. Methods: The randomized controlled trials (RCT) comparing the efficacy of mirtazapine and paroxetine in the treatment of depression were systematically evaluated. Evidence-based medicine research methods were applied and statistical analysis software Revman 5.2 was used. Results: Fourteen studies of RCT met inclusion criteria. The results of Meta-analysis showed that: ①After 6-weeks treatment, both the efficiency and cure rates had no significant difference between the mirtazapine and the paroxetine groups with [OR=1.15, 95% CI (0.79, 1.68), P=0.46] and [OR=1.17, 95% CI (0.86, 1.58), P=0.24]. The Hamilton Depression Scale (HAMD) scores were significantly lower in mirtazapine  group than that in paroxetine group at 1 and 2 weeks after treatment, and there were no significant differences between mirtazapine group and paroxetine group at 4 and 6 weeks after treatment. ②Adverse reactions: The somnolence and weight gain rate of the mirtazapine group were higher than those of the paroxetine group with RR=2.40 and 95% CI 1.33 to 4.32, RR=10.06 and 95% CI 4.55 to 22.22. And paroxetine more easily led to dry mouth, nausea, insomnia and sexual dysfunction with RR=0.45 and 95% CI 0.31 to 0.67; RR=0.07 and 95% CI 0.03 to 0.17; RR=0.12 and 95% CI 0.04 to 0.34; RR=0.11 and 95% CI 0.04 to 0.27 than mirtazapine. The other common side reactions had no significantly different between the two groups. Conclusion: Mirtazapine has similar long term treatment effect as paroxetine, but it has a rapid-action profile. Mirtazapine can more easily induce somnolence and weight gain, and is with lower dry mouth, nausea, insomnia and sexual dysfunction rate when compared with paroxetine. And there is no significant difference between two group in other common side reactions. 2014 Vol. 44 (1): 39-43 [Abstract] ( 816 ) HTML (1 KB)  PDF (2114 KB)  ( 829 ) 44 Analysis of the related factors between the nonvalvular atrial fibrillation and the cerebral infarction  ZHENG Jianlei1, YAN Peiyuan2, WANG Haiqing1 Objective: To explore the relationship among the mean platelet volume, fibrinogen and left atrial size and cerebral infarction in patients with nonvalvular atrial fibrillation (AF). Methods: One hundred and sixty-five patients with AF were divided into AF with and without cerebral infarction diagnosed with computed tomography (CT) and/or magnetic resonance imaging (MRI). CHA2DS2-vasc score, mean platelet volume, plasma fibrinogen, left atrial size, blood pressure, serum lipid level and renal function were detected in the enrolled population. Results: Compared with the patients without cerebral infarction, the AF patients with cerebral infarction showed higher level of CHA2DS2-vasc score (P<0.001), plasma fibrinogen (P=0.002), mean platelet volume (P<0.001), fasting plasma glucose (P=0.034), lipoprotein (a) (P=0.017) and larger left atrial size (P<0.001). After adjustment of multiple traditional cardiovascular risk factors, logistic regression analysis showed that mean platelet volume, plasma fibrinogen, left atrial size and CHA2DS2-vasc score were independent risk factors for AF-related cerebral infarction. Conclusion: Except CHA2DS2-vasc score, the combined index of mean platelet volume, plasma fibrinogen and left atrial size may also play an important role in predicting the occurrence of cerebral infarction in patients with AF. 2014 Vol. 44 (1): 44-47 [Abstract] ( 685 ) HTML (1 KB)  PDF (678 KB)  ( 774 ) 48 Analysis of modified Alvarado scoring system and its correlation with pathological grades of acute appendicitis in infants HOU Liangchao, LIN Xiaokun, LV Min, ZHANG Haochuan, CHEN Xiaoming Objective: To explore the application of the modified Alvarado scoring system in infant acute appendicitis, and provide the diagnostic basis to infant appendicitis. Methods: The clinical data of 120 cases of infants who had undergone appendectomy form 2008 to 2013 in the Department of Pediatric Surgery of Yuying Children’s Hospital Affiliated to Wenzhou Medical University were analyzed retrospectively. We used paroxysmal crying abdominal pain and bloating instead of migration of pain and right lower quadrant tenderness, and analyzed the results of Alvarado scoring system with pathological diagnosis. Results: The scores of 120 cases of infants correlated positively with pathological grades (r=0.39, P<0.01). And the sensitivity and accuracy of the Alvarado scoring system were 96.5% and 92.5% respectively, when we used the score for predicting infants acute appendicitis  with the advanced pathological grades. Conclusion: It shows that the modified Alvarado score system is mainly aimed at the clinical manifestations, including medical history and physical examination, etc. And it is useful in infant appendicitis for improving diagnosis accuracy and reducing the risks of perforation. 2014 Vol. 44 (1): 48-50 [Abstract] ( 673 ) HTML (1 KB)  PDF (673 KB)  ( 952 ) 51 Analysis the effect of traditional Chinese combined with western medicine in treatment of pharyngitis caused by laryngopharyngeal reflux WU Xianmin1, SHANGGUAN Hanjing2, CHEN Xiaoyun1, ZHANG Yue1, LI He1, CHEN Jianfu1  Objective: To investigate the effect of Chinese traditional combined with Western medicine in treatment of pharyngitis caused by laryngopharyngeal reflux. Methods: Retrospectively analysis of treatment for throat discomfort, reflux symptom index (RSI) score >13 points and (or) reflux finding score (RFS) >7 points were positive, of which the score of RSF positive was 100 cases, the score of RSI positive 105 cases, combined with laryngoscope examination and clinical manifestations, 105 cases were diagnosed as LPR. 105 cases were Randomly divided into three groups. the treatment method included avoiding high fat, high sugar, hot and spicy diet and changing lifestyle (such as smoking, alcohol and  no longer eat after 9 pm). 35 cases in A group were treated with omeprazole [proton pump inhibitor (PPI)], domperidone and jingshen moisten throat agent therapy, 35 cases of B group were treated with omeprazole and domperidone therapy, 35 cases of C group were treated with omeprazole therapy. The curative effect was observed after the end of treatment for 3 months. Results: In group A, 25 cases (71.43%) were markedly effective, 7 cases (20%) were effective, 3 cases (8.57%) were invalid, the total effective rate was 91.43%; in group B, 17 cases (48.57%) were markedly effective, 5 cases (14.29%) were effective, 13 cases (37.14%) were invalid, the total effective rate was 62.86%; in group C, 12 cases (34.29%) were markedly effective, 6 cases (17.14%) were effective, 17 cases (48.57%) were invalid, the total effective rate was 51.43%. The difference was statistically significant (x2=13.788, P<0.05) when comparison of the total efficiency among the three groups. Comparison between the two groups: the total effective rate of A group was higher than that of B group, and the difference was statistically significant (x2=6.563, P<0.0125), the total effective rate of A group was higher than that of C group, and the difference was statistically significant (x2=11.830, P<0.0125). The total effective rate between B group and C group were no significant difference, and the difference had no statistically significant (x2=0.933, P>0.0125). Main symptom remission time (weeks) in throat department in the A group was shorter than that in the B group, also shorter than that in the C group, main symptom remission time in the B group in throat department  was shorter than that in the C group, the differences were statistically significant (all P<0.05). Conclusion: Traditional Chinese combined with Western medicine had better clinical effect in the treatment of pharyngitis caused by LPR. 2014 Vol. 44 (1): 51-54 [Abstract] ( 722 ) HTML (1 KB)  PDF (729 KB)  ( 760 ) 55 Analysis on the causes of children death in the Yuying Children Hospital Affiliated to Wenzhou Medical University from 2000 to 2011 XIA Tianhe1, WANG Zhenquan1, ZHAGN Yuanhai1, XIE Zuokai2  Objective: To understand the causes of death, distribution of hospitalized children sex and age  from 2000 to 2011. Methods: A retrospective statistical analysis was performed in 539 death cases among hospitalized children from 2000 to 2011. The results were analysed on SPSS 17.0 software. Results: There were 539 cases of death among hospitalized children from 2000 to 2011, the ratio of boys/girls was 2.12:1, and the ratio of countryside/city was 1.72:1. The propotion of death cases under 1 year group was the most. The cause of death of all ages was different. The top three causes of neonates death were: congenital malformations, hyaline membrane disease and neonatal asphyxia. The top three causes of death in 29 d~1 year group were: congenital heart disease, pneumonia and sepsis. In 1~3 year group were: congenital heart disease, accidental poisoning injury and infectious diseases. In 4~7 year group were: accidental injury, congenital heart disease and sepsis. In 8~15 year group were: cancer, accidental injury and congenital heart disease. Conclution: The key to reduce child mortality is to strengthen perinatal care, health education of parents, and enhance safety awareness, prevention and control of environmental pollution, prevent infectious diseases to decrease the mortality. 2014 Vol. 44 (1): 55-58 [Abstract] ( 750 ) HTML (1 KB)  PDF (715 KB)  ( 978 ) 58 Assessment of left ventricular regional wall motion with 640-slice MDCT: a comparison with 2D-echocardiography PAN Kehua, CAO Guoquan, SUN Houzhang, XU Huazhi, WANG Aimin Objective: To evaluate the value of 640-slice MDCT in assessment of left ventricular regional wall motion (LVRWM), using 2D-echocardiography as the reference standard. Methods: Sixty-two patients undergone 640-slice MDCT and 2D-echocardiography were evaluate. The motion of 1054 segments was visually assessed by two radiologists with a 3-piont scale using a 17-segment model. A separate reader assessedLVRWM of 1054 segments on 2D-echocardiography using the same criteria as did in the MDCT. Results: LVRWM abnormalities were detected in 196 and 190 segments on MDCT and 2D-echocardiography respectively, of which 58 and 50 segments were hypokinesis or akinesis, 138 and 140 segments were mild or moderate hypokinesis. The sensitivity, specificity and accuracy of MDCT for diagnosing abnormal segment wall motion were 94.0%, 77.0%, and 91.0% respectively. The data of wall motion and wall thickening of each LV segment were also acquired. Conclusion: The value of 640-slice MDCT in qualitatively assessment of LVRWM is significant, and it shows great potential in quantitatively assessment of LVRWM. 2014 Vol. 44 (1): 58-61 [Abstract] ( 628 ) HTML (1 KB)  PDF (2145 KB)  ( 617 )

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