mtND5基因12338、12358和12406位点突变与弱精子症的相关性分析#br#

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Abstract Objective: To investigate the correlation between asthenospermia and the mutation site of 12338, 12358 and 12406 in mtND5. Methods: Fifty-five semen samples from patients with asthenospermia and 33 from healthy donors based on WHO criteria were collected and analyzed, mutation frequency of 12338, 12358 and 12406 in mtND5 was examined by polymerase chain reaction (PCR) and sequencing. Results: The mutation frequency (m.12338 T>C 10.9%, m.12358 A>G 9.09%, m.12406G>A 12.7%, total 32.73%) in asthenospermia group was higher than the frequency in control group (9.09%, 3.03%, 3.03% and 15.15%), but there was no significant difference between them (P>0.05). However, the percentages of grade a (20.63±13.63) and grade (a+b) (29.66±17.08) sperm in mutation samples (with m.12338 T>C or m.12358 A>G or m.12406 G>A) were significantly lower than that in non-mutation samples (30.61±18.87 and 41.44±22.47) (P<0.05). Conclusion: These mutations of m.12338 T>C, m.12358 A>G and m.12406 G>A in human sperm may have some correlation with sperm motility, but may not be the specific mutation site of asthenospermia.

Key words： asthenospermia sperm motility mtND5 mutation

Received: 26 November 2013

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	LUO Huiying1
	LI Chuanlian2
	LOU Zhefeng1
	ZHENG Jiujia3
	ZHANG Liya3
	JIN Longjin1.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2014/V44/I3/169

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