长链非编码RNA CasC7对宫颈癌细胞增殖的影响

Abstract
Figure/Table
References (0)
Related Citation (15)

Download: PDF (1505 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To investigate the expression level of lncRNA CasC7 in cervical cancer and its role in tumorigenesis and development of cervical cancer. Methods: Bioinformatics was utilized to analyse the expression level of lncRNA CasC7 in GSE9750, a gene expression microarrays for cervical cancer. Quantitative real-time PCR (qRT-PCR) was used to detect the expression level of CasC7 in cervical cancer tissues, cervical intraepithelial neoplasia tissues, normal cervical tissues and normal cervical epithelial cells (H8 cells), cervical cancer cells (SiHa cells, CaSki cells and HeLa cells). Association between the expression of CasC7 and clinicopathological features of cervical cancer was analyzed. Meanwhile, the CCK-8 assays was used to measured cell proliferation when CasC7 expression was inhibited. Results: LncRNA expression in cervical cancer and cervical intraepithelial neoplasia was higher than that in normal cervical tissues (P<0.01). Compared with normal cervical epithelial cells, CasC7 was up-regulated in SiHa cells, CaSki cells and HeLa cells (P<0.05). High expression of CasC7 was correlated with advanced FIGO stage (P=0.001) and poor differentiation (P=0.015). However, neither patients age nor tumor size was associated with the expression of CasC7 (P>0.05). CCK-8 assays showed that cell proliferation of SiHa cells, CaSki cells and HeLa cells was suppressed by CasC7 knockdown (P<0.05). Conclusion: CasC7 may act as an oncogene during the development of cervical cancer.

Key words： long noncoding RNA CasC7 uterine cervical neoplasms cell proliferation

Received: 11 March 2017

	Service
	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	AN Dewen
	HU Yingying
	LIANG Zongwen
	XIANG Junmiao
	YU Danyang
	DUAN Ping

URL:

https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I2/91

[1] XING Z, PARK P K, LIN C, et al. LncRNA BCAR4 wires up signaling transduction in breast cancer[J]. RNA Biol, 2015, 12(7): 681-689.
[2] IGUCHI T, UCHI R, NAMBARA S, et al. A long noncoding RNA, lncRNA-ATB, is involved in the progression and prognosis of colorectal cancer[J]. Anticancer Res, 2015, 35(3): 1385-1388.
[3] ZHUANG J, LU Q, SHEN B, et al. TGFβ1 secreted by cancer-associated fibroblasts induces epithelial-mesenchymal transition of bladder cancer cells through lncRNA-ZEB-2NAT[J]. Sci Rep, 2015, 5: 11924.
[4] ARASE M, HORIGUCHI K, EHATA S, et al. Transforming growth factor-β-induced lncRNA-Smad7 inhibits apoptosis of mouse breast cancer JygMC (A) cells[J]. Cancer Sci, 2014, 105(8): 974-982.
[5] SCHMITT A M, CHANG H Y. Long noncoding RNAs in cancer pathways[J]. Cancer Cell, 2016, 29(4): 452-463.
[6] YLIPää A, KIVINUMMI K, KOHVAKKA A, et al. Transcriptome sequencing reveals PCAT5 as a novel ERG-regulated long noncoding RNA in prostate cancer[J]. Cancer Res, 2015, 75(19): 4026-4031.
[7] WANG Y, HE L, DU Y, et al. The long noncoding RNA lncTCF7 promotes self-renewal of human liver cancer stem cells through activation of Wnt signaling[J]. Cell Stem Cell, 2015, 16(4): 413-425.
[8] 孙祥威, 胡盈盈, 徐剑峰, 等. 长链非编码RNA AL049452在结直肠癌组织中的表达及临床意义[J]. 温州医科大学学报, 2017, 47(8): 566-570.
[9] QIU M, XU Y, WANG J, et al. A novel lncRNA, LUADT1, promotes lung adenocarcinoma proliferation via the epigenetic suppression of p27[J]. Cell Death Dis, 2015, 6: e1858.
[10] ZHANG S, ZHANG G, LIU J. Long noncoding RNA PVT1 promotes cervical cancer progression through epigenetically silencing miR-200b[J]. APMIS, 2016, 124(8): 649-658.
[11] YACQUB-USMAN K, PICKARD M R, WILLIAMS G T. Reciprocal regulation of GAS5 lncRNA levels and mTOR inhibitor action in prostate cancer cells[J]. Prostate, 2015, 75 (7): 693-705.
[12] GUTSCHNER T, HäMMERLE M, EISSMANN M, et al. The noncoding RNA MALAT1 is a critical regulator of the metastasis phenotype of lung cancer cells[J]. Cancer Res, 2013, 73(3): 1180-1189.
[13] TAN J Y, VANCE K W, VARELA M A, et al. Cross-talking noncoding RNAs contribute to cell-specific neurodegeneration in SCA7[J]. Nat Struct Mol Biol, 2014, 21(11): 955-961.
[14] TAJIRI N, ACOSTA S A, SHAHADUZZAMAN M, et al. Intravenous transplants of human adipose-derived stem cell protect the brain from traumatic brain injury-induced neurodegeneration and motor and cognitive impairments: cell graft biodistribution and soluble factors in young and aged rats[J]. J Neurosci, 2014, 34(1): 313-326.
[15] ZHANG J, YAO T, WANG Y, et al. Long noncoding RNA MEG3 is downregulated in cervical cancer and affects cell proliferation and apoptosis by regulating miR-21[J]. Cancer Biol Ther, 2016, 17(1): 104-113.
[16] SUN R, QIN C, JIANG B, et al. Down-regulation of MALAT1 inhibits cervical cancer cell invasion and metastasis by inhibition of epithelial-mesenchymal transition[J]. Mol Biosyst, 2016, 12(3): 952-962.
[17] ZHANG D, ZHOU X H, ZHANG J, et al. Propofol promotes cell apoptosis via inhibiting HOTAIR mediated mTOR pathway in cervical cancer[J]. Biochem Biophys Res Commun, 2015, 468(4): 561-567.
[18] JIN X, CHEN X, HU Y, et al. LncRNA-TCONS_00026907 is involved in the progression and prognosis of cervical cancer through inhibiting miR-143-5p[J]. Cancer Med, 2017, 6 (6): 1409-1423.
[19] ZHANG D, SUN G, ZHANG H, et al. Long non-coding RNA ANRIL indicates a poor prognosis of cervical cancer and promotes carcinogenesis via PI3K/Akt pathways[J]. Biomed Pharmacother, 2017, 85: 511-516.
[20] KIM H J, EOH K J, KIM L K, et al. The long noncoding RNA HOXA11 antisense induces tumor progression and stemness maintenance in cervical cancer[J]. Oncotarget, 2016, 7(50): 83001-83016.
[21] LENNOX K A,BEHLKE M A. Cellular localization of long non-coding RNAs affects silencing by RNAi more than by antisense oligonucleotides[J].Nucleic Acids Res, 2016, 44 (2): 863-877.