神经肌肉电刺激对低O2高CO2大鼠下肢骨骼肌miR-1相关通路的影响

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Abstract Objective: To explore the effects of neuromuscular electrical stimulation on gastrocnemius muscle of chronic intermittent hypoxia-hypercapnia (CIHH) rats and try to find out how much the miR-1 related signaling pathway is involved in. Methods: Twenty four male SD rats were randomly divided into three groups: the normal control group (NC), hypoxia-hypercapnia group (HH), hypoxia-hypercapnia+electrical stimulation group (HE). The HH and HE were placed in a chamber with hypoxia-hypercapnia for 8 h/d, 4 weeks in all. After 2 weeks, 30 min, 2 Hz and 100 Hz, 1:1 cyclic alternating pattern of electrical stimulation was applied in the HE, which lasted 2 weeks. The NC was only tied up and placed with electrode plates. The endurance running capacity was assessed by the treadmill. The muscle phenotype was observed by immunohistochemical staining. The expression of miR-1 by qRT-PCR was detected. The expression of HDAC4 (histone deacetylase 4), MEF2 (myocyte enhancer factor 2) and PGC-1α were detected by Western blot. Results: After 4 weeks, the HH group exhibited a decreased MHCI-to-MHCII shift (P<0.05). Nuclear HDAC4, MEF2 and PGC-1α was also significantly decreased (P<0.05). In contrast, miR-1 was significantly increased (P<0.05). Electrical stimulation can partly reversed those changes, and improved endurance running capacity. Conclusion: Our study indicate the miR-1 related signaling pathway may play a role in the response of CIHH-impaired muscle to changes during electrical stimulation.

Key words： chronic intermittent hypoxia-hypercapnia chronic obstructive pulmonary disease neuromu-scular electrical stimulation histone deacetylase 4 miR-1 rats

Received: 09 October 2017

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	PAN Lulu
	ZHI Yinghao
	WANG Xiaotong

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I2/86

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