土忍翘薇合剂对大鼠CYP450酶活力和mRNA的调控作用

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Abstract Objective: To investigate the modulation of TuRenQiaoWei decoction on activities and mRNA expressions of cytochrome P450 (CYP450) enzymes in rats. Methods: Twenty SD rats were randomly divided into treatment group and control group. After treatment with TuRenQiaoWei decoction or water for 14 days, treatment group rats and control group rats were given the mixture of four probe substrates including phenacetin, bupropion, chlorzoxazone and midazolam. The plasma concentration of probes at a series of time-points were determined by liquid chromatography tandom mass spectrometry. The activities of CYP450s were evaluated according to the pharmacokinetic parameters of corresponding substrates. Real-time PCR was applied to assess the mRNA expression levels of CYP450s. Results: The pharmacokinetic parameters of phenacetin and midazolam from treatment group showed significant differences compared with control group, which indicated that TuRenQiaoWei decoction induced CYP1A2 and CYP3A1 activity. And no significant difference was found in pharmacokinetic parameters of bupropion or chlorzoxazone from treatment group and control group. In addition, treatment with TuRenQiaoWei decoction significantly up-regulated the mRNA expression of CYP1A2 and CYP3A1 whereas it had no impact on CYP2B1 and CYP2E1. Conclusion: TuRenQiaoWei decoction can up-regulate the activities and mRNA expressions of CYP1A2 and CYP3A1.

Key words： TuRenQiaoWei decoction cytochrome P-450 enzyme system activity RNA, messenger

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	HUANG Aifang
	WANG Chenxiang
	ZHOU Xiaojie
	JIN Hui
	ZHOU Bin
	YU Xuben
	LIN Guanyang

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2018/V48/I1/57

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