慢性低O2高CO2对小鼠海马细胞凋亡、caspase-3和p38 MAPK活性的影响

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Abstract Objective: To investigate the effect of the chronic hypoxic-hypercapnia in apoptosis and activity of p38 MAPK and caspase-3 of hippocampus in mouse. Methods: Twenty-four male mice were randomly divided into two groups: the hypoxic-hypercapnia 4 weeks (4HH) group and normal control (NC) group. The 4HH groups in the special cantainer were exposed to an atmosphere containing 9%～11% O2 and 5%～6% CO2 8 hours a day, 6 days a week for 4 weeks. The two groups lived in the same room in the rest time. Apoptosis index (AI) in hippocampus was counted by TUNEL staining. Caspase-3 activity was measured by a caspase-3 activity assay kit. p-p38 MAPK protein in hippocampus was measured by Western blot. Results: Compared with NC group the AI, caspase-3 activity, and the p-p38 MAPK protein expression in hippocampus were significantly increased in 4HH group (P<0.01). Conclusion: The chronic hypoxic-hypercapnia promotes apoptosis and the activation of caspase-3 and p38 MAPK of hippocampus in mouse.

Key words： hypoxia, hypercapnia p38 mitogen-activated protein kinases caspase-3 apoptosis mouse hippocampus

Received: 30 March 2017

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	REN Huiming
	WANG Xiaotong
	YUAN Hai
	KANG Hanlin.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I12/895

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