树脂改性玻璃离子Vitrebond的光固化时间对细胞毒性的影响

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Abstract Objective: To investigate the influence of curing time of curing light on the cytotoxicity of Vitrebond, and compare the cytotoxic sensitivity of Agar diffusion test and MTT assay. Methods: Vitrebond of 2 mm thickness were light-cured for 15, 30, 45 seconds with 1 000 mw/cm2 curing light. The effects of different curing time of Vitrebond on L929 cell were evaluated by Agar diffusion test and MTT assay after 24 h and 48 h. Results: The evaluation of agar diffusion found the positive control and the specimen without any curing induced severe cytotoxity, there was no cytotoxicity in negative control, any of specimens and any of MEM or cottonseed oil extracts. In MTT test, Vitrebond showed a great cytotoxicity whatever the curing time was. There was no statistical difference of cytotoxicity cured for 15, 30, and 45 seconds (F=1.452, P>0.05). Conclusion: There are no difference of cytotoxicity of Vitrebond cured with 1 000 mw/cm2, with different curing time. Agar diffusion test is lower sensitive than MTT test to detect the cytotoxicity.

Key words： resin-modified glass-ionomer cements cytotoxicity light curing vitrebond agar diffusion MTT

Received: 11 May 2017

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	DU Ruoxi
	ZHOU Qiaozhen
	YANG Zhengyu
	MA Jianfeng.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I12/875

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