维生素B2修饰的多壁碳纳米管电极上电化学方法测定DNA

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Abstract Objective: The research on interaction between vitamin B2 which was on the vitamin B2/multiwall carbon nanotube modified electrode and DNA by electrochemistry and UV spectra methods was performed. Methods: There was an obvious pair of reduction and oxidation peaks on the prepared vitamin B2/multiwall carbon nanotube modified electrode. Both reduction and oxidation peak currents of vitamin B2 decreased with no change in the peak potentials after adding DNA. Through the test of the electrochemical parameters in the absence and presence of DNA, it was found that after combination of vitamin B2 and DNA under such condition, an electrochemically inactive supramolecular compound arised. Results: Under the experimental conditions selected, the decrease of oxidation peak currents had a linear relationship with DNA concentrations in the range of 0.01 to 0.1 mg/mL and 0.1 to 0.5 mg/mL, which could be used for measuring DNA concentration. Conclusion: The developed method is simple, rapid, economical and has good stability and reproducibility.

Received: 22 May 2017

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	ZHANG Tiantian
	YANG Lizhu.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I12/869

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