葛根素对2型糖尿病大鼠胰腺β细胞损伤的影响

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Abstract Objective: To study the effects of puerarin on diabetic pancreatic β-cell injury and to explore its possible mechanisms in type 2 diabetic rats. Methods: A type 2 diabetic rat model was established by the combination of a high-fat diet and a single low-dose streptozocin intraperitoneal injection. The animals were randomly divided into diabetic model group and puerarin (100 mg•kg-1, i.p.) treatment group, with other 8 normal rats as a control group. The rats were treated daily for 4 consecutive weeks, and the equal volume of vehicle (5% propanediol, i.p.) was given to the normal control and diabetic model group. The rats were sacrificed and fasting blood glucose, glycated hemoglobin (HbA1c) and serum insulin levels, as well as malondialdehyde (MDA) content, the activities of superoxide dismutase (SOD) and catalase (CAT) in pancreas were measured. The pancreatic telomere length was determined by real-time quantitative PCR. The protein levels of phosphorylated AMP-activated protein kinase (AMPK), sirtuin1 (SIRT1) and uncoupling protein 2 (UCP2) as well as apoptosis-related proteins, e.g. cleaved caspase-3/caspase-3, Bim, Bax and Bcl-2 in pancreas were assessed by Western blot. Results: The fasting blood glucose, HbA1c, and MDA contents as well as the protein expressions of UCP2, Bax, Bim and cleaved caspase-3/caspase-3 in pancreas of diabetic rats were significantly higher than those in normal controls (P<0.01, respectively). However, insulin level in serum, SOD and CAT activities, telomere length as well as the protein expressions of p-AMPK/AMPK, SIRT1 and Bcl-2 in pancreas of diabetic rats were significantly decreased as compared with those in normal animals (P<0.01, respectively). In contrast, in puerarin treatment group, the above changes were reversed, significant differences of those were found as compared with those in diabetic model group (P<0.01, respectively). Conclusion: Puerarin exerts preventive and remedial effects on the diabetic pancreatic β-cell, which is probably due to protecting telomere length and inhibiting β-cell apoptosis via alleviating oxidative damage, and decreasing UCP2 expression through enhancing SIRT1 activity as well.

Key words： puerarin diabetes mellitus pancreas telomere length apoptosis uncoupling protein 2

Received: 08 June 2017

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	CHEN Xiufang
	LEI Kangfu
	DONG Min
	ZHENG Qiaomin

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I12/859

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