香砂六君丸对非酒精性脂肪肝大鼠肝脏线粒体的影响

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Abstract Objective: To observe the effect of Xiangsha Liujunzi Pill on liver’s mitochondrial in rats with nonalcoholic fatty liver disease. Methods: Under specific pathogen free (SPF) conditions, 24 male Sprague-Dawley (SD) rats were randomly divided into control group, high fat and high cholesterol diet group (HFD) and Xiangsha Liujunzi Pill (1.35 g•kg-1•d-1) treatment group. After 12 weeks, all rats were executed to get liver samples. Blood paramaters were analyzed by automatic biochemical. The copy number of mitochondrial DNA (mtDNA) and the activity of mitochondrial respiratory chain complex I, IV in liver samples was analysized with RT-PCR and UV spectrophotometer respectively. Results: Compared with control group, the hepatic wet weight and mass index (hepatic wet weight/body mass) increased significantly, levels of total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in serum were significantly increased, and the copy number of mtDNA and the activity of complex I, IV expression were significantly decreased in HFD group. Compared with HFD group, the liver wet weight and quality index of rats were significantly reduced, levels of TC and LDL-C in serum were significantly increased, and the copy number of mtDNA and the activity of complex I, IV expression were significantly increased in Xiangsha Liujunzi Pill treatment group. The HE staining of liver showed that the livers sections of HFD group were abundant of macrovesicular fat droplets in hepatocytes and large quantities of ballooned hepatocytes in centrilobular parenchyma with infiltration of inflammatory cells and fibrosis. The livers sections of HFD group showed that the macrovesicular fat droplets decreased and the nucleus consolidation was reduced. Conclusion: Xiangsha Liujunzi Pill would has effect on nonalcoholic fatty liver disease. Xiangsha Liujunzi Pill can regulate the disordered mitochondrial respiratory chain complex in nonalcoholic fatty liver disease.

Key words： nonalcoholic fatty liver disease Xiangsha Liujunzi Pill mitochondrial resporatory chain complex DNA, mitochondrial

Received: 22 October 2016

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	Articles by authors
	Lou Linjie1
	You Yanting2
	MA Ke2
	Chen Zewei2
	Sun Xiaomin2
	Zhou Lin3.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I11/796

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