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Effect of gossypol on hepatic fibrosis of nonalcoholic fatty liver disease in diabetic rats |
LI Yangyang1, CHEN Hanbin2, XU Feifei1, WAN Li1, CHEN Guorong1, CHEN Lei3, CHEN Sanmei4, WANG Rongrong1. |
1.Department of Pathology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 2.Department of Radiation and Chemotherapy, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015; 3.The First Clinical Medical College, Wenzhou Medical University, Wenzhou, 325035; 4.Department of Nursing, School of Medical Sciences, Shaoxing University, Shaoxing, 312000 |
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Cite this article: |
LI Yangyang,CHEN Hanbin,XU Feifei, et al. Effect of gossypol on hepatic fibrosis of nonalcoholic fatty liver disease in diabetic rats[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(11): 786-790.
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Abstract Objective: To investigate the effect of gossypol on nonalcoholic fatty liver disease (NAFLD) and its mechanism. Methods: Thirty male Sprague-Dawley rats were divided randomly into 3 groups: normal control (NC) group, type 2 diabetes mellitus (T2DM) group, type 2 diabetes mellitus treated with gossypol (T2DMG) group. After fed with a high-fat diet, the rats were injected with low-dose streptozotocin (STZ) intraperitoneally to induce type 2 diabetes mellitus. The T2DMG group were given 15 mg•kg-1•d-1 gossypol by gavage for 4 weeks, followed by 15 mg•kg-1•d-1 dose of gossypol every week for 8 weeks. The morphology of the liver was observed under light and transmission electronmicroscopy. Masson staining were used to observe the fibrosis of liver tissue. The protein expression of α-SMA, 11β-HSD1, GR, NF-κB and TGFβ1 were assayed by immunohistochemistry. The mRNA expression of 11β-HSD1, GR, NF-κB and TGFβ1 from rats livers were assayed by semi-quantitative RT-PCR. Results: Compare with NC group, T2DM group hapatic fibrosis was obvious, α-SMA, 11β-HSD1, GR, NF-κB and TGFβ1 protein expression was significantly enhanced (P=0.001, 0.004, 0.001, 0.001, 0.002), GR and TGFβ1 mRNA expression was significantly increased (P=0.01, 0.001). Compare with T2DM group, T2DMG group liver fibrosis was significantly ameliorated, α-SMA, 11β-HSD1, GR, NF-κB and TGFβ1 expression was significantly decreased (P=0.009, 0.031, 0.002, 0.009, 0.027), 11β-HSD1, GR and TGFβ1 mRNA expression was significantly reduced (P=0.003, 0.038, 0.014). Conclusion: Gossypol exerts the capacity to relieve hepatic fibrosis from NAFLD, inhibiting the expression of 11β-HSD1, GR and NF-κB, TGFβ1 may be involved in it.
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Received: 24 April 2017
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