MiR-152对转化生长因子β1诱导的气管上皮细胞向间质细胞转化的影响

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Abstract Objective: To verify whether transforming growth factor-beta1 (TGF-β1) could induce epithelial-mesenchymal transition (EMT) in vitro and to determine the role of miR-152 in TGF-β1-mediated EMT in bronchial epithelial cells. Methods: 10 ng/mL of TGF-β1 was used to induce the EMT of human bronchial epithelial cells 16HBE. Morphological changes were observed and qRT-PCR and Western blot were employed to test the alteration of αSMA, E-cadherin and Vimentin, thus to verify the occurrence of EMT. qRT-PCR was then used to test the expression profile of miR-152 in TGF-β1 treated 16HBE cells. After miR-152 was successfully transfected into 16HBE cells, EMT related markers were then detected using qRT-PCR and Western blot. Results: Compared with control group, TGF-β1-treated cells presented a myofibroblast-like morphology, especially at 72 h, characteristic by the loss of cell-to-cell junction, denovo expression of α-SMA and Vimentin, and loss of epithelial marker E-cadherin (P<0.01). The expression levels of miR-152 in TGF-β1 treated 16HBE cells were dramatically increased after transfected with miR-152 mimics (P<0.01). Compared with the control group, α-SMA and Vimentin were decreased, whereas E-cadherin was increased in the miR-152 group (P<0.01), suggesting overexpression of miR-152 could reverse the EMT induced by TGF-β1 in 16HBE cells. Conclusion: TGF-β1 induces EMT of human bronchial epithelial cells in vitro; miR-152 inhibits TGF-β1-mediated EMT in 16HBE cells.

Key words： asthma bronchial epithelial cells mesenchymal cells miR-152

Received: 13 April 2017

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	ZHU Ronghe
	SUN Yuanyuan
	QIAN Yan.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I10/739

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