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| Curcumin inhibits the proliferation of human renal cell carcinoma 786-O in nude mice via Erk1/2 signaling pathway |
| ZHAO Jiantong1, ZHANG Lei2, LIU Wenzhan1, XU Fei1, GUO Mingtao1, LU Zhimin1, XIAO Bo1. |
| 1.Department of Urology, Handan First People’s Hospital, Handan, 056000; 2.Institute of Foundation, Hebei Medical University, Shijiazhuang, 050012 |
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| Cite this article: |
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ZHAO Jiantong,ZHANG Lei,LIU Wenzhan, et al. Curcumin inhibits the proliferation of human renal cell carcinoma 786-O in nude mice via Erk1/2 signaling pathway[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(10): 735-738.
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Abstract Objective: To investigate the inhibitory effect of curcumin on 786-O cells and explore the potential mechanisms. Methods: Nude mice were randomly divided into two groups. Human kidney cancer cells 786-O were inoculated in nude mice. Intervention group mice were treated with curcumin and the control group mice were treated with PBS. The inhibition rate of tumor in the intervention group was determined. p-Erk1/2 and p-Akt expression was detected using Western blot and immunohistochemistry. Results: The average weight of the tumor of nude mice was (1.03±0.17)g in the intervention group and (2.46±0.48)g in the control group, the difference was significant (P=0.02). The tumor inhibition rate of mice treated with curcumin was 58.13%. Western blot and immunohistochemical staining showed that the phosphorylation level of Erk1/2 in intervention group was significantly lower than that in the control group (P<0.01), no significantly difference was observed in the expression of Erk1/2, Akt and p-Akt in the two groups (P>0.05). Conclusion: The results of this study show that curcumin can inhibit the growth of tumor in vivo and inhibit the phosphorylation of Erk1/2 and inhibit the proliferation of human renal carcinoma cell line 786-O in nude mice.
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Received: 18 April 2017
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[1].狄建彬, 顾振纶, 赵笑东, 等. 姜黄素的抗氧化和抗炎作用研究进展[J]. 中草药, 2010, 41(5): 854-859.
[2].陈四梅, 张森凯, 刘网网, 等. 姜黄素衍生物L6H4对糖脂代谢异常大鼠的治疗作用及其机制[J]. 温州医科大学学报, 2015, 45(3): 176-180.
[3].姜程曦, 林良义, 宋娇, 等. 姜黄素类似物抑制炎症反应缓解1型糖尿病肾损伤实验研究[J]. 中草药, 2015, 46(12): 1785-1790.
[4].吴杰, 徐娇娇, 叶娟, 等. 天然姜黄素类化合物抗人类呼吸道合胞病毒研究[J]. 中国药师, 2015, 18(1): 34-37.
[5].郑丹, 韩文文, 洪广亮, 等. 姜黄素对百草枯中毒大鼠急性肾氧化损伤的干预作用[J]. 温州医科大学学报, 2016, 46 (6): 391-396. [1].陈建平, 李琳, 苏健裕. 姜黄素的抗氧化及抗肿瘤活性研究[6]. 现代食品科技, 2014, 30(12): 11-16.
[7].林清认, 周霞. 姜黄素对肺癌细胞的放疗增敏作用及机制研究[J]. 中国生化药物杂志, 2014, 34(5): 33-36.
[8]. 郭立达, 焦振霞, 宋瑛, 等. 姜黄素诱导结肠癌LoVo细胞凋亡的作用及机制研究[J]. 中国中药杂志, 2013, 38(13): 2191-2196.
[9].盛琦, 陈建, 李菌. 姜黄素逆转非小细胞肺癌吉非替尼耐药的研究[J]. 中国现代应用药学, 2013, 30(4): 360-364.
[10].杨芳, 赵秋, 王渝, 等. 姜黄素抑制STAT3信号通路对胰腺癌细胞增殖的影响[J]. 世界华人消化杂志, 2011, 19(30): 3149-3153.
[11].赵建通, 张磊, 刘文瞻, 等. 姜黄素与索拉非尼联合对人肾癌细胞株786-O增殖抑制作用的体外研究[J]. 河北医药, 2016, 38(4): 495-501.
[12].周海生, 张爱伟, 陈伟建, 等. MRI扩散加权成像在乏脂肪肾血管平滑肌脂肪瘤和肾癌间的鉴别诊断价值[J]. 温州医科大学学报, 2016, 46(6): 447-450.
[13].杨淑哲, 罗春丽, 吴小候, 等. hepaCAM和VEGF基因表达与肾透明细胞癌侵袭转移关系的探讨[J]. 生物技术通报, 2010, 16(1): 157-160.
[14].史培堃, 曾贝妮, 吴伟芳, 等. Keap1在肾细胞癌中的表达及其作用[J]. 山东大学学报(医学版), 2017, 55(3): 94-99.
[15].黄振, 荆涛, 骆磊, 刘勇. Xp11.2易位/TFE3基因融合相关性肾癌与肾透明细胞癌差异性分析[J]. 临床泌尿外科杂志, 2017, 17(03): 196-201.
[16].ZHANG H, XU W, LI B, et al. Curcumin promotes cell cycle arrest and inhibits survival of human renal cancer cells by negative modulation of the PI3K/AKT signaling pathway [J]. Cell Biochem Biophys, 2015, 73(3): 681-686. |
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