piRNA在胃癌与正常胃黏膜组织中表达的比较

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Abstract Objective: To analyze the differences of expression of piRNA in gastric carcinoma and normal gastric mucosa. Methods: The small RNA (sRNA) deep sequencing of gastric cancer tissue and normal gastric mucosa was performed by a new generation of high throughput sequencing technology, and the differential expression of piRNA was detected in gastric cancer tissue and normal gastric mucosa by follow-up bioinformatics. Four kinds of piRNAs (login ID: DQ570956, DQ575659, DQ594126 and DQ597128) were selected according to the results of the analysis, and their presence and their differences in gastric cancer tissues and normal gastric mucosa were verified by real-time quantitative PCR. Results: Solexa depth sequencing results showed that there was no significant difference in the proportion of sRNA measured in normal gastric mucosa and gastric cancer tissues (Z=0.835, P=0.678). The number of piRNAs measured in normal tissues was significantly higher than that in gastric cancer tissues (Z=2.167, P=0.042). Four piRNAs were selected according to the analysis results. The expression of that 4 piRNA in gastric cancer tissues was significantly lower in normal gastric mucosa (P<0.05). Conclusion: There are many differentially expressed piRNAs in gastric cancer tissues, and there is significant difference in the normal gastric mucosa. In this study, four piRNAs may be involved in the development and progression of gastric cancer, and the subsequent development of piRNA and gastric cancer of the pathogenesis of the study to provide experimental basis.

Key words： stomach neoplasms piRNA small RNA polymerase chain reaction

Received: 18 March 2017

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	Articles by authors
	JIANG Peipei
	CAI Yiqi
	WANG Pengfei
	CHEN Xiaodong
	JIN Jinji
	HU Changyuan
	CHEN Wenjing
	XUE Xiangyang
	ZHANG Lifang
	ZHU Guanbao

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I10/708

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