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| Observation on the process of vascular reconstruction in acellular hepatic scaffolds of rat after transplantation into the greater omentum |
| HUANG Junjie1, 3, WANG Zhibin1, CHEN Na2, ZHANG Jianse1, WANG Zhiyi1, LI Ting1, Dai Kaiyu1, MEI Jin1, LOU Xinfa1. |
| 1.Institute of Bioscaffold Transplantation and Immunology, Wenzhou Medical University, Wenzhou, 325035; 2.School of the Second Clinical Medical Sciences, Wenzhou Medical University, Wenzhou, 325035; 3.Department of General Surgery, the First Affiliated Hospital of Zhejiang University, Hangzhou, 310058 |
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HUANG Junjie,WANG Zhibin,CHEN Na, et al. Observation on the process of vascular reconstruction in acellular hepatic scaffolds of rat after transplantation into the greater omentum[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(10): 703-707.
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Abstract Objective: To observe the process of vascular reconstruction in acellular hepatic scaffolds of rat after transplantation into the greater omentum. Methods: Acellular hepatic scaffolds were harvested from adult SD rats by perfusing with TritonX-100. The scaffolds and native livers were observed through genomic DNA content analysis, scanning electron microscopy, HE stain, immunofluorescence and vascular cast. Then the acellular hepatic scaffolds were transplanted into the greater omentum in rats to investigate the vascular reconstruction. Rats were sacrificed respectively at 1, 3, 7, 14 or 28 days post-implantation and sections from the explanted grafts were analyzed by HE stain for morphology and microstructures. Results: Scanning electron microscope, HE stain and immunofluorescence showed most collagen fibers but no visible cell nuclei remained after decellularization in the scaffolds compared with the native livers. Vascular cast showed the pipeline structures of acellular hepatic scaffolds were intact and clear. Quantitative analysis of DNA content within the scaffolds showed only (211.6 ± 25.3) ng/µL. Then the blood sinus were observed on border of grafts at 3 days after transplantation into greater omentum. Afterwards the neovessels appeared, got into the internal of scaffolds, and formed microvascular network gradually in hepatic scaffolds after 14 days. The de novo microvascular network were still existed in hepatic scaffolds on 28 days. Conclusion: The implantation of greater omentum can promote the recellularization and eventually formation of vascularized reticular structures in the decellularized hepatic scaffolds.
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Received: 11 March 2017
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