丹酚酸A对大鼠脑缺血再灌注后细胞凋亡的保护作用及其机制

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Abstract Objective: To investigate the protective effect of salvianolic acid A (Sal A) against apoptosis in cerebral ischemia-reperfusion model and its mechanism. Methods: SD rats were randomly divided into sham operation group (sham group), middle cerebral artery ischemia reperfusion model (MCA-IR) group (MCA-IR group), Sal A pretreatment and MCA-IR group (Sal A+MCA-IR group). MCA-IR was established in MCA-IR group and Sal A+MCA-IR group in which rats were pre-treated with 1.0, 2.5, 5.0 mg/kg Sal A via intraperitoneal injection daily for 1 week. The artery were reperfused for 24 h and rats were sacrificed. TUNEL was used to assayed the cell apoptosis, immunohistochemistry was implied to test the expression of cPLA2 and Western blot was undergone to analyzed p-Akt and total Akt. Immunohistochemistry was used to analyze the expression of cPLA2. Results: The apoptosis rate and MDA level in Sal A+MCA-IR group was lower, but SOD was higher than that in MCA-IR group (P<0.05). The expression of p-Akt in Hippocampus of MCA-IR model decreased, while the expression of cPLA2 increased significantly compared with control group. 1.0, 2.5, 5.0 mg/kg Sal A pretreatment reversed the decrease of p-Akt and increase of cPLA2. Conclusion: Sal A inhibits the apoptosis through decreasing the expression of cPLA2 and activating Akt pathway signaling in MCA-IR model.

Key words： salvianolic acid A apoptosis protein kinase B ischemia-reperfusion rats

Received: 15 January 2017

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	PENG Xiao
	Fang Shiji
	Zheng Liyun
	Qiu Weiwen

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I9/674

[1] ANZELL A R, MAIZY R, PRZYKLENK K, et al. Mitochondrial quality control and disease: insights into ischemia-reperfusion injury[J]. Mol Neurobiol,2017. DOI: 10.1007/s12035-017-0503-9. [Epub ahead of print]
[2] SANDERSON T H, REYNOLDS C A, KUMAR R, et al. Molecular mechanisms of ischemia-reperfusion injury in brain: pivotal role of the mitochondrial membrane potential in reactive oxygen species generation[J]. Mol Neurobiol, 2013, 47(1): 9-23.
[3] SUN G Y, CHUANG D Y, ZONG Y, et al. Role of cytosolic phospholipase A2 in oxidative and inflammatory signaling pathways in different cell types in the central nervous system[J]. Mol Neurobiol, 2014, 50(1): 6-14.
[4] LESLIE C C. Cytosolic phospholipase A2: physiological function and role in disease[J]. J Lipid Res, 2015, 56(8): 1386-1402.
[5] MAHMOOD Q, WANG G F, WU G, et al. Salvianolic acid A inhibits calpain activation and eNOS uncoupling during focal cerebral ischemia in mice[J]. Phytomedicine, 2017, 25: 8-14.
[6] LI X L, FAN J P, LIU J X, et al. Salvianolic acid A protects neonatal cardiomyocytes against hypoxia/reoxygenation-induced injury by preserving mitochondrial function and activating Akt/GSK-3beta signals[J]. Chin J Integr Med, 2017. Doi: 10.1007/s11655-016-2747-z.[Epub ahead of print]
[7] 姜义娜, 田福荣, 林倩, 等. bFGF经鼻给药对大鼠缺血再灌注脑损伤的保护作用[J]. 温州医科大学学报, 2015, 45(8): 560-565.
[8] 郭丰, 陆晓微, 徐秋萍. 醒脑静与血塞通联合应用对大鼠脑缺血再灌注损伤的保护作用[J]. 中华医学杂志, 2010, 90(23): 1645-1647.
[9] HO J H, HONG C Y. Salvianolic acids: small compounds with multiple mechanisms for cardiovascular protection[J]. J Biomed Sci, 2011, 18: 30.
[10] WANG Y, CHEN G, YU X, et al. Salvianolic acid B ameliorates cerebral ischemia/reperfusion injury through inhibiting TLR4/MyD88 signaling pathway[J]. Inflammation, 2016, 39(4): 1503-1513.
[11] NITO C, KAMADA H, ENDO H, et al. Role of the p38 mitogen-activated protein kinase/cytosolic phospholipase A2 signaling pathway in blood-brain barrier disruption after focal cerebral ischemia and reperfusion[J]. J Cereb Blood Flow Metab, 2008, 28(10): 1686-1696.
[12] ZHANG J, BARASCH N, LI R C, et al. Inhibition of cytosolic phospholipase A(2) alpha protects against focal ischemic brain damage in mice[J]. Brain Res, 2012, 1471: 129-137.
[13] YAO H, HAN X, HAN X. The cardioprotection of the insulin-mediated PI3K/Akt/mTOR signaling pathway[J]. Am J Cardiovasc Drugs, 2014, 14(6): 433-442.
[14] ZHANG H, LIU Y Y, JIANG Q, et al. Salvianolic acid A protects RPE cells against oxidative stress through activation of Nrf2/HO-1 signaling[J]. Free Radic Biol Med, 2014, 69: 219-228.