|
|
|
| Evaluation of the inhibitory action and toxicity of elemene liposome combining ultrasound cavitation on the ectopic tumor of the nude mice model |
| WANG Jianzhong1, XU Yi2, LIU Pengcheng1, CHEN Haobo1 |
| 1.Department of Oncology, Wenzhou Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medicine University, Wenzhou, 325000; 2.Department of Pharmacy, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou, 325015 |
|
| Cite this article: |
|
WANG Jianzhong,XU Yi,LIU Pengcheng, et al. Evaluation of the inhibitory action and toxicity of elemene liposome combining ultrasound cavitation on the ectopic tumor of the nude mice model[J]. JOURNAL OF WEZHOU MEDICAL UNIVERSITY, 2017, 47(8): 571-575.
|
|
|
|
Abstract Objective: To evaluate the inhibitory action and toxicity of elemene liposome (EL) combining ultrasonic cavitation on the entopic tumor for the nude mice model. Methods: The inhibitory efficacy was studied using C6 cells bearing armpit tumor model. Mice were assigned to 5 groups (n=7) as follows: group 1 for saline, group 2 for EL, group 3 for EL+MB, group 4 for EL+US and group 5 for EL+MB+US. Mice were administered via tail vein at the dosage of 50 mg/kg amount to 0.1 mL/10 g (injection solution/mice weight), 1 time per 2 d, 3 times in all. Tumor volume, tumor weight change, body weight growth rate were all measued. The white blood cell count (WBC) at the end point was also tested. Results: Compared with EL, EL+MB+US showed higher tumor growth inhibition, fewer mice body weight change and white blood count change (P<0.05) in the armpit tumor model. Conclusion: With better anti-tumor activity and lower blood toxicity, EL+MB+US may be developed as a new cancer targeted delivery system.
|
|
Received: 15 September 2016
|
| |
|
|
|
[1] 谢恬, 李铖璐, 王淑玲, 等. 榄香烯脂质体系列靶向抗癌天然药物基础研究进展[J]. 中国中西医结合杂志, 2014, 34(4): 507-512.
[2] CHEN J J, DUAN Y Y, HUANG M. Synthesis of 13-beta-elemene ester derivatives and evaluation of their antioxidant activity in human umbilical vein endothelial cells[J]. Chin J Nat Med, 2015, 13(8): 618-627.
[3] ZHAO Y Z, LIANG H D, MEI X G, et al. Preparation, characterization and in vivo observation of phospholipid-based gas-filled microbubbles containing hirudin[J]. UltrasoundMed Biol, 2005, 31(9): 1237-1243.
[4] ZHAO Y Z, LIN Q, WONG H L, et al. Glioma-targeted therapy using Cilengitide nanoparticles combined with UT-MD enhanced delivery[J]. J Control Release, 2016, 224:112-125.
[5] 马源, 陈俊杰, 陈成水, 等. STAT1基因转染对人肺腺癌裸鼠移植瘤生长的影响[J]. 温州医学院学报, 2013, 43(7): 461-463.
[6] ZHAO Y Z, LU C T, ZHOU Z C, et al. Enhancing chemotherapeutic drug inhibition on tumor growth by ultrasound:an in vivo experiment[J]. J Drug Target, 2011, 19(2): 154-160.
[7] 朱雁林, 傅红兴, 周伟忠, 等. 雷帕霉素栓剂的制备及质量评价[J]. 温州医科大学学报,2014, 44(2): 148-150.
[8] 陈渊, 王伟, 涂明, 等. siRNA靶向沉默成纤维细胞激活蛋白α对胶质瘤细胞U87增殖的影响[J]. 温州医科大学学报, 2014, 44(4): 241-244.
[9] LU J J, DANG Y Y, HUANG M, et al. Anti-cancer properties of terpenoids isolated from Rhizoma Curcumae-a review[J]. J Ethnopharmacol, 2012, 143(2): 406-411.
[10] 丁尚伟, 黄晓宇, 姚梦云, 等. 超声靶向转染缺氧诱导因子干扰基因治疗大鼠肝癌的研究[J]. 华中科技大学学报(医学版), 2014, 43(1): 12-16.
[11] FIDLER I J. Biological behavior of malignant melanoma cells correlated to their survival in vivo[J]. Cancer Res,1975, 35(1): 218-224.
[12] MINKO T, BATRAKOVA E V, LI S, et al. Pluronic block copolymers alter apoptotic signal transduction of doxorubicin in drug-resistant cancer cells[J]. J Control Release, 2005, 105(3): 269-278. |
|
|
|
