斜纹夜蛾嗅觉反应昼夜节律及其调控机制

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Abstract Objective: To study circadian rhythms of olfactory response and its regulatory mechanisms in Spodoptera litura, providing new insight in controlling this pest. Methods: Electroantennogram (EAG) was used to determine the electrophysiological responses of S. litura to chemicals originated from sex pheromones and host plants at eight different time points during day, finding the circadian rhythms of EAG responses in S. litura and selecting out the compounds to which the moths strongly responded. Then the wind tunnel was used to measure the behavioral responses of S. litura to these compounds at the same five time points during the scotophase. In addition, the expression levels of genes regulating circadian rhythms and olfaction at the same time points were detected by the real-time fluorescence quantitative PCR (RT-qPCR) technique to reveal the molecular regulatory mechanism of olfactory circadian rhythms in S. litura. Results: The results showed that the strongest EAG responses of male S. litura to all tested plant odor compounds and sex pheromone components occurred after 3 hours and 9 hours in the dark, showing a bimodal pattern. A total of five compounds: Z9E12-14:OAc, Z9E11-14:OAc,
caryophyllene, linalool and phenylacetaldehyde were selected and tested in behavioral responses (wind tunnel). The behavioral responses of S. litura to the five chemicals at five time points during scotophase showed similar patterns to that obtained from the EAG tests. The expression levels of the most olfactory receptor and clock genes reached the peak after 3 and 9 hours in the dark, which is largely consistent with the circadian rhythms of olfactory responses in S. litura. Conclusion: The circadian rhythms of olfactory responses were consistent with those of expression levels of olfactory and clock genes in S. litura, suggesting that the circadian rhythms are probably essentially regulated by clock and olfactory genes.

Key words： Spodoptera litura circadian rhythms electroantennogram wind tunnel RT-qPCR

Received: 02 November 2016

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	LIN Huiyue
	QIAN Kai
	BAI Jiaxiu
	ZHANG Daogen
	LU Rui
	WAN Xinlong.

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https://xb.wmu.edu.cn/EN/ OR https://xb.wmu.edu.cn/EN/Y2017/V47/I8/553

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